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Protein

Ubiquitin-conjugating enzyme E2 Q1

Gene

UBE2Q1

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Catalyzes the covalent attachment of ubiquitin to other proteins (Potential). May be involved in hormonal homeostasis in females. Involved in regulation of B4GALT1 cell surface expression, B4GALT1-mediated cell adhesion to laminin and embryoid body formation.PROSITE-ProRule annotationBy similarity

Catalytic activityi

S-ubiquitinyl-[E1 ubiquitin-activating enzyme]-L-cysteine + [E2 ubiquitin-conjugating enzyme]-L-cysteine = [E1 ubiquitin-activating enzyme]-L-cysteine + S-ubiquitinyl-[E2 ubiquitin-conjugating enzyme]-L-cysteine.PROSITE-ProRule annotation

Pathwayi: protein ubiquitination

This protein is involved in the pathway protein ubiquitination, which is part of Protein modification.PROSITE-ProRule annotation
View all proteins of this organism that are known to be involved in the pathway protein ubiquitination and in Protein modification.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei351 – 3511Glycyl thioester intermediatePROSITE-ProRule annotation

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Transferase

Keywords - Biological processi

Ubl conjugation pathway

Keywords - Ligandi

ATP-binding, Nucleotide-binding

Enzyme and pathway databases

ReactomeiR-HSA-983168. Antigen processing: Ubiquitination & Proteasome degradation.
SignaLinkiQ7Z7E8.
UniPathwayiUPA00143.

Names & Taxonomyi

Protein namesi
Recommended name:
Ubiquitin-conjugating enzyme E2 Q1 (EC:2.3.2.23)
Alternative name(s):
E2 ubiquitin-conjugating enzyme Q1
Protein NICE-5
Ubiquitin carrier protein Q1
Ubiquitin-protein ligase Q1
Gene namesi
Name:UBE2Q1
Synonyms:NICE5, UBE2Q
ORF Names:PRO3094
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
Proteomesi
  • UP000005640 Componenti: Chromosome 1

Organism-specific databases

HGNCiHGNC:15698. UBE2Q1.

Subcellular locationi

  • Nucleus By similarity
  • Cell projectionfilopodium By similarity
  • Cytoplasmcytosol By similarity

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cell projection, Cytoplasm, Nucleus

Pathology & Biotechi

Organism-specific databases

PharmGKBiPA38029.

Polymorphism and mutation databases

BioMutaiUBE2Q1.
DMDMi74750234.

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 422422Ubiquitin-conjugating enzyme E2 Q1PRO_0000223876Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei1 – 11N-acetylmethionineCombined sources

Keywords - PTMi

Acetylation

Proteomic databases

EPDiQ7Z7E8.
MaxQBiQ7Z7E8.
PaxDbiQ7Z7E8.
PeptideAtlasiQ7Z7E8.
PRIDEiQ7Z7E8.

PTM databases

iPTMnetiQ7Z7E8.
PhosphoSiteiQ7Z7E8.

Expressioni

Tissue specificityi

Widely expressed.1 Publication

Gene expression databases

BgeeiENSG00000160714.
CleanExiHS_UBE2Q1.
GenevisibleiQ7Z7E8. HS.

Organism-specific databases

HPAiHPA063368.

Interactioni

Subunit structurei

Monomer and homodimer. Only the homodimer is linked to ubiquitin through thiolester activation. Interacts (via N-terminus) with B4GALT1 (via N-terminal cytoplasmic domain). The interaction is direct.By similarity

Binary interactionsi

WithEntry#Exp.IntActNotes
FHL3Q136433EBI-10258181,EBI-741101
STUB1Q9UNE73EBI-1783287,EBI-357085

Protein-protein interaction databases

BioGridi120732. 18 interactions.
IntActiQ7Z7E8. 8 interactions.
MINTiMINT-7944200.
STRINGi9606.ENSP00000292211.

Structurei

Secondary structure

1
422
Legend: HelixTurnBeta strand
Show more details
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Helixi250 – 26415Combined sources
Helixi267 – 2704Combined sources
Beta strandi273 – 2786Combined sources
Helixi279 – 2813Combined sources
Beta strandi285 – 2917Combined sources
Helixi298 – 30912Combined sources
Beta strandi315 – 3206Combined sources
Turni325 – 3273Combined sources
Beta strandi331 – 3377Combined sources
Beta strandi339 – 3413Combined sources
Beta strandi348 – 3503Combined sources
Turni357 – 3593Combined sources
Helixi366 – 37914Combined sources
Beta strandi386 – 3883Combined sources
Helixi390 – 3923Combined sources
Helixi395 – 40511Combined sources

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
2QGXX-ray2.56A/B/C/D247-414[»]
ProteinModelPortaliQ7Z7E8.
SMRiQ7Z7E8. Positions 249-409.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiQ7Z7E8.

