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Protein

Transcription initiation factor TFIID subunit 8

Gene

TAF8

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Transcription factor TFIID is one of the general factors required for accurate and regulated initiation by RNA polymerase II. Mediates both basal and activator-dependent transcription. Plays a role in the differentiation of preadipocyte fibroblasts to adipocytes, however, does not seem to play a role in differentiation of myoblasts. Required for the integration of TAF10 in the TAF complex. May be important for survival of cells of the inner cell mass which constitute the pluripotent cell population of the early embryo (By similarity).By similarity1 Publication

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Developmental protein

Keywords - Biological processi

Differentiation, Transcription, Transcription regulation

Enzyme and pathway databases

ReactomeiR-HSA-6807505. RNA polymerase II transcribes snRNA genes.

Names & Taxonomyi

Protein namesi
Recommended name:
Transcription initiation factor TFIID subunit 8
Alternative name(s):
Protein taube nuss
TBP-associated factor 43 kDa
TBP-associated factor 8
Transcription initiation factor TFIID 43 kDa subunit
Short name:
TAFII-43
Short name:
TAFII43
Short name:
hTAFII43
Gene namesi
Name:TAF8
Synonyms:TAFII43, TBN
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
Proteomesi
  • UP000005640 Componenti: Chromosome 6

Organism-specific databases

HGNCiHGNC:17300. TAF8.

Subcellular locationi

  • Nucleus 1 Publication
  • Cytoplasm By similarity

  • Note: Localized in the cytoplasm and transported from the cytoplasm to the nucleus in some cells, possibly depending on the functional or developmental state of the cell.By similarity

GO - Cellular componenti

  • nucleoplasm Source: HPA
  • nucleus Source: HGNC
  • perinuclear region of cytoplasm Source: HGNC
  • transcription factor TFIID complex Source: MGI
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm, Nucleus

Pathology & Biotechi

Organism-specific databases

PharmGKBiPA162405144.

Polymorphism and mutation databases

BioMutaiTAF8.
DMDMi74723384.

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Initiator methionineiRemovedCombined sources
Chaini2 – 310309Transcription initiation factor TFIID subunit 8PRO_0000315396Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei2 – 21N-acetylalanineCombined sources
Modified residuei130 – 1301PhosphothreonineCombined sources
Modified residuei271 – 2711PhosphoserineCombined sources

Keywords - PTMi

Acetylation, Phosphoprotein

Proteomic databases

EPDiQ7Z7C8.
MaxQBiQ7Z7C8.
PaxDbiQ7Z7C8.
PeptideAtlasiQ7Z7C8.
PRIDEiQ7Z7C8.

PTM databases

iPTMnetiQ7Z7C8.
PhosphoSiteiQ7Z7C8.

Expressioni

Inductioni

Induced with triglyceride accumulation.

Gene expression databases

BgeeiENSG00000137413.
CleanExiHS_TAF8.
ExpressionAtlasiQ7Z7C8. baseline and differential.
GenevisibleiQ7Z7C8. HS.

Organism-specific databases

HPAiHPA031730.
HPA031731.
HPA031734.

Interactioni

Subunit structurei

TFIID is composed of TATA binding protein (TBP) and a number of TBP-associated factors (TAFs). Interacts with TBP, TAF1, TAF6, TAF10, TAF11 and TAF13. Component also of a small TAF complex (SMAT) containing TAF8, TAF10 and SUPT7L. Forms a heterodimer with TAF10. Interaction with TAF10 is mediated mainly via its histone fold domain while interaction with SUPT7L is via its C-terminal region.3 Publications

Protein-protein interaction databases

BioGridi126205. 30 interactions.
DIPiDIP-60591N.
IntActiQ7Z7C8. 4 interactions.
STRINGi9606.ENSP00000362068.

Structurei

Secondary structure

1
310
Legend: HelixTurnBeta strand
Show more details
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Helixi29 – 4820Combined sources
Beta strandi51 – 533Combined sources
Helixi55 – 8026Combined sources
Helixi90 – 9910Combined sources
Helixi104 – 1063Combined sources
Helixi107 – 11610Combined sources

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
4WV4X-ray1.91B25-120[»]
4WV6X-ray1.75B/C297-310[»]
5FURelectron microscopy8.50L1-310[»]
ProteinModelPortaliQ7Z7C8.
SMRiQ7Z7C8. Positions 28-120.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini35 – 10268Histone-foldAdd
BLAST

Motif

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Motifi294 – 30714Nuclear localization signalSequence analysisAdd
BLAST

Domaini

Ectopic expression of the histone fold domain acts as a dominant-negative mutant resulting in differentiation inhibition.

