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Q7ZU99

- TERA_DANRE

UniProt

Q7ZU99 - TERA_DANRE

Protein

Transitional endoplasmic reticulum ATPase

Gene

vcp

Organism
Danio rerio (Zebrafish) (Brachydanio rerio)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 108 (01 Oct 2014)
      Sequence version 1 (01 Jun 2003)
      Previous versions | rss
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    Functioni

    Necessary for the fragmentation of Golgi stacks during mitosis and for their reassembly after mitosis. Involved in the formation of the nuclear envelope, and of the transitional endoplasmic reticulum (tER). The transfer of membranes from the endoplasmic reticulum to the Golgi apparatus occurs via 50-70 nm transition vesicles which derive from part-rough, part-smooth transitional elements of the endoplasmic reticulum (tER). Vesicle budding from the tER is an ATP-dependent process By similarity. Enhances cell cycle progression and inhibits apoptosis at low temperatures. Also involved in DNA damage response: recruited to double-strand breaks (DSBs) sites and promotes the recruitment of tp53bp1 at DNA damage sites.By similarity1 Publication

    Catalytic activityi

    ATP + H2O = ADP + phosphate.By similarity

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Binding sitei348 – 3481ATPBy similarity
    Binding sitei384 – 3841ATPBy similarity

    Regions

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Nucleotide bindingi247 – 2537ATPBy similarity

    GO - Molecular functioni

    1. ATP binding Source: UniProtKB-KW
    2. lipid binding Source: UniProtKB-KW
    3. nucleoside-triphosphatase activity Source: InterPro

    GO - Biological processi

    1. cell cycle Source: UniProtKB-KW
    2. cellular response to DNA damage stimulus Source: UniProtKB
    3. double-strand break repair Source: UniProtKB
    4. ER-associated ubiquitin-dependent protein catabolic process Source: UniProtKB
    5. protein N-linked glycosylation via asparagine Source: UniProtKB
    6. protein ubiquitination Source: UniProtKB
    7. regulation of protein ubiquitination involved in ubiquitin-dependent protein catabolic process Source: ZFIN
    8. translesion synthesis Source: UniProtKB
    9. transport Source: UniProtKB-KW

    Keywords - Molecular functioni

    Hydrolase

    Keywords - Biological processi

    Cell cycle, DNA damage, DNA repair, Transport

    Keywords - Ligandi

    ATP-binding, Lipid-binding, Nucleotide-binding

    Enzyme and pathway databases

    ReactomeiREACT_225331. HSF1 activation.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Transitional endoplasmic reticulum ATPaseBy similarity (EC:3.6.4.6)
    Short name:
    TER ATPaseBy similarity
    Alternative name(s):
    Protein CDC48Imported1 Publication
    Valosin-containing proteinImported1 Publication
    Short name:
    VCPBy similarity1 Publication
    Gene namesi
    Name:vcpImported
    Synonyms:cdc48
    ORF Names:si:ch211-113n10.2
    OrganismiDanio rerio (Zebrafish) (Brachydanio rerio)
    Taxonomic identifieri7955 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiActinopterygiiNeopterygiiTeleosteiOstariophysiCypriniformesCyprinidaeDanio
    ProteomesiUP000000437: Chromosome 5

    Organism-specific databases

    ZFINiZDB-GENE-030131-5408. vcp.

    Subcellular locationi

    Cytoplasmcytosol By similarity. Nucleus By similarity
    Note: Following DNA double-strand breaks, recruited to the sites of damage.By similarity

    GO - Cellular componenti

    1. cytosol Source: UniProtKB-SubCell
    2. nucleus Source: UniProtKB-SubCell
    3. site of double-strand break Source: UniProtKB

    Keywords - Cellular componenti

    Cytoplasm, Nucleus

    Pathology & Biotechi

    Mutagenesis

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Mutagenesisi805 – 8051Y → A: Inhibits cell-proliferation and enhances apoptosis at low temperatures. 1 Publication

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 806806Transitional endoplasmic reticulum ATPasePRO_0000382233Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Modified residuei3 – 31Phosphoserine1 Publication

    Keywords - PTMi

    Phosphoprotein

    Proteomic databases

    PRIDEiQ7ZU99.

