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Q7Z7A4 (PXK_HUMAN) Reviewed, UniProtKB/Swiss-Prot

Last modified July 9, 2014. Version 105. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (6) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Alt products·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
PX domain-containing protein kinase-like protein
Alternative name(s):
Modulator of Na,K-ATPase
Short name=MONaKA
Gene names
Name:PXK
OrganismHomo sapiens (Human) [Reference proteome]
Taxonomic identifier9606 [NCBI]
Taxonomic lineageEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo

Protein attributes

Sequence length578 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Binds to and modulates brain Na,K-ATPase subunits ATP1B1 and ATP1B3 and may thereby participate in the regulation of electrical excitability and synaptic transmission. May not display kinase activity. Ref.2 UniProtKB Q8BX57

Subcellular location

Cytoplasm. Cell membrane; Peripheral membrane protein. Note: Also associates with the plasma membrane. Isoform 3 is present throughout the cell. Ref.2

Tissue specificity

Widely expressed in all tissues examined except in heart. Isoform 1 is expressed in high levels in the brain, skeletal muscle, spleen and testis. Isoform 7 expression has yet to be demonstrated. Ref.2

Domain

The protein kinase domain is predicted to be catalytically inactive.

Sequence similarities

Belongs to the protein kinase superfamily.

Contains 1 protein kinase domain.

Contains 1 PX (phox homology) domain.

Contains 1 WH2 domain.

Sequence caution

The sequence AAH14479.1 differs from that shown. Reason: Erroneous termination at position 451. Translated as Gly.

The sequence BAA91097.1 differs from that shown. Reason: Frameshift at position 569.

The sequence BAD18536.1 differs from that shown. Reason: Frameshift at position 510.

The sequence BAD92925.1 differs from that shown. Reason: Frameshift at position 147.

Alternative products

This entry describes 7 isoforms produced by alternative splicing. [Align] [Select]
Isoform 1 Ref.1 (identifier: Q7Z7A4-1)

Also known as: Long; v1;

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.
Isoform 2 Ref.1 (identifier: Q7Z7A4-2)

Also known as: Short;

The sequence of this isoform differs from the canonical sequence as follows:
     510-515: GISALP → VEHAPF
     516-578: Missing.
Isoform 3 Ref.2 (identifier: Q7Z7A4-3)

Also known as: v3;

The sequence of this isoform differs from the canonical sequence as follows:
     1-226: Missing.
Isoform 4 (identifier: Q7Z7A4-4)

The sequence of this isoform differs from the canonical sequence as follows:
     35-51: Missing.
Note: No experimental confirmation available.
Isoform 5 Ref.2 (identifier: Q7Z7A4-5)

Also known as: v4;

The sequence of this isoform differs from the canonical sequence as follows:
     1-137: Missing.
Isoform 6 Ref.2 (identifier: Q7Z7A4-6)

Also known as: v2;

The sequence of this isoform differs from the canonical sequence as follows:
     35-67: Missing.
Isoform 7 Ref.2 (identifier: Q7Z7A4-7)

Also known as: v5;

The sequence of this isoform differs from the canonical sequence as follows:
     1-83: Missing.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 578578PX domain-containing protein kinase-like protein
PRO_0000086592

Regions

Domain14 – 126113PX
Domain88 – 481394Protein kinase
Domain548 – 56720WH2

Natural variations

Alternative sequence1 – 226226Missing in isoform 3. Ref.2
VSP_051907
Alternative sequence1 – 137137Missing in isoform 5. Ref.2
VSP_051908
Alternative sequence1 – 8383Missing in isoform 7. Ref.2
VSP_051909
Alternative sequence35 – 6733Missing in isoform 6. Ref.2
VSP_051910
Alternative sequence35 – 5117Missing in isoform 4.
VSP_051911
Alternative sequence510 – 5156GISALP → VEHAPF in isoform 2. Ref.1
VSP_051912
Alternative sequence516 – 57863Missing in isoform 2. Ref.1
VSP_051913
Natural variant4261I → V. Ref.9
Corresponds to variant rs55973253 [ dbSNP | Ensembl ].
VAR_041362
Natural variant4811K → R. Ref.2 Ref.9
Corresponds to variant rs56384862 [ dbSNP | Ensembl ].
VAR_041363
Natural variant5251A → V.
Corresponds to variant rs34579268 [ dbSNP | Ensembl ].
VAR_033911
Natural variant5351A → V.
Corresponds to variant rs34579268 [ dbSNP | Ensembl ].
VAR_033912

Experimental info

Mutagenesis541R → Q: No effect on subcellular location. Ref.2
Mutagenesis561Y → A: Results in redistribution of protein from cytoplasm throughout entire cell. Ref.2
Mutagenesis921R → L: Results in redistribution of protein from cytoplasm throughout entire cell. Ref.2
Sequence conflict421R → G in AAK94455. Ref.3
Sequence conflict421R → G in BAA91097. Ref.6
Sequence conflict4591R → K in AAR98521. Ref.2
Sequence conflict4891Missing in BAD18536. Ref.6

Sequences

Sequence LengthMass (Da)Tools
Isoform 1 (Long) (v1) [UniParc].

