TFIIH basal transcription factor complex helicase XPB subunit

Preferred order of sections

Names and origin

Protein names

Recommended name:
TFIIH basal transcription factor complex helicase XPB subunit
EC=3.6.4.12

Alternative name(s):
DNA excision repair protein ERCC-3

Gene names

Name:

ercc3

Organism

Danio rerio (Zebrafish) (Brachydanio rerio) [Reference proteome]

Taxonomic identifier

7955 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Actinopterygii › Neopterygii › Teleostei › Ostariophysi › Cypriniformes › Cyprinidae › Danio

Protein attributes

Sequence length	782 AA.
Sequence status	Complete.
Protein existence	Evidence at transcript level

General annotation (Comments)

Function	ATP-dependent 3'-5' DNA helicase, component of the core-TFIIH basal transcription factor, involved in nucleotide excision repair (NER) of DNA and, when complexed to CAK, in RNA transcription by RNA polymerase II. Acts by opening DNA either around the RNA transcription start site or the DNA damage By similarity.
Catalytic activity	ATP + H₂O = ADP + phosphate.
Subunit structure	One of the six subunits forming the core-TFIIH basal transcription factor which associates with the CAK complex composed of CDK7, CCNH/cyclin H and MNAT1 to form the TFIIH basal transcription factor By similarity.
Subcellular location	Nucleus By similarity.
Sequence similarities	Belongs to the helicase family. RAD25/XPB subfamily. Contains 1 helicase ATP-binding domain. Contains 1 helicase C-terminal domain.

Ontologies

Keywords
Biological process	DNA damage DNA repair Transcription Transcription regulation
Cellular component	Nucleus
Ligand	ATP-binding DNA-binding Nucleotide-binding
Molecular function	Helicase Hydrolase
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	DNA topological change Inferred from sequence or structural similarity. Source: UniProtKB induction of apoptosis Inferred from sequence or structural similarity. Source: UniProtKB protein phosphorylation Inferred from sequence or structural similarity. Source: GOC regulation of transcription, DNA-dependent Inferred from electronic annotation. Source: UniProtKB-KW transcription from RNA polymerase II promoter Inferred from sequence or structural similarity. Source: UniProtKB transcription-coupled nucleotide-excision repair Inferred from sequence or structural similarity. Source: UniProtKB
Cellular_component	core TFIIH complex Inferred from electronic annotation. Source: InterPro holo TFIIH complex Inferred from sequence or structural similarity. Source: UniProtKB
Molecular_function	3'-5' DNA helicase activity Inferred from sequence or structural similarity. Source: UniProtKB ATP binding Inferred from electronic annotation. Source: UniProtKB-KW ATP-dependent DNA helicase activity Inferred from electronic annotation. Source: InterPro DNA binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Chain

1 – 782

782

TFIIH basal transcription factor complex helicase XPB subunit

PRO_0000323744

Regions

Domain

326 – 487

162

Helicase ATP-binding

Domain

541 – 701

161

Helicase C-terminal

Nucleotide binding

339 – 346

8

ATP By similarity

Motif

6 – 17

12

Nuclear localization signal Potential

Motif

440 – 443

4

DEVH box

Compositional bias

19 – 26

8

Asp/Glu-rich (acidic)

Compositional bias

257 – 263

7

Asp/Glu-rich (acidic)

Compositional bias

696 – 699

4

Asp/Glu-rich (acidic)

Compositional bias

720 – 726

7

Asp/Glu-rich (acidic)

Sequences

Sequence

Length

Mass (Da)

Tools

Q7ZVV1 [UniParc].

Last modified June 1, 2003. Version 1.
Checksum: 5CAA94DA15CA7A15
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FASTA

782

89,288

        10         20         30         40         50         60 
MGRKDKSDRE KKSKKRYYED EEEDEEVIGG ESQEAVPAAA GKQVDESSTK LDEYGAKDYR 

        70         80         90        100        110        120 
LQMLLKNDHS SRPLWVAPDG HIFLEAFSPV YKYAQDFLVA ISEPVCRPTH AHEYKLTAYS 

       130        140        150        160        170        180 
LYAAVSVGLQ TSDIIEYLQK LSKTSVPDGI VQFIKLCTVS YGKVKLVLKH NRYFVESAFP 

