Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Protein

Rho GTPase-activating protein 30

Gene

ARHGAP30

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

GTPase-activating protein (GAP) for RAC1 and RHOA, but not for CDC42.1 Publication

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

GTPase activation

Enzyme and pathway databases

ReactomeiR-HSA-194840. Rho GTPase cycle.

Names & Taxonomyi

Protein namesi
Recommended name:
Rho GTPase-activating protein 30
Alternative name(s):
Rho-type GTPase-activating protein 30
Gene namesi
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
Proteomesi
  • UP000005640 Componenti: Chromosome 1

Organism-specific databases

HGNCiHGNC:27414. ARHGAP30.

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cytoplasmic vesicle

Pathology & Biotechi

Organism-specific databases

PharmGKBiPA142672585.

Polymorphism and mutation databases

BioMutaiARHGAP30.
DMDMi334302880.

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 11011101Rho GTPase-activating protein 30PRO_0000280478Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei576 – 5761PhosphoserineCombined sources
Modified residuei996 – 9961PhosphoserineCombined sources

Keywords - PTMi

Phosphoprotein

Proteomic databases

EPDiQ7Z6I6.
MaxQBiQ7Z6I6.
PaxDbiQ7Z6I6.
PeptideAtlasiQ7Z6I6.
PRIDEiQ7Z6I6.

PTM databases

iPTMnetiQ7Z6I6.
PhosphoSiteiQ7Z6I6.

Miscellaneous databases

PMAP-CutDBQ7Z6I6.

Expressioni

Gene expression databases

BgeeiQ7Z6I6.
CleanExiHS_ARHGAP30.
ExpressionAtlasiQ7Z6I6. baseline and differential.
GenevisibleiQ7Z6I6. HS.

Organism-specific databases

HPAiHPA036300.

Interactioni

Subunit structurei

Interacts with RHOU in a GTP-independent manner.1 Publication

Protein-protein interaction databases

IntActiQ7Z6I6. 3 interactions.
STRINGi9606.ENSP00000356992.

Structurei

3D structure databases

ProteinModelPortaliQ7Z6I6.
SMRiQ7Z6I6. Positions 16-217.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini20 – 215196Rho-GAPPROSITE-ProRule annotationAdd
BLAST

Compositional bias

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Compositional biasi650 – 901252Glu-richAdd
BLAST

Sequence similaritiesi

Contains 1 Rho-GAP domain.PROSITE-ProRule annotation

Phylogenomic databases

eggNOGiENOG410IPFR. Eukaryota.
ENOG410Z6GM. LUCA.
GeneTreeiENSGT00840000129752.
HOVERGENiHBG093897.
InParanoidiQ7Z6I6.
OMAiPVPNYRT.
OrthoDBiEOG7WMCHV.
PhylomeDBiQ7Z6I6.
TreeFamiTF351451.

Family and domain databases

Gene3Di1.10.555.10. 1 hit.
InterProiIPR008936. Rho_GTPase_activation_prot.
IPR000198. RhoGAP_dom.
[Graphical view]
PfamiPF00620. RhoGAP. 1 hit.
[Graphical view]
SMARTiSM00324. RhoGAP. 1 hit.
[Graphical view]
SUPFAMiSSF48350. SSF48350. 1 hit.
PROSITEiPS50238. RHOGAP. 1 hit.
[Graphical view]

Sequences (4)i

Sequence statusi: Complete.

This entry describes 4 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 1 (identifier: Q7Z6I6-1) [UniParc]FASTAAdd to basket

