Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Protein

PX domain-containing protein kinase-like protein

Gene

PXK

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Binds to and modulates brain Na,K-ATPase subunits ATP1B1 and ATP1B3 and may thereby participate in the regulation of electrical excitability and synaptic transmission. May not display kinase activity.1 PublicationBy similarity

GO - Molecular functioni

  • phosphatidylinositol binding Source: UniProtKB
  • protein C-terminus binding Source: UniProtKB

GO - Biological processi

  • inflammatory response Source: UniProtKB
  • modulation of synaptic transmission Source: UniProtKB
  • negative regulation of ATPase activity Source: UniProtKB
  • negative regulation of ion transport Source: UniProtKB
  • regulation of membrane potential Source: GO_Central
Complete GO annotation...

Keywords - Ligandi

Actin-binding

Enzyme and pathway databases

SignaLinkiQ7Z7A4.

Names & Taxonomyi

Protein namesi
Recommended name:
PX domain-containing protein kinase-like protein
Alternative name(s):
Modulator of Na,K-ATPase
Short name:
MONaKA
Gene namesi
Name:PXKImported
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
Proteomesi
  • UP000005640 Componenti: Chromosome 3

Organism-specific databases

HGNCiHGNC:23326. PXK.

Subcellular locationi

  • Cytoplasm 1 Publication
  • Cell membrane 1 Publication; Peripheral membrane protein 1 Publication

  • Note: Also associates with the plasma membrane. Isoform 3 is present throughout the cell.

GO - Cellular componenti

  • cytoplasm Source: UniProtKB
  • microtubule organizing center Source: HPA
  • nucleus Source: UniProtKB
  • plasma membrane Source: UniProtKB
  • protein complex Source: Ensembl
Complete GO annotation...

Keywords - Cellular componenti

Cell membrane, Cytoplasm, Membrane

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi54R → Q: No effect on subcellular location. 1 Publication1
Mutagenesisi56Y → A: Results in redistribution of protein from cytoplasm throughout entire cell. 1 Publication1
Mutagenesisi92R → L: Results in redistribution of protein from cytoplasm throughout entire cell. 1 Publication1

Organism-specific databases

DisGeNETi54899.
OpenTargetsiENSG00000168297.
Orphaneti536. Systemic lupus erythematosus.
PharmGKBiPA134899496.

Polymorphism and mutation databases

BioMutaiPXK.
DMDMi74759261.

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000865921 – 578PX domain-containing protein kinase-like proteinAdd BLAST578

Proteomic databases

EPDiQ7Z7A4.
MaxQBiQ7Z7A4.
PaxDbiQ7Z7A4.
PeptideAtlasiQ7Z7A4.
PRIDEiQ7Z7A4.

PTM databases

iPTMnetiQ7Z7A4.
PhosphoSitePlusiQ7Z7A4.

Expressioni

Tissue specificityi

Widely expressed in all tissues examined except in heart. Isoform 1 is expressed in high levels in the brain, skeletal muscle, spleen and testis. Isoform 7 expression has yet to be demonstrated.1 Publication

Gene expression databases

BgeeiENSG00000168297.
ExpressionAtlasiQ7Z7A4. baseline and differential.
GenevisibleiQ7Z7A4. HS.

Organism-specific databases

HPAiHPA024068.

Interactioni

GO - Molecular functioni

  • protein C-terminus binding Source: UniProtKB

Protein-protein interaction databases

BioGridi120245. 15 interactors.
IntActiQ7Z7A4. 4 interactors.
STRINGi9606.ENSP00000348472.

Structurei

3D structure databases

ProteinModelPortaliQ7Z7A4.
SMRiQ7Z7A4.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini14 – 126PXPROSITE-ProRule annotationAdd BLAST113
Domaini88 – 481Protein kinasePROSITE-ProRule annotationAdd BLAST394
Domaini548 – 567WH2PROSITE-ProRule annotationAdd BLAST20

Domaini

The protein kinase domain is predicted to be catalytically inactive.