    Expressioni

    Inductioni

    By cold.1 Publication

    Gene expression databases

    BgeeiQ7ZU99.

    Interactioni

    Subunit structurei

    Homohexamer.By similarity

    Protein-protein interaction databases

    IntActiQ7ZU99. 1 interaction.
    MINTiMINT-8283632.
    STRINGi7955.ENSDARP00000012048.

    Structurei

    3D structure databases

    ProteinModelPortaliQ7ZU99.
    SMRiQ7ZU99. Positions 18-763.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Sequence similaritiesi

    Belongs to the AAA ATPase family.Sequence Analysis

    Phylogenomic databases

    eggNOGiCOG0464.
    GeneTreeiENSGT00740000115575.
    HOGENOMiHOG000223224.
    InParanoidiQ76KA4.
    KOiK13525.
    OMAiEIFDKPC.
    OrthoDBiEOG7H4DSW.
    PhylomeDBiQ7ZU99.
    TreeFamiTF300542.

    Family and domain databases

    Gene3Di3.10.330.10. 1 hit.
    3.40.50.300. 2 hits.
    InterProiIPR003593. AAA+_ATPase.
    IPR005938. AAA_ATPase_CDC48.
    IPR009010. Asp_de-COase-like_dom.
    IPR003959. ATPase_AAA_core.
    IPR003960. ATPase_AAA_CS.
    IPR004201. Cdc48_dom2.
    IPR029067. CDC48_domain_2-like.
    IPR003338. CDC4_N-term_subdom.
    IPR027417. P-loop_NTPase.
    IPR015415. Vps4_C.
    [Graphical view]
    PfamiPF00004. AAA. 2 hits.
    PF02933. CDC48_2. 1 hit.
    PF02359. CDC48_N. 1 hit.
    PF09336. Vps4_C. 1 hit.
    [Graphical view]
    SMARTiSM00382. AAA. 2 hits.
    SM01072. CDC48_2. 1 hit.
    SM01073. CDC48_N. 1 hit.
    [Graphical view]
    SUPFAMiSSF50692. SSF50692. 1 hit.
    SSF52540. SSF52540. 2 hits.
    SSF54585. SSF54585. 1 hit.
    TIGRFAMsiTIGR01243. CDC48. 1 hit.
    PROSITEiPS00674. AAA. 2 hits.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Q7ZU99-1 [UniParc]FASTAAdd to Basket

    « Hide

    MASGGESKND DLSTAILKQK NRPNRLIVDE SINEDNSVVS LSQAKMDELQ    50
    LFRGDTVLLK GKKRRETVCI VLSDDTCSDE KVRMNRVVRN NLRVRLGDVI 100
    SIQPCPDVKY GKRIHVLPID DTVEGITGNL FEVYLKPYFL EAYRPIRKGD 150
    IFLVRGGMRA VEFKVVETDP SPYCIVAPDT VIHCEGEPIK REDEEESLNE 200
    VGYDDIGGVR KQLAQIKEMV ELPLRHPALF KAIGVKPPRG ILLYGPPGTG 250
    KTLIARAVAN ETGAFFFLIN GPEIMSKLAG ESESNLRKAF EEAEKNAPAI 300
    IFIDELDAIA PKREKTHGEV ERRIVSQLLT LMDGLKQRAH VIVMAATNRP 350
    NSIDPALRRF GRFDREVDIG IPDATGRLEI LQIHTKNMKL ADDVDLEQVA 400
    NETHGHVGAD LAALCSEAAL QAIRKKMDLI DLEDETIDAE VMNSLAVTMD 450
    DFRWALSQSN PSALRETVVE VPNITWEDIG GLDDVKRELQ ELVQYPVEHP 500
    DKFLKFGMTP SKGVLFYGPP GCGKTLLAKA IANECQANFI SIKGPELLTM 550
    WFGESEANVR EIFDKARQAA PCVLFFDELD SIAKARGGNV GDGGGAADRV 600
    INQILTEMDG MSSKKNVFII GATNRPDIID PAILRPGRLD QLIYIPLPDE 650
    KSRIAILKAN LRKSPISKDV DLDFLAKMTN GFSGADLTEI CQRACKLAIR 700
    ESIENEIRRE RERQTNPSAM EVEEDDPVPE IRKDHFEEAM RFARRSVSDN 750
    DIRKYEMFAQ TLQQSRGFGS FRFPSSNQGG SGPSQGSSGG GGGNVFNEDN 800
    DDDLYG 806
    Length:806
    Mass (Da):89,424
    Last modified:June 1, 2003 - v1
    Checksum:iE126C61DFD7EE174
    GO

