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Q7ZWK8

- OTOP1_DANRE

UniProt

Q7ZWK8 - OTOP1_DANRE

Protein

Otopetrin-1

Gene

otop1

Organism
Danio rerio (Zebrafish) (Brachydanio rerio)
Status
Reviewed - Annotation score: 3 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 54 (01 Oct 2014)
      Sequence version 2 (15 Jan 2008)
      Previous versions | rss
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    Functioni

    Inhibits P2Y purinoceptors. Modulates calcium homeostasis and influx of calcium in response to extracellular ATP By similarity. Essential for the formation of otoliths in the inner ear of developing larvae and for the perception of gravity and acceleration.By similarity2 Publications

    GO - Biological processi

    1. biomineral tissue development Source: UniProtKB-KW

    Keywords - Biological processi

    Biomineralization

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Otopetrin-1
    Gene namesi
    Name:otop1
    OrganismiDanio rerio (Zebrafish) (Brachydanio rerio)
    Taxonomic identifieri7955 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiActinopterygiiNeopterygiiTeleosteiOstariophysiCypriniformesCyprinidaeDanio
    ProteomesiUP000000437: Unplaced

    Subcellular locationi

    Membrane 1 Publication; Multi-pass membrane protein 1 Publication. Secretedextracellular space By similarity

    GO - Cellular componenti

    1. extracellular space Source: UniProtKB-SubCell
    2. integral component of membrane Source: UniProtKB-KW

    Keywords - Cellular componenti

    Membrane, Secreted

    Pathology & Biotechi

    Disruption phenotypei

    Fishes display the backstroke phenotype. Mutant fish exhibit otolith agenesis without additional effects on the sensory epithelium or other inner ear structures. Otoliths are essential for the perception of gravity and acceleration. Larvae without otoliths are unable to orient dorsal side up and rest mostly on their side in contact with the ground.2 Publications

    Mutagenesis

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Mutagenesisi429 – 4291E → V in backstroke; causes otholith agenesis. 1 Publication

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 586586Otopetrin-1PRO_0000313819Add
    BLAST

    Expressioni

    Developmental stagei

    First detected in the otic placode at 16.5 hours post-fertilization (hpf). Detected in the developing inner ear sensory epithelium at 24 hpf. Detected in hair cells of the sensory epithelium at 4 days post-fertilization (dpf). At 5 dpf, levels are much reduced in the otolith organs, and not detectable at 7 dpf. Detected in neuromasts and in the lateral line system at 5 dpf and 7 dpf.2 Publications

    Structurei

    Transmembrane

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Transmembranei54 – 7421HelicalSequence AnalysisAdd
    BLAST
    Transmembranei86 – 10621HelicalSequence AnalysisAdd
    BLAST
    Transmembranei124 – 14421HelicalSequence AnalysisAdd
    BLAST
    Transmembranei146 – 16621HelicalSequence AnalysisAdd
    BLAST
    Transmembranei195 – 21521HelicalSequence AnalysisAdd
    BLAST
    Transmembranei260 – 28021HelicalSequence AnalysisAdd
    BLAST
    Transmembranei303 – 32321HelicalSequence AnalysisAdd
    BLAST
    Transmembranei342 – 36221HelicalSequence AnalysisAdd
    BLAST
    Transmembranei519 – 53921HelicalSequence AnalysisAdd
    BLAST
    Transmembranei551 – 57121HelicalSequence AnalysisAdd
    BLAST

    Family & Domainsi

    Sequence similaritiesi

    Belongs to the otopetrin family.Curated

    Keywords - Domaini

    Transmembrane, Transmembrane helix

    Phylogenomic databases

    eggNOGiNOG259421.
    HOVERGENiHBG108223.
    PhylomeDBiQ7ZWK8.

    Family and domain databases

    InterProiIPR004878. Otopetrin.
    [Graphical view]
    PANTHERiPTHR21522. PTHR21522. 1 hit.
    PfamiPF03189. Otopetrin. 3 hits.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Q7ZWK8-1 [UniParc]FASTAAdd to Basket