Sequence similaritiesi

Belongs to the protein kinase superfamily.Curated
Contains 1 protein kinase domain.PROSITE-ProRule annotation
Contains 1 PX (phox homology) domain.PROSITE-ProRule annotation
Contains 1 WH2 domain.PROSITE-ProRule annotation

Phylogenomic databases

eggNOGiENOG410IF7B. Eukaryota.
ENOG410Z20X. LUCA.
GeneTreeiENSGT00390000017669.
HOVERGENiHBG061245.
InParanoidiQ7Z7A4.
KOiK17543.
OMAiLESICKF.
OrthoDBiEOG091G09MA.
PhylomeDBiQ7Z7A4.
TreeFamiTF324116.

Family and domain databases

Gene3Di3.30.1520.10. 1 hit.
InterProiIPR011009. Kinase-like_dom.
IPR001683. Phox.
IPR000719. Prot_kinase_dom.
IPR003124. WH2_dom.
[Graphical view]
PfamiPF00069. Pkinase. 1 hit.
PF00787. PX. 1 hit.
PF02205. WH2. 1 hit.
[Graphical view]
SMARTiSM00312. PX. 1 hit.
[Graphical view]
SUPFAMiSSF56112. SSF56112. 1 hit.
SSF64268. SSF64268. 1 hit.
PROSITEiPS50011. PROTEIN_KINASE_DOM. 1 hit.
PS50195. PX. 1 hit.
PS51082. WH2. 1 hit.
[Graphical view]

Sequences (7)i

Sequence statusi: Complete.

This entry describes 7 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 11 Publication (identifier: Q7Z7A4-1) [UniParc]FASTAAdd to basket
Also known as: Long1 Publication, v11 Publication

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MAFMEKPPAG KVLLDDTVPL TAAIEASQSL QSHTEYIIRV QRGISVENSW
60 70 80 90 100
QIVRRYSDFD LLNNSLQIAG LSLPLPPKKL IGNMDREFIA ERQKGLQNYL
110 120 130 140 150
NVITTNHILS NCELVKKFLD PNNYSANYTE IALQQVSMFF RSEPKWEVVE
160 170 180 190 200
PLKDIGWRIR KKYFLMKIKN QPKERLVLSW ADLGPDKYLS DKDFQCLIKL
210 220 230 240 250
LPSCLHPYIY RVTFATANES SALLIRMFNE KGTLKDLIYK AKPKDPFLKK
260 270 280 290 300
YCNPKKIQGL ELQQIKTYGR QILEVLKFLH DKGFPYGHLH ASNVMLDGDT
310 320 330 340 350
CRLLDLENSL LGLPSFYRSY FSQFRKINTL ESVDVHCFGH LLYEMTYGRP
360 370 380 390 400
PDSVPVDSFP PAPSMAVVAV LESTLSCEAC KNGMPTISRL LQMPLFSDVL
410 420 430 440 450
LTTSEKPQFK IPTKLKEALR IAKECIEKRL IEEQKQIHQH RRLTRAQSHH
460 470 480 490 500
GSEEERKKRK ILARKKSKRS ALENSEEHSA KYSNSNNSAG SGASSPLTSP
510 520 530 540 550
SSPTPPSTSG ISALPPPPPP PPPPAAPLPP ASTEAPAQLS SQAVNGMSRG
560 570
ALLSSIQNFQ KGTLRKAKTC DHSAPKIG
Length:578
Mass (Da):64,950
Last modified:October 1, 2003 - v1
Checksum:i73523CFC3731FFC8
GO
Isoform 21 Publication (identifier: Q7Z7A4-2) [UniParc]FASTAAdd to basket
Also known as: Short1 Publication

The sequence of this isoform differs from the canonical sequence as follows:
     510-515: GISALP → VEHAPF
     516-578: Missing.