Family & Domainsi

Compositional bias

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Compositional biasi8 – 3831Gly-richAdd
BLAST

Sequence similaritiesi

Belongs to the ubiquitin-conjugating enzyme family.PROSITE-ProRule annotation

Phylogenomic databases

eggNOGiKOG0897. Eukaryota.
ENOG410XRET. LUCA.
GeneTreeiENSGT00390000018573.
HOVERGENiHBG061368.
InParanoidiQ7Z7E8.
KOiK10582.
OMAiSSAYTME.
OrthoDBiEOG091G0LGG.
PhylomeDBiQ7Z7E8.
TreeFamiTF313338.

Family and domain databases

Gene3Di3.10.110.10. 1 hit.
InterProiIPR000608. UBQ-conjugat_E2.
IPR016135. UBQ-conjugating_enzyme/RWD.
[Graphical view]
PfamiPF00179. UQ_con. 1 hit.
[Graphical view]
SUPFAMiSSF54495. SSF54495. 2 hits.
PROSITEiPS50127. UBIQUITIN_CONJUGAT_2. 1 hit.
[Graphical view]

Sequences (2)i

Sequence statusi: Complete.

This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 1 (identifier: Q7Z7E8-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MQQPQPQGQQ QPGPGQQLGG QGAAPGAGGG PGGGPGPGPC LRRELKLLES
60 70 80 90 100
IFHRGHERFR IASACLDELS CEFLLAGAGG AGAGAAPGPH LPPRGSVPGD
110 120 130 140 150
PVRIHCNITE SYPAVPPIWS VESDDPNLAA VLERLVDIKK GNTLLLQHLK
160 170 180 190 200
RIISDLCKLY NLPQHPDVEM LDQPLPAEQC TQEDVSSEDE DEEMPEDTED
210 220 230 240 250
LDHYEMKEEE PAEGKKSEDD GIGKENLAIL EKIKKNQRQD YLNGAVSGSV
260 270 280 290 300
QATDRLMKEL RDIYRSQSFK GGNYAVELVN DSLYDWNVKL LKVDQDSALH
310 320 330 340 350
NDLQILKEKE GADFILLNFS FKDNFPFDPP FVRVVSPVLS GGYVLGGGAI
360 370 380 390 400
CMELLTKQGW SSAYSIESVI MQISATLVKG KARVQFGANK SQYSLTRAQQ
410 420
SYKSLVQIHE KNGWYTPPKE DG
Length:422
Mass (Da):46,127
Last modified:October 1, 2003 - v1
Checksum:i106FF7E59DF65555
GO
Isoform 2 (identifier: Q7Z7E8-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     1-169: Missing.

Show »
Length:253
Mass (Da):28,473
Checksum:i63C896449FD35EF2
GO

Sequence cautioni

The sequence AAF71141 differs from that shown. Reason: Erroneous initiation. Curated
The sequence AAH61583 differs from that shown. Reason: Erroneous initiation. Curated
The sequence AAH87836 differs from that shown. Reason: Erroneous initiation. Curated
The sequence AAI07058 differs from that shown. Reason: Erroneous initiation. Curated
The sequence BAB71173 differs from that shown. Reason: Erroneous initiation. Curated
The sequence CAB65097 differs from that shown. Reason: Erroneous initiation. Curated

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti287 – 2871N → Y in CAB65097 (PubMed:11230159).Curated

Alternative sequence

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Alternative sequencei1 – 169169Missing in isoform 2. 2 PublicationsVSP_017296Add
BLAST

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AY112698 mRNA. Translation: AAM60814.1.
AY948200 mRNA. Translation: AAY23342.1.
AK056388 mRNA. Translation: BAB71173.1. Different initiation.
AK293986 mRNA. Translation: BAG57353.1.
AL592078 Genomic DNA. Translation: CAI16186.1.
CH471121 Genomic DNA. Translation: EAW53193.1.
BC000848 mRNA. Translation: AAH00848.1.
BC015316 mRNA. Translation: AAH15316.2.
BC061583 mRNA. Translation: AAH61583.1. Different initiation.
BC070158 mRNA. Translation: AAH70158.1.
BC087836 mRNA. Translation: AAH87836.1. Different initiation.
BC107057 mRNA. Translation: AAI07058.1. Different initiation.
AJ243666 mRNA. Translation: CAB65097.1. Different initiation.
AF116721 mRNA. Translation: AAF71141.1. Different initiation.
CR457219 mRNA. Translation: CAG33500.1.
CCDSiCCDS1069.1. [Q7Z7E8-1]
RefSeqiNP_060052.3. NM_017582.6. [Q7Z7E8-1]
UniGeneiHs.607928.

