Q7Z7M8 (B3GN8_HUMAN) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
UDP-GlcNAc:betaGal beta-1,3-N-acetylglucosaminyltransferase 8
Short name=BGnT-8
Short name=Beta-1,3-Gn-T8
Short name=Beta-1,3-N-acetylglucosaminyltransferase 8
Short name=Beta3Gn-T8
EC=2.4.1.-

Gene names

Name:	B3GNT8
Synonyms:	B3GALT7, BGALT15

Organism

Homo sapiens (Human)

Taxonomic identifier

9606 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Primates › Haplorrhini › Catarrhini › Hominidae › Homo

Protein attributes

Sequence length	397 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Beta-1,3-N-acetylglucosaminyltransferase that plays a role in the elongation of specific branch structures of multiantennary N-glycans. Has strong activity towards tetraantennary N-glycans and 2,6 triantennary glycans. Ref.2 Ref.4
Pathway	Protein modification; protein glycosylation.
Subunit structure	Forms a complex of enhanced enzymatic activity with B3GNT2 in vitro. Ref.4
Subcellular location	Golgi apparatus membrane; Single-pass type II membrane protein By similarity UniProtKB Q9NY97.
Tissue specificity	Highly expressed in small intestine, pancreas, spleen, bone marrow, lung, throat, and ileum, and weakly in fetal brain, cerebellum, heart, liver, tongue, breast, uteri, and testis. Not detected in colon. Differentially expressed in human tumor cell lines. Ref.1 Ref.2
Induction	Up-regulated in colon cancer. Ref.2
Sequence similarities	Belongs to the glycosyltransferase 31 family.

Ontologies

Keywords
Cellular component	Golgi apparatus Membrane
Coding sequence diversity	Polymorphism
Domain	Signal-anchor Transmembrane Transmembrane helix
Molecular function	Glycosyltransferase Transferase
PTM	Glycoprotein
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological process	poly-N-acetyllactosamine biosynthetic process Inferred from direct assay Ref.2. Source: HGNC protein glycosylation Inferred from electronic annotation. Source: InterPro
Cellular component	Golgi membrane Inferred from electronic annotation. Source: UniProtKB-SubCell integral to membrane Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	galactosyltransferase activity Inferred from electronic annotation. Source: InterPro protein N-acetylglucosaminyltransferase activity Inferred from direct assay Ref.2. Source: HGNC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 397	397	UDP-GlcNAc:betaGal beta-1,3-N-acetylglucosaminyltransferase 8		PRO_0000306381
Regions
	Topological domain	1 – 6	6	Cytoplasmic Potential
	Transmembrane	7 – 23	17	Helical; Signal-anchor for type II membrane protein; Potential
	Topological domain	24 – 397	374	Lumenal Potential
	Compositional bias	99 – 102	4	Poly-Ala
	Compositional bias	133 – 136	4	Poly-Gly
	Compositional bias	226 – 229	4	Poly-Leu
Amino acid modifications
	Glycosylation	57	1	N-linked (GlcNAc...) Potential
Natural variations
	Natural variant	137	1	S → G. Corresponds to variant rs284662 [ dbSNP | Ensembl ].		VAR_049348

Sequences

	Sequence		Length	Mass (Da)
	Q7Z7M8 [UniParc]. Last modified October 1, 2003. Version 1. Checksum: AC0CCE6880F46850	FASTA	397	43,396
	10 20 30 40 50 60 MRCPKCLLCL SALLTLLGLK VYIEWTSESR LSKAYPSPRG TPPSPTPANP EPTLPANLST 70 80 90 100 110 120 RLGQTIPLPF AYWNQQQWRL GSLPSGDSTE TGGCQAWGAA AATEIPDFAS YPKDLRRFLL 130 140 150 160 170 180 SAACRSFPQW LPGGGGSQVS SCSDTDVPYL LLAVKSEPGR FAERQAVRET WGSPAPGIRL 190 200 210 220 230 240 LFLLGSPVGE AGPDLDSLVA WESRRYSDLL LWDFLDVPFN QTLKDLLLLA WLGRHCPTVS 250 260 270 280 290 300 FVLRAQDDAF VHTPALLAHL RALPPASARS LYLGEVFTQA MPLRKPGGPF YVPESFFEGG 310 320 330 340 350 360 YPAYASGGGY VIAGRLAPWL LRAAARVAPF PFEDVYTGLC IRALGLVPQA HPGFLTAWPA 370 380 390 DRTADHCAFR NLLLVRPLGP QASIRLWKQL QDPRLQC