Show »
Length:515
Mass (Da):58,732
Checksum:i8115EBC6A5157C97
GO
Isoform 31 Publication (identifier: Q7Z7A4-3) [UniParc]FASTAAdd to basket
Also known as: v31 Publication

The sequence of this isoform differs from the canonical sequence as follows:
     1-226: Missing.

Show »
Length:352
Mass (Da):38,875
Checksum:iDE5F73819ABBB77C
GO
Isoform 4Curated (identifier: Q7Z7A4-4) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     35-51: Missing.

Note: No experimental confirmation available.Curated
Show »
Length:561
Mass (Da):62,891
Checksum:i615A342917403DCD
GO
Isoform 51 Publication (identifier: Q7Z7A4-5) [UniParc]FASTAAdd to basket
Also known as: v41 Publication

The sequence of this isoform differs from the canonical sequence as follows:
     1-137: Missing.

Show »
Length:441
Mass (Da):49,491
Checksum:iF1D219D8C6988D86
GO
Isoform 61 Publication (identifier: Q7Z7A4-6) [UniParc]FASTAAdd to basket
Also known as: v21 Publication

The sequence of this isoform differs from the canonical sequence as follows:
     35-67: Missing.

Show »
Length:545
Mass (Da):60,956
Checksum:iA1F5BED8AEAFF293
GO
Isoform 71 Publication (identifier: Q7Z7A4-7) [UniParc]FASTAAdd to basket
Also known as: v51 Publication

The sequence of this isoform differs from the canonical sequence as follows:
     1-83: Missing.

Show »
Length:495
Mass (Da):55,762
Checksum:iE5BA2969CBC9E57D
GO

Sequence cautioni

The sequence AAH14479 differs from that shown. Reason: Erroneous termination at position 451. Translated as Gly.Curated
The sequence BAA91097 differs from that shown. Reason: Frameshift at position 569.Curated
The sequence BAD18536 differs from that shown. Reason: Frameshift at position 510.Curated
The sequence BAD92925 differs from that shown. Reason: Frameshift at position 147.Curated

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti42R → G in AAK94455 (Ref. 3) Curated1
Sequence conflicti42R → G in BAA91097 (PubMed:14702039).Curated1
Sequence conflicti459R → K in AAR98521 (PubMed:16142408).Curated1
Sequence conflicti489Missing in BAD18536 (PubMed:14702039).Curated1

Natural variant

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Natural variantiVAR_041362426I → V.1 PublicationCorresponds to variant rs55973253dbSNPEnsembl.1
Natural variantiVAR_041363481K → R.2 PublicationsCorresponds to variant rs56384862dbSNPEnsembl.1
Natural variantiVAR_033911525A → V.Corresponds to variant rs34579268dbSNPEnsembl.1
Natural variantiVAR_033912535A → V.Corresponds to variant rs34579268dbSNPEnsembl.1

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_0519071 – 226Missing in isoform 3. 1 PublicationAdd BLAST226
Alternative sequenceiVSP_0519081 – 137Missing in isoform 5. 1 PublicationAdd BLAST137
Alternative sequenceiVSP_0519091 – 83Missing in isoform 7. 1 PublicationAdd BLAST83
Alternative sequenceiVSP_05191035 – 67Missing in isoform 6. 1 PublicationAdd BLAST33
Alternative sequenceiVSP_05191135 – 51Missing in isoform 4. 1 PublicationAdd BLAST17
Alternative sequenceiVSP_051912510 – 515GISALP → VEHAPF in isoform 2. 3 Publications6
Alternative sequenceiVSP_051913516 – 578Missing in isoform 2. 3 PublicationsAdd BLAST63

