Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Basket 0
(max 400 entries)x

Your basket is currently empty.

Select item(s) and click on "Add to basket" to create your own collection here
(400 entries max)

Q7Z7A3

- CTU1_HUMAN

UniProt

Q7Z7A3 - CTU1_HUMAN

Protein

Cytoplasmic tRNA 2-thiolation protein 1

Gene

CTU1

Organism
Homo sapiens (Human)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli
    • BLAST
    • Align
    • Format
    • Add to basket
    • History
      Entry version 89 (01 Oct 2014)
      Sequence version 1 (01 Oct 2003)
      Previous versions | rss
    • Help video
    • Feedback
    • Comment

    Functioni

    Plays a central role in 2-thiolation of mcm5S2U at tRNA wobble positions of tRNA(Lys), tRNA(Glu) and tRNA(Gln). Directly binds tRNAs and probably acts by catalyzing adenylation of tRNAs, an intermediate required for 2-thiolation. It is unclear whether it acts as a sulfurtransferase that transfers sulfur from thiocarboxylated URM1 onto the uridine of tRNAs at wobble position.1 PublicationUniRule annotation

    Pathwayi

    GO - Molecular functioni

    1. nucleotidyltransferase activity Source: UniProtKB-HAMAP
    2. protein binding Source: UniProtKB
    3. tRNA binding Source: UniProtKB-HAMAP

    GO - Biological processi

    1. protein urmylation Source: UniProtKB-HAMAP
    2. tRNA thio-modification Source: UniProtKB
    3. tRNA wobble uridine modification Source: UniProtKB

    Keywords - Molecular functioni

    Transferase

    Keywords - Biological processi

    tRNA processing

    Keywords - Ligandi

    RNA-binding, tRNA-binding

    Enzyme and pathway databases

    UniPathwayiUPA00988.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Cytoplasmic tRNA 2-thiolation protein 1UniRule annotation (EC:2.7.7.-UniRule annotation)
    Alternative name(s):
    ATP-binding domain-containing protein 3UniRule annotation
    Cancer-associated gene protein
    Cytoplasmic tRNA adenylyltransferase 1UniRule annotation
    Gene namesi
    Name:CTU1UniRule annotation
    Synonyms:ATPBD3UniRule annotation, NCS6UniRule annotation
    OrganismiHomo sapiens (Human)
    Taxonomic identifieri9606 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
    ProteomesiUP000005640: Chromosome 19

    Organism-specific databases

    HGNCiHGNC:29590. CTU1.

    Subcellular locationi

    GO - Cellular componenti

    1. cytosol Source: UniProtKB
    2. mitochondrion Source: Ensembl

    Keywords - Cellular componenti

    Cytoplasm

    Pathology & Biotechi

    Organism-specific databases

    PharmGKBiPA165393368.

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 348348Cytoplasmic tRNA 2-thiolation protein 1PRO_0000282391Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Modified residuei200 – 2001Phosphoserine3 Publications

    Keywords - PTMi

    Phosphoprotein

    Proteomic databases

    MaxQBiQ7Z7A3.
    PaxDbiQ7Z7A3.
    PRIDEiQ7Z7A3.

    PTM databases

    PhosphoSiteiQ7Z7A3.

    Expressioni

    Gene expression databases

    BgeeiQ7Z7A3.
    CleanExiHS_ATPBD3.
    GenevestigatoriQ7Z7A3.

    Organism-specific databases

    HPAiHPA053797.

    Interactioni

    Subunit structurei

    Component of a complex at least composed of URM1, CTU2/NCS2 and CTU1/ATPBD3. May form a heterodimer with CTU2/NCS2.1 PublicationUniRule annotation

    Protein-protein interaction databases

    BioGridi124701. 5 interactions.
    DIPiDIP-48632N.
    STRINGi9606.ENSP00000270637.

