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Protein

Ubiquitin carboxyl-terminal hydrolase 44

Gene

usp44

Organism
Danio rerio (Zebrafish) (Brachydanio rerio)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at transcript leveli

Functioni

Deubiquitinase that plays a key role in the spindle assembly checkpoint by preventing premature anaphase onset. Acts by specifically mediating deubiquitination of cdc20, a negative regulator of the anaphase promoting complex/cyclosome (APC/C) (By similarity).By similarity

Catalytic activityi

Thiol-dependent hydrolysis of ester, thioester, amide, peptide and isopeptide bonds formed by the C-terminal Gly of ubiquitin (a 76-residue protein attached to proteins as an intracellular targeting signal).

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Active sitei304NucleophilePROSITE-ProRule annotation1
Active sitei642Proton acceptorPROSITE-ProRule annotation1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Zinc fingeri24 – 85UBP-typePROSITE-ProRule annotationAdd BLAST62

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Hydrolase, Protease, Thiol protease

Keywords - Biological processi

Cell cycle, Cell division, Mitosis, Ubl conjugation pathway

Keywords - Ligandi

Metal-binding, Zinc

Protein family/group databases

MEROPSiC19.057.

Names & Taxonomyi

Protein namesi
Recommended name:
Ubiquitin carboxyl-terminal hydrolase 44 (EC:3.4.19.12)
Alternative name(s):
Deubiquitinating enzyme 44
Ubiquitin thioesterase 44
Ubiquitin-specific-processing protease 44
Gene namesi
Name:usp44
ORF Names:zgc:55661
OrganismiDanio rerio (Zebrafish) (Brachydanio rerio)
Taxonomic identifieri7955 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiActinopterygiiNeopterygiiTeleosteiOstariophysiCypriniformesCyprinidaeDanio
Proteomesi
  • UP000000437 Componenti: Unplaced

Organism-specific databases

ZFINiZDB-GENE-040426-774. usp44.

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00003958121 – 695Ubiquitin carboxyl-terminal hydrolase 44Add BLAST695

Proteomic databases

PaxDbiQ7ZUM8.
PRIDEiQ7ZUM8.

Interactioni

Protein-protein interaction databases

STRINGi7955.ENSDARP00000104741.

Structurei

3D structure databases

ProteinModelPortaliQ7ZUM8.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini295 – 684USPAdd BLAST390

Sequence similaritiesi

Belongs to the peptidase C19 family. USP44 subfamily.Curated
Contains 1 UBP-type zinc finger.PROSITE-ProRule annotation
Contains 1 USP domain.Curated

Zinc finger

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Zinc fingeri24 – 85UBP-typePROSITE-ProRule annotationAdd BLAST62

Keywords - Domaini

Zinc-finger

Phylogenomic databases

eggNOGiKOG1867. Eukaryota.
ENOG410XQQ0. LUCA.
HOGENOMiHOG000015084.
HOVERGENiHBG018027.
InParanoidiQ7ZUM8.
KOiK11834.
PhylomeDBiQ7ZUM8.