    Experimental Info

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sequence conflicti641 – 6433QLI → HIM in BAC87740. (PubMed:12914916)Curated
    Sequence conflicti709 – 7091R → L in BAC87740. (PubMed:12914916)Curated

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AB093594 mRNA. Translation: BAC87740.1.
    AY576993 mRNA. Translation: AAS92631.1.
    CR318632 Genomic DNA. Translation: CAM13143.1.
    BC050488 mRNA. Translation: AAH50488.1.
    BC067384 mRNA. Translation: AAH67384.1.
    RefSeqiNP_958889.1. NM_201481.1.
    UniGeneiDr.75122.

    Genome annotation databases

    EnsembliENSDART00000023779; ENSDARP00000012048; ENSDARG00000020008.
    GeneIDi327197.
    KEGGidre:327197.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AB093594 mRNA. Translation: BAC87740.1 .
    AY576993 mRNA. Translation: AAS92631.1 .
    CR318632 Genomic DNA. Translation: CAM13143.1 .
    BC050488 mRNA. Translation: AAH50488.1 .
    BC067384 mRNA. Translation: AAH67384.1 .
    RefSeqi NP_958889.1. NM_201481.1.
    UniGenei Dr.75122.

    3D structure databases

    ProteinModelPortali Q7ZU99.
    SMRi Q7ZU99. Positions 18-763.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    IntActi Q7ZU99. 1 interaction.
    MINTi MINT-8283632.
    STRINGi 7955.ENSDARP00000012048.

    Proteomic databases

    PRIDEi Q7ZU99.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    Ensembli ENSDART00000023779 ; ENSDARP00000012048 ; ENSDARG00000020008 .
    GeneIDi 327197.
    KEGGi dre:327197.

    Organism-specific databases

    CTDi 7415.
    ZFINi ZDB-GENE-030131-5408. vcp.

    Phylogenomic databases

    eggNOGi COG0464.
    GeneTreei ENSGT00740000115575.
    HOGENOMi HOG000223224.
    InParanoidi Q76KA4.
    KOi K13525.
    OMAi EIFDKPC.
    OrthoDBi EOG7H4DSW.
    PhylomeDBi Q7ZU99.
    TreeFami TF300542.

    Enzyme and pathway databases

    Reactomei REACT_225331. HSF1 activation.

    Miscellaneous databases

    NextBioi 20809924.
    PROi Q7ZU99.

    Gene expression databases

    Bgeei Q7ZU99.

