ID   Q7ZVL7_DANRE            Unreviewed;       919 AA.
AC   Q7ZVL7;
DT   01-JUN-2003, integrated into UniProtKB/TrEMBL.
DT   01-JUN-2003, sequence version 1.
DT   27-MAR-2024, entry version 122.
DE   RecName: Full=Hexokinase-2 {ECO:0000256|ARBA:ARBA00039453};
DE            EC=2.7.1.1 {ECO:0000256|ARBA:ARBA00012324};
DE   AltName: Full=Hexokinase type II {ECO:0000256|ARBA:ARBA00041371};
GN   Name=hk2 {ECO:0000313|EMBL:AAH45496.1, ECO:0000313|RefSeq:NP_998231.1,
GN   ECO:0000313|ZFIN:ZDB-GENE-040426-2017};
GN   Synonyms=fi09d05 {ECO:0000313|RefSeq:NP_998231.1}, wu:fi09d05
GN   {ECO:0000313|RefSeq:NP_998231.1}, wu:fi17h06
GN   {ECO:0000313|RefSeq:NP_998231.1}, wu:fi31b04
GN   {ECO:0000313|RefSeq:NP_998231.1}, zgc:55926
GN   {ECO:0000313|RefSeq:NP_998231.1};
OS   Danio rerio (Zebrafish) (Brachydanio rerio).
OC   Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
OC   Actinopterygii; Neopterygii; Teleostei; Ostariophysi; Cypriniformes;
OC   Danionidae; Danioninae; Danio.
OX   NCBI_TaxID=7955 {ECO:0000313|EMBL:AAH45496.1};
RN   [1] {ECO:0000313|EMBL:AAH45496.1}
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC   STRAIN=AB {ECO:0000313|EMBL:AAH45496.1};
RC   TISSUE=Whole body {ECO:0000313|EMBL:AAH45496.1};
RG   NIH - Zebrafish Gene Collection (ZGC) project;
RL   Submitted (JAN-2003) to the EMBL/GenBank/DDBJ databases.
RN   [2] {ECO:0000313|RefSeq:NP_998231.1}
RP   NUCLEOTIDE SEQUENCE.
RC   STRAIN=AB {ECO:0000313|RefSeq:NP_998231.1};
RX   PubMed=16109975;
RA   Woods I.G., Wilson C., Friedlander B., Chang P., Reyes D.K., Nix R.,
RA   Kelly P.D., Chu F., Postlethwait J.H., Talbot W.S.;
RT   "The zebrafish gene map defines ancestral vertebrate chromosomes.";
RL   Genome Res. 15:1307-1314(2005).
RN   [3] {ECO:0000313|RefSeq:NP_998231.1}
RP   NUCLEOTIDE SEQUENCE.
RC   STRAIN=AB {ECO:0000313|RefSeq:NP_998231.1};
RX   PubMed=17013923; DOI=10.1002/neu.20328;
RA   Harden M.V., Newton L.A., Lloyd R.C., Whitlock K.E.;
RT   "Olfactory imprinting is correlated with changes in gene expression in the
RT   olfactory epithelia of the zebrafish.";
RL   J. Neurobiol. 66:1452-1466(2006).
RN   [4] {ECO:0000313|RefSeq:NP_998231.1}
RP   NUCLEOTIDE SEQUENCE.
RC   STRAIN=AB {ECO:0000313|RefSeq:NP_998231.1};
RX   PubMed=19087890;
RA   Gonzalez-Alvarez R., Ortega-Cuellar D., Hernandez-Mendoza A.,
RA   Moreno-Arriola E., Villasenor-Mendoza K., Galvez-Mariscal A.,
RA   Perez-Cruz M.E., Morales-Salas I., Velazquez-Arellano A.;
RT   "The hexokinase gene family in the zebrafish: structure, expression,
RT   functional and phylogenetic analysis.";
RL   Comp. Biochem. Physiol. B, Biochem. Mol. Biol. 152:189-195(2009).
RN   [5] {ECO:0000313|RefSeq:NP_998231.1}
RP   NUCLEOTIDE SEQUENCE.