    « Hide

    MVEHGGTDSM WLNKYNPAAA SSASSSSSSD AENKLFSRLK VSLTKKYPQK    50
    NAELLSAQYG TNLLLLGVSV MLALAAQSGP VKEEHLLSFI TVLMLVQLVW 100
    MLCYMIRRER ERSPVPERDA HAGASWIRGG LTMLALLSLI MDAFRIGYFV 150
    GYHSCISAAL GVYPIVHALH TISQVHFLWF HIKDVIKKYE TFERFGVIHA 200
    VFTNLLLWCN GVMSETEHFM HNHRRRLIEM GYANLSTVDV QPHCNCTTSV 250
    CSMFSTSLYY LYPFNIEYHI FVSAMLFVMW KNIGRTLDRH SNRKRRSTGS 300
    TGLLLGPLGG LVALASSVSV LVVYLIHLEK TEEMHEAAVS MFYYYGVAMM 350
    ACMCVGSGTG LLVYRMENRP MDTGSNPART LDTELLLASS LGSWLMSWCS 400
    VVASVAEAGQ KSPSFSWTSL TYSLLLVLEK CIQNLFIVES LYRRHSEEEE 450
    DAAAPQVFSV AVPPYDGILN HGYEAHDKHR EAEPAAGSHA LSRKQPDAPL 500
    PAGQRLDVTP GRKRQILKNI CMFLFMCNIS LWILPAFGCR PQYDNPLENE 550
    TFGTSVWTTV LNVAIPLNLF YRMHSVASLF EVFRKV 586
    Length:586
    Mass (Da):65,721
    Last modified:January 15, 2008 - v2
    Checksum:i000ED7A4E690831E
    GO

    Experimental Info

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sequence conflicti171 – 1711T → A in AAH83445. 1 PublicationCurated
    Sequence conflicti251 – 2522CS → WL in AAN62756. (PubMed:12651873)Curated
    Sequence conflicti300 – 3001S → T in AAH83445. 1 PublicationCurated

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AY170648 mRNA. Translation: AAN62756.1.
    AY744157 mRNA. Translation: AAV91780.1.
    BC083445 mRNA. Translation: AAH83445.1.
    RefSeqiNP_942098.1. NM_198803.1.
    UniGeneiDr.77257.

    Genome annotation databases

    GeneIDi322893.
    KEGGidre:322893.

    Cross-referencesi

    Web resourcesi

    Protein Spotlight

    Ear of stone - Issue 89 of December 2007

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AY170648 mRNA. Translation: AAN62756.1 .
    AY744157 mRNA. Translation: AAV91780.1 .
    BC083445 mRNA. Translation: AAH83445.1 .
    RefSeqi NP_942098.1. NM_198803.1.
    UniGenei Dr.77257.

    3D structure databases

    ModBasei Search...
    MobiDBi Search...

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    GeneIDi 322893.
    KEGGi dre:322893.

    Organism-specific databases

    CTDi 133060.

    Phylogenomic databases

    eggNOGi NOG259421.
    HOVERGENi HBG108223.
    PhylomeDBi Q7ZWK8.

    Miscellaneous databases

    NextBioi 20807980.

    Family and domain databases

    InterProi IPR004878. Otopetrin.
    [Graphical view ]
    PANTHERi PTHR21522. PTHR21522. 1 hit.
    Pfami PF03189. Otopetrin. 3 hits.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Non-syndromic vestibular disorder with otoconial agenesis in tilted/mergulhador mice caused by mutations in otopetrin 1."
      Hurle B., Ignatova E., Massironi S.M., Mashimo T., Rios X., Thalmann I., Thalmann R., Ornitz D.M.
      Hum. Mol. Genet. 12:777-789(2003) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA].
      Tissue: Embryo.
    2. "Mutated otopetrin 1 affects the genesis of otoliths and the localization of Starmaker in zebrafish."
      Soellner C., Schwarz H., Geisler R., Nicolson T.
      Dev. Genes Evol. 214:582-590(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA], FUNCTION, DEVELOPMENTAL STAGE, SUBCELLULAR LOCATION, MUTAGENESIS OF GLU-429, DISRUPTION PHENOTYPE.
    3. NIH - Zebrafish Gene Collection (ZGC) project
      Submitted (OCT-2004) to the EMBL/GenBank/DDBJ databases
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
      Tissue: Larva.
    4. Cited for: FUNCTION, DEVELOPMENTAL STAGE, DISRUPTION PHENOTYPE.

    Entry informationi

    Entry nameiOTOP1_DANRE
    AccessioniPrimary (citable) accession number: Q7ZWK8
    Secondary accession number(s): Q5MKL8, Q5XJ61
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: January 15, 2008
    Last sequence update: January 15, 2008
    Last modified: October 1, 2014
    This is version 54 of the entry and version 2 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program

    Miscellaneousi

    Miscellaneous

    Otoliths are structures composed of proteins and calcium carbonate that are found in the fish inner ear in contact with the otic epithelium. They are essential for the perception of gravity and acceleration.

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. Protein Spotlight
      Protein Spotlight articles and cited UniProtKB/Swiss-Prot entries
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3