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Protein

Transitional endoplasmic reticulum ATPase

Gene

vcp

Organism
Danio rerio (Zebrafish) (Brachydanio rerio)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Necessary for the fragmentation of Golgi stacks during mitosis and for their reassembly after mitosis. Involved in the formation of the nuclear envelope, and of the transitional endoplasmic reticulum (tER). The transfer of membranes from the endoplasmic reticulum to the Golgi apparatus occurs via 50-70 nm transition vesicles which derive from part-rough, part-smooth transitional elements of the endoplasmic reticulum (tER). Vesicle budding from the tER is an ATP-dependent process. Also involved in DNA damage response: recruited to double-strand breaks (DSBs) sites and promotes the recruitment of tp53bp1 at DNA damage sites (By similarity). Enhances cell cycle progression and inhibits apoptosis at low temperatures (PubMed:12914916). Essential for the maturation of ubiquitin-containing autophagosomes and the clearance of ubiquitinated protein by autophagy (By similarity). Acts as a negative regulator of type I interferon production by promoting ubiquitination of ddx58/rig-i (By similarity). May play a role in the ubiquitin-dependent sorting of membrane proteins to lysosomes where they undergo degradation (By similarity).By similarity1 Publication

Catalytic activityi

ATP + H2O = ADP + phosphate.By similarity

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei348ATPBy similarity1
Binding sitei384ATPBy similarity1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi247 – 253ATPBy similarity7
Nucleotide bindingi521 – 526ATPBy similarity6

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionHydrolase
Biological processAutophagy, Cell cycle, DNA damage, DNA repair, Transport
LigandATP-binding, Lipid-binding, Nucleotide-binding

Enzyme and pathway databases

ReactomeiR-DRE-110320. Translesion Synthesis by POLH.
R-DRE-3371511. HSF1 activation.
R-DRE-382556. ABC-family proteins mediated transport.
R-DRE-532668. N-glycan trimming in the ER and Calnexin/Calreticulin cycle.
R-DRE-5358346. Hedgehog ligand biogenesis.
R-DRE-5689877. Josephin domain DUBs.
R-DRE-5689896. Ovarian tumor domain proteases.
R-DRE-6798695. Neutrophil degranulation.
R-DRE-8876725. Protein methylation.

Names & Taxonomyi

Protein namesi
Recommended name:
Transitional endoplasmic reticulum ATPaseBy similarity (EC:3.6.4.6By similarity)
Short name:
TER ATPaseBy similarity
Alternative name(s):
Protein CDC481 PublicationImported
Valosin-containing protein1 PublicationImported
Short name:
VCP1 PublicationBy similarity
Gene namesi
Name:vcpImported
Synonyms:cdc48
ORF Names:si:ch211-113n10.2
OrganismiDanio rerio (Zebrafish) (Brachydanio rerio)
Taxonomic identifieri7955 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiActinopterygiiNeopterygiiTeleosteiOstariophysiCypriniformesCyprinidaeDanio
Proteomesi
  • UP000000437 Componenti: Chromosome 5

Organism-specific databases

ZFINiZDB-GENE-030131-5408. vcp.

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Cytoplasm, Endoplasmic reticulum, Nucleus

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi805Y → A: Inhibits cell-proliferation and enhances apoptosis at low temperatures. 1 Publication1

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00003822331 – 806Transitional endoplasmic reticulum ATPaseAdd BLAST806

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei3Phosphoserine1 Publication1

Keywords - PTMi

Phosphoprotein

Proteomic databases

PaxDbiQ7ZU99.
PRIDEiQ7ZU99.

PTM databases

iPTMnetiQ7ZU99.

Expressioni

Inductioni

By cold.1 Publication

Gene expression databases

BgeeiENSDARG00000020008.

Interactioni

Subunit structurei

Homohexamer.By similarity

Protein-protein interaction databases

IntActiQ7ZU99. 1 interactor.
MINTiMINT-8283632.
STRINGi7955.ENSDARP00000012048.

Structurei

3D structure databases

ProteinModelPortaliQ7ZU99.
SMRiQ7ZU99.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the AAA ATPase family.Sequence analysis

Phylogenomic databases

eggNOGiKOG0730. Eukaryota.
COG0464. LUCA.
GeneTreeiENSGT00890000139420.
HOGENOMiHOG000223224.
InParanoidiQ7ZU99.
KOiK13525.
OMAiPIDDTTE.
OrthoDBiEOG091G024K.
PhylomeDBiQ7ZU99.
TreeFamiTF300542.

