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Q7Z9L3

- EXGA_ASPOR

UniProt

Q7Z9L3 - EXGA_ASPOR

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Protein

Glucan 1,3-beta-glucosidase A

Gene

exgA

Organism
Aspergillus oryzae (strain ATCC 42149 / RIB 40) (Yellow koji mold)
Status
Reviewed - Annotation score: 4 out of 5- Experimental evidence at protein leveli

Functioni

Beta-glucanases participate in the metabolism of beta-glucan, the main structural component of the cell wall. It could also function biosynthetically as a transglycosylase (By similarity).By similarity

Catalytic activityi

Successive hydrolysis of beta-D-glucose units from the non-reducing ends of (1->3)-beta-D-glucans, releasing alpha-glucose.

Cofactori

Mn2+1 Publication

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei199 – 1991Proton donorBy similarity
Active sitei298 – 2981NucleophileBy similarity

GO - Molecular functioni

  1. glucan exo-1,3-beta-glucosidase activity Source: ASPGD
  2. metal ion binding Source: UniProtKB-KW

GO - Biological processi

  1. carbohydrate metabolic process Source: ASPGD
  2. cell wall organization Source: UniProtKB-KW
  3. polysaccharide catabolic process Source: UniProtKB-KW
Complete GO annotation...

Keywords - Molecular functioni

Glycosidase, Hydrolase

Keywords - Biological processi

Carbohydrate metabolism, Cell wall biogenesis/degradation, Polysaccharide degradation

Keywords - Ligandi

Manganese, Metal-binding

Enzyme and pathway databases

BioCyciMetaCyc:MONOMER-16494.

Protein family/group databases

CAZyiGH5. Glycoside Hydrolase Family 5.
mycoCLAPiEXG5A_ASPOR.

Names & Taxonomyi

Protein namesi
Recommended name:
Glucan 1,3-beta-glucosidase A (EC:3.2.1.58)
Alternative name(s):
Exo-1,3-beta-glucanase 1
Exo-1,3-beta-glucanase A
Gene namesi
Name:exgA
Synonyms:exg1
ORF Names:AO090003000990
OrganismiAspergillus oryzae (strain ATCC 42149 / RIB 40) (Yellow koji mold)
Taxonomic identifieri510516 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaPezizomycotinaEurotiomycetesEurotiomycetidaeEurotialesAspergillaceaeAspergillus
ProteomesiUP000006564: Chromosome 2

Subcellular locationi

Secreted 1 Publication

GO - Cellular componenti

  1. extracellular region Source: UniProtKB-KW
Complete GO annotation...

Keywords - Cellular componenti

Secreted

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Signal peptidei1 – 1414Sequence AnalysisAdd
BLAST
Chaini15 – 405391Glucan 1,3-beta-glucosidase APRO_0000007876Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Disulfide bondi280 ↔ 405By similarity
Disulfide bondi306 ↔ 332By similarity

Keywords - PTMi

Disulfide bond

Expressioni

Inductioni

The combination of poor nutrition conditions and attachment of mycelia to a hydrophobic solid surface appears to be a major inducing factor.1 Publication

Interactioni

Subunit structurei

Monomer.1 Publication

Structurei

3D structure databases

ProteinModelPortaliQ7Z9L3.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Keywords - Domaini

Signal

Phylogenomic databases

eggNOGiCOG2730.
HOGENOMiHOG000114462.
OrthoDBiEOG7JT75H.

Family and domain databases

Gene3Di3.20.20.80. 1 hit.
InterProiIPR001547. Glyco_hydro_5.
IPR013781. Glyco_hydro_catalytic_dom.
IPR017853. Glycoside_hydrolase_SF.
[Graphical view]
PfamiPF00150. Cellulase. 1 hit.
[Graphical view]
SUPFAMiSSF51445. SSF51445. 1 hit.

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q7Z9L3-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MLPLLLCIVP YCWSSRLDPR ASSFDYNGEK VRGVNLGGWL VLEPWITPSI
60 70 80 90 100
FDAAGAEAVD EWSLTKILGK EEAEARLSAH WKSFVSAGDF QRMADAGLNH
110 120 130 140 150
VRIPIGYWAL GPLEGDPYVD GQLEYLDKAV EWAGAAGLKV LIDLHGAPGS
160 170 180 190 200
QNGFDNSGRR GAIQWQQGDT VEQTLDAFDL LAERYLGSDT VAAIEAINEP
210 220 230 240 250
NIPGGVDQGK LQEYYGSVYG IVNKYNAGTS VVYGDGFLPV ESWNGFKTEG
260 270 280 290 300
SKVVMDTHHY HMFDNGLIAM DIDSHIDAVC QFAHQHLEAS DKPVIVGEWT
310 320 330 340 350
GAVTDCAKYL NGKGNGARYD GSYAADKAIG DCSSLATGFV SKLSDEERSD
360 370 380 390 400
MRRFIEAQLD AFELKSGWVF WTWKTEGAPG WDMSDLLEAG VFPTSPDDRE

FPKQC
Length:405
Mass (Da):44,373
Last modified:October 1, 2003 - v1
Checksum:iFC8D181C0A37A7C9
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AJ566365 Genomic DNA. Translation: CAD97460.1.
AP007155 Genomic DNA. Translation: BAE58099.1.

