Reviewed, UniProtKB/Swiss-Prot Q7ZW38 (SPS1_DANRE)

Names and origin

Protein names

Recommended name:
    Selenide, water dikinase 1
    EC=2.7.9.3
Alternative name(s):
    Selenophosphate synthetase 1
    Selenium donor protein 1

Gene names

Name:	sephs1
ORF Names:	si:ch211-220f12.5

Organism

Danio rerio (Zebrafish) (Brachydanio rerio)

Taxonomic identifier

7955 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Actinopterygii › Neopterygii › Teleostei › Ostariophysi › Cypriniformes › Cyprinidae › Danio

Protein attributes

Sequence length	392 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at protein level.

General annotation (Comments)

Function	Synthesizes selenophosphate from selenide and ATP By similarity.
Catalytic activity	ATP + selenide + H2O = AMP + selenophosphate + phosphate.
Sequence similarities	Belongs to the selenophosphate synthetase 1 family. Class II subfamily.
Caution	The conserved active site Cys (or selenocysteine) residue in position 29 is replaced by a Thr. However, as function in selenoprotein synthesis is probable, it is possible Cys-31 is the active site.

Ontologies

Keywords
Ligand	ATP-binding Nucleotide-binding Selenium
Molecular function	Kinase Transferase
PTM	Phosphoprotein
Gene Ontology (GO)
Molecular function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW selenide, water dikinase activity Inferred from electronic annotation. Source: EC selenium binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 392	392	Selenide, water dikinase 1		PRO_0000312508
Regions
	Nucleotide binding	268 – 274	7	ATP Potential
Sites
	Active site	31	1	Potential
	Site	32	1	Important for catalytic activity By similarity
Amino acid modifications
	Modified residue	387	1	Phosphothreonine Ref.3
Experimental info
	Sequence conflict	138	1	D → G in AAH45302. Ref.2

Sequences

	Sequence		Length	Mass (Da)
	Q7ZW38-1 [UniParc]. Last modified December 4, 2007. Version 2. Checksum: 5DF8079CC9317B6E	FASTA	392	42,923
	10 20 30 40 50 60 MSVRESFNPE SYELDKNFRL TRFTELKGTG CKVPQDVLQK LLESLQENHY QEDEQFLGAV 70 80 90 100 110 120 MPRLGIGMDT CVIPLRHGGL SLVQTTDYIY PIVDDPYMMG RIACANVLSD LYAMGVTECD 130 140 150 160 170 180 NMLMLLGVSN KLTEKERDKV MPLVIQGFKD ASEEAGTSVT GGQTVINPWI VLGGVATTVC 190 200 210 220 230 240 QPNEFIMPDN AVPGDVLVLT KPLGTQVAVA VHQWLDIPEK WNKIKLVVTQ EDVELAYQEA 250 260 270 280 290 300 MLNMARLNRT AAGLMHTFNA HAATDITGFG ILGHAQNLAR QQRTEVSFVI HNLPVLAKMA 310 320 330 340 350 360 AVSKACGNMF GLMHGTCPET SGGLLICLPR EQAARFCAEI KSPKYGEGHQ AWIIGIVEKG 370 380 390 NRTARIIDKP RIIEVAPQVA TQNVNTTPGA TS

References

[1]	The Danio rerio sequencing project at the Sanger Institute Submitted (JUN-2007) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: Tuebingen.
[2]	NIH - Zebrafish Gene Collection (ZGC) project Submitted (JAN-2003) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Strain: AB. Tissue: Embryo.
[3]	"Online automated in vivo zebrafish phosphoproteomics: from large-scale analysis down to a single embryo." Lemeer S., Pinkse M.W.H., Mohammed S., van Breukelen B., den Hertog J., Slijper M., Heck A.J.R. J. Proteome Res. 7:1555-1564(2008) [PubMed: 18307296] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT THR-387, MASS SPECTROMETRY. Tissue: Embryo.

Cross-references

Sequence databases
EMBLGenBankDDBJ	BX276107, BX248392 Genomic DNA. Translation: CAK04523.1. BX248392, BX276107 Genomic DNA. Translation: CAM56407.1. BC045302 mRNA. Translation: AAH45302.1.
IPI	IPI00499953.
RefSeq	NP_955995.2.
UniGene	Dr.20919
3D structure databases
ModBase	Search...
Genome annotation databases
Ensembl	ENSDART00000081143; ENSDARP00000075586; ENSDARG00000058292; Danio rerio. [Genome view]
GeneID	324947.
KEGG	dre:324947.
NMPDR	fig|7955.3.peg.8739.
Organism-specific databases
CTD	324947.
ZFIN	ZDB-GENE-030131-3670. sephs1.
Phylogenomic databases
HOVERGEN	Q7ZW38.
OMA	MMAIAIL.
Enzyme and pathway databases
BRENDA	2.7.9.3. 96826.
Gene expression databases
ArrayExpress	Q7ZW38.
Bgee	Q7ZW38.
Family and domain databases
InterPro	IPR000728. AIR_synth. IPR010918. AIR_synth_C. IPR004536. SelD. [Graphical view]
Pfam	PF00586. AIRS. 1 hit. PF02769. AIRS_C. 1 hit. [Graphical view]
PIRSF	PIRSF036407. Selenphspht_syn. 1 hit.
TIGRFAMs	TIGR00476. selD. 1 hit.
ProtoNet	Search...

Entry information

Entry name

SPS1_DANRE

Accession

Primary (citable) accession number: Q7ZW38
Secondary accession number(s): Q1LXT8

Entry history

Integrated into UniProtKB/Swiss-Prot:	December 4, 2007
Last sequence update:	December 4, 2007
Last modified:	November 3, 2009
This is version 46 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

Zebrafish annotation project