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Protein

UDP-GlcNAc:betaGal beta-1,3-N-acetylglucosaminyltransferase 8

Gene

B3GNT8

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Beta-1,3-N-acetylglucosaminyltransferase that plays a role in the elongation of specific branch structures of multiantennary N-glycans. Has strong activity towards tetraantennary N-glycans and 2,6 triantennary glycans.2 Publications

Pathwayi: protein glycosylation

This protein is involved in the pathway protein glycosylation, which is part of Protein modification.Curated
View all proteins of this organism that are known to be involved in the pathway protein glycosylation and in Protein modification.

GO - Molecular functioni

GO - Biological processi

  • O-glycan processing Source: Reactome
  • poly-N-acetyllactosamine biosynthetic process Source: HGNC
Complete GO annotation...

Keywords - Molecular functioni

Glycosyltransferase, Transferase

Enzyme and pathway databases

ReactomeiR-HSA-913709. O-linked glycosylation of mucins.
UniPathwayiUPA00378.

Protein family/group databases

CAZyiGT31. Glycosyltransferase Family 31.

Names & Taxonomyi

Protein namesi
Recommended name:
UDP-GlcNAc:betaGal beta-1,3-N-acetylglucosaminyltransferase 8 (EC:2.4.1.-)
Short name:
BGnT-8
Short name:
Beta-1,3-Gn-T8
Short name:
Beta-1,3-N-acetylglucosaminyltransferase 8
Short name:
Beta3Gn-T8
Gene namesi
Name:B3GNT8Imported
Synonyms:B3GALT71 Publication, BGALT15Imported
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
Proteomesi
  • UP000005640 Componenti: Chromosome 19

Organism-specific databases

HGNCiHGNC:24139. B3GNT8.

Subcellular locationi

  • Golgi apparatus membrane By similarity; Single-pass type II membrane protein By similarity

Topology

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Topological domaini1 – 66CytoplasmicSequence analysis
Transmembranei7 – 2317Helical; Signal-anchor for type II membrane proteinSequence analysisAdd
BLAST
Topological domaini24 – 397374LumenalSequence analysisAdd
BLAST

GO - Cellular componenti

  • extracellular exosome Source: UniProtKB
  • Golgi membrane Source: Reactome
  • integral component of membrane Source: UniProtKB-KW
Complete GO annotation...

Keywords - Cellular componenti

Golgi apparatus, Membrane

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Mutagenesisi246 – 2461Q → A: Loss of enzymatic activity, no loss of B3GNT2-binding and activation. 1 Publication

Organism-specific databases

PharmGKBiPA134910679.

Polymorphism and mutation databases

BioMutaiB3GNT8.
DMDMi74713777.

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 397397UDP-GlcNAc:betaGal beta-1,3-N-acetylglucosaminyltransferase 8PRO_0000306381Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Glycosylationi57 – 571N-linked (GlcNAc...)Sequence analysis

Keywords - PTMi

Glycoprotein

Proteomic databases

PaxDbiQ7Z7M8.
PRIDEiQ7Z7M8.

PTM databases

iPTMnetiQ7Z7M8.

Expressioni

Tissue specificityi

Highly expressed in small intestine, pancreas, spleen, bone marrow, lung, throat, and ileum, and weakly in fetal brain, cerebellum, heart, liver, tongue, breast, uteri, and testis. Not detected in colon. Differentially expressed in human tumor cell lines.2 Publications

Inductioni

Up-regulated in colon cancer.1 Publication

Gene expression databases

BgeeiQ7Z7M8.
CleanExiHS_B3GNT8.
GenevisibleiQ7Z7M8. HS.

Organism-specific databases

HPAiHPA043669.

Interactioni

Subunit structurei

Interacts with B3GNT2; this interaction greatly increases B3GNT2 catalytic activity, independently of B3GNT8 enzymatic activity.2 Publications

Protein-protein interaction databases

STRINGi9606.ENSP00000312700.

