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Q7ZUM8

- UBP44_DANRE

UniProt

Q7ZUM8 - UBP44_DANRE

Protein

Ubiquitin carboxyl-terminal hydrolase 44

Gene

usp44

Organism
Danio rerio (Zebrafish) (Brachydanio rerio)
Status
Reviewed - Annotation score: 4 out of 5- Experimental evidence at transcript leveli
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    • History
      Entry version 67 (01 Oct 2014)
      Sequence version 1 (01 Jun 2003)
      Previous versions | rss
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    Functioni

    Deubiquitinase that plays a key role in the spindle assembly checkpoint by preventing premature anaphase onset. Acts by specifically mediating deubiquitination of cdc20, a negative regulator of the anaphase promoting complex/cyclosome (APC/C) By similarity.By similarity

    Catalytic activityi

    Thiol-dependent hydrolysis of ester, thioester, amide, peptide and isopeptide bonds formed by the C-terminal Gly of ubiquitin (a 76-residue protein attached to proteins as an intracellular targeting signal).

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Active sitei304 – 3041NucleophilePROSITE-ProRule annotation
    Active sitei642 – 6421Proton acceptorPROSITE-ProRule annotation

    Regions

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Zinc fingeri24 – 8562UBP-typePROSITE-ProRule annotationAdd
    BLAST

    GO - Molecular functioni

    1. ubiquitin-specific protease activity Source: UniProtKB
    2. ubiquitin thiolesterase activity Source: UniProtKB
    3. zinc ion binding Source: InterPro

    GO - Biological processi

    1. anaphase Source: UniProtKB
    2. central nervous system morphogenesis Source: ZFIN
    3. mitotic nuclear division Source: UniProtKB-KW
    4. negative regulation of mitotic anaphase-promoting complex activity Source: UniProtKB
    5. protein deubiquitination Source: UniProtKB
    6. regulation of spindle checkpoint Source: UniProtKB
    7. ubiquitin-dependent protein catabolic process Source: InterPro

    Keywords - Molecular functioni

    Hydrolase, Protease, Thiol protease

    Keywords - Biological processi

    Cell cycle, Cell division, Mitosis, Ubl conjugation pathway

    Keywords - Ligandi

    Metal-binding, Zinc

    Protein family/group databases

    MEROPSiC19.073.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Ubiquitin carboxyl-terminal hydrolase 44 (EC:3.4.19.12)
    Alternative name(s):
    Deubiquitinating enzyme 44
    Ubiquitin thioesterase 44
    Ubiquitin-specific-processing protease 44
    Gene namesi
    Name:usp44
    ORF Names:zgc:55661
    OrganismiDanio rerio (Zebrafish) (Brachydanio rerio)
    Taxonomic identifieri7955 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiActinopterygiiNeopterygiiTeleosteiOstariophysiCypriniformesCyprinidaeDanio
    ProteomesiUP000000437: Unplaced

    Organism-specific databases

    ZFINiZDB-GENE-040426-774. usp44.

    Subcellular locationi

    Nucleus By similarity

    GO - Cellular componenti

    1. nucleus Source: UniProtKB

    Keywords - Cellular componenti

    Nucleus

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 695695Ubiquitin carboxyl-terminal hydrolase 44PRO_0000395812Add
    BLAST

    Proteomic databases

    PRIDEiQ7ZUM8.

    Structurei

    3D structure databases

    ProteinModelPortaliQ7ZUM8.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Domaini295 – 684390USPAdd
    BLAST

    Sequence similaritiesi

    Belongs to the peptidase C19 family. USP44 subfamily.Curated
    Contains 1 UBP-type zinc finger.PROSITE-ProRule annotation
    Contains 1 USP domain.Curated

    Zinc finger

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Zinc fingeri24 – 8562UBP-typePROSITE-ProRule annotationAdd
    BLAST

    Keywords - Domaini

    Zinc-finger

    Phylogenomic databases

    HOGENOMiHOG000015084.
    HOVERGENiHBG018027.
    InParanoidiQ7ZUM8.
    KOiK11834.
    PhylomeDBiQ7ZUM8.

