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Q7ZUM8 (UBP44_DANRE) Reviewed, UniProtKB/Swiss-Prot

Last modified April 16, 2014. Version 65. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Ubiquitin carboxyl-terminal hydrolase 44

EC=3.4.19.12
Alternative name(s):
Deubiquitinating enzyme 44
Ubiquitin thioesterase 44
Ubiquitin-specific-processing protease 44
Gene names
Name:usp44
ORF Names:zgc:55661
OrganismDanio rerio (Zebrafish) (Brachydanio rerio) [Reference proteome]
Taxonomic identifier7955 [NCBI]
Taxonomic lineageEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiActinopterygiiNeopterygiiTeleosteiOstariophysiCypriniformesCyprinidaeDanio

Protein attributes

Sequence length695 AA.
Sequence statusComplete.
Protein existenceEvidence at transcript level

General annotation (Comments)

Function

Deubiquitinase that plays a key role in the spindle assembly checkpoint by preventing premature anaphase onset. Acts by specifically mediating deubiquitination of cdc20, a negative regulator of the anaphase promoting complex/cyclosome (APC/C) By similarity.

Catalytic activity

Thiol-dependent hydrolysis of ester, thioester, amide, peptide and isopeptide bonds formed by the C-terminal Gly of ubiquitin (a 76-residue protein attached to proteins as an intracellular targeting signal).

Subcellular location

Nucleus By similarity.

Sequence similarities

Belongs to the peptidase C19 family. USP44 subfamily.

Contains 1 UBP-type zinc finger.

Contains 1 USP domain.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 695695Ubiquitin carboxyl-terminal hydrolase 44
PRO_0000395812

Regions

Domain295 – 684390USP
Zinc finger24 – 8562UBP-type

Sites

Active site3041Nucleophile By similarity
Active site6421Proton acceptor By similarity

Sequences

Sequence LengthMass (Da)Tools
Q7ZUM8 [UniParc].

Last modified June 1, 2003. Version 1.
Checksum: CEAE12134C67ED9F

FASTA69579,926
        10         20         30         40         50         60 
MDRCKHVGRL RLAQDHSILN PQKWHCVDCN TTESVWACLS CSHVACGRYI EEHALQHFKE 

        70         80         90        100        110        120 
QHHPLALEVN ELYVYCYLCD DYVLNDNATG DLKLLRSTLS AIKSQCYEVT TRSGRTLRSS 

       130        140        150        160        170        180 
SANGDQLSPS TQELQLRDED RMFTALWHRR RALIGRLFRL WFAQTERGKK RLEEERQQEE 

       190        200        210        220        230        240 
EEERKREARE RRRQLKRQLK EELESAPPRK SHRIRRQSLK ATSVKPARAP AKSTKSVRRR 

       250        260        270        280        290        300 
SAPARVSTPT PRTPRERTPQ QRPRPQAKAK RPQAPPSKTG DSPVKRRPTV TPGVTGLRNL 

       310        320        330        340        350        360 
GNTCYMNSIL QVLSHLHVFR ECFLRLDLNQ ALELLASAVS RKLGLSAQRV IQPKGLNQGS 

       370        380        390        400        410        420 
GLSGGASRSR NMELIQPKEP SSKHISLCHE LHTLFPVMWS GKWALVSPFA MLHSVWQLIP 

       430        440        450        460        470        480 
AFRGYAQQDA QEFLCELLDK VQHELERTRT LTPATVPANQ RRLIKQVLSV VNTIFHGQLL 

       490        500        510        520        530        540 
SQVRCLACDH RSNTIEPFWD LSLEFPERYH SNSKDAAQVP CGLTEMLAKF TETEALEGAI 

       550        560        570        580        590        600 
YACDYCNNKR RRFCSKQVVL TEAQKQLMVH KLPHVLRLHL KRFRWSGRNH REKIGVHVQF 

       610        620        630        640        650        660 
EQELNMEPYC CKDSGNLPHP QHFLYQLSAV VMHHGKGFGS GHYTAFCYNT EGGFWVHCND 

       670        680        690 
SKLSVCAVEE VCKAQAYILF YTQRNAQDKS KESDL 

« Hide

References

[1]NIH - Zebrafish Gene Collection (ZGC) project
Submitted (MAR-2003) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
Strain: AB.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
BC048060 mRNA. Translation: AAH48060.1.
RefSeqNP_956551.1. NM_200257.1.
UniGeneDr.78996.

3D structure databases

ProteinModelPortalQ7ZUM8.
ModBaseSearch...
MobiDBSearch...

Protein family/group databases

MEROPSC19.073.

Proteomic databases

PRIDEQ7ZUM8.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

GeneID393227.
KEGGdre:393227.

Organism-specific databases

CTD84101.
ZFINZDB-GENE-040426-774. usp44.

Phylogenomic databases

HOGENOMHOG000015084.
HOVERGENHBG018027.
InParanoidQ7ZUM8.
KOK11834.
PhylomeDBQ7ZUM8.

Family and domain databases

Gene3D3.30.40.10. 1 hit.
InterProIPR018200. Pept_C19ubi-hydrolase_C_CS.
IPR001394. Peptidase_C19_UCH.
IPR028889. UCH/PAN2.
IPR013083. Znf_RING/FYVE/PHD.
IPR001607. Znf_UBP.
[Graphical view]
PfamPF00443. UCH. 1 hit.
PF02148. zf-UBP. 1 hit.
[Graphical view]
SMARTSM00290. ZnF_UBP. 1 hit.
[Graphical view]
PROSITEPS00972. USP_1. 1 hit.
PS00973. USP_2. 1 hit.
PS50235. USP_3. 1 hit.
PS50271. ZF_UBP. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

NextBio20814291.
PROQ7ZUM8.

Entry information

Entry nameUBP44_DANRE
AccessionPrimary (citable) accession number: Q7ZUM8
Entry history
Integrated into UniProtKB/Swiss-Prot: July 13, 2010
Last sequence update: June 1, 2003
Last modified: April 16, 2014
This is version 65 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

Peptidase families

Classification of peptidase families and list of entries