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Protein

Maestro heat-like repeat-containing protein family member 2B

Gene

MROH2B

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at transcript leveli

Functioni

May play a role in the process of sperm capacitation.By similarity

GO - Biological processi

Keywordsi

Biological processDifferentiation, Spermatogenesis

Names & Taxonomyi

Protein namesi
Recommended name:
Maestro heat-like repeat-containing protein family member 2BImported
Alternative name(s):
HEAT repeat-containing protein 7B2
Sperm PKA-interacting factorBy similarity
Short name:
SPIFBy similarity
Gene namesi
Name:MROH2BImported
Synonyms:HEATR7B2
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
Proteomesi
  • UP000005640 Componenti: Chromosome 5

Organism-specific databases

HGNCiHGNC:26857. MROH2B.

Subcellular locationi

  • Cytoplasm By similarity
  • Cytoplasmic vesiclesecretory vesicleacrosome By similarity
  • Cell projectionciliumflagellum By similarity

  • Note: Colocalizes with PRKACA and TCP11 on the acrosome and tail regions in round spermatids and spermatozoa regardless of the capacitation status of the sperm.By similarity

GO - Cellular componenti

Keywords - Cellular componenti

Cell projection, Cytoplasm, Cytoplasmic vesicle

Pathology & Biotechi

Organism-specific databases

DisGeNETi133558.
OpenTargetsiENSG00000171495.
PharmGKBiPA164720440.

Polymorphism and mutation databases

BioMutaiMROH2B.
DMDMi296453066.

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00003322431 – 1585Maestro heat-like repeat-containing protein family member 2BAdd BLAST1585

Post-translational modificationi

Constitutively phosphorylated on serine and threonine residues in acrosomal region of the sperm head, midpiece and flagellar regions of noncapacitated spermatozoa. Phosphorylation on tyrosine residues increases upon sperm capacitation within the acrosomal and tail regions in a protein kinase A (PKA)-dependent signaling pathway.By similarity

Proteomic databases

EPDiQ7Z745.
PaxDbiQ7Z745.
PeptideAtlasiQ7Z745.
PRIDEiQ7Z745.

PTM databases

iPTMnetiQ7Z745.
PhosphoSitePlusiQ7Z745.

Expressioni

Gene expression databases

BgeeiENSG00000171495.
ExpressionAtlasiQ7Z745. baseline and differential.
GenevisibleiQ7Z745. HS.

Organism-specific databases

HPAiHPA059457.

Interactioni

Subunit structurei

Found in a complex at least composed of MROH2B, PRKACA isoform 2 and TCP11. Interacts with PRKACA. Interacts with TCP11.By similarity

Protein-protein interaction databases

BioGridi126362. 1 interactor.
STRINGi9606.ENSP00000382476.

Structurei

3D structure databases

ProteinModelPortaliQ7Z745.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Repeati28 – 65HEAT 1Add BLAST38
Repeati228 – 263HEAT 2Add BLAST36
Repeati272 – 309HEAT 3Add BLAST38
Repeati310 – 346HEAT 4Add BLAST37
Repeati405 – 445HEAT 5Add BLAST41
Repeati531 – 569HEAT 6Add BLAST39
Repeati572 – 611HEAT 7Add BLAST40
Repeati662 – 699HEAT 8Add BLAST38
Repeati777 – 819HEAT 9Add BLAST43
Repeati964 – 1001HEAT 10Add BLAST38
Repeati1021 – 1059HEAT 11Add BLAST39
Repeati1112 – 1151HEAT 12Add BLAST40
Repeati1157 – 1195HEAT 13Add BLAST39
Repeati1258 – 1295HEAT 14Add BLAST38
Repeati1363 – 1402HEAT 15Add BLAST40

Keywords - Domaini

Repeat

Phylogenomic databases

eggNOGiKOG2032. Eukaryota.
ENOG410XQE8. LUCA.
GeneTreeiENSGT00880000137905.
HOGENOMiHOG000168416.
HOVERGENiHBG108702.
InParanoidiQ7Z745.
OMAiYYASKDM.
OrthoDBiEOG091G00DQ.
PhylomeDBiQ7Z745.
TreeFamiTF315201.

Family and domain databases

Gene3Di1.25.10.10. 3 hits.
InterProiView protein in InterPro
IPR011989. ARM-like.
IPR016024. ARM-type_fold.
SUPFAMiSSF48371. SSF48371. 4 hits.

