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Protein

Proton channel OTOP1

Gene

otop1

Organism
Danio rerio (Zebrafish) (Brachydanio rerio)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Proton-selective channel that specifically transports protons into cells (By similarity). Proton channel activity is only weakly-sensitive to voltage (By similarity). Proton-selective channel activity is probably required in cell types that use changes in intracellular pH for cell signaling or to regulate biochemical or developmental processes (By similarity). In the vestibular system of the inner ear, required for the formation and function of otoconia, which are calcium carbonate crystals that sense gravity and acceleration (PubMed:15480759, PubMed:15581873). Probably acts by maintaining the pH appropriate for formation of otoconia (By similarity). May be involved in sour taste perception in sour taste cells by mediating entry of protons within the cytosol (By similarity).By similarity2 Publications

GO - Molecular functioni

GO - Biological processi

  • cellular response to insulin stimulus Source: UniProtKB
  • inner ear morphogenesis Source: ZFIN
  • negative regulation of interferon-gamma-mediated signaling pathway Source: UniProtKB
  • otolith formation Source: ZFIN
  • otolith mineralization Source: ZFIN
  • proton transmembrane transport Source: UniProtKB

Keywordsi

Molecular functionIon channel
Biological processBiomineralization, Hydrogen ion transport, Ion transport, Transport

Protein family/group databases

TCDBi1.A.110.1.5 the channel-forming otopetrin (otop) family

Names & Taxonomyi

Protein namesi
Recommended name:
Proton channel OTOP1Curated
Alternative name(s):
Otopetrin-11 Publication
Gene namesi
Name:otop11 Publication
OrganismiDanio rerio (Zebrafish) (Brachydanio rerio)
Taxonomic identifieri7955 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiActinopterygiiNeopterygiiTeleosteiOstariophysiCypriniformesCyprinidaeDanio
Proteomesi
  • UP000000437 Componenti: Unplaced

Organism-specific databases

ZFINiZDB-GENE-030131-1613 otop1

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Topology

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Transmembranei54 – 74HelicalSequence analysisAdd BLAST21
Transmembranei86 – 106HelicalSequence analysisAdd BLAST21
Transmembranei124 – 144HelicalSequence analysisAdd BLAST21
Transmembranei146 – 166HelicalSequence analysisAdd BLAST21
Transmembranei167 – 180HelicalSequence analysisAdd BLAST14
Transmembranei195 – 215HelicalSequence analysisAdd BLAST21
Transmembranei260 – 280HelicalSequence analysisAdd BLAST21
Transmembranei303 – 323HelicalSequence analysisAdd BLAST21
Transmembranei342 – 362HelicalSequence analysisAdd BLAST21
Transmembranei385 – 405HelicalSequence analysisAdd BLAST21
Transmembranei519 – 539HelicalSequence analysisAdd BLAST21
Transmembranei551 – 571HelicalSequence analysisAdd BLAST21

Keywords - Cellular componenti

Cell membrane, Membrane

Pathology & Biotechi

Disruption phenotypei

Fishes display the backstroke phenotype. Mutant fish exhibit otolith agenesis without additional effects on the sensory epithelium or other inner ear structures. Otoliths are essential for the perception of gravity and acceleration. Larvae without otoliths are unable to orient dorsal side up and rest mostly on their side in contact with the ground.3 Publications

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi429E → V in backstroke; causes otholith agenesis. 1 Publication1

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00003138191 – 586Proton channel OTOP1Add BLAST586

Proteomic databases

PRIDEiQ7ZWK8

Expressioni

Developmental stagei

First detected in the otic placode at 16.5 hours post-fertilization (hpf). Detected in the developing inner ear sensory epithelium at 24 hpf. Detected in hair cells of the sensory epithelium at 4 days post-fertilization (dpf). At 5 dpf, levels are much reduced in the otolith organs, and not detectable at 7 dpf. Detected in neuromasts and in the lateral line system at 5 dpf and 7 dpf.2 Publications

Family & Domainsi

Sequence similaritiesi

Belongs to the otopetrin family.Curated

Keywords - Domaini

Transmembrane, Transmembrane helix

Phylogenomic databases

HOVERGENiHBG108223
InParanoidiQ7ZWK8
PhylomeDBiQ7ZWK8

Family and domain databases

InterProiView protein in InterPro
IPR004878 Otopetrin
PANTHERiPTHR21522 PTHR21522, 1 hit
PfamiView protein in Pfam
PF03189 Otopetrin, 3 hits

Sequencei

Sequence statusi: Complete.

Q7ZWK8-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MVEHGGTDSM WLNKYNPAAA SSASSSSSSD AENKLFSRLK VSLTKKYPQK
60 70 80 90 100
NAELLSAQYG TNLLLLGVSV MLALAAQSGP VKEEHLLSFI TVLMLVQLVW
110 120 130 140 150
MLCYMIRRER ERSPVPERDA HAGASWIRGG LTMLALLSLI MDAFRIGYFV
160 170 180 190 200
GYHSCISAAL GVYPIVHALH TISQVHFLWF HIKDVIKKYE TFERFGVIHA
210 220 230 240 250
VFTNLLLWCN GVMSETEHFM HNHRRRLIEM GYANLSTVDV QPHCNCTTSV
260 270 280 290 300
CSMFSTSLYY LYPFNIEYHI FVSAMLFVMW KNIGRTLDRH SNRKRRSTGS
310 320 330 340 350
TGLLLGPLGG LVALASSVSV LVVYLIHLEK TEEMHEAAVS MFYYYGVAMM
360 370 380 390 400
ACMCVGSGTG LLVYRMENRP MDTGSNPART LDTELLLASS LGSWLMSWCS
410 420 430 440 450
VVASVAEAGQ KSPSFSWTSL TYSLLLVLEK CIQNLFIVES LYRRHSEEEE
460 470 480 490 500
DAAAPQVFSV AVPPYDGILN HGYEAHDKHR EAEPAAGSHA LSRKQPDAPL
510 520 530 540 550
PAGQRLDVTP GRKRQILKNI CMFLFMCNIS LWILPAFGCR PQYDNPLENE
560 570 580
TFGTSVWTTV LNVAIPLNLF YRMHSVASLF EVFRKV
Length:586
Mass (Da):65,721
Last modified:January 15, 2008 - v2
Checksum:i000ED7A4E690831E
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti171T → A in AAH83445 (Ref. 3) Curated1
Sequence conflicti171T → A in AAI64660 (Ref. 3) Curated1
Sequence conflicti251 – 252CS → WL in AAN62756 (PubMed:12651873).Curated2
Sequence conflicti300S → T in AAH83445 (Ref. 3) Curated1
Sequence conflicti300S → T in AAI64660 (Ref. 3) Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AY170648 mRNA Translation: AAN62756.1
AY744157 mRNA Translation: AAV91780.1
BC083445 mRNA Translation: AAH83445.1
BC164660 mRNA Translation: AAI64660.1
RefSeqiNP_942098.1, NM_198803.1
UniGeneiDr.77257

Genome annotation databases

GeneIDi322893
KEGGidre:322893

Similar proteinsi

Entry informationi

Entry nameiOTOP1_DANRE
AccessioniPrimary (citable) accession number: Q7ZWK8
Secondary accession number(s): B2GQA4, Q5MKL8, Q5XJ61
Entry historyiIntegrated into UniProtKB/Swiss-Prot: January 15, 2008
Last sequence update: January 15, 2008
Last modified: May 23, 2018
This is version 67 of the entry and version 2 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

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