Q7Z6B7 (SRGP1_HUMAN) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
SLIT-ROBO Rho GTPase-activating protein 1
Short name=srGAP1

Alternative name(s):
Rho GTPase-activating protein 13

Gene names

Name:	SRGAP1
Synonyms:	ARHGAP13, KIAA1304

Organism

Homo sapiens (Human) [Reference proteome]

Taxonomic identifier

9606 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Deuterostomia › Chordata › Craniata › Vertebrata › Gnathostomata › Teleostomi › Euteleostomi › Sarcopterygii › Tetrapoda › Amniota › Mammalia › Theria › Eutheria › Euarchontoglires › Primates › Haplorrhini › Simiiformes › Catarrhini › Hominoidea › Hominidae › Homininae › Homo

Protein attributes

Sequence length	1085 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	GTPase-activating protein for RhoA and Cdc42 small GTPases. Together with CDC42 seems to be involved in the pathway mediating the repulsive signaling of Robo and Slit proteins in neuronal migration. SLIT2, probably through interaction with ROBO1, increases the interaction of SRGAP1 with ROBO1 and inactivates CDC42. Ref.3
Subunit structure	Homodimer Probable. Forms a heterooligomer with SRGAP2 and SRGAP3 through its F-BAR domain. Interacts with ROBO1, CDC42 and RHOA. Interacts with FASLG. Ref.3 Ref.7 Ref.10
Tissue specificity	Expressed in brain, lung, kidney, and testis. Ref.3
Domain	The F-BAR domain mediates oligomerization, binds membranes, and constrains plasma membrane protrusions. Ref.10
Sequence similarities	Contains 1 FCH domain. Contains 1 Rho-GAP domain. Contains 1 SH3 domain.
Sequence caution	The sequence BAA92542.1 differs from that shown. Reason: Erroneous initiation. The sequence BAA92542.1 differs from that shown. Reason: Intron retention.

Ontologies

Keywords
Coding sequence diversity	Alternative splicing
Domain	Coiled coil SH3 domain
Molecular function	GTPase activation
PTM	Phosphoprotein
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	Rho protein signal transduction Inferred from electronic annotation. Source: Compara axon guidance Traceable author statement. Source: Reactome cell migration Inferred from electronic annotation. Source: Compara regulation of small GTPase mediated signal transduction Traceable author statement. Source: Reactome small GTPase mediated signal transduction Traceable author statement. Source: Reactome
Cellular_component	cytosol Traceable author statement. Source: Reactome
Molecular_function	Rho GTPase activator activity Inferred from electronic annotation. Source: Compara
Complete GO annotation...

Alternative products

This entry describes 2 isoforms produced by alternative splicing. [Align] [Select]

Isoform 1 (identifier: Q7Z6B7-1)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

Isoform 2 (identifier: Q7Z6B7-2)

The sequence of this isoform differs from the canonical sequence as follows:
     479-513: RPPNVPPKPQKHRKSRPRSQYNTKLFNGDLETFVK → SRGRRNSHTRHQ

Note: No experimental confirmation available.

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 1085	1085	SLIT-ROBO Rho GTPase-activating protein 1		PRO_0000056765
Regions
	Domain	22 – 87	66	FCH
	Domain	506 – 694	189	Rho-GAP
	Domain	743 – 802	60	SH3
	Region	1 – 516	516	F-BAR domain
	Coiled coil	351 – 390	40	Potential
	Coiled coil	956 – 985	30	Potential
	Compositional bias	854 – 857	4	Poly-Pro
	Compositional bias	909 – 912	4	Poly-Arg
	Compositional bias	1027 – 1032	6	Poly-Ser
Amino acid modifications
	Modified residue	999	1	Phosphoserine Ref.6
	Modified residue	1001	1	Phosphothreonine Ref.6
	Modified residue	1032	1	Phosphoserine Ref.6
Natural variations
	Alternative sequence	479 – 513	35	RPPNV…ETFVK → SRGRRNSHTRHQ in isoform 2.		VSP_010580

