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Q7Z7A3

- CTU1_HUMAN

UniProt

Q7Z7A3 - CTU1_HUMAN

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Protein
Cytoplasmic tRNA 2-thiolation protein 1
Gene
CTU1, ATPBD3, NCS6
Organism
Homo sapiens (Human)
Status
Reviewed - Annotation score: 5 out of 5 - Experimental evidence at protein leveli

Functioni

Plays a central role in 2-thiolation of mcm5S2U at tRNA wobble positions of tRNA(Lys), tRNA(Glu) and tRNA(Gln). Directly binds tRNAs and probably acts by catalyzing adenylation of tRNAs, an intermediate required for 2-thiolation. It is unclear whether it acts as a sulfurtransferase that transfers sulfur from thiocarboxylated URM1 onto the uridine of tRNAs at wobble position.1 Publication

Pathwayi

GO - Molecular functioni

  1. nucleotidyltransferase activity Source: UniProtKB-HAMAP
  2. protein binding Source: UniProtKB
  3. tRNA binding Source: UniProtKB-HAMAP

GO - Biological processi

  1. protein urmylation Source: UniProtKB-HAMAP
  2. tRNA thio-modification Source: UniProtKB
  3. tRNA wobble uridine modification Source: UniProtKB
Complete GO annotation...

Keywords - Molecular functioni

Transferase

Keywords - Biological processi

tRNA processing

Keywords - Ligandi

RNA-binding, tRNA-binding

Enzyme and pathway databases

UniPathwayiUPA00988.

Names & Taxonomyi

Protein namesi
Recommended name:
Cytoplasmic tRNA 2-thiolation protein 1 (EC:2.7.7.-)
Alternative name(s):
ATP-binding domain-containing protein 3
Cancer-associated gene protein
Cytoplasmic tRNA adenylyltransferase 1
Gene namesi
Name:CTU1
Synonyms:ATPBD3, NCS6
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
ProteomesiUP000005640: Chromosome 19

Organism-specific databases

HGNCiHGNC:29590. CTU1.

Subcellular locationi

Cytoplasm UniRule annotation

GO - Cellular componenti

  1. cytosol Source: UniProtKB
  2. mitochondrion Source: Ensembl
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

Pathology & Biotechi

Organism-specific databases

PharmGKBiPA165393368.

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 348348Cytoplasmic tRNA 2-thiolation protein 1UniRule annotation
PRO_0000282391Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei200 – 2001Phosphoserine3 Publications

Keywords - PTMi

Phosphoprotein

Proteomic databases

MaxQBiQ7Z7A3.
PaxDbiQ7Z7A3.
PRIDEiQ7Z7A3.

PTM databases

PhosphoSiteiQ7Z7A3.

Expressioni

Gene expression databases

BgeeiQ7Z7A3.
CleanExiHS_ATPBD3.
GenevestigatoriQ7Z7A3.

Organism-specific databases

HPAiHPA053797.

Interactioni

Subunit structurei

Component of a complex at least composed of URM1, CTU2/NCS2 and CTU1/ATPBD3. May form a heterodimer with CTU2/NCS2.1 Publication

Protein-protein interaction databases

BioGridi124701. 5 interactions.
DIPiDIP-48632N.
STRINGi9606.ENSP00000270637.

Structurei

3D structure databases

ProteinModelPortaliQ7Z7A3.
SMRiQ7Z7A3. Positions 46-254.

Family & Domainsi

Sequence similaritiesi

Phylogenomic databases

eggNOGiCOG0037.
HOGENOMiHOG000225864.
HOVERGENiHBG100428.
InParanoidiQ7Z7A3.
KOiK14168.
OMAiICTQGED.
OrthoDBiEOG7PGDRH.
PhylomeDBiQ7Z7A3.
TreeFamiTF352405.

Family and domain databases

Gene3Di3.40.50.620. 1 hit.
HAMAPiMF_03053. CTU1.
InterProiIPR012089. 2-thiocytidine_tRNA_synth_TtcA.
IPR000541. Ncs6/Tuc1/Ctu1.
IPR014729. Rossmann-like_a/b/a_fold.
IPR011063. tRNA-lysidine/thiocyt_synth.
[Graphical view]
PfamiPF01171. ATP_bind_3. 1 hit.
[Graphical view]
PIRSFiPIRSF004976. ATPase_YdaO. 1 hit.

Sequencei

Sequence statusi: Complete.

