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Q7Z699

- SPRE1_HUMAN

UniProt

Q7Z699 - SPRE1_HUMAN

Protein

Sprouty-related, EVH1 domain-containing protein 1

Gene

SPRED1

Organism
Homo sapiens (Human)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 103 (01 Oct 2014)
      Sequence version 2 (04 Jan 2005)
      Previous versions | rss
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    Functioni

    Tyrosine kinase substrate that inhibits growth-factor-mediated activation of MAP kinase. Negatively regulates hematopoiesis of bone marrow By similarity.By similarity

    GO - Molecular functioni

    1. phosphatase binding Source: UniProtKB
    2. protein binding Source: IntAct
    3. protein kinase binding Source: BHF-UCL
    4. protein serine/threonine kinase inhibitor activity Source: BHF-UCL
    5. stem cell factor receptor binding Source: UniProtKB

    GO - Biological processi

    1. inactivation of MAPK activity Source: UniProtKB
    2. multicellular organismal development Source: InterPro
    3. negative regulation of peptidyl-threonine phosphorylation Source: BHF-UCL
    4. negative regulation of phosphatase activity Source: UniProtKB
    5. positive regulation of DNA damage response, signal transduction by p53 class mediator Source: BHF-UCL
    6. regulation of protein deacetylation Source: BHF-UCL

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Sprouty-related, EVH1 domain-containing protein 1
    Short name:
    Spred-1
    Short name:
    hSpred1
    Gene namesi
    Name:SPRED1
    OrganismiHomo sapiens (Human)
    Taxonomic identifieri9606 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
    ProteomesiUP000005640: Chromosome 15

    Organism-specific databases

    HGNCiHGNC:20249. SPRED1.

    Subcellular locationi

    Cell membrane 1 Publication; Peripheral membrane protein 1 Publication. Membranecaveola 1 Publication; Peripheral membrane protein 1 Publication. Nucleus 1 Publication
    Note: Localized in cholesterol-rich membrane raft/caveola fractions.

    GO - Cellular componenti

    1. caveola Source: UniProtKB-SubCell
    2. cytoplasm Source: HPA
    3. nucleus Source: HPA

    Keywords - Cellular componenti

    Cell membrane, Membrane, Nucleus

    Pathology & Biotechi

    Involvement in diseasei

    Neurofibromatosis 1-like syndrome (NFLS) [MIM:611431]: A disorder characterized mainly by cafe au lait macules without neurofibromas or other tumor manifestations of neurofibromatosis type 1, axillary freckling, and macrocephaly. Additional clinical manifestations include Noonan-like facial dysmorphism, lipomas, learning disabilities and attention deficit-hyperactivity.4 Publications
    Note: The disease is caused by mutations affecting the gene represented in this entry.
    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Natural varianti31 – 311W → C in NFLS. 1 Publication
    VAR_064827
    Natural varianti44 – 441V → D in NFLS. 2 Publications
    VAR_064828

    Keywords - Diseasei

    Disease mutation

    Organism-specific databases

    MIMi611431. phenotype.
    Orphaneti137605. Legius syndrome.
    PharmGKBiPA134897382.

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Initiator methioninei1 – 11Removed1 Publication
    Chaini2 – 444443Sprouty-related, EVH1 domain-containing protein 1PRO_0000076907Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Modified residuei2 – 21N-acetylserine1 Publication
    Modified residuei238 – 2381PhosphoserineBy similarity

    Post-translational modificationi

    Phosphorylated on tyrosine.By similarity

    Keywords - PTMi

    Acetylation, Phosphoprotein

    Proteomic databases

    MaxQBiQ7Z699.
    PaxDbiQ7Z699.
    PRIDEiQ7Z699.

    PTM databases

    PhosphoSiteiQ7Z699.

    Expressioni

    Tissue specificityi

    Weakly expressed in embryonic cell line HEK293.1 Publication

    Gene expression databases

    ArrayExpressiQ7Z699.
    BgeeiQ7Z699.
    CleanExiHS_SPRED1.
    GenevestigatoriQ7Z699.

