UniProtKB - Q7ZAP3 (PHNX_SALTY)
(max 400 entries)x
Your basket is currently empty. i
Select item(s) and click on "Add to basket" to create your own collection here
(400 entries max)
Protein
Phosphonoacetaldehyde hydrolase
Gene
phnX
Organism
Salmonella typhimurium (strain LT2 / SGSC1412 / ATCC 700720)
Status
Functioni
Involved in phosphonate degradation.
Miscellaneous
Maps to a phosphate-starvation-inducible locus previously known as psiC.
Catalytic activityi
Phosphonoacetaldehyde + H2O = acetaldehyde + phosphate.
Cofactori
Mg2+By similarityNote: Binds 1 Mg2+ ion per subunit.By similarity
Kineticsi
- KM=40 µM for phosphonoacetaldehyde (at pH 7.0, 25 degrees Celsius)
Sites
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Active sitei | 10 | NucleophileBy similarity | 1 | |
Metal bindingi | 10 | MagnesiumBy similarity | 1 | |
Metal bindingi | 12 | Magnesium; via carbonyl oxygenBy similarity | 1 | |
Active sitei | 52 | Schiff-base intermediate with substrate | 1 | |
Metal bindingi | 186 | MagnesiumBy similarity | 1 |
GO - Molecular functioni
- metal ion binding Source: UniProtKB-KW
- phosphonoacetaldehyde hydrolase activity Source: UniProtKB-EC
GO - Biological processi
- organic phosphonate catabolic process Source: InterPro
Keywordsi
Molecular function | Hydrolase |
Ligand | Magnesium, Metal-binding, Schiff base |
Names & Taxonomyi
Protein namesi | Recommended name: Phosphonoacetaldehyde hydrolase (EC:3.11.1.1)Short name: Phosphonatase Alternative name(s): Phosphonoacetaldehyde phosphonohydrolase |
Gene namesi | Name:phnX Ordered Locus Names:STM0432 |
Organismi | Salmonella typhimurium (strain LT2 / SGSC1412 / ATCC 700720) |
Taxonomic identifieri | 99287 [NCBI] |
Taxonomic lineagei | Bacteria › Proteobacteria › Gammaproteobacteria › Enterobacterales › Enterobacteriaceae › Salmonella › |
Proteomesi |
|
Pathology & Biotechi
Mutagenesis
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Mutagenesisi | 52 | K → R: Complete loss of catalytic activity. 1 Publication | 1 |
PTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000284601 | 1 – 269 | Phosphonoacetaldehyde hydrolaseAdd BLAST | 269 |
Proteomic databases
PaxDbi | Q7ZAP3. |
Expressioni
Inductioni
Induced when inorganic phosphate is limiting; this is controlled by PhoB.1 Publication
Interactioni
Subunit structurei
Homodimer.By similarity
Protein-protein interaction databases
STRINGi | 99287.STM0432. |
Structurei
3D structure databases
ProteinModelPortali | Q7ZAP3. |
SMRi | Q7ZAP3. |
ModBasei | Search... |
MobiDBi | Search... |
Family & Domainsi
Sequence similaritiesi
Phylogenomic databases
eggNOGi | ENOG4108KAZ. Bacteria. COG0637. LUCA. |
HOGENOMi | HOG000217971. |
KOi | K05306. |
OMAi | KAVIFDW. |
Family and domain databases
CDDi | cd02586. HAD_PHN. 1 hit. |
Gene3Di | 1.10.150.240. 1 hit. 3.40.50.1000. 2 hits. |
HAMAPi | MF_01375. PhnX. 1 hit. |
InterProi | View protein in InterPro IPR036412. HAD-like_sf. IPR006439. HAD-SF_hydro_IA. IPR023214. HAD_sf. IPR023198. PGP-like_dom2. IPR006323. Phosphonoacetald_hydro. |
SUPFAMi | SSF56784. SSF56784. 1 hit. |
TIGRFAMsi | TIGR01509. HAD-SF-IA-v3. 1 hit. TIGR01422. phosphonatase. 1 hit. |
i Sequence
Sequence statusi: Complete.
Q7ZAP3-1 [UniParc]FASTAAdd to basket
10 20 30 40 50
MNRIHAVILD WAGTTVDFGS FAPTQIFVEA FRQAFDVEIT LAEARVPMGL
60 70 80 90 100
GKWQHIEALG KLPAVDARWQ AKFGRSMSAA DIDAIYAAFM PLQIAKVVDF
110 120 130 140 150
SSPIAGVIDT IAALRAEGIK IGSCSGYPRA VMERLVPAAA GHGYRPDHWV
160 170 180 190 200
ATDDLAAGGR PGPWMALQNV IALGIDAVAH CVKVDDAAPG ISEGLNAGMW
210 220 230 240 250
TVGLAVSGNE FGATWDAYQT MSKEDVAVRR EHAASKLYAA GAHYVVDSLA
260
DLPGVIAHIN ARLAQGERP
Sequence cautioni
The sequence AAB39641 differs from that shown. Reason: Erroneous initiation.Curated
The sequence AAL19386 differs from that shown. Reason: Erroneous initiation.Curated
Experimental Info
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Sequence conflicti | 242 – 265 | AHYVV…ARLAQ → RITWWIHWRIYL in AAB39641 (PubMed:9649311).CuratedAdd BLAST | 24 |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | U69493 Genomic DNA. Translation: AAB39641.1. Different initiation. AE006468 Genomic DNA. Translation: AAL19386.1. Different initiation. |
PIRi | T46946. |
RefSeqi | NP_459427.1. NC_003197.2. |
Genome annotation databases
EnsemblBacteriai | AAL19386; AAL19386; STM0432. |
GeneIDi | 1251951. |
KEGGi | stm:STM0432. |
PATRICi | fig|99287.12.peg.461. |
Similar proteinsi
Entry informationi
Entry namei | PHNX_SALTY | |
Accessioni | Q7ZAP3Primary (citable) accession number: Q7ZAP3 Secondary accession number(s): P96059 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | April 17, 2007 |
Last sequence update: | April 17, 2007 | |
Last modified: | March 28, 2018 | |
This is version 97 of the entry and version 2 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Prokaryotic Protein Annotation Program |