Also known as: L-ARHGAP30, L-30

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MKSRQKGKKK GSAKERVFGC DLQEHLQHSG QEVPQVLKSC AEFVEEYGVV
60 70 80 90 100
DGIYRLSGVS SNIQKLRQEF ESERKPDLRR DVYLQDIHCV SSLCKAYFRE
110 120 130 140 150
LPDPLLTYRL YDKFAEAVGV QLEPERLVKI LEVLRELPVP NYRTLEFLMR
160 170 180 190 200
HLVHMASFSA QTNMHARNLA IVWAPNLLRS KDIEASGFNG TAAFMEVRVQ
210 220 230 240 250
SIVVEFILTH VDQLFGGAAL SGGEVESGWR SLPGTRASGS PEDLMPRPLP
260 270 280 290 300
YHLPSILQAG DGPPQMRPYH TIIEIAEHKR KGSLKVRKWR SIFNLGRSGH
310 320 330 340 350
ETKRKLPRGA EDREDKSNKG TLRPAKSMDS LSAAAGASDE PEGLVGPSSP
360 370 380 390 400
RPSPLLPESL ENDSIEAAEG EQEPEAEALG GTNSEPGTPR AGRSAIRAGG
410 420 430 440 450
SSRAERCAGV HISDPYNVNL PLHITSILSV PPNIISNVSL ARLTRGLECP
460 470 480 490 500
ALQHRPSPAS GPGPGPGLGP GPPDEKLEAS PASSPLADSG PDDLAPALED
510 520 530 540 550
SLSQEVQDSF SFLEDSSSSE PEWVGAEDGE VAQAEAAGAA FSPGEDDPGM
560 570 580 590 600
GYLEELLGVG PQVEEFSVEP PLDDLSLDEA QFVLAPSCCS LDSAGPRPEV
610 620 630 640 650
EEENGEEVFL SAYDDLSPLL GPKPPIWKGS GSLEGEAAGC GRQALGQGGE
660 670 680 690 700
EQACWEVGED KQAEPGGRLD IREEAEGSPE TKVEAGKASE DRGEAGGSQE
710 720 730 740 750
TKVRLREGSR EETEAKEEKS KGQKKADSME AKGVEEPGGD EYTDEKEKEI
760 770 780 790 800
EREEDEQREE AQVEAGRDLE QGAQEDQVAE EKWEVVQKQE AEGVREDEDK
810 820 830 840 850
GQREKGYHEA RKDQGDGEDS RSPEAATEGG AGEVSKERES GDGEAEGDQR
860 870 880 890 900
AGGYYLEEDT LSEGSGVASL EVDCAKEGNP HSSEMEEVAP QPPQPEEMEP
910 920 930 940 950
EGQPSPDGCL CPCSLGLGGV GMRLASTLVQ VQQVRSVPVV PPKPQFAKMP
960 970 980 990 1000
SAMCSKIHVA PANPCPRPGR LDGTPGERAW GSRASRSSWR NGGSLSFDAA
1010 1020 1030 1040 1050
VALARDRQRT EAQGVRRTQT CTEGGDYCLI PRTSPCSMIS AHSPRPLSCL
1060 1070 1080 1090 1100
ELPSEGAEGS GSRSRLSLPP REPQVPDPLL SSQRRSYAFE TQANPGKGEG

L
Length:1,101
Mass (Da):118,582
Last modified:May 31, 2011 - v3
Checksum:iA2BBD3079C55C563
GO
Isoform 2 (identifier: Q7Z6I6-2) [UniParc]FASTAAdd to basket

Also known as: S-ARHGAP30, S-30

The sequence of this isoform differs from the canonical sequence as follows:
     679-889: Missing.

Note: No experimental confirmation available.
Show »
Length:890
Mass (Da):95,514
Checksum:iDE42EA5D17DE34F7
GO
Isoform 3 (identifier: Q7Z6I6-3) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     1-148: Missing.

Note: No experimental confirmation available.
Show »
Length:953
Mass (Da):101,381
Checksum:i6D6430209E6081BF
GO
Isoform 4 (identifier: Q7Z6I6-4) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     223-243: GEVESGWRSLPGTRASGSPED → QCPPCPPHIPLLGSQVSYSEP
     244-1101: Missing.

