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Protein

Transmembrane emp24 domain-containing protein 4

Gene

TMED4

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Involved in vesicular protein trafficking, mainly in the early secretory pathway. targeting. Involved in the maintenance of the Golgi apparatus. Appears to play a role in the biosynthesis of secreted cargo including processing. Involved in endoplasmic reticulum stress response. May play a role in the regulation of heat-shock response and apoptosis (By similarity).By similarity

GO - Molecular functioni

  • signal transducer activity Source: UniProtKB

GO - Biological processi

  • positive regulation of I-kappaB kinase/NF-kappaB signaling Source: UniProtKB
  • protein transport Source: UniProtKB-KW
  • signal transduction Source: GOC
Complete GO annotation...

Keywords - Biological processi

Protein transport, Transport

Names & Taxonomyi

Protein namesi
Recommended name:
Transmembrane emp24 domain-containing protein 4
Alternative name(s):
Endoplasmic reticulum stress-response protein 25
Short name:
ERS25
GMP25iso
Putative NF-kappa-B-activating protein 156
p24 family protein alpha-3
Short name:
p24alpha3
Gene namesi
Name:TMED4
Synonyms:ERS25
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
Proteomesi
  • UP000005640 Componenti: Chromosome 7

Organism-specific databases

HGNCiHGNC:22301. TMED4.

Subcellular locationi

Topology

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Topological domaini30 – 194165LumenalSequence analysisAdd
BLAST
Transmembranei195 – 21218HelicalSequence analysisAdd
BLAST
Topological domaini213 – 22715CytoplasmicSequence analysisAdd
BLAST

GO - Cellular componenti

  • endoplasmic reticulum membrane Source: UniProtKB-SubCell
  • extracellular exosome Source: UniProtKB
  • integral component of membrane Source: UniProtKB-KW
Complete GO annotation...

Keywords - Cellular componenti

Endoplasmic reticulum, Membrane

Pathology & Biotechi

Organism-specific databases

PharmGKBiPA134983854.

Polymorphism and mutation databases

BioMutaiTMED4.
DMDMi62287892.

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Signal peptidei1 – 2929Sequence analysisAdd
BLAST
Chaini30 – 227198Transmembrane emp24 domain-containing protein 4PRO_0000010387Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Glycosylationi117 – 1171N-linked (GlcNAc...)1 Publication

Keywords - PTMi

Glycoprotein

Proteomic databases

EPDiQ7Z7H5.
MaxQBiQ7Z7H5.
PaxDbiQ7Z7H5.
PRIDEiQ7Z7H5.

PTM databases

iPTMnetiQ7Z7H5.
PhosphoSiteiQ7Z7H5.
SwissPalmiQ7Z7H5.

Expressioni

Gene expression databases

BgeeiQ7Z7H5.
CleanExiHS_TMED4.
ExpressionAtlasiQ7Z7H5. baseline and differential.
GenevisibleiQ7Z7H5. HS.

Organism-specific databases

HPAiHPA014650.

Interactioni

Protein-protein interaction databases

BioGridi128780. 29 interactions.
IntActiQ7Z7H5. 5 interactions.
STRINGi9606.ENSP00000404042.

Structurei

3D structure databases

ProteinModelPortaliQ7Z7H5.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini39 – 13799GOLDPROSITE-ProRule annotationAdd
BLAST

Coiled coil

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Coiled coili147 – 17630Sequence analysisAdd
BLAST

Motif

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Motifi220 – 2278COPI vesicle coat-bindingSequence analysis
Motifi220 – 2212COPII vesicle coat-bindingSequence analysis

Sequence similaritiesi

Belongs to the EMP24/GP25L family.Curated
Contains 1 GOLD domain.PROSITE-ProRule annotation

Keywords - Domaini

Coiled coil, Signal, Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiKOG1690. Eukaryota.
ENOG410XPUW. LUCA.
GeneTreeiENSGT00430000030848.
HOGENOMiHOG000160228.
HOVERGENiHBG105357.
InParanoidiQ7Z7H5.
OMAiLGMHVEI.
OrthoDBiEOG74BJT2.
PhylomeDBiQ7Z7H5.
TreeFamiTF314123.

