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Protein

NAD-dependent protein deacetylase sirtuin-2

Gene

sirt2

Organism
Danio rerio (Zebrafish) (Brachydanio rerio)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

NAD-dependent protein deacetylase, which deacetylates internal lysines on histone and alpha-tubulin as well as many other proteins such as key transcription factors (By similarity). Participates in the modulation of multiple and diverse biological processes such as cell cycle control, genomic integrity, microtubule dynamics, cell differentiation, metabolic networks, and autophagy. Plays a major role in the control of cell cycle progression and genomic stability. Deacetylates histone H4 at 'Lys-16' (H4K16ac) at the VEGFA promoter. Thereby contributes to regulate expression of vegfa, a key regulator of angiogenesis (PubMed:24940000).By similarity1 Publication

Catalytic activityi

NAD+ + an acetylprotein = nicotinamide + O-acetyl-ADP-ribose + a protein.PROSITE-ProRule annotation

Cofactori

Zn2+By similarityNote: Binds 1 zinc ion per subunit.By similarity

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Active sitei185Proton acceptorPROSITE-ProRule annotation1
Metal bindingi193ZincPROSITE-ProRule annotation1
Metal bindingi198ZincPROSITE-ProRule annotation1
Metal bindingi219ZincPROSITE-ProRule annotation1
Metal bindingi222ZincPROSITE-ProRule annotation1
Binding sitei321NAD; via amide nitrogenBy similarity1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi83 – 87NADBy similarity5
Nucleotide bindingi93 – 95NADBy similarity3
Nucleotide bindingi165 – 168NADBy similarity4
Nucleotide bindingi260 – 261NADBy similarity2
Nucleotide bindingi284 – 286NADBy similarity3

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionHydrolase
LigandMetal-binding, NAD, Zinc

Names & Taxonomyi

Protein namesi
Recommended name:
NAD-dependent protein deacetylase sirtuin-2 (EC:3.5.1.-1 Publication)
Alternative name(s):
Regulatory protein SIR2 homolog 2
SIR2-like protein 2
Gene namesi
Name:sirt2
ORF Names:zgc:77003
OrganismiDanio rerio (Zebrafish) (Brachydanio rerio)
Taxonomic identifieri7955 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiActinopterygiiNeopterygiiTeleosteiOstariophysiCypriniformesCyprinidaeDanio
Proteomesi
  • UP000000437 Componenti: Unplaced

Organism-specific databases

ZFINiZDB-GENE-030131-1028. sirt2.

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Cytoplasm, Nucleus

Pathology & Biotechi

Disruption phenotypei

Morpholino knockdown causes aberrant angiogenesis with excessive intersegmental vessel branching, due to excessive expression of vegfa.1 Publication

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001102621 – 379NAD-dependent protein deacetylase sirtuin-2Add BLAST379

Proteomic databases

PaxDbiQ7ZVK3.

Interactioni

Protein-protein interaction databases

STRINGi7955.ENSDARP00000003412.

Structurei

3D structure databases

ProteinModelPortaliQ7ZVK3.
SMRiQ7ZVK3.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini63 – 337Deacetylase sirtuin-typePROSITE-ProRule annotationAdd BLAST275

Sequence similaritiesi

Belongs to the sirtuin family. Class I subfamily.Curated

Phylogenomic databases

eggNOGiKOG2682. Eukaryota.
COG0846. LUCA.
HOGENOMiHOG000085952.
HOVERGENiHBG057095.
InParanoidiQ7ZVK3.
KOiK11412.
PhylomeDBiQ7ZVK3.

Family and domain databases

Gene3Di3.30.1600.10. 1 hit.
3.40.50.1220. 1 hit.
InterProiView protein in InterPro
IPR029035. DHS-like_NAD/FAD-binding_dom.
IPR003000. Sirtuin.
IPR026591. Sirtuin_cat_small_dom.
IPR017328. Sirtuin_class_I.
IPR026590. Ssirtuin_cat_dom.
PfamiView protein in Pfam
PF02146. SIR2. 1 hit.
PIRSFiPIRSF037938. SIR2_euk. 1 hit.
SUPFAMiSSF52467. SSF52467. 1 hit.
PROSITEiView protein in PROSITE
PS50305. SIRTUIN. 1 hit.

Sequencei

Sequence statusi: Complete.

Q7ZVK3-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSEEVSKRVE EEADTPGLEG QSDDSSDEGD ASGDTEMDFL RSLFSRTLGL
60 70 80 90 100
SPGDKVLDEL TLDSVARYIL SGKCKNIICM VGAGISTSAG IPDFRSPGTG
110 120 130 140 150
LYANLQKYNL PYPEAIFQID YFKKHPEPFF ALARELYPGQ FKPTVYHYFI
160 170 180 190 200
KMLKDKGLLR RCYSQNIDTL ERVAGLEGED LIEAHGTFHT SHCVSFLCRK
210 220 230 240 250
EYSMDWMKNQ IFSEEIPKCD SCGSLVKPDI VFFGESLPSR FFTSMKADFP
260 270 280 290 300
QCDLLIIMGT SLQVQPFASL VSRVSNRCPR LLINMEKTGQ SEFGMGLFSF
310 320 330 340 350
GGGMDFDSDK AYRDVAHLST CDDGCMTLAE LLGWKKELEE MVKREHALID
360 370
SKDAKKTDKE ASQSSKSAVA EAEKTDKTE
Length:379
Mass (Da):42,315
Last modified:June 1, 2003 - v1
Checksum:i1FD8A103DA2EC325
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
BC045510 mRNA. Translation: AAH45510.1.
RefSeqiNP_955890.1. NM_199596.1.
UniGeneiDr.13057.

Genome annotation databases

GeneIDi322309.
KEGGidre:322309.

Similar proteinsi

Entry informationi

Entry nameiSIR2_DANRE
AccessioniPrimary (citable) accession number: Q7ZVK3
Entry historyiIntegrated into UniProtKB/Swiss-Prot: October 31, 2003
Last sequence update: June 1, 2003
Last modified: September 27, 2017
This is version 94 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families