Note: No experimental confirmation available.
Show »
Length:243
Mass (Da):27,515
Checksum:i63C51237563EB79E
GO

Sequence cautioni

The sequence AAH43387.1 differs from that shown.Contaminating sequence. Potential poly-A sequence.Curated
The sequence BAD18709.1 differs from that shown. Reason: Erroneous initiation. Translation N-terminally shortened.Curated
The sequence CAI15373.1 differs from that shown. Reason: Erroneous gene model prediction. Curated
The sequence CAI15375.1 differs from that shown. Reason: Erroneous gene model prediction. Curated

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti1034 – 10341S → P in BAD18709 (Ref. 1) Curated

Natural variant

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Natural varianti70 – 701F → L.1 Publication
Corresponds to variant rs17854839 [ dbSNP | Ensembl ].
VAR_031157
Natural varianti591 – 5911L → V.2 Publications
Corresponds to variant rs3813609 [ dbSNP | Ensembl ].
VAR_031158

Alternative sequence

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Alternative sequencei1 – 148148Missing in isoform 3. 1 PublicationVSP_023732Add
BLAST
Alternative sequencei223 – 24321GEVES…GSPED → QCPPCPPHIPLLGSQVSYSE P in isoform 4. 1 PublicationVSP_023733Add
BLAST
Alternative sequencei244 – 1101858Missing in isoform 4. 1 PublicationVSP_023734Add
BLAST
Alternative sequencei679 – 889211Missing in isoform 2. 1 PublicationVSP_023735Add
BLAST

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AK160366 mRNA. Translation: BAD18709.1. Different initiation.
BX537846 mRNA. Translation: CAD97855.1.
AL591806 Genomic DNA. Translation: CAI15373.1. Sequence problems.
AL591806 Genomic DNA. Translation: CAI15374.1.
AL591806 Genomic DNA. Translation: CAI15375.1. Sequence problems.
BC043387 mRNA. Translation: AAH43387.1. Sequence problems.
BC053688 mRNA. Translation: AAH53688.1.
CCDSiCCDS1215.1. [Q7Z6I6-2]
CCDS30918.1. [Q7Z6I6-1]
RefSeqiNP_001020769.1. NM_001025598.1. [Q7Z6I6-1]
NP_001274529.1. NM_001287600.1. [Q7Z6I6-3]
NP_001274531.1. NM_001287602.1.
NP_859071.2. NM_181720.2.
XP_005245128.1. XM_005245071.3. [Q7Z6I6-3]
XP_005245130.1. XM_005245073.2. [Q7Z6I6-3]
XP_011507693.1. XM_011509391.1. [Q7Z6I6-3]
UniGeneiHs.389374.

Genome annotation databases

EnsembliENST00000368013; ENSP00000356992; ENSG00000186517. [Q7Z6I6-1]
GeneIDi257106.
KEGGihsa:257106.
UCSCiuc001fxl.4. human. [Q7Z6I6-1]

Keywords - Coding sequence diversityi

Alternative splicing, Polymorphism

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AK160366 mRNA. Translation: BAD18709.1. Different initiation.
BX537846 mRNA. Translation: CAD97855.1.
AL591806 Genomic DNA. Translation: CAI15373.1. Sequence problems.
AL591806 Genomic DNA. Translation: CAI15374.1.
AL591806 Genomic DNA. Translation: CAI15375.1. Sequence problems.
BC043387 mRNA. Translation: AAH43387.1. Sequence problems.
BC053688 mRNA. Translation: AAH53688.1.
CCDSiCCDS1215.1. [Q7Z6I6-2]
CCDS30918.1. [Q7Z6I6-1]
RefSeqiNP_001020769.1. NM_001025598.1. [Q7Z6I6-1]
NP_001274529.1. NM_001287600.1. [Q7Z6I6-3]
NP_001274531.1. NM_001287602.1.
NP_859071.2. NM_181720.2.
XP_005245128.1. XM_005245071.3. [Q7Z6I6-3]
XP_005245130.1. XM_005245073.2. [Q7Z6I6-3]
XP_011507693.1. XM_011509391.1. [Q7Z6I6-3]
UniGeneiHs.389374.

3D structure databases

ProteinModelPortaliQ7Z6I6.
SMRiQ7Z6I6. Positions 16-217.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

IntActiQ7Z6I6. 3 interactions.
STRINGi9606.ENSP00000356992.

PTM databases

iPTMnetiQ7Z6I6.
PhosphoSiteiQ7Z6I6.