Last modified October 1, 2003. Version 1.
Checksum: 73523CFC3731FFC8

FASTA57864,950
        10         20         30         40         50         60 
MAFMEKPPAG KVLLDDTVPL TAAIEASQSL QSHTEYIIRV QRGISVENSW QIVRRYSDFD 

        70         80         90        100        110        120 
LLNNSLQIAG LSLPLPPKKL IGNMDREFIA ERQKGLQNYL NVITTNHILS NCELVKKFLD 

       130        140        150        160        170        180 
PNNYSANYTE IALQQVSMFF RSEPKWEVVE PLKDIGWRIR KKYFLMKIKN QPKERLVLSW 

       190        200        210        220        230        240 
ADLGPDKYLS DKDFQCLIKL LPSCLHPYIY RVTFATANES SALLIRMFNE KGTLKDLIYK 

       250        260        270        280        290        300 
AKPKDPFLKK YCNPKKIQGL ELQQIKTYGR QILEVLKFLH DKGFPYGHLH ASNVMLDGDT 

       310        320        330        340        350        360 
CRLLDLENSL LGLPSFYRSY FSQFRKINTL ESVDVHCFGH LLYEMTYGRP PDSVPVDSFP 

       370        380        390        400        410        420 
PAPSMAVVAV LESTLSCEAC KNGMPTISRL LQMPLFSDVL LTTSEKPQFK IPTKLKEALR 

       430        440        450        460        470        480 
IAKECIEKRL IEEQKQIHQH RRLTRAQSHH GSEEERKKRK ILARKKSKRS ALENSEEHSA 

       490        500        510        520        530        540 
KYSNSNNSAG SGASSPLTSP SSPTPPSTSG ISALPPPPPP PPPPAAPLPP ASTEAPAQLS 

       550        560        570 
SQAVNGMSRG ALLSSIQNFQ KGTLRKAKTC DHSAPKIG 

« Hide

Isoform 2 (Short) [UniParc].

Checksum: 8115EBC6A5157C97
Show »

FASTA51558,732
Isoform 3 (v3) [UniParc].

Checksum: DE5F73819ABBB77C
Show »

FASTA35238,875
Isoform 4 [UniParc].

Checksum: 615A342917403DCD
Show »

FASTA56162,891
Isoform 5 (v4) [UniParc].

Checksum: F1D219D8C6988D86
Show »

FASTA44149,491
Isoform 6 (v2) [UniParc].

Checksum: A1F5BED8AEAFF293
Show »

FASTA54560,956
Isoform 7 (v5) [UniParc].

Checksum: E5BA2969CBC9E57D
Show »