Sequences (2)i

Sequence statusi: Complete.

This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 1 (identifier: Q7Z745-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MTLSTEESIE MFGDINLTLG MLNKEDIVNK EDIYSHLTSV IQNTDILDDA
60 70 80 90 100
IVQRLIYYAS KDMRDNNMLR EIRMLAGEVL VSLAAHDFNS VMYEVQSNFR
110 120 130 140 150
ILELPDEFVV LALAELATSY VSQSIPFMMM TLLTMQTMLR LAEDERMKGT
160 170 180 190 200
FCIALEKFSK AIYKYVNHWR DFPYPRLDAN RLSDKIFMLF WYIMEKWAPL
210 220 230 240 250
ASPMQTLSIV KAHGPTVSLL LHREDFRGYA LGQVPWLLNQ YKDKEIDFHV
260 270 280 290 300
TQSLKQILTA AVLYDIGLPR SLRRSIFINL LQQICRAPEP PVKENEMKAS
310 320 330 340 350
SCFLILAHSN PGELMEFFDE QVRSNNEAIR VGILTLLRLA VNADEPRLRD
360 370 380 390 400
HIISIERTVK IVMGDLSTKV RNSVLLLIQT MCEKSYIEAR EGWPLIDYVF
410 420 430 440 450
SQFATLNRNL EKPVKTNFHE NEKEEESVRE TSLEVLKTLD PLVIGMPQVL
460 470 480 490 500
WPRILTFVVP AEYTEALEPL FSIIRILIMA EEKKQHSAKE STALVVSTGA
510 520 530 540 550
VKLPSPQQLL ARLLVISMPA SLGELRGAGA IGLLKILPEI IHPKLVDLWK
560 570 580 590 600
TRLPELLQPL EGKNISTVLW ETMLLQLLKE SLWKISDVAW TIQLTQDFKQ
610 620 630 640 650
QMGSYSNNST EKKFLWKALG TTLACCQDSD FVNSQIKEFL TAPNQLGDQR
660 670 680 690 700
QGITSILGYC AENHLDIVLK VLKTFQNQEK FFMNRCKSLF SGKKSLTKTD
710 720 730 740 750
VMVIYGAVAL HAPKKQLLSR LNQDIISQVL SLHGQCSQVL GMSVMNKDMD
760 770 780 790 800
LQMSFTRSIT EIGIAVQDAE DQGFQFSYKE MLIGYMLDFI RDEPLDSLAS
810 820 830 840 850
PIRWKALIAI RYLSKLKPQL SLQDHLNILE ENIRRLLPLP PLENLKSEGQ
860 870 880 890 900
TDKDKEHIQF LYERSMDALG KLLKTMMWDN VNAEDCQEMF NLLQMWLVSQ
910 920 930 940 950
KEWERERAFQ ITAKVLTNDI EAPENFKIGS LLGLLAPHSC DTLPTIRQAA
960 970 980 990 1000
ASSTIGLFYI KGIHLEVERL QGLQEGLESD DVQVQIKISS KIAKIVSKFI
1010 1020 1030 1040 1050
PNEEILMFLE EMLDGLESLN PTCTKACGIW MITVLKQQGA ALEDQLLEIL
1060 1070 1080 1090 1100
GTIYHHMPVL RQKEESFQFI LEAISQIASF HMDTVVVNLL QKPLPFDRDT
1110 1120 1130 1140 1150
KTLWKALAEK PASSGKLLQA LIDKLETELE DDIARVEAIS VACAMYEVIS
1160 1170 1180 1190 1200
MGTSVTGLYP ELFTLLLKLV SCTLGQKMLT CPWSHRRHVM QQGEQQQIPD
1210 1220 1230 1240 1250
PCRLSTATLK CLQAQAMREG LAKESDEGDN LWTLLSSPST HHIGVCSLAR
1260 1270 1280 1290 1300
SMAVWQHGVI LDIMEQLLSS LTSSSENYRI TGAAFFSELM KEPILWKHGN
1310 1320 1330 1340 1350
LRNVLILMDQ SAWDSNATLR QMAIRGLGNT ASGAPHKVKK HKQLMLESII
1360 1370 1380 1390 1400
RGLYHLARTE VVCESLKALK KILELLTDRD VSFYFKEIVL QTRTFFEDEQ
1410 1420 1430 1440 1450
DDVRLTAIFL FEDLAPLTGR RWKIFFAEEI KKSLISFLLH LWDPNPKIGV
1460 1470 1480 1490 1500
ACRDVLMVCI PFLGLQELYG VLDRLLDQDL PRARDFYRQF CVKLAKKNQE
1510 1520 1530 1540 1550
ILWILHTHSF TFFTSTWEVI RSAAVKLTDA VVLNLTSQYV ELLDREQLTT
1560 1570 1580
RLQALRQDPC ISVQRAAEAA LQTLLRRCKE TSIPL
Length:1,585
Mass (Da):180,781
Last modified:May 18, 2010 - v3
Checksum:iA57EF4E9DC182FAD
GO
Isoform 2 (identifier: Q7Z745-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     1-296: Missing.

