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Protein

Cytoplasmic tRNA 2-thiolation protein 1

Gene

CTU1

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Plays a central role in 2-thiolation of mcm5S2U at tRNA wobble positions of tRNA(Lys), tRNA(Glu) and tRNA(Gln). Directly binds tRNAs and probably acts by catalyzing adenylation of tRNAs, an intermediate required for 2-thiolation. It is unclear whether it acts as a sulfurtransferase that transfers sulfur from thiocarboxylated URM1 onto the uridine of tRNAs at wobble position.UniRule annotation1 Publication

Pathwayi: 5-methoxycarbonylmethyl-2-thiouridine-tRNA biosynthesis

This protein is involved in the pathway 5-methoxycarbonylmethyl-2-thiouridine-tRNA biosynthesis, which is part of tRNA modification.UniRule annotation
View all proteins of this organism that are known to be involved in the pathway 5-methoxycarbonylmethyl-2-thiouridine-tRNA biosynthesis and in tRNA modification.

GO - Molecular functioni

GO - Biological processi

  • tRNA thio-modification Source: UniProtKB
  • tRNA wobble uridine modification Source: UniProtKB

Keywordsi

Molecular functionRNA-binding, Transferase, tRNA-binding
Biological processtRNA processing

Enzyme and pathway databases

BioCyciMetaCyc:ENSG00000142544-MONOMER
ReactomeiR-HSA-6782315 tRNA modification in the nucleus and cytosol
UniPathwayiUPA00988

Names & Taxonomyi

Protein namesi
Recommended name:
Cytoplasmic tRNA 2-thiolation protein 1UniRule annotation (EC:2.7.7.-UniRule annotation)
Alternative name(s):
ATP-binding domain-containing protein 3UniRule annotation
Cancer-associated gene protein
Cytoplasmic tRNA adenylyltransferase 1UniRule annotation
Gene namesi
Name:CTU1UniRule annotation
Synonyms:ATPBD3UniRule annotation, NCS6UniRule annotation
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
Proteomesi
  • UP000005640 Componenti: Chromosome 19

Organism-specific databases

EuPathDBiHostDB:ENSG00000142544.6
HGNCiHGNC:29590 CTU1
MIMi612694 gene
neXtProtiNX_Q7Z7A3

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Cytoplasm

Pathology & Biotechi

Organism-specific databases

DisGeNETi90353
OpenTargetsiENSG00000142544
PharmGKBiPA165393368

Polymorphism and mutation databases

BioMutaiCTU1
DMDMi74713747

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00002823911 – 348Cytoplasmic tRNA 2-thiolation protein 1Add BLAST348

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei200PhosphoserineCombined sources1

Keywords - PTMi

Phosphoprotein

Proteomic databases

EPDiQ7Z7A3
MaxQBiQ7Z7A3
PaxDbiQ7Z7A3
PeptideAtlasiQ7Z7A3
PRIDEiQ7Z7A3

PTM databases

iPTMnetiQ7Z7A3
PhosphoSitePlusiQ7Z7A3

Expressioni

Gene expression databases

BgeeiENSG00000142544
CleanExiHS_ATPBD3

Organism-specific databases

HPAiHPA053797

Interactioni

Subunit structurei

Component of a complex at least composed of URM1, CTU2/NCS2 and CTU1/ATPBD3. May form a heterodimer with CTU2/NCS2.UniRule annotation1 Publication

Binary interactionsi

WithEntry#Exp.IntActNotes
URM1Q9BTM97EBI-15741880,EBI-714589

Protein-protein interaction databases

BioGridi124701, 33 interactors
DIPiDIP-48632N
IntActiQ7Z7A3, 3 interactors
STRINGi9606.ENSP00000390011

Structurei

3D structure databases

ProteinModelPortaliQ7Z7A3
SMRiQ7Z7A3
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the TtcA family. CTU1/NCS6/ATPBD3 subfamily.UniRule annotation

Phylogenomic databases

eggNOGiKOG2840 Eukaryota
COG0037 LUCA
GeneTreeiENSGT00390000001041
HOGENOMiHOG000225864
HOVERGENiHBG100428
InParanoidiQ7Z7A3
KOiK14168
OMAiKPLKYSY
OrthoDBiEOG091G0CF7
PhylomeDBiQ7Z7A3
TreeFamiTF352405

Family and domain databases

Gene3Di3.40.50.620, 1 hit
HAMAPiMF_03053 CTU1, 1 hit
InterProiView protein in InterPro
IPR032442 CTU1_C
IPR000541 Ncs6/Tuc1/Ctu1
IPR014729 Rossmann-like_a/b/a_fold
IPR011063 TilS/TtcA_N
IPR035107 tRNA_thiolation_TtcA_Ctu1
PANTHERiPTHR11807:SF12 PTHR11807:SF12, 1 hit
PfamiView protein in Pfam
PF01171 ATP_bind_3, 1 hit
PF16503 zn-ribbon_14, 1 hit
PIRSFiPIRSF004976 ATPase_YdaO, 1 hit

Sequencei

Sequence statusi: Complete.

Q7Z7A3-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MPAPPCASCH AARAALRRPL SGQALCGACF CAAFEAEVLH TVLAGRLLPP
60 70 80 90 100
GAVVAVGASG GKDSTVLAHV LRALAPRLGI SLQLVAVDEG IGGYRDAALA
110 120 130 140 150
AVRRQAARWE LPLTVVAYED LFGGWTMDAV ARSTAGSGRS RSCCTFCGVL
160 170 180 190 200
RRRALEEGAR RVGATHIVTG HNADDMAETV LMNFLRGDAG RLARGGGLGS
210 220 230 240 250
PGEGGALPRC RPLQFASQKE VVLYAHFRRL DYFSEECVYA PEAFRGHARD
260 270 280 290 300
LLKRLEAARP SAVLDLVHSA ERLALAPAAR PPRPGACSRC GALASRALCQ
310 320 330 340
ACALLDGLNR GRPRLAIGKG RRGLDEEATP GTPGDPARPP ASKAVPTF
Length:348
Mass (Da):36,450
Last modified:October 1, 2003 - v1
Checksum:i2BA258902B6CA142
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti71L → R in AAH09037 (PubMed:15489334).Curated1

Natural variant

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Natural variantiVAR_031402107A → V1 PublicationCorresponds to variant dbSNP:rs17855403Ensembl.1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AY279382 Genomic DNA Translation: AAP42277.1
AK291173 mRNA Translation: BAF83862.1
CH471135 Genomic DNA Translation: EAW71984.1
BC009037 mRNA Translation: AAH09037.1
CCDSiCCDS12824.1
RefSeqiNP_660275.2, NM_145232.3
UniGeneiHs.148425

Genome annotation databases

EnsembliENST00000421832; ENSP00000390011; ENSG00000142544
GeneIDi90353
KEGGihsa:90353
UCSCiuc010eop.4 human

Keywords - Coding sequence diversityi

Polymorphism

Similar proteinsi

Entry informationi

Entry nameiCTU1_HUMAN
AccessioniPrimary (citable) accession number: Q7Z7A3
Secondary accession number(s): A8K558, Q96GZ7
Entry historyiIntegrated into UniProtKB/Swiss-Prot: April 3, 2007
Last sequence update: October 1, 2003
Last modified: May 23, 2018
This is version 114 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

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