Q7ZV14 (GPX8_DANRE) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Probable glutathione peroxidase 8
Short name=GPx-8
Short name=GSHPx-8
EC=1.11.1.9

Gene names

Name:	gpx8
ORF Names:	zgc:56280

Organism

Danio rerio (Zebrafish) (Brachydanio rerio) [Reference proteome]

Taxonomic identifier

7955 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Deuterostomia › Chordata › Craniata › Vertebrata › Gnathostomata › Teleostomi › Euteleostomi › Actinopterygii › Actinopteri › Neopterygii › Teleostei › Elopocephala › Clupeocephala › Otocephala › Ostariophysi › Otophysi › Cypriniphysi › Cypriniformes › Cyprinoidea › Cyprinidae › Danio

Protein attributes

Sequence length	210 AA.
Sequence status	Complete.
Protein existence	Evidence at transcript level

General annotation (Comments)

Catalytic activity	2 glutathione + H2O2 = glutathione disulfide + 2 H2O.
Subcellular location	Membrane; Single-pass membrane protein Potential.
Sequence similarities	Belongs to the glutathione peroxidase family.
Caution	Ser-80 is present instead of the conserved Cys/Sec which is expected to be the active site residue.

Ontologies

Keywords
Cellular component	Membrane
Domain	Transmembrane Transmembrane helix
Molecular function	Oxidoreductase Peroxidase
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	response to oxidative stress Inferred from electronic annotation. Source: InterPro
Cellular_component	integral to membrane Inferred from electronic annotation. Source: UniProtKB-KW
Molecular_function	glutathione peroxidase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 210	210	Probable glutathione peroxidase 8		PRO_0000317758
Regions
	Transmembrane	13 – 35	23	Helical; Potential

Sequences

	Sequence		Length	Mass (Da)
	Q7ZV14 [UniParc]. Last modified June 1, 2003. Version 1. Checksum: 3973B501C27B0DA8	FASTA	210	23,806
	10 20 30 40 50 60 MEALGGYPSK SSASRAGLFK VLLSVALCMG SLYLLQNKLS KSRKTKDFYS YEVKDARGRT 70 80 90 100 110 120 VSLEKYRGKV SLVVNVASGS ELTEQSYRAL QELHRELGTS HFNVLAFPCS QYGDTESGTS 130 140 150 160 170 180 REIEAFAKSN YGVTFPIFNK IKIMGSEAEP AFRFLTDSVQ KIPRWNFWKF LVSPEGQVVR 190 200 210 FWKPEEPVSD IRKEATTLVR NIILKKRQEL

References

[1]	NIH - Zebrafish Gene Collection (ZGC) project Submitted (JAN-2003) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].

Cross-references

Sequence databases
EMBL GenBank DDBJ	BC046044 mRNA. Translation: AAH46044.1.
IPI	IPI00504014.
RefSeq	NP_956516.1. NM_200222.1.
UniGene	Dr.107413.
3D structure databases
HSSP	HSSP built from PDB template 2F8A based on UniProtKB P07203.
ProteinModelPortal	Q7ZV14.
SMR	Q7ZV14. Positions 48-210.
ModBase	Search...
Protein-protein interaction databases
STRING	7955.ENSDARP00000011955.
Proteomic databases
PRIDE	Q7ZV14.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	393191.
KEGG	dre:393191.
Organism-specific databases
CTD	493869.
ZFIN	ZDB-GENE-040426-965. gpx8.
Phylogenomic databases
eggNOG	COG0386.
HOGENOM	HOG000277054.
HOVERGEN	HBG004333.
InParanoid	Q7ZV14.
KO	K00432.
OrthoDB	EOG4TXBSZ.
Family and domain databases
Gene3D	3.40.30.10. 1 hit.
InterPro	IPR013376. Glut_perox_Gpx7. IPR000889. Glutathione_peroxidase. IPR012336. Thioredoxin-like_fold. [Graphical view]
PANTHER	PTHR11592. PTHR11592. 1 hit.
Pfam	PF00255. GSHPx. 1 hit. [Graphical view]
PIRSF	PIRSF000303. Glutathion_perox. 1 hit.
PRINTS	PR01011. GLUTPROXDASE.
SUPFAM	SSF52833. Thiordxn-like_fd. 1 hit.
TIGRFAMs	TIGR02540. gpx7. 1 hit.
PROSITE	PS00014. ER_TARGET. 1 hit. Uncertain. PS00460. GLUTATHIONE_PEROXID_1. False negative. PS00763. GLUTATHIONE_PEROXID_2. False negative. PS51355. GLUTATHIONE_PEROXID_3. 1 hit. [Graphical view]
ProtoNet	Search...
Other
NextBio	20814256.

Entry information

Entry name

GPX8_DANRE

Accession

Primary (citable) accession number: Q7ZV14

Entry history

Integrated into UniProtKB/Swiss-Prot:	February 5, 2008
Last sequence update:	June 1, 2003
Last modified:	May 1, 2013
This is version 79 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program