       190        200        210        220        230        240 
DVIQRLLQDT VIRDCRLRSA EGEETELITE TISSKSAISK SQQDNGGPSS SQPADGQRSG 

       250        260        270        280        290        300 
TQVPEDIFSY YEQMDKEEEE EEETQTVSFE IRQEMIEELQ KRCIQLEYPL LAEYDFRNDT 

       310        320        330        340        350        360 
VNPDINMDLK PTAVLRPYQE KSLRKMFGNG RARSGVIVLP CGAGKSLVGV TAACTVRKRC 

       370        380        390        400        410        420 
LVLGNSSVSV EQWKAQFKMW STIDDSQICR FTSDAKDKPI GCSVAISTYS MLGHTTKRSW 

       430        440        450        460        470        480 
EAERVMEWMK SQEWGLIILD EVHTIPAKMF RRVLTIVQAH CKLGLTATLV REDDKIVDLN 

       490        500        510        520        530        540 
FLIGPKLYEA NWMELQNNGY IAKVQCAEVW CPMSPEFYRE YVAIKTKKRI LLYTMNPNKF 

       550        560        570        580        590        600 
RACQFLIRFH ERRNDKIIVF ADNVFALKEY AIRLNKPYIY GPTSQGERMQ ILQNFKHNPK 

       610        620        630        640        650        660 
INTIFISKVG DTSFDLPEAN VLIQISSHGG SRRQEAQRLG RVLRAKKGMV AEEYNAYFYS 

       670        680        690        700        710        720 
LVSQDTQEMA YSTKRQRFLV DQGYSFKVIT KLAGMEEEDL MFSTRDEQQQ LLQKVLAASD 

       730        740        750        760        770        780 
LDAEEEVVMG EVGGKPQFSR RAGTMSSMSG ADDALYMEYQ MPRGSKASVG KNIHPLFKRF 


RK

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References

[1]	NIH - Zebrafish Gene Collection (ZGC) project Submitted (JAN-2003) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Strain: AB.

Cross-references

Sequence databases
EMBL GenBank DDBJ	BC045400 mRNA. Translation: AAH45400.1.
IPI	IPI00509371.
RefSeq	NP_963876.1. NM_201582.1.
UniGene	Dr.6574.
3D structure databases
HSSP	HSSP built from PDB template 2FWR based on UniProtKB O29889.
ProteinModelPortal	Q7ZVV1.
ModBase	Search...
Protein-protein interaction databases
STRING	7955.ENSDARP00000010637.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	324323.
KEGG	dre:324323.
Organism-specific databases
CTD	2071.
ZFIN	ZDB-GENE-030131-3043. ercc3.
Phylogenomic databases
eggNOG	COG1061.
HOGENOM	HOG000160172.
HOVERGEN	HBG051499.
InParanoid	Q7ZVV1.
KO	K10843.
OrthoDB	EOG4KD6KG.
Family and domain databases
InterPro	IPR006935. Helicase/UvrB_dom. IPR014001. Helicase_ATP-bd. IPR001650. Helicase_C. IPR001161. Helicase_Ercc3. [Graphical view]
Pfam	PF00271. Helicase_C. 1 hit. PF04851. ResIII. 1 hit. [Graphical view]
PRINTS	PR00851. XRODRMPGMNTB.
SMART	SM00487. DEXDc. 1 hit. SM00490. HELICc. 1 hit. [Graphical view]
TIGRFAMs	TIGR00603. rad25. 1 hit.
PROSITE	PS51192. HELICASE_ATP_BIND_1. 1 hit. PS51194. HELICASE_CTER. 1 hit. [Graphical view]
ProtoNet	Search...
Other
NextBio	20808715.

Entry information

Entry name

ERCC3_DANRE

Accession

Primary (citable) accession number: Q7ZVV1

Entry history

Integrated into UniProtKB/Swiss-Prot:	March 18, 2008
Last sequence update:	June 1, 2003
Last modified:	May 1, 2013
This is version 85 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

Preferred order of sections

Names and origin

Protein attributes

General annotation (Comments)

Ontologies

Sequence annotation (Features)

Molecule processing

Regions

Sequences

References

Cross-references

Sequence databases

3D structure databases

Protein-protein interaction databases

Protocols and materials databases

Genome annotation databases

Organism-specific databases

Phylogenomic databases

Family and domain databases

Other

Entry information

Relevant documents