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Q7ZWK8 (OTOP1_DANRE) Reviewed, UniProtKB/Swiss-Prot

Last modified April 16, 2014. Version 53. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Web links·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Otopetrin-1
Gene names
Name:otop1
OrganismDanio rerio (Zebrafish) (Brachydanio rerio) [Reference proteome]
Taxonomic identifier7955 [NCBI]
Taxonomic lineageEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiActinopterygiiNeopterygiiTeleosteiOstariophysiCypriniformesCyprinidaeDanio

Protein attributes

Sequence length586 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Inhibits P2Y purinoceptors. Modulates calcium homeostasis and influx of calcium in response to extracellular ATP By similarity. Essential for the formation of otoliths in the inner ear of developing larvae and for the perception of gravity and acceleration. Ref.2 Ref.4

Subcellular location

Membrane; Multi-pass membrane protein. Secretedextracellular space By similarity Ref.2.

Developmental stage

First detected in the otic placode at 16.5 hours post-fertilization (hpf). Detected in the developing inner ear sensory epithelium at 24 hpf. Detected in hair cells of the sensory epithelium at 4 days post-fertilization (dpf). At 5 dpf, levels are much reduced in the otolith organs, and not detectable at 7 dpf. Detected in neuromasts and in the lateral line system at 5 dpf and 7 dpf. Ref.2 Ref.4

Disruption phenotype

Fishes display the backstroke phenotype. Mutant fish exhibit otolith agenesis without additional effects on the sensory epithelium or other inner ear structures. Otoliths are essential for the perception of gravity and acceleration. Larvae without otoliths are unable to orient dorsal side up and rest mostly on their side in contact with the ground. Ref.2 Ref.4

Miscellaneous

Otoliths are structures composed of proteins and calcium carbonate that are found in the fish inner ear in contact with the otic epithelium. They are essential for the perception of gravity and acceleration.

Sequence similarities

Belongs to the otopetrin family.

Ontologies

Keywords
   Biological processBiomineralization
   Cellular componentMembrane
Secreted
   DomainTransmembrane
Transmembrane helix
   Technical termComplete proteome
Reference proteome
Gene Ontology (GO)
   Biological_processbiomineral tissue development

Inferred from electronic annotation. Source: UniProtKB-KW

   Cellular_componentextracellular space

Inferred from electronic annotation. Source: UniProtKB-SubCell

integral component of membrane

Inferred from electronic annotation. Source: UniProtKB-KW

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 586586Otopetrin-1
PRO_0000313819

Regions

Transmembrane54 – 7421Helical; Potential
Transmembrane86 – 10621Helical; Potential
Transmembrane124 – 14421Helical; Potential
Transmembrane146 – 16621Helical; Potential
Transmembrane195 – 21521Helical; Potential
Transmembrane260 – 28021Helical; Potential
Transmembrane303 – 32321Helical; Potential
Transmembrane342 – 36221Helical; Potential
Transmembrane519 – 53921Helical; Potential
Transmembrane551 – 57121Helical; Potential

Experimental info

Mutagenesis4291E → V in backstroke; causes otholith agenesis. Ref.2
Sequence conflict1711T → A in AAH83445. Ref.3
Sequence conflict251 – 2522CS → WL in AAN62756. Ref.1
Sequence conflict3001S → T in AAH83445. Ref.3

Sequences

Sequence LengthMass (Da)Tools
Q7ZWK8 [UniParc].

Last modified January 15, 2008. Version 2.
Checksum: 000ED7A4E690831E

FASTA58665,721
        10         20         30         40         50         60 
MVEHGGTDSM WLNKYNPAAA SSASSSSSSD AENKLFSRLK VSLTKKYPQK NAELLSAQYG 

        70         80         90        100        110        120 
TNLLLLGVSV MLALAAQSGP VKEEHLLSFI TVLMLVQLVW MLCYMIRRER ERSPVPERDA 

       130        140        150        160        170        180 
HAGASWIRGG LTMLALLSLI MDAFRIGYFV GYHSCISAAL GVYPIVHALH TISQVHFLWF 

