Q7Z6J8 (UB2CB_HUMAN) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Ubiquitin-conjugating enzyme E2C-binding protein
EC=6.3.2.-

Alternative name(s):
UbcH10-binding protein with a HECT-like domain

Gene names

Name:	UBE2CBP
Synonyms:	C6orf157, H10BH

Organism

Homo sapiens (Human)

Taxonomic identifier

9606 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Primates › Haplorrhini › Catarrhini › Hominidae › Homo

Protein attributes

Sequence length	389 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	E3 ubiquitin-protein ligase which accepts ubiquitin from specific E2 ubiquitin-conjugating enzymes, and transfers it to substrates, generally promoting their degradation by the proteasome. Ref.1
Pathway	Protein modification; protein ubiquitination.
Subunit structure	Interacts with UBE2C/UbcH10 (E2 ubiquitin-conjugating enzyme). In vitro, interacts with cyclin B. Ref.1
Subcellular location	Cytoplasm Potential.
Domain	The C-terminal half (AA 188-389) is able to bind cyclin B and shows a self-ubiquitinylation activity (mono-, poly, or multi-ubiquitinylation) in a HECT-like sequence dependent manner.
Post-translational modification	Ubiquitinated by UBCH10 (E2 ubiquitin-conjugating enzyme).
Sequence caution	The sequence AAH53645.1 differs from that shown. Reason: Erroneous initiation. The sequence CAI13740.2 differs from that shown. Reason: Erroneous gene model prediction. The sequence CAI14200.2 differs from that shown. Reason: Erroneous gene model prediction. The sequence CAI15282.2 differs from that shown. Reason: Erroneous gene model prediction. The sequence CAM28304.1 differs from that shown. Reason: Erroneous gene model prediction.

Ontologies

Keywords
Biological process	Ubl conjugation pathway
Cellular component	Cytoplasm
Coding sequence diversity	Polymorphism
Molecular function	Ligase
PTM	Ubl conjugation
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	ligase activity Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 389	389	Ubiquitin-conjugating enzyme E2C-binding protein		PRO_0000311190
Regions
	Region	235 – 257	23	Interaction with UBE2C
	Region	353 – 389	37	HECT-like
Natural variations
	Natural variant	174	1	T → P. Corresponds to variant rs12528542 [ dbSNP | Ensembl ].		VAR_037152
	Natural variant	379	1	V → M. Corresponds to variant rs7739323 [ dbSNP | Ensembl ].		VAR_037153
Experimental info
	Sequence conflict	223 – 226	4	ETTK → ARSC in CAB70802. Ref.6
	Sequence conflict	340	1	S → G in AAH71763. Ref.5

Sequences

	Sequence		Length	Mass (Da)
	Q7Z6J8 [UniParc]. Last modified November 13, 2007. Version 2. Checksum: 5F72BE6670863E2B	FASTA	389	43,657
	10 20 30 40 50 60 MAASAAETRV FLEVRGQLQS ALLILGEPKE GGMPMNISIM PSSLQMKTPE GCTEIQLPAE 70 80 90 100 110 120 VRLVPSSCRG LQFVVGDGLH LRLQTQAKLG TKLISMFNQS SQTQECCTFY CQSCGEVIIK 130 140 150 160 170 180 DRKLLRVLPL PSENWGALVG EWCCHPDPFA NKSLHPQEND CFIGDSFFLV NLRTSLWQQR 190 200 210 220 230 240 PELSPVEMCC VSSDNHCKLE PKANTKVICK RCKVMLGETV SSETTKFYMT EIIIQSSERS 250 260 270 280 290 300 FPIIPRSWFV QSVIAQCLVQ LSSARSTFRF TIQGQDDKVY ILLWLLNSDS LVIESLRNSK 310 320 330 340 350 360 YIKKFPLLEN TFKADSSSAW SAVKVLYQPC IKSRNEKLVS LWESDISVHP LTLPSATCLE 370 380 LLLILSKSNA NLPSSLRRVN SFQVAFLKM

