Q7Z7C8 (TAF8_HUMAN) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Transcription initiation factor TFIID subunit 8

Alternative name(s):
Protein taube nuss
TBP-associated factor 43 kDa
TBP-associated factor 8
Transcription initiation factor TFIID 43 kDa subunit
Short name=TAFII-43
Short name=TAFII43
Short name=hTAFII43

Gene names

Name:	TAF8
Synonyms:	TAFII43, TBN

Organism

Homo sapiens (Human)

Taxonomic identifier

9606 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Primates › Haplorrhini › Catarrhini › Hominidae › Homo

Protein attributes

Sequence length	310 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Transcription factor TFIID is one of the general factors required for accurate and regulated initiation by RNA polymerase II. Mediates both basal and activator-dependent transcription. Plays a role in the differentiation of preadipocyte fibroblasts to adipocytes, however, does not seem to play a role in differentiation of myoblasts. Required for the integration of TAF10 in the TAF complex. May be important for survival of cells of the inner cell mass which constitute the pluripotent cell population of the early embryo By similarity. Ref.1
Subunit structure	TFIID is composed of TATA binding protein (TBP) and a number of TBP-associated factors (TAFs). Interacts with TBP, TAF1, TAF6, TAF10, TAF11 and TAF13. Component also of a small TAF complex (SMAT) containing TAF8, TAF10 and SUPT7L. Forms a heterodimer with TAF10. Interaction with TAF10 is mediated mainly via its histone fold domain while interaction with SUPT7L is via its C-terminal region. Ref.1 Ref.5 Ref.6
Subcellular location	Nucleus. Cytoplasm By similarity. Note: Localized in the cytoplasm and transported from the cytoplasm to the nucleus in some cells, possibly depending on the functional or developmental state of the cell By similarity. Ref.1
Induction	Induced with triglyceride accumulation.
Domain	Ectopic expression of the histone fold domain acts as a dominant-negative mutant resulting in differentiation inhibition.
Miscellaneous	'Taube nuss' means "empty nut' in German.
Sequence similarities	Belongs to the TAF8 family. Contains 1 histone-fold domain.
Sequence caution	The sequence BAB71460.1 differs from that shown. Reason: Frameshift at position 240.

Ontologies

Keywords
Biological process	Differentiation Transcription Transcription regulation
Cellular component	Cytoplasm Nucleus
Coding sequence diversity	Alternative splicing
Molecular function	Developmental protein
PTM	Acetylation Phosphoprotein
Technical term	Complete proteome Direct protein sequencing Reference proteome
Gene Ontology (GO)
Biological process	cell differentiation Inferred from electronic annotation. Source: UniProtKB-KW maintenance of protein location in nucleus Inferred from direct assay Ref.5. Source: HGNC positive regulation of transcription, DNA-dependent Inferred from direct assay Ref.1. Source: MGI regulation of fat cell differentiation Inferred from direct assay Ref.1. Source: MGI transcription, DNA-dependent Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	perinuclear region of cytoplasm Inferred from direct assay Ref.5. Source: HGNC transcription factor TFIID complex Inferred from direct assay Ref.1. Source: MGI
Molecular function	DNA binding Inferred from electronic annotation. Source: InterPro protein binding Inferred from physical interaction Ref.5. Source: HGNC
Complete GO annotation...

Alternative products

This entry describes 3 isoforms produced by alternative splicing. [Align] [Select]

Isoform 1 (identifier: Q7Z7C8-1)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

Isoform 2 (identifier: Q7Z7C8-2)

The sequence of this isoform differs from the canonical sequence as follows:
     308-310: SLS → PDTF

Isoform 3 (identifier: Q7Z7C8-4)

The sequence of this isoform differs from the canonical sequence as follows:
     261-310: EDSGAEKENT...PKIRRKKSLS → IESRSVTQAG...TSSITLICGR

Note: No experimental confirmation available.

