Reviewed, UniProtKB/Swiss-Prot Q7ZVF3 (DHSA_DANRE)

Names and origin

Protein names

Recommended name:
    Succinate dehydrogenase [ubiquinone] flavoprotein subunit, mitochondrial
    EC=1.3.5.1
Alternative name(s):
    Flavoprotein subunit of complex II
      Short name=Fp

Gene names

Name:	sdha
ORF Names:	si:dkeyp-84f11.2

Organism

Danio rerio (Zebrafish) (Brachydanio rerio)

Taxonomic identifier

7955 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Actinopterygii › Neopterygii › Teleostei › Ostariophysi › Cypriniformes › Cyprinidae › Danio

Protein attributes

Sequence length	661 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at transcript level.

General annotation (Comments)

Function	Flavoprotein (FP) subunit of succinate dehydrogenase (SDH) that is involved in complex II of the mitochondrial electron transport chain and is responsible for transferring electrons from succinate to ubiquinone (coenzyme Q) By similarity.
Catalytic activity	Succinate + ubiquinone = fumarate + ubiquinol.
Cofactor	FAD By similarity.
Pathway	Carbohydrate metabolism; tricarboxylic acid cycle.
Subunit structure	Component of complex II composed of four subunits: the flavoprotein (FP) sdha, iron-sulfur protein (IP) sdhb, and a cytochrome b560 composed of sdhc and sdhd By similarity.
Subcellular location	Mitochondrion inner membrane; Peripheral membrane protein; Matrix side By similarity.
Miscellaneous	The complex, present in mitochondria, can be degraded to form EC 1.3.99.1, which no longer reacts with ubiquinone By similarity.
Sequence similarities	Belongs to the FAD-dependent oxidoreductase 2 family. FRD/SDH subfamily.

Ontologies

Keywords
Biological process	Electron transport Transport Tricarboxylic acid cycle
Cellular component	Membrane Mitochondrion Mitochondrion inner membrane
Domain	Transit peptide
Ligand	FAD Flavoprotein
Molecular function	Oxidoreductase
Gene Ontology (GO)
Biological process	electron transport chain Inferred from electronic annotation. Source: UniProtKB-KW transport Inferred from electronic annotation. Source: UniProtKB-KW tricarboxylic acid cycle Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	mitochondrial inner membrane Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	FAD binding Inferred from electronic annotation. Source: InterPro electron carrier activity Inferred from electronic annotation. Source: InterPro succinate dehydrogenase (ubiquinone) activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Transit peptide	1 – 40	40	Mitochondrion By similarity
	Chain	41 – 661	621	Succinate dehydrogenase [ubiquinone] flavoprotein subunit, mitochondrial		PRO_0000344985
Sites
	Active site	337	1	Proton acceptor By similarity
	Binding site	293	1	Substrate By similarity
	Binding site	305	1	Substrate By similarity
	Binding site	404	1	Substrate By similarity
	Binding site	448	1	Substrate By similarity
Amino acid modifications
	Modified residue	96	1	Tele-8alpha-FAD histidine By similarity
Experimental info
	Sequence conflict	18	1	C → S in AAH45885. Ref.2
	Sequence conflict	545	1	D → N in AAH45885. Ref.2

Sequences

	Sequence		Length	Mass (Da)
	Q7ZVF3-1 [UniParc]. Last modified July 22, 2008. Version 2. Checksum: 636BF993BE55D19B	FASTA	661	72,078
	10 20 30 40 50 60 MAAVCAASRV LGTKILSCKS LPAVCQANRQ LHFSIYGKRG DAKISDGVSN QYPVVDHEFD 70 80 90 100 110 120 AVVVGAGGAG LRAAFGLSEA GFNTACVTKL FPTRSHTVAA QGGINAALGN MEQDDWRWHF 130 140 150 160 170 180 YDTVKGSDWL GDQDAIHYMT EQAPAAVVEL ENFGMPFSRT DDGKIYQRAF GGQSLKFGKG 190 200 210 220 230 240 GQAHRCCCVA DRTGHSLLHT LYGRSLRYDT SYFVEYFALD LLMEDGECKG VIALCMEDGS 250 260 270 280 290 300 IHRFRAKNTV IATGGYGRTF FSCTSAHTST GDGNAMVTRA GLPCQDLEFV QFHPTGIYGA 310 320 330 340 350 360 GCLITEGCRG EGGILINSEG ERFMERYAPN AKDLASRDVV SRSMTIEIRE GRGVGPDKDH 370 380 390 400 410 420 VHLQLHHLPP QQLAARLPGI SETAMIFAGV DVTKEPIPVL PTVHYNMGGI PTNYKGQVIT 430 440 450 460 470 480 YKDGQDHVVP GLYACGEAGC ASVHGANRLG ANSLLDLVVF GRACALTIAE IDTPGEKLSP 490 500 510 520 530 540 LKPNAGEASV ANLDKMRYAN GSTRTSEIRL NMQKTMQSHA AVFRTGDVLK EGCVKMESVY 550 560 570 580 590 600 KSMDDIKTFD RGIVWNTDLV ETLELQNLML NAVQTIVSAE ARKESRGAHA REDFKDRVDE 610 620 630 640 650 660 YDYSKPLQGQ VKKPFEQHWR KHTLSYVDPE TGKVTLEYRP VIDSSLDAED CAAIPPAIRS Y

References

[1]	The Danio rerio sequencing project at the Sanger Institute Submitted (FEB-2008) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: Tuebingen.
[2]	NIH - Zebrafish Gene Collection (ZGC) project Submitted (JAN-2003) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Strain: AB.

Cross-references

Sequence databases
EMBLGenBankDDBJ	BX890617 Genomic DNA. Translation: CAK11468.1. BC045885 mRNA. Translation: AAH45885.1.
IPI	IPI00487872.
RefSeq	NP_957204.1.
UniGene	Dr.105233
3D structure databases
HSSP	HSSP built from PDB template 1NEK based on UniProtKB P10444.
SMR	Q7ZVF3. Positions 51-661.
ModBase	Search...
Genome annotation databases
Ensembl	ENSDARG00000016721. Danio rerio. [Contig view]
GeneID	393884.
KEGG	dre:393884.
Organism-specific databases
ZFIN	ZDB-GENE-040426-874. sdha.
Phylogenomic databases
HOVERGEN	Q7ZVF3.
Gene expression databases
ArrayExpress	Q7ZVF3.
Bgee	Q7ZVF3.
Family and domain databases
InterPro	IPR003953. FAD_bind2_N. IPR003952. FRD_SDH_FAD_BS. IPR004112. Fum_Rdtase/Succ_DH_flav_C. IPR011281. Succ_DH_flav_su_fwd. IPR014006. Succ_Dhase_frdA_Gneg. [Graphical view]
Pfam	PF00890. FAD_binding_2. 1 hit. PF02910. Succ_DH_flav_C. 1 hit. [Graphical view]
TIGRFAMs	TIGR01816. sdhA_forward. 1 hit. TIGR01812. sdhA_frdA_Gneg. 1 hit.
PROSITE	PS00504. FRD_SDH_FAD_BINDING. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

DHSA_DANRE

Accession

Primary (citable) accession number: Q7ZVF3
Secondary accession number(s): Q1LV99

Entry history

Integrated into UniProtKB/Swiss-Prot:	July 22, 2008
Last sequence update:	July 22, 2008
Last modified:	June 16, 2009
This is version 58 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

Zebrafish annotation project