Reviewed, UniProtKB/Swiss-Prot Q7ZA43 (GPD_PICAN)

Names and origin

Protein names

Recommended name:
    Glycerol-3-phosphate dehydrogenase [NAD+]
    EC=1.1.1.8

Gene names

Name:

GPD

Organism

Pichia angusta (Yeast) (Hansenula polymorpha)

Taxonomic identifier

4905 [NCBI]

Taxonomic lineage

Eukaryota › Fungi › Dikarya › Ascomycota › Saccharomycotina › Saccharomycetes › Saccharomycetales › Saccharomycetaceae › Pichia

Protein attributes

Sequence length	381 AA.
Sequence status	Complete.
Protein existence	Inferred from homology.

General annotation (Comments)

Catalytic activity	sn-glycerol 3-phosphate + NAD+ = glycerone phosphate + NADH.
Sequence similarities	Belongs to the NAD-dependent glycerol-3-phosphate dehydrogenase family.

Ontologies

Keywords
Ligand	NAD
Molecular function	Oxidoreductase
Gene Ontology (GO)
Biological process	glycerol-3-phosphate catabolic process Inferred from electronic annotation. Source: InterPro oxidation reduction Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	glycerol-3-phosphate dehydrogenase complex Inferred from electronic annotation. Source: InterPro
Molecular function	NAD or NADH binding Inferred from electronic annotation. Source: InterPro glycerol-3-phosphate dehydrogenase (NAD+) activity Inferred from electronic annotation. Source: EC protein homodimerization activity Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 381	381	Glycerol-3-phosphate dehydrogenase [NAD+]		PRO_0000138092
Regions
	Nucleotide binding	34 – 39	6	NAD By similarity
	Region	303 – 304	2	Substrate binding By similarity
Sites
	Active site	238	1	Proton acceptor By similarity
	Binding site	122	1	NAD By similarity
	Binding site	145	1	NAD; via amide nitrogen By similarity
	Binding site	145	1	Substrate By similarity
	Binding site	178	1	NAD; via amide nitrogen By similarity
	Binding site	303	1	NAD By similarity
	Binding site	332	1	NAD By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q7ZA43-1 [UniParc]. Last modified October 1, 2003. Version 1. Checksum: D1470A0A7B12D7C6	FASTA	381	42,205
	10 20 30 40 50 60 MTAMDRLDHV SNQLAAKRQK KNPEGKPFRI TVVGSGNWGS TIAKVVAENA KELPEEFHQI 70 80 90 100 110 120 VKMWVFEEEV DGRKLTEIIN TDHENVKYLP DVKLPDNIVA IPDIVDACAD ADIIIFNIPH 130 140 150 160 170 180 QFLPKILAQL KGKVNPKARA ISCLKGLEVT KDGCKLLSNY ITEELGIYCG ALSGANLAPE 190 200 210 220 230 240 VARQKWSETT VAYRIPQDFR GEGKDVDQSV IRNLFHRPYF HVRVIDDVAG VSLSGALKNV 250 260 270 280 290 300 IAMAAGFVEG LGWGDNAKSA VMRIGLVEMI KFAHMFFEDC QSTTFTHESA GVADIITTCA 310 320 330 340 350 360 GGRNVRVGRY MAEHKVSGFE AEKVLLNGQS CQGLHTTREV YELLAAKNVI DEFPLFKATY 370 380 QIIYEGLPME KLPELLEASE D

References

[1]	"Yeast orthologues associated with glycerol transport and metabolism." Neves L., Oliveira R., Lucas C. FEMS Yeast Res. 5:51-62(2004) [PubMed: 15381122] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: IGc4129.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AY289714 Genomic DNA. Translation: AAP44106.1.
3D structure databases
SMR	Q7ZA43. Positions 28-376.
ModBase	Search...
Enzyme and pathway databases
BRENDA	1.1.1.8. 276392.
Family and domain databases
InterPro	IPR008927. 6-PGluconate_DH_C-like. IPR013328. DH_multihelical. IPR016040. NAD(P)-bd_dom. IPR006168. NAD-dep_Gly3P_DH. IPR017751. NAD-dep_Gly3P_DH_euk. IPR011128. NAD-dep_Gly3P_DH_N. IPR006109. NAD_Gly3P_DH_C. [Graphical view]
Gene3D	G3DSA:3.40.50.720. NAD(P)-bd. 1 hit. G3DSA:1.10.1040.10. Opine_DH. 1 hit.
PANTHER	PTHR11728. NAD_Gly3P_DH. 1 hit.
Pfam	PF07479. NAD_Gly3P_dh_C. 1 hit. PF01210. NAD_Gly3P_dh_N. 1 hit. [Graphical view]
PIRSF	PIRSF000114. Glycerol-3-P_dh. 1 hit.
PRINTS	PR00077. GPDHDRGNASE.
TIGRFAMs	TIGR03376. glycerol3P_DH. 1 hit.
PROSITE	PS00957. NAD_G3PDH. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

GPD_PICAN

Accession

Primary (citable) accession number: Q7ZA43

Entry history

Integrated into UniProtKB/Swiss-Prot:	January 10, 2006
Last sequence update:	October 1, 2003
Last modified:	February 9, 2010
This is version 36 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

FPAP (Fungal Proteome Annotation Project)