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Initiator methionine	1	1	Removed
	Chain	2 – 310	309	Transcription initiation factor TFIID subunit 8		PRO_0000315396
Regions
	Domain	35 – 102	68	Histone-fold
	Motif	294 – 307	14	Nuclear localization signal Potential
Amino acid modifications
	Modified residue	2	1	N-acetylalanine Ref.8
	Modified residue	130	1	Phosphothreonine Ref.7
Natural variations
	Alternative sequence	261 – 310	50	EDSGA…KKSLS → IESRSVTQAGVQWQDLGSLQ PPPPGFKRFSSLSLLSSWNY RRILEPRRRTPLSCSRTPPC RVAGMGRRTSSITLICGR in isoform 3.		VSP_030548
	Alternative sequence	308 – 310	3	SLS → PDTF in isoform 2.		VSP_030549
Experimental info
	Sequence conflict	139	1	N → D in BAB71460. Ref.2

Sequences

	Sequence		Length	Mass (Da)
	Isoform 1 [UniParc]. Last modified October 1, 2003. Version 1. Checksum: 0F955828FE9FC0EA	FASTA	310	34,262
	10 20 30 40 50 60 MADAAATAGA GGSGTRSGSK QSTNPADNYH LARRRTLQVV VSSLLTEAGF ESAEKASVET 70 80 90 100 110 120 LTEMLQSYIS EIGRSAKSYC EHTARTQPTL SDIVVTLVEM GFNVDTLPAY AKRSQRMVIT 130 140 150 160 170 180 APPVTNQPVT PKALTAGQNR PHPPHIPSHF PEFPDPHTYI KTPTYREPVS DYQVLREKAA 190 200 210 220 230 240 SQRRDVERAL TRFMAKTGET QSLFKDDVST FPLIAARPFT IPYLTALLPS ELEMQQMEET 250 260 270 280 290 300 DSSEQDEQTD TENLALHISM EDSGAEKENT SVLQQNPSLS GSRNGEENII DNPYLRPVKK 310 PKIRRKKSLS
	Isoform 2 [UniParc]. Checksum: E54B88DAF8FE9FC0	FASTA	311	34,436
	10 20 30 40 50 60 MADAAATAGA GGSGTRSGSK QSTNPADNYH LARRRTLQVV VSSLLTEAGF ESAEKASVET 70 80 90 100 110 120 LTEMLQSYIS EIGRSAKSYC EHTARTQPTL SDIVVTLVEM GFNVDTLPAY AKRSQRMVIT 130 140 150 160 170 180 APPVTNQPVT PKALTAGQNR PHPPHIPSHF PEFPDPHTYI KTPTYREPVS DYQVLREKAA 190 200 210 220 230 240 SQRRDVERAL TRFMAKTGET QSLFKDDVST FPLIAARPFT IPYLTALLPS ELEMQQMEET 250 260 270 280 290 300 DSSEQDEQTD TENLALHISM EDSGAEKENT SVLQQNPSLS GSRNGEENII DNPYLRPVKK 310 PKIRRKKPDT F
	Isoform 3 [UniParc]. Checksum: B65123963AA96D63	FASTA	338	37,411
	10 20 30 40 50 60 MADAAATAGA GGSGTRSGSK QSTNPADNYH LARRRTLQVV VSSLLTEAGF ESAEKASVET 70 80 90 100 110 120 LTEMLQSYIS EIGRSAKSYC EHTARTQPTL SDIVVTLVEM GFNVDTLPAY AKRSQRMVIT 130 140 150 160 170 180 APPVTNQPVT PKALTAGQNR PHPPHIPSHF PEFPDPHTYI KTPTYREPVS DYQVLREKAA 190 200 210 220 230 240 SQRRDVERAL TRFMAKTGET QSLFKDDVST FPLIAARPFT IPYLTALLPS ELEMQQMEET 250 260 270 280 290 300 DSSEQDEQTD TENLALHISM IESRSVTQAG VQWQDLGSLQ PPPPGFKRFS SLSLLSSWNY 310 320 330 RRILEPRRRT PLSCSRTPPC RVAGMGRRTS SITLICGR

