phnX

Functionⁱ

Involved in phosphonate degradation.

Miscellaneous

Maps to a phosphate-starvation-inducible locus previously known as psiC.

Catalytic activityⁱ

Phosphonoacetaldehyde + H₂O = acetaldehyde + phosphate.

Cofactorⁱ

Mg²⁺By similarityNote: Binds 1 Mg²⁺ ion per subunit.By similarity

Kineticsⁱ

K_M=40 µM for phosphonoacetaldehyde (at pH 7.0, 25 degrees Celsius)

Sites

Feature key	Position(s)	DescriptionActions	Length
Active siteⁱ	10	NucleophileBy similarity	1
Metal bindingⁱ	10	MagnesiumBy similarity	1
Metal bindingⁱ	12	Magnesium; via carbonyl oxygenBy similarity	1
Active siteⁱ	52	Schiff-base intermediate with substrate	1
Metal bindingⁱ	186	MagnesiumBy similarity	1

GO - Molecular functionⁱ

metal ion binding Source: UniProtKB-KW
phosphonoacetaldehyde hydrolase activity Source: UniProtKB-EC

Complete GO annotation...

GO - Biological processⁱ

organic phosphonate catabolic process Source: InterPro

Complete GO annotation...

Keywordsⁱ

Molecular function	Hydrolase
Ligand	Magnesium, Metal-binding, Schiff base

Names & Taxonomyⁱ

Protein namesⁱ	Recommended name: Phosphonoacetaldehyde hydrolase (EC:3.11.1.1) Short name: Phosphonatase Alternative name(s): Phosphonoacetaldehyde phosphonohydrolase
Gene namesⁱ	Name:phnX Ordered Locus Names:STM0432
Organismⁱ	Salmonella typhimurium (strain LT2 / SGSC1412 / ATCC 700720)
Taxonomic identifierⁱ	99287 [NCBI]
Taxonomic lineageⁱ	cellular organisms › Bacteria › Proteobacteria › Gammaproteobacteria › Enterobacterales › Enterobacteriaceae › Salmonella › Salmonella choleraesuis › Salmonella enterica I › Salmonella typhimurium
Proteomesⁱ	UP000001014 Componentⁱ: Chromosome

Pathology & Biotechⁱ

Mutagenesis

Feature key	Position(s)	DescriptionActions	Graphical view	Length
Mutagenesisⁱ	52	K → R: Complete loss of catalytic activity. 1 Publication		1

PTM / Processingⁱ

Molecule processing

Feature key	Position(s)	DescriptionActions	Graphical view	Length
ChainⁱPRO_0000284601	1 – 269	Phosphonoacetaldehyde hydrolaseAdd BLAST		269

Proteomic databases

PaxDbⁱ	Q7ZAP3.

PaxDbⁱ

Q7ZAP3.

Expressionⁱ

Inductionⁱ

Induced when inorganic phosphate is limiting; this is controlled by PhoB.1 Publication

Interactionⁱ

Subunit structureⁱ

Homodimer.By similarity

Protein-protein interaction databases

STRINGⁱ	99287.STM0432.

STRINGⁱ

99287.STM0432.

Structureⁱ

3D structure databases

ProteinModelPortalⁱ	Q7ZAP3.
SMRⁱ	Q7ZAP3.
ModBaseⁱ	Search...
MobiDBⁱ	Search...

Family & Domainsⁱ

Sequence similaritiesⁱ

Belongs to the HAD-like hydrolase superfamily. PhnX family.Curated

Phylogenomic databases

eggNOGⁱ	ENOG4108KAZ. Bacteria. COG0637. LUCA.
HOGENOMⁱ	HOG000217971.
KEGG Orthology (KO) More...KOⁱ	K05306.
OMAⁱ	KAVIFDW.

Family and domain databases

Conserved Domains Database More...CDDⁱ	cd02586. HAD_PHN. 1 hit.
Gene3Dⁱ	1.10.150.240. 1 hit. 3.40.50.1000. 1 hit.
HAMAPⁱ	MF_01375. PhnX. 1 hit.
InterProⁱ	View protein in InterPro IPR023214. HAD-like_dom. IPR006439. HAD-SF_hydro_IA. IPR023198. PGP_dom2. IPR006323. Phosphonoacetald_hydro.
SUPFAMⁱ	SSF56784. SSF56784. 1 hit.
TIGRFAMsⁱ	TIGR01509. HAD-SF-IA-v3. 1 hit. TIGR01422. phosphonatase. 1 hit.

