Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Protein

Arpin

Gene

ARPIN

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Regulates actin polymerization by inhibiting the actin-nucleating activity of the Arp2/3 complex; the function is competetive with nucleation promoting factors. Participates in an incoherent feedforward loop at the lamellipodium tip where it inhibits the ARP2/2 complex in response to Rac signaling and where Rac also stimulates actin polymerization through the WAVE complex. Involved in steering cell migration by controlling its directional persistence.1 Publication

GO - Biological processi

  • directional locomotion Source: UniProtKB
  • negative regulation of actin nucleation Source: UniProtKB
  • negative regulation of cell migration Source: UniProtKB
  • negative regulation of lamellipodium morphogenesis Source: UniProtKB
Complete GO annotation...

Names & Taxonomyi

Protein namesi
Recommended name:
Arpin
Alternative name(s):
Arp2/3 inhibition protein
Gene namesi
Name:ARPIN
Synonyms:C15orf38
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
Proteomesi
  • UP000005640 Componenti: Chromosome 15

Organism-specific databases

HGNCiHGNC:28782. ARPIN.

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cell projection

Pathology & Biotechi

Organism-specific databases

PharmGKBiPA142672274.

Polymorphism and mutation databases

BioMutaiC15orf38.
DMDMi74738824.

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 226226ArpinPRO_0000244265Add
BLAST

Proteomic databases

EPDiQ7Z6K5.
MaxQBiQ7Z6K5.
PaxDbiQ7Z6K5.
PRIDEiQ7Z6K5.
TopDownProteomicsiQ7Z6K5-1. [Q7Z6K5-1]

PTM databases

iPTMnetiQ7Z6K5.
PhosphoSiteiQ7Z6K5.

Expressioni

Gene expression databases

BgeeiQ7Z6K5.
CleanExiHS_C15orf38.
ExpressionAtlasiQ7Z6K5. baseline and differential.
GenevisibleiQ7Z6K5. HS.

Organism-specific databases

HPAiHPA040513.
HPA047173.
HPA049270.
HPA052278.

Interactioni

Subunit structurei

Associates with the Arp2/3 complex. Interacts with ARPC2; enhanced by activated RAC1. Interacts with ARPC5; the interaction is dependent on RAC1.1 Publication

Binary interactionsi

WithEntry#Exp.IntActNotes
NIF3L1Q9GZT83EBI-10258086,EBI-740897
TRIM27P143733EBI-10258086,EBI-719493

Protein-protein interaction databases

BioGridi131507. 10 interactions.
DIPiDIP-60587N.
IntActiQ7Z6K5. 4 interactions.
STRINGi9606.ENSP00000350075.

Structurei

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
4Z68X-ray1.86E211-222[»]
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni211 – 22616Necessary and sufficient for interaction with ARPC2Add
BLAST

Domaini

The acidic C-terminus is necessary and sufficient to inhibit ARP2/3 complex activity.1 Publication

Sequence similaritiesi

Belongs to the Arpin family.Curated

Phylogenomic databases

eggNOGiENOG410IGMB. Eukaryota.
ENOG4111JJD. LUCA.
GeneTreeiENSGT00530000064251.
HOGENOMiHOG000111854.
HOVERGENiHBG054275.
InParanoidiQ7Z6K5.
OMAiTHGRKER.
OrthoDBiEOG751NGM.
PhylomeDBiQ7Z6K5.
TreeFamiTF300189.

Family and domain databases

InterProiIPR018889. UPF0552.
[Graphical view]
PfamiPF10574. UPF0552. 1 hit.
[Graphical view]

Sequences (2)i

Sequence statusi: Complete.

This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 1 (identifier: Q7Z6K5-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MSRIYHDGAL RNKAVQSVRL PGAWDPAAHQ GGNGVLLEGE LIDVSRHSIL
60 70 80 90 100
DTHGRKERYY VLYIRPSHIH RRKFDAKGNE IEPNFSATRK VNTGFLMSSY
110 120 130 140 150
KVEAKGDTDR LTPEALKGLV NKPELLALTE SLTPDHTVAF WMPESEMEVM
160 170 180 190 200
ELELGAGVRL KTRGDGPFLD SLAKLEAGTV TKCNFTGDGK TGASWTDNIM
210 220
AQKCSKGAAA EIREQGDGAE DEEWDD
Length:226
Mass (Da):24,943
Last modified:October 1, 2003 - v1
Checksum:i80124CFFD6D6E455
GO
Isoform C15orf38-AP3S2 (identifier: Q7Z6K5-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     225-226: DD → PEEIQQQIVR...IKVPNLSQFV