Sequence similaritiesi

Belongs to the TAF8 family.Curated
Contains 1 histone-fold domain.Curated

Phylogenomic databases

eggNOGiKOG4336. Eukaryota.
ENOG410ZRN5. LUCA.
GeneTreeiENSGT00390000017567.
HOGENOMiHOG000007034.
HOVERGENiHBG105411.
InParanoidiQ7Z7C8.
KOiK14649.
OMAiRTMPTVG.
OrthoDBiEOG091G0O7T.
PhylomeDBiQ7Z7C8.
TreeFamiTF316311.

Family and domain databases

CDDicd08049. TAF8. 1 hit.
Gene3Di1.10.20.10. 1 hit.
InterProiIPR006565. BTP.
IPR009072. Histone-fold.
IPR019473. TFIID_su8_C.
[Graphical view]
PfamiPF07524. Bromo_TP. 1 hit.
PF10406. TAF8_C. 1 hit.
[Graphical view]
SMARTiSM00576. BTP. 1 hit.
[Graphical view]
SUPFAMiSSF47113. SSF47113. 1 hit.

Sequences (3)i

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

This entry describes 3 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 1 (identifier: Q7Z7C8-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MADAAATAGA GGSGTRSGSK QSTNPADNYH LARRRTLQVV VSSLLTEAGF
60 70 80 90 100
ESAEKASVET LTEMLQSYIS EIGRSAKSYC EHTARTQPTL SDIVVTLVEM
110 120 130 140 150
GFNVDTLPAY AKRSQRMVIT APPVTNQPVT PKALTAGQNR PHPPHIPSHF
160 170 180 190 200
PEFPDPHTYI KTPTYREPVS DYQVLREKAA SQRRDVERAL TRFMAKTGET
210 220 230 240 250
QSLFKDDVST FPLIAARPFT IPYLTALLPS ELEMQQMEET DSSEQDEQTD
260 270 280 290 300
TENLALHISM EDSGAEKENT SVLQQNPSLS GSRNGEENII DNPYLRPVKK
310
PKIRRKKSLS
Length:310
Mass (Da):34,262
Last modified:October 1, 2003 - v1
Checksum:i0F955828FE9FC0EA
GO
Isoform 2 (identifier: Q7Z7C8-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     308-310: SLS → PDTF

Show »
Length:311
Mass (Da):34,436
Checksum:iE54B88DAF8FE9FC0
GO
Isoform 3 (identifier: Q7Z7C8-4) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     261-310: EDSGAEKENT...PKIRRKKSLS → IESRSVTQAG...TSSITLICGR

Note: No experimental confirmation available.
Show »
Length:338
Mass (Da):37,411
Checksum:iB65123963AA96D63
GO

Sequence cautioni

The sequence BAB71460 differs from that shown. Reason: Frameshift at position 240. Curated

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti139 – 1391N → D in BAB71460 (PubMed:14702039).Curated

Alternative sequence

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Alternative sequencei261 – 31050EDSGA…KKSLS → IESRSVTQAGVQWQDLGSLQ PPPPGFKRFSSLSLLSSWNY RRILEPRRRTPLSCSRTPPC RVAGMGRRTSSITLICGR in isoform 3. 1 PublicationVSP_030548Add
BLAST
Alternative sequencei308 – 3103SLS → PDTF in isoform 2. 1 PublicationVSP_030549

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF465841 mRNA. Translation: AAP47094.1.
AK057383 mRNA. Translation: BAB71460.1. Frameshift.
AL513008, AL512274 Genomic DNA. Translation: CAI15202.1.
AL513008, AL512274 Genomic DNA. Translation: CAI15203.1.
AL512274, AL513008 Genomic DNA. Translation: CAI16781.1.
AL512274, AL513008 Genomic DNA. Translation: CAI16782.1.
BC119678 mRNA. Translation: AAI19679.1.
BC119679 mRNA. Translation: AAI19680.1.
CCDSiCCDS43462.1. [Q7Z7C8-1]
RefSeqiNP_612639.2. NM_138572.2. [Q7Z7C8-1]
XP_011512598.1. XM_011514296.1. [Q7Z7C8-4]
UniGeneiHs.520122.