Family and domain databases

InterProiIPR009038. GOLD_dom.
IPR015720. TMP21-related.
[Graphical view]
PANTHERiPTHR22811. PTHR22811. 1 hit.
PfamiPF01105. EMP24_GP25L. 1 hit.
[Graphical view]
SMARTiSM01190. EMP24_GP25L. 1 hit.
[Graphical view]
PROSITEiPS50866. GOLD. 1 hit.
[Graphical view]

Sequences (3)i

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

This entry describes 3 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 1 (identifier: Q7Z7H5-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MAGVGAGPLR AMGRQALLLL ALCATGAQGL YFHIGETEKR CFIEEIPDET
60 70 80 90 100
MVIGNYRTQM WDKQKEVFLP STPGLGMHVE VKDPDGKVVL SRQYGSEGRF
110 120 130 140 150
TFTSHTPGDH QICLHSNSTR MALFAGGKLR VHLDIQVGEH ANNYPEIAAK
160 170 180 190 200
DKLTELQLRA RQLLDQVEQI QKEQDYQRYR EERFRLTSES TNQRVLWWSI
210 220
AQTVILILTG IWQMRHLKSF FEAKKLV
Length:227
Mass (Da):25,943
Last modified:October 1, 2003 - v1
Checksum:i3C650402F472A051
GO
Isoform 2 (identifier: Q7Z7H5-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     59-74: Missing.

Note: No experimental confirmation available.
Show »
Length:211
Mass (Da):24,070
Checksum:iD8111386449A8A53
GO
Isoform 3 (identifier: Q7Z7H5-3) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     179-186: YREERFRL → ASAYLLVI
     187-227: Missing.

Show »
Length:186
Mass (Da):20,781
Checksum:iDCEE9F5107DC4347
GO

Sequence cautioni

The sequence AAP20105.1 differs from that shown. Reason: Erroneous initiation. Translation N-terminally extended.Curated
The sequence BAC77362.1 differs from that shown. Reason: Erroneous initiation. Translation N-terminally extended.Curated

Alternative sequence

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Alternative sequencei59 – 7416Missing in isoform 2. 1 PublicationVSP_035698Add
BLAST
Alternative sequencei179 – 1868YREERFRL → ASAYLLVI in isoform 3. 2 PublicationsVSP_035699
Alternative sequencei187 – 22741Missing in isoform 3. 2 PublicationsVSP_035700Add
BLAST

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AK074557 mRNA. Translation: BAC11058.1.
AK290125 mRNA. Translation: BAF82814.1.
AK294122 mRNA. Translation: BAG57455.1.
BX647965 mRNA. No translation available.
CH236960 Genomic DNA. Translation: EAL23751.1.
CH471128 Genomic DNA. Translation: EAW61090.1.
CH471128 Genomic DNA. Translation: EAW61091.1.
BC052641 mRNA. Translation: AAH52641.1.
BC057851 mRNA. Translation: AAH57851.1.
AY191223 mRNA. Translation: AAP20105.1. Different initiation.
AB097009 mRNA. Translation: BAC77362.1. Different initiation.
CCDSiCCDS5493.1. [Q7Z7H5-1]
CCDS78227.1. [Q7Z7H5-3]
RefSeqiNP_001289987.1. NM_001303058.1. [Q7Z7H5-3]
NP_001289989.1. NM_001303060.1.
NP_872353.2. NM_182547.3. [Q7Z7H5-1]
UniGeneiHs.598832.

Genome annotation databases

EnsembliENST00000457408; ENSP00000404042; ENSG00000158604. [Q7Z7H5-1]
ENST00000481238; ENSP00000417443; ENSG00000158604. [Q7Z7H5-3]
GeneIDi222068.
KEGGihsa:222068.
UCSCiuc003tli.4. human. [Q7Z7H5-1]

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AK074557 mRNA. Translation: BAC11058.1.
AK290125 mRNA. Translation: BAF82814.1.
AK294122 mRNA. Translation: BAG57455.1.
BX647965 mRNA. No translation available.
CH236960 Genomic DNA. Translation: EAL23751.1.
CH471128 Genomic DNA. Translation: EAW61090.1.
CH471128 Genomic DNA. Translation: EAW61091.1.
BC052641 mRNA. Translation: AAH52641.1.
BC057851 mRNA. Translation: AAH57851.1.
AY191223 mRNA. Translation: AAP20105.1. Different initiation.
AB097009 mRNA. Translation: BAC77362.1. Different initiation.
CCDSiCCDS5493.1. [Q7Z7H5-1]
CCDS78227.1. [Q7Z7H5-3]
RefSeqiNP_001289987.1. NM_001303058.1. [Q7Z7H5-3]
NP_001289989.1. NM_001303060.1.
NP_872353.2. NM_182547.3. [Q7Z7H5-1]
UniGeneiHs.598832.

3D structure databases

ProteinModelPortaliQ7Z7H5.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi128780. 29 interactions.
IntActiQ7Z7H5. 5 interactions.
STRINGi9606.ENSP00000404042.