Genome annotation databases

EnsemblFungiiCADAORAT00001553; CADAORAP00001533; CADAORAG00001553.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AJ566365 Genomic DNA. Translation: CAD97460.1 .
AP007155 Genomic DNA. Translation: BAE58099.1 .

3D structure databases

ProteinModelPortali Q7Z9L3.
ModBasei Search...
MobiDBi Search...

Protein family/group databases

CAZyi GH5. Glycoside Hydrolase Family 5.
mycoCLAPi EXG5A_ASPOR.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblFungii CADAORAT00001553 ; CADAORAP00001533 ; CADAORAG00001553 .

Phylogenomic databases

eggNOGi COG2730.
HOGENOMi HOG000114462.
OrthoDBi EOG7JT75H.

Enzyme and pathway databases

BioCyci MetaCyc:MONOMER-16494.

Family and domain databases

Gene3Di 3.20.20.80. 1 hit.
InterProi IPR001547. Glyco_hydro_5.
IPR013781. Glyco_hydro_catalytic_dom.
IPR017853. Glycoside_hydrolase_SF.
[Graphical view ]
Pfami PF00150. Cellulase. 1 hit.
[Graphical view ]
SUPFAMi SSF51445. SSF51445. 1 hit.
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "High glucose-tolerant beta-glucosidase gene from Aspergillus oryzae."
    Riou C., Gunata Z.
    Submitted (JUN-2003) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
  2. "Genome sequencing and analysis of Aspergillus oryzae."
    Machida M., Asai K., Sano M., Tanaka T., Kumagai T., Terai G., Kusumoto K., Arima T., Akita O., Kashiwagi Y., Abe K., Gomi K., Horiuchi H., Kitamoto K., Kobayashi T., Takeuchi M., Denning D.W., Galagan J.E.
    , Nierman W.C., Yu J., Archer D.B., Bennett J.W., Bhatnagar D., Cleveland T.E., Fedorova N.D., Gotoh O., Horikawa H., Hosoyama A., Ichinomiya M., Igarashi R., Iwashita K., Juvvadi P.R., Kato M., Kato Y., Kin T., Kokubun A., Maeda H., Maeyama N., Maruyama J., Nagasaki H., Nakajima T., Oda K., Okada K., Paulsen I., Sakamoto K., Sawano T., Takahashi M., Takase K., Terabayashi Y., Wortman J.R., Yamada O., Yamagata Y., Anazawa H., Hata Y., Koide Y., Komori T., Koyama Y., Minetoki T., Suharnan S., Tanaka A., Isono K., Kuhara S., Ogasawara N., Kikuchi H.
    Nature 438:1157-1161(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 42149 / RIB 40.
  3. "Purification, characterization, and substrate specificity of a novel highly glucose-tolerant beta-glucosidase from Aspergillus oryzae."
    Riou C., Salmon J.-M., Vallier M.-J., Guenata Z., Barre P.
    Appl. Environ. Microbiol. 64:3607-3614(1998) [PubMed] [Europe PMC] [Abstract]
    Cited for: COFACTOR, SUBUNIT, SUBCELLULAR LOCATION.
    Strain: ATCC 11489 / CBS 125.59 / IMI 52143 / NRRL 695.
  4. "The beta-1,3-exoglucanase gene exgA (exg1) of Aspergillus oryzae is required to catabolize extracellular glucan, and is induced in growth on a solid surface."
    Tamano K., Satoh Y., Ishii T., Terabayashi Y., Ohtaki S., Sano M., Takahashi T., Koyama Y., Mizutani O., Abe K., Machida M.
    Biosci. Biotechnol. Biochem. 71:926-934(2007) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, INDUCTION.

Entry informationi

Entry nameiEXGA_ASPOR
AccessioniPrimary (citable) accession number: Q7Z9L3
Secondary accession number(s): Q2UK16
Entry historyi
Integrated into UniProtKB/Swiss-Prot: April 13, 2004
Last sequence update: October 1, 2003
Last modified: November 26, 2014
This is version 77 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Glycosyl hydrolases
    Classification of glycosyl hydrolase families and list of entries
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3