Polymorphism and mutation databases

BioMutaiARHGAP30.
DMDMi334302880.

Proteomic databases

EPDiQ7Z6I6.
MaxQBiQ7Z6I6.
PaxDbiQ7Z6I6.
PeptideAtlasiQ7Z6I6.
PRIDEiQ7Z6I6.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENST00000368013; ENSP00000356992; ENSG00000186517. [Q7Z6I6-1]
GeneIDi257106.
KEGGihsa:257106.
UCSCiuc001fxl.4. human. [Q7Z6I6-1]

Organism-specific databases

CTDi257106.
GeneCardsiARHGAP30.
H-InvDBHIX0001227.
HGNCiHGNC:27414. ARHGAP30.
HPAiHPA036300.
MIMi614264. gene.
neXtProtiNX_Q7Z6I6.
PharmGKBiPA142672585.
GenAtlasiSearch...

Phylogenomic databases

eggNOGiENOG410IPFR. Eukaryota.
ENOG410Z6GM. LUCA.
GeneTreeiENSGT00840000129752.
HOVERGENiHBG093897.
InParanoidiQ7Z6I6.
OMAiPVPNYRT.
OrthoDBiEOG7WMCHV.
PhylomeDBiQ7Z6I6.
TreeFamiTF351451.

Enzyme and pathway databases

ReactomeiR-HSA-194840. Rho GTPase cycle.

Miscellaneous databases

ChiTaRSiARHGAP30. human.
GenomeRNAii257106.
PMAP-CutDBQ7Z6I6.
PROiQ7Z6I6.
SOURCEiSearch...

Gene expression databases

BgeeiQ7Z6I6.
CleanExiHS_ARHGAP30.
ExpressionAtlasiQ7Z6I6. baseline and differential.
GenevisibleiQ7Z6I6. HS.