FASTA49555,762

References

« Hide 'large scale' references
[1]"MONaKA, a novel modulator of the plasma membrane Na,K-ATPase."
Mao H., Ferguson T.S., Cibulsky S.M., Holmqvist M., Ding C., Fei H., Levitan I.B.
J. Neurosci. 25:7934-7943(2005) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORMS 1 AND 2).
Tissue: Brain.
[2]"Expression pattern and subcellular localization of five splice isoforms of human PXK."
Zou X., Qiu G., Chen C., Wu M., Hu Y., Zheng H., Li X., Gu S., Ji C., Mao Y.
Int. J. Mol. Med. 16:701-707(2005) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORMS 1; 3; 5; 6 AND 7), TISSUE SPECIFICITY, SUBCELLULAR LOCATION, MUTAGENESIS OF ARG-54; TYR-56 AND ARG-92, VARIANT ARG-481.
Tissue: Fetal brain.
[3]Hong W.
Submitted (JUL-2001) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 2).
[4]Shan Y.X., Yu L.
Submitted (APR-2003) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1).
[5]Totoki Y., Toyoda A., Takeda T., Sakaki Y., Tanaka A., Yokoyama S., Ohara O., Nagase T., Kikuno R.F.
Submitted (MAR-2005) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 2).
Tissue: Brain.
[6]"Complete sequencing and characterization of 21,243 full-length human cDNAs."
Ota T., Suzuki Y., Nishikawa T., Otsuki T., Sugiyama T., Irie R., Wakamatsu A., Hayashi K., Sato H., Nagai K., Kimura K., Makita H., Sekine M., Obayashi M., Nishi T., Shibahara T., Tanaka T., Ishii S. expand/collapse author list , Yamamoto J., Saito K., Kawai Y., Isono Y., Nakamura Y., Nagahari K., Murakami K., Yasuda T., Iwayanagi T., Wagatsuma M., Shiratori A., Sudo H., Hosoiri T., Kaku Y., Kodaira H., Kondo H., Sugawara M., Takahashi M., Kanda K., Yokoi T., Furuya T., Kikkawa E., Omura Y., Abe K., Kamihara K., Katsuta N., Sato K., Tanikawa M., Yamazaki M., Ninomiya K., Ishibashi T., Yamashita H., Murakawa K., Fujimori K., Tanai H., Kimata M., Watanabe M., Hiraoka S., Chiba Y., Ishida S., Ono Y., Takiguchi S., Watanabe S., Yosida M., Hotuta T., Kusano J., Kanehori K., Takahashi-Fujii A., Hara H., Tanase T.-O., Nomura Y., Togiya S., Komai F., Hara R., Takeuchi K., Arita M., Imose N., Musashino K., Yuuki H., Oshima A., Sasaki N., Aotsuka S., Yoshikawa Y., Matsunawa H., Ichihara T., Shiohata N., Sano S., Moriya S., Momiyama H., Satoh N., Takami S., Terashima Y., Suzuki O., Nakagawa S., Senoh A., Mizoguchi H., Goto Y., Shimizu F., Wakebe H., Hishigaki H., Watanabe T., Sugiyama A., Takemoto M., Kawakami B., Yamazaki M., Watanabe K., Kumagai A., Itakura S., Fukuzumi Y., Fujimori Y., Komiyama M., Tashiro H., Tanigami A., Fujiwara T., Ono T., Yamada K., Fujii Y., Ozaki K., Hirao M., Ohmori Y., Kawabata A., Hikiji T., Kobatake N., Inagaki H., Ikema Y., Okamoto S., Okitani R., Kawakami T., Noguchi S., Itoh T., Shigeta K., Senba T., Matsumura K., Nakajima Y., Mizuno T., Morinaga M., Sasaki M., Togashi T., Oyama M., Hata H., Watanabe M., Komatsu T., Mizushima-Sugano J., Satoh T., Shirai Y., Takahashi Y., Nakagawa K., Okumura K., Nagase T., Nomura N., Kikuchi H., Masuho Y., Yamashita R., Nakai K., Yada T., Nakamura Y., Ohara O., Isogai T., Sugano S.
Nat. Genet. 36:40-45(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORMS 1 AND 4).
Tissue: Caudate nucleus and Hepatoma.
[7]"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
The MGC Project Team
Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 1-461 (ISOFORMS 1/2).
Tissue: B-cell and Kidney.
[8]"Quantitative phosphoproteomic analysis of T cell receptor signaling reveals system-wide modulation of protein-protein interactions."
Mayya V., Lundgren D.H., Hwang S.-I., Rezaul K., Wu L., Eng J.K., Rodionov V., Han D.K.
Sci. Signal. 2:RA46-RA46(2009) [PubMed] [Europe PMC] [Abstract]
Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
Tissue: Leukemic T-cell.
[9]"Patterns of somatic mutation in human cancer genomes."
Greenman C., Stephens P., Smith R., Dalgliesh G.L., Hunter C., Bignell G., Davies H., Teague J., Butler A., Stevens C., Edkins S., O'Meara S., Vastrik I., Schmidt E.E., Avis T., Barthorpe S., Bhamra G., Buck G. expand/collapse author list , Choudhury B., Clements J., Cole J., Dicks E., Forbes S., Gray K., Halliday K., Harrison R., Hills K., Hinton J., Jenkinson A., Jones D., Menzies A., Mironenko T., Perry J., Raine K., Richardson D., Shepherd R., Small A., Tofts C., Varian J., Webb T., West S., Widaa S., Yates A., Cahill D.P., Louis D.N., Goldstraw P., Nicholson A.G., Brasseur F., Looijenga L., Weber B.L., Chiew Y.-E., DeFazio A., Greaves M.F., Green A.R., Campbell P., Birney E., Easton D.F., Chenevix-Trench G., Tan M.-H., Khoo S.K., Teh B.T., Yuen S.T., Leung S.Y., Wooster R., Futreal P.A., Stratton M.R.
Nature 446:153-158(2007) [PubMed] [Europe PMC] [Abstract]
Cited for: VARIANTS [LARGE SCALE ANALYSIS] VAL-426 AND ARG-481.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
DQ124707 mRNA. Translation: AAZ38821.1.
DQ124708 mRNA. Translation: AAZ38822.1.
AY437879 mRNA. Translation: AAR98521.1.
AY847222 mRNA. Translation: AAX73354.1.
AY847220 mRNA. Translation: AAX73352.1.
AY847221 mRNA. Translation: AAX73353.1.
AF399753 mRNA. Translation: AAK94455.1.
AY274811 mRNA. Translation: AAP42076.1.
AB209688 mRNA. Translation: BAD92925.1. Frameshift.
AK000342 mRNA. Translation: BAA91097.1. Frameshift.
AK131385 mRNA. Translation: BAD18536.1. Frameshift.
BC008943 mRNA. Translation: AAH08943.1.
BC014479 mRNA. Translation: AAH14479.1. Sequence problems.
CCDSCCDS2889.1. [Q7Z7A4-1]
RefSeqNP_001276024.1. NM_001289095.1.
NP_001276025.1. NM_001289096.1. [Q7Z7A4-6]
NP_001276027.1. NM_001289098.1. [Q7Z7A4-2]
NP_001276028.1. NM_001289099.1. [Q7Z7A4-7]
NP_001276029.1. NM_001289100.1.
NP_001276030.1. NM_001289101.1. [Q7Z7A4-5]
NP_060241.2. NM_017771.4. [Q7Z7A4-1]
UniGeneHs.190544.