Note: No experimental confirmation available.
Show »
Length:1,289
Mass (Da):146,438
Checksum:iCD1E87E04BB82DDD
GO

Sequence cautioni

The sequence AAP97306 differs from that shown. Reason: Frameshift at positions 1063 and 1080.Curated
The sequence BAC05103 differs from that shown. Reason: Erroneous initiation. Translation N-terminally extended.Curated

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti624A → V in CAH18199 (PubMed:17974005).Curated1
Sequence conflicti897L → P in BAC05103 (PubMed:14702039).Curated1
Sequence conflicti1059V → L in AAP97306 (Ref. 5) Curated1
Sequence conflicti1072E → D in AAP97306 (Ref. 5) Curated1
Sequence conflicti1424I → T in CAH18199 (PubMed:17974005).Curated1
Sequence conflicti1424I → T in AAP97306 (Ref. 5) Curated1

Natural variant

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Natural variantiVAR_04298311M → V1 PublicationCorresponds to variant dbSNP:rs13174484Ensembl.1
Natural variantiVAR_042984191W → R1 PublicationCorresponds to variant dbSNP:rs865093Ensembl.1
Natural variantiVAR_042985263L → I. Corresponds to variant dbSNP:rs10045243Ensembl.1
Natural variantiVAR_042986468E → V. Corresponds to variant dbSNP:rs17198125Ensembl.1
Natural variantiVAR_042987496V → I2 PublicationsCorresponds to variant dbSNP:rs325864Ensembl.1
Natural variantiVAR_042988526R → H2 PublicationsCorresponds to variant dbSNP:rs13173930Ensembl.1
Natural variantiVAR_042989617K → N1 PublicationCorresponds to variant dbSNP:rs17854768Ensembl.1
Natural variantiVAR_042990648D → V. Corresponds to variant dbSNP:rs16870720Ensembl.1
Natural variantiVAR_042991781M → V. Corresponds to variant dbSNP:rs10067611Ensembl.1
Natural variantiVAR_042992918N → K. Corresponds to variant dbSNP:rs10054110Ensembl.1
Natural variantiVAR_0429931179L → P4 PublicationsCorresponds to variant dbSNP:rs2271704Ensembl.1

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_0333611 – 296Missing in isoform 2. 1 PublicationAdd BLAST296

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CR749346 mRNA. Translation: CAH18199.1.
AC114967 Genomic DNA. No translation available.
BC052962 mRNA. Translation: AAH52962.1.
AK097562 mRNA. Translation: BAC05103.1. Different initiation.
AF432068 mRNA. Translation: AAP97306.1. Frameshift.
CCDSiCCDS47202.1. [Q7Z745-1]
RefSeqiNP_775760.3. NM_173489.4. [Q7Z745-1]
UniGeneiHs.97714.

Genome annotation databases

EnsembliENST00000399564; ENSP00000382476; ENSG00000171495. [Q7Z745-1]
GeneIDi133558.
KEGGihsa:133558.
UCSCiuc003jmj.5. human. [Q7Z745-1]

Keywords - Coding sequence diversityi

Alternative splicing, Polymorphism

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.

Entry informationi

Entry nameiMRO2B_HUMAN
AccessioniPrimary (citable) accession number: Q7Z745
Secondary accession number(s): Q68DM1, Q7Z4U4, Q8N7X3
Entry historyiIntegrated into UniProtKB/Swiss-Prot: April 29, 2008
Last sequence update: May 18, 2010
Last modified: June 7, 2017
This is version 112 of the entry and version 3 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Human chromosome 5
    Human chromosome 5: entries, gene names and cross-references to MIM
  2. Human entries with polymorphisms or disease mutations
    List of human entries with polymorphisms or disease mutations
  3. Human polymorphisms and disease mutations
    Index of human polymorphisms and disease mutations