Family and domain databases

Gene3Di3.10.330.10. 1 hit.
InterProiView protein in InterPro
IPR003593. AAA+_ATPase.
IPR005938. AAA_ATPase_CDC48.
IPR009010. Asp_de-COase-like_dom.
IPR003959. ATPase_AAA_core.
IPR003960. ATPase_AAA_CS.
IPR004201. Cdc48_dom2.
IPR029067. CDC48_domain_2-like.
IPR003338. CDC4_N-term_subdom.
IPR027417. P-loop_NTPase.
IPR015415. Vps4_C.
PfamiView protein in Pfam
PF00004. AAA. 2 hits.
PF02933. CDC48_2. 1 hit.
PF02359. CDC48_N. 1 hit.
PF09336. Vps4_C. 1 hit.
SMARTiView protein in SMART
SM00382. AAA. 2 hits.
SM01072. CDC48_2. 1 hit.
SM01073. CDC48_N. 1 hit.
SUPFAMiSSF50692. SSF50692. 1 hit.
SSF52540. SSF52540. 2 hits.
SSF54585. SSF54585. 1 hit.
TIGRFAMsiTIGR01243. CDC48. 1 hit.
PROSITEiView protein in PROSITE
PS00674. AAA. 2 hits.

Sequencei

Sequence statusi: Complete.

Q7ZU99-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MASGGESKND DLSTAILKQK NRPNRLIVDE SINEDNSVVS LSQAKMDELQ
60 70 80 90 100
LFRGDTVLLK GKKRRETVCI VLSDDTCSDE KVRMNRVVRN NLRVRLGDVI
110 120 130 140 150
SIQPCPDVKY GKRIHVLPID DTVEGITGNL FEVYLKPYFL EAYRPIRKGD
160 170 180 190 200
IFLVRGGMRA VEFKVVETDP SPYCIVAPDT VIHCEGEPIK REDEEESLNE
210 220 230 240 250
VGYDDIGGVR KQLAQIKEMV ELPLRHPALF KAIGVKPPRG ILLYGPPGTG
260 270 280 290 300
KTLIARAVAN ETGAFFFLIN GPEIMSKLAG ESESNLRKAF EEAEKNAPAI
310 320 330 340 350
IFIDELDAIA PKREKTHGEV ERRIVSQLLT LMDGLKQRAH VIVMAATNRP
360 370 380 390 400
NSIDPALRRF GRFDREVDIG IPDATGRLEI LQIHTKNMKL ADDVDLEQVA
410 420 430 440 450
NETHGHVGAD LAALCSEAAL QAIRKKMDLI DLEDETIDAE VMNSLAVTMD
460 470 480 490 500
DFRWALSQSN PSALRETVVE VPNITWEDIG GLDDVKRELQ ELVQYPVEHP
510 520 530 540 550
DKFLKFGMTP SKGVLFYGPP GCGKTLLAKA IANECQANFI SIKGPELLTM
560 570 580 590 600
WFGESEANVR EIFDKARQAA PCVLFFDELD SIAKARGGNV GDGGGAADRV
610 620 630 640 650
INQILTEMDG MSSKKNVFII GATNRPDIID PAILRPGRLD QLIYIPLPDE
660 670 680 690 700
KSRIAILKAN LRKSPISKDV DLDFLAKMTN GFSGADLTEI CQRACKLAIR
710 720 730 740 750
ESIENEIRRE RERQTNPSAM EVEEDDPVPE IRKDHFEEAM RFARRSVSDN
760 770 780 790 800
DIRKYEMFAQ TLQQSRGFGS FRFPSSNQGG SGPSQGSSGG GGGNVFNEDN

DDDLYG
Length:806
Mass (Da):89,424
Last modified:June 1, 2003 - v1
Checksum:iE126C61DFD7EE174
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti641 – 643QLI → HIM in BAC87740 (PubMed:12914916).Curated3
Sequence conflicti709R → L in BAC87740 (PubMed:12914916).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AB093594 mRNA. Translation: BAC87740.1.
AY576993 mRNA. Translation: AAS92631.1.
CR318632 Genomic DNA. Translation: CAM13143.1.
BC050488 mRNA. Translation: AAH50488.1.
BC067384 mRNA. Translation: AAH67384.1.
RefSeqiNP_958889.1. NM_201481.1.
UniGeneiDr.75122.

Genome annotation databases

EnsembliENSDART00000023779; ENSDARP00000012048; ENSDARG00000020008.
GeneIDi327197.
KEGGidre:327197.

Similar proteinsi

Entry informationi

Entry nameiTERA_DANRE
AccessioniPrimary (citable) accession number: Q7ZU99
Secondary accession number(s): Q76KA4
Entry historyiIntegrated into UniProtKB/Swiss-Prot: September 1, 2009
Last sequence update: June 1, 2003
Last modified: September 27, 2017
This is version 131 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families