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Protein

Transmembrane emp24 domain-containing protein 4

Gene

TMED4

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Involved in vesicular protein trafficking, mainly in the early secretory pathway. targeting. Involved in the maintenance of the Golgi apparatus. Appears to play a role in the biosynthesis of secreted cargo including processing. Involved in endoplasmic reticulum stress response. May play a role in the regulation of heat-shock response and apoptosis (By similarity).By similarity

GO - Molecular functioni

  • signal transducer activity Source: UniProtKB

GO - Biological processi

  • positive regulation of I-kappaB kinase/NF-kappaB signaling Source: UniProtKB
  • protein transport Source: UniProtKB-KW
Complete GO annotation...

Keywords - Biological processi

Protein transport, Transport

Names & Taxonomyi

Protein namesi
Recommended name:
Transmembrane emp24 domain-containing protein 4
Alternative name(s):
Endoplasmic reticulum stress-response protein 25
Short name:
ERS25
GMP25iso
Putative NF-kappa-B-activating protein 156
p24 family protein alpha-3
Short name:
p24alpha3
Gene namesi
Name:TMED4
Synonyms:ERS25
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
Proteomesi
  • UP000005640 Componenti: Chromosome 7

Organism-specific databases

HGNCiHGNC:22301. TMED4.

Subcellular locationi

Topology

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Topological domaini30 – 194LumenalSequence analysisAdd BLAST165
Transmembranei195 – 212HelicalSequence analysisAdd BLAST18
Topological domaini213 – 227CytoplasmicSequence analysisAdd BLAST15

GO - Cellular componenti

  • endoplasmic reticulum membrane Source: UniProtKB-SubCell
  • extracellular exosome Source: UniProtKB
  • integral component of membrane Source: UniProtKB-KW
Complete GO annotation...

Keywords - Cellular componenti

Endoplasmic reticulum, Membrane

Pathology & Biotechi

Organism-specific databases

DisGeNETi222068.
OpenTargetsiENSG00000158604.
PharmGKBiPA134983854.

Polymorphism and mutation databases

BioMutaiTMED4.
DMDMi62287892.

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Signal peptidei1 – 29Sequence analysisAdd BLAST29
ChainiPRO_000001038730 – 227Transmembrane emp24 domain-containing protein 4Add BLAST198

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Glycosylationi117N-linked (GlcNAc...)1 Publication1

Keywords - PTMi

Glycoprotein

Proteomic databases

EPDiQ7Z7H5.
MaxQBiQ7Z7H5.
PaxDbiQ7Z7H5.
PeptideAtlasiQ7Z7H5.
PRIDEiQ7Z7H5.

PTM databases

iPTMnetiQ7Z7H5.
PhosphoSitePlusiQ7Z7H5.
SwissPalmiQ7Z7H5.

Expressioni

Gene expression databases

BgeeiENSG00000158604.
CleanExiHS_TMED4.
ExpressionAtlasiQ7Z7H5. baseline and differential.
GenevisibleiQ7Z7H5. HS.

Organism-specific databases

HPAiHPA014650.

Interactioni

Protein-protein interaction databases

BioGridi128780. 29 interactors.
IntActiQ7Z7H5. 6 interactors.
STRINGi9606.ENSP00000404042.

Structurei

3D structure databases

ProteinModelPortaliQ7Z7H5.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini39 – 137GOLDPROSITE-ProRule annotationAdd BLAST99

Coiled coil

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Coiled coili147 – 176Sequence analysisAdd BLAST30

Motif

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Motifi220 – 227COPI vesicle coat-bindingSequence analysis8
Motifi220 – 221COPII vesicle coat-bindingSequence analysis2

Sequence similaritiesi

Belongs to the EMP24/GP25L family.Curated
Contains 1 GOLD domain.PROSITE-ProRule annotation

Keywords - Domaini

Coiled coil, Signal, Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiKOG1690. Eukaryota.
ENOG410XPUW. LUCA.
GeneTreeiENSGT00430000030848.
HOGENOMiHOG000160228.
HOVERGENiHBG105357.
InParanoidiQ7Z7H5.
KOiK20346.
OMAiLGMHVEI.
OrthoDBiEOG091G0JDW.
PhylomeDBiQ7Z7H5.
TreeFamiTF314123.

