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Q7Z7A3 (CTU1_HUMAN) Reviewed, UniProtKB/Swiss-Prot

Last modified July 9, 2014. Version 88. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (6) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Cytoplasmic tRNA 2-thiolation protein 1

EC=2.7.7.-
Alternative name(s):
ATP-binding domain-containing protein 3
Cancer-associated gene protein
Cytoplasmic tRNA adenylyltransferase 1
Gene names
Name:CTU1
Synonyms:ATPBD3, NCS6
OrganismHomo sapiens (Human) [Reference proteome]
Taxonomic identifier9606 [NCBI]
Taxonomic lineageEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo

Protein attributes

Sequence length348 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Plays a central role in 2-thiolation of mcm5S2U at tRNA wobble positions of tRNA(Lys), tRNA(Glu) and tRNA(Gln). Directly binds tRNAs and probably acts by catalyzing adenylation of tRNAs, an intermediate required for 2-thiolation. It is unclear whether it acts as a sulfurtransferase that transfers sulfur from thiocarboxylated URM1 onto the uridine of tRNAs at wobble position. Ref.8

Pathway

tRNA modification; 5-methoxycarbonylmethyl-2-thiouridine-tRNA biosynthesis. HAMAP-Rule MF_03053

Subunit structure

Component of a complex at least composed of URM1, CTU2/NCS2 and CTU1/ATPBD3. May form a heterodimer with CTU2/NCS2. Ref.8

Subcellular location

Cytoplasm HAMAP-Rule MF_03053.

Sequence similarities

Belongs to the TtcA family. CTU1/NCS6/ATPBD3 subfamily.

Ontologies

Keywords
   Biological processtRNA processing
   Cellular componentCytoplasm
   Coding sequence diversityPolymorphism
   LigandRNA-binding
tRNA-binding
   Molecular functionTransferase
   PTMPhosphoprotein
   Technical termComplete proteome
Reference proteome
Gene Ontology (GO)
   Biological_processprotein urmylation

Inferred from electronic annotation. Source: UniProtKB-HAMAP

tRNA thio-modification

Non-traceable author statement Ref.8. Source: UniProtKB

tRNA wobble uridine modification

Non-traceable author statement Ref.8. Source: UniProtKB

   Cellular_componentcytosol

Inferred from sequence or structural similarity. Source: UniProtKB

mitochondrion

Inferred from electronic annotation. Source: Ensembl

   Molecular_functionnucleotidyltransferase activity

Inferred from electronic annotation. Source: UniProtKB-HAMAP

protein binding

Inferred from physical interaction Ref.8. Source: UniProtKB

tRNA binding

Inferred from electronic annotation. Source: UniProtKB-HAMAP

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 348348Cytoplasmic tRNA 2-thiolation protein 1 HAMAP-Rule MF_03053
PRO_0000282391

Amino acid modifications

Modified residue2001Phosphoserine Ref.5 Ref.6 Ref.9

Natural variations

Natural variant1071A → V. Ref.4
Corresponds to variant rs17855403 [ dbSNP | Ensembl ].
VAR_031402

Experimental info

Sequence conflict711L → R in AAH09037. Ref.4

Sequences

Sequence LengthMass (Da)Tools
Q7Z7A3 [UniParc].

Last modified October 1, 2003. Version 1.
Checksum: 2BA258902B6CA142

FASTA34836,450
        10         20         30         40         50         60 
MPAPPCASCH AARAALRRPL SGQALCGACF CAAFEAEVLH TVLAGRLLPP GAVVAVGASG 

        70         80         90        100        110        120 
GKDSTVLAHV LRALAPRLGI SLQLVAVDEG IGGYRDAALA AVRRQAARWE LPLTVVAYED 

       130        140        150        160        170        180 
LFGGWTMDAV ARSTAGSGRS RSCCTFCGVL RRRALEEGAR RVGATHIVTG HNADDMAETV 

       190        200        210        220        230        240 
LMNFLRGDAG RLARGGGLGS PGEGGALPRC RPLQFASQKE VVLYAHFRRL DYFSEECVYA 

       250        260        270        280        290        300 
PEAFRGHARD LLKRLEAARP SAVLDLVHSA ERLALAPAAR PPRPGACSRC GALASRALCQ 