PTM databases

iPTMnetiQ7Z7H5.
PhosphoSiteiQ7Z7H5.
SwissPalmiQ7Z7H5.

Polymorphism and mutation databases

BioMutaiTMED4.
DMDMi62287892.

Proteomic databases

EPDiQ7Z7H5.
MaxQBiQ7Z7H5.
PaxDbiQ7Z7H5.
PRIDEiQ7Z7H5.

Protocols and materials databases

DNASUi222068.
Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENST00000457408; ENSP00000404042; ENSG00000158604. [Q7Z7H5-1]
ENST00000481238; ENSP00000417443; ENSG00000158604. [Q7Z7H5-3]
GeneIDi222068.
KEGGihsa:222068.
UCSCiuc003tli.4. human. [Q7Z7H5-1]

Organism-specific databases

CTDi222068.
GeneCardsiTMED4.
HGNCiHGNC:22301. TMED4.
HPAiHPA014650.
MIMi612038. gene.
neXtProtiNX_Q7Z7H5.
PharmGKBiPA134983854.
GenAtlasiSearch...

Phylogenomic databases

eggNOGiKOG1690. Eukaryota.
ENOG410XPUW. LUCA.
GeneTreeiENSGT00430000030848.
HOGENOMiHOG000160228.
HOVERGENiHBG105357.
InParanoidiQ7Z7H5.
OMAiLGMHVEI.
OrthoDBiEOG74BJT2.
PhylomeDBiQ7Z7H5.
TreeFamiTF314123.

Miscellaneous databases

ChiTaRSiTMED4. human.
GeneWikiiTMED4.
GenomeRNAii222068.
PROiQ7Z7H5.
SOURCEiSearch...

Gene expression databases

BgeeiQ7Z7H5.
CleanExiHS_TMED4.
ExpressionAtlasiQ7Z7H5. baseline and differential.
GenevisibleiQ7Z7H5. HS.