RC   STRAIN=AB {ECO:0000313|RefSeq:NP_998231.1};
RX   PubMed=23341543;
RA   Harris J.M., Esain V., Frechette G.M., Harris L.J., Cox A.G., Cortes M.,
RA   Garnaas M.K., Carroll K.J., Cutting C.C., Khan T., Elks P.M., Renshaw S.A.,
RA   Dickinson B.C., Chang C.J., Murphy M.P., Paw B.H., Vander Heiden M.G.,
RA   Goessling W., North T.E.;
RT   "Glucose metabolism impacts the spatiotemporal onset and magnitude of HSC
RT   induction in vivo.";
RL   Blood 121:2483-2493(2013).
RN   [6] {ECO:0000313|Proteomes:UP000000437}
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RX   PubMed=23594743; DOI=10.1038/nature12111;
RG   Genome Reference Consortium Zebrafish;
RA   Howe K., Clark M.D., Torroja C.F., Torrance J., Berthelot C., Muffato M.,
RA   Collins J.E., Humphray S., McLaren K., Matthews L., McLaren S., Sealy I.,
RA   Caccamo M., Churcher C., Scott C., Barrett J.C., Koch R., Rauch G.J.,
RA   White S., Chow W., Kilian B., Quintais L.T., Guerra-Assuncao J.A., Zhou Y.,
RA   Gu Y., Yen J., Vogel J.H., Eyre T., Redmond S., Banerjee R., Chi J., Fu B.,
RA   Langley E., Maguire S.F., Laird G.K., Lloyd D., Kenyon E., Donaldson S.,
RA   Sehra H., Almeida-King J., Loveland J., Trevanion S., Jones M., Quail M.,
RA   Willey D., Hunt A., Burton J., Sims S., McLay K., Plumb B., Davis J.,
RA   Clee C., Oliver K., Clark R., Riddle C., Elliot D., Eliott D.,
RA   Threadgold G., Harden G., Ware D., Begum S., Mortimore B., Mortimer B.,
RA   Kerry G., Heath P., Phillimore B., Tracey A., Corby N., Dunn M.,
RA   Johnson C., Wood J., Clark S., Pelan S., Griffiths G., Smith M.,
RA   Glithero R., Howden P., Barker N., Lloyd C., Stevens C., Harley J.,
RA   Holt K., Panagiotidis G., Lovell J., Beasley H., Henderson C., Gordon D.,
RA   Auger K., Wright D., Collins J., Raisen C., Dyer L., Leung K.,
RA   Robertson L., Ambridge K., Leongamornlert D., McGuire S., Gilderthorp R.,
RA   Griffiths C., Manthravadi D., Nichol S., Barker G., Whitehead S., Kay M.,
RA   Brown J., Murnane C., Gray E., Humphries M., Sycamore N., Barker D.,
RA   Saunders D., Wallis J., Babbage A., Hammond S., Mashreghi-Mohammadi M.,
RA   Barr L., Martin S., Wray P., Ellington A., Matthews N., Ellwood M.,
RA   Woodmansey R., Clark G., Cooper J., Cooper J., Tromans A., Grafham D.,
RA   Skuce C., Pandian R., Andrews R., Harrison E., Kimberley A., Garnett J.,
RA   Fosker N., Hall R., Garner P., Kelly D., Bird C., Palmer S., Gehring I.,
RA   Berger A., Dooley C.M., Ersan-Urun Z., Eser C., Geiger H., Geisler M.,
RA   Karotki L., Kirn A., Konantz J., Konantz M., Oberlander M.,
RA   Rudolph-Geiger S., Teucke M., Lanz C., Raddatz G., Osoegawa K., Zhu B.,
RA   Rapp A., Widaa S., Langford C., Yang F., Schuster S.C., Carter N.P.,
RA   Harrow J., Ning Z., Herrero J., Searle S.M., Enright A., Geisler R.,
RA   Plasterk R.H., Lee C., Westerfield M., de Jong P.J., Zon L.I.,
RA   Postlethwait J.H., Nusslein-Volhard C., Hubbard T.J., Roest Crollius H.,
RA   Rogers J., Stemple D.L.;
RT   "The zebrafish reference genome sequence and its relationship to the human
RT   genome.";
RL   Nature 496:498-503(2013).
RN   [7] {ECO:0000313|RefSeq:NP_998231.1}
RP   NUCLEOTIDE SEQUENCE.
RC   STRAIN=AB {ECO:0000313|RefSeq:NP_998231.1};
RX   PubMed=23659367;
RA   Seiliez I., Medale F., Aguirre P., Larquier M., Lanneretonne L.,
RA   Alami-Durante H., Panserat S., Skiba-Cassy S.;
RT   "Postprandial regulation of growth- and metabolism-related factors in
RT   zebrafish.";
RL   Zebrafish 10:237-248(2013).
