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Protein

Argininosuccinate synthase

Gene

ass1

Organism
Xenopus laevis (African clawed frog)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Experimental evidence at transcript leveli

Functioni

Catalytic activityi

ATP + L-citrulline + L-aspartate = AMP + diphosphate + N(omega)-(L-arginino)succinate.

Pathwayi

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei37 – 371ATP; via amide nitrogen and carbonyl oxygenBy similarity
Binding sitei88 – 881CitrullineBy similarity
Binding sitei93 – 931CitrullineBy similarity
Binding sitei120 – 1201AspartateBy similarity
Binding sitei124 – 1241AspartateBy similarity
Binding sitei124 – 1241CitrullineBy similarity
Binding sitei125 – 1251AspartateBy similarity
Binding sitei128 – 1281CitrullineBy similarity
Binding sitei181 – 1811CitrullineBy similarity
Binding sitei190 – 1901CitrullineBy similarity
Binding sitei271 – 2711CitrullineBy similarity
Binding sitei283 – 2831CitrullineBy similarity

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi11 – 199ATPBy similarity
Nucleotide bindingi116 – 1249ATPBy similarity

GO - Molecular functioni

  1. argininosuccinate synthase activity Source: UniProtKB-EC
  2. ATP binding Source: UniProtKB-KW

GO - Biological processi

  1. arginine biosynthetic process Source: UniProtKB-UniPathway
  2. urea cycle Source: UniProtKB-UniPathway
Complete GO annotation...

Keywords - Molecular functioni

Ligase

Keywords - Biological processi

Amino-acid biosynthesis, Arginine biosynthesis, Urea cycle

Keywords - Ligandi

ATP-binding, Nucleotide-binding

Enzyme and pathway databases

UniPathwayiUPA00068; UER00113.
UPA00158; UER00272.

Names & Taxonomyi

Protein namesi
Recommended name:
Argininosuccinate synthase (EC:6.3.4.5)
Alternative name(s):
Citrulline--aspartate ligase
Gene namesi
Name:ass1
Synonyms:ass
OrganismiXenopus laevis (African clawed frog)
Taxonomic identifieri8355 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiAmphibiaBatrachiaAnuraPipoideaPipidaeXenopodinaeXenopusXenopus

Organism-specific databases

XenbaseiXB-GENE-977789. ass1.

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 411411Argininosuccinate synthasePRO_0000321324Add
BLAST

Interactioni

Subunit structurei

Homotetramer.By similarity

Structurei

3D structure databases

ProteinModelPortaliQ7ZWM4.
SMRiQ7ZWM4. Positions 5-407.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the argininosuccinate synthase family.Curated

Phylogenomic databases

HOVERGENiHBG001717.
KOiK01940.

Family and domain databases

Gene3Di3.40.50.620. 1 hit.
3.90.1260.10. 1 hit.
HAMAPiMF_00005. Arg_succ_synth_type1.
InterProiIPR001518. Arginosuc_synth.
IPR018223. Arginosuc_synth_CS.
IPR023434. Arginosuc_synth_type_1_subfam.
IPR024074. AS_cat/multimer_dom_body.
IPR014729. Rossmann-like_a/b/a_fold.
[Graphical view]
PfamiPF00764. Arginosuc_synth. 1 hit.
[Graphical view]
TIGRFAMsiTIGR00032. argG. 1 hit.
PROSITEiPS00564. ARGININOSUCCIN_SYN_1. 1 hit.
PS00565. ARGININOSUCCIN_SYN_2. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q7ZWM4-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MSQSKGTVVL AYSGGLDTSC ILVWLKEQGF DVIAYLANIG QNEDFEEARK
60 70 80 90 100
KAVNLGAKKV YIEDIRQQFV EEYIWPAVQA NAIYEDRYLL GTSLARPCIA
110 120 130 140 150
KKQVEIAKKE AAEYVSHGAT GKGNDQIRFE LTCYSLYPEV KIIAPWRMPE
160 170 180 190 200
FYNRFRGRSD LMEYAKKHNI SVPVTPKSPW SMDENLMHIS YEGGILENPK
210 220 230 240 250
NHAPPGLYLK TKDPATSPDE PDILEIEFKK GVPVKVTNTK NKTQHSSSLA
260 270 280 290 300
LFCYLNEVAG KHGVGRIDIV ENRFIGMKSR GIYETPAGTI LYQAHLDIEA
310 320 330 340 350
FTMDREMRKI KQQLSQRFAE QIYNGFWYSP ECEFVRSCIS KSQEMVEGKV
360 370 380 390 400
LVSVLKGQVY VLGREAPHSL YNEELVSMDV QGDYDPADAC GFIKINAVRL
410
KEYHRLQKNK K
Length:411
Mass (Da):46,750
Last modified:June 1, 2003 - v1
Checksum:i9A9F683A38A2378C
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
BC046941 mRNA. Translation: AAH46941.1.
RefSeqiNP_001080795.1. NM_001087326.1.
UniGeneiXl.17580.

Genome annotation databases

GeneIDi380488.
KEGGixla:380488.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
BC046941 mRNA. Translation: AAH46941.1.
RefSeqiNP_001080795.1. NM_001087326.1.
UniGeneiXl.17580.

3D structure databases

ProteinModelPortaliQ7ZWM4.
SMRiQ7ZWM4. Positions 5-407.
ModBaseiSearch...
MobiDBiSearch...

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

GeneIDi380488.
KEGGixla:380488.

Organism-specific databases

CTDi445.
XenbaseiXB-GENE-977789. ass1.

Phylogenomic databases

HOVERGENiHBG001717.
KOiK01940.

Enzyme and pathway databases

UniPathwayiUPA00068; UER00113.
UPA00158; UER00272.

Family and domain databases

Gene3Di3.40.50.620. 1 hit.
3.90.1260.10. 1 hit.
HAMAPiMF_00005. Arg_succ_synth_type1.
InterProiIPR001518. Arginosuc_synth.
IPR018223. Arginosuc_synth_CS.
IPR023434. Arginosuc_synth_type_1_subfam.
IPR024074. AS_cat/multimer_dom_body.
IPR014729. Rossmann-like_a/b/a_fold.
[Graphical view]
PfamiPF00764. Arginosuc_synth. 1 hit.
[Graphical view]
TIGRFAMsiTIGR00032. argG. 1 hit.
PROSITEiPS00564. ARGININOSUCCIN_SYN_1. 1 hit.
PS00565. ARGININOSUCCIN_SYN_2. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

  1. NIH - Xenopus Gene Collection (XGC) project
    Submitted (FEB-2003) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Tissue: Embryo.

Entry informationi

Entry nameiASSY_XENLA
AccessioniPrimary (citable) accession number: Q7ZWM4
Entry historyi
Integrated into UniProtKB/Swiss-Prot: February 26, 2008
Last sequence update: June 1, 2003
Last modified: October 1, 2014
This is version 66 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.