Sequences

	Sequence		Length	Mass (Da)
	Isoform 1 [UniParc]. Last modified October 1, 2003. Version 1. Checksum: D7ED1F651344F0D9	FASTA	1,085	124,264
	10 20 30 40 50 60 MSTPSRFKKD KEIIAEYESQ VKEIRAQLVE QQKCLEQQTE MRVQLLQDLQ DFFRKKAEIE 70 80 90 100 110 120 TEYSRNLEKL AERFMAKTRS TKDHQQYKKD QNLLSPVNCW YLLLNQVRRE SKDHATLSDI 130 140 150 160 170 180 YLNNVIMRFM QISEDSTRMF KKSKEIAFQL HEDLMKVLNE LYTVMKTYHM YHAESISAES 190 200 210 220 230 240 KLKEAEKQEE KQIGRSGDPV FHIRLEERHQ RRSSVKKIEK MKEKRQAKYS ENKLKSIKAR 250 260 270 280 290 300 NEYLLTLEAT NASVFKYYIH DLSDLIDCCD LGYHASLNRA LRTYLSAEYN LETSRHEGLD 310 320 330 340 350 360 IIENAVDNLE PRSDKQRFME MYPAAFCPPM KFEFQSHMGD EVCQVSAQQP VQAELMLRYQ 370 380 390 400 410 420 QLQSRLATLK IENEEVKKTT EATLQTIQDM VTIEDYDVSE CFQHSRSTES VKSTVSETYL 430 440 450 460 470 480 SKPSIAKRRA NQQETEQFYF MKLREYLEGS NLITKLQAKH DLLQRTLGEG HRAEYMTTRP 490 500 510 520 530 540 PNVPPKPQKH RKSRPRSQYN TKLFNGDLET FVKDSGQVIP LIVESCIRFI NLYGLQHQGI 550 560 570 580 590 600 FRVSGSQVEV NDIKNSFERG ENPLADDQSN HDINSVAGVL KLYFRGLENP LFPKERFNDL 610 620 630 640 650 660 ISCIRIDNLY ERALHIRKLL LTLPRSVLIV MRYLFAFLNH LSQYSDENMM DPYNLAICFG 670 680 690 700 710 720 PTLMPVPEIQ DQVSCQAHVN EIIKTIIIHH ETIFPDAKEL DGPVYEKCMA GDDYCDSPYS 730 740 750 760 770 780 EHGTLEEVDQ DAGTEPHTSE DECEPIEAIA KFDYVGRSAR ELSFKKGASL LLYHRASEDW 790 800 810 820 830 840 WEGRHNGIDG LVPHQYIVVQ DMDDTFSDTL SQKADSEASS GPVTEDKSSS KDMNSPTDRH 850 860 870 880 890 900 PDGYLARQRK RGEPPPPVRR PGRTSDGHCP LHPPHALSNS SVDLGSPSLA SHPRGLLQNR 910 920 930 940 950 960 GLNNDSPERR RRPGHGSLTN ISRHDSLKKI DSPPIRRSTS SGQYTGFNDH KPLDPETIAQ 970 980 990 1000 1010 1020 DIEETMNTAL NELRELERQS TAKHAPDVVL DTLEQVKNSP TPATSTESLS PLHNVALRSS 1030 1040 1050 1060 1070 1080 EPQIRRSTSS SSDTMSTFKP MVAPRMGVQL KPPALRPKPA VLPKTNPTIG PAPPPQGPTD KSCTM
	Isoform 2 [UniParc]. Checksum: C9872F6AA2825358	FASTA	1,062	121,594
	10 20 30 40 50 60 MSTPSRFKKD KEIIAEYESQ VKEIRAQLVE QQKCLEQQTE MRVQLLQDLQ DFFRKKAEIE 70 80 90 100 110 120 TEYSRNLEKL AERFMAKTRS TKDHQQYKKD QNLLSPVNCW YLLLNQVRRE SKDHATLSDI 130 140 150 160 170 180 YLNNVIMRFM QISEDSTRMF KKSKEIAFQL HEDLMKVLNE LYTVMKTYHM YHAESISAES 190 200 210 220 230 240 KLKEAEKQEE KQIGRSGDPV FHIRLEERHQ RRSSVKKIEK MKEKRQAKYS ENKLKSIKAR 250 260 270 280 290 300 NEYLLTLEAT NASVFKYYIH DLSDLIDCCD LGYHASLNRA LRTYLSAEYN LETSRHEGLD 310 320 330 340 350 360 IIENAVDNLE PRSDKQRFME MYPAAFCPPM KFEFQSHMGD EVCQVSAQQP VQAELMLRYQ 370 380 390 400 410 420 QLQSRLATLK IENEEVKKTT EATLQTIQDM VTIEDYDVSE CFQHSRSTES VKSTVSETYL 430 440 450 460 470 480 SKPSIAKRRA NQQETEQFYF MKLREYLEGS NLITKLQAKH DLLQRTLGEG HRAEYMTTSR 490 500 510 520 530 540 GRRNSHTRHQ DSGQVIPLIV ESCIRFINLY GLQHQGIFRV SGSQVEVNDI KNSFERGENP 550 560 570 580 590 600 LADDQSNHDI NSVAGVLKLY FRGLENPLFP KERFNDLISC IRIDNLYERA LHIRKLLLTL 610 620 630 640 650 660 PRSVLIVMRY LFAFLNHLSQ YSDENMMDPY NLAICFGPTL MPVPEIQDQV SCQAHVNEII 670 680 690 700 710 720 KTIIIHHETI FPDAKELDGP VYEKCMAGDD YCDSPYSEHG TLEEVDQDAG TEPHTSEDEC 730 740 750 760 770 780 EPIEAIAKFD YVGRSARELS FKKGASLLLY HRASEDWWEG RHNGIDGLVP HQYIVVQDMD 790 800 810 820 830 840 DTFSDTLSQK ADSEASSGPV TEDKSSSKDM NSPTDRHPDG YLARQRKRGE PPPPVRRPGR 850 860 870 880 890 900 TSDGHCPLHP PHALSNSSVD LGSPSLASHP RGLLQNRGLN NDSPERRRRP GHGSLTNISR 910 920 930 940 950 960 HDSLKKIDSP PIRRSTSSGQ YTGFNDHKPL DPETIAQDIE ETMNTALNEL RELERQSTAK 970 980 990 1000 1010 1020 HAPDVVLDTL EQVKNSPTPA TSTESLSPLH NVALRSSEPQ IRRSTSSSSD TMSTFKPMVA 1030 1040 1050 1060 PRMGVQLKPP ALRPKPAVLP KTNPTIGPAP PPQGPTDKSC TM