    Family and domain databases

    Gene3Di 3.10.330.10. 1 hit.
    3.40.50.300. 2 hits.
    InterProi IPR003593. AAA+_ATPase.
    IPR005938. AAA_ATPase_CDC48.
    IPR009010. Asp_de-COase-like_dom.
    IPR003959. ATPase_AAA_core.
    IPR003960. ATPase_AAA_CS.
    IPR004201. Cdc48_dom2.
    IPR029067. CDC48_domain_2-like.
    IPR003338. CDC4_N-term_subdom.
    IPR027417. P-loop_NTPase.
    IPR015415. Vps4_C.
    [Graphical view ]
    Pfami PF00004. AAA. 2 hits.
    PF02933. CDC48_2. 1 hit.
    PF02359. CDC48_N. 1 hit.
    PF09336. Vps4_C. 1 hit.
    [Graphical view ]
    SMARTi SM00382. AAA. 2 hits.
    SM01072. CDC48_2. 1 hit.
    SM01073. CDC48_N. 1 hit.
    [Graphical view ]
    SUPFAMi SSF50692. SSF50692. 1 hit.
    SSF52540. SSF52540. 2 hits.
    SSF54585. SSF54585. 1 hit.
    TIGRFAMsi TIGR01243. CDC48. 1 hit.
    PROSITEi PS00674. AAA. 2 hits.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Cold-inducible expression of the cell division cycle gene CDC48 and its promotion of cell proliferation during cold acclimation in zebrafish cells."
      Imamura S., Ojima N., Yamashita M.
      FEBS Lett. 549:14-20(2003) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA], FUNCTION, INDUCTION, MUTAGENESIS OF TYR-805.
      Tissue: Embryo1 Publication.
    2. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
      Tissue: Kidney marrow1 Publication.
    3. "The zebrafish reference genome sequence and its relationship to the human genome."
      Howe K., Clark M.D., Torroja C.F., Torrance J., Berthelot C., Muffato M., Collins J.E., Humphray S., McLaren K., Matthews L., McLaren S., Sealy I., Caccamo M., Churcher C., Scott C., Barrett J.C., Koch R., Rauch G.J.
      , White S., Chow W., Kilian B., Quintais L.T., Guerra-Assuncao J.A., Zhou Y., Gu Y., Yen J., Vogel J.H., Eyre T., Redmond S., Banerjee R., Chi J., Fu B., Langley E., Maguire S.F., Laird G.K., Lloyd D., Kenyon E., Donaldson S., Sehra H., Almeida-King J., Loveland J., Trevanion S., Jones M., Quail M., Willey D., Hunt A., Burton J., Sims S., McLay K., Plumb B., Davis J., Clee C., Oliver K., Clark R., Riddle C., Eliott D., Threadgold G., Harden G., Ware D., Mortimer B., Kerry G., Heath P., Phillimore B., Tracey A., Corby N., Dunn M., Johnson C., Wood J., Clark S., Pelan S., Griffiths G., Smith M., Glithero R., Howden P., Barker N., Stevens C., Harley J., Holt K., Panagiotidis G., Lovell J., Beasley H., Henderson C., Gordon D., Auger K., Wright D., Collins J., Raisen C., Dyer L., Leung K., Robertson L., Ambridge K., Leongamornlert D., McGuire S., Gilderthorp R., Griffiths C., Manthravadi D., Nichol S., Barker G., Whitehead S., Kay M., Brown J., Murnane C., Gray E., Humphries M., Sycamore N., Barker D., Saunders D., Wallis J., Babbage A., Hammond S., Mashreghi-Mohammadi M., Barr L., Martin S., Wray P., Ellington A., Matthews N., Ellwood M., Woodmansey R., Clark G., Cooper J., Tromans A., Grafham D., Skuce C., Pandian R., Andrews R., Harrison E., Kimberley A., Garnett J., Fosker N., Hall R., Garner P., Kelly D., Bird C., Palmer S., Gehring I., Berger A., Dooley C.M., Ersan-Urun Z., Eser C., Geiger H., Geisler M., Karotki L., Kirn A., Konantz J., Konantz M., Oberlander M., Rudolph-Geiger S., Teucke M., Osoegawa K., Zhu B., Rapp A., Widaa S., Langford C., Yang F., Carter N.P., Harrow J., Ning Z., Herrero J., Searle S.M., Enright A., Geisler R., Plasterk R.H., Lee C., Westerfield M., de Jong P.J., Zon L.I., Postlethwait J.H., Nusslein-Volhard C., Hubbard T.J., Roest Crollius H., Rogers J., Stemple D.L.
      Nature 496:498-503(2013) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: Tuebingen.
    4. NIH - Zebrafish Gene Collection (ZGC) project
      Submitted (MAR-2004) to the EMBL/GenBank/DDBJ databases
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
      Strain: ABImported.
      Tissue: KidneyImported.
    5. "Online automated in vivo zebrafish phosphoproteomics: from large-scale analysis down to a single embryo."
      Lemeer S., Pinkse M.W.H., Mohammed S., van Breukelen B., den Hertog J., Slijper M., Heck A.J.R.
      J. Proteome Res. 7:1555-1564(2008) [PubMed] [Europe PMC] [Abstract]
      Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-3, IDENTIFICATION BY MASS SPECTROMETRY.
      Tissue: Embryo1 Publication.

    Entry informationi

    Entry nameiTERA_DANRE
    AccessioniPrimary (citable) accession number: Q7ZU99
    Secondary accession number(s): Q76KA4
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: September 1, 2009
    Last sequence update: June 1, 2003
    Last modified: October 1, 2014
    This is version 108 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3