Reviewed, UniProtKB/Swiss-Prot Q7ZA45 (GPD_KLUTH)

Names and origin

Protein names

Recommended name:
    Glycerol-3-phosphate dehydrogenase [NAD+]
    EC=1.1.1.8

Gene names

Name:

GPD

Organism

Kluyveromyces thermotolerans (Yeast)

Taxonomic identifier

4916 [NCBI]

Taxonomic lineage

Eukaryota › Fungi › Dikarya › Ascomycota › Saccharomycotina › Saccharomycetes › Saccharomycetales › Saccharomycetaceae › Kluyveromyces

Protein attributes

Sequence length	411 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Catalytic activity	sn-glycerol 3-phosphate + NAD(+) = glycerone phosphate + NADH.
Sequence similarities	Belongs to the NAD-dependent glycerol-3-phosphate dehydrogenase family.

Ontologies

Keywords
Ligand	NAD
Molecular function	Oxidoreductase
Gene Ontology (GO)
Biological process	carbohydrate metabolic process Inferred from electronic annotation. Source: InterPro glycerol-3-phosphate catabolic process Inferred from electronic annotation. Source: InterPro oxidation reduction Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	glycerol-3-phosphate dehydrogenase complex Inferred from electronic annotation. Source: InterPro
Molecular function	NAD binding Inferred from electronic annotation. Source: InterPro glycerol-3-phosphate dehydrogenase (NAD+) activity Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 411	411	Glycerol-3-phosphate dehydrogenase [NAD+]		PRO_0000138090
Regions
	Nucleotide binding	71 – 76	6	NAD By similarity
	Region	340 – 341	2	Substrate binding By similarity
Sites
	Active site	275	1	Proton acceptor By similarity
	Binding site	159	1	NAD By similarity
	Binding site	182	1	NAD; via amide nitrogen By similarity
	Binding site	182	1	Substrate By similarity
	Binding site	215	1	NAD; via amide nitrogen By similarity
	Binding site	340	1	NAD By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q7ZA45-1 [UniParc]. Last modified October 1, 2003. Version 1. Checksum: A155FF3F16521A86	FASTA	411	45,332
	10 20 30 40 50 60 MFSISRITRT SSFTTQFRAL YRFKHSARKL QSIPFSIYKK MSAADRLNQT HDILSESVQA 70 80 90 100 110 120 VENPFKVTVI GSGNWGTTIS KVVAENAALR PHLFVKRVDM WVFEETVDGQ KLTEIINTKH 130 140 150 160 170 180 QNVKYLPNID LPENLVANPD LVSAVKDADI LVFNIPHQFL PRIVSQLQGN IKKDARAISC 190 200 210 220 230 240 LKGFDVSKDG VKLLSTYVTE KLGITCGALS GANLAPEVAK ENWSETTVAY ELPKDFKGEG 250 260 270 280 290 300 KDVDHAVLKA LFHRPYFHVN VIDDVAGISV AGALKNVVAL GCGFVEGLGW GNNASAAIQR 310 320 330 340 350 360 VGLGEIIKFG QMFFPDSRVE TYYQESAGVA DLITTCSGGR NVRVATHMAK TGKSAEECEK 370 380 390 400 410 ELLNGQSAQV FTHVRRSTSG WPSAVRPMNS FCSRPFTRLS TRTLLWTLCQ T

References

[1]	"Yeast orthologues associated with glycerol transport and metabolism." Neves L., Oliveira R., Lucas C. FEMS Yeast Res. 5:51-62(2004) [PubMed: 15381122] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: CBS 137.

Cross-references

Sequence databases
EMBLGenBankDDBJ	AY289713 Genomic DNA. Translation: AAP44104.1.
3D structure databases
HSSP	HSSP built from PDB template 1EVY based on UniProtKB P90551.
ModBase	Search...
Family and domain databases
InterPro	IPR013328. DHase_multihelical. IPR016040. NAD(P)-bd. IPR017751. NAD-dep_Gly3P_DH_euk. IPR006168. NAD-dep_Gly3P_DHase. IPR011128. NAD-dep_Gly3P_DHase_N. IPR006109. NAD_Gly3P_DHase_C. [Graphical view]
Gene3D	G3DSA:3.40.50.720. NAD(P)-bd. 1 hit. G3DSA:1.10.1040.10. Opine_DH. 1 hit.
PANTHER	PTHR11728. NAD_Gly3P_DH. 1 hit.
Pfam	PF07479. NAD_Gly3P_dh_C. 1 hit. PF01210. NAD_Gly3P_dh_N. 1 hit. [Graphical view]
PIRSF	PIRSF000114. Glycerol-3-P_dh. 1 hit.
PRINTS	PR00077. GPDHDRGNASE.
ProDom	PD001278. NAD_Gly3P_C. 1 hit. [Graphical view] [Entries sharing at least one domain]
PROSITE	PS00957. NAD_G3PDH. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

GPD_KLUTH

Accession

Primary (citable) accession number: Q7ZA45

Entry history

Integrated into UniProtKB/Swiss-Prot:	January 10, 2006
Last sequence update:	October 1, 2003
Last modified:	November 25, 2008
This is version 28 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

FPAP (Fungal Proteome Annotation Project)