Q7Z7E8 (UB2Q1_HUMAN) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Ubiquitin-conjugating enzyme E2 Q1
EC=6.3.2.19

Alternative name(s):
Protein NICE-5
Ubiquitin carrier protein Q1
Ubiquitin-protein ligase Q1

Gene names

Name:	UBE2Q1
Synonyms:	NICE5, UBE2Q
ORF Names:	PRO3094

Organism

Homo sapiens (Human) [Reference proteome]

Taxonomic identifier

9606 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Deuterostomia › Chordata › Craniata › Vertebrata › Gnathostomata › Teleostomi › Euteleostomi › Sarcopterygii › Tetrapoda › Amniota › Mammalia › Theria › Eutheria › Euarchontoglires › Primates › Haplorrhini › Simiiformes › Catarrhini › Hominoidea › Hominidae › Homininae › Homo

Protein attributes

Sequence length	422 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Catalyzes the covalent attachment of ubiquitin to other proteins By similarity.
Catalytic activity	ATP + ubiquitin + protein lysine = AMP + diphosphate + protein N-ubiquityllysine.
Pathway	Protein modification; protein ubiquitination.
Tissue specificity	Widely expressed. Ref.6
Sequence similarities	Belongs to the ubiquitin-conjugating enzyme family.
Sequence caution	The sequence AAF71141.1 differs from that shown. Reason: Erroneous initiation. The sequence AAH61583.1 differs from that shown. Reason: Erroneous initiation. The sequence AAH87836.1 differs from that shown. Reason: Erroneous initiation. The sequence AAI07058.1 differs from that shown. Reason: Erroneous initiation. The sequence BAB71173.1 differs from that shown. Reason: Erroneous initiation. The sequence CAB65097.1 differs from that shown. Reason: Erroneous initiation.

Ontologies

Keywords
Biological process	Ubl conjugation pathway
Coding sequence diversity	Alternative splicing
Ligand	ATP-binding Nucleotide-binding
Molecular function	Ligase
Technical term	3D-structure Complete proteome Reference proteome
Gene Ontology (GO)
Molecular_function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW ubiquitin-protein ligase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Binary interactions

With	Entry	#Exp.	IntAct	Notes
STUB1	Q9UNE7	3	EBI-1783287,EBI-357085

Alternative products

This entry describes 2 isoforms produced by alternative splicing. [Align] [Select]

Isoform 1 (identifier: Q7Z7E8-1)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

Isoform 2 (identifier: Q7Z7E8-2)

The sequence of this isoform differs from the canonical sequence as follows:
     1-169: Missing.

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Chain

1 – 422

422

Ubiquitin-conjugating enzyme E2 Q1

PRO_0000223876

Regions

Compositional bias

8 – 38

31

Gly-rich

Sites

Active site

351

1

Glycyl thioester intermediate By similarity

Natural variations

Alternative sequence

1 – 169

169

Missing in isoform 2.

VSP_017296

Experimental info

Sequence conflict

287

1

N → Y Ref.6

Secondary structure

1

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422

Helix Strand Turn

Details...