    Structurei

    3D structure databases

    ProteinModelPortaliQ7Z7A3.
    SMRiQ7Z7A3. Positions 46-254.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Sequence similaritiesi

    Belongs to the TtcA family. CTU1/NCS6/ATPBD3 subfamily.UniRule annotation

    Phylogenomic databases

    eggNOGiCOG0037.
    HOGENOMiHOG000225864.
    HOVERGENiHBG100428.
    InParanoidiQ7Z7A3.
    KOiK14168.
    OMAiICTQGED.
    OrthoDBiEOG7PGDRH.
    PhylomeDBiQ7Z7A3.
    TreeFamiTF352405.

    Family and domain databases

    Gene3Di3.40.50.620. 1 hit.
    HAMAPiMF_03053. CTU1.
    InterProiIPR012089. 2-thiocytidine_tRNA_synth_TtcA.
    IPR000541. Ncs6/Tuc1/Ctu1.
    IPR014729. Rossmann-like_a/b/a_fold.
    IPR011063. tRNA-lysidine/thiocyt_synth.
    [Graphical view]
    PfamiPF01171. ATP_bind_3. 1 hit.
    [Graphical view]
    PIRSFiPIRSF004976. ATPase_YdaO. 1 hit.

    Sequencei

    Sequence statusi: Complete.

    Q7Z7A3-1 [UniParc]FASTAAdd to Basket

    « Hide

    MPAPPCASCH AARAALRRPL SGQALCGACF CAAFEAEVLH TVLAGRLLPP    50
    GAVVAVGASG GKDSTVLAHV LRALAPRLGI SLQLVAVDEG IGGYRDAALA 100
    AVRRQAARWE LPLTVVAYED LFGGWTMDAV ARSTAGSGRS RSCCTFCGVL 150
    RRRALEEGAR RVGATHIVTG HNADDMAETV LMNFLRGDAG RLARGGGLGS 200
    PGEGGALPRC RPLQFASQKE VVLYAHFRRL DYFSEECVYA PEAFRGHARD 250
    LLKRLEAARP SAVLDLVHSA ERLALAPAAR PPRPGACSRC GALASRALCQ 300
    ACALLDGLNR GRPRLAIGKG RRGLDEEATP GTPGDPARPP ASKAVPTF 348
    Length:348
    Mass (Da):36,450
    Last modified:October 1, 2003 - v1
    Checksum:i2BA258902B6CA142
    GO

    Experimental Info

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sequence conflicti71 – 711L → R in AAH09037. (PubMed:15489334)Curated

    Natural variant

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Natural varianti107 – 1071A → V.1 Publication
    Corresponds to variant rs17855403 [ dbSNP | Ensembl ].
    VAR_031402

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AY279382 Genomic DNA. Translation: AAP42277.1.
    AK291173 mRNA. Translation: BAF83862.1.
    CH471135 Genomic DNA. Translation: EAW71984.1.
    BC009037 mRNA. Translation: AAH09037.1.
    CCDSiCCDS12824.1.
    RefSeqiNP_660275.2. NM_145232.3.
    UniGeneiHs.148425.

    Genome annotation databases

    EnsembliENST00000421832; ENSP00000390011; ENSG00000142544.
    GeneIDi90353.
    KEGGihsa:90353.
    UCSCiuc010eop.3. human.

    Polymorphism databases

    DMDMi74713747.

    Keywords - Coding sequence diversityi

    Polymorphism

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AY279382 Genomic DNA. Translation: AAP42277.1 .
    AK291173 mRNA. Translation: BAF83862.1 .
    CH471135 Genomic DNA. Translation: EAW71984.1 .
    BC009037 mRNA. Translation: AAH09037.1 .
    CCDSi CCDS12824.1.
    RefSeqi NP_660275.2. NM_145232.3.
    UniGenei Hs.148425.

    3D structure databases

    ProteinModelPortali Q7Z7A3.
    SMRi Q7Z7A3. Positions 46-254.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    BioGridi 124701. 5 interactions.
    DIPi DIP-48632N.
    STRINGi 9606.ENSP00000270637.

    PTM databases

    PhosphoSitei Q7Z7A3.

