Reviewed, UniProtKB/Swiss-Prot Q7Z9M7 (GUN7_TRIRE)

Names and origin

Protein names

Recommended name:
    Endoglucanase-7
    EC=3.2.1.4
Alternative name(s):
    Endoglucanase VII
    Endo-1,4-beta-glucanase VII
      Short name=EGVII
    Endoglucanase-61B
    Cellulase-61B
      Short name=Cel61B

Gene names

Name:	cel61b
Synonyms:	egl7

Organism

Trichoderma reesei (Hypocrea jecorina)

Taxonomic identifier

51453 [NCBI]

Taxonomic lineage

Eukaryota › Fungi › Dikarya › Ascomycota › Pezizomycotina › Sordariomycetes › Hypocreomycetidae › Hypocreales › Hypocreaceae › Hypocrea

Protein attributes

Sequence length	249 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at protein level.

General annotation (Comments)

Function	Has low levels of endoglucanase activity. May be involved in the degradation of cellulose or lignocellulose, chitin, or other polysaccharides.
Catalytic activity	Endohydrolysis of (1->4)-beta-D-glucosidic linkages in cellulose, lichenin and cereal beta-D-glucans. Ref.2
Cofactor	Calcium Probable.
Subunit structure	Monomer. Ref.2
Subcellular location	Secreted. Ref.2
Induction	By cellulose, lactose and sophorose. Ref.1
Sequence similarities	Belongs to the glycosyl hydrolase 61 family.

Ontologies

Keywords
Biological process	Carbohydrate metabolism Cellulose degradation Polysaccharide degradation
Cellular component	Secreted
Domain	Signal
Ligand	Calcium Metal-binding
Molecular function	Glycosidase Hydrolase
PTM	Disulfide bond Glycoprotein
Technical term	3D-structure
Gene Ontology (GO)
Biological process	cellulose catabolic process Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	extracellular region Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	calcium ion binding Inferred from electronic annotation. Source: UniProtKB-KW cellulase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Signal peptide	1 – 19	19	Potential
	Chain	20 – 249	230	Endoglucanase-7		PRO_0000364090
Sites
	Metal binding	20	1	Divalent metal cation
	Metal binding	108	1	Divalent metal cation
	Metal binding	195	1	Divalent metal cation
Amino acid modifications
	Glycosylation	25	1	N-linked (GlcNAc...)
	Disulfide bond	78 ↔ 198
	Disulfide bond	120 ↔ 124

Sequences

	Sequence		Length	Mass (Da)
	Q7Z9M7-1 [UniParc]. Last modified October 1, 2003. Version 1. Checksum: 1BC7F179D657E0EF	FASTA	249	26,828
	10 20 30 40 50 60 MKSCAILAAL GCLAGSVLGH GQVQNFTING QYNQGFILDY YYQKQNTGHF PNVAGWYAED 70 80 90 100 110 120 LDLGFISPDQ YTTPDIVCHK NAAPGAISAT AAAGSNIVFQ WGPGVWPHPY GPIVTYVVEC 130 140 150 160 170 180 SGSCTTVNKN NLRWVKIQEA GINYNTQVWA QQDLINQGNK WTVKIPSSLR PGNYVFRHEL 190 200 210 220 230 240 LAAHGASSAN GMQNYPQCVN IAVTGSGTKA LPAGTPATQL YKPTDPGILF NPYTTITSYT IPGPALWQG

References

[1]

"Transcriptional regulation of biomass-degrading enzymes in the filamentous fungus Trichoderma reesei." Foreman P.K., Brown D., Dankmeyer L., Dean R., Diener S., Dunn-Coleman N.S., Goedegebuur F., Houfek T.D., England G.J., Kelley A.S., Meerman H.J., Mitchell T., Mitchinson C., Olivares H.A., Teunissen P.J.M., Yao J., Ward M. J. Biol. Chem. 278:31988-31997(2003) [PubMed: 12788920] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA], INDUCTION. Strain: QM6a.

[2]

"The first structure of a glycoside hydrolase family 61 member, Cel61B from Hypocrea jecorina, at 1.6 A resolution." Karkehabadi S., Hansson H., Kim S., Piens K., Mitchinson C., Sandgren M. J. Mol. Biol. 383:144-154(2008) [PubMed: 18723026] [Abstract] Cited for: X-RAY CRYSTALLOGRAPHY (1.6 ANGSTROMS) OF 20-249, SUBUNIT, CATALYTIC ACTIVITY, SUBCELLULAR LOCATION. Strain: QM6a.

Cross-references

Sequence databases

EMBLGenBankDDBJ

AY281372 mRNA. Translation: AAP57753.1.

3D structure databases

PDBPDBe

Entry	Method	Resolution (Å)	Chain	Positions	PDBsum
2VTC	X-ray	1.60	A/B	1-249	[»]

ModBase

Search...

Protein family/group databases

CAZy

GH61. Glycoside Hydrolase Family 61.

Family and domain databases

InterPro

IPR003961. FN_III.
IPR005103. Glyco_hydro_61.
[Graphical view]

Pfam

PF03443. Glyco_hydro_61. 1 hit.
[Graphical view]

SMART

SM00060. FN3. 1 hit.
[Graphical view]

ProtoNet

Search...

Entry information

Entry name

GUN7_TRIRE

Accession

Primary (citable) accession number: Q7Z9M7

Entry history

Integrated into UniProtKB/Swiss-Prot:	February 10, 2009
Last sequence update:	October 1, 2003
Last modified:	June 16, 2009
This is version 21 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

FPAP (Fungal Proteome Annotation Project)