    Organism-specific databases

    HPAiHPA042193.

    Interactioni

    Subunit structurei

    Interacts with Ras. Interacts with TAOK2 and TESK1 By similarity. Homodimer and heterodimer. Interacts with CAV1. Able to interact with SPRED2 to form heterodimers.By similarity2 Publications

    Binary interactionsi

    WithEntry#Exp.IntActNotes
    PPP1CAP621364EBI-5235340,EBI-357253
    ZDHHC17Q8IUH53EBI-5235340,EBI-524753

    Protein-protein interaction databases

    BioGridi127800. 14 interactions.
    IntActiQ7Z699. 12 interactions.
    MINTiMINT-1198540.
    STRINGi9606.ENSP00000299084.

    Structurei

    Secondary structure

    1
    444
    Legend: HelixTurnBeta strand
    Show more details
    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Beta strandi13 – 2412
    Beta strandi26 – 294
    Beta strandi31 – 377
    Beta strandi40 – 5112
    Beta strandi54 – 6411
    Turni65 – 673
    Beta strandi70 – 756
    Beta strandi81 – 866
    Beta strandi89 – 946
    Beta strandi97 – 1059
    Helixi106 – 12116
    Helixi122 – 1243

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    EntryMethodResolution (Å)ChainPositionsPDBsum
    3SYXX-ray2.45A13-131[»]
    ProteinModelPortaliQ7Z699.
    SMRiQ7Z699. Positions 13-127.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Domaini6 – 123118WH1PROSITE-ProRule annotationAdd
    BLAST
    Domaini233 – 28553KBDPROSITE-ProRule annotationAdd
    BLAST
    Domaini334 – 442109SPRPROSITE-ProRule annotationAdd
    BLAST

    Compositional bias

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Compositional biasi147 – 1504Poly-Ser

    Sequence similaritiesi

    Contains 1 KBD domain.PROSITE-ProRule annotation
    Contains 1 SPR (sprouty) domain.PROSITE-ProRule annotation
    Contains 1 WH1 domain.PROSITE-ProRule annotation

    Phylogenomic databases

    eggNOGiNOG277322.
    HOGENOMiHOG000220886.
    HOVERGENiHBG057556.
    InParanoidiQ7Z699.
    KOiK04703.
    OMAiCVTVFKV.
    OrthoDBiEOG7T7GV7.
    PhylomeDBiQ7Z699.
    TreeFamiTF321411.

    Family and domain databases

    Gene3Di2.30.29.30. 1 hit.
    InterProiIPR023337. KBD.
    IPR011993. PH_like_dom.
    IPR007875. Sprouty.
    IPR000697. WH1/EVH1.
    [Graphical view]
    PfamiPF05210. Sprouty. 1 hit.
    PF00568. WH1. 1 hit.
    [Graphical view]
    PROSITEiPS51488. KBD. 1 hit.
    PS51227. SPR. 1 hit.
    PS50229. WH1. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Sequence processingi: The displayed sequence is further processed into a mature form.

    Q7Z699-1 [UniParc]FASTAAdd to Basket

    « Hide

    MSEETATSDN DNSYARVRAV VMTRDDSSGG WLPLGGSGLS SVTVFKVPHQ    50
    EENGCADFFI RGERLRDKMV VLECMLKKDL IYNKVTPTFH HWKIDDKKFG 100
    LTFQSPADAR AFDRGIRRAI EDISQGCPES KNEAEGADDL QANEEDSSSS 150
    LVKDHLFQQE TVVTSEPYRS SNIRPSPFED LNARRVYMQS QANQITFGQP 200
    GLDIQSRSME YVQRQISKEC GSLKSQNRVP LKSIRHVSFQ DEDEIVRINP 250
    RDILIRRYAD YRHPDMWKND LERDDADSSI QFSKPDSKKS DYLYSCGDET 300
    KLSSPKDSVV FKTQPSSLKI KKSKRRKEDG ERSRCVYCQE RFNHEENVRG 350
    KCQDAPDPIK RCIYQVSCML CAESMLYHCM SDSEGDFSDP CSCDTSDDKF 400
    CLRWLALVAL SFIVPCMCCY VPLRMCHRCG EACGCCGGKH KAAG 444
    Length:444
    Mass (Da):50,477
    Last modified:January 4, 2005 - v2
    Checksum:i6FFDECCE590DB311
    GO

    Sequence cautioni

    The sequence AAH18015.1 differs from that shown. Reason: Contaminating sequence. Potential poly-A sequence.