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"The TBN protein, which is essential for early embryonic mouse development, is an inducible TAFII implicated in adipogenesis." Guermah M., Ge K., Chiang C.-M., Roeder R.G. Mol. Cell 12:991-1001(2003) [PubMed: 14580349] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1), PROTEIN SEQUENCE OF 134-157; 162-178 AND 268-292, FUNCTION, SUBCELLULAR LOCATION, INTERACTION WITH TBP; TAF1; TAF6; TAF10 AND TAF11.
[2]	"Complete sequencing and characterization of 21,243 full-length human cDNAs." Ota T., Suzuki Y., Nishikawa T., Otsuki T., Sugiyama T., Irie R., Wakamatsu A., Hayashi K., Sato H., Nagai K., Kimura K., Makita H., Sekine M., Obayashi M., Nishi T., Shibahara T., Tanaka T., Ishii S. , Yamamoto J., Saito K., Kawai Y., Isono Y., Nakamura Y., Nagahari K., Murakami K., Yasuda T., Iwayanagi T., Wagatsuma M., Shiratori A., Sudo H., Hosoiri T., Kaku Y., Kodaira H., Kondo H., Sugawara M., Takahashi M., Kanda K., Yokoi T., Furuya T., Kikkawa E., Omura Y., Abe K., Kamihara K., Katsuta N., Sato K., Tanikawa M., Yamazaki M., Ninomiya K., Ishibashi T., Yamashita H., Murakawa K., Fujimori K., Tanai H., Kimata M., Watanabe M., Hiraoka S., Chiba Y., Ishida S., Ono Y., Takiguchi S., Watanabe S., Yosida M., Hotuta T., Kusano J., Kanehori K., Takahashi-Fujii A., Hara H., Tanase T.-O., Nomura Y., Togiya S., Komai F., Hara R., Takeuchi K., Arita M., Imose N., Musashino K., Yuuki H., Oshima A., Sasaki N., Aotsuka S., Yoshikawa Y., Matsunawa H., Ichihara T., Shiohata N., Sano S., Moriya S., Momiyama H., Satoh N., Takami S., Terashima Y., Suzuki O., Nakagawa S., Senoh A., Mizoguchi H., Goto Y., Shimizu F., Wakebe H., Hishigaki H., Watanabe T., Sugiyama A., Takemoto M., Kawakami B., Yamazaki M., Watanabe K., Kumagai A., Itakura S., Fukuzumi Y., Fujimori Y., Komiyama M., Tashiro H., Tanigami A., Fujiwara T., Ono T., Yamada K., Fujii Y., Ozaki K., Hirao M., Ohmori Y., Kawabata A., Hikiji T., Kobatake N., Inagaki H., Ikema Y., Okamoto S., Okitani R., Kawakami T., Noguchi S., Itoh T., Shigeta K., Senba T., Matsumura K., Nakajima Y., Mizuno T., Morinaga M., Sasaki M., Togashi T., Oyama M., Hata H., Watanabe M., Komatsu T., Mizushima-Sugano J., Satoh T., Shirai Y., Takahashi Y., Nakagawa K., Okumura K., Nagase T., Nomura N., Kikuchi H., Masuho Y., Yamashita R., Nakai K., Yada T., Nakamura Y., Ohara O., Isogai T., Sugano S. Nat. Genet. 36:40-45(2004) [PubMed: 14702039] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 3). Tissue: Testis.
