Reviewed, UniProtKB/Swiss-Prot Q7ZWK8 (OTOP1_DANRE)

Names and origin

Protein names

Recommended name:
    Otopetrin-1

Gene names

Name:

otop1

Organism

Danio rerio (Zebrafish) (Brachydanio rerio)

Taxonomic identifier

7955 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Actinopterygii › Neopterygii › Teleostei › Ostariophysi › Cypriniformes › Cyprinidae › Danio

Protein attributes

Sequence length	586 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at protein level.

General annotation (Comments)

Function	Inhibits P2Y purinoceptors. Modulates calcium homeostasis and influx of calcium in response to extracellular ATP By similarity. Essential for the formation of otoliths in the inner ear of developing larvae and for the perception of gravity and acceleration.
Subcellular location	Membrane; Multi-pass membrane protein. Secreted › extracellular spaceBy similarity.
Developmental stage	First detected in the otic placode at 16.5 hours post-fertilization (hpf). Detected in the developing inner ear sensory epithelium at 24 hpf. Detected in hair cells of the sensory epithelium at 4 days post-fertilization (dpf). At 5 dpf, levels are much reduced in the otolith organs, and not detectable at 7 dpf. Detected in neuromasts and in the lateral line system at 5 dpf and 7 dpf.
Miscellaneous	Fishes lacking otop1 display the backstroke phenotype. Mutant fish exhibit otolith agenesis without additional effects on the sensory epithelium or other inner ear structures. Otoliths are essential for the perception of gravity and acceleration. Larvae without otoliths are unable to orient dorsal side up and rest mostly on their side in contact with the ground. Otoliths are structures composed of proteins and calcium carbonate that are found in the fish inner ear in contact with the otic epithelium. They are essential for the perception of gravity and acceleration.
Sequence similarities	Belongs to the otopetrin family.

Ontologies

Keywords
Biological process	Biomineralization
Cellular component	Membrane Secreted
Domain	Transmembrane
Gene Ontology (GO)
Biological process	inner ear morphogenesis Ref.2 Inferred from mutant phenotype. Source: ZFIN ossification Inferred from electronic annotation. Source: UniProtKB-KW otolith mineralization Ref.2 Ref.4 Inferred from mutant phenotype. Source: ZFIN
Cellular component	extracellular region Inferred from electronic annotation. Source: UniProtKB-KW integral to membrane Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 586	586	Otopetrin-1		PRO_0000313819
Regions
	Transmembrane	54 – 74	21	Potential
	Transmembrane	86 – 106	21	Potential
	Transmembrane	124 – 144	21	Potential
	Transmembrane	146 – 166	21	Potential
	Transmembrane	195 – 215	21	Potential
	Transmembrane	260 – 280	21	Potential
	Transmembrane	303 – 323	21	Potential
	Transmembrane	342 – 362	21	Potential
	Transmembrane	519 – 539	21	Potential
	Transmembrane	551 – 571	21	Potential
Experimental info
	Mutagenesis	429	1	E → V in backstroke; causes otholith agenesis
	Sequence conflict	171	1	T → A in AAH83445. Ref.3
	Sequence conflict	251 – 252	2	CS → WL in AAN62756. Ref.1
	Sequence conflict	300	1	S → T in AAH83445. Ref.3