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"A novel UbcH10-binding protein facilitates the ubiquitinylation of cyclin B in vitro." Kobirumaki F., Miyauchi Y., Fukami K., Tanaka H. J. Biochem. 137:133-139(2005) [PubMed: 15749827] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA], INTERACTION WITH UBE2C AND CYCLIN B, FUNCTION. Tissue: Cervix carcinoma.
[2]	"Complete sequencing and characterization of 21,243 full-length human cDNAs." Ota T., Suzuki Y., Nishikawa T., Otsuki T., Sugiyama T., Irie R., Wakamatsu A., Hayashi K., Sato H., Nagai K., Kimura K., Makita H., Sekine M., Obayashi M., Nishi T., Shibahara T., Tanaka T., Ishii S. , Yamamoto J., Saito K., Kawai Y., Isono Y., Nakamura Y., Nagahari K., Murakami K., Yasuda T., Iwayanagi T., Wagatsuma M., Shiratori A., Sudo H., Hosoiri T., Kaku Y., Kodaira H., Kondo H., Sugawara M., Takahashi M., Kanda K., Yokoi T., Furuya T., Kikkawa E., Omura Y., Abe K., Kamihara K., Katsuta N., Sato K., Tanikawa M., Yamazaki M., Ninomiya K., Ishibashi T., Yamashita H., Murakawa K., Fujimori K., Tanai H., Kimata M., Watanabe M., Hiraoka S., Chiba Y., Ishida S., Ono Y., Takiguchi S., Watanabe S., Yosida M., Hotuta T., Kusano J., Kanehori K., Takahashi-Fujii A., Hara H., Tanase T.-O., Nomura Y., Togiya S., Komai F., Hara R., Takeuchi K., Arita M., Imose N., Musashino K., Yuuki H., Oshima A., Sasaki N., Aotsuka S., Yoshikawa Y., Matsunawa H., Ichihara T., Shiohata N., Sano S., Moriya S., Momiyama H., Satoh N., Takami S., Terashima Y., Suzuki O., Nakagawa S., Senoh A., Mizoguchi H., Goto Y., Shimizu F., Wakebe H., Hishigaki H., Watanabe T., Sugiyama A., Takemoto M., Kawakami B., Yamazaki M., Watanabe K., Kumagai A., Itakura S., Fukuzumi Y., Fujimori Y., Komiyama M., Tashiro H., Tanigami A., Fujiwara T., Ono T., Yamada K., Fujii Y., Ozaki K., Hirao M., Ohmori Y., Kawabata A., Hikiji T., Kobatake N., Inagaki H., Ikema Y., Okamoto S., Okitani R., Kawakami T., Noguchi S., Itoh T., Shigeta K., Senba T., Matsumura K., Nakajima Y., Mizuno T., Morinaga M., Sasaki M., Togashi T., Oyama M., Hata H., Watanabe M., Komatsu T., Mizushima-Sugano J., Satoh T., Shirai Y., Takahashi Y., Nakagawa K., Okumura K., Nagase T., Nomura N., Kikuchi H., Masuho Y., Yamashita R., Nakai K., Yada T., Nakamura Y., Ohara O., Isogai T., Sugano S. Nat. Genet. 36:40-45(2004) [PubMed: 14702039] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
[3]	"The DNA sequence and analysis of human chromosome 6." Mungall A.J., Palmer S.A., Sims S.K., Edwards C.A., Ashurst J.L., Wilming L., Jones M.C., Horton R., Hunt S.E., Scott C.E., Gilbert J.G.R., Clamp M.E., Bethel G., Milne S., Ainscough R., Almeida J.P., Ambrose K.D., Andrews T.D. , Ashwell R.I.S., Babbage A.K., Bagguley C.L., Bailey J., Banerjee R., Barker D.J., Barlow K.F., Bates K., Beare D.M., Beasley H., Beasley O., Bird C.P., Blakey S.E., Bray-Allen S., Brook J., Brown A.J., Brown J.Y., Burford D.C., Burrill W., Burton J., Carder C., Carter N.P., Chapman J.C., Clark S.Y., Clark G., Clee C.M., Clegg S., Cobley V., Collier R.E., Collins J.E., Colman L.K., Corby N.R., Coville G.J., Culley K.M., Dhami P., Davies J., Dunn M., Earthrowl M.E., Ellington A.E., Evans K.A., Faulkner L., Francis M.D., Frankish A., Frankland J., French L., Garner P., Garnett J., Ghori M.J., Gilby L.M., Gillson C.J., Glithero R.J., Grafham D.V., Grant M., Gribble S., Griffiths C., Griffiths M.N.D., Hall R., Halls K.S., Hammond S., Harley J.L., Hart E.A., Heath P.D., Heathcott R., Holmes S.J., Howden P.J., Howe K.L., Howell G.R., Huckle E., Humphray S.J., Humphries M.D., Hunt A.R., Johnson C.M., Joy A.A., Kay M., Keenan S.J., Kimberley A.M., King A., Laird G.K., Langford C., Lawlor S., Leongamornlert D.A., Leversha M., Lloyd C.R., Lloyd D.M., Loveland J.E., Lovell J., Martin S., Mashreghi-Mohammadi M., Maslen G.L., Matthews L., McCann O.T., McLaren S.J., McLay K., McMurray A., Moore M.J.F., Mullikin J.C., Niblett D., Nickerson T., Novik K.L., Oliver K., Overton-Larty E.K., Parker A., Patel R., Pearce A.V., Peck A.I., Phillimore B.J.C.T., Phillips S., Plumb R.W., Porter K.M., Ramsey