    Polymorphism databases

    DMDMi 74713747.

    Proteomic databases

    MaxQBi Q7Z7A3.
    PaxDbi Q7Z7A3.
    PRIDEi Q7Z7A3.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    Ensembli ENST00000421832 ; ENSP00000390011 ; ENSG00000142544 .
    GeneIDi 90353.
    KEGGi hsa:90353.
    UCSCi uc010eop.3. human.

    Organism-specific databases

    CTDi 90353.
    GeneCardsi GC19M051601.
    H-InvDB HIX0015377.
    HIX0137480.
    HGNCi HGNC:29590. CTU1.
    HPAi HPA053797.
    MIMi 612694. gene.
    neXtProti NX_Q7Z7A3.
    PharmGKBi PA165393368.
    GenAtlasi Search...

    Phylogenomic databases

    eggNOGi COG0037.
    HOGENOMi HOG000225864.
    HOVERGENi HBG100428.
    InParanoidi Q7Z7A3.
    KOi K14168.
    OMAi ICTQGED.
    OrthoDBi EOG7PGDRH.
    PhylomeDBi Q7Z7A3.
    TreeFami TF352405.

    Enzyme and pathway databases

    UniPathwayi UPA00988 .

    Miscellaneous databases

    GenomeRNAii 90353.
    NextBioi 76697.
    PROi Q7Z7A3.
    SOURCEi Search...

    Gene expression databases

    Bgeei Q7Z7A3.
    CleanExi HS_ATPBD3.
    Genevestigatori Q7Z7A3.

    Family and domain databases

    Gene3Di 3.40.50.620. 1 hit.
    HAMAPi MF_03053. CTU1.
    InterProi IPR012089. 2-thiocytidine_tRNA_synth_TtcA.
    IPR000541. Ncs6/Tuc1/Ctu1.
    IPR014729. Rossmann-like_a/b/a_fold.
    IPR011063. tRNA-lysidine/thiocyt_synth.
    [Graphical view ]
    Pfami PF01171. ATP_bind_3. 1 hit.
    [Graphical view ]
    PIRSFi PIRSF004976. ATPase_YdaO. 1 hit.
    ProtoNeti Search...

