Reviewed, UniProtKB/Swiss-Prot Q7Z892 (PYRD1_SACKL)

Names and origin

Protein names

Recommended name:
    Dihydroorotate dehydrogenase
      Short name=DHOdehase
      Short name=DHODase
      Short name=DHOD
    EC=1.3.3.1
Alternative name(s):
    Dihydroorotate oxidase

Gene names

Name:

URA1

Organism

Saccharomyces kluyveri (Yeast) (Saccharomyces silvestris)

Taxonomic identifier

4934 [NCBI]

Taxonomic lineage

Eukaryota › Fungi › Dikarya › Ascomycota › Saccharomycotina › Saccharomycetes › Saccharomycetales › Saccharomycetaceae › Lachancea

Protein attributes

Sequence length	314 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at protein level.

General annotation (Comments)

Catalytic activity	(S)-dihydroorotate + O2 = orotate + H2O2.
Cofactor	Binds 1 FMN per subunit By similarity.
Enzyme regulation	The activity is independent of the presence of oxygen.
Pathway	Pyrimidine metabolism; UMP biosynthesis via de novo pathway; orotate from (S)-dihydroorotate (O2 route): step 1/1.
Subunit structure	Homodimer By similarity.
Subcellular location	Cytoplasm By similarity.
Miscellaneous	S.kluyveri has two isoforms of DHODase, a cytoplasmic isoform and a mitochondrial isoform.
Sequence similarities	Belongs to the dihydroorotate dehydrogenase family. Type 1 subfamily.

Ontologies

Keywords
Biological process	Pyrimidine biosynthesis
Cellular component	Cytoplasm
Ligand	FMN Flavoprotein
Molecular function	Oxidoreductase
Gene Ontology (GO)
Biological process	'de novo' pyrimidine base biosynthetic process Inferred from electronic annotation. Source: InterPro UMP biosynthetic process Inferred from electronic annotation. Source: InterPro oxidation reduction Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	dihydroorotate oxidase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 314	314	Dihydroorotate dehydrogenase		PRO_0000148503
Sites
	Active site	133	1	Nucleophile By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q7Z892-1 [UniParc]. Last modified October 1, 2003. Version 1. Checksum: C7501535D7800D84	FASTA	314	34,855
	10 20 30 40 50 60 MSASLAINFL NHTYENPFMN ASGVHCMSTK ELDELKDSRA GAFITKSSTT SKREGNPEPR 70 80 90 100 110 120 YFSVPLGSIN SMGLPNEGFD YYLKYALEYQ KNGSTSTPLF FSVAGMSVEE NLKMLQKIQD 130 140 150 160 170 180 SDFNGITELN LSCPNVPGKP QVAYDFELTK EILTKVFEFF KKPLGVKLPP YFDFAHFDIM 190 200 210 220 230 240 AGILNQLPLS YVNCINSIGN GLYINVETES VVVKPKNGFG GIGGEYVKPT ALANVRAFYT 250 260 270 280 290 300 RLNPTIKIIG TGGIKTGQDA FEHLLCGATM LQVGTELYKE GVSIFDRLER ELKELMDKKG 310 YTSIEQFRGK LNSL

References

[1]	"Contribution of horizontal gene transfer to the evolution of Saccharomyces cerevisiae." Hall C.R., Brachat S., Dietrich F.S. Eukaryot. Cell 4:1102-1115(2005) [PubMed: 15947202] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: ATCC 58438 / CBS 3082 / IFO 1685 / JCM 7257 / NRRL Y-12651.
[2]	"Horizontal gene transfer promoted evolution of the ability to propagate under anaerobic conditions in yeasts." Gojkovic Z., Knecht W., Zameitat E., Warneboldt J., Coutelis J.-B., Pynyaha Y., Neuveglise C., Moeller K., Loeffler M., Piskur J. Mol. Genet. Genomics 271:387-393(2004) [PubMed: 15014982] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], CHARACTERIZATION.

Cross-references

Sequence databases
EMBLGenBankDDBJ	AY323902 Genomic DNA. Translation: AAQ01779.1. AF452109 Genomic DNA. Translation: AAQ04683.1.
3D structure databases
SMR	Q7Z892. Positions 5-314.
ModBase	Search...
Enzyme and pathway databases
BRENDA	1.3.3.1. 274780.
Family and domain databases
InterPro	IPR013785. Aldolase_TIM. IPR012135. Dihydroorotate_DH_1_2. IPR005720. Dihydroorotate_DH_1_core. IPR001295. Dihydroorotate_DH_CS. [Graphical view]
Gene3D	G3DSA:3.20.20.70. Aldolase_TIM. 1 hit.
Pfam	PF01180. DHO_dh. 1 hit. [Graphical view]
PIRSF	PIRSF000164. DHO_oxidase. 1 hit.
TIGRFAMs	TIGR01037. pyrD_sub1_fam. 1 hit.
PROSITE	PS00911. DHODEHASE_1. 1 hit. PS00912. DHODEHASE_2. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

PYRD1_SACKL

Accession

Primary (citable) accession number: Q7Z892

Entry history

Integrated into UniProtKB/Swiss-Prot:	August 31, 2004
Last sequence update:	October 1, 2003
Last modified:	September 1, 2009
This is version 43 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

FPAP (Fungal Proteome Annotation Project)