Q7Z9M7 (GUN7_HYPJQ) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Endoglucanase-7
EC=3.2.1.4

Alternative name(s):
Cellulase-61B
Short name=Cel61B
Endo-1,4-beta-glucanase VII
Short name=EGVII
Endoglucanase VII
Endoglucanase-61B

Gene names

Name:	cel61b
Synonyms:	egl7
ORF Names:	TRIREDRAFT_120961

Organism

Hypocrea jecorina (strain QM6a) (Trichoderma reesei) [Complete proteome]

Taxonomic identifier

431241 [NCBI]

Taxonomic lineage

Eukaryota › Fungi › Dikarya › Ascomycota › Pezizomycotina › Sordariomycetes › Hypocreomycetidae › Hypocreales › Hypocreaceae › Hypocrea

Protein attributes

Sequence length	249 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Has low levels of endoglucanase activity. May be involved in the degradation of cellulose or lignocellulose, chitin, or other polysaccharides.
Catalytic activity	Endohydrolysis of (1->4)-beta-D-glucosidic linkages in cellulose, lichenin and cereal beta-D-glucans. Ref.3
Cofactor	Calcium Probable.
Subunit structure	Monomer. Ref.3
Subcellular location	Secreted Ref.3.
Induction	By cellulose, lactose and sophorose. Ref.1
Sequence similarities	Belongs to the glycosyl hydrolase 61 family.

Ontologies

Keywords
Biological process	Carbohydrate metabolism Cellulose degradation Polysaccharide degradation
Cellular component	Secreted
Domain	Signal
Ligand	Calcium Metal-binding
Molecular function	Glycosidase Hydrolase
PTM	Disulfide bond Glycoprotein
Technical term	3D-structure Complete proteome
Gene Ontology (GO)
Biological process	cellulose catabolic process Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	extracellular region Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	cellulase activity Inferred from electronic annotation. Source: EC metal ion binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Signal peptide

1 – 19

19

Potential

Chain

20 – 249

230

Endoglucanase-7

PRO_0000364090

Sites

Metal binding

20

1

Divalent metal cation

Metal binding

108

1

Divalent metal cation

Metal binding

195

1

Divalent metal cation

Amino acid modifications

Glycosylation

25

1

N-linked (GlcNAc...)

Disulfide bond

78 ↔ 198

Disulfide bond

120 ↔ 124

Experimental info

Sequence conflict

118

1

A → V in AAP57753. Ref.1

Secondary structure

1

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249

Helix Strand Turn

Details...

Beta strand

25 – 28

4

Helix

38 – 46

9

Helix

68 – 70

3

Helix

75 – 78

4

Beta strand

89 – 92

4

Beta strand

96 – 100

5

Beta strand

113 – 119

7

Helix

124 – 126

3

Helix

129 – 131

3

Beta strand

135 – 140

6

Turn

144 – 147

4

Helix

150 – 156

7

Beta strand

160 – 164

5

Beta strand

174 – 182

9

Beta strand

193 – 204

12

Helix

217 – 219

3

Beta strand

232 – 234

3

Sequences

	Sequence		Length	Mass (Da)
	Q7Z9M7 [UniParc]. Last modified November 16, 2011. Version 2. Checksum: 1BD57A744D48F0EF	FASTA	249	26,800
	10 20 30 40 50 60 MKSCAILAAL GCLAGSVLGH GQVQNFTING QYNQGFILDY YYQKQNTGHF PNVAGWYAED 70 80 90 100 110 120 LDLGFISPDQ YTTPDIVCHK NAAPGAISAT AAAGSNIVFQ WGPGVWPHPY GPIVTYVAEC 130 140 150 160 170 180 SGSCTTVNKN NLRWVKIQEA GINYNTQVWA QQDLINQGNK WTVKIPSSLR PGNYVFRHEL 190 200 210 220 230 240 LAAHGASSAN GMQNYPQCVN IAVTGSGTKA LPAGTPATQL YKPTDPGILF NPYTTITSYT IPGPALWQG

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Transcriptional regulation of biomass-degrading enzymes in the filamentous fungus Trichoderma reesei." Foreman P.K., Brown D., Dankmeyer L., Dean R., Diener S., Dunn-Coleman N.S., Goedegebuur F., Houfek T.D., England G.J., Kelley A.S., Meerman H.J., Mitchell T., Mitchinson C., Olivares H.A., Teunissen P.J.M., Yao J., Ward M. J. Biol. Chem. 278:31988-31997(2003) [PubMed: 12788920] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA], INDUCTION. Strain: QM6a.
[2]	"Genome sequencing and analysis of the biomass-degrading fungus Trichoderma reesei (syn. Hypocrea jecorina)." Martinez D., Berka R.M., Henrissat B., Saloheimo M., Arvas M., Baker S.E., Chapman J., Chertkov O., Coutinho P.M., Cullen D., Danchin E.G., Grigoriev I.V., Harris P., Jackson M., Kubicek C.P., Han C.S., Ho I., Larrondo L.F. , de Leon A.L., Magnuson J.K., Merino S., Misra M., Nelson B., Putnam N., Robbertse B., Salamov A.A., Schmoll M., Terry A., Thayer N., Westerholm-Parvinen A., Schoch C.L., Yao J., Barabote R., Nelson M.A., Detter C., Bruce D., Kuske C.R., Xie G., Richardson P., Rokhsar D.S., Lucas S.M., Rubin E.M., Dunn-Coleman N., Ward M., Brettin T.S. Nat. Biotechnol. 26:553-560(2008) [PubMed: 18454138] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: QM6a.
[3]	"The first structure of a glycoside hydrolase family 61 member, Cel61B from Hypocrea jecorina, at 1.6 A resolution." Karkehabadi S., Hansson H., Kim S., Piens K., Mitchinson C., Sandgren M. J. Mol. Biol. 383:144-154(2008) [PubMed: 18723026] [Abstract] Cited for: X-RAY CRYSTALLOGRAPHY (1.6 ANGSTROMS) OF 20-249, SUBUNIT, CATALYTIC ACTIVITY, SUBCELLULAR LOCATION. Strain: QM6a.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ

AY281372 mRNA. Translation: AAP57753.1.
GL985060 Genomic DNA. Translation: EGR50392.1.

3D structure databases

PDBe
RCSB PDB
PDBj

Entry	Method	Resolution (Å)	Chain	Positions	PDBsum
2VTC	X-ray	1.60	A/B	1-249	[»]

ModBase

Search...

Protein family/group databases

CAZy

GH61. Glycoside Hydrolase Family 61.

Protocols and materials databases

StructuralBiologyKnowledgebase

Search...

Enzyme and pathway databases

BioCyc

MetaCyc:MONOMER-16506.

Family and domain databases

InterPro

IPR003961. Fibronectin_type3.
IPR005103. Glyco_hydro_61.
[Graphical view]

Pfam

PF03443. Glyco_hydro_61. 1 hit.
[Graphical view]

SMART

SM00060. FN3. 1 hit.
[Graphical view]

ProtoNet

Search...

Entry information

Entry name

GUN7_HYPJQ

Accession

Primary (citable) accession number: Q7Z9M7
Secondary accession number(s): G0RER9

Entry history

Integrated into UniProtKB/Swiss-Prot:	February 10, 2009
Last sequence update:	November 16, 2011
Last modified:	January 25, 2012
This is version 31 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Fungal Protein Annotation Program