RN   [8] {ECO:0000313|RefSeq:NP_998231.1}
RP   NUCLEOTIDE SEQUENCE.
RC   STRAIN=AB {ECO:0000313|RefSeq:NP_998231.1};
RX   PubMed=28252024;
RA   Bayes A., Collins M.O., Reig-Viader R., Gou G., Goulding D., Izquierdo A.,
RA   Choudhary J.S., Emes R.D., Grant S.G.;
RT   "Evolution of complexity in the zebrafish synapse proteome.";
RL   Nat. Commun. 8:14613-14613(2017).
RN   [9] {ECO:0000313|RefSeq:NP_998231.1}
RP   NUCLEOTIDE SEQUENCE.
RC   STRAIN=AB {ECO:0000313|RefSeq:NP_998231.1};
RX   PubMed=29203887;
RA   Heid J., Cencioni C., Ripa R., Baumgart M., Atlante S., Milano G.,
RA   Scopece A., Kuenne C., Guenther S., Azzimato V., Farsetti A., Rossi G.,
RA   Braun T., Pompilio G., Martelli F., Zeiher A.M., Cellerino A., Gaetano C.,
RA   Spallotta F.;
RT   "Age-dependent increase of oxidative stress regulates microRNA-29 family
RT   preserving cardiac health.";
RL   Sci. Rep. 7:16839-16839(2017).
RN   [10] {ECO:0000313|RefSeq:NP_998231.1}
RP   NUCLEOTIDE SEQUENCE.
RC   STRAIN=AB {ECO:0000313|RefSeq:NP_998231.1};
RX   PubMed=29913146;
RA   Kuwabara S., Yamaki M., Yu H., Itoh M.;
RT   "Notch signaling regulates the expression of glycolysis-related genes in a
RT   context-dependent manner during embryonic development.";
RL   Biochem. Biophys. Res. Commun. 503:803-808(2018).
RN   [11] {ECO:0000313|RefSeq:NP_998231.1}
RP   NUCLEOTIDE SEQUENCE.
RC   STRAIN=AB {ECO:0000313|RefSeq:NP_998231.1};
RX   PubMed=28946120;
RA   Zhao F., Jiang G., Wei P., Wang H., Ru S.;
RT   "Bisphenol S exposure impairs glucose homeostasis in male zebrafish (Danio
RT   rerio).";
RL   Ecotoxicol. Environ. Saf. 147:794-802(2018).
RN   [12] {ECO:0000313|RefSeq:NP_998231.1}
RP   NUCLEOTIDE SEQUENCE.
RC   STRAIN=AB {ECO:0000313|RefSeq:NP_998231.1};
RX   PubMed=29369637;
RA   Ma D., Tu C., Sheng Q., Yang Y., Kan Z., Guo Y., Shyr Y., Scott I.C.,
RA   Lou X.;
RT   "Dynamics of Zebrafish Heart Regeneration Using an HPLC-ESI-MS/MS
RT   Approach.";
RL   J. Proteome Res. 17:1300-1308(2018).
RN   [13] {ECO:0000313|RefSeq:NP_998231.1}
RP   IDENTIFICATION.
RC   STRAIN=AB {ECO:0000313|RefSeq:NP_998231.1};
RG   RefSeq;
RL   Submitted (NOV-2023) to UniProtKB.
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=ATP + D-glucose = ADP + D-glucose 6-phosphate + H(+);
CC         Xref=Rhea:RHEA:17825, ChEBI:CHEBI:4167, ChEBI:CHEBI:15378,
CC         ChEBI:CHEBI:30616, ChEBI:CHEBI:61548, ChEBI:CHEBI:456216; EC=2.7.1.1;
CC         Evidence={ECO:0000256|ARBA:ARBA00000435};
CC       PhysiologicalDirection=left-to-right; Xref=Rhea:RHEA:17826;
CC         Evidence={ECO:0000256|ARBA:ARBA00000435};
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=ATP + D-hexose = ADP + D-hexose 6-phosphate + H(+);
CC         Xref=Rhea:RHEA:22740, ChEBI:CHEBI:4194, ChEBI:CHEBI:15378,
CC         ChEBI:CHEBI:30616, ChEBI:CHEBI:61567, ChEBI:CHEBI:456216; EC=2.7.1.1;
CC         Evidence={ECO:0000256|ARBA:ARBA00000417};
CC       PhysiologicalDirection=left-to-right; Xref=Rhea:RHEA:22741;
CC         Evidence={ECO:0000256|ARBA:ARBA00000417};
CC   -!- PATHWAY: Carbohydrate degradation; glycolysis; D-glyceraldehyde 3-
CC       phosphate and glycerone phosphate from D-glucose: step 1/4.
