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Protein

Transitional endoplasmic reticulum ATPase

Gene

vcp

Organism
Danio rerio (Zebrafish) (Brachydanio rerio)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Necessary for the fragmentation of Golgi stacks during mitosis and for their reassembly after mitosis. Involved in the formation of the nuclear envelope, and of the transitional endoplasmic reticulum (tER). The transfer of membranes from the endoplasmic reticulum to the Golgi apparatus occurs via 50-70 nm transition vesicles which derive from part-rough, part-smooth transitional elements of the endoplasmic reticulum (tER). Vesicle budding from the tER is an ATP-dependent process. Also involved in DNA damage response: recruited to double-strand breaks (DSBs) sites and promotes the recruitment of tp53bp1 at DNA damage sites (By similarity). Enhances cell cycle progression and inhibits apoptosis at low temperatures (PubMed:12914916). Essential for the maturation of ubiquitin-containing autophagosomes and the clearance of ubiquitinated protein by autophagy (By similarity). Acts as a negative regulator of type I interferon production by promoting ubiquitination of ddx58/rig-i (By similarity). May play a role in the ubiquitin-dependent sorting of membrane proteins to lysosomes where they undergo degradation (By similarity).By similarity1 Publication

Catalytic activityi

ATP + H2O = ADP + phosphate.By similarity

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei348ATPBy similarity1
Binding sitei384ATPBy similarity1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi247 – 253ATPBy similarity7
Nucleotide bindingi521 – 526ATPBy similarity6

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionHydrolase
Biological processAutophagy, Cell cycle, DNA damage, DNA repair, Transport
LigandATP-binding, Lipid-binding, Nucleotide-binding

Enzyme and pathway databases

ReactomeiR-DRE-110320 Translesion Synthesis by POLH
R-DRE-3371511 HSF1 activation
R-DRE-382556 ABC-family proteins mediated transport
R-DRE-532668 N-glycan trimming in the ER and Calnexin/Calreticulin cycle
R-DRE-5358346 Hedgehog ligand biogenesis
R-DRE-5689877 Josephin domain DUBs
R-DRE-5689896 Ovarian tumor domain proteases
R-DRE-6798695 Neutrophil degranulation
R-DRE-8876725 Protein methylation

Names & Taxonomyi

Protein namesi
Recommended name:
Transitional endoplasmic reticulum ATPaseBy similarity (EC:3.6.4.6By similarity)
Short name:
TER ATPaseBy similarity
Alternative name(s):
Protein CDC481 PublicationImported
Valosin-containing protein1 PublicationImported
Short name:
VCP1 PublicationBy similarity
Gene namesi
Name:vcpImported
Synonyms:cdc48
ORF Names:si:ch211-113n10.2
OrganismiDanio rerio (Zebrafish) (Brachydanio rerio)
Taxonomic identifieri7955 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiActinopterygiiNeopterygiiTeleosteiOstariophysiCypriniformesCyprinidaeDanio
Proteomesi
  • UP000000437 Componenti: Chromosome 5

Organism-specific databases

ZFINiZDB-GENE-030131-5408 vcp

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Cytoplasm, Endoplasmic reticulum, Nucleus

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi805Y → A: Inhibits cell-proliferation and enhances apoptosis at low temperatures. 1 Publication1

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00003822331 – 806Transitional endoplasmic reticulum ATPaseAdd BLAST806

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei3Phosphoserine1 Publication1

Keywords - PTMi

Phosphoprotein

Proteomic databases

PaxDbiQ7ZU99
PRIDEiQ7ZU99

PTM databases

iPTMnetiQ7ZU99

Expressioni

Inductioni

By cold.1 Publication

Gene expression databases

BgeeiENSDARG00000020008

Interactioni

Subunit structurei

Homohexamer.By similarity

Protein-protein interaction databases

IntActiQ7ZU99 1 interactor.
MINTiQ7ZU99
STRINGi7955.ENSDARP00000012048

Structurei

3D structure databases

ProteinModelPortaliQ7ZU99
SMRiQ7ZU99
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the AAA ATPase family.Sequence analysis

Phylogenomic databases

eggNOGiKOG0730 Eukaryota
COG0464 LUCA
GeneTreeiENSGT00900000141071
HOGENOMiHOG000223224
InParanoidiQ7ZU99
KOiK13525
OMAiPIDDTTE
OrthoDBiEOG091G024K
PhylomeDBiQ7ZU99
TreeFamiTF300542