Q7Z7A3-1 [UniParc]FASTAAdd to Basket

« Hide

MPAPPCASCH AARAALRRPL SGQALCGACF CAAFEAEVLH TVLAGRLLPP    50
GAVVAVGASG GKDSTVLAHV LRALAPRLGI SLQLVAVDEG IGGYRDAALA 100
AVRRQAARWE LPLTVVAYED LFGGWTMDAV ARSTAGSGRS RSCCTFCGVL 150
RRRALEEGAR RVGATHIVTG HNADDMAETV LMNFLRGDAG RLARGGGLGS 200
PGEGGALPRC RPLQFASQKE VVLYAHFRRL DYFSEECVYA PEAFRGHARD 250
LLKRLEAARP SAVLDLVHSA ERLALAPAAR PPRPGACSRC GALASRALCQ 300
ACALLDGLNR GRPRLAIGKG RRGLDEEATP GTPGDPARPP ASKAVPTF 348
Length:348
Mass (Da):36,450
Last modified:October 1, 2003 - v1
Checksum:i2BA258902B6CA142
GO

Natural variant

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Natural varianti107 – 1071A → V.1 Publication
Corresponds to variant rs17855403 [ dbSNP | Ensembl ].
VAR_031402

Sequence conflict

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti71 – 711L → R in AAH09037. 1 Publication

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AY279382 Genomic DNA. Translation: AAP42277.1.
AK291173 mRNA. Translation: BAF83862.1.
CH471135 Genomic DNA. Translation: EAW71984.1.
BC009037 mRNA. Translation: AAH09037.1.
CCDSiCCDS12824.1.
RefSeqiNP_660275.2. NM_145232.3.
UniGeneiHs.148425.

Genome annotation databases

EnsembliENST00000421832; ENSP00000390011; ENSG00000142544.
GeneIDi90353.
KEGGihsa:90353.
UCSCiuc010eop.3. human.

Polymorphism databases

DMDMi74713747.

Keywords - Coding sequence diversityi

Polymorphism

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AY279382 Genomic DNA. Translation: AAP42277.1 .
AK291173 mRNA. Translation: BAF83862.1 .
CH471135 Genomic DNA. Translation: EAW71984.1 .
BC009037 mRNA. Translation: AAH09037.1 .
CCDSi CCDS12824.1.
RefSeqi NP_660275.2. NM_145232.3.
UniGenei Hs.148425.

3D structure databases

ProteinModelPortali Q7Z7A3.
SMRi Q7Z7A3. Positions 46-254.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

BioGridi 124701. 5 interactions.
DIPi DIP-48632N.
STRINGi 9606.ENSP00000270637.

PTM databases

PhosphoSitei Q7Z7A3.

Polymorphism databases

DMDMi 74713747.

Proteomic databases

MaxQBi Q7Z7A3.
PaxDbi Q7Z7A3.
PRIDEi Q7Z7A3.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

Ensembli ENST00000421832 ; ENSP00000390011 ; ENSG00000142544 .
GeneIDi 90353.
KEGGi hsa:90353.
UCSCi uc010eop.3. human.

Organism-specific databases

CTDi 90353.
GeneCardsi GC19M051601.
H-InvDB HIX0015377.
HIX0137480.
HGNCi HGNC:29590. CTU1.
HPAi HPA053797.
MIMi 612694. gene.
neXtProti NX_Q7Z7A3.
PharmGKBi PA165393368.
GenAtlasi Search...

Phylogenomic databases

eggNOGi COG0037.
HOGENOMi HOG000225864.
HOVERGENi HBG100428.
InParanoidi Q7Z7A3.
KOi K14168.
OMAi ICTQGED.
OrthoDBi EOG7PGDRH.
PhylomeDBi Q7Z7A3.
TreeFami TF352405.

Enzyme and pathway databases

UniPathwayi UPA00988 .

Miscellaneous databases

GenomeRNAii 90353.
NextBioi 76697.
PROi Q7Z7A3.
SOURCEi Search...

Gene expression databases

Bgeei Q7Z7A3.
CleanExi HS_ATPBD3.
Genevestigatori Q7Z7A3.