Helix

250 – 264

15

Helix

267 – 270

4

Beta strand

273 – 278

6

Helix

279 – 281

3

Beta strand

285 – 291

7

Helix

298 – 309

12

Beta strand

315 – 320

6

Turn

325 – 327

3

Beta strand

331 – 337

7

Beta strand

339 – 341

3

Beta strand

348 – 350

3

Turn

357 – 359

3

Helix

366 – 379

14

Beta strand

386 – 388

3

Helix

390 – 392

3

Helix

395 – 405

11

Sequences

	Sequence		Length	Mass (Da)
	Isoform 1 [UniParc]. Last modified October 1, 2003. Version 1. Checksum: 106FF7E59DF65555	FASTA	422	46,127
	10 20 30 40 50 60 MQQPQPQGQQ QPGPGQQLGG QGAAPGAGGG PGGGPGPGPC LRRELKLLES IFHRGHERFR 70 80 90 100 110 120 IASACLDELS CEFLLAGAGG AGAGAAPGPH LPPRGSVPGD PVRIHCNITE SYPAVPPIWS 130 140 150 160 170 180 VESDDPNLAA VLERLVDIKK GNTLLLQHLK RIISDLCKLY NLPQHPDVEM LDQPLPAEQC 190 200 210 220 230 240 TQEDVSSEDE DEEMPEDTED LDHYEMKEEE PAEGKKSEDD GIGKENLAIL EKIKKNQRQD 250 260 270 280 290 300 YLNGAVSGSV QATDRLMKEL RDIYRSQSFK GGNYAVELVN DSLYDWNVKL LKVDQDSALH 310 320 330 340 350 360 NDLQILKEKE GADFILLNFS FKDNFPFDPP FVRVVSPVLS GGYVLGGGAI CMELLTKQGW 370 380 390 400 410 420 SSAYSIESVI MQISATLVKG KARVQFGANK SQYSLTRAQQ SYKSLVQIHE KNGWYTPPKE DG
	Isoform 2 [UniParc]. Checksum: 63C896449FD35EF2	FASTA	253	28,473
	10 20 30 40 50 60 MLDQPLPAEQ CTQEDVSSED EDEEMPEDTE DLDHYEMKEE EPAEGKKSED DGIGKENLAI 70 80 90 100 110 120 LEKIKKNQRQ DYLNGAVSGS VQATDRLMKE LRDIYRSQSF KGGNYAVELV NDSLYDWNVK 130 140 150 160 170 180 LLKVDQDSAL HNDLQILKEK EGADFILLNF SFKDNFPFDP PFVRVVSPVL SGGYVLGGGA 190 200 210 220 230 240 ICMELLTKQG WSSAYSIESV IMQISATLVK GKARVQFGAN KSQYSLTRAQ QSYKSLVQIH 250 EKNGWYTPPK EDG