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1). Tissue: Uterus.
[2]	"Prediction of the coding sequences of unidentified human genes. XVI. The complete sequences of 150 new cDNA clones from brain which code for large proteins in vitro." Nagase T., Kikuno R., Ishikawa K., Hirosawa M., Ohara O. DNA Res. 7:65-73(2000) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 23-1085 (ISOFORM 2). Tissue: Brain.
[3]	"Signal transduction in neuronal migration: roles of GTPase activating proteins and the small GTPase Cdc42 in the Slit-Robo pathway." Wong K., Ren X.R., Huang Y.Z., Xie Y., Liu G., Saito H., Tang H., Wen L., Brady-Kalnay S.M., Mei L., Wu J.Y., Xiong W.C., Rao Y. Cell 107:209-221(2001) [PubMed] [Europe PMC] [Abstract] Cited for: FUNCTION, TISSUE SPECIFICITY, INTERACTION WITH ROBO1; CDC42 AND RHOA.
[4]	"Global, in vivo, and site-specific phosphorylation dynamics in signaling networks." Olsen J.V., Blagoev B., Gnad F., Macek B., Kumar C., Mortensen P., Mann M. Cell 127:635-648(2006) [PubMed] [Europe PMC] [Abstract] Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS]. Tissue: Cervix carcinoma.
[5]	"Combining protein-based IMAC, peptide-based IMAC, and MudPIT for efficient phosphoproteomic analysis." Cantin G.T., Yi W., Lu B., Park S.K., Xu T., Lee J.-D., Yates J.R. III J. Proteome Res. 7:1346-1351(2008) [PubMed] [Europe PMC] [Abstract] Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS]. Tissue: Cervix carcinoma.
[6]	"A quantitative atlas of mitotic phosphorylation." Dephoure N., Zhou C., Villen J., Beausoleil S.A., Bakalarski C.E., Elledge S.J., Gygi S.P. Proc. Natl. Acad. Sci. U.S.A. 105:10762-10767(2008) [PubMed] [Europe PMC] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-999; THR-1001 AND SER-1032, MASS SPECTROMETRY. Tissue: Cervix carcinoma.
[7]	"Identification of SH3 domain interaction partners of human FasL (CD178) by phage display screening." Voss M., Lettau M., Janssen O. BMC Immunol. 10:53-53(2009) [PubMed] [Europe PMC] [Abstract] Cited for: INTERACTION WITH FASLG.
[8]	"Quantitative phosphoproteomics reveals widespread full phosphorylation site occupancy during mitosis." Olsen J.V., Vermeulen M., Santamaria A., Kumar C., Miller M.L., Jensen L.J., Gnad F., Cox J., Jensen T.S., Nigg E.A., Brunak S., Mann M. Sci. Signal. 3:RA3-RA3(2010) [PubMed] [Europe PMC] [Abstract] Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS]. Tissue: Cervix carcinoma.
[9]	"Initial characterization of the human central proteome." Burkard T.R., Planyavsky M., Kaupe I., Breitwieser F.P., Buerckstuemmer T., Bennett K.L., Superti-Furga G., Colinge J. BMC Syst. Biol. 5:17-17(2011) [PubMed] [Europe PMC] [Abstract] Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