Family and domain databases

InterProiIPR009038. GOLD_dom.
IPR015720. TMP21-related.
[Graphical view]
PANTHERiPTHR22811. PTHR22811. 1 hit.
PfamiPF01105. EMP24_GP25L. 1 hit.
[Graphical view]
SMARTiSM01190. EMP24_GP25L. 1 hit.
[Graphical view]
PROSITEiPS50866. GOLD. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "Complete sequencing and characterization of 21,243 full-length human cDNAs."
    Ota T., Suzuki Y., Nishikawa T., Otsuki T., Sugiyama T., Irie R., Wakamatsu A., Hayashi K., Sato H., Nagai K., Kimura K., Makita H., Sekine M., Obayashi M., Nishi T., Shibahara T., Tanaka T., Ishii S.
    , Yamamoto J., Saito K., Kawai Y., Isono Y., Nakamura Y., Nagahari K., Murakami K., Yasuda T., Iwayanagi T., Wagatsuma M., Shiratori A., Sudo H., Hosoiri T., Kaku Y., Kodaira H., Kondo H., Sugawara M., Takahashi M., Kanda K., Yokoi T., Furuya T., Kikkawa E., Omura Y., Abe K., Kamihara K., Katsuta N., Sato K., Tanikawa M., Yamazaki M., Ninomiya K., Ishibashi T., Yamashita H., Murakawa K., Fujimori K., Tanai H., Kimata M., Watanabe M., Hiraoka S., Chiba Y., Ishida S., Ono Y., Takiguchi S., Watanabe S., Yosida M., Hotuta T., Kusano J., Kanehori K., Takahashi-Fujii A., Hara H., Tanase T.-O., Nomura Y., Togiya S., Komai F., Hara R., Takeuchi K., Arita M., Imose N., Musashino K., Yuuki H., Oshima A., Sasaki N., Aotsuka S., Yoshikawa Y., Matsunawa H., Ichihara T., Shiohata N., Sano S., Moriya S., Momiyama H., Satoh N., Takami S., Terashima Y., Suzuki O., Nakagawa S., Senoh A., Mizoguchi H., Goto Y., Shimizu F., Wakebe H., Hishigaki H., Watanabe T., Sugiyama A., Takemoto M., Kawakami B., Yamazaki M., Watanabe K., Kumagai A., Itakura S., Fukuzumi Y., Fujimori Y., Komiyama M., Tashiro H., Tanigami A., Fujiwara T., Ono T., Yamada K., Fujii Y., Ozaki K., Hirao M., Ohmori Y., Kawabata A., Hikiji T., Kobatake N., Inagaki H., Ikema Y., Okamoto S., Okitani R., Kawakami T., Noguchi S., Itoh T., Shigeta K., Senba T., Matsumura K., Nakajima Y., Mizuno T., Morinaga M., Sasaki M., Togashi T., Oyama M., Hata H., Watanabe M., Komatsu T., Mizushima-Sugano J., Satoh T., Shirai Y., Takahashi Y., Nakagawa K., Okumura K., Nagase T., Nomura N., Kikuchi H., Masuho Y., Yamashita R., Nakai K., Yada T., Nakamura Y., Ohara O., Isogai T., Sugano S.
    Nat. Genet. 36:40-45(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORMS 1; 2 AND 3).
    Tissue: Brain cortex, Embryo and Thalamus.
  2. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
    Tissue: Endometrium.
  3. "Human chromosome 7: DNA sequence and biology."
    Scherer S.W., Cheung J., MacDonald J.R., Osborne L.R., Nakabayashi K., Herbrick J.-A., Carson A.R., Parker-Katiraee L., Skaug J., Khaja R., Zhang J., Hudek A.K., Li M., Haddad M., Duggan G.E., Fernandez B.A., Kanematsu E., Gentles S.
    , Christopoulos C.C., Choufani S., Kwasnicka D., Zheng X.H., Lai Z., Nusskern D.R., Zhang Q., Gu Z., Lu F., Zeesman S., Nowaczyk M.J., Teshima I., Chitayat D., Shuman C., Weksberg R., Zackai E.H., Grebe T.A., Cox S.R., Kirkpatrick S.J., Rahman N., Friedman J.M., Heng H.H.Q., Pelicci P.G., Lo-Coco F., Belloni E., Shaffer L.G., Pober B., Morton C.C., Gusella J.F., Bruns G.A.P., Korf B.R., Quade B.J., Ligon A.H., Ferguson H., Higgins A.W., Leach N.T., Herrick S.R., Lemyre E., Farra C.G., Kim H.-G., Summers A.M., Gripp K.W., Roberts W., Szatmari P., Winsor E.J.T., Grzeschik K.-H., Teebi A., Minassian B.A., Kere J., Armengol L., Pujana M.A., Estivill X., Wilson M.D., Koop B.F., Tosi S., Moore G.E., Boright A.P., Zlotorynski E., Kerem B., Kroisel P.M., Petek E., Oscier D.G., Mould S.J., Doehner H., Doehner K., Rommens J.M., Vincent J.B., Venter J.C., Li P.W., Mural R.J., Adams M.D., Tsui L.-C.
    Science 300:767-772(2003) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
  4. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
  5. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
    Tissue: Brain and Pancreas.
  6. "Identification of human transforming gene."
    Kim J.W.
    Submitted (DEC-2002) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 6-227 (ISOFORM 3).
  7. "Large-scale identification and characterization of human genes that activate NF-kappaB and MAPK signaling pathways."
    Matsuda A., Suzuki Y., Honda G., Muramatsu S., Matsuzaki O., Nagano Y., Doi T., Shimotohno K., Harada T., Nishida E., Hayashi H., Sugano S.
    Oncogene 22:3307-3318(2003) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 119-227 (ISOFORMS 1/2).
    Tissue: Lung fibroblast.
  8. "Novel oxidative stress-responsive gene ERS25 functions as a regulator of the heat-shock and cell death response."
    Hwang S.O., Boswell S.A., Seo J.-S., Lee S.W.
    J. Biol. Chem. 283:13063-13069(2008) [PubMed] [Europe PMC] [Abstract]
    Cited for: IDENTIFICATION.
  9. "Glycoproteomics analysis of human liver tissue by combination of multiple enzyme digestion and hydrazide chemistry."
    Chen R., Jiang X., Sun D., Han G., Wang F., Ye M., Wang L., Zou H.
    J. Proteome Res. 8:651-661(2009) [PubMed] [Europe PMC] [Abstract]
    Cited for: GLYCOSYLATION [LARGE SCALE ANALYSIS] AT ASN-117.
    Tissue: Liver.
  10. Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
  11. Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].

Entry informationi

Entry nameiTMED4_HUMAN
AccessioniPrimary (citable) accession number: Q7Z7H5
Secondary accession number(s): A4D2K8
, B4DFJ4, Q56VW3, Q7Z432, Q8N2P6
Entry historyi
Integrated into UniProtKB/Swiss-Prot: March 29, 2005
Last sequence update: October 1, 2003
Last modified: June 8, 2016
This is version 113 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Human chromosome 7
    Human chromosome 7: entries, gene names and cross-references to MIM
  2. MIM cross-references
    Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.