Structurei

3D structure databases

ProteinModelPortaliQ7Z7M8.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Compositional bias

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Compositional biasi99 – 1024Poly-AlaSequence analysis
Compositional biasi133 – 1364Poly-GlySequence analysis
Compositional biasi226 – 2294Poly-LeuSequence analysis

Sequence similaritiesi

Belongs to the glycosyltransferase 31 family.Sequence analysis

Keywords - Domaini

Signal-anchor, Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiKOG2287. Eukaryota.
ENOG410ZZ1B. LUCA.
GeneTreeiENSGT00760000118879.
HOGENOMiHOG000232195.
HOVERGENiHBG050653.
InParanoidiQ7Z7M8.
KOiK09665.
OMAiYIEWTSE.
OrthoDBiEOG7BGHKT.
PhylomeDBiQ7Z7M8.
TreeFamiTF318639.

Family and domain databases

InterProiIPR002659. Glyco_trans_31.
[Graphical view]
PANTHERiPTHR11214. PTHR11214. 1 hit.
PfamiPF01762. Galactosyl_T. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q7Z7M8-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MRCPKCLLCL SALLTLLGLK VYIEWTSESR LSKAYPSPRG TPPSPTPANP
60 70 80 90 100
EPTLPANLST RLGQTIPLPF AYWNQQQWRL GSLPSGDSTE TGGCQAWGAA
110 120 130 140 150
AATEIPDFAS YPKDLRRFLL SAACRSFPQW LPGGGGSQVS SCSDTDVPYL
160 170 180 190 200
LLAVKSEPGR FAERQAVRET WGSPAPGIRL LFLLGSPVGE AGPDLDSLVA
210 220 230 240 250
WESRRYSDLL LWDFLDVPFN QTLKDLLLLA WLGRHCPTVS FVLRAQDDAF
260 270 280 290 300
VHTPALLAHL RALPPASARS LYLGEVFTQA MPLRKPGGPF YVPESFFEGG
310 320 330 340 350
YPAYASGGGY VIAGRLAPWL LRAAARVAPF PFEDVYTGLC IRALGLVPQA
360 370 380 390
HPGFLTAWPA DRTADHCAFR NLLLVRPLGP QASIRLWKQL QDPRLQC
Length:397
Mass (Da):43,396
Last modified:October 1, 2003 - v1
Checksum:iAC0CCE6880F46850
GO

Natural variant

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Natural varianti137 – 1371S → G.
Corresponds to variant rs284662 [ dbSNP | Ensembl ].
VAR_049348

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AY277592 mRNA. Translation: AAP34405.1.
AB175895 mRNA. Translation: BAD86525.1.
AJ419172 mRNA. Translation: CAD11601.1.
CCDSiCCDS12582.1.
RefSeqiNP_940942.1. NM_198540.2.
XP_011525236.1. XM_011526934.1.
UniGeneiHs.441681.

Genome annotation databases

EnsembliENST00000321702; ENSP00000312700; ENSG00000177191.
GeneIDi374907.
KEGGihsa:374907.
UCSCiuc002oqs.3. human.

Keywords - Coding sequence diversityi

Polymorphism

Cross-referencesi

Web resourcesi

GGDB

GlycoGene database

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AY277592 mRNA. Translation: AAP34405.1.
AB175895 mRNA. Translation: BAD86525.1.
AJ419172 mRNA. Translation: CAD11601.1.
CCDSiCCDS12582.1.
RefSeqiNP_940942.1. NM_198540.2.
XP_011525236.1. XM_011526934.1.
UniGeneiHs.441681.

3D structure databases

ProteinModelPortaliQ7Z7M8.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi9606.ENSP00000312700.

Protein family/group databases

CAZyiGT31. Glycosyltransferase Family 31.

PTM databases

iPTMnetiQ7Z7M8.

Polymorphism and mutation databases

BioMutaiB3GNT8.
DMDMi74713777.

Proteomic databases

PaxDbiQ7Z7M8.
PRIDEiQ7Z7M8.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENST00000321702; ENSP00000312700; ENSG00000177191.
GeneIDi374907.
KEGGihsa:374907.
UCSCiuc002oqs.3. human.

