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Q7ZU99

- TERA_DANRE

UniProt

Q7ZU99 - TERA_DANRE

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Protein

Transitional endoplasmic reticulum ATPase

Gene

vcp

Organism
Danio rerio (Zebrafish) (Brachydanio rerio)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli

Functioni

Necessary for the fragmentation of Golgi stacks during mitosis and for their reassembly after mitosis. Involved in the formation of the nuclear envelope, and of the transitional endoplasmic reticulum (tER). The transfer of membranes from the endoplasmic reticulum to the Golgi apparatus occurs via 50-70 nm transition vesicles which derive from part-rough, part-smooth transitional elements of the endoplasmic reticulum (tER). Vesicle budding from the tER is an ATP-dependent process (By similarity). Enhances cell cycle progression and inhibits apoptosis at low temperatures. Also involved in DNA damage response: recruited to double-strand breaks (DSBs) sites and promotes the recruitment of tp53bp1 at DNA damage sites.By similarity1 Publication

Catalytic activityi

ATP + H2O = ADP + phosphate.By similarity

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei348 – 3481ATPBy similarity
Binding sitei384 – 3841ATPBy similarity

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi247 – 2537ATPBy similarity

GO - Molecular functioni

  1. ATP binding Source: UniProtKB-KW
  2. hydrolase activity Source: UniProtKB-KW
  3. lipid binding Source: UniProtKB-KW

GO - Biological processi

  1. cell cycle Source: UniProtKB-KW
  2. cellular response to DNA damage stimulus Source: UniProtKB
  3. double-strand break repair Source: UniProtKB
  4. ER-associated ubiquitin-dependent protein catabolic process Source: UniProtKB
  5. protein N-linked glycosylation via asparagine Source: UniProtKB
  6. protein ubiquitination Source: UniProtKB
  7. regulation of protein ubiquitination involved in ubiquitin-dependent protein catabolic process Source: ZFIN
  8. translesion synthesis Source: UniProtKB
  9. transport Source: UniProtKB-KW
Complete GO annotation...

Keywords - Molecular functioni

Hydrolase

Keywords - Biological processi

Cell cycle, DNA damage, DNA repair, Transport

Keywords - Ligandi

ATP-binding, Lipid-binding, Nucleotide-binding

Enzyme and pathway databases

ReactomeiREACT_225331. HSF1 activation.

Names & Taxonomyi

Protein namesi
Recommended name:
Transitional endoplasmic reticulum ATPaseBy similarity (EC:3.6.4.6)
Short name:
TER ATPaseBy similarity
Alternative name(s):
Protein CDC48Imported1 Publication
Valosin-containing proteinImported1 Publication
Short name:
VCPBy similarity1 Publication
Gene namesi
Name:vcpImported
Synonyms:cdc48
ORF Names:si:ch211-113n10.2
OrganismiDanio rerio (Zebrafish) (Brachydanio rerio)
Taxonomic identifieri7955 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiActinopterygiiNeopterygiiTeleosteiOstariophysiCypriniformesCyprinidaeDanio
ProteomesiUP000000437: Chromosome 5

Organism-specific databases

ZFINiZDB-GENE-030131-5408. vcp.

Subcellular locationi

Cytoplasmcytosol By similarity. Nucleus By similarity
Note: Following DNA double-strand breaks, recruited to the sites of damage.By similarity

GO - Cellular componenti

  1. cytoplasm Source: UniProtKB-KW
  2. nucleus Source: UniProtKB-KW
  3. site of double-strand break Source: UniProtKB
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm, Nucleus

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Mutagenesisi805 – 8051Y → A: Inhibits cell-proliferation and enhances apoptosis at low temperatures. 1 Publication

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 806806Transitional endoplasmic reticulum ATPasePRO_0000382233Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei3 – 31Phosphoserine1 Publication

Keywords - PTMi

Phosphoprotein

Proteomic databases

PRIDEiQ7ZU99.

Expressioni

Inductioni

By cold.1 Publication

Gene expression databases

BgeeiQ7ZU99.