       310        320        330        340 
ACALLDGLNR GRPRLAIGKG RRGLDEEATP GTPGDPARPP ASKAVPTF 

« Hide

References

« Hide 'large scale' references
[1]"Molecular characterization of a novel gene differentially expressed in breast and prostate cancers."
Yousef G.M., Borgono C.A., Davidian C.T., Michael I., Diamandis E.P.
Submitted (APR-2003) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[2]"Complete sequencing and characterization of 21,243 full-length human cDNAs."
Ota T., Suzuki Y., Nishikawa T., Otsuki T., Sugiyama T., Irie R., Wakamatsu A., Hayashi K., Sato H., Nagai K., Kimura K., Makita H., Sekine M., Obayashi M., Nishi T., Shibahara T., Tanaka T., Ishii S. expand/collapse author list , Yamamoto J., Saito K., Kawai Y., Isono Y., Nakamura Y., Nagahari K., Murakami K., Yasuda T., Iwayanagi T., Wagatsuma M., Shiratori A., Sudo H., Hosoiri T., Kaku Y., Kodaira H., Kondo H., Sugawara M., Takahashi M., Kanda K., Yokoi T., Furuya T., Kikkawa E., Omura Y., Abe K., Kamihara K., Katsuta N., Sato K., Tanikawa M., Yamazaki M., Ninomiya K., Ishibashi T., Yamashita H., Murakawa K., Fujimori K., Tanai H., Kimata M., Watanabe M., Hiraoka S., Chiba Y., Ishida S., Ono Y., Takiguchi S., Watanabe S., Yosida M., Hotuta T., Kusano J., Kanehori K., Takahashi-Fujii A., Hara H., Tanase T.-O., Nomura Y., Togiya S., Komai F., Hara R., Takeuchi K., Arita M., Imose N., Musashino K., Yuuki H., Oshima A., Sasaki N., Aotsuka S., Yoshikawa Y., Matsunawa H., Ichihara T., Shiohata N., Sano S., Moriya S., Momiyama H., Satoh N., Takami S., Terashima Y., Suzuki O., Nakagawa S., Senoh A., Mizoguchi H., Goto Y., Shimizu F., Wakebe H., Hishigaki H., Watanabe T., Sugiyama A., Takemoto M., Kawakami B., Yamazaki M., Watanabe K., Kumagai A., Itakura S., Fukuzumi Y., Fujimori Y., Komiyama M., Tashiro H., Tanigami A., Fujiwara T., Ono T., Yamada K., Fujii Y., Ozaki K., Hirao M., Ohmori Y., Kawabata A., Hikiji T., Kobatake N., Inagaki H., Ikema Y., Okamoto S., Okitani R., Kawakami T., Noguchi S., Itoh T., Shigeta K., Senba T., Matsumura K., Nakajima Y., Mizuno T., Morinaga M., Sasaki M., Togashi T., Oyama M., Hata H., Watanabe M., Komatsu T., Mizushima-Sugano J., Satoh T., Shirai Y., Takahashi Y., Nakagawa K., Okumura K., Nagase T., Nomura N., Kikuchi H., Masuho Y., Yamashita R., Nakai K., Yada T., Nakamura Y., Ohara O., Isogai T., Sugano S.
Nat. Genet. 36:40-45(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
Tissue: Teratocarcinoma.
[3]Mural R.J., Istrail S., Sutton G.G., Florea L., Halpern A.L., Mobarry C.M., Lippert R., Walenz B., Shatkay H., Dew I., Miller J.R., Flanigan M.J., Edwards N.J., Bolanos R., Fasulo D., Halldorsson B.V., Hannenhalli S., Turner R. expand/collapse author list , Yooseph S., Lu F., Nusskern D.R., Shue B.C., Zheng X.H., Zhong F., Delcher A.L., Huson D.H., Kravitz S.A., Mouchard L., Reinert K., Remington K.A., Clark A.G., Waterman M.S., Eichler E.E., Adams M.D., Hunkapiller M.W., Myers E.W., Venter J.C.
Submitted (JUL-2005) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[4]"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
The MGC Project Team
Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA], VARIANT VAL-107.
Tissue: Brain.
[5]"A probability-based approach for high-throughput protein phosphorylation analysis and site localization."
Beausoleil S.A., Villen J., Gerber S.A., Rush J., Gygi S.P.
Nat. Biotechnol. 24:1285-1292(2006) [PubMed] [Europe PMC] [Abstract]
Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-200, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
Tissue: Cervix carcinoma.
[6]"Kinase-selective enrichment enables quantitative phosphoproteomics of the kinome across the cell cycle."
Daub H., Olsen J.V., Bairlein M., Gnad F., Oppermann F.S., Korner R., Greff Z., Keri G., Stemmann O., Mann M.
Mol. Cell 31:438-448(2008) [PubMed] [Europe PMC] [Abstract]
Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-200, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
Tissue: Cervix carcinoma.
[7]"A quantitative atlas of mitotic phosphorylation."
Dephoure N., Zhou C., Villen J., Beausoleil S.A., Bakalarski C.E., Elledge S.J., Gygi S.P.
Proc. Natl. Acad. Sci. U.S.A. 105:10762-10767(2008) [PubMed] [Europe PMC] [Abstract]
Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
Tissue: Cervix carcinoma.
[8]"A functional proteomics approach links the ubiquitin-related modifier Urm1 to a tRNA modification pathway."
Schlieker C.D., Van der Veen A.G., Damon J.R., Spooner E., Ploegh H.L.
Proc. Natl. Acad. Sci. U.S.A. 105:18255-18260(2008) [PubMed] [Europe PMC] [Abstract]
Cited for: FUNCTION IN 2-THIOLATION OF TRNA, IDENTIFICATION IN A COMPLEX WITH URM1 AND CTU2.
[9]"Quantitative phosphoproteomics reveals widespread full phosphorylation site occupancy during mitosis."
Olsen J.V., Vermeulen M., Santamaria A., Kumar C., Miller M.L., Jensen L.J., Gnad F., Cox J., Jensen T.S., Nigg E.A., Brunak S., Mann M.
Sci. Signal. 3:RA3-RA3(2010) [PubMed] [Europe PMC] [Abstract]
Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-200, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
Tissue: Cervix carcinoma.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AY279382 Genomic DNA. Translation: AAP42277.1.
AK291173 mRNA. Translation: BAF83862.1.
CH471135 Genomic DNA. Translation: EAW71984.1.
BC009037 mRNA. Translation: AAH09037.1.
CCDSCCDS12824.1.
RefSeqNP_660275.2. NM_145232.3.
UniGeneHs.148425.