Note: Based on a naturally occurring readthrough transcript which produces a C15orf38-AP3S2 fusion protein.
Show »
Length:394
Mass (Da):43,882
Checksum:iC6DC9DC6039C3C64
GO

Alternative sequence

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Alternative sequencei225 – 2262DD → PEEIQQQIVRETFHLVLKRD DNICNFLEGGSLIGGSDYKL IYRHYATLYFVFCVDSSESE LGILDLIQVFVETLDKCFEN VCELDLIFHMDKVHYILQEV VMGGMVLETNMNEIVAQIEA QNRLEKSEGGLSAAPARAVS AVKNINLPEIPRNINIGDLN IKVPNLSQFV in isoform C15orf38-AP3S2. 1 PublicationVSP_047416

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AK000495 mRNA. No translation available.
AC018988 Genomic DNA. No translation available.
AC027176 Genomic DNA. No translation available.
BC053602 mRNA. Translation: AAH53602.1.
CCDSiCCDS42080.1. [Q7Z6K5-1]
RefSeqiNP_001269309.1. NM_001282380.1.
NP_872422.1. NM_182616.3. [Q7Z6K5-1]
UniGeneiHs.6734.
Hs.744890.

Genome annotation databases

EnsembliENST00000357484; ENSP00000350075; ENSG00000242498. [Q7Z6K5-1]
GeneIDi348110.
KEGGihsa:348110.
UCSCiuc002bou.4. human. [Q7Z6K5-1]

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AK000495 mRNA. No translation available.
AC018988 Genomic DNA. No translation available.
AC027176 Genomic DNA. No translation available.
BC053602 mRNA. Translation: AAH53602.1.
CCDSiCCDS42080.1. [Q7Z6K5-1]
RefSeqiNP_001269309.1. NM_001282380.1.
NP_872422.1. NM_182616.3. [Q7Z6K5-1]
UniGeneiHs.6734.
Hs.744890.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
4Z68X-ray1.86E211-222[»]
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi131507. 10 interactions.
DIPiDIP-60587N.
IntActiQ7Z6K5. 4 interactions.
STRINGi9606.ENSP00000350075.

PTM databases

iPTMnetiQ7Z6K5.
PhosphoSiteiQ7Z6K5.

Polymorphism and mutation databases

BioMutaiC15orf38.
DMDMi74738824.

Proteomic databases

EPDiQ7Z6K5.
MaxQBiQ7Z6K5.
PaxDbiQ7Z6K5.
PRIDEiQ7Z6K5.
TopDownProteomicsiQ7Z6K5-1. [Q7Z6K5-1]

Protocols and materials databases

DNASUi348110.
Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENST00000357484; ENSP00000350075; ENSG00000242498. [Q7Z6K5-1]
GeneIDi348110.
KEGGihsa:348110.
UCSCiuc002bou.4. human. [Q7Z6K5-1]

Organism-specific databases

CTDi348110.
GeneCardsiARPIN.
HGNCiHGNC:28782. ARPIN.
HPAiHPA040513.
HPA047173.
HPA049270.
HPA052278.
MIMi615543. gene.
neXtProtiNX_Q7Z6K5.
PharmGKBiPA142672274.
GenAtlasiSearch...

Phylogenomic databases

eggNOGiENOG410IGMB. Eukaryota.
ENOG4111JJD. LUCA.
GeneTreeiENSGT00530000064251.
HOGENOMiHOG000111854.
HOVERGENiHBG054275.
InParanoidiQ7Z6K5.
OMAiTHGRKER.
OrthoDBiEOG751NGM.
PhylomeDBiQ7Z6K5.
TreeFamiTF300189.

Miscellaneous databases

GenomeRNAii348110.
PROiQ7Z6K5.
SOURCEiSearch...

Gene expression databases

BgeeiQ7Z6K5.
CleanExiHS_C15orf38.
ExpressionAtlasiQ7Z6K5. baseline and differential.
GenevisibleiQ7Z6K5. HS.