Sequenceⁱ

Sequence statusⁱ: Complete.

Q7ZAP3-1 [UniParc]FASTA Add to basket

« Hide

        10         20         30         40         50
MNRIHAVILD WAGTTVDFGS FAPTQIFVEA FRQAFDVEIT LAEARVPMGL 
        60         70         80         90        100
GKWQHIEALG KLPAVDARWQ AKFGRSMSAA DIDAIYAAFM PLQIAKVVDF 
       110        120        130        140        150
SSPIAGVIDT IAALRAEGIK IGSCSGYPRA VMERLVPAAA GHGYRPDHWV 
       160        170        180        190        200
ATDDLAAGGR PGPWMALQNV IALGIDAVAH CVKVDDAAPG ISEGLNAGMW 
       210        220        230        240        250
TVGLAVSGNE FGATWDAYQT MSKEDVAVRR EHAASKLYAA GAHYVVDSLA 
       260 
DLPGVIAHIN ARLAQGERP

Length:269

Mass (Da):28,574

Last modified:April 17, 2007 - v2

Checksum:ⁱ0359B0E1124A3AF3

GO

Sequence cautionⁱ

The sequence AAB39641 differs from that shown. Reason: Erroneous initiation.Curated

The sequence AAL19386 differs from that shown. Reason: Erroneous initiation.Curated

Experimental Info

Feature key	Position(s)	DescriptionActions	Graphical view	Length
Sequence conflictⁱ	242 – 265	AHYVV…ARLAQ → RITWWIHWRIYL in AAB39641 (PubMed:9649311).CuratedAdd BLAST		24

Sequence databases

Select the link destinations: EMBLⁱ GenBankⁱ DDBJⁱ Links Updated	U69493 Genomic DNA. Translation: AAB39641.1. Different initiation. AE006468 Genomic DNA. Translation: AAL19386.1. Different initiation.
PIRⁱ	T46946.
NCBI Reference Sequences More...RefSeqⁱ	NP_459427.1. NC_003197.2. WP_000981902.1. NC_003197.2.

Genome annotation databases

EnsemblBacteriaⁱ	AAL19386; AAL19386; STM0432.
GeneIDⁱ	1251951.
KEGGⁱ	stm:STM0432.
PATRICⁱ	fig\|99287.12.peg.461.

Similar proteinsⁱ

Protein	Similar proteins	Organisms	Length	Cluster ID	Cluster name	Size
Q7ZAP3	A0A0D6HAJ3 A0A0W3RDH4 A0A0F7J617 A0A0U1IDZ9 E8XKL9 A0A0H3N8C4 A0A0F6AXQ8 UPI0009B13E7D	Salmonella typhimurium (strain LT2 / SGSC1412 / ATCC 700720) Salmonella typhimurium Salmonella choleraesuis Salmonella enterica I Salmonella enterica subsp. enterica serovar Typhimurium str. DT104 Salmonella typhimurium (strain 4/74) Salmonella typhimurium (strain SL1344) Salmonella typhimurium (strain 14028s / SGSC 2262)	269	UniRef100_Q7ZAP3	Cluster: Phosphonoacetaldehyde hydrolase	9

Protein	Similar proteins	Organisms	Length	Cluster ID	Cluster name	Size
Q7ZAP3	A0A0D6HAJ3 A0A0W3RDH4 A0A0F7J617 A0A0U1IDZ9 E8XKL9 A0A0H3N8C4 A0A0F6AXQ8 UPI0009B13E7D G5PZ08 G5RRH4 +166	Salmonella typhimurium (strain LT2 / SGSC1412 / ATCC 700720) Salmonella typhimurium Salmonella choleraesuis Salmonella enterica I Salmonella enterica subsp. enterica serovar Typhimurium str. DT104 Salmonella typhimurium (strain 4/74) Salmonella typhimurium (strain SL1344) Salmonella typhimurium (strain 14028s / SGSC 2262) Salmonella enterica subsp. enterica serovar Montevideo str. S5-403 Salmonella enterica subsp. enterica serovar Urbana str. R8-2977 And more	269	UniRef90_Q7ZAP3	Cluster: Phosphonoacetaldehyde hydrolase	177