Family and domain databases

InterProiIPR009038. GOLD_dom.
IPR015720. TMP21-related.
[Graphical view]
PANTHERiPTHR22811. PTHR22811. 1 hit.
PfamiPF01105. EMP24_GP25L. 1 hit.
[Graphical view]
SMARTiSM01190. EMP24_GP25L. 1 hit.
[Graphical view]
PROSITEiPS50866. GOLD. 1 hit.
[Graphical view]

Sequences (3)i

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

This entry describes 3 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 1 (identifier: Q7Z7H5-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MAGVGAGPLR AMGRQALLLL ALCATGAQGL YFHIGETEKR CFIEEIPDET
60 70 80 90 100
MVIGNYRTQM WDKQKEVFLP STPGLGMHVE VKDPDGKVVL SRQYGSEGRF
110 120 130 140 150
TFTSHTPGDH QICLHSNSTR MALFAGGKLR VHLDIQVGEH ANNYPEIAAK
160 170 180 190 200
DKLTELQLRA RQLLDQVEQI QKEQDYQRYR EERFRLTSES TNQRVLWWSI
210 220
AQTVILILTG IWQMRHLKSF FEAKKLV
Length:227
Mass (Da):25,943
Last modified:October 1, 2003 - v1
Checksum:i3C650402F472A051
GO
Isoform 2 (identifier: Q7Z7H5-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     59-74: Missing.

Note: No experimental confirmation available.
Show »
Length:211
Mass (Da):24,070
Checksum:iD8111386449A8A53
GO
Isoform 3 (identifier: Q7Z7H5-3) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     179-186: YREERFRL → ASAYLLVI
     187-227: Missing.

Show »
Length:186
Mass (Da):20,781
Checksum:iDCEE9F5107DC4347
GO

Sequence cautioni

The sequence AAP20105 differs from that shown. Reason: Erroneous initiation. Translation N-terminally extended.Curated
The sequence BAC77362 differs from that shown. Reason: Erroneous initiation. Translation N-terminally extended.Curated

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_03569859 – 74Missing in isoform 2. 1 PublicationAdd BLAST16
Alternative sequenceiVSP_035699179 – 186YREERFRL → ASAYLLVI in isoform 3. 2 Publications8
Alternative sequenceiVSP_035700187 – 227Missing in isoform 3. 2 PublicationsAdd BLAST41

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AK074557 mRNA. Translation: BAC11058.1.
AK290125 mRNA. Translation: BAF82814.1.
AK294122 mRNA. Translation: BAG57455.1.
BX647965 mRNA. No translation available.
CH236960 Genomic DNA. Translation: EAL23751.1.
CH471128 Genomic DNA. Translation: EAW61090.1.
CH471128 Genomic DNA. Translation: EAW61091.1.
BC052641 mRNA. Translation: AAH52641.1.
BC057851 mRNA. Translation: AAH57851.1.
AY191223 mRNA. Translation: AAP20105.1. Different initiation.
AB097009 mRNA. Translation: BAC77362.1. Different initiation.
CCDSiCCDS5493.1. [Q7Z7H5-1]
CCDS78227.1. [Q7Z7H5-3]
RefSeqiNP_001289987.1. NM_001303058.1. [Q7Z7H5-3]
NP_001289989.1. NM_001303060.1.
NP_872353.2. NM_182547.3. [Q7Z7H5-1]
UniGeneiHs.598832.

