Reviewed, UniProtKB/Swiss-Prot Q7Z8E7 (GPD_COLGL)

Names and origin

Protein names	Recommended name:     Glycerol-3-phosphate dehydrogenase [NAD+]     EC=1.1.1.8
Organism	Colletotrichum gloeosporioides (Anthracnose fungus) (Glomerella cingulata)
Taxonomic identifier	5457 [NCBI]
Taxonomic lineage	Eukaryota › Fungi › Dikarya › Ascomycota › Pezizomycotina › Sordariomycetes › Sordariomycetes incertae sedis › Phyllachorales › Phyllachoraceae › Glomerella

Protein attributes

Sequence length	420 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at transcript level.

General annotation (Comments)

Catalytic activity	sn-glycerol 3-phosphate + NAD(+) = glycerone phosphate + NADH.
Sequence similarities	Belongs to the NAD-dependent glycerol-3-phosphate dehydrogenase family.

Ontologies

Keywords
Ligand	NAD
Molecular function	Oxidoreductase
Gene Ontology (GO)
Biological process	carbohydrate metabolic process Inferred from electronic annotation. Source: InterPro glycerol-3-phosphate catabolic process Inferred from electronic annotation. Source: InterPro oxidation reduction Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	glycerol-3-phosphate dehydrogenase complex Inferred from electronic annotation. Source: InterPro
Molecular function	NAD binding Inferred from electronic annotation. Source: InterPro glycerol-3-phosphate dehydrogenase (NAD+) activity Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 420	420	Glycerol-3-phosphate dehydrogenase [NAD+]		PRO_0000138089
Regions
	Nucleotide binding	16 – 21	6	NAD By similarity
	Region	344 – 345	2	Substrate binding By similarity
Sites
	Active site	278	1	Proton acceptor By similarity
	Binding site	119	1	NAD By similarity
	Binding site	142	1	NAD; via amide nitrogen By similarity
	Binding site	142	1	Substrate By similarity
	Binding site	175	1	NAD; via amide nitrogen By similarity
	Binding site	344	1	NAD By similarity
	Binding site	373	1	NAD By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q7Z8E7-1 [UniParc]. Last modified October 1, 2003. Version 1. Checksum: ECCB6A3D3D1DF128	FASTA	420	45,278
	10 20 30 40 50 60 MASLGADKKH KVTVVGSGNW GSTICKIVAE NTKANPDLFE EAVHMWVFEE DVVIDKSSPY 70 80 90 100 110 120 YDPAVGDAPQ KLTGVINKYH ENTKYLPGIK LPDNIIANPS LQDAVKDSTI LVFNLPHQFI 130 140 150 160 170 180 GNVCKQLRGH IMPFARGISC IKGVNVSDDG ISLFSEWIGD GLGIYCGALS GANIASEIAA 190 200 210 220 230 240 EKWSETTIAY DPPPMDNSRA PTPRSNSPAN GNGIAPLTPV EMQHKDARGR TSKTKLTPVP 250 260 270 280 290 300 AEYPPLDHQI FKQLFHRPYF HVRMVSDVAG VSLGGALKNI VALAAGFVDG RGWGDNAKAA 310 320 330 340 350 360 IMRVGLLEMV NFGKEFFGQT VHTGTFTEES AGVADLITSC SGGRNFRCAK MAVAEGLSVE 370 380 390 400 410 420 EIEKRELNGQ LLQGTSTAKE VNSFLKARGL EKDYPLFTAV HGILEGRHSV DDIPSLVSDS

References

[1]

"Targeted gene disruption of glycerol-3-phosphate dehydrogenase in Colletotrichum gloeosporioides reveals evidence that glycerol is a significant transferred nutrient from host plant to fungal pathogen." Wei Y., Shen W., Dauk M., Wang F., Selvaraj G., Zou J. J. Biol. Chem. 279:429-435(2004) [PubMed: 14563847] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].

Cross-references

Sequence databases
EMBLGenBankDDBJ	AY331190 mRNA. Translation: AAP94992.1.
3D structure databases
ModBase	Search...
Family and domain databases
InterPro	IPR013328. DHase_multihelical. IPR016040. NAD(P)-bd. IPR017751. NAD-dep_Gly3P_DH_euk. IPR006168. NAD-dep_Gly3P_DHase. IPR011128. NAD-dep_Gly3P_DHase_N. IPR006109. NAD_Gly3P_DHase_C. [Graphical view]
Gene3D	G3DSA:3.40.50.720. NAD(P)-bd. 1 hit. G3DSA:1.10.1040.10. Opine_DH. 1 hit.
PANTHER	PTHR11728. NAD_Gly3P_DH. 1 hit.
Pfam	PF07479. NAD_Gly3P_dh_C. 1 hit. PF01210. NAD_Gly3P_dh_N. 1 hit. [Graphical view]
PIRSF	PIRSF000114. Glycerol-3-P_dh. 1 hit.
PRINTS	PR00077. GPDHDRGNASE.
ProDom	PD001278. NAD_Gly3P_C. 1 hit. [Graphical view] [Entries sharing at least one domain]
PROSITE	PS00957. NAD_G3PDH. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

GPD_COLGL

Accession

Primary (citable) accession number: Q7Z8E7

Entry history

Integrated into UniProtKB/Swiss-Prot:	January 10, 2006
Last sequence update:	October 1, 2003
Last modified:	November 25, 2008
This is version 29 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

FPAP (Fungal Proteome Annotation Project)