Reviewed, UniProtKB/Swiss-Prot Q7ZV14 (GPX8_DANRE)

Names and origin

Protein names

Recommended name:
    Probable glutathione peroxidase 8
    EC=1.11.1.9

Gene names

Name:	gpx8
ORF Names:	zgc:56280

Organism

Danio rerio (Zebrafish) (Brachydanio rerio)

Taxonomic identifier

7955 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Actinopterygii › Neopterygii › Teleostei › Ostariophysi › Cypriniformes › Cyprinidae › Danio

Protein attributes

Sequence length	210 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at transcript level.

General annotation (Comments)

Catalytic activity	2 glutathione + H(2)O(2) = glutathione disulfide + 2 H(2)O.
Subcellular location	Membrane; Single-pass membrane proteinPotential.
Sequence similarities	Belongs to the glutathione peroxidase family.
Caution	Ser-80 is present instead of the conserved Cys/Sec which is expected to be the active site residue.

Ontologies

Keywords
Cellular component	Membrane
Domain	Transmembrane
Molecular function	Oxidoreductase Peroxidase
Gene Ontology (GO)
Biological process	oxidation reduction Inferred from electronic annotation. Source: UniProtKB-KW response to oxidative stress Inferred from electronic annotation. Source: InterPro
Cellular component	integral to membrane Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	glutathione peroxidase activity Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 210	210	Probable glutathione peroxidase 8		PRO_0000317758
Regions
	Transmembrane	13 – 35	23	Potential

Sequences

	Sequence		Length	Mass (Da)
	Q7ZV14-1 [UniParc]. Last modified June 1, 2003. Version 1. Checksum: 3973B501C27B0DA8	FASTA	210	23,806
	10 20 30 40 50 60 MEALGGYPSK SSASRAGLFK VLLSVALCMG SLYLLQNKLS KSRKTKDFYS YEVKDARGRT 70 80 90 100 110 120 VSLEKYRGKV SLVVNVASGS ELTEQSYRAL QELHRELGTS HFNVLAFPCS QYGDTESGTS 130 140 150 160 170 180 REIEAFAKSN YGVTFPIFNK IKIMGSEAEP AFRFLTDSVQ KIPRWNFWKF LVSPEGQVVR 190 200 210 FWKPEEPVSD IRKEATTLVR NIILKKRQEL

References

[1]	NIH - Zebrafish Gene Collection (ZGC) project Submitted (JAN-2003) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].

Cross-references

Sequence databases
EMBLGenBankDDBJ	BC046044 mRNA. Translation: AAH46044.1.
RefSeq	NP_956516.1.
UniGene	Dr.107413
3D structure databases
HSSP	HSSP built from PDB template 1GP1 based on UniProtKB P00435.
ModBase	Search...
Genome annotation databases
Ensembl	ENSDARG00000013302. Danio rerio. [Contig view]
GeneID	393191.
KEGG	dre:393191.
Organism-specific databases
ZFIN	ZDB-GENE-040426-965. zgc:56280.
Phylogenomic databases
HOGENOM	Q7ZV14.
HOVERGEN	Q7ZV14.
Gene expression databases
ArrayExpress	Q7ZV14.
Family and domain databases
InterPro	IPR000886. ER_targeting_sequence. IPR013376. Glut_perox_Gpx7. IPR000889. Glut_peroxidase. IPR012335. Thioredoxin_fold. [Graphical view]
Gene3D	G3DSA:3.40.30.10. Thioredoxin_fold. 1 hit.
PANTHER	PTHR11592. Glut_peroxidase. 1 hit.
Pfam	PF00255. GSHPx. 1 hit. [Graphical view]
PIRSF	PIRSF000303. Glutathion_perox. 1 hit.
PRINTS	PR01011. GLUTPROXDASE.
TIGRFAMs	TIGR02540. gpx7. 1 hit.
PROSITE	PS00014. ER_TARGET. 1 hit. Uncertain. PS00460. GLUTATHIONE_PEROXID_1. False negative. PS00763. GLUTATHIONE_PEROXID_2. False negative. PS51355. GLUTATHIONE_PEROXID_3. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

GPX8_DANRE

Accession

Primary (citable) accession number: Q7ZV14

Entry history

Integrated into UniProtKB/Swiss-Prot:	February 5, 2008
Last sequence update:	June 1, 2003
Last modified:	November 25, 2008
This is version 45 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

Zebrafish annotation project