Family and domain databases

InterProiIPR018889. UPF0552.
[Graphical view]
PfamiPF10574. UPF0552. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "Complete sequencing and characterization of 21,243 full-length human cDNAs."
    Ota T., Suzuki Y., Nishikawa T., Otsuki T., Sugiyama T., Irie R., Wakamatsu A., Hayashi K., Sato H., Nagai K., Kimura K., Makita H., Sekine M., Obayashi M., Nishi T., Shibahara T., Tanaka T., Ishii S.
    , Yamamoto J., Saito K., Kawai Y., Isono Y., Nakamura Y., Nagahari K., Murakami K., Yasuda T., Iwayanagi T., Wagatsuma M., Shiratori A., Sudo H., Hosoiri T., Kaku Y., Kodaira H., Kondo H., Sugawara M., Takahashi M., Kanda K., Yokoi T., Furuya T., Kikkawa E., Omura Y., Abe K., Kamihara K., Katsuta N., Sato K., Tanikawa M., Yamazaki M., Ninomiya K., Ishibashi T., Yamashita H., Murakawa K., Fujimori K., Tanai H., Kimata M., Watanabe M., Hiraoka S., Chiba Y., Ishida S., Ono Y., Takiguchi S., Watanabe S., Yosida M., Hotuta T., Kusano J., Kanehori K., Takahashi-Fujii A., Hara H., Tanase T.-O., Nomura Y., Togiya S., Komai F., Hara R., Takeuchi K., Arita M., Imose N., Musashino K., Yuuki H., Oshima A., Sasaki N., Aotsuka S., Yoshikawa Y., Matsunawa H., Ichihara T., Shiohata N., Sano S., Moriya S., Momiyama H., Satoh N., Takami S., Terashima Y., Suzuki O., Nakagawa S., Senoh A., Mizoguchi H., Goto Y., Shimizu F., Wakebe H., Hishigaki H., Watanabe T., Sugiyama A., Takemoto M., Kawakami B., Yamazaki M., Watanabe K., Kumagai A., Itakura S., Fukuzumi Y., Fujimori Y., Komiyama M., Tashiro H., Tanigami A., Fujiwara T., Ono T., Yamada K., Fujii Y., Ozaki K., Hirao M., Ohmori Y., Kawabata A., Hikiji T., Kobatake N., Inagaki H., Ikema Y., Okamoto S., Okitani R., Kawakami T., Noguchi S., Itoh T., Shigeta K., Senba T., Matsumura K., Nakajima Y., Mizuno T., Morinaga M., Sasaki M., Togashi T., Oyama M., Hata H., Watanabe M., Komatsu T., Mizushima-Sugano J., Satoh T., Shirai Y., Takahashi Y., Nakagawa K., Okumura K., Nagase T., Nomura N., Kikuchi H., Masuho Y., Yamashita R., Nakai K., Yada T., Nakamura Y., Ohara O., Isogai T., Sugano S.
    Nat. Genet. 36:40-45(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM C15ORF38-AP3S2).
    Tissue: Signet-ring cell carcinoma.
  2. "Analysis of the DNA sequence and duplication history of human chromosome 15."
    Zody M.C., Garber M., Sharpe T., Young S.K., Rowen L., O'Neill K., Whittaker C.A., Kamal M., Chang J.L., Cuomo C.A., Dewar K., FitzGerald M.G., Kodira C.D., Madan A., Qin S., Yang X., Abbasi N., Abouelleil A.
    , Arachchi H.M., Baradarani L., Birditt B., Bloom S., Bloom T., Borowsky M.L., Burke J., Butler J., Cook A., DeArellano K., DeCaprio D., Dorris L. III, Dors M., Eichler E.E., Engels R., Fahey J., Fleetwood P., Friedman C., Gearin G., Hall J.L., Hensley G., Johnson E., Jones C., Kamat A., Kaur A., Locke D.P., Madan A., Munson G., Jaffe D.B., Lui A., Macdonald P., Mauceli E., Naylor J.W., Nesbitt R., Nicol R., O'Leary S.B., Ratcliffe A., Rounsley S., She X., Sneddon K.M.B., Stewart S., Sougnez C., Stone S.M., Topham K., Vincent D., Wang S., Zimmer A.R., Birren B.W., Hood L., Lander E.S., Nusbaum C.
    Nature 440:671-675(2006) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
  3. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
    Tissue: Fetal brain.
  4. Cited for: FUNCTION, DOMAIN, INTERACTION WITH ARPC2, ASSOCIATION WITH THE ARP2/3 COMPLEX.

Entry informationi

Entry nameiARPIN_HUMAN
AccessioniPrimary (citable) accession number: Q7Z6K5
Secondary accession number(s): E2QRD5
Entry historyi
Integrated into UniProtKB/Swiss-Prot: June 27, 2006
Last sequence update: October 1, 2003
Last modified: June 8, 2016
This is version 100 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. Human chromosome 15
    Human chromosome 15: entries, gene names and cross-references to MIM
  2. MIM cross-references
    Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
  3. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  4. SIMILARITY comments
    Index of protein domains and families
  5. Uncharacterized protein families (UPF)
    List of uncharacterized protein family (UPF) entries

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.