Reviewed, UniProtKB/Swiss-Prot Q7ZW77 (ERG25_DANRE)

Names and origin

Protein names

Recommended name:
    C-4 methylsterol oxidase
    EC=1.14.13.72
Alternative name(s):
    Methylsterol monooxygenase

Gene names

Name:	sc4mol
ORF Names:	zgc:56437

Organism

Danio rerio (Zebrafish) (Brachydanio rerio)

Taxonomic identifier

7955 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Actinopterygii › Neopterygii › Teleostei › Ostariophysi › Cypriniformes › Cyprinidae › Danio

Protein attributes

Sequence length	291 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at transcript level.

General annotation (Comments)

Catalytic activity	4,4-dimethyl-5-alpha-cholest-7-en-3-beta-ol + NAD(P)H + O2 = 4-beta-hydroxymethyl-4-alpha-methyl-5-alpha-cholest-7-en-3-beta-ol + NAD(P)+ + H2O. 4-beta-hydroxymethyl-4-alpha-methyl-5-alpha-cholest-7-en-3-beta-ol + NAD(P)H + O2 = 3-beta-hydroxy-4-beta-methyl-5-alpha-cholest-7-ene-4-alpha-carbaldehyde + NAD(P)+ + 2 H2O. 3-beta-hydroxy-4-beta-methyl-5-alpha-cholest-7-ene-4-alpha-carbaldehyde + NAD(P)H + O2 = 3-beta-hydroxy-4-beta-methyl-5-alpha-cholest-7-ene-4-alpha-carboxylate + NAD(P)+ + H2O.
Cofactor	Iron By similarity.
Pathway	Steroid biosynthesis; zymosterol biosynthesis; zymosterol from lanosterol: step 3/6.
Subcellular location	Endoplasmic reticulum membrane; Multi-pass membrane protein Probable.
Domain	The histidine box domains may contain the active site and/or be involved in metal ion binding.
Sequence similarities	Belongs to the sterol desaturase family.

Ontologies

Keywords
Biological process	Lipid synthesis Steroid biosynthesis Sterol biosynthesis
Cellular component	Endoplasmic reticulum Membrane
Domain	Transmembrane
Ligand	Iron NAD
Molecular function	Oxidoreductase
Gene Ontology (GO)
Biological process	fatty acid biosynthetic process Inferred from electronic annotation. Source: InterPro oxidation reduction Inferred from electronic annotation. Source: UniProtKB-KW sterol biosynthetic process Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	endoplasmic reticulum Inferred from electronic annotation. Source: UniProtKB-KW integral to membrane Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	C-4 methylsterol oxidase activity Inferred from electronic annotation. Source: EC iron ion binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 291	291	C-4 methylsterol oxidase		PRO_0000249853
Regions
	Transmembrane	55 – 75	21	Potential
	Transmembrane	100 – 120	21	Potential
	Transmembrane	199 – 219	21	Potential
	Motif	157 – 161	5	Histidine box-1 By similarity
	Motif	170 – 174	5	Histidine box-2 By similarity
	Motif	249 – 255	7	Histidine box-3 By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q7ZW77-1 [UniParc]. Last modified June 1, 2003. Version 1. Checksum: 67A435866F99E116	FASTA	291	34,807
	10 20 30 40 50 60 MEVNGTANIL SSAFLAVEFV DSFLPQNPLQ EPFKHAWNHM LQNYTKFQIA TWGSLIVHEL 70 80 90 100 110 120 IYFLFCLPGF IFQFLPFMQK YKIQPDKPET WEKQWKCFKM LLFNHFCIQL PLICGTYYFT 130 140 150 160 170 180 EFFSIPYDWD TMPRWPFLLA QCFGCAVIED TWHYFLHRAL HHRRIYKYIH KVHHDFTSPF 190 200 210 220 230 240 GMQAEYAHPL ETLILGAGFF IGTMVFCNHM ILLWAWVTFR LLETIDVHSG YDIPLNPLHL 250 260 270 280 290 IPFYAGARFH DFHHMNFVGN YGSTFTWWDR LFDTDSQFNK HYSHHKTAKS D

References

[1]	NIH - Zebrafish Gene Collection (ZGC) project Submitted (APR-2003) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Strain: SJD.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBLGenBankDDBJ	BC050163 mRNA. Translation: AAH50163.1.
IPI	IPI00503385.
RefSeq	NP_998518.1.
UniGene	Dr.12110
3D structure databases
ModBase	Search...
Protein-protein interaction databases
STRING	Q7ZW77.
Genome annotation databases
Ensembl	ENSDART00000078277; ENSDARP00000072739; ENSDARG00000055876; Danio rerio. [Genome view]
GeneID	406662.
KEGG	dre:406662.
Organism-specific databases
CTD	406662.
ZFIN	ZDB-GENE-040426-2670. sc4mol.
Phylogenomic databases
HOVERGEN	Q7ZW77.
Enzyme and pathway databases
BRENDA	1.14.13.72. 96826.
Gene expression databases
ArrayExpress	Q7ZW77.
Bgee	Q7ZW77.
Family and domain databases
InterPro	IPR006694. Fatty_acid_hydroxylase. [Graphical view]
Pfam	PF04116. FA_hydroxylase. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

ERG25_DANRE

Accession

Primary (citable) accession number: Q7ZW77

Entry history

Integrated into UniProtKB/Swiss-Prot:	September 19, 2006
Last sequence update:	June 1, 2003
Last modified:	October 13, 2009
This is version 44 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

Zebrafish annotation project