[3]	"The DNA sequence and analysis of human chromosome 6." Mungall A.J., Palmer S.A., Sims S.K., Edwards C.A., Ashurst J.L., Wilming L., Jones M.C., Horton R., Hunt S.E., Scott C.E., Gilbert J.G.R., Clamp M.E., Bethel G., Milne S., Ainscough R., Almeida J.P., Ambrose K.D., Andrews T.D. , Ashwell R.I.S., Babbage A.K., Bagguley C.L., Bailey J., Banerjee R., Barker D.J., Barlow K.F., Bates K., Beare D.M., Beasley H., Beasley O., Bird C.P., Blakey S.E., Bray-Allen S., Brook J., Brown A.J., Brown J.Y., Burford D.C., Burrill W., Burton J., Carder C., Carter N.P., Chapman J.C., Clark S.Y., Clark G., Clee C.M., Clegg S., Cobley V., Collier R.E., Collins J.E., Colman L.K., Corby N.R., Coville G.J., Culley K.M., Dhami P., Davies J., Dunn M., Earthrowl M.E., Ellington A.E., Evans K.A., Faulkner L., Francis M.D., Frankish A., Frankland J., French L., Garner P., Garnett J., Ghori M.J., Gilby L.M., Gillson C.J., Glithero R.J., Grafham D.V., Grant M., Gribble S., Griffiths C., Griffiths M.N.D., Hall R., Halls K.S., Hammond S., Harley J.L., Hart E.A., Heath P.D., Heathcott R., Holmes S.J., Howden P.J., Howe K.L., Howell G.R., Huckle E., Humphray S.J., Humphries M.D., Hunt A.R., Johnson C.M., Joy A.A., Kay M., Keenan S.J., Kimberley A.M., King A., Laird G.K., Langford C., Lawlor S., Leongamornlert D.A., Leversha M., Lloyd C.R., Lloyd D.M., Loveland J.E., Lovell J., Martin S., Mashreghi-Mohammadi M., Maslen G.L., Matthews L., McCann O.T., McLaren S.J., McLay K., McMurray A., Moore M.J.F., Mullikin J.C., Niblett D., Nickerson T., Novik K.L., Oliver K., Overton-Larty E.K., Parker A., Patel R., Pearce A.V., Peck A.I., Phillimore B.J.C.T., Phillips S., Plumb R.W., Porter K.M., Ramsey Y., Ranby S.A., Rice C.M., Ross M.T., Searle S.M., Sehra H.K., Sheridan E., Skuce C.D., Smith S., Smith M., Spraggon L., Squares S.L., Steward C.A., Sycamore N., Tamlyn-Hall G., Tester J., Theaker A.J., Thomas D.W., Thorpe A., Tracey A., Tromans A., Tubby B., Wall M., Wallis J.M., West A.P., White S.S., Whitehead S.L., Whittaker H., Wild A., Willey D.J., Wilmer T.E., Wood J.M., Wray P.W., Wyatt J.C., Young L., Younger R.M., Bentley D.R., Coulson A., Durbin R.M., Hubbard T., Sulston J.E., Dunham I., Rogers J., Beck S. Nature 425:805-811(2003) [PubMed: 14574404] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[4]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed: 15489334] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 2).
[5]	"The nuclear import of TAF10 is regulated by one of its three histone fold domain-containing interaction partners." Soutoglou E., Demeny M.A., Scheer E., Fienga G., Sassone-Corsi P., Tora L. Mol. Cell. Biol. 25:4092-4104(2005) [PubMed: 15870280] [Abstract] Cited for: INTERACTION WITH TAF10.
[6]	"Identification of a small TAF complex and its role in the assembly of TAF-containing complexes." Demeny M.A., Soutoglou E., Nagy Z., Scheer E., Janoshazi A., Richardot M., Argentini M., Kessler P., Tora L. PLoS ONE 2:E316-E316(2007) [PubMed: 17375202] [Abstract] Cited for: INTERACTION IN A SMAT COMPLEX WITH TAF10 AND SUPT7L, INTERACTION IN A TFIID COMPLEX WITH TAF1; TAF6; TAF11 AND TBP.