3D structure databases

ProteinModelPortalQ7Z7A3.
SMRQ7Z7A3. Positions 46-254.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

BioGrid124701. 5 interactions.
DIPDIP-48632N.
STRING9606.ENSP00000270637.

PTM databases

PhosphoSiteQ7Z7A3.

Polymorphism databases

DMDM74713747.

Proteomic databases

MaxQBQ7Z7A3.
PaxDbQ7Z7A3.
PRIDEQ7Z7A3.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblENST00000421832; ENSP00000390011; ENSG00000142544.
GeneID90353.
KEGGhsa:90353.
UCSCuc010eop.3. human.

Organism-specific databases

CTD90353.
GeneCardsGC19M051601.
H-InvDBHIX0015377.
HIX0137480.
HGNCHGNC:29590. CTU1.
HPAHPA053797.
MIM612694. gene.
neXtProtNX_Q7Z7A3.
PharmGKBPA165393368.
GenAtlasSearch...

Phylogenomic databases

eggNOGCOG0037.
HOGENOMHOG000225864.
HOVERGENHBG100428.
InParanoidQ7Z7A3.
KOK14168.
OMAICTQGED.
OrthoDBEOG7PGDRH.
PhylomeDBQ7Z7A3.
TreeFamTF352405.

Enzyme and pathway databases

UniPathwayUPA00988.

Gene expression databases

BgeeQ7Z7A3.
CleanExHS_ATPBD3.
GenevestigatorQ7Z7A3.

Family and domain databases

Gene3D3.40.50.620. 1 hit.
HAMAPMF_03053. CTU1.
InterProIPR012089. 2-thiocytidine_tRNA_synth_TtcA.
IPR000541. Ncs6/Tuc1/Ctu1.
IPR014729. Rossmann-like_a/b/a_fold.
IPR011063. tRNA-lysidine/thiocyt_synth.
[Graphical view]
PfamPF01171. ATP_bind_3. 1 hit.
[Graphical view]
PIRSFPIRSF004976. ATPase_YdaO. 1 hit.
ProtoNetSearch...

Other

GenomeRNAi90353.
NextBio76697.
PROQ7Z7A3.
SOURCESearch...

Entry information

Entry nameCTU1_HUMAN
AccessionPrimary (citable) accession number: Q7Z7A3
Secondary accession number(s): A8K558, Q96GZ7
Entry history
Integrated into UniProtKB/Swiss-Prot: April 3, 2007
Last sequence update: October 1, 2003
Last modified: July 9, 2014
This is version 88 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Relevant documents

SIMILARITY comments

Index of protein domains and families

PATHWAY comments

Index of metabolic and biosynthesis pathways

MIM cross-references

Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot

Human polymorphisms and disease mutations

Index of human polymorphisms and disease mutations

Human entries with polymorphisms or disease mutations

List of human entries with polymorphisms or disease mutations

Human chromosome 19

Human chromosome 19: entries, gene names and cross-references to MIM