    Publicationsi

    1. "Molecular characterization of a novel gene differentially expressed in breast and prostate cancers."
      Yousef G.M., Borgono C.A., Davidian C.T., Michael I., Diamandis E.P.
      Submitted (APR-2003) to the EMBL/GenBank/DDBJ databases
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    2. "Complete sequencing and characterization of 21,243 full-length human cDNAs."
      Ota T., Suzuki Y., Nishikawa T., Otsuki T., Sugiyama T., Irie R., Wakamatsu A., Hayashi K., Sato H., Nagai K., Kimura K., Makita H., Sekine M., Obayashi M., Nishi T., Shibahara T., Tanaka T., Ishii S.
      , Yamamoto J., Saito K., Kawai Y., Isono Y., Nakamura Y., Nagahari K., Murakami K., Yasuda T., Iwayanagi T., Wagatsuma M., Shiratori A., Sudo H., Hosoiri T., Kaku Y., Kodaira H., Kondo H., Sugawara M., Takahashi M., Kanda K., Yokoi T., Furuya T., Kikkawa E., Omura Y., Abe K., Kamihara K., Katsuta N., Sato K., Tanikawa M., Yamazaki M., Ninomiya K., Ishibashi T., Yamashita H., Murakawa K., Fujimori K., Tanai H., Kimata M., Watanabe M., Hiraoka S., Chiba Y., Ishida S., Ono Y., Takiguchi S., Watanabe S., Yosida M., Hotuta T., Kusano J., Kanehori K., Takahashi-Fujii A., Hara H., Tanase T.-O., Nomura Y., Togiya S., Komai F., Hara R., Takeuchi K., Arita M., Imose N., Musashino K., Yuuki H., Oshima A., Sasaki N., Aotsuka S., Yoshikawa Y., Matsunawa H., Ichihara T., Shiohata N., Sano S., Moriya S., Momiyama H., Satoh N., Takami S., Terashima Y., Suzuki O., Nakagawa S., Senoh A., Mizoguchi H., Goto Y., Shimizu F., Wakebe H., Hishigaki H., Watanabe T., Sugiyama A., Takemoto M., Kawakami B., Yamazaki M., Watanabe K., Kumagai A., Itakura S., Fukuzumi Y., Fujimori Y., Komiyama M., Tashiro H., Tanigami A., Fujiwara T., Ono T., Yamada K., Fujii Y., Ozaki K., Hirao M., Ohmori Y., Kawabata A., Hikiji T., Kobatake N., Inagaki H., Ikema Y., Okamoto S., Okitani R., Kawakami T., Noguchi S., Itoh T., Shigeta K., Senba T., Matsumura K., Nakajima Y., Mizuno T., Morinaga M., Sasaki M., Togashi T., Oyama M., Hata H., Watanabe M., Komatsu T., Mizushima-Sugano J., Satoh T., Shirai Y., Takahashi Y., Nakagawa K., Okumura K., Nagase T., Nomura N., Kikuchi H., Masuho Y., Yamashita R., Nakai K., Yada T., Nakamura Y., Ohara O., Isogai T., Sugano S.
      Nat. Genet. 36:40-45(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
      Tissue: Teratocarcinoma.
    3. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    4. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
      The MGC Project Team
      Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA], VARIANT VAL-107.
      Tissue: Brain.
    5. "A probability-based approach for high-throughput protein phosphorylation analysis and site localization."
      Beausoleil S.A., Villen J., Gerber S.A., Rush J., Gygi S.P.
      Nat. Biotechnol. 24:1285-1292(2006) [PubMed] [Europe PMC] [Abstract]
      Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-200, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
      Tissue: Cervix carcinoma.
    6. "Kinase-selective enrichment enables quantitative phosphoproteomics of the kinome across the cell cycle."
      Daub H., Olsen J.V., Bairlein M., Gnad F., Oppermann F.S., Korner R., Greff Z., Keri G., Stemmann O., Mann M.
      Mol. Cell 31:438-448(2008) [PubMed] [Europe PMC] [Abstract]
      Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-200, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
      Tissue: Cervix carcinoma.
    7. Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
      Tissue: Cervix carcinoma.
    8. "A functional proteomics approach links the ubiquitin-related modifier Urm1 to a tRNA modification pathway."
      Schlieker C.D., Van der Veen A.G., Damon J.R., Spooner E., Ploegh H.L.
      Proc. Natl. Acad. Sci. U.S.A. 105:18255-18260(2008) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION IN 2-THIOLATION OF TRNA, IDENTIFICATION IN A COMPLEX WITH URM1 AND CTU2.
    9. "Quantitative phosphoproteomics reveals widespread full phosphorylation site occupancy during mitosis."
      Olsen J.V., Vermeulen M., Santamaria A., Kumar C., Miller M.L., Jensen L.J., Gnad F., Cox J., Jensen T.S., Nigg E.A., Brunak S., Mann M.
      Sci. Signal. 3:RA3-RA3(2010) [PubMed] [Europe PMC] [Abstract]
      Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-200, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
      Tissue: Cervix carcinoma.

    Entry informationi

    Entry nameiCTU1_HUMAN
    AccessioniPrimary (citable) accession number: Q7Z7A3
    Secondary accession number(s): A8K558, Q96GZ7
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: April 3, 2007
    Last sequence update: October 1, 2003
    Last modified: October 1, 2014
    This is version 89 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program
    DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. Human chromosome 19
      Human chromosome 19: entries, gene names and cross-references to MIM
    2. Human entries with polymorphisms or disease mutations
      List of human entries with polymorphisms or disease mutations
    3. Human polymorphisms and disease mutations
      Index of human polymorphisms and disease mutations
    4. MIM cross-references
      Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
    5. PATHWAY comments
      Index of metabolic and biosynthesis pathways
    6. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3