Interactioni

Subunit structurei

Homohexamer.By similarity

Protein-protein interaction databases

IntActiQ7ZU99. 1 interaction.
MINTiMINT-8283632.
STRINGi7955.ENSDARP00000012048.

Structurei

3D structure databases

ProteinModelPortaliQ7ZU99.
SMRiQ7ZU99. Positions 18-763.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the AAA ATPase family.Sequence Analysis

Phylogenomic databases

eggNOGiCOG0464.
GeneTreeiENSGT00740000115575.
HOGENOMiHOG000223224.
InParanoidiQ7ZU99.
KOiK13525.
OMAiEIFDKPC.
OrthoDBiEOG7H4DSW.
PhylomeDBiQ7ZU99.
TreeFamiTF300542.

Family and domain databases

Gene3Di3.10.330.10. 1 hit.
3.40.50.300. 2 hits.
InterProiIPR003593. AAA+_ATPase.
IPR005938. AAA_ATPase_CDC48.
IPR009010. Asp_de-COase-like_dom.
IPR003959. ATPase_AAA_core.
IPR003960. ATPase_AAA_CS.
IPR004201. Cdc48_dom2.
IPR029067. CDC48_domain_2-like.
IPR003338. CDC4_N-term_subdom.
IPR027417. P-loop_NTPase.
IPR015415. Vps4_C.
[Graphical view]
PfamiPF00004. AAA. 2 hits.
PF02933. CDC48_2. 1 hit.
PF02359. CDC48_N. 1 hit.
PF09336. Vps4_C. 1 hit.
[Graphical view]
SMARTiSM00382. AAA. 2 hits.
SM01072. CDC48_2. 1 hit.
SM01073. CDC48_N. 1 hit.
[Graphical view]
SUPFAMiSSF50692. SSF50692. 1 hit.
SSF52540. SSF52540. 2 hits.
SSF54585. SSF54585. 1 hit.
TIGRFAMsiTIGR01243. CDC48. 1 hit.
PROSITEiPS00674. AAA. 2 hits.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q7ZU99 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MASGGESKND DLSTAILKQK NRPNRLIVDE SINEDNSVVS LSQAKMDELQ
60 70 80 90 100
LFRGDTVLLK GKKRRETVCI VLSDDTCSDE KVRMNRVVRN NLRVRLGDVI
110 120 130 140 150
SIQPCPDVKY GKRIHVLPID DTVEGITGNL FEVYLKPYFL EAYRPIRKGD
160 170 180 190 200
IFLVRGGMRA VEFKVVETDP SPYCIVAPDT VIHCEGEPIK REDEEESLNE
210 220 230 240 250
VGYDDIGGVR KQLAQIKEMV ELPLRHPALF KAIGVKPPRG ILLYGPPGTG
260 270 280 290 300
KTLIARAVAN ETGAFFFLIN GPEIMSKLAG ESESNLRKAF EEAEKNAPAI
310 320 330 340 350
IFIDELDAIA PKREKTHGEV ERRIVSQLLT LMDGLKQRAH VIVMAATNRP
360 370 380 390 400
NSIDPALRRF GRFDREVDIG IPDATGRLEI LQIHTKNMKL ADDVDLEQVA
410 420 430 440 450
NETHGHVGAD LAALCSEAAL QAIRKKMDLI DLEDETIDAE VMNSLAVTMD
460 470 480 490 500
DFRWALSQSN PSALRETVVE VPNITWEDIG GLDDVKRELQ ELVQYPVEHP
510 520 530 540 550
DKFLKFGMTP SKGVLFYGPP GCGKTLLAKA IANECQANFI SIKGPELLTM
560 570 580 590 600
WFGESEANVR EIFDKARQAA PCVLFFDELD SIAKARGGNV GDGGGAADRV
610 620 630 640 650
INQILTEMDG MSSKKNVFII GATNRPDIID PAILRPGRLD QLIYIPLPDE
660 670 680 690 700
KSRIAILKAN LRKSPISKDV DLDFLAKMTN GFSGADLTEI CQRACKLAIR
710 720 730 740 750
ESIENEIRRE RERQTNPSAM EVEEDDPVPE IRKDHFEEAM RFARRSVSDN
760 770 780 790 800
DIRKYEMFAQ TLQQSRGFGS FRFPSSNQGG SGPSQGSSGG GGGNVFNEDN

