Eukaryotic initiation factor 4A-III - Danio rerio (Zebrafish)

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Q7ZVA6 (IF4A3_DANRE) Reviewed, UniProtKB/Swiss-Prot

Last modified May 29, 2013. Version 73. History...

Clusters with 100%, 90%, 50% identity |

Documents (2) |

Third-party data

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Names·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·Documents Customize order

Names and origin

Protein names

Recommended name:
Eukaryotic initiation factor 4A-III
Short name=eIF-4A-III
Short name=eIF4A-III
EC=3.6.4.13

Alternative name(s):
ATP-dependent RNA helicase DDX48
ATP-dependent RNA helicase eIF4A-3
DEAD box protein 48
Eukaryotic translation initiation factor 4A isoform 3

Gene names

Name:	eif4a3
Synonyms:	ddx48

Organism

Danio rerio (Zebrafish) (Brachydanio rerio) [Reference proteome]

Taxonomic identifier

7955 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Actinopterygii › Neopterygii › Teleostei › Ostariophysi › Cypriniformes › Cyprinidae › Danio

Protein attributes

Sequence length	406 AA.
Sequence status	Complete.
Protein existence	Evidence at transcript level

General annotation (Comments)

Function	ATP-dependent RNA helicase. Component of a splicing-dependent multiprotein exon junction complex (EJC) deposited at splice junction on mRNAs. The EJC is a dynamic structure consisting of a few core proteins and several more peripheral nuclear and cytoplasmic associated factors that join the complex only transiently either during EJC assembly or during subsequent mRNA metabolism. Core components of the EJC, that remains bound to spliced mRNAs throughout all stages of mRNA metabolism, functions to mark the position of the exon-exon junction in the mature mRNA and thereby influences downstream processes of gene expression including mRNA splicing, nuclear mRNA export, subcellular mRNA localization, translation efficiency and nonsense-mediated mRNA decay (NMD). Constitutes at least part of the platform anchoring other EJC proteins to spliced mRNAs. Binds spliced mRNA in sequence-independent manner, 20-24 nucleotides upstream of mRNA exon-exon junctions By similarity.
Catalytic activity	ATP + H₂O = ADP + phosphate.
Subunit structure	Part of the EJC core complex that contains casc3, eif4a3, magoh and rbm8a By similarity.
Subcellular location	Nucleus By similarity. Nucleus speckle By similarity. Cytoplasm By similarity. Note: Nucleocytoplasmic shuttling protein. Travels to the cytoplasm as part of the exon junction complex (EJC) bound to mRNA By similarity.
Sequence similarities	Belongs to the DEAD box helicase family. eIF4A subfamily. Contains 1 helicase ATP-binding domain. Contains 1 helicase C-terminal domain.

Ontologies

Keywords
Biological process	Nonsense-mediated mRNA decay Translation regulation Transport mRNA processing mRNA splicing mRNA transport
Cellular component	Cytoplasm Nucleus
Ligand	ATP-binding Nucleotide-binding RNA-binding
Molecular function	Helicase Hydrolase
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	RNA splicing Inferred from electronic annotation. Source: UniProtKB-KW mRNA processing Inferred from electronic annotation. Source: UniProtKB-KW mRNA transport Inferred from electronic annotation. Source: UniProtKB-KW nuclear-transcribed mRNA catabolic process, nonsense-mediated decay Inferred from electronic annotation. Source: UniProtKB-KW regulation of translation Inferred from electronic annotation. Source: UniProtKB-KW
Cellular_component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell nuclear speck Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular_function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW ATP-dependent helicase activity Inferred from electronic annotation. Source: InterPro RNA binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Chain

1 – 406

406

Eukaryotic initiation factor 4A-III

PRO_0000379480

Regions

Domain

64 – 234

171

Helicase ATP-binding

Domain

245 – 406

162

Helicase C-terminal

Nucleotide binding

77 – 84

ATP By similarity

Motif

33 – 61

Q motif

Motif

182 – 185

DEAD box

Sequences

Sequence

Length

Mass (Da)

Tools

Q7ZVA6 [UniParc].