Sequences

	Sequence		Length	Mass (Da)
	Q7ZWK8-1 [UniParc]. Last modified January 15, 2008. Version 2. Checksum: 000ED7A4E690831E	FASTA	586	65,721
	10 20 30 40 50 60 MVEHGGTDSM WLNKYNPAAA SSASSSSSSD AENKLFSRLK VSLTKKYPQK NAELLSAQYG 70 80 90 100 110 120 TNLLLLGVSV MLALAAQSGP VKEEHLLSFI TVLMLVQLVW MLCYMIRRER ERSPVPERDA 130 140 150 160 170 180 HAGASWIRGG LTMLALLSLI MDAFRIGYFV GYHSCISAAL GVYPIVHALH TISQVHFLWF 190 200 210 220 230 240 HIKDVIKKYE TFERFGVIHA VFTNLLLWCN GVMSETEHFM HNHRRRLIEM GYANLSTVDV 250 260 270 280 290 300 QPHCNCTTSV CSMFSTSLYY LYPFNIEYHI FVSAMLFVMW KNIGRTLDRH SNRKRRSTGS 310 320 330 340 350 360 TGLLLGPLGG LVALASSVSV LVVYLIHLEK TEEMHEAAVS MFYYYGVAMM ACMCVGSGTG 370 380 390 400 410 420 LLVYRMENRP MDTGSNPART LDTELLLASS LGSWLMSWCS VVASVAEAGQ KSPSFSWTSL 430 440 450 460 470 480 TYSLLLVLEK CIQNLFIVES LYRRHSEEEE DAAAPQVFSV AVPPYDGILN HGYEAHDKHR 490 500 510 520 530 540 EAEPAAGSHA LSRKQPDAPL PAGQRLDVTP GRKRQILKNI CMFLFMCNIS LWILPAFGCR 550 560 570 580 PQYDNPLENE TFGTSVWTTV LNVAIPLNLF YRMHSVASLF EVFRKV

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Non-syndromic vestibular disorder with otoconial agenesis in tilted/mergulhador mice caused by mutations in otopetrin 1." Hurle B., Ignatova E., Massironi S.M., Mashimo T., Rios X., Thalmann I., Thalmann R., Ornitz D.M. Hum. Mol. Genet. 12:777-789(2003) [PubMed: 12651873] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA]. Tissue: Embryo.
[2]	"Mutated otopetrin 1 affects the genesis of otoliths and the localization of Starmaker in zebrafish." Soellner C., Schwarz H., Geisler R., Nicolson T. Dev. Genes Evol. 214:582-590(2004) [PubMed: 15480759] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA], FUNCTION, DEVELOPMENTAL STAGE, SUBCELLULAR LOCATION, CHARACTERIZATION OF MUTANT VAL-429.
[3]	NIH - Zebrafish Gene Collection (ZGC) project Submitted (OCT-2004) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Tissue: Larva.
[4]	"Otopetrin 1 is required for otolith formation in the zebrafish Danio rerio." Hughes I., Blasiole B., Huss D., Warchol M.E., Rath N.P., Hurle B., Ignatova E., Dickman J.D., Thalmann R., Levenson R., Ornitz D.M. Dev. Biol. 276:391-402(2004) [PubMed: 15581873] [Abstract] Cited for: FUNCTION, DISEASE, DEVELOPMENTAL STAGE.

Web resources

Protein Spotlight

Ear of stone - Issue 89 of December 2007

Cross-references

Sequence databases
EMBLGenBankDDBJ	AY170648 mRNA. Translation: AAN62756.1. AY744157 mRNA. Translation: AAV91780.1. BC083445 mRNA. Translation: AAH83445.1.
RefSeq	NP_942098.1.
UniGene	Dr.77257
3D structure databases
ModBase	Search...
Genome annotation databases
Ensembl	ENSDARG00000021566. Danio rerio. [Contig view]
GeneID	322893.
KEGG	dre:322893.
Organism-specific databases
ZFIN	ZDB-GENE-030131-1613. otop1.
Phylogenomic databases
HOVERGEN	Q7ZWK8.
Family and domain databases
InterPro	IPR004878. DUF270. [Graphical view]
PANTHER	PTHR21522. DUF270. 1 hit.
ProtoNet	Search...

Entry information

Entry name

OTOP1_DANRE

Accession

Primary (citable) accession number: Q7ZWK8
Secondary accession number(s): Q5MKL8, Q5XJ61

Entry history

Integrated into UniProtKB/Swiss-Prot:	January 15, 2008
Last sequence update:	January 15, 2008
Last modified:	November 25, 2008
This is version 22 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

Zebrafish annotation project