    Experimental Info

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sequence conflicti6 – 72AT → GL in AAP59414. (PubMed:15683364)Curated

    Natural variant

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Natural varianti31 – 311W → C in NFLS. 1 Publication
    VAR_064827
    Natural varianti44 – 441V → D in NFLS. 2 Publications
    VAR_064828

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AY299089 mRNA. Translation: AAP59414.1.
    AK091222 mRNA. Translation: BAC03614.1.
    CH471125 Genomic DNA. Translation: EAW92368.1.
    BC018015 mRNA. Translation: AAH18015.1. Sequence problems.
    BC137480 mRNA. Translation: AAI37481.1.
    BC137481 mRNA. Translation: AAI37482.1.
    CCDSiCCDS32193.1.
    RefSeqiNP_689807.1. NM_152594.2.
    UniGeneiHs.525781.

    Genome annotation databases

    EnsembliENST00000299084; ENSP00000299084; ENSG00000166068.
    GeneIDi161742.
    KEGGihsa:161742.
    UCSCiuc001zka.4. human.

    Polymorphism databases

    DMDMi57013078.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AY299089 mRNA. Translation: AAP59414.1 .
    AK091222 mRNA. Translation: BAC03614.1 .
    CH471125 Genomic DNA. Translation: EAW92368.1 .
    BC018015 mRNA. Translation: AAH18015.1 . Sequence problems.
    BC137480 mRNA. Translation: AAI37481.1 .
    BC137481 mRNA. Translation: AAI37482.1 .
    CCDSi CCDS32193.1.
    RefSeqi NP_689807.1. NM_152594.2.
    UniGenei Hs.525781.

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    Entry Method Resolution (Å) Chain Positions PDBsum
    3SYX X-ray 2.45 A 13-131 [» ]
    ProteinModelPortali Q7Z699.
    SMRi Q7Z699. Positions 13-127.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    BioGridi 127800. 14 interactions.
    IntActi Q7Z699. 12 interactions.
    MINTi MINT-1198540.
    STRINGi 9606.ENSP00000299084.

    PTM databases

    PhosphoSitei Q7Z699.

    Polymorphism databases

    DMDMi 57013078.

    Proteomic databases

    MaxQBi Q7Z699.
    PaxDbi Q7Z699.
    PRIDEi Q7Z699.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    Ensembli ENST00000299084 ; ENSP00000299084 ; ENSG00000166068 .
    GeneIDi 161742.
    KEGGi hsa:161742.
    UCSCi uc001zka.4. human.

    Organism-specific databases

    CTDi 161742.
    GeneCardsi GC15P038545.
    GeneReviewsi SPRED1.
    HGNCi HGNC:20249. SPRED1.
    HPAi HPA042193.
    MIMi 609291. gene.
    611431. phenotype.
    neXtProti NX_Q7Z699.
    Orphaneti 137605. Legius syndrome.
    PharmGKBi PA134897382.
    GenAtlasi Search...

    Phylogenomic databases

    eggNOGi NOG277322.
    HOGENOMi HOG000220886.
    HOVERGENi HBG057556.
    InParanoidi Q7Z699.
    KOi K04703.
    OMAi CVTVFKV.
    OrthoDBi EOG7T7GV7.
    PhylomeDBi Q7Z699.
    TreeFami TF321411.

    Miscellaneous databases

    ChiTaRSi SPRED1. human.
    GeneWikii SPRED1.
    GenomeRNAii 161742.
    NextBioi 88102.
    PROi Q7Z699.
    SOURCEi Search...