Family and domain databases

InterProiView protein in InterPro
IPR003593 AAA+_ATPase
IPR005938 AAA_ATPase_CDC48
IPR009010 Asp_de-COase-like_dom_sf
IPR003959 ATPase_AAA_core
IPR003960 ATPase_AAA_CS
IPR004201 Cdc48_dom2
IPR029067 CDC48_domain_2-like_sf
IPR003338 CDC4_N-term_subdom
IPR027417 P-loop_NTPase
IPR015415 Vps4_C
PfamiView protein in Pfam
PF00004 AAA, 2 hits
PF02933 CDC48_2, 1 hit
PF02359 CDC48_N, 1 hit
PF09336 Vps4_C, 1 hit
SMARTiView protein in SMART
SM00382 AAA, 2 hits
SM01072 CDC48_2, 1 hit
SM01073 CDC48_N, 1 hit
SUPFAMiSSF50692 SSF50692, 1 hit
SSF52540 SSF52540, 2 hits
SSF54585 SSF54585, 1 hit
TIGRFAMsiTIGR01243 CDC48, 1 hit
PROSITEiView protein in PROSITE
PS00674 AAA, 2 hits

Sequencei

Sequence statusi: Complete.

Q7ZU99-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MASGGESKND DLSTAILKQK NRPNRLIVDE SINEDNSVVS LSQAKMDELQ
60 70 80 90 100
LFRGDTVLLK GKKRRETVCI VLSDDTCSDE KVRMNRVVRN NLRVRLGDVI
110 120 130 140 150
SIQPCPDVKY GKRIHVLPID DTVEGITGNL FEVYLKPYFL EAYRPIRKGD
160 170 180 190 200
IFLVRGGMRA VEFKVVETDP SPYCIVAPDT VIHCEGEPIK REDEEESLNE
210 220 230 240 250
VGYDDIGGVR KQLAQIKEMV ELPLRHPALF KAIGVKPPRG ILLYGPPGTG
260 270 280 290 300
KTLIARAVAN ETGAFFFLIN GPEIMSKLAG ESESNLRKAF EEAEKNAPAI
310 320 330 340 350
IFIDELDAIA PKREKTHGEV ERRIVSQLLT LMDGLKQRAH VIVMAATNRP
360 370 380 390 400
NSIDPALRRF GRFDREVDIG IPDATGRLEI LQIHTKNMKL ADDVDLEQVA
410 420 430 440 450
NETHGHVGAD LAALCSEAAL QAIRKKMDLI DLEDETIDAE VMNSLAVTMD
460 470 480 490 500
DFRWALSQSN PSALRETVVE VPNITWEDIG GLDDVKRELQ ELVQYPVEHP
510 520 530 540 550
DKFLKFGMTP SKGVLFYGPP GCGKTLLAKA IANECQANFI SIKGPELLTM
560 570 580 590 600
WFGESEANVR EIFDKARQAA PCVLFFDELD SIAKARGGNV GDGGGAADRV
610 620 630 640 650
INQILTEMDG MSSKKNVFII GATNRPDIID PAILRPGRLD QLIYIPLPDE
660 670 680 690 700
KSRIAILKAN LRKSPISKDV DLDFLAKMTN GFSGADLTEI CQRACKLAIR
710 720 730 740 750
ESIENEIRRE RERQTNPSAM EVEEDDPVPE IRKDHFEEAM RFARRSVSDN
760 770 780 790 800
DIRKYEMFAQ TLQQSRGFGS FRFPSSNQGG SGPSQGSSGG GGGNVFNEDN

DDDLYG
Length:806
Mass (Da):89,424
Last modified:June 1, 2003 - v1
Checksum:iE126C61DFD7EE174
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti641 – 643QLI → HIM in BAC87740 (PubMed:12914916).Curated3
Sequence conflicti709R → L in BAC87740 (PubMed:12914916).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AB093594 mRNA Translation: BAC87740.1
AY576993 mRNA Translation: AAS92631.1
CR318632 Genomic DNA Translation: CAM13143.1
BC050488 mRNA Translation: AAH50488.1
BC067384 mRNA Translation: AAH67384.1
RefSeqiNP_958889.1, NM_201481.1
UniGeneiDr.75122

Genome annotation databases

EnsembliENSDART00000023779; ENSDARP00000012048; ENSDARG00000020008
GeneIDi327197
KEGGidre:327197

Similar proteinsi

Entry informationi

Entry nameiTERA_DANRE
AccessioniPrimary (citable) accession number: Q7ZU99
Secondary accession number(s): Q76KA4
Entry historyiIntegrated into UniProtKB/Swiss-Prot: September 1, 2009
Last sequence update: June 1, 2003
Last modified: April 25, 2018
This is version 135 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome