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Protein

Protein Mdm4

Gene

mdm4

Organism
Danio rerio (Zebrafish) (Brachydanio rerio)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

Inhibits p53- and p73-mediated cell cycle arrest and apoptosis by binding its transcriptional activation domain.By similarity

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Zinc fingeri305 – 33430RanBP2-typePROSITE-ProRule annotationAdd
BLAST
Zinc fingeri443 – 48442RING-type; degenerateAdd
BLAST

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Ligandi

Metal-binding, Zinc

Names & Taxonomyi

Protein namesi
Recommended name:
Protein Mdm4
Alternative name(s):
Double minute 4 protein
Mdm2-like p53-binding protein
Protein Mdmx
p53-binding protein Mdm4
Gene namesi
Name:mdm4
Synonyms:mdmx
OrganismiDanio rerio (Zebrafish) (Brachydanio rerio)
Taxonomic identifieri7955 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiActinopterygiiNeopterygiiTeleosteiOstariophysiCypriniformesCyprinidaeDanio
Proteomesi
  • UP000000437 Componenti: Unplaced

Organism-specific databases

ZFINiZDB-GENE-030131-6872. mdm4.

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 496496Protein Mdm4PRO_0000331517Add
BLAST

Proteomic databases

PaxDbiQ7ZUW7.

Interactioni

Subunit structurei

Binds to p73 (By similarity). Interacts directly with p53.By similarity1 Publication

Protein-protein interaction databases

BioGridi85461. 1 interaction.
STRINGi7955.ENSDARP00000074651.

Structurei

Secondary structure

1
496
Legend: HelixTurnBeta strand
Show more details
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Beta strandi23 – 264Combined sources
Helixi28 – 369Combined sources
Beta strandi42 – 454Combined sources
Helixi46 – 5914Combined sources
Beta strandi65 – 673Combined sources
Beta strandi70 – 723Combined sources
Helixi77 – 826Combined sources
Beta strandi84 – 885Combined sources
Helixi93 – 10210Combined sources
Beta strandi103 – 1053Combined sources

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
2Z5SX-ray2.30M/N/O15-140[»]
2Z5TX-ray2.30M/N/O15-140[»]
3DACX-ray1.80A/M15-129[»]
4N5TX-ray1.70A17-106[»]
ProteinModelPortaliQ7ZUW7.
SMRiQ7ZUW7. Positions 21-106, 306-345, 437-496.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiQ7ZUW7.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini23 – 9876SWIBAdd
BLAST

Motif

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Motifi448 – 4514Nuclear localization signalSequence analysis

Compositional bias

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Compositional biasi362 – 3676Poly-Glu

Sequence similaritiesi

Belongs to the MDM2/MDM4 family.Curated
Contains 1 RanBP2-type zinc finger.PROSITE-ProRule annotation
Contains 1 RING-type zinc finger.Curated
Contains 1 SWIB domain.Curated

Zinc finger

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Zinc fingeri305 – 33430RanBP2-typePROSITE-ProRule annotationAdd
BLAST
Zinc fingeri443 – 48442RING-type; degenerateAdd
BLAST

Keywords - Domaini

Zinc-finger

Phylogenomic databases

eggNOGiENOG410IE9V. Eukaryota.
ENOG410XSWN. LUCA.
HOGENOMiHOG000293341.
HOVERGENiHBG013472.
InParanoidiQ7ZUW7.
KOiK10127.
PhylomeDBiQ7ZUW7.

Family and domain databases

Gene3Di1.10.245.10. 1 hit.
InterProiIPR015458. MDM4.
IPR016495. p53_neg-reg_MDM_2/4.
IPR003121. SWIB_MDM2_domain.
IPR001876. Znf_RanBP2.
[Graphical view]
PfamiPF02201. SWIB. 1 hit.
[Graphical view]
PIRSFiPIRSF500699. MDM4. 1 hit.
PIRSF006748. p53_MDM_2/4. 1 hit.
SUPFAMiSSF47592. SSF47592. 1 hit.
SSF90209. SSF90209. 1 hit.
PROSITEiPS01358. ZF_RANBP2_1. 1 hit.
PS50199. ZF_RANBP2_2. 1 hit.
[Graphical view]

Sequences (2)i

Sequence statusi: Complete.

This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 1 (identifier: Q7ZUW7-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MTSLASSSQL PGSCRTLPGE GTQVHPRAPL LQILKVAGAQ EEVFTLKEVM
60 70 80 90 100
HYLGQYIMMK QLYDKQRQHI VHCHDDPLGE LLEVGSFSVK NPSPVYEMLK
110 120 130 140 150
RNLVILNNSD AAKNLSVGKD SNESPSEDPG QVSSGSINSA QPLIAGSSST
160 170 180 190 200
GTTQSCSQRR PRDPDEDSSD GLPRSACKRP KLDVTLEEWD LSGLPWWFLG
210 220 230 240 250
NLRSNYTRRS NGSTDIHTNQ LSPGQDEDTA IVSDTTDDLW FLNEAESEQV
260 270 280 290 300
SVEIKEAVLE QGSDGESPHE DEDTGKDSKD DGKMQEEQEE DSQCLSDDTD
310 320 330 340 350
TEISTQDAWQ CSECRKFNTP LQRYCMRCWA LRKDWYKDCP RLVHSISVPD
360 370 380 390 400
IPACSSRPER DEDEEEEDDD GIDMPDCLRT VSDPVVLPSH RVSRNIPSSS
410 420 430 440 450
ASSSKGKGPS QIHHHFQETS EGDSQDTLDM ETEYQPEALL EPCKLCRVRP
460 470 480 490
RNGNIIHGRT AHLITCFPCA RKLHKFHAPC PGCGQVIQKV IKTFIA
Length:496
Mass (Da):55,241
Last modified:April 29, 2008 - v2
Checksum:iBACE83EDE11112B5
GO
Isoform 2 (identifier: Q7ZUW7-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     220-224: Missing.

Note: No experimental confirmation available.
Show »
Length:491
Mass (Da):54,759
Checksum:iB9A92CF922B154AA
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti254 – 2541I → M in AAH47801 (Ref. 1) Curated
Sequence conflicti270 – 2701E → G in AAH47801 (Ref. 1) Curated
Sequence conflicti342 – 3421L → P in AAH47801 (Ref. 1) Curated
Sequence conflicti416 – 4161F → L in AAH47801 (Ref. 1) Curated

Alternative sequence

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Alternative sequencei220 – 2245Missing in isoform 2. 1 PublicationVSP_033237

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
BC047801 mRNA. Translation: AAH47801.1.
BC097069 mRNA. Translation: AAH97069.1.
RefSeqiNP_997897.1. NM_212732.1.
UniGeneiDr.48996.

Genome annotation databases

GeneIDi334932.
KEGGidre:334932.

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
BC047801 mRNA. Translation: AAH47801.1.
BC097069 mRNA. Translation: AAH97069.1.
RefSeqiNP_997897.1. NM_212732.1.
UniGeneiDr.48996.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
2Z5SX-ray2.30M/N/O15-140[»]
2Z5TX-ray2.30M/N/O15-140[»]
3DACX-ray1.80A/M15-129[»]
4N5TX-ray1.70A17-106[»]
ProteinModelPortaliQ7ZUW7.
SMRiQ7ZUW7. Positions 21-106, 306-345, 437-496.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi85461. 1 interaction.
STRINGi7955.ENSDARP00000074651.

Proteomic databases

PaxDbiQ7ZUW7.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

GeneIDi334932.
KEGGidre:334932.

Organism-specific databases

CTDi4194.
ZFINiZDB-GENE-030131-6872. mdm4.

Phylogenomic databases

eggNOGiENOG410IE9V. Eukaryota.
ENOG410XSWN. LUCA.
HOGENOMiHOG000293341.
HOVERGENiHBG013472.
InParanoidiQ7ZUW7.
KOiK10127.
PhylomeDBiQ7ZUW7.

Miscellaneous databases

EvolutionaryTraceiQ7ZUW7.
PROiQ7ZUW7.

Family and domain databases

Gene3Di1.10.245.10. 1 hit.
InterProiIPR015458. MDM4.
IPR016495. p53_neg-reg_MDM_2/4.
IPR003121. SWIB_MDM2_domain.
IPR001876. Znf_RanBP2.
[Graphical view]
PfamiPF02201. SWIB. 1 hit.
[Graphical view]
PIRSFiPIRSF500699. MDM4. 1 hit.
PIRSF006748. p53_MDM_2/4. 1 hit.
SUPFAMiSSF47592. SSF47592. 1 hit.
SSF90209. SSF90209. 1 hit.
PROSITEiPS01358. ZF_RANBP2_1. 1 hit.
PS50199. ZF_RANBP2_2. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. NIH - Zebrafish Gene Collection (ZGC) project
    Submitted (JUN-2005) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORMS 1 AND 2).
    Strain: AB.
  2. Cited for: X-RAY CRYSTALLOGRAPHY (2.3 ANGSTROMS) OF 15-140 IN COMPLEX WITH P53.

Entry informationi

Entry nameiMDM4_DANRE
AccessioniPrimary (citable) accession number: Q7ZUW7
Secondary accession number(s): Q4V944
Entry historyi
Integrated into UniProtKB/Swiss-Prot: April 29, 2008
Last sequence update: April 29, 2008
Last modified: June 8, 2016
This is version 83 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.