References

[1]	"Cloning and tissue distribution of the human B3GALT7 gene, a member of the beta1,3-Glycosyltransferase family." Huang C., Zhou J., Wu S., Shan Y., Teng S., Yu L. Glycoconj. J. 21:267-273(2004) [PubMed: 15486459] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA], TISSUE SPECIFICITY. Tissue: Lung.
[2]	"A novel beta1,3-N-acetylglucosaminyltransferase (beta3Gn-T8), which synthesizes poly-N-acetyllactosamine, is dramatically upregulated in colon cancer." Ishida H., Togayachi A., Sakai T., Iwai T., Hiruma T., Sato T., Okubo R., Inaba N., Kudo T., Gotoh M., Shoda J., Tanaka N., Narimatsu H. FEBS Lett. 579:71-78(2005) [PubMed: 15620693] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA], FUNCTION, TISSUE SPECIFICITY, INDUCTION, ENZYME ACTIVITY. Tissue: Colon cancer.
[3]	"Beta1,3 GlcNAc-Transferase-T7, core 3 synthase." Bennett E.P. Submitted (NOV-2001) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [MRNA].
[4]	"Characterization of a novel galactose beta1,3-N-acetylglucosaminyltransferase (beta3Gn-T8): the complex formation of beta3Gn-T2 and beta3Gn-T8 enhances enzymatic activity." Seko A., Yamashita K. Glycobiology 15:943-951(2005) [PubMed: 15917431] [Abstract] Cited for: FUNCTION, SUBUNIT, ENZYME ACTIVITY.
+	Additional computationally mapped references.

Web resources

GGDB

GlycoGene database

Cross-references

Sequence databases
EMBL GenBank DDBJ	AY277592 mRNA. Translation: AAP34405.1. AB175895 mRNA. Translation: BAD86525.1. AJ419172 mRNA. Translation: CAD11601.1.
IPI	IPI00184094.
RefSeq	NP_940942.1. NM_198540.2.
UniGene	Hs.441681.
3D structure databases
ProteinModelPortal	Q7Z7M8.
ModBase	Search...
Protein family/group databases
CAZy	GT31. Glycosyltransferase Family 31.
Polymorphism databases
DMDM	74713777.
Proteomic databases
PRIDE	Q7Z7M8.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
Ensembl	ENST00000321702; ENSP00000312700; ENSG00000177191.
GeneID	374907.
KEGG	hsa:374907.
UCSC	uc002oqs.1. human.
Organism-specific databases
CTD	374907.
GeneCards	GC19M041935.
H-InvDB	HIX0039940.
HGNC	HGNC:24139. B3GNT8.
neXtProt	NX_Q7Z7M8.
PharmGKB	PA134910679.
GenAtlas	Search...
Phylogenomic databases
eggNOG	prNOG19346.
GeneTree	ENSGT00560000076821.
HOGENOM	HBG714704.
HOVERGEN	HBG050653.
InParanoid	Q7Z7M8.
OMA	AACRSFP.
OrthoDB	EOG45HRXS.
PhylomeDB	Q7Z7M8.
Gene expression databases
ArrayExpress	Q7Z7M8.
Bgee	Q7Z7M8.
CleanEx	HS_B3GNT8.
Genevestigator	Q7Z7M8.
Family and domain databases
InterPro	IPR002659. Glyco_trans_31. [Graphical view]
KO	K09665.
PANTHER	PTHR11214. Glyco_trans_31. 1 hit.
Pfam	PF01762. Galactosyl_T. 1 hit. [Graphical view]
ProtoNet	Search...
Other
NextBio	100329.

Entry information

Entry name

B3GN8_HUMAN

Accession

Primary (citable) accession number: Q7Z7M8

Entry history

Integrated into UniProtKB/Swiss-Prot:	October 2, 2007
Last sequence update:	October 1, 2003
Last modified:	January 25, 2012
This is version 60 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program

Disclaimer

Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.