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Q7ZSY6 (MT2_DANRE) Reviewed, UniProtKB/Swiss-Prot

Last modified April 16, 2014. Version 71. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Metallothionein-2

Short name=MT-2
Gene names
Name:mt2
OrganismDanio rerio (Zebrafish) (Brachydanio rerio) [Reference proteome]
Taxonomic identifier7955 [NCBI]
Taxonomic lineageEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiActinopterygiiNeopterygiiTeleosteiOstariophysiCypriniformesCyprinidaeDanio

Protein attributes

Sequence length60 AA.
Sequence statusComplete.
Protein existenceEvidence at transcript level

General annotation (Comments)

Function

Metallothioneins have a high content of cysteine residues that bind various heavy metals By similarity.

Developmental stage

Expressed both maternally and zygotically. Expressed ubiquitously during early embryogenesis, but becomes localized to the tail region during segmentation. During early larval development, expressed in discrete areas including the pronephric region, retina and cerebellum. Ref.2

Induction

By zinc ions. Ref.2

Domain

Class I metallothioneins contain 2 metal-binding domains: four divalent ions are chelated within cluster A of the alpha domain and are coordinated via cysteinyl thiolate bridges to 11 cysteine ligands. Cluster B, the corresponding region within the beta domain, can ligate three divalent ions to 9 cysteines.

Sequence similarities

Belongs to the metallothionein superfamily. Type 1 family.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 6060Metallothionein-2
PRO_0000197269

Regions

Region1 – 2828Beta
Region29 – 6032Alpha

Sites

Metal binding41Divalent metal cation; cluster B
Metal binding61Divalent metal cation; cluster B
Metal binding121Divalent metal cation; cluster B
Metal binding141Divalent metal cation; cluster B
Metal binding181Divalent metal cation; cluster B
Metal binding201Divalent metal cation; cluster B
Metal binding231Divalent metal cation; cluster B
Metal binding251Divalent metal cation; cluster B
Metal binding281Divalent metal cation; cluster B
Metal binding321Divalent metal cation; cluster A
Metal binding331Divalent metal cation; cluster A
Metal binding351Divalent metal cation; cluster A
Metal binding361Divalent metal cation; cluster A
Metal binding401Divalent metal cation; cluster A
Metal binding431Divalent metal cation; cluster A
Metal binding471Divalent metal cation; cluster A
Metal binding491Divalent metal cation; cluster A
Metal binding541Divalent metal cation; cluster A
Metal binding581Divalent metal cation; cluster A
Metal binding591Divalent metal cation; cluster A

Sequences

Sequence LengthMass (Da)Tools
Q7ZSY6 [UniParc].

Last modified June 1, 2003. Version 1.
Checksum: E5388E7C378BB700

FASTA605,976
        10         20         30         40         50         60 
MDPCECAKTG TCNCGATCKC TNCQCTTCKK SCCSCCPSGC SKCASGCVCK GNSCGSSCCQ 

« Hide

References

« Hide 'large scale' references
[1]"Characterization of zebrafish metallothionein gene promoter in a zebrafish caudal fin cell-line, SJD. 1."
Yan C.H.M., Chan K.M.
Mar. Environ. Res. 54:335-339(2002) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[2]"Expression of metallothionein gene during embryonic and early larval development in zebrafish."
Chen W.-Y., John J.A.C., Lin C.-H., Lin H.-F., Wu S.-C., Lin C.-H., Chang C.-Y.
Aquat. Toxicol. 69:215-227(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA / MRNA], DEVELOPMENTAL STAGE, INDUCTION.
Tissue: Brain.
[3]NIH - Zebrafish Gene Collection (ZGC) project
Submitted (APR-2003) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
Strain: SJD.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AY305851 Genomic DNA. Translation: AAP73739.1.
AY514790 mRNA. Translation: AAS00513.1.
AY514791 Genomic DNA. Translation: AAS00514.1.
BC049475 mRNA. Translation: AAH49475.1.
BC051612 mRNA. Translation: AAH51612.1.
RefSeqNP_001124525.2. NM_001131053.2.
UniGeneDr.121290.

3D structure databases

ProteinModelPortalQ7ZSY6.
SMRQ7ZSY6. Positions 2-29, 31-60.
ModBaseSearch...
MobiDBSearch...

Proteomic databases

PRIDEQ7ZSY6.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblENSDART00000061007; ENSDARP00000061006; ENSDARG00000041623.
GeneID100174951.
KEGGdre:100174951.

Organism-specific databases

CTD17750.
ZFINZDB-GENE-030131-4174. mt2.

Phylogenomic databases

GeneTreeENSGT00730000112385.
HOVERGENHBG009063.
InParanoidQ7ZSY6.
KOK14739.
OMACTNCNCK.
OrthoDBEOG7TBC98.

Gene expression databases

BgeeQ7ZSY6.

Family and domain databases

Gene3D4.10.10.10. 1 hit.
InterProIPR017854. Metalthion_dom.
IPR023587. Metalthion_dom_vert.
IPR003019. Metalthion_sfam_euk.
IPR000006. Metalthion_vert.
IPR018064. Metalthion_vert_metal_BS.
[Graphical view]
PANTHERPTHR23299. PTHR23299. 1 hit.
PfamPF00131. Metallothio. 1 hit.
[Graphical view]
PRINTSPR00860. MTVERTEBRATE.
SUPFAMSSF57868. SSF57868. 1 hit.
PROSITEPS00203. METALLOTHIONEIN_VRT. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

NextBio20795516.

Entry information

Entry nameMT2_DANRE
AccessionPrimary (citable) accession number: Q7ZSY6
Entry history
Integrated into UniProtKB/Swiss-Prot: November 23, 2004
Last sequence update: June 1, 2003
Last modified: April 16, 2014
This is version 71 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

Metallothioneins

Classification of metallothioneins and list of entries