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Q7ZVA6 (IF4A3_DANRE) Reviewed, UniProtKB/Swiss-Prot

Last modified April 16, 2014. Version 79. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Eukaryotic initiation factor 4A-III

Short name=eIF-4A-III
Short name=eIF4A-III
EC=3.6.4.13
Alternative name(s):
ATP-dependent RNA helicase DDX48
ATP-dependent RNA helicase eIF4A-3
DEAD box protein 48
Eukaryotic translation initiation factor 4A isoform 3
Gene names
Name:eif4a3
Synonyms:ddx48
OrganismDanio rerio (Zebrafish) (Brachydanio rerio) [Reference proteome]
Taxonomic identifier7955 [NCBI]
Taxonomic lineageEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiActinopterygiiNeopterygiiTeleosteiOstariophysiCypriniformesCyprinidaeDanio

Protein attributes

Sequence length406 AA.
Sequence statusComplete.
Protein existenceEvidence at transcript level

General annotation (Comments)

Function

ATP-dependent RNA helicase. Core component of the splicing-dependent multiprotein exon junction complex (EJC) deposited at splice junctions on mRNAs. The EJC is a dynamic structure consisting of core proteins and several peripheral nuclear and cytoplasmic associated factors that join the complex only transiently either during EJC assembly or during subsequent mRNA metabolism. The EJC marks the position of the exon-exon junction in the mature mRNA for the gene expression machinery and the core components remain bound to spliced mRNAs throughout all stages of mRNA metabolism thereby influencing downstream processes including nuclear mRNA export, subcellular mRNA localization, translation efficiency and nonsense-mediated mRNA decay (NMD). Binds spliced mRNA in sequence-independent manner, 20-24 nucleotides upstream of mRNA exon-exon junctions By similarity.

Catalytic activity

ATP + H2O = ADP + phosphate.

Subunit structure

Part of the EJC core complex that contains casc3, eif4a3, magoh and rbm8a By similarity.

Subcellular location

Nucleus By similarity. Nucleus speckle By similarity. Cytoplasm By similarity. Note: Nucleocytoplasmic shuttling protein. Travels to the cytoplasm as part of the exon junction complex (EJC) bound to mRNA By similarity.

Sequence similarities

Belongs to the DEAD box helicase family. eIF4A subfamily.

Contains 1 helicase ATP-binding domain.

Contains 1 helicase C-terminal domain.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 406406Eukaryotic initiation factor 4A-III
PRO_0000379480

Regions

Domain64 – 234171Helicase ATP-binding
Domain245 – 406162Helicase C-terminal
Nucleotide binding77 – 848ATP By similarity
Motif33 – 6129Q motif
Motif182 – 1854DEAD box

Sequences

Sequence LengthMass (Da)Tools
Q7ZVA6 [UniParc].

Last modified June 1, 2003. Version 1.
Checksum: 765707C2F2E60695

FASTA40646,468
        10         20         30         40         50         60 
MATAVVPVRK RILKEEDMTK IEFETSEEVD VTPTFDTMGL REDLLRGIYA YGFEKPSAIQ 

        70         80         90        100        110        120 
QRAIKQIIKG RDVIAQSQSG TGKTATFCVS VLQCLDIQVR ETQALILAPT RELAGQIQKV 

       130        140        150        160        170        180 
LLALGDYMNV QCHACIGGTN VGEDIRKLDY GQHVVAGTPG RVFDMIRRRS LRTRAIKMLV 

       190        200        210        220        230        240 
LDEADEMLNK GFKEQIYDVY RYLPPATQVC LISATLPHEI LEMTNKFMTD PIRILVKRDE 

       250        260        270        280        290        300 
LTLEGIKQFF VAVEREEWKF DTLCDLYDTL TITQAVIFCN TKRKVDWLTE KMREANFTVS 

       310        320        330        340        350        360 
SMHGDMPQKE RESIMKEFRS GASRVLISTD VWARGLDVSQ VSLIINYDLP NNRELYIHRI 

       370        380        390        400 
GRSGRYGRKG VAINFVKNDD IRILRDIEQY YSTQIDEMPM NVADLI 

« Hide

References

[1]NIH - Zebrafish Gene Collection (ZGC) project
Submitted (JAN-2003) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
BC045939 mRNA. Translation: AAH45939.1.
RefSeqNP_957372.1. NM_201078.1.
UniGeneDr.78203.

3D structure databases

ProteinModelPortalQ7ZVA6.
SMRQ7ZVA6. Positions 33-406.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

IntActQ7ZVA6. 1 interaction.
MINTMINT-8283544.
STRING7955.ENSDARP00000027276.

Proteomic databases

PRIDEQ7ZVA6.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

GeneID394053.
KEGGdre:394053.

Organism-specific databases

CTD9775.
ZFINZDB-GENE-040426-915. eif4a3.

Phylogenomic databases

eggNOGCOG0513.
HOGENOMHOG000268797.
InParanoidQ7ZVA6.
KOK13025.
PhylomeDBQ7ZVA6.

Family and domain databases

Gene3D3.40.50.300. 2 hits.
InterProIPR011545. DNA/RNA_helicase_DEAD/DEAH_N.
IPR014001. Helicase_ATP-bd.
IPR001650. Helicase_C.
IPR027417. P-loop_NTPase.
IPR000629. RNA-helicase_DEAD-box_CS.
IPR014014. RNA_helicase_DEAD_Q_motif.
[Graphical view]
PfamPF00270. DEAD. 1 hit.
PF00271. Helicase_C. 1 hit.
[Graphical view]
SMARTSM00487. DEXDc. 1 hit.
SM00490. HELICc. 1 hit.
[Graphical view]
SUPFAMSSF52540. SSF52540. 1 hit.
PROSITEPS00039. DEAD_ATP_HELICASE. 1 hit.
PS51192. HELICASE_ATP_BIND_1. 1 hit.
PS51194. HELICASE_CTER. 1 hit.
PS51195. Q_MOTIF. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

NextBio20815014.
PROQ7ZVA6.

Entry information

Entry nameIF4A3_DANRE
AccessionPrimary (citable) accession number: Q7ZVA6
Entry history
Integrated into UniProtKB/Swiss-Prot: July 7, 2009
Last sequence update: June 1, 2003
Last modified: April 16, 2014
This is version 79 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

Translation initiation factors

List of translation initiation factor entries