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
DQ124707 mRNA. Translation: AAZ38821.1.
DQ124708 mRNA. Translation: AAZ38822.1.
AY437879 mRNA. Translation: AAR98521.1.
AY847222 mRNA. Translation: AAX73354.1.
AY847220 mRNA. Translation: AAX73352.1.
AY847221 mRNA. Translation: AAX73353.1.
AF399753 mRNA. Translation: AAK94455.1.
AY274811 mRNA. Translation: AAP42076.1.
AB209688 mRNA. Translation: BAD92925.1. Frameshift.
AK000342 mRNA. Translation: BAA91097.1. Frameshift.
AK131385 mRNA. Translation: BAD18536.1. Frameshift.
BC008943 mRNA. Translation: AAH08943.1.
BC014479 mRNA. Translation: AAH14479.1. Sequence problems.
CCDSiCCDS2889.1. [Q7Z7A4-1]
CCDS74952.1. [Q7Z7A4-2]
RefSeqiNP_001276024.1. NM_001289095.1.
NP_001276025.1. NM_001289096.1. [Q7Z7A4-6]
NP_001276027.1. NM_001289098.1. [Q7Z7A4-2]
NP_001276028.1. NM_001289099.1. [Q7Z7A4-7]
NP_001276029.1. NM_001289100.1.
NP_001276030.1. NM_001289101.1. [Q7Z7A4-5]
NP_060241.2. NM_017771.4. [Q7Z7A4-1]
XP_016862160.1. XM_017006671.1. [Q7Z7A4-4]
XP_016862164.1. XM_017006675.1. [Q7Z7A4-2]
XP_016862175.1. XM_017006686.1. [Q7Z7A4-7]
UniGeneiHs.190544.

Genome annotation databases

EnsembliENST00000356151; ENSP00000348472; ENSG00000168297. [Q7Z7A4-1]
ENST00000383716; ENSP00000373222; ENSG00000168297. [Q7Z7A4-2]
ENST00000484288; ENSP00000417915; ENSG00000168297. [Q7Z7A4-2]
GeneIDi54899.
KEGGihsa:54899.
UCSCiuc003djx.2. human. [Q7Z7A4-1]

Keywords - Coding sequence diversityi

Alternative splicing, Polymorphism

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
DQ124707 mRNA. Translation: AAZ38821.1.
DQ124708 mRNA. Translation: AAZ38822.1.
AY437879 mRNA. Translation: AAR98521.1.
AY847222 mRNA. Translation: AAX73354.1.
AY847220 mRNA. Translation: AAX73352.1.
AY847221 mRNA. Translation: AAX73353.1.
AF399753 mRNA. Translation: AAK94455.1.
AY274811 mRNA. Translation: AAP42076.1.
AB209688 mRNA. Translation: BAD92925.1. Frameshift.
AK000342 mRNA. Translation: BAA91097.1. Frameshift.
AK131385 mRNA. Translation: BAD18536.1. Frameshift.
BC008943 mRNA. Translation: AAH08943.1.
BC014479 mRNA. Translation: AAH14479.1. Sequence problems.
CCDSiCCDS2889.1. [Q7Z7A4-1]
CCDS74952.1. [Q7Z7A4-2]
RefSeqiNP_001276024.1. NM_001289095.1.
NP_001276025.1. NM_001289096.1. [Q7Z7A4-6]
NP_001276027.1. NM_001289098.1. [Q7Z7A4-2]
NP_001276028.1. NM_001289099.1. [Q7Z7A4-7]
NP_001276029.1. NM_001289100.1.
NP_001276030.1. NM_001289101.1. [Q7Z7A4-5]
NP_060241.2. NM_017771.4. [Q7Z7A4-1]
XP_016862160.1. XM_017006671.1. [Q7Z7A4-4]
XP_016862164.1. XM_017006675.1. [Q7Z7A4-2]
XP_016862175.1. XM_017006686.1. [Q7Z7A4-7]
UniGeneiHs.190544.

3D structure databases

ProteinModelPortaliQ7Z7A4.
SMRiQ7Z7A4.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi120245. 15 interactors.
IntActiQ7Z7A4. 4 interactors.
STRINGi9606.ENSP00000348472.