Family and domain databases

Gene3Di3.30.40.10. 1 hit.
InterProiIPR001394. Peptidase_C19_UCH.
IPR018200. USP_CS.
IPR028889. USP_dom.
IPR013083. Znf_RING/FYVE/PHD.
IPR001607. Znf_UBP.
[Graphical view]
PfamiPF00443. UCH. 1 hit.
PF02148. zf-UBP. 1 hit.
[Graphical view]
SMARTiSM00290. ZnF_UBP. 1 hit.
[Graphical view]
PROSITEiPS00972. USP_1. 1 hit.
PS00973. USP_2. 1 hit.
PS50235. USP_3. 1 hit.
PS50271. ZF_UBP. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q7ZUM8-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MDRCKHVGRL RLAQDHSILN PQKWHCVDCN TTESVWACLS CSHVACGRYI
60 70 80 90 100
EEHALQHFKE QHHPLALEVN ELYVYCYLCD DYVLNDNATG DLKLLRSTLS
110 120 130 140 150
AIKSQCYEVT TRSGRTLRSS SANGDQLSPS TQELQLRDED RMFTALWHRR
160 170 180 190 200
RALIGRLFRL WFAQTERGKK RLEEERQQEE EEERKREARE RRRQLKRQLK
210 220 230 240 250
EELESAPPRK SHRIRRQSLK ATSVKPARAP AKSTKSVRRR SAPARVSTPT
260 270 280 290 300
PRTPRERTPQ QRPRPQAKAK RPQAPPSKTG DSPVKRRPTV TPGVTGLRNL
310 320 330 340 350
GNTCYMNSIL QVLSHLHVFR ECFLRLDLNQ ALELLASAVS RKLGLSAQRV
360 370 380 390 400
IQPKGLNQGS GLSGGASRSR NMELIQPKEP SSKHISLCHE LHTLFPVMWS
410 420 430 440 450
GKWALVSPFA MLHSVWQLIP AFRGYAQQDA QEFLCELLDK VQHELERTRT
460 470 480 490 500
LTPATVPANQ RRLIKQVLSV VNTIFHGQLL SQVRCLACDH RSNTIEPFWD
510 520 530 540 550
LSLEFPERYH SNSKDAAQVP CGLTEMLAKF TETEALEGAI YACDYCNNKR
560 570 580 590 600
RRFCSKQVVL TEAQKQLMVH KLPHVLRLHL KRFRWSGRNH REKIGVHVQF
610 620 630 640 650
EQELNMEPYC CKDSGNLPHP QHFLYQLSAV VMHHGKGFGS GHYTAFCYNT
660 670 680 690
EGGFWVHCND SKLSVCAVEE VCKAQAYILF YTQRNAQDKS KESDL
Length:695
Mass (Da):79,926
Last modified:June 1, 2003 - v1
Checksum:iCEAE12134C67ED9F
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
BC048060 mRNA. Translation: AAH48060.1.
RefSeqiNP_956551.1. NM_200257.1.
UniGeneiDr.78996.

Genome annotation databases

GeneIDi393227.
KEGGidre:393227.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
BC048060 mRNA. Translation: AAH48060.1.
RefSeqiNP_956551.1. NM_200257.1.
UniGeneiDr.78996.

3D structure databases

ProteinModelPortaliQ7ZUM8.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi7955.ENSDARP00000104741.

Protein family/group databases

MEROPSiC19.057.

Proteomic databases

PaxDbiQ7ZUM8.
PRIDEiQ7ZUM8.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

GeneIDi393227.
KEGGidre:393227.

Organism-specific databases

CTDi84101.
ZFINiZDB-GENE-040426-774. usp44.

Phylogenomic databases

eggNOGiKOG1867. Eukaryota.
ENOG410XQQ0. LUCA.
HOGENOMiHOG000015084.
HOVERGENiHBG018027.
InParanoidiQ7ZUM8.
KOiK11834.
PhylomeDBiQ7ZUM8.

Miscellaneous databases

PROiQ7ZUM8.

Family and domain databases

Gene3Di3.30.40.10. 1 hit.
InterProiIPR001394. Peptidase_C19_UCH.
IPR018200. USP_CS.
IPR028889. USP_dom.
IPR013083. Znf_RING/FYVE/PHD.
IPR001607. Znf_UBP.
[Graphical view]
PfamiPF00443. UCH. 1 hit.
PF02148. zf-UBP. 1 hit.
[Graphical view]
SMARTiSM00290. ZnF_UBP. 1 hit.
[Graphical view]
PROSITEiPS00972. USP_1. 1 hit.
PS00973. USP_2. 1 hit.
PS50235. USP_3. 1 hit.
PS50271. ZF_UBP. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiUBP44_DANRE
AccessioniPrimary (citable) accession number: Q7ZUM8
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 13, 2010
Last sequence update: June 1, 2003
Last modified: July 6, 2016
This is version 78 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Peptidase families
    Classification of peptidase families and list of entries
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.