Organism-specific databases

CTDi374907.
GeneCardsiB3GNT8.
HGNCiHGNC:24139. B3GNT8.
HPAiHPA043669.
MIMi615357. gene.
neXtProtiNX_Q7Z7M8.
PharmGKBiPA134910679.
GenAtlasiSearch...

Phylogenomic databases

eggNOGiKOG2287. Eukaryota.
ENOG410ZZ1B. LUCA.
GeneTreeiENSGT00760000118879.
HOGENOMiHOG000232195.
HOVERGENiHBG050653.
InParanoidiQ7Z7M8.
KOiK09665.
OMAiYIEWTSE.
OrthoDBiEOG7BGHKT.
PhylomeDBiQ7Z7M8.
TreeFamiTF318639.

Enzyme and pathway databases

UniPathwayiUPA00378.
ReactomeiR-HSA-913709. O-linked glycosylation of mucins.

Miscellaneous databases

GenomeRNAii374907.
PROiQ7Z7M8.
SOURCEiSearch...

Gene expression databases

BgeeiQ7Z7M8.
CleanExiHS_B3GNT8.
GenevisibleiQ7Z7M8. HS.

Family and domain databases

InterProiIPR002659. Glyco_trans_31.
[Graphical view]
PANTHERiPTHR11214. PTHR11214. 1 hit.
PfamiPF01762. Galactosyl_T. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

  1. "Cloning and tissue distribution of the human B3GALT7 gene, a member of the beta1,3-Glycosyltransferase family."
    Huang C., Zhou J., Wu S., Shan Y., Teng S., Yu L.
    Glycoconj. J. 21:267-273(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA], TISSUE SPECIFICITY.
    Tissue: Lung1 Publication.
  2. "A novel beta1,3-N-acetylglucosaminyltransferase (beta3Gn-T8), which synthesizes poly-N-acetyllactosamine, is dramatically upregulated in colon cancer."
    Ishida H., Togayachi A., Sakai T., Iwai T., Hiruma T., Sato T., Okubo R., Inaba N., Kudo T., Gotoh M., Shoda J., Tanaka N., Narimatsu H.
    FEBS Lett. 579:71-78(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA], FUNCTION, TISSUE SPECIFICITY, INDUCTION, ENZYME ACTIVITY.
    Tissue: Colon cancerImported.
  3. "Beta1,3 GlcNAc-Transferase-T7, core 3 synthase."
    Bennett E.P.
    Submitted (NOV-2001) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
  4. "Characterization of a novel galactose beta1,3-N-acetylglucosaminyltransferase (beta3Gn-T8): the complex formation of beta3Gn-T2 and beta3Gn-T8 enhances enzymatic activity."
    Seko A., Yamashita K.
    Glycobiology 15:943-951(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, INTERACTION WITH B3GNT2, ENZYME ACTIVITY.
  5. "Activation of beta1,3-N-acetylglucosaminyltransferase-2 (beta3Gn-T2) by beta3Gn-T8. Possible involvement of beta3Gn-T8 in increasing poly-N-acetyllactosamine chains in differentiated HL-60 cells."
    Seko A., Yamashita K.
    J. Biol. Chem. 283:33094-33100(2008) [PubMed] [Europe PMC] [Abstract]
    Cited for: INTERACTION WITH B3GNT2, MUTAGENESIS OF GLN-246.

Entry informationi

Entry nameiB3GN8_HUMAN
AccessioniPrimary (citable) accession number: Q7Z7M8
Entry historyi
Integrated into UniProtKB/Swiss-Prot: October 2, 2007
Last sequence update: October 1, 2003
Last modified: June 8, 2016
This is version 90 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Human chromosome 19
    Human chromosome 19: entries, gene names and cross-references to MIM
  2. Human entries with polymorphisms or disease mutations
    List of human entries with polymorphisms or disease mutations
  3. Human polymorphisms and disease mutations
    Index of human polymorphisms and disease mutations
  4. MIM cross-references
    Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
  5. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  6. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.