[10]	"The F-BAR domains from srGAP1, srGAP2, and srGAP3 differentially regulate membrane deformation." Coutinho-Budd J., Ghukasyan V., Zylka M.J., Polleux F. J. Cell Sci. 125:3390-3401(2012) [PubMed] [Europe PMC] [Abstract] Cited for: HETEROOLIGOMERIZATION, F-BAR DOMAIN.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	BC053903 mRNA. Translation: AAH53903.1. AB037725 mRNA. Translation: BAA92542.1. Different initiation.
IPI	IPI00376259. IPI00414827.
PIR	G59436.
RefSeq	NP_065813.1. NM_020762.2.
UniGene	Hs.210751. Hs.593803.
3D structure databases
ProteinModelPortal	Q7Z6B7.
ModBase	Search...
Protein-protein interaction databases
IntAct	Q7Z6B7. 1 interaction.
MINT	MINT-3308471.
STRING	9606.ENSP00000347198.
PTM databases
PhosphoSite	Q7Z6B7.
Polymorphism databases
DMDM	48428624.
Proteomic databases
PaxDb	Q7Z6B7.
PRIDE	Q7Z6B7.
Protocols and materials databases
DNASU	57522.
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
Ensembl	ENST00000355086; ENSP00000347198; ENSG00000196935. ENST00000357825; ENSP00000350480; ENSG00000196935.
GeneID	57522.
KEGG	hsa:57522.
UCSC	uc001srv.2. human. uc010ssp.1. human.
Organism-specific databases
CTD	57522.
GeneCards	GC12P064238.
HGNC	HGNC:17382. SRGAP1.
HPA	HPA052416.
MIM	606523. gene.
neXtProt	NX_Q7Z6B7.
PharmGKB	PA134887956.
HUGE	Search...
GenAtlas	Search...
Phylogenomic databases
eggNOG	NOG264793.
HOGENOM	HOG000039980.
HOVERGEN	HBG051637.
InParanoid	Q7Z6B7.
KO	K07526.
OMA	ADKSCTM.
OrthoDB	EOG4SJ5D1.
PhylomeDB	Q7Z6B7.
Enzyme and pathway databases
Reactome	REACT_111045. Developmental Biology. REACT_111102. Signal Transduction. REACT_96538. Developmental Biology.
Gene expression databases
ArrayExpress	Q7Z6B7.
Bgee	Q7Z6B7.
CleanEx	HS_SRGAP1.
Genevestigator	Q7Z6B7.
GermOnline	ENSG00000196935. Homo sapiens.
Family and domain databases
Gene3D	1.10.555.10. 1 hit.
InterPro	IPR001060. FCH_dom. IPR008936. Rho_GTPase_activation_prot. IPR000198. RhoGAP_dom. IPR001452. SH3_domain. [Graphical view]
Pfam	PF00611. FCH. 1 hit. PF00620. RhoGAP. 1 hit. PF00018. SH3_1. 1 hit. [Graphical view]
SMART	SM00055. FCH. 1 hit. SM00324. RhoGAP. 1 hit. SM00326. SH3. 1 hit. [Graphical view]
SUPFAM	SSF48350. Rho_GAP. 1 hit. SSF50044. SH3. 1 hit.
PROSITE	PS50133. FCH. 1 hit. PS50238. RHOGAP. 1 hit. PS50002. SH3. 1 hit. [Graphical view]
ProtoNet	Search...
Other
ChiTaRS	SRGAP1. human.
GenomeRNAi	57522.
NextBio	63902.
SOURCE	Search...

Entry information

Entry name

SRGP1_HUMAN

Accession

Primary (citable) accession number: Q7Z6B7
Secondary accession number(s): Q9H8A3, Q9P2P2

Entry history

Integrated into UniProtKB/Swiss-Prot:	June 7, 2004
Last sequence update:	October 1, 2003
Last modified:	May 1, 2013
This is version 97 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program

Disclaimer

Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.