Genome annotation databases

EnsembliENST00000372977; ENSP00000362068; ENSG00000137413. [Q7Z7C8-1]
ENST00000372982; ENSP00000362073; ENSG00000137413. [Q7Z7C8-4]
ENST00000456846; ENSP00000411900; ENSG00000137413. [Q7Z7C8-2]
ENST00000494547; ENSP00000417867; ENSG00000137413. [Q7Z7C8-4]
GeneIDi129685.
KEGGihsa:129685.
UCSCiuc003ors.4. human. [Q7Z7C8-1]

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF465841 mRNA. Translation: AAP47094.1.
AK057383 mRNA. Translation: BAB71460.1. Frameshift.
AL513008, AL512274 Genomic DNA. Translation: CAI15202.1.
AL513008, AL512274 Genomic DNA. Translation: CAI15203.1.
AL512274, AL513008 Genomic DNA. Translation: CAI16781.1.
AL512274, AL513008 Genomic DNA. Translation: CAI16782.1.
BC119678 mRNA. Translation: AAI19679.1.
BC119679 mRNA. Translation: AAI19680.1.
CCDSiCCDS43462.1. [Q7Z7C8-1]
RefSeqiNP_612639.2. NM_138572.2. [Q7Z7C8-1]
XP_011512598.1. XM_011514296.1. [Q7Z7C8-4]
UniGeneiHs.520122.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
4WV4X-ray1.91B25-120[»]
4WV6X-ray1.75B/C297-310[»]
5FURelectron microscopy8.50L1-310[»]
ProteinModelPortaliQ7Z7C8.
SMRiQ7Z7C8. Positions 28-120.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi126205. 30 interactions.
DIPiDIP-60591N.
IntActiQ7Z7C8. 4 interactions.
STRINGi9606.ENSP00000362068.

PTM databases

iPTMnetiQ7Z7C8.
PhosphoSiteiQ7Z7C8.

Polymorphism and mutation databases

BioMutaiTAF8.
DMDMi74723384.

Proteomic databases

EPDiQ7Z7C8.
MaxQBiQ7Z7C8.
PaxDbiQ7Z7C8.
PeptideAtlasiQ7Z7C8.
PRIDEiQ7Z7C8.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENST00000372977; ENSP00000362068; ENSG00000137413. [Q7Z7C8-1]
ENST00000372982; ENSP00000362073; ENSG00000137413. [Q7Z7C8-4]
ENST00000456846; ENSP00000411900; ENSG00000137413. [Q7Z7C8-2]
ENST00000494547; ENSP00000417867; ENSG00000137413. [Q7Z7C8-4]
GeneIDi129685.
KEGGihsa:129685.
UCSCiuc003ors.4. human. [Q7Z7C8-1]

Organism-specific databases

CTDi129685.
GeneCardsiTAF8.
HGNCiHGNC:17300. TAF8.
HPAiHPA031730.
HPA031731.
HPA031734.
MIMi609514. gene.
neXtProtiNX_Q7Z7C8.
PharmGKBiPA162405144.
GenAtlasiSearch...

Phylogenomic databases

eggNOGiKOG4336. Eukaryota.
ENOG410ZRN5. LUCA.
GeneTreeiENSGT00390000017567.
HOGENOMiHOG000007034.
HOVERGENiHBG105411.
InParanoidiQ7Z7C8.
KOiK14649.
OMAiRTMPTVG.
OrthoDBiEOG091G0O7T.
PhylomeDBiQ7Z7C8.
TreeFamiTF316311.

Enzyme and pathway databases

ReactomeiR-HSA-6807505. RNA polymerase II transcribes snRNA genes.

Miscellaneous databases

ChiTaRSiTAF8. human.
GeneWikiiTAF8.
GenomeRNAii129685.
PROiQ7Z7C8.
SOURCEiSearch...

Gene expression databases

BgeeiENSG00000137413.
CleanExiHS_TAF8.
ExpressionAtlasiQ7Z7C8. baseline and differential.
GenevisibleiQ7Z7C8. HS.

Family and domain databases

CDDicd08049. TAF8. 1 hit.
Gene3Di1.10.20.10. 1 hit.
InterProiIPR006565. BTP.
IPR009072. Histone-fold.
IPR019473. TFIID_su8_C.
[Graphical view]
PfamiPF07524. Bromo_TP. 1 hit.
PF10406. TAF8_C. 1 hit.
[Graphical view]
SMARTiSM00576. BTP. 1 hit.
[Graphical view]
SUPFAMiSSF47113. SSF47113. 1 hit.
ProtoNetiSearch...

Entry informationi

Entry nameiTAF8_HUMAN
AccessioniPrimary (citable) accession number: Q7Z7C8
Secondary accession number(s): Q5T0K1, Q8N4R9, Q96M52
Entry historyi
Integrated into UniProtKB/Swiss-Prot: January 15, 2008
Last sequence update: October 1, 2003
Last modified: September 7, 2016
This is version 114 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Miscellaneous

'Taube nuss' means "empty nut' in German.

Keywords - Technical termi

3D-structure, Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. Human chromosome 6
    Human chromosome 6: entries, gene names and cross-references to MIM
  2. MIM cross-references
    Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
  3. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  4. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.