PTM databases

iPTMnetiQ7Z7A4.
PhosphoSitePlusiQ7Z7A4.

Polymorphism and mutation databases

BioMutaiPXK.
DMDMi74759261.

Proteomic databases

EPDiQ7Z7A4.
MaxQBiQ7Z7A4.
PaxDbiQ7Z7A4.
PeptideAtlasiQ7Z7A4.
PRIDEiQ7Z7A4.

Protocols and materials databases

DNASUi54899.
Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENST00000356151; ENSP00000348472; ENSG00000168297. [Q7Z7A4-1]
ENST00000383716; ENSP00000373222; ENSG00000168297. [Q7Z7A4-2]
ENST00000484288; ENSP00000417915; ENSG00000168297. [Q7Z7A4-2]
GeneIDi54899.
KEGGihsa:54899.
UCSCiuc003djx.2. human. [Q7Z7A4-1]

Organism-specific databases

CTDi54899.
DisGeNETi54899.
GeneCardsiPXK.
H-InvDBHIX0020846.
HGNCiHGNC:23326. PXK.
HPAiHPA024068.
MIMi611450. gene.
neXtProtiNX_Q7Z7A4.
OpenTargetsiENSG00000168297.
Orphaneti536. Systemic lupus erythematosus.
PharmGKBiPA134899496.
GenAtlasiSearch...

Phylogenomic databases

eggNOGiENOG410IF7B. Eukaryota.
ENOG410Z20X. LUCA.
GeneTreeiENSGT00390000017669.
HOVERGENiHBG061245.
InParanoidiQ7Z7A4.
KOiK17543.
OMAiLESICKF.
OrthoDBiEOG091G09MA.
PhylomeDBiQ7Z7A4.
TreeFamiTF324116.

Enzyme and pathway databases

SignaLinkiQ7Z7A4.

Miscellaneous databases

ChiTaRSiPXK. human.
GenomeRNAii54899.
PROiQ7Z7A4.
SOURCEiSearch...

Gene expression databases

BgeeiENSG00000168297.
ExpressionAtlasiQ7Z7A4. baseline and differential.
GenevisibleiQ7Z7A4. HS.

Family and domain databases

Gene3Di3.30.1520.10. 1 hit.
InterProiIPR011009. Kinase-like_dom.
IPR001683. Phox.
IPR000719. Prot_kinase_dom.
IPR003124. WH2_dom.
[Graphical view]
PfamiPF00069. Pkinase. 1 hit.
PF00787. PX. 1 hit.
PF02205. WH2. 1 hit.
[Graphical view]
SMARTiSM00312. PX. 1 hit.
[Graphical view]
SUPFAMiSSF56112. SSF56112. 1 hit.
SSF64268. SSF64268. 1 hit.
PROSITEiPS50011. PROTEIN_KINASE_DOM. 1 hit.
PS50195. PX. 1 hit.
PS51082. WH2. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiPXK_HUMAN
AccessioniPrimary (citable) accession number: Q7Z7A4
Secondary accession number(s): Q3BCH4
, Q3BCH5, Q3BCH6, Q3BDW1, Q45L83, Q59EX3, Q6PK17, Q6ZN39, Q96CA3, Q96R07, Q9NXB8
Entry historyi
Integrated into UniProtKB/Swiss-Prot: December 6, 2005
Last sequence update: October 1, 2003
Last modified: November 2, 2016
This is version 123 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Human chromosome 3
    Human chromosome 3: entries, gene names and cross-references to MIM
  2. Human entries with polymorphisms or disease mutations
    List of human entries with polymorphisms or disease mutations
  3. Human polymorphisms and disease mutations
    Index of human polymorphisms and disease mutations
  4. MIM cross-references
    Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
  5. Human and mouse protein kinases
    Human and mouse protein kinases: classification and index
  6. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.