CC       {ECO:0000256|ARBA:ARBA00004888}.
CC   -!- PATHWAY: Carbohydrate metabolism; hexose metabolism.
CC       {ECO:0000256|ARBA:ARBA00005028}.
CC   -!- SUBCELLULAR LOCATION: Cytoplasm, cytosol
CC       {ECO:0000256|ARBA:ARBA00004514}. Membrane
CC       {ECO:0000256|ARBA:ARBA00004170}; Peripheral membrane protein
CC       {ECO:0000256|ARBA:ARBA00004170}. Mitochondrion outer membrane
CC       {ECO:0000256|ARBA:ARBA00004450}; Peripheral membrane protein
CC       {ECO:0000256|ARBA:ARBA00004450}.
CC   -!- SIMILARITY: Belongs to the hexokinase family.
CC       {ECO:0000256|ARBA:ARBA00009225}.
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DR   EMBL; BC045496; AAH45496.1; -; mRNA.
DR   RefSeq; NP_998231.1; NM_213066.1.
DR   GeneID; 406339; -.
DR   KEGG; dre:406339; -.
DR   AGR; ZFIN:ZDB-GENE-040426-2017; -.
DR   CTD; 3099; -.
DR   ZFIN; ZDB-GENE-040426-2017; hk2.
DR   OrthoDB; 5481886at2759; -.
DR   PhylomeDB; Q7ZVL7; -.
DR   UniPathway; UPA00109; UER00180.
DR   UniPathway; UPA00242; -.
DR   Proteomes; UP000000437; Alternate scaffold 5.
DR   Proteomes; UP000000437; Chromosome 5.
DR   GO; GO:0005829; C:cytosol; IBA:GO_Central.
DR   GO; GO:0005741; C:mitochondrial outer membrane; IEA:UniProtKB-SubCell.
DR   GO; GO:0005739; C:mitochondrion; IBA:GO_Central.
DR   GO; GO:0005524; F:ATP binding; IEA:UniProtKB-KW.
DR   GO; GO:0008865; F:fructokinase activity; IBA:GO_Central.
DR   GO; GO:0004340; F:glucokinase activity; IBA:GO_Central.
DR   GO; GO:0005536; F:glucose binding; IEA:InterPro.
DR   GO; GO:0046835; P:carbohydrate phosphorylation; IBA:GO_Central.
DR   GO; GO:0051156; P:glucose 6-phosphate metabolic process; IBA:GO_Central.
DR   GO; GO:0006006; P:glucose metabolic process; IBA:GO_Central.
DR   GO; GO:0006096; P:glycolytic process; IBA:GO_Central.
DR   GO; GO:0001678; P:intracellular glucose homeostasis; IBA:GO_Central.
DR   CDD; cd00012; NBD_sugar-kinase_HSP70_actin; 2.
DR   Gene3D; 3.30.420.40; -; 2.
DR   Gene3D; 3.40.367.20; -; 2.
DR   InterPro; IPR043129; ATPase_NBD.
DR   InterPro; IPR001312; Hexokinase.
DR   InterPro; IPR019807; Hexokinase_BS.
DR   InterPro; IPR022673; Hexokinase_C.
DR   InterPro; IPR022672; Hexokinase_N.
DR   PANTHER; PTHR19443; HEXOKINASE; 1.
DR   PANTHER; PTHR19443:SF4; HEXOKINASE-2; 1.
DR   Pfam; PF00349; Hexokinase_1; 2.
DR   Pfam; PF03727; Hexokinase_2; 2.
DR   PRINTS; PR00475; HEXOKINASE.
DR   SUPFAM; SSF53067; Actin-like ATPase domain; 4.
DR   PROSITE; PS00378; HEXOKINASE_1; 2.
DR   PROSITE; PS51748; HEXOKINASE_2; 2.