Protein	Similar proteins	Organisms	Length	Cluster ID	Cluster name	Size
Q7ZAP3	Q9I433 UPI0006275639 A0A140SA31 UPI00053D99E1 UPI000449AE27 UPI0009FA6871 UPI0005DA2957 A0A086BV29 UPI0003BADEA6 UPI00087793CC +1859	Pseudomonas aeruginosa (strain ATCC 15692 / DSM 22644 / CIP 104116 / JCM 14847 / LMG 12228 / 1C / PRS 101 / PAO1) Pseudomonas aeruginosa Pseudomonas aeruginosa VRFPA01 Pseudomonas aeruginosa MH27 Pseudomonas aeruginosa (strain LESB58) Pseudomonas aeruginosa VRFPA03 Pseudomonas Pseudomonas aeruginosa (strain PA7) Pseudomonas knackmussii (strain DSM 6978 / LMG 23759 / B13) Pseudomonas sp. HMSC059F05 And more	275	UniRef50_Q9I433	Cluster: Phosphonoacetaldehyde hydrolase	1,870

Cross-referencesⁱ

Sequence databases

Select the link destinations: EMBLⁱ GenBankⁱ DDBJⁱ Links Updated	U69493 Genomic DNA. Translation: AAB39641.1. Different initiation. AE006468 Genomic DNA. Translation: AAL19386.1. Different initiation.
PIRⁱ	T46946.
NCBI Reference Sequences More...RefSeqⁱ	NP_459427.1. NC_003197.2. WP_000981902.1. NC_003197.2.

3D structure databases

ProteinModelPortalⁱ	Q7ZAP3.
SMRⁱ	Q7ZAP3.
ModBaseⁱ	Search...
MobiDBⁱ	Search...

Protein-protein interaction databases

STRINGⁱ	99287.STM0432.

STRINGⁱ

99287.STM0432.

Proteomic databases

PaxDbⁱ	Q7ZAP3.

PaxDbⁱ

Q7ZAP3.

Protocols and materials databases

Structural Biology Knowledgebase	Search...

Structural Biology Knowledgebase

Search...

Genome annotation databases

EnsemblBacteriaⁱ	AAL19386; AAL19386; STM0432.
GeneIDⁱ	1251951.
KEGGⁱ	stm:STM0432.
PATRICⁱ	fig\|99287.12.peg.461.

Phylogenomic databases

eggNOGⁱ	ENOG4108KAZ. Bacteria. COG0637. LUCA.
HOGENOMⁱ	HOG000217971.
KEGG Orthology (KO) More...KOⁱ	K05306.
OMAⁱ	KAVIFDW.

Family and domain databases

Conserved Domains Database More...CDDⁱ	cd02586. HAD_PHN. 1 hit.
Gene3Dⁱ	1.10.150.240. 1 hit. 3.40.50.1000. 1 hit.
HAMAPⁱ	MF_01375. PhnX. 1 hit.
InterProⁱ	View protein in InterPro IPR023214. HAD-like_dom. IPR006439. HAD-SF_hydro_IA. IPR023198. PGP_dom2. IPR006323. Phosphonoacetald_hydro.
SUPFAMⁱ	SSF56784. SSF56784. 1 hit.
TIGRFAMsⁱ	TIGR01509. HAD-SF-IA-v3. 1 hit. TIGR01422. phosphonatase. 1 hit.
ProtoNetⁱ	Search...

Entry informationⁱ

Entry nameⁱ	PHNX_SALTY
Accessionⁱ	Q7ZAP3Primary (citable) accession number: Q7ZAP3 Secondary accession number(s): P96059
Entry historyⁱ	Integrated into UniProtKB/Swiss-Prot:	April 17, 2007
	Last sequence update:	April 17, 2007
	Last modified:	September 27, 2017
	This is version 93 of the entry and version 2 of the sequence. See complete history.
Entry statusⁱ	Reviewed (UniProtKB/Swiss-Prot)
Annotation program	Prokaryotic Protein Annotation Program

UniProtKB

UniParc

Help

UniRef

Proteomes

Annotation systems

Supporting data

UniProtKB - Q7ZAP3 (PHNX_SALTY)

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Phosphonoacetaldehyde hydrolase

Select a section on the left to see content.