DDDLYG
Length:806
Mass (Da):89,424
Last modified:June 1, 2003 - v1
Checksum:iE126C61DFD7EE174
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti641 – 6433QLI → HIM in BAC87740. (PubMed:12914916)Curated
Sequence conflicti709 – 7091R → L in BAC87740. (PubMed:12914916)Curated

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AB093594 mRNA. Translation: BAC87740.1.
AY576993 mRNA. Translation: AAS92631.1.
CR318632 Genomic DNA. Translation: CAM13143.1.
BC050488 mRNA. Translation: AAH50488.1.
BC067384 mRNA. Translation: AAH67384.1.
RefSeqiNP_958889.1. NM_201481.1.
UniGeneiDr.75122.

Genome annotation databases

EnsembliENSDART00000023779; ENSDARP00000012048; ENSDARG00000020008.
GeneIDi327197.
KEGGidre:327197.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AB093594 mRNA. Translation: BAC87740.1 .
AY576993 mRNA. Translation: AAS92631.1 .
CR318632 Genomic DNA. Translation: CAM13143.1 .
BC050488 mRNA. Translation: AAH50488.1 .
BC067384 mRNA. Translation: AAH67384.1 .
RefSeqi NP_958889.1. NM_201481.1.
UniGenei Dr.75122.

3D structure databases

ProteinModelPortali Q7ZU99.
SMRi Q7ZU99. Positions 18-763.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

IntActi Q7ZU99. 1 interaction.
MINTi MINT-8283632.
STRINGi 7955.ENSDARP00000012048.

Proteomic databases

PRIDEi Q7ZU99.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

Ensembli ENSDART00000023779 ; ENSDARP00000012048 ; ENSDARG00000020008 .
GeneIDi 327197.
KEGGi dre:327197.

Organism-specific databases

CTDi 7415.
ZFINi ZDB-GENE-030131-5408. vcp.

Phylogenomic databases

eggNOGi COG0464.
GeneTreei ENSGT00740000115575.
HOGENOMi HOG000223224.
InParanoidi Q7ZU99.
KOi K13525.
OMAi EIFDKPC.
OrthoDBi EOG7H4DSW.
PhylomeDBi Q7ZU99.
TreeFami TF300542.

Enzyme and pathway databases

Reactomei REACT_225331. HSF1 activation.

Miscellaneous databases

NextBioi 20809924.
PROi Q7ZU99.

Gene expression databases

Bgeei Q7ZU99.