Last modified June 1, 2003. Version 1.
Checksum: 765707C2F2E60695
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FASTA

406

46,468

        10         20         30         40         50         60 
MATAVVPVRK RILKEEDMTK IEFETSEEVD VTPTFDTMGL REDLLRGIYA YGFEKPSAIQ 

        70         80         90        100        110        120 
QRAIKQIIKG RDVIAQSQSG TGKTATFCVS VLQCLDIQVR ETQALILAPT RELAGQIQKV 

       130        140        150        160        170        180 
LLALGDYMNV QCHACIGGTN VGEDIRKLDY GQHVVAGTPG RVFDMIRRRS LRTRAIKMLV 

       190        200        210        220        230        240 
LDEADEMLNK GFKEQIYDVY RYLPPATQVC LISATLPHEI LEMTNKFMTD PIRILVKRDE 

       250        260        270        280        290        300 
LTLEGIKQFF VAVEREEWKF DTLCDLYDTL TITQAVIFCN TKRKVDWLTE KMREANFTVS 

       310        320        330        340        350        360 
SMHGDMPQKE RESIMKEFRS GASRVLISTD VWARGLDVSQ VSLIINYDLP NNRELYIHRI 

       370        380        390        400 
GRSGRYGRKG VAINFVKNDD IRILRDIEQY YSTQIDEMPM NVADLI

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References

[1]	NIH - Zebrafish Gene Collection (ZGC) project Submitted (JAN-2003) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].

Cross-references

Sequence databases
EMBL GenBank DDBJ	BC045939 mRNA. Translation: AAH45939.1.
IPI	IPI00481285.
RefSeq	NP_957372.1. NM_201078.1.
UniGene	Dr.78203.
3D structure databases
ProteinModelPortal	Q7ZVA6.
SMR	Q7ZVA6. Positions 33-406.
ModBase	Search...
Protein-protein interaction databases
MINT	MINT-8283544.
STRING	7955.ENSDARP00000027276.
Proteomic databases
PRIDE	Q7ZVA6.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	394053.
KEGG	dre:394053.
Organism-specific databases
CTD	9775.
ZFIN	ZDB-GENE-040426-915. eif4a3.
Phylogenomic databases
eggNOG	COG0513.
HOGENOM	HOG000268797.
InParanoid	Q7ZVA6.
KO	K13025.
OrthoDB	EOG4229JV.
Family and domain databases
InterPro	IPR011545. DNA/RNA_helicase_DEAD/DEAH_N. IPR014001. Helicase_ATP-bd. IPR001650. Helicase_C. IPR027417. P-loop_NTPase. IPR000629. RNA-helicase_DEAD-box_CS. IPR014014. RNA_helicase_DEAD_Q_motif. [Graphical view]
Pfam	PF00270. DEAD. 1 hit. PF00271. Helicase_C. 1 hit. [Graphical view]
SMART	SM00487. DEXDc. 1 hit. SM00490. HELICc. 1 hit. [Graphical view]
SUPFAM	SSF52540. SSF52540. 2 hits.
PROSITE	PS00039. DEAD_ATP_HELICASE. 1 hit. PS51192. HELICASE_ATP_BIND_1. 1 hit. PS51194. HELICASE_CTER. 1 hit. PS51195. Q_MOTIF. 1 hit. [Graphical view]
ProtoNet	Search...
Other
NextBio	20815014.

Entry information

Entry name

IF4A3_DANRE

Accession

Primary (citable) accession number: Q7ZVA6

Entry history

Integrated into UniProtKB/Swiss-Prot:	July 7, 2009
Last sequence update:	June 1, 2003
Last modified:	May 29, 2013
This is version 73 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program

Relevant documents

Translation initiation factors

List of translation initiation factor entries

SIMILARITY comments

Index of protein domains and families

Names·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·Documents

Preferred order of sections

Names and origin

Protein attributes

General annotation (Comments)

Ontologies

Sequence annotation (Features)

Molecule processing

Regions

Sequences

References

Cross-references

Sequence databases

3D structure databases

Protein-protein interaction databases

Proteomic databases

Protocols and materials databases

Genome annotation databases

Organism-specific databases

Phylogenomic databases

Family and domain databases

Other

Entry information

Relevant documents