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Q7Z7W5 (DNLI4_COPCI) Reviewed, UniProtKB/Swiss-Prot

Last modified May 31, 2011. Version 45. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (1) | Third-party data text xml rdf/xml gff fasta
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Names and origin

Protein namesRecommended name:
DNA ligase 4
EC=6.5.1.1
Alternative name(s):
DNA ligase IV
Polydeoxyribonucleotide synthase [ATP] 4
Gene names
Name:LIG4
OrganismCoprinopsis cinerea (Inky cap fungus) (Hormographiella aspergillata)
Taxonomic identifier5346 [NCBI]
Taxonomic lineageEukaryota › Fungi › Dikarya › Basidiomycota › Agaricomycotina › Homobasidiomycetes › Agaricomycetidae › Agaricales › Psathyrellaceae › Coprinopsis

Protein attributes

Sequence length1025 AA.
Sequence statusComplete.
Protein existenceEvidence at transcript level

General annotation (Comments)

Function

Involved in ds DNA break repair. Has a role in non-homologous integration (NHI) pathways where it is required in the final step of non-homologus end-joining. Ref.1

Catalytic activity

ATP + (deoxyribonucleotide)(n) + (deoxyribonucleotide)(m) = AMP + diphosphate + (deoxyribonucleotide)(n+m).

Cofactor

Magnesium By similarity.

Subcellular location

Nucleus By similarity.

Sequence similarities

Belongs to the ATP-dependent DNA ligase family.

Contains 2 BRCT domains.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 10251025DNA ligase 4
PRO_0000278380

Regions

Domain667 – 76397BRCT 1
Domain915 – 1025111BRCT 2

Sites

Active site2911N6-AMP-lysine intermediate By similarity
Metal binding3491Magnesium 1 Potential
Metal binding4471Magnesium 2 Potential
Binding site2891ATP By similarity
Binding site2961ATP By similarity
Binding site3111ATP By similarity
Binding site4521ATP By similarity
Binding site4631ATP By similarity
Binding site4691ATP By similarity

Sequences

Sequence LengthMass (Da)Tools
Q7Z7W5 [UniParc].

Last modified October 1, 2003. Version 1.
Checksum: ACAE9D076932868C

FASTA1,025116,825
        10         20         30         40         50         60 
MMQPTPAPSS APGSPQRTQA EPEMETPSYP QPPQNVGTAP FSVLVKLFEK LATERKQERR 

        70         80         90        100        110        120 
RKLLDAWFRH WRREKGFDLY PVLRLLLPQK DRDRAVYGLK EKNLAKTYIK LIPLGMRDPD 

       130        140        150        160        170        180 
AIRLLNWKKP TERDKSSGDF PQVLCEVVSK RSSVIEGTLT IDELNEILDD IAKNMGKSDV 

       190        200        210        220        230        240 
QSKILRRIYN NSTADEQRWI IRIILKDMNI SVKETTVFAV FHPDAQDLYN TCSDLKKVAW 

       250        260        270        280        290        300 
ELWDPSRRLN AKDKEIQIFH AFAPMLCKRP TRKIEETVKA MGGSKFIIEE KLDGERMQLH 

       310        320        330        340        350        360 
KRGNEYFYCS RKGKDYTYLY GKHIGAGSLT PFIDSAFDSR IDDIILDGEM LVWDPVSERN 

       370        380        390        400        410        420 
LPFGTLKTAA LDRSKKENNP RPCFKVFDLL YLNGMSLLDK TVKFRKNNLR HCIKPIPGRI 

       430        440        450        460        470        480 
EFVEEYQGET ANDIRKRMEQ VMENRGEGLV IKHPKAKYIL NGRNTDWIKV KPEYMDNMGE 

       490        500        510        520        530        540 
TVDVLVVAGN YGSGKRGGGV STLICAVMDD RRPDSDDEPK YSSFVRIGTG LSFADYVWVR 