<p>This section provides any useful information about the protein, mostly biological knowledge.<p><a href='/help/function_section' target='_top'>More...</a></p>Functioni

Miscellaneous

<p>This subsection of the ‘Function’ section describes biophysical and chemical properties, such as maximal absorption, kinetic parameters, pH dependence, redox potentials and temperature dependence.<p><a href='/help/biophysicochemical_properties' target='_top'>More...</a></p>Kineticsi

Sites

<p>The <a href="http://www.geneontology.org/">Gene Ontology (GO)</a> project provides a set of hierarchical controlled vocabulary split into 3 categories:<p><a href='/help/gene_ontology' target='_top'>More...</a></p>GO - Molecular functioni

<p>The <a href="http://www.geneontology.org/">Gene Ontology (GO)</a> project provides a set of hierarchical controlled vocabulary split into 3 categories:<p><a href='/help/gene_ontology' target='_top'>More...</a></p>GO - Biological processi

<p>This section provides information about the protein and gene name(s) and synonym(s) and about the organism that is the source of the protein sequence.<p><a href='/help/names_and_taxonomy_section' target='_top'>More...</a></p>Names & Taxonomyi

<p>This section provides information on the disease(s) and phenotype(s) associated with a protein.<p><a href='/help/pathology_and_biotech_section' target='_top'>More...</a></p>Pathology & Biotechi

Mutagenesis

<p>This section describes post-translational modifications (PTMs) and/or processing events.<p><a href='/help/ptm_processing_section' target='_top'>More...</a></p>PTM / Processingi

Molecule processing

Proteomic databases

<p>This section provides information on the expression of a gene at the mRNA or protein level in cells or in tissues of multicellular organisms.<p><a href='/help/expression_section' target='_top'>More...</a></p>Expressioni

<p>This section provides information on the quaternary structure of a protein and on interaction(s) with other proteins or protein complexes.<p><a href='/help/interaction_section' target='_top'>More...</a></p>Interactioni

Protein-protein interaction databases

<p>This section provides information on the tertiary and secondary structure of a protein.<p><a href='/help/structure_section' target='_top'>More...</a></p>Structurei

3D structure databases

<p>This section provides information on sequence similarities with other proteins and the domain(s) present in a protein.<p><a href='/help/family_and_domains_section' target='_top'>More...</a></p>Family & Domainsi

<p>This subsection of the ‘Family and domains’ section provides information about the sequence similarity with other proteins.<p><a href='/help/sequence_similarities' target='_top'>More...</a></p>Sequence similaritiesi

Phylogenomic databases

Family and domain databases

<p>This section displays by default the canonical protein sequence and upon request all isoforms described in the entry. It also includes information pertinent to the sequence(s), including length and molecular weight.<p><a href='/help/sequences_section' target='_top'>More...</a></p>Sequencei

<p>This subsection of the ‘Sequence’ section reports difference(s) between the protein sequence shown in the UniProtKB entry and other available protein sequences derived from the same gene.<p><a href='/help/sequence_caution' target='_top'>More...</a></p>Sequence cautioni

Experimental Info

Sequence databases

Genome annotation databases

<p>This section is used to point to information related to entries and found in data collections other than UniProtKB.<p><a href='/help/cross_references_section' target='_top'>More...</a></p>Cross-referencesi

Sequence databases

3D structure databases

Protein-protein interaction databases

Proteomic databases

Protocols and materials databases

Genome annotation databases

Phylogenomic databases

Family and domain databases

<p>This section provides general information on the entry.<p><a href='/help/entry_information_section' target='_top'>More...</a></p>Entry informationi

<p>This section contains any relevant information that doesn’t fit in any other defined sections<p><a href='/help/miscellaneous_section' target='_top'>More...</a></p>Miscellaneousi

Documents

Functionⁱ

Kineticsⁱ

GO - Molecular functionⁱ

GO - Biological processⁱ

Names & Taxonomyⁱ

Pathology & Biotechⁱ

PTM / Processingⁱ

Expressionⁱ

Interactionⁱ

Structureⁱ

Family & Domainsⁱ

Sequence similaritiesⁱ

Sequenceⁱ

Sequence cautionⁱ

Cross-referencesⁱ

Entry informationⁱ

Miscellaneousⁱ