Reviewed, UniProtKB/Swiss-Prot Q7ZWR2 (SYCC_XENLA)

Names and origin

Protein names

Recommended name:
    Cysteinyl-tRNA synthetase, cytoplasmic
    EC=6.1.1.16
Alternative name(s):
    Cysteine--tRNA ligase
      Short name=CysRS

Gene names

Name:

cars

Organism

Xenopus laevis (African clawed frog)

Taxonomic identifier

8355 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Amphibia › Batrachia › Anura › Mesobatrachia › Pipoidea › Pipidae › Xenopodinae › Xenopus › Xenopus

Protein attributes

Sequence length	747 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at transcript level.

General annotation (Comments)

Catalytic activity	ATP + L-cysteine + tRNA(Cys) = AMP + diphosphate + L-cysteinyl-tRNA(Cys).
Cofactor	Binds 1 zinc ion per subunit By similarity.
Subcellular location	Cytoplasm By similarity.
Sequence similarities	Belongs to the class-I aminoacyl-tRNA synthetase family.

Ontologies

Keywords
Biological process	Protein biosynthesis
Cellular component	Cytoplasm
Ligand	ATP-binding Metal-binding Nucleotide-binding Zinc
Molecular function	Aminoacyl-tRNA synthetase Ligase
Gene Ontology (GO)
Biological process	cysteinyl-tRNA aminoacylation Inferred from sequence or structural similarity. Source: UniProtKB
Cellular component	cytoplasm Inferred from sequence or structural similarity. Source: UniProtKB
Molecular function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW cysteine-tRNA ligase activity Inferred from sequence or structural similarity. Source: UniProtKB tRNA binding Inferred from sequence or structural similarity. Source: UniProtKB zinc ion binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 747	747	Cysteinyl-tRNA synthetase, cytoplasmic		PRO_0000250746
Regions
	Motif	56 – 66	11	"HIGH" region
	Motif	405 – 409	5	"KMSKS" region
Sites
	Metal binding	54	1	Zinc By similarity
	Metal binding	347	1	Zinc By similarity
	Metal binding	372	1	Zinc By similarity
	Metal binding	376	1	Zinc By similarity
	Binding site	408	1	ATP By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q7ZWR2-1 [UniParc]. Last modified June 1, 2003. Version 1. Checksum: D76FAB9E68059AC2	FASTA	747	85,784
	10 20 30 40 50 60 MTESWEQGKG RRTQPPWSAP NTNEQPGLRL YNSLTRSKEL FFPQVGRKVT WYCCGPTVYD 70 80 90 100 110 120 ASHMGHARSY ISFDILRRVL RDYFKYDVFY CMNITDIDDK IIKRARQRHL FQQYRESNPP 130 140 150 160 170 180 HSDLLQDVNT ALIPFLQRIS ETNDPDKKQM LERIQTAVSA ALLPLQDALN KNTGAEELQK 190 200 210 220 230 240 HSQVLMEAAV DLLSDWLDEK HGAQIADNSI FSQLPKYWEG EYHKDMEALN VLTPDVLTRV 250 260 270 280 290 300 SEYVPEIVAF VQKIVDNGYG YVSNGSVYFS TAKFHDSENH FYAKLVPEAV GDQKALQEGE 310 320 330 340 350 360 GDLSISADRL SEKQSPNDFA LWKASKPGEP SWESPWGKGR PGWHIECSAM AGSILGESMD 370 380 390 400 410 420 IHGGGFDLRF PHHDNELAQS EAYFDNDHWV RYFLHTGHLT IAGCKMSKSL KNFITIKDAL 430 440 450 460 470 480 QKNTARQLRL AFLMHSWKDT LDYSNNTMES AVQYEKFMNE FFLNVKDLLR APTDITGQFV 490 500 510 520 530 540 KWEVLEIELN NCFYSKKAAI HEALCDNIDT RTVMEEMRSL VSQCNSYIAS KKVAKQFPNR 550 560 570 580 590 600 MLLRSISSYL TSMLKVFGAI EGEEVIGFPI GGSENSMNLE STVMPYLQVL SQFREGVRQI 610 620 630 640 650 660 ARQHKVTEVL QLSDLLRDDI LPELGVRLED HEGLPTVVKL VDRETLLKEK EEKRKAEEEK 670 680 690 700 710 720 QRKKEEAARK KQQQEAAKLE KMKISPSQMF TLETDKYSQF DESGFPTHDT EGKELSKGQT 730 740 KKLRKLFEVQ EKLHKEYLQM VQNGTTA

References

[1]	NIH - Xenopus Gene Collection (XGC) project Submitted (FEB-2003) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Tissue: Embryo.

Cross-references

Sequence databases
EMBLGenBankDDBJ	BC046746 mRNA. Translation: AAH46746.1.
RefSeq	NP_001080786.1.
UniGene	Xl.13786
3D structure databases
HSSP	HSSP built from PDB template 1LI5 based on UniProtKB P21888.
ModBase	Search...
Genome annotation databases
GeneID	380479.
KEGG	xla:380479.
Organism-specific databases
CTD	380479.
Xenbase	XB-FEAT-5825932. cars.
Phylogenomic databases
HOVERGEN	Q7ZWR2.
Enzyme and pathway databases
BRENDA	6.1.1.16. 648.
Family and domain databases
InterPro	IPR001412. aa-tRNA-synth_I_CS. IPR015804. Cys-tRNA-synt_Ia_C. IPR015803. Cys-tRNA-synt_Ia_N. IPR002308. Cys-tRNA-synth_1a. IPR014729. Rossmann-like_a/b/a_fold. [Graphical view]
Gene3D	G3DSA:3.40.50.620. Rossmann-like_a/b/a_fold. 1 hit.
PANTHER	PTHR10890. Cys_tRNA-synt_1a. 1 hit.
Pfam	PF01406. tRNA-synt_1e. 1 hit. [Graphical view]
PRINTS	PR00983. TRNASYNTHCYS.
TIGRFAMs	TIGR00435. cysS. 1 hit.
PROSITE	PS00178. AA_TRNA_LIGASE_I. False negative. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

SYCC_XENLA

Accession

Primary (citable) accession number: Q7ZWR2

Entry history

Integrated into UniProtKB/Swiss-Prot:	October 3, 2006
Last sequence update:	June 1, 2003
Last modified:	September 1, 2009
This is version 43 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

Xenopus annotation project