       550        560        570        580        590        600 
SKPWKVWDPK NPPEFLQTAK KGQEDKGDVY LEPEDSFILK VKAAEITPSD QYHMGFTMRF 

       610        620        630        640        650        660 
PRALAIRDDL SIADCMTATE VFESLKSERK RKMEDDAGIT TKKRKTTVKK VALLPEYSGP 

       670        680        690        700        710        720 
NLKKVAVKTD IFNGMKFVVF SDPKSRTGEA DKKELMKTIH ANGGTCSQIV NKNSEAIVIY 

       730        740        750        760        770        780 
GGSITPYDLK LVIDKGIHDV IKPSWITDSV TLGEPAPFKK KYFFHATEER KYADEYNEDD 

       790        800        810        820        830        840 
GEEEGAVPSA DEQERDVKSG TVEPGSETED EDEEQAPEIK EEQDGELHEW LKVDDRKSPA 

       850        860        870        880        890        900 
LPAHDEEDSV TEDDSDNADV ADEEEPDLDD WFQVKGETED EGAGALATAS RHRETTPDVD 

       910        920        930        940        950        960 
GDVKMGESEE AMDYDPDVIF KHLCFYLDSP ANAQRHGMAT RPKYEAAITK SFEEVEKLIK 

       970        980        990       1000       1010       1020 
DNGGKIVDLD EPKLTHVVLD KRDDSRRVEL MKRTSKPRRR HLVLSDYIEA CIDEGTLLDE 


EEFAP

« Hide

References

[1]"DNA ligase IV from a basidiomycete, Coprinus cinereus, and its expression during meiosis."
Namekawa S., Ichijima Y., Hamada F., Kasai N., Iwabata K., Nara T., Teraoka H., Sugawara F., Sakaguchi K.
Microbiology 149:2119-2128(2003) [PubMed: 12904551] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA], FUNCTION.
Strain: ATCC 56838.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ		AB098474 mRNA. Translation: BAC76766.1.
AB072455 mRNA. Translation: BAD93669.1.

3D structure databases

ProteinModelPortalQ7Z7W5.
ModBaseSearch...

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Family and domain databases

InterProIPR001357. BRCT.
IPR000977. DNA_ligase_ATP-dep.
IPR012309. DNA_ligase_ATP-dep_C.
IPR012310. DNA_ligase_ATP-dep_cent.
IPR016059. DNA_ligase_ATP-dep_CS.
IPR012308. DNA_ligase_ATP-dep_N.
IPR012340. NA-bd_OB-fold.
IPR016027. NA-bd_OB-fold-like.
[Graphical view]
Gene3DG3DSA:1.10.3260.10. DNA_ligase_ATP-dep_N. 1 hit.
G3DSA:2.40.50.140. OB_NA_bd_sub. 1 hit.
PfamPF00533. BRCT. 1 hit.
PF04679. DNA_ligase_A_C. 1 hit.
PF01068. DNA_ligase_A_M. 1 hit.
PF04675. DNA_ligase_A_N. 1 hit.
[Graphical view]
SMARTSM00292. BRCT. 2 hits.
[Graphical view]
SUPFAMSSF52113. BRCT. 1 hit.
SSF50249. Nucleic_acid_OB. 1 hit.
SSF117018. SSF117018. 1 hit.
TIGRFAMsTIGR00574. Dnl1. 1 hit.
PROSITEPS50172. BRCT. 2 hits.
PS00697. DNA_LIGASE_A1. 1 hit.
PS00333. DNA_LIGASE_A2. 1 hit.
PS50160. DNA_LIGASE_A3. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameDNLI4_COPCI
AccessionPrimary (citable) accession number: Q7Z7W5
Entry history
Integrated into UniProtKB/Swiss-Prot: February 20, 2007
Last sequence update: October 1, 2003
Last modified: May 31, 2011
This is version 45 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

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