Q7ZSY6 (MT2_DANRE) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Metallothionein-2
Short name=MT-2

Gene names

Name:

mt2

Organism

Danio rerio (Zebrafish) (Brachydanio rerio)

Taxonomic identifier

7955 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Actinopterygii › Neopterygii › Teleostei › Ostariophysi › Cypriniformes › Cyprinidae › Danio

Protein attributes

Sequence length	60 AA.
Sequence status	Complete.
Protein existence	Evidence at transcript level

General annotation (Comments)

Function	Metallothioneins have a high content of cysteine residues that bind various heavy metals By similarity.
Developmental stage	Expressed both maternally and zygotically. Expressed ubiquitously during early embryogenesis, but becomes localized to the tail region during segmentation. During early larval development, expressed in discrete areas including the pronephric region, retina and cerebellum. Ref.2
Induction	By zinc ions. Ref.2
Domain	Class I metallothioneins contain 2 metal-binding domains: four divalent ions are chelated within cluster A of the alpha domain and are coordinated via cysteinyl thiolate bridges to 11 cysteine ligands. Cluster B, the corresponding region within the beta domain, can ligate three divalent ions to 9 cysteines.
Sequence similarities	Belongs to the metallothionein superfamily. Type 1 family.

Ontologies

Keywords
Ligand	Metal-binding Metal-thiolate cluster
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological process	response to cadmium ion Inferred from expression pattern. Source: ZFIN response to copper ion Inferred from direct assay. Source: ZFIN response to methylmercury Inferred from direct assay. Source: ZFIN response to zinc ion Inferred from direct assay. Source: ZFIN
Molecular function	metal ion binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 60	60	Metallothionein-2		PRO_0000197269
Regions
	Region	1 – 28	28	Beta
	Region	29 – 60	32	Alpha
Sites
	Metal binding	4	1	Divalent metal cation; cluster B
	Metal binding	6	1	Divalent metal cation; cluster B
	Metal binding	12	1	Divalent metal cation; cluster B
	Metal binding	14	1	Divalent metal cation; cluster B
	Metal binding	18	1	Divalent metal cation; cluster B
	Metal binding	20	1	Divalent metal cation; cluster B
	Metal binding	23	1	Divalent metal cation; cluster B
	Metal binding	25	1	Divalent metal cation; cluster B
	Metal binding	28	1	Divalent metal cation; cluster B
	Metal binding	32	1	Divalent metal cation; cluster A
	Metal binding	33	1	Divalent metal cation; cluster A
	Metal binding	35	1	Divalent metal cation; cluster A
	Metal binding	36	1	Divalent metal cation; cluster A
	Metal binding	40	1	Divalent metal cation; cluster A
	Metal binding	43	1	Divalent metal cation; cluster A
	Metal binding	47	1	Divalent metal cation; cluster A
	Metal binding	49	1	Divalent metal cation; cluster A
	Metal binding	54	1	Divalent metal cation; cluster A
	Metal binding	58	1	Divalent metal cation; cluster A
	Metal binding	59	1	Divalent metal cation; cluster A

Sequences

	Sequence		Length	Mass (Da)
	Q7ZSY6 [UniParc]. Last modified June 1, 2003. Version 1. Checksum: E5388E7C378BB700	FASTA	60	5,976
	10 20 30 40 50 60 MDPCECAKTG TCNCGATCKC TNCQCTTCKK SCCSCCPSGC SKCASGCVCK GNSCGSSCCQ

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Characterization of zebrafish metallothionein gene promoter in a zebrafish caudal fin cell-line, SJD. 1." Yan C.H.M., Chan K.M. Mar. Environ. Res. 54:335-339(2002) [PubMed: 12408584] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[2]	"Expression of metallothionein gene during embryonic and early larval development in zebrafish." Chen W.-Y., John J.A.C., Lin C.-H., Lin H.-F., Wu S.-C., Lin C.-H., Chang C.-Y. Aquat. Toxicol. 69:215-227(2004) [PubMed: 15276328] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA / MRNA], DEVELOPMENTAL STAGE, INDUCTION. Tissue: Brain.
[3]	NIH - Zebrafish Gene Collection (ZGC) project Submitted (APR-2003) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Strain: SJD.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AY305851 Genomic DNA. Translation: AAP73739.1. AY514790 mRNA. Translation: AAS00513.1. AY514791 Genomic DNA. Translation: AAS00514.1. BC049475 mRNA. Translation: AAH49475.1. BC051612 mRNA. Translation: AAH51612.1.
IPI	IPI00512698.
RefSeq	NP_001124525.2. NM_001131053.2.
UniGene	Dr.121290.
3D structure databases
ProteinModelPortal	Q7ZSY6.
SMR	Q7ZSY6. Positions 2-29, 31-60.
ModBase	Search...
Protein-protein interaction databases
STRING	Q7ZSY6.
Proteomic databases
PRIDE	Q7ZSY6.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
Ensembl	ENSDART00000061007; ENSDARP00000061006; ENSDARG00000041623.
GeneID	100174951.
KEGG	dre:100174951.
Organism-specific databases
CTD	17750.
ZFIN	ZDB-GENE-030131-4174. mt2.
Phylogenomic databases
GeneTree	ENSGT00390000010461.
HOVERGEN	HBG009063.
InParanoid	Q7ZSY6.
OMA	ETCPCPT.
Gene expression databases
ArrayExpress	Q7ZSY6.
Bgee	Q7ZSY6.
Family and domain databases
InterPro	IPR017854. Metalthion_dom. IPR023587. Metalthion_dom_vert. IPR003019. Metalthion_sfam_euk. IPR000006. Metalthion_vert. IPR018064. Metalthion_vert_metal_BS. [Graphical view]
Gene3D	G3DSA:4.10.10.10. Metallothionein_vert. 1 hit.
KO	K14739.
PANTHER	PTHR23299. Metallothionein_vert. 1 hit.
Pfam	PF00131. Metallothio. 1 hit. [Graphical view]
PRINTS	PR00860. MTVERTEBRATE.
SUPFAM	SSF57868. Metallothionein_sfam. 1 hit.
PROSITE	PS00203. METALLOTHIONEIN_VRT. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

MT2_DANRE

Accession

Primary (citable) accession number: Q7ZSY6

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 23, 2004
Last sequence update:	June 1, 2003
Last modified:	November 16, 2011
This is version 60 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program