       190        200        210        220        230        240 
HIKDVIKKYE TFERFGVIHA VFTNLLLWCN GVMSETEHFM HNHRRRLIEM GYANLSTVDV 

       250        260        270        280        290        300 
QPHCNCTTSV CSMFSTSLYY LYPFNIEYHI FVSAMLFVMW KNIGRTLDRH SNRKRRSTGS 

       310        320        330        340        350        360 
TGLLLGPLGG LVALASSVSV LVVYLIHLEK TEEMHEAAVS MFYYYGVAMM ACMCVGSGTG 

       370        380        390        400        410        420 
LLVYRMENRP MDTGSNPART LDTELLLASS LGSWLMSWCS VVASVAEAGQ KSPSFSWTSL 

       430        440        450        460        470        480 
TYSLLLVLEK CIQNLFIVES LYRRHSEEEE DAAAPQVFSV AVPPYDGILN HGYEAHDKHR 

       490        500        510        520        530        540 
EAEPAAGSHA LSRKQPDAPL PAGQRLDVTP GRKRQILKNI CMFLFMCNIS LWILPAFGCR 

       550        560        570        580 
PQYDNPLENE TFGTSVWTTV LNVAIPLNLF YRMHSVASLF EVFRKV 

« Hide

References

« Hide 'large scale' references
[1]"Non-syndromic vestibular disorder with otoconial agenesis in tilted/mergulhador mice caused by mutations in otopetrin 1."
Hurle B., Ignatova E., Massironi S.M., Mashimo T., Rios X., Thalmann I., Thalmann R., Ornitz D.M.
Hum. Mol. Genet. 12:777-789(2003) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA].
Tissue: Embryo.
[2]"Mutated otopetrin 1 affects the genesis of otoliths and the localization of Starmaker in zebrafish."
Soellner C., Schwarz H., Geisler R., Nicolson T.
Dev. Genes Evol. 214:582-590(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA], FUNCTION, DEVELOPMENTAL STAGE, SUBCELLULAR LOCATION, MUTAGENESIS OF GLU-429, DISRUPTION PHENOTYPE.
[3]NIH - Zebrafish Gene Collection (ZGC) project
Submitted (OCT-2004) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
Tissue: Larva.
[4]"Otopetrin 1 is required for otolith formation in the zebrafish Danio rerio."
Hughes I., Blasiole B., Huss D., Warchol M.E., Rath N.P., Hurle B., Ignatova E., Dickman J.D., Thalmann R., Levenson R., Ornitz D.M.
Dev. Biol. 276:391-402(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: FUNCTION, DEVELOPMENTAL STAGE, DISRUPTION PHENOTYPE.

Web resources

Protein Spotlight

Ear of stone - Issue 89 of December 2007

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AY170648 mRNA. Translation: AAN62756.1.
AY744157 mRNA. Translation: AAV91780.1.
BC083445 mRNA. Translation: AAH83445.1.
RefSeqNP_942098.1. NM_198803.1.
UniGeneDr.77257.

3D structure databases

ModBaseSearch...
MobiDBSearch...

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

GeneID322893.
KEGGdre:322893.

Organism-specific databases

CTD133060.

Phylogenomic databases

eggNOGNOG259421.
HOVERGENHBG108223.
PhylomeDBQ7ZWK8.

Family and domain databases

InterProIPR004878. Otopetrin.
[Graphical view]
PANTHERPTHR21522. PTHR21522. 1 hit.
PfamPF03189. Otopetrin. 3 hits.
[Graphical view]
ProtoNetSearch...

Other

NextBio20807980.

Entry information

Entry nameOTOP1_DANRE
AccessionPrimary (citable) accession number: Q7ZWK8
Secondary accession number(s): Q5MKL8, Q5XJ61
Entry history
Integrated into UniProtKB/Swiss-Prot: January 15, 2008
Last sequence update: January 15, 2008
Last modified: April 16, 2014
This is version 53 of the entry and version 2 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

Protein Spotlight

Protein Spotlight articles and cited UniProtKB/Swiss-Prot entries