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Isolation and characterization of the human UBE2Q gene and its murine ortholog." Altmann M.E., Schulze E., Adham I.M., Koehler M., Engel W. Submitted (MAY-2002) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1).
[2]	"Complete sequencing and characterization of 21,243 full-length human cDNAs." Ota T., Suzuki Y., Nishikawa T., Otsuki T., Sugiyama T., Irie R., Wakamatsu A., Hayashi K., Sato H., Nagai K., Kimura K., Makita H., Sekine M., Obayashi M., Nishi T., Shibahara T., Tanaka T., Ishii S. , Yamamoto J., Saito K., Kawai Y., Isono Y., Nakamura Y., Nagahari K., Murakami K., Yasuda T., Iwayanagi T., Wagatsuma M., Shiratori A., Sudo H., Hosoiri T., Kaku Y., Kodaira H., Kondo H., Sugawara M., Takahashi M., Kanda K., Yokoi T., Furuya T., Kikkawa E., Omura Y., Abe K., Kamihara K., Katsuta N., Sato K., Tanikawa M., Yamazaki M., Ninomiya K., Ishibashi T., Yamashita H., Murakawa K., Fujimori K., Tanai H., Kimata M., Watanabe M., Hiraoka S., Chiba Y., Ishida S., Ono Y., Takiguchi S., Watanabe S., Yosida M., Hotuta T., Kusano J., Kanehori K., Takahashi-Fujii A., Hara H., Tanase T.-O., Nomura Y., Togiya S., Komai F., Hara R., Takeuchi K., Arita M., Imose N., Musashino K., Yuuki H., Oshima A., Sasaki N., Aotsuka S., Yoshikawa Y., Matsunawa H., Ichihara T., Shiohata N., Sano S., Moriya S., Momiyama H., Satoh N., Takami S., Terashima Y., Suzuki O., Nakagawa S., Senoh A., Mizoguchi H., Goto Y., Shimizu F., Wakebe H., Hishigaki H., Watanabe T., Sugiyama A., Takemoto M., Kawakami B., Yamazaki M., Watanabe K., Kumagai A., Itakura S., Fukuzumi Y., Fujimori Y., Komiyama M., Tashiro H., Tanigami A., Fujiwara T., Ono T., Yamada K., Fujii Y., Ozaki K., Hirao M., Ohmori Y., Kawabata A., Hikiji T., Kobatake N., Inagaki H., Ikema Y., Okamoto S., Okitani R., Kawakami T., Noguchi S., Itoh T., Shigeta K., Senba T., Matsumura K., Nakajima Y., Mizuno T., Morinaga M., Sasaki M., Togashi T., Oyama M., Hata H., Watanabe M., Komatsu T., Mizushima-Sugano J., Satoh T., Shirai Y., Takahashi Y., Nakagawa K., Okumura K., Nagase T., Nomura N., Kikuchi H., Masuho Y., Yamashita R., Nakai K., Yada T., Nakamura Y., Ohara O., Isogai T., Sugano S. Nat. Genet. 36:40-45(2004) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 2), NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 204-422. Tissue: Cerebellum.
[3]	"The DNA sequence and biological annotation of human chromosome 1." Gregory S.G., Barlow K.F., McLay K.E., Kaul R., Swarbreck D., Dunham A., Scott C.E., Howe K.L., Woodfine K., Spencer C.C.A., Jones M.C., Gillson C., Searle S., Zhou Y., Kokocinski F., McDonald L., Evans R., Phillips K. , Atkinson A., Cooper R., Jones C., Hall R.E., Andrews T.D., Lloyd C., Ainscough R., Almeida J.P., Ambrose K.D., Anderson F., Andrew R.W., Ashwell R.I.S., Aubin K., Babbage A.K., Bagguley C.L., Bailey J., Beasley H., Bethel G., Bird C.P., Bray-Allen S., Brown J.Y., Brown A.J., Buckley D., Burton J., Bye J., Carder C., Chapman J.C., Clark S.Y., Clarke G., Clee C., Cobley V., Collier R.E., Corby N., Coville G.J., Davies J., Deadman R., Dunn M., Earthrowl M., Ellington A.G., Errington H., Frankish A., Frankland J., French L., Garner P., Garnett J., Gay L., Ghori M.R.J., Gibson R., Gilby L.M., Gillett W., Glithero R.J., Grafham D.V., Griffiths C., Griffiths-Jones S., Grocock R., Hammond S., Harrison E.S.I., Hart E., Haugen E., Heath P.D., Holmes S., Holt K., Howden P.J., Hunt A.R., Hunt S.E., Hunter G., Isherwood J., James R., Johnson C., Johnson D., Joy A., Kay M., Kershaw J.K., Kibukawa M., Kimberley A.M., King A., Knights A.J., Lad H., Laird G., Lawlor S., Leongamornlert D.A., Lloyd D.M., Loveland J., Lovell J., Lush M.J., Lyne R., Martin S., Mashreghi-Mohammadi M., Matthews L., Matthews N.S.W., McLaren S., Milne S., Mistry S., Moore M.J.F., Nickerson T., O'Dell C.N., Oliver K., Palmeiri A., Palmer S.A., Parker A., Patel D., Pearce A.V., Peck A.I., Pelan S., Phelps K., Phillimore B.J., Plumb R., Rajan J., Raymond C., Rouse G., Saenphimmachak C., Sehra H.K., Sheridan E., Shownkeen R., Sims S., Skuce C.D., Smith M., Steward C., Subramanian S., Sycamore N., Tracey A., Tromans A., Van Helmond Z., Wall M., Wallis J.M., White S., Whitehead S.L., Wilkinson J.E., Willey D.L., Williams H., Wilming L., Wray P.W., Wu Z., Coulson A., Vaudin M., Sulston J.E., Durbin R.M., Hubbard T., Wooster R., Dunham I., Carter N.P., McVean G., Ross M.T., Harrow J., Olson M.V., Beck S., Rogers J., Bentley D.R. Nature 441:315-321(2006) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[4]	Mural R.J., Istrail S., Sutton G.G., Florea L., Halpern A.L., Mobarry C.M., Lippert R., Walenz B., Shatkay H., Dew I., Miller J.R., Flanigan M.J., Edwards N.J., Bolanos R., Fasulo D., Halldorsson B.V., Hannenhalli S., Turner R. , Yooseph S., Lu F., Nusskern D.R., Shue B.C., Zheng X.H., Zhong F., Delcher A.L., Huson D.H., Kravitz S.A., Mouchard L., Reinert K., Remington K.A., Clark A.G., Waterman M.S., Eichler E.E., Adams M.D., Hunkapiller M.W., Myers E.W., Venter J.C. Submitted (SEP-2005) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[5]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 2), NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 163-422 (ISOFORM 1). Tissue: Cervix and Uterus.
[6]	"Identification of human epidermal differentiation complex (EDC)-encoded genes by subtractive hybridization of entire YACs to a gridded keratinocyte cDNA library." Marenholz I., Zirra M., Fischer D.F., Backendorf C., Ziegler A., Mischke D. Genome Res. 11:341-355(2001) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 282-422, TISSUE SPECIFICITY. Tissue: Keratinocyte.
[7]	"Functional prediction of the coding sequences of 121 new genes deduced by analysis of cDNA clones from human fetal liver." Zhang C., Yu Y., Zhang S., Wei H., Zhou G., Ouyang S., Luo L., Bi J., Liu M., He F. Submitted (DEC-1998) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 312-386. Tissue: Fetal liver.
[8]	"Cloning of human full open reading frames in Gateway(TM) system entry vector (pDONR201)." Ebert L., Schick M., Neubert P., Schatten R., Henze S., Korn B. Submitted (JUN-2004) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 352-422.
[9]	"Initial characterization of the human central proteome." Burkard T.R., Planyavsky M., Kaupe I., Breitwieser F.P., Buerckstuemmer T., Bennett K.L., Superti-Furga G., Colinge J. BMC Syst. Biol. 5:17-17(2011) [PubMed] [Europe PMC] [Abstract] Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
+	Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ

AY112698 mRNA. Translation: AAM60814.1.
AK056388 mRNA. Translation: BAB71173.1. Different initiation.
AK293986 mRNA. Translation: BAG57353.1.
AL592078 Genomic DNA. Translation: CAI16186.1.
CH471121 Genomic DNA. Translation: EAW53193.1.
BC000848 mRNA. Translation: AAH00848.1.
BC015316 mRNA. Translation: AAH15316.2.
BC061583 mRNA. Translation: AAH61583.1. Different initiation.
BC070158 mRNA. Translation: AAH70158.1.
BC087836 mRNA. Translation: AAH87836.1. Different initiation.
BC107057 mRNA. Translation: AAI07058.1. Different initiation.
AJ243666 mRNA. Translation: CAB65097.1. Different initiation.
AF116721 mRNA. Translation: AAF71141.1. Different initiation.
CR457219 mRNA. Translation: CAG33500.1.

IPI

IPI00333410.
IPI00719397.

RefSeq

NP_060052.3. NM_017582.6.

UniGene

Hs.607928.

3D structure databases

PDBe
RCSB PDB
PDBj

Entry	Method	Resolution (Å)	Chain	Positions	PDBsum
2QGX	X-ray	2.56	A/B/C/D	247-414	[»]

ProteinModelPortal

Q7Z7E8.

ModBase

Search...

Protein-protein interaction databases

IntAct

Q7Z7E8. 5 interactions.

MINT

MINT-7944200.

PTM databases

PhosphoSite

Q7Z7E8.

Polymorphism databases

DMDM

74750234.

Proteomic databases

PaxDb

Q7Z7E8.

PRIDE

Q7Z7E8.

Protocols and materials databases

DNASU

55585.

StructuralBiologyKnowledgebase

Search...

Genome annotation databases

Ensembl

ENST00000292211; ENSP00000292211; ENSG00000160714.

GeneID

55585.

KEGG

hsa:55585.

UCSC

uc001fff.1. human.

Organism-specific databases

CTD

55585.

GeneCards

GC01M154521.

H-InvDB

HIX0001106.

HGNC

HGNC:15698. UBE2Q1.

neXtProt

NX_Q7Z7E8.

PharmGKB

PA38029.

GenAtlas

Search...

Phylogenomic databases

eggNOG

NOG320521.

HOVERGEN

HBG061368.

InParanoid

Q7Z7E8.

KO

K10582.

OMA

LYEWNIR.

OrthoDB

EOG48D0VK.

PhylomeDB

Q7Z7E8.

Enzyme and pathway databases

Reactome

REACT_6900. Immune System.

SignaLink

Q7Z7E8.

UniPathway

UPA00143.

Gene expression databases

ArrayExpress

Q7Z7E8.

Bgee

Q7Z7E8.

CleanEx

HS_UBE2Q1.

Genevestigator

Q7Z7E8.

GermOnline

ENSG00000160714. Homo sapiens.

Family and domain databases

Gene3D

3.10.110.10. 1 hit.

InterPro

IPR000608. UBQ-conjugat_E2.
IPR016135. UBQ-conjugating_enzyme/RWD.
[Graphical view]

Pfam

PF00179. UQ_con. 1 hit.
[Graphical view]

SUPFAM

SSF54495. UBQ-conjugat/RWD-like. 1 hit.

PROSITE

PS00183. UBIQUITIN_CONJUGAT_1. False negative.
PS50127. UBIQUITIN_CONJUGAT_2. 1 hit.
[Graphical view]

ProtoNet

Search...

Other

ChiTaRS

UBE2Q1. human.

EvolutionaryTrace

Q7Z7E8.

GenomeRNAi

55585.

NextBio

60100.

Entry information

Entry name

UB2Q1_HUMAN

Accession

Primary (citable) accession number: Q7Z7E8
Secondary accession number(s): B4DF92 , Q3B841, Q5I0X2, Q6IS04, Q6P7P2, Q96MV4, Q9BVX5, Q9UGL6

Entry history

Integrated into UniProtKB/Swiss-Prot:	February 21, 2006
Last sequence update:	October 1, 2003
Last modified:	May 29, 2013
This is version 94 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program

Disclaimer

Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.