[7]	"A quantitative atlas of mitotic phosphorylation." Dephoure N., Zhou C., Villen J., Beausoleil S.A., Bakalarski C.E., Elledge S.J., Gygi S.P. Proc. Natl. Acad. Sci. U.S.A. 105:10762-10767(2008) [PubMed: 18669648] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT THR-130, MASS SPECTROMETRY. Tissue: Cervix carcinoma.
[8]	"Lys-N and trypsin cover complementary parts of the phosphoproteome in a refined SCX-based approach." Gauci S., Helbig A.O., Slijper M., Krijgsveld J., Heck A.J., Mohammed S. Anal. Chem. 81:4493-4501(2009) [PubMed: 19413330] [Abstract] Cited for: ACETYLATION [LARGE SCALE ANALYSIS] AT ALA-2, MASS SPECTROMETRY. Tissue: Embryonic kidney.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AF465841 mRNA. Translation: AAP47094.1. AK057383 mRNA. Translation: BAB71460.1. Frameshift. AL513008, AL512274 Genomic DNA. Translation: CAI15202.1. AL513008, AL512274 Genomic DNA. Translation: CAI15203.1. AL512274, AL513008 Genomic DNA. Translation: CAI16781.1. AL512274, AL513008 Genomic DNA. Translation: CAI16782.1. BC119678 mRNA. Translation: AAI19679.1. BC119679 mRNA. Translation: AAI19680.1.
IPI	IPI00065313. IPI00878968. IPI00879798.
RefSeq	NP_612639.2. NM_138572.2.
UniGene	Hs.520122.
3D structure databases
ProteinModelPortal	Q7Z7C8.
SMR	Q7Z7C8. Positions 39-98.
ModBase	Search...
Protein-protein interaction databases
IntAct	Q7Z7C8. 1 interaction.
STRING	Q7Z7C8.
PTM databases
PhosphoSite	Q7Z7C8.
Polymorphism databases
DMDM	74723384.
Proteomic databases
PRIDE	Q7Z7C8.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
Ensembl	ENST00000372977; ENSP00000362068; ENSG00000137413.
GeneID	129685.
KEGG	hsa:129685.
UCSC	uc003orr.2. human. uc003ors.1. human. uc003ort.1. human. uc003orv.1. human.
Organism-specific databases
CTD	129685.
GeneCards	GC06P042018.
H-InvDB	HIX0022719.
HGNC	HGNC:17300. TAF8.
HPA	HPA031734.
MIM	609514. gene.
neXtProt	NX_Q7Z7C8.
PharmGKB	PA162405144.
GenAtlas	Search...
Phylogenomic databases
eggNOG	prNOG11149.
GeneTree	ENSGT00390000017567.
HOVERGEN	HBG105411.
OrthoDB	EOG4V6ZH6.
Gene expression databases
ArrayExpress	Q7Z7C8.
Bgee	Q7Z7C8.
CleanEx	HS_TAF8.
Genevestigator	Q7Z7C8.
Family and domain databases
InterPro	IPR006565. BTP. IPR009072. Histone-fold. IPR019473. TFIID_su8_C. [Graphical view]
Gene3D	G3DSA:1.10.20.10. Histone-fold. 1 hit.
KO	K14649.
Pfam	PF07524. Bromo_TP. 1 hit. PF10406. TAF8_C. 1 hit. [Graphical view]
SMART	SM00576. BTP. 1 hit. [Graphical view]
SUPFAM	SSF47113. Histone-fold. 1 hit.
ProtoNet	Search...
Other
NextBio	82629.
SOURCE	Search...

Entry information

Entry name

TAF8_HUMAN

Accession

Primary (citable) accession number: Q7Z7C8
Secondary accession number(s): Q5T0K1, Q8N4R9, Q96M52

Entry history

Integrated into UniProtKB/Swiss-Prot:	January 15, 2008
Last sequence update:	October 1, 2003
Last modified:	January 25, 2012
This is version 75 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program

Disclaimer

Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.