    Family and domain databases

    Gene3Di3.30.40.10. 1 hit.
    InterProiIPR018200. Pept_C19ubi-hydrolase_C_CS.
    IPR001394. Peptidase_C19_UCH.
    IPR028889. UCH/PAN2.
    IPR013083. Znf_RING/FYVE/PHD.
    IPR001607. Znf_UBP.
    [Graphical view]
    PfamiPF00443. UCH. 1 hit.
    PF02148. zf-UBP. 1 hit.
    [Graphical view]
    SMARTiSM00290. ZnF_UBP. 1 hit.
    [Graphical view]
    PROSITEiPS00972. USP_1. 1 hit.
    PS00973. USP_2. 1 hit.
    PS50235. USP_3. 1 hit.
    PS50271. ZF_UBP. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Q7ZUM8-1 [UniParc]FASTAAdd to Basket

    « Hide

    MDRCKHVGRL RLAQDHSILN PQKWHCVDCN TTESVWACLS CSHVACGRYI    50
    EEHALQHFKE QHHPLALEVN ELYVYCYLCD DYVLNDNATG DLKLLRSTLS 100
    AIKSQCYEVT TRSGRTLRSS SANGDQLSPS TQELQLRDED RMFTALWHRR 150
    RALIGRLFRL WFAQTERGKK RLEEERQQEE EEERKREARE RRRQLKRQLK 200
    EELESAPPRK SHRIRRQSLK ATSVKPARAP AKSTKSVRRR SAPARVSTPT 250
    PRTPRERTPQ QRPRPQAKAK RPQAPPSKTG DSPVKRRPTV TPGVTGLRNL 300
    GNTCYMNSIL QVLSHLHVFR ECFLRLDLNQ ALELLASAVS RKLGLSAQRV 350
    IQPKGLNQGS GLSGGASRSR NMELIQPKEP SSKHISLCHE LHTLFPVMWS 400
    GKWALVSPFA MLHSVWQLIP AFRGYAQQDA QEFLCELLDK VQHELERTRT 450
    LTPATVPANQ RRLIKQVLSV VNTIFHGQLL SQVRCLACDH RSNTIEPFWD 500
    LSLEFPERYH SNSKDAAQVP CGLTEMLAKF TETEALEGAI YACDYCNNKR 550
    RRFCSKQVVL TEAQKQLMVH KLPHVLRLHL KRFRWSGRNH REKIGVHVQF 600
    EQELNMEPYC CKDSGNLPHP QHFLYQLSAV VMHHGKGFGS GHYTAFCYNT 650
    EGGFWVHCND SKLSVCAVEE VCKAQAYILF YTQRNAQDKS KESDL 695
    Length:695
    Mass (Da):79,926
    Last modified:June 1, 2003 - v1
    Checksum:iCEAE12134C67ED9F
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    BC048060 mRNA. Translation: AAH48060.1.
    RefSeqiNP_956551.1. NM_200257.1.
    UniGeneiDr.78996.

    Genome annotation databases

    GeneIDi393227.
    KEGGidre:393227.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    BC048060 mRNA. Translation: AAH48060.1 .
    RefSeqi NP_956551.1. NM_200257.1.
    UniGenei Dr.78996.

    3D structure databases

    ProteinModelPortali Q7ZUM8.
    ModBasei Search...
    MobiDBi Search...

    Protein family/group databases

    MEROPSi C19.073.

    Proteomic databases

    PRIDEi Q7ZUM8.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    GeneIDi 393227.
    KEGGi dre:393227.

    Organism-specific databases

    CTDi 84101.
    ZFINi ZDB-GENE-040426-774. usp44.

    Phylogenomic databases

    HOGENOMi HOG000015084.
    HOVERGENi HBG018027.
    InParanoidi Q7ZUM8.
    KOi K11834.
    PhylomeDBi Q7ZUM8.

    Miscellaneous databases

    NextBioi 20814291.
    PROi Q7ZUM8.

    Family and domain databases

    Gene3Di 3.30.40.10. 1 hit.
    InterProi IPR018200. Pept_C19ubi-hydrolase_C_CS.
    IPR001394. Peptidase_C19_UCH.
    IPR028889. UCH/PAN2.
    IPR013083. Znf_RING/FYVE/PHD.
    IPR001607. Znf_UBP.
    [Graphical view ]
    Pfami PF00443. UCH. 1 hit.
    PF02148. zf-UBP. 1 hit.
    [Graphical view ]
    SMARTi SM00290. ZnF_UBP. 1 hit.
    [Graphical view ]
    PROSITEi PS00972. USP_1. 1 hit.
    PS00973. USP_2. 1 hit.
    PS50235. USP_3. 1 hit.
    PS50271. ZF_UBP. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. NIH - Zebrafish Gene Collection (ZGC) project
      Submitted (MAR-2003) to the EMBL/GenBank/DDBJ databases
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
      Strain: AB.

    Entry informationi

    Entry nameiUBP44_DANRE
    AccessioniPrimary (citable) accession number: Q7ZUM8
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: July 13, 2010
    Last sequence update: June 1, 2003
    Last modified: October 1, 2014
    This is version 67 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. Peptidase families
      Classification of peptidase families and list of entries
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3