Genome annotation databases

EnsembliENST00000292211; ENSP00000292211; ENSG00000160714. [Q7Z7E8-1]
GeneIDi55585.
KEGGihsa:55585.
UCSCiuc001fff.2. human. [Q7Z7E8-1]

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AY112698 mRNA. Translation: AAM60814.1.
AY948200 mRNA. Translation: AAY23342.1.
AK056388 mRNA. Translation: BAB71173.1. Different initiation.
AK293986 mRNA. Translation: BAG57353.1.
AL592078 Genomic DNA. Translation: CAI16186.1.
CH471121 Genomic DNA. Translation: EAW53193.1.
BC000848 mRNA. Translation: AAH00848.1.
BC015316 mRNA. Translation: AAH15316.2.
BC061583 mRNA. Translation: AAH61583.1. Different initiation.
BC070158 mRNA. Translation: AAH70158.1.
BC087836 mRNA. Translation: AAH87836.1. Different initiation.
BC107057 mRNA. Translation: AAI07058.1. Different initiation.
AJ243666 mRNA. Translation: CAB65097.1. Different initiation.
AF116721 mRNA. Translation: AAF71141.1. Different initiation.
CR457219 mRNA. Translation: CAG33500.1.
CCDSiCCDS1069.1. [Q7Z7E8-1]
RefSeqiNP_060052.3. NM_017582.6. [Q7Z7E8-1]
UniGeneiHs.607928.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
2QGXX-ray2.56A/B/C/D247-414[»]
ProteinModelPortaliQ7Z7E8.
SMRiQ7Z7E8. Positions 249-409.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi120732. 18 interactions.
IntActiQ7Z7E8. 8 interactions.
MINTiMINT-7944200.
STRINGi9606.ENSP00000292211.

PTM databases

iPTMnetiQ7Z7E8.
PhosphoSiteiQ7Z7E8.

Polymorphism and mutation databases

BioMutaiUBE2Q1.
DMDMi74750234.

Proteomic databases

EPDiQ7Z7E8.
MaxQBiQ7Z7E8.
PaxDbiQ7Z7E8.
PeptideAtlasiQ7Z7E8.
PRIDEiQ7Z7E8.

Protocols and materials databases

DNASUi55585.
Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENST00000292211; ENSP00000292211; ENSG00000160714. [Q7Z7E8-1]
GeneIDi55585.
KEGGihsa:55585.
UCSCiuc001fff.2. human. [Q7Z7E8-1]

Organism-specific databases

CTDi55585.
GeneCardsiUBE2Q1.
H-InvDBHIX0001106.
HGNCiHGNC:15698. UBE2Q1.
HPAiHPA063368.
neXtProtiNX_Q7Z7E8.
PharmGKBiPA38029.
GenAtlasiSearch...

Phylogenomic databases

eggNOGiKOG0897. Eukaryota.
ENOG410XRET. LUCA.
GeneTreeiENSGT00390000018573.
HOVERGENiHBG061368.
InParanoidiQ7Z7E8.
KOiK10582.
OMAiSSAYTME.
OrthoDBiEOG091G0LGG.
PhylomeDBiQ7Z7E8.
TreeFamiTF313338.

Enzyme and pathway databases

UniPathwayiUPA00143.
ReactomeiR-HSA-983168. Antigen processing: Ubiquitination & Proteasome degradation.
SignaLinkiQ7Z7E8.

Miscellaneous databases

ChiTaRSiUBE2Q1. human.
EvolutionaryTraceiQ7Z7E8.
GenomeRNAii55585.
PROiQ7Z7E8.

Gene expression databases

BgeeiENSG00000160714.
CleanExiHS_UBE2Q1.
GenevisibleiQ7Z7E8. HS.

Family and domain databases

Gene3Di3.10.110.10. 1 hit.
InterProiIPR000608. UBQ-conjugat_E2.
IPR016135. UBQ-conjugating_enzyme/RWD.
[Graphical view]
PfamiPF00179. UQ_con. 1 hit.
[Graphical view]
SUPFAMiSSF54495. SSF54495. 2 hits.
PROSITEiPS50127. UBIQUITIN_CONJUGAT_2. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiUB2Q1_HUMAN
AccessioniPrimary (citable) accession number: Q7Z7E8
Secondary accession number(s): B4DF92
, Q29SN7, Q3B841, Q5I0X2, Q6IS04, Q6P7P2, Q96MV4, Q9BVX5, Q9UGL6
Entry historyi
Integrated into UniProtKB/Swiss-Prot: February 21, 2006
Last sequence update: October 1, 2003
Last modified: September 7, 2016
This is version 125 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. Human chromosome 1
    Human chromosome 1: entries, gene names and cross-references to MIM
  2. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  3. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  4. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.