PE   1: Evidence at protein level;
KW   Acetylation {ECO:0000256|ARBA:ARBA00022990};
KW   Allosteric enzyme {ECO:0000256|ARBA:ARBA00022533};
KW   ATP-binding {ECO:0000256|ARBA:ARBA00022840};
KW   Coiled coil {ECO:0000256|SAM:Coils};
KW   Cytoplasm {ECO:0000256|ARBA:ARBA00022490};
KW   Glycolysis {ECO:0000256|ARBA:ARBA00023152};
KW   Kinase {ECO:0000256|ARBA:ARBA00022777, ECO:0000313|EMBL:AAH45496.1};
KW   Membrane {ECO:0000256|ARBA:ARBA00023136};
KW   Mitochondrion {ECO:0000256|ARBA:ARBA00023128};
KW   Mitochondrion outer membrane {ECO:0000256|ARBA:ARBA00022787};
KW   Nucleotide-binding {ECO:0000256|ARBA:ARBA00022741};
KW   Proteomics identification {ECO:0007829|PeptideAtlas:Q7ZVL7};
KW   Reference proteome {ECO:0000313|Proteomes:UP000000437};
KW   Repeat {ECO:0000256|ARBA:ARBA00022737};
KW   Transferase {ECO:0000256|ARBA:ARBA00022679}.
FT   DOMAIN          22..219
FT                   /note="Hexokinase N-terminal"
FT                   /evidence="ECO:0000259|Pfam:PF00349"
FT   DOMAIN          225..461
FT                   /note="Hexokinase C-terminal"
FT                   /evidence="ECO:0000259|Pfam:PF03727"
FT   DOMAIN          473..669
FT                   /note="Hexokinase N-terminal"
FT                   /evidence="ECO:0000259|Pfam:PF00349"
FT   DOMAIN          676..910
FT                   /note="Hexokinase C-terminal"
FT                   /evidence="ECO:0000259|Pfam:PF03727"
FT   COILED          476..503
FT                   /evidence="ECO:0000256|SAM:Coils"
SQ   SEQUENCE   919 AA;  102163 MW;  BC330D143D6A2891 CRC64;
     MIATQLLAYY FTELHHDHVQ KVDKYLYHMR LSNENLMDVS KRFRKEMDKG LGRDTNPTAA
     VKMLPTFVRS TPDGTETGDF LALDLGGTNF RVLLVKVSSN GMQKVEMENQ IYAISENIMR
     GCGSELFDHI AECLANFLEK LGIKEKKLPL GFTFSFPCQQ TKLDESFLVS WTKGFKASGV
     EGKDVVSLLR KAIRKRGDFD IDIVAVINDT VGTMMTCGYD DHHCEIGLIV GTGTNACYME
     EMRHLELVDG DEGRMCVNME WGAFGDDGAL DDLRTEFDRE IDAGSLNPGK QLFEKMISGM
     YMGELVRLIL VKMAKDGLLF QGHTTPDLLT TGHFQTSFVS AIENRKDDEG IVSAEQVLRG
     LGLDPTPEDC VATQRVCQIV STRAAHLCAA SLAAVLRQIR DNKASDRLRT SIGVDGSVYK
     NHPEFARRLN KMVRSLVPDC DVRFLRSEDG SGKGAAMVTA VAYRLAKQHA ERQRVLDTLR
     LGRDQLLEVK KRMEEEMKRG LAKKTHNNAT VKMLPTFVRS TPDGTERGDF LALDLGGTNF
     RVLLVRVRGG KRRNVEMNNK IYTIPQDITQ GTGEELFDHI VHCIADFLEY MGMKGASLPL
     GFTFSFPCHQ DKLDQGILIK WTKGFKASGC EGEDVASLLK DAIHRCEEFD LDVVAVVNDT
     VGTMMTCGYE DPQCEVGLIV GTGTNACYME EMSNVELVDG DEGRMCVNME WGAFGDQGEL
     DDIWTEFDRA VDDQSTYPGR QRYEKMISGM YLGEIVRNVL LHFTAKGLLF RGKLSERLKT
     RGIFETKFLS QIESDRLALR QVRSILQHLG LTSSTCDDSI LVKEVCSVVS KRAAQLCGAG
     LAAVVDKIRL NRGLNQLSIT VGVDGTLYKL HPHFATIMRE TLRDLAPNCE VSLVQSEDGS
     GKGAALITAV ACRLRDAGK
//