Y., Ranby S.A., Rice C.M., Ross M.T., Searle S.M., Sehra H.K., Sheridan E., Skuce C.D., Smith S., Smith M., Spraggon L., Squares S.L., Steward C.A., Sycamore N., Tamlyn-Hall G., Tester J., Theaker A.J., Thomas D.W., Thorpe A., Tracey A., Tromans A., Tubby B., Wall M., Wallis J.M., West A.P., White S.S., Whitehead S.L., Whittaker H., Wild A., Willey D.J., Wilmer T.E., Wood J.M., Wray P.W., Wyatt J.C., Young L., Younger R.M., Bentley D.R., Coulson A., Durbin R.M., Hubbard T., Sulston J.E., Dunham I., Rogers J., Beck S. Nature 425:805-811(2003) [PubMed: 14574404] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[4]	Mural R.J., Istrail S., Sutton G.G., Florea L., Halpern A.L., Mobarry C.M., Lippert R., Walenz B., Shatkay H., Dew I., Miller J.R., Flanigan M.J., Edwards N.J., Bolanos R., Fasulo D., Halldorsson B.V., Hannenhalli S., Turner R. , Yooseph S., Lu F., Nusskern D.R., Shue B.C., Zheng X.H., Zhong F., Delcher A.L., Huson D.H., Kravitz S.A., Mouchard L., Reinert K., Remington K.A., Clark A.G., Waterman M.S., Eichler E.E., Adams M.D., Hunkapiller M.W., Myers E.W., Venter J.C. Submitted (SEP-2005) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[5]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed: 15489334] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Tissue: Blood and Bone marrow.
[6]	"The full-ORF clone resource of the German cDNA consortium." Bechtel S., Rosenfelder H., Duda A., Schmidt C.P., Ernst U., Wellenreuther R., Mehrle A., Schuster C., Bahr A., Bloecker H., Heubner D., Hoerlein A., Michel G., Wedler H., Koehrer K., Ottenwaelder B., Poustka A., Wiemann S., Schupp I. BMC Genomics 8:399-399(2007) [PubMed: 17974005] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 45-226. Tissue: Testis.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AB126062 mRNA. Translation: BAD01604.1. AK298205 mRNA. Translation: BAG60475.1. AL034377 , AL139333, AL357121, AL450483 Genomic DNA. Translation: CAM28304.1. Sequence problems. AL139333 , AL034377, AL357121, AL450483 Genomic DNA. Translation: CAI15282.2. Sequence problems. AL357121 , AL034377, AL139333, AL450483 Genomic DNA. Translation: CAI13740.2. Sequence problems. AL450483 , AL034377, AL139333, AL357121 Genomic DNA. Translation: CAI14200.2. Sequence problems. CH471051 Genomic DNA. Translation: EAW48681.1. BC053645 mRNA. Translation: AAH53645.1. Different initiation. BC071763 mRNA. Translation: AAH71763.1. BC101512 mRNA. Translation: AAI01513.1. BC101538 mRNA. Translation: AAI01539.1. AL137544 mRNA. Translation: CAB70802.1.
IPI	IPI00376330.
PIR	T46387.
RefSeq	NP_944602.1. NM_198920.1.
UniGene	Hs.148609.
3D structure databases
ProteinModelPortal	Q7Z6J8.
ModBase	Search...
Protein-protein interaction databases
STRING	Q7Z6J8.
Polymorphism databases
DMDM	160395569.
Proteomic databases
PRIDE	Q7Z6J8.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
Ensembl	ENST00000237186; ENSP00000237186; ENSG00000118420.
GeneID	90025.
KEGG	hsa:90025.
UCSC	uc003pjp.1. human.
Organism-specific databases
CTD	90025.
GeneCards	GC06M083602.
H-InvDB	HIX0006033.
HGNC	HGNC:21381. UBE2CBP.
HPA	HPA027231.
MIM	612495. gene.
neXtProt	NX_Q7Z6J8.
PharmGKB	PA162407873.
GenAtlas	Search...
Phylogenomic databases
GeneTree	ENSGT00390000003986.
HOGENOM	HBG564669.
HOVERGEN	HBG056762.
InParanoid	Q7Z6J8.
OMA	FANKPLH.
PhylomeDB	Q7Z6J8.
Gene expression databases
ArrayExpress	Q7Z6J8.
Bgee	Q7Z6J8.
CleanEx	HS_UBE2CBP.
Genevestigator	Q7Z6J8.
Family and domain databases
InterPro	IPR019193. UBQ-conj_enz_E2-bd_prot. [Graphical view]
Pfam	PF09814. DUF2351. 1 hit. [Graphical view]
ProtoNet	Search...
Other
NextBio	76493.
SOURCE	Search...

Entry information

Entry name

UB2CB_HUMAN

Accession

Primary (citable) accession number: Q7Z6J8
Secondary accession number(s): B4DP63 , Q5T4W2, Q6IPR4, Q75UG0, Q9NT42

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 13, 2007
Last sequence update:	November 13, 2007
Last modified:	January 25, 2012
This is version 57 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program

Disclaimer

Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.