Family and domain databases

Gene3Di 3.40.50.620. 1 hit.
HAMAPi MF_03053. CTU1.
InterProi IPR012089. 2-thiocytidine_tRNA_synth_TtcA.
IPR000541. Ncs6/Tuc1/Ctu1.
IPR014729. Rossmann-like_a/b/a_fold.
IPR011063. tRNA-lysidine/thiocyt_synth.
[Graphical view ]
Pfami PF01171. ATP_bind_3. 1 hit.
[Graphical view ]
PIRSFi PIRSF004976. ATPase_YdaO. 1 hit.
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "Molecular characterization of a novel gene differentially expressed in breast and prostate cancers."
    Yousef G.M., Borgono C.A., Davidian C.T., Michael I., Diamandis E.P.
    Submitted (APR-2003) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
  2. "Complete sequencing and characterization of 21,243 full-length human cDNAs."
    Ota T., Suzuki Y., Nishikawa T., Otsuki T., Sugiyama T., Irie R., Wakamatsu A., Hayashi K., Sato H., Nagai K., Kimura K., Makita H., Sekine M., Obayashi M., Nishi T., Shibahara T., Tanaka T., Ishii S.
    , Yamamoto J., Saito K., Kawai Y., Isono Y., Nakamura Y., Nagahari K., Murakami K., Yasuda T., Iwayanagi T., Wagatsuma M., Shiratori A., Sudo H., Hosoiri T., Kaku Y., Kodaira H., Kondo H., Sugawara M., Takahashi M., Kanda K., Yokoi T., Furuya T., Kikkawa E., Omura Y., Abe K., Kamihara K., Katsuta N., Sato K., Tanikawa M., Yamazaki M., Ninomiya K., Ishibashi T., Yamashita H., Murakawa K., Fujimori K., Tanai H., Kimata M., Watanabe M., Hiraoka S., Chiba Y., Ishida S., Ono Y., Takiguchi S., Watanabe S., Yosida M., Hotuta T., Kusano J., Kanehori K., Takahashi-Fujii A., Hara H., Tanase T.-O., Nomura Y., Togiya S., Komai F., Hara R., Takeuchi K., Arita M., Imose N., Musashino K., Yuuki H., Oshima A., Sasaki N., Aotsuka S., Yoshikawa Y., Matsunawa H., Ichihara T., Shiohata N., Sano S., Moriya S., Momiyama H., Satoh N., Takami S., Terashima Y., Suzuki O., Nakagawa S., Senoh A., Mizoguchi H., Goto Y., Shimizu F., Wakebe H., Hishigaki H., Watanabe T., Sugiyama A., Takemoto M., Kawakami B., Yamazaki M., Watanabe K., Kumagai A., Itakura S., Fukuzumi Y., Fujimori Y., Komiyama M., Tashiro H., Tanigami A., Fujiwara T., Ono T., Yamada K., Fujii Y., Ozaki K., Hirao M., Ohmori Y., Kawabata A., Hikiji T., Kobatake N., Inagaki H., Ikema Y., Okamoto S., Okitani R., Kawakami T., Noguchi S., Itoh T., Shigeta K., Senba T., Matsumura K., Nakajima Y., Mizuno T., Morinaga M., Sasaki M., Togashi T., Oyama M., Hata H., Watanabe M., Komatsu T., Mizushima-Sugano J., Satoh T., Shirai Y., Takahashi Y., Nakagawa K., Okumura K., Nagase T., Nomura N., Kikuchi H., Masuho Y., Yamashita R., Nakai K., Yada T., Nakamura Y., Ohara O., Isogai T., Sugano S.
    Nat. Genet. 36:40-45(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Tissue: Teratocarcinoma.
  3. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
  4. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA], VARIANT VAL-107.
    Tissue: Brain.
  5. "A probability-based approach for high-throughput protein phosphorylation analysis and site localization."
    Beausoleil S.A., Villen J., Gerber S.A., Rush J., Gygi S.P.
    Nat. Biotechnol. 24:1285-1292(2006) [PubMed] [Europe PMC] [Abstract]
    Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-200, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    Tissue: Cervix carcinoma.
  6. "Kinase-selective enrichment enables quantitative phosphoproteomics of the kinome across the cell cycle."
    Daub H., Olsen J.V., Bairlein M., Gnad F., Oppermann F.S., Korner R., Greff Z., Keri G., Stemmann O., Mann M.
    Mol. Cell 31:438-448(2008) [PubMed] [Europe PMC] [Abstract]
    Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-200, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    Tissue: Cervix carcinoma.
  7. Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    Tissue: Cervix carcinoma.
  8. "A functional proteomics approach links the ubiquitin-related modifier Urm1 to a tRNA modification pathway."
    Schlieker C.D., Van der Veen A.G., Damon J.R., Spooner E., Ploegh H.L.
    Proc. Natl. Acad. Sci. U.S.A. 105:18255-18260(2008) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION IN 2-THIOLATION OF TRNA, IDENTIFICATION IN A COMPLEX WITH URM1 AND CTU2.
  9. "Quantitative phosphoproteomics reveals widespread full phosphorylation site occupancy during mitosis."
    Olsen J.V., Vermeulen M., Santamaria A., Kumar C., Miller M.L., Jensen L.J., Gnad F., Cox J., Jensen T.S., Nigg E.A., Brunak S., Mann M.
    Sci. Signal. 3:RA3-RA3(2010) [PubMed] [Europe PMC] [Abstract]
    Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-200, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    Tissue: Cervix carcinoma.

Entry informationi

Entry nameiCTU1_HUMAN
AccessioniPrimary (citable) accession number: Q7Z7A3
Secondary accession number(s): A8K558, Q96GZ7
Entry historyi
Integrated into UniProtKB/Swiss-Prot: April 3, 2007
Last sequence update: October 1, 2003
Last modified: July 9, 2014
This is version 88 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Human chromosome 19
    Human chromosome 19: entries, gene names and cross-references to MIM
  2. Human entries with polymorphisms or disease mutations
    List of human entries with polymorphisms or disease mutations
  3. Human polymorphisms and disease mutations
    Index of human polymorphisms and disease mutations
  4. MIM cross-references
    Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
  5. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  6. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

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