Family and domain databases

Gene3Di1.10.555.10. 1 hit.
InterProiIPR008936. Rho_GTPase_activation_prot.
IPR000198. RhoGAP_dom.
[Graphical view]
PfamiPF00620. RhoGAP. 1 hit.
[Graphical view]
SMARTiSM00324. RhoGAP. 1 hit.
[Graphical view]
SUPFAMiSSF48350. SSF48350. 1 hit.
PROSITEiPS50238. RHOGAP. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "The nucleotide sequence of a long cDNA clone isolated from human spleen."
    Jikuya H., Takano J., Nomura N., Kikuno R., Nagase T., Ohara O.
    Submitted (APR-2004) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
    Tissue: Spleen.
  2. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 4), VARIANT VAL-591.
    Tissue: Endometrium.
  3. "The DNA sequence and biological annotation of human chromosome 1."
    Gregory S.G., Barlow K.F., McLay K.E., Kaul R., Swarbreck D., Dunham A., Scott C.E., Howe K.L., Woodfine K., Spencer C.C.A., Jones M.C., Gillson C., Searle S., Zhou Y., Kokocinski F., McDonald L., Evans R., Phillips K.
    , Atkinson A., Cooper R., Jones C., Hall R.E., Andrews T.D., Lloyd C., Ainscough R., Almeida J.P., Ambrose K.D., Anderson F., Andrew R.W., Ashwell R.I.S., Aubin K., Babbage A.K., Bagguley C.L., Bailey J., Beasley H., Bethel G., Bird C.P., Bray-Allen S., Brown J.Y., Brown A.J., Buckley D., Burton J., Bye J., Carder C., Chapman J.C., Clark S.Y., Clarke G., Clee C., Cobley V., Collier R.E., Corby N., Coville G.J., Davies J., Deadman R., Dunn M., Earthrowl M., Ellington A.G., Errington H., Frankish A., Frankland J., French L., Garner P., Garnett J., Gay L., Ghori M.R.J., Gibson R., Gilby L.M., Gillett W., Glithero R.J., Grafham D.V., Griffiths C., Griffiths-Jones S., Grocock R., Hammond S., Harrison E.S.I., Hart E., Haugen E., Heath P.D., Holmes S., Holt K., Howden P.J., Hunt A.R., Hunt S.E., Hunter G., Isherwood J., James R., Johnson C., Johnson D., Joy A., Kay M., Kershaw J.K., Kibukawa M., Kimberley A.M., King A., Knights A.J., Lad H., Laird G., Lawlor S., Leongamornlert D.A., Lloyd D.M., Loveland J., Lovell J., Lush M.J., Lyne R., Martin S., Mashreghi-Mohammadi M., Matthews L., Matthews N.S.W., McLaren S., Milne S., Mistry S., Moore M.J.F., Nickerson T., O'Dell C.N., Oliver K., Palmeiri A., Palmer S.A., Parker A., Patel D., Pearce A.V., Peck A.I., Pelan S., Phelps K., Phillimore B.J., Plumb R., Rajan J., Raymond C., Rouse G., Saenphimmachak C., Sehra H.K., Sheridan E., Shownkeen R., Sims S., Skuce C.D., Smith M., Steward C., Subramanian S., Sycamore N., Tracey A., Tromans A., Van Helmond Z., Wall M., Wallis J.M., White S., Whitehead S.L., Wilkinson J.E., Willey D.L., Williams H., Wilming L., Wray P.W., Wu Z., Coulson A., Vaudin M., Sulston J.E., Durbin R.M., Hubbard T., Wooster R., Dunham I., Carter N.P., McVean G., Ross M.T., Harrow J., Olson M.V., Beck S., Rogers J., Bentley D.R.
    Nature 441:315-321(2006) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
  4. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 2), NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 1-744 (ISOFORM 3), VARIANTS LEU-70 AND VAL-591.
    Tissue: Blood and Testis.
  5. "Robust phosphoproteomic profiling of tyrosine phosphorylation sites from human T cells using immobilized metal affinity chromatography and tandem mass spectrometry."
    Brill L.M., Salomon A.R., Ficarro S.B., Mukherji M., Stettler-Gill M., Peters E.C.
    Anal. Chem. 76:2763-2772(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    Tissue: Leukemic T-cell.
  6. "Quantitative phosphoproteomic analysis of T cell receptor signaling reveals system-wide modulation of protein-protein interactions."
    Mayya V., Lundgren D.H., Hwang S.-I., Rezaul K., Wu L., Eng J.K., Rodionov V., Han D.K.
    Sci. Signal. 2:RA46-RA46(2009) [PubMed] [Europe PMC] [Abstract]
    Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-996, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    Tissue: Leukemic T-cell.
  7. Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
  8. "ARHGAP30 is a Wrch-1-interacting protein involved in actin dynamics and cell adhesion."
    Naji L., Pacholsky D., Aspenstrom P.
    Biochem. Biophys. Res. Commun. 409:96-102(2011) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, INTERACTION WITH RHOU, SUBCELLULAR LOCATION, ALTERNATIVE SPLICING (ISOFORMS 1 AND 2).
  9. "An enzyme assisted RP-RPLC approach for in-depth analysis of human liver phosphoproteome."
    Bian Y., Song C., Cheng K., Dong M., Wang F., Huang J., Sun D., Wang L., Ye M., Zou H.
    J. Proteomics 96:253-262(2014) [PubMed] [Europe PMC] [Abstract]
    Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-576, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    Tissue: Liver.

Entry informationi

Entry nameiRHG30_HUMAN
AccessioniPrimary (citable) accession number: Q7Z6I6
Secondary accession number(s): Q5SY52
, Q5SY53, Q5SY54, Q6ZML6, Q7Z3J8, Q86XI7
Entry historyi
Integrated into UniProtKB/Swiss-Prot: March 20, 2007
Last sequence update: May 31, 2011
Last modified: July 6, 2016
This is version 105 of the entry and version 3 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Human chromosome 1
    Human chromosome 1: entries, gene names and cross-references to MIM
  2. Human entries with polymorphisms or disease mutations
    List of human entries with polymorphisms or disease mutations
  3. Human polymorphisms and disease mutations
    Index of human polymorphisms and disease mutations
  4. MIM cross-references
    Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
  5. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.