3D structure databases

ProteinModelPortalQ7Z7A4.
SMRQ7Z7A4. Positions 28-100.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

BioGrid120245. 6 interactions.

PTM databases

PhosphoSiteQ7Z7A4.

Polymorphism databases

DMDM74759261.

Proteomic databases

MaxQBQ7Z7A4.
PaxDbQ7Z7A4.
PRIDEQ7Z7A4.

Protocols and materials databases

DNASU54899.
StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblENST00000302779; ENSP00000305045; ENSG00000168297. [Q7Z7A4-4]
ENST00000356151; ENSP00000348472; ENSG00000168297. [Q7Z7A4-1]
ENST00000383716; ENSP00000373222; ENSG00000168297. [Q7Z7A4-6]
ENST00000479241; ENSP00000419049; ENSG00000168297.
ENST00000484288; ENSP00000417915; ENSG00000168297. [Q7Z7A4-2]
ENST00000536660; ENSP00000438356; ENSG00000168297. [Q7Z7A4-5]
GeneID54899.
KEGGhsa:54899.
UCSCuc003djx.1. human. [Q7Z7A4-2]
uc003djz.1. human. [Q7Z7A4-1]
uc010hnj.1. human. [Q7Z7A4-6]

Organism-specific databases

CTD54899.
GeneCardsGC03P058318.
H-InvDBHIX0020846.
HGNCHGNC:23326. PXK.
HPAHPA024068.
MIM611450. gene.
neXtProtNX_Q7Z7A4.
Orphanet536. Systemic lupus erythematosus.
PharmGKBPA134899496.
GenAtlasSearch...

Phylogenomic databases

eggNOGNOG71043.
HOVERGENHBG061245.
InParanoidQ7Z7A4.
KOK17543.
OMAPQAVNGV.
OrthoDBEOG7FFMRQ.
PhylomeDBQ7Z7A4.
TreeFamTF324116.

Enzyme and pathway databases

SignaLinkQ7Z7A4.

Gene expression databases

ArrayExpressQ7Z7A4.
BgeeQ7Z7A4.
GenevestigatorQ7Z7A4.

Family and domain databases

Gene3D3.30.1520.10. 1 hit.
InterProIPR011009. Kinase-like_dom.
IPR001683. Phox.
IPR000719. Prot_kinase_dom.
IPR003124. WH2_dom.
[Graphical view]
PfamPF00069. Pkinase. 1 hit.
PF00787. PX. 1 hit.
PF02205. WH2. 1 hit.
[Graphical view]
SMARTSM00312. PX. 1 hit.
[Graphical view]
SUPFAMSSF56112. SSF56112. 1 hit.
SSF64268. SSF64268. 1 hit.
PROSITEPS50011. PROTEIN_KINASE_DOM. 1 hit.
PS50195. PX. 1 hit.
PS51082. WH2. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

ChiTaRSPXK. human.
GenomeRNAi54899.
NextBio57920.
PROQ7Z7A4.
SOURCESearch...

Entry information

Entry namePXK_HUMAN
AccessionPrimary (citable) accession number: Q7Z7A4
Secondary accession number(s): Q3BCH4 expand/collapse secondary AC list , Q3BCH5, Q3BCH6, Q3BDW1, Q45L83, Q59EX3, Q6PK17, Q6ZN39, Q96CA3, Q96R07, Q9NXB8
Entry history
Integrated into UniProtKB/Swiss-Prot: December 6, 2005
Last sequence update: October 1, 2003
Last modified: July 9, 2014
This is version 105 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Relevant documents

SIMILARITY comments

Index of protein domains and families

Human and mouse protein kinases

Human and mouse protein kinases: classification and index

MIM cross-references

Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot

Human polymorphisms and disease mutations

Index of human polymorphisms and disease mutations

Human entries with polymorphisms or disease mutations

List of human entries with polymorphisms or disease mutations

Human chromosome 3

Human chromosome 3: entries, gene names and cross-references to MIM