Family and domain databases

Gene3Di 3.10.330.10. 1 hit.
3.40.50.300. 2 hits.
InterProi IPR003593. AAA+_ATPase.
IPR005938. AAA_ATPase_CDC48.
IPR009010. Asp_de-COase-like_dom.
IPR003959. ATPase_AAA_core.
IPR003960. ATPase_AAA_CS.
IPR004201. Cdc48_dom2.
IPR029067. CDC48_domain_2-like.
IPR003338. CDC4_N-term_subdom.
IPR027417. P-loop_NTPase.
IPR015415. Vps4_C.
[Graphical view ]
Pfami PF00004. AAA. 2 hits.
PF02933. CDC48_2. 1 hit.
PF02359. CDC48_N. 1 hit.
PF09336. Vps4_C. 1 hit.
[Graphical view ]
SMARTi SM00382. AAA. 2 hits.
SM01072. CDC48_2. 1 hit.
SM01073. CDC48_N. 1 hit.
[Graphical view ]
SUPFAMi SSF50692. SSF50692. 1 hit.
SSF52540. SSF52540. 2 hits.
SSF54585. SSF54585. 1 hit.
TIGRFAMsi TIGR01243. CDC48. 1 hit.
PROSITEi PS00674. AAA. 2 hits.
[Graphical view ]
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "Cold-inducible expression of the cell division cycle gene CDC48 and its promotion of cell proliferation during cold acclimation in zebrafish cells."
    Imamura S., Ojima N., Yamashita M.
    FEBS Lett. 549:14-20(2003) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA], FUNCTION, INDUCTION, MUTAGENESIS OF TYR-805.
    Tissue: Embryo1 Publication.
  2. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Tissue: Kidney marrow1 Publication.
  3. "The zebrafish reference genome sequence and its relationship to the human genome."
    Howe K., Clark M.D., Torroja C.F., Torrance J., Berthelot C., Muffato M., Collins J.E., Humphray S., McLaren K., Matthews L., McLaren S., Sealy I., Caccamo M., Churcher C., Scott C., Barrett J.C., Koch R., Rauch G.J.
    , White S., Chow W., Kilian B., Quintais L.T., Guerra-Assuncao J.A., Zhou Y., Gu Y., Yen J., Vogel J.H., Eyre T., Redmond S., Banerjee R., Chi J., Fu B., Langley E., Maguire S.F., Laird G.K., Lloyd D., Kenyon E., Donaldson S., Sehra H., Almeida-King J., Loveland J., Trevanion S., Jones M., Quail M., Willey D., Hunt A., Burton J., Sims S., McLay K., Plumb B., Davis J., Clee C., Oliver K., Clark R., Riddle C., Eliott D., Threadgold G., Harden G., Ware D., Mortimer B., Kerry G., Heath P., Phillimore B., Tracey A., Corby N., Dunn M., Johnson C., Wood J., Clark S., Pelan S., Griffiths G., Smith M., Glithero R., Howden P., Barker N., Stevens C., Harley J., Holt K., Panagiotidis G., Lovell J., Beasley H., Henderson C., Gordon D., Auger K., Wright D., Collins J., Raisen C., Dyer L., Leung K., Robertson L., Ambridge K., Leongamornlert D., McGuire S., Gilderthorp R., Griffiths C., Manthravadi D., Nichol S., Barker G., Whitehead S., Kay M., Brown J., Murnane C., Gray E., Humphries M., Sycamore N., Barker D., Saunders D., Wallis J., Babbage A., Hammond S., Mashreghi-Mohammadi M., Barr L., Martin S., Wray P., Ellington A., Matthews N., Ellwood M., Woodmansey R., Clark G., Cooper J., Tromans A., Grafham D., Skuce C., Pandian R., Andrews R., Harrison E., Kimberley A., Garnett J., Fosker N., Hall R., Garner P., Kelly D., Bird C., Palmer S., Gehring I., Berger A., Dooley C.M., Ersan-Urun Z., Eser C., Geiger H., Geisler M., Karotki L., Kirn A., Konantz J., Konantz M., Oberlander M., Rudolph-Geiger S., Teucke M., Osoegawa K., Zhu B., Rapp A., Widaa S., Langford C., Yang F., Carter N.P., Harrow J., Ning Z., Herrero J., Searle S.M., Enright A., Geisler R., Plasterk R.H., Lee C., Westerfield M., de Jong P.J., Zon L.I., Postlethwait J.H., Nusslein-Volhard C., Hubbard T.J., Roest Crollius H., Rogers J., Stemple D.L.
    Nature 496:498-503(2013) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: Tuebingen.
  4. NIH - Zebrafish Gene Collection (ZGC) project
    Submitted (MAR-2004) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Strain: ABImported.
    Tissue: KidneyImported.
  5. "Online automated in vivo zebrafish phosphoproteomics: from large-scale analysis down to a single embryo."
    Lemeer S., Pinkse M.W.H., Mohammed S., van Breukelen B., den Hertog J., Slijper M., Heck A.J.R.
    J. Proteome Res. 7:1555-1564(2008) [PubMed] [Europe PMC] [Abstract]
    Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-3, IDENTIFICATION BY MASS SPECTROMETRY.
    Tissue: Embryo1 Publication.

Entry informationi

Entry nameiTERA_DANRE
AccessioniPrimary (citable) accession number: Q7ZU99
Secondary accession number(s): Q76KA4
Entry historyi
Integrated into UniProtKB/Swiss-Prot: September 1, 2009
Last sequence update: June 1, 2003
Last modified: October 29, 2014
This is version 109 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3