Genome annotation databases

EnsembliENST00000457408; ENSP00000404042; ENSG00000158604. [Q7Z7H5-1]
ENST00000481238; ENSP00000417443; ENSG00000158604. [Q7Z7H5-3]
GeneIDi222068.
KEGGihsa:222068.
UCSCiuc003tli.4. human. [Q7Z7H5-1]

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AK074557 mRNA. Translation: BAC11058.1.
AK290125 mRNA. Translation: BAF82814.1.
AK294122 mRNA. Translation: BAG57455.1.
BX647965 mRNA. No translation available.
CH236960 Genomic DNA. Translation: EAL23751.1.
CH471128 Genomic DNA. Translation: EAW61090.1.
CH471128 Genomic DNA. Translation: EAW61091.1.
BC052641 mRNA. Translation: AAH52641.1.
BC057851 mRNA. Translation: AAH57851.1.
AY191223 mRNA. Translation: AAP20105.1. Different initiation.
AB097009 mRNA. Translation: BAC77362.1. Different initiation.
CCDSiCCDS5493.1. [Q7Z7H5-1]
CCDS78227.1. [Q7Z7H5-3]
RefSeqiNP_001289987.1. NM_001303058.1. [Q7Z7H5-3]
NP_001289989.1. NM_001303060.1.
NP_872353.2. NM_182547.3. [Q7Z7H5-1]
UniGeneiHs.598832.

3D structure databases

ProteinModelPortaliQ7Z7H5.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi128780. 29 interactors.
IntActiQ7Z7H5. 6 interactors.
STRINGi9606.ENSP00000404042.

PTM databases

iPTMnetiQ7Z7H5.
PhosphoSitePlusiQ7Z7H5.
SwissPalmiQ7Z7H5.

Polymorphism and mutation databases

BioMutaiTMED4.
DMDMi62287892.

Proteomic databases

EPDiQ7Z7H5.
MaxQBiQ7Z7H5.
PaxDbiQ7Z7H5.
PeptideAtlasiQ7Z7H5.
PRIDEiQ7Z7H5.

Protocols and materials databases

DNASUi222068.
Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENST00000457408; ENSP00000404042; ENSG00000158604. [Q7Z7H5-1]
ENST00000481238; ENSP00000417443; ENSG00000158604. [Q7Z7H5-3]
GeneIDi222068.
KEGGihsa:222068.
UCSCiuc003tli.4. human. [Q7Z7H5-1]

Organism-specific databases

CTDi222068.
DisGeNETi222068.
GeneCardsiTMED4.
HGNCiHGNC:22301. TMED4.
HPAiHPA014650.
MIMi612038. gene.
neXtProtiNX_Q7Z7H5.
OpenTargetsiENSG00000158604.
PharmGKBiPA134983854.
GenAtlasiSearch...

Phylogenomic databases

eggNOGiKOG1690. Eukaryota.
ENOG410XPUW. LUCA.
GeneTreeiENSGT00430000030848.
HOGENOMiHOG000160228.
HOVERGENiHBG105357.
InParanoidiQ7Z7H5.
KOiK20346.
OMAiLGMHVEI.
OrthoDBiEOG091G0JDW.
PhylomeDBiQ7Z7H5.
TreeFamiTF314123.

Miscellaneous databases

ChiTaRSiTMED4. human.
GeneWikiiTMED4.
GenomeRNAii222068.
PROiQ7Z7H5.
SOURCEiSearch...

Gene expression databases

BgeeiENSG00000158604.
CleanExiHS_TMED4.
ExpressionAtlasiQ7Z7H5. baseline and differential.
GenevisibleiQ7Z7H5. HS.

Family and domain databases

InterProiIPR009038. GOLD_dom.
IPR015720. TMP21-related.
[Graphical view]
PANTHERiPTHR22811. PTHR22811. 1 hit.
PfamiPF01105. EMP24_GP25L. 1 hit.
[Graphical view]
SMARTiSM01190. EMP24_GP25L. 1 hit.
[Graphical view]
PROSITEiPS50866. GOLD. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiTMED4_HUMAN
AccessioniPrimary (citable) accession number: Q7Z7H5
Secondary accession number(s): A4D2K8
, B4DFJ4, Q56VW3, Q7Z432, Q8N2P6
Entry historyi
Integrated into UniProtKB/Swiss-Prot: March 29, 2005
Last sequence update: October 1, 2003
Last modified: November 30, 2016
This is version 118 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Human chromosome 7
    Human chromosome 7: entries, gene names and cross-references to MIM
  2. MIM cross-references
    Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.