    Gene expression databases

    ArrayExpressi Q7Z699.
    Bgeei Q7Z699.
    CleanExi HS_SPRED1.
    Genevestigatori Q7Z699.

    Family and domain databases

    Gene3Di 2.30.29.30. 1 hit.
    InterProi IPR023337. KBD.
    IPR011993. PH_like_dom.
    IPR007875. Sprouty.
    IPR000697. WH1/EVH1.
    [Graphical view ]
    Pfami PF05210. Sprouty. 1 hit.
    PF00568. WH1. 1 hit.
    [Graphical view ]
    PROSITEi PS51488. KBD. 1 hit.
    PS51227. SPR. 1 hit.
    PS50229. WH1. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. Cited for: NUCLEOTIDE SEQUENCE [MRNA], INTERACTION WITH SPRED2.
      Tissue: Glioblastoma.
    2. "Complete sequencing and characterization of 21,243 full-length human cDNAs."
      Ota T., Suzuki Y., Nishikawa T., Otsuki T., Sugiyama T., Irie R., Wakamatsu A., Hayashi K., Sato H., Nagai K., Kimura K., Makita H., Sekine M., Obayashi M., Nishi T., Shibahara T., Tanaka T., Ishii S.
      , Yamamoto J., Saito K., Kawai Y., Isono Y., Nakamura Y., Nagahari K., Murakami K., Yasuda T., Iwayanagi T., Wagatsuma M., Shiratori A., Sudo H., Hosoiri T., Kaku Y., Kodaira H., Kondo H., Sugawara M., Takahashi M., Kanda K., Yokoi T., Furuya T., Kikkawa E., Omura Y., Abe K., Kamihara K., Katsuta N., Sato K., Tanikawa M., Yamazaki M., Ninomiya K., Ishibashi T., Yamashita H., Murakawa K., Fujimori K., Tanai H., Kimata M., Watanabe M., Hiraoka S., Chiba Y., Ishida S., Ono Y., Takiguchi S., Watanabe S., Yosida M., Hotuta T., Kusano J., Kanehori K., Takahashi-Fujii A., Hara H., Tanase T.-O., Nomura Y., Togiya S., Komai F., Hara R., Takeuchi K., Arita M., Imose N., Musashino K., Yuuki H., Oshima A., Sasaki N., Aotsuka S., Yoshikawa Y., Matsunawa H., Ichihara T., Shiohata N., Sano S., Moriya S., Momiyama H., Satoh N., Takami S., Terashima Y., Suzuki O., Nakagawa S., Senoh A., Mizoguchi H., Goto Y., Shimizu F., Wakebe H., Hishigaki H., Watanabe T., Sugiyama A., Takemoto M., Kawakami B., Yamazaki M., Watanabe K., Kumagai A., Itakura S., Fukuzumi Y., Fujimori Y., Komiyama M., Tashiro H., Tanigami A., Fujiwara T., Ono T., Yamada K., Fujii Y., Ozaki K., Hirao M., Ohmori Y., Kawabata A., Hikiji T., Kobatake N., Inagaki H., Ikema Y., Okamoto S., Okitani R., Kawakami T., Noguchi S., Itoh T., Shigeta K., Senba T., Matsumura K., Nakajima Y., Mizuno T., Morinaga M., Sasaki M., Togashi T., Oyama M., Hata H., Watanabe M., Komatsu T., Mizushima-Sugano J., Satoh T., Shirai Y., Takahashi Y., Nakagawa K., Okumura K., Nagase T., Nomura N., Kikuchi H., Masuho Y., Yamashita R., Nakai K., Yada T., Nakamura Y., Ohara O., Isogai T., Sugano S.
      Nat. Genet. 36:40-45(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
      Tissue: Tongue.
    3. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    4. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
      The MGC Project Team
      Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
      Tissue: Brain.
    5. "Expression and subcellular localization of Spred proteins in mouse and human tissues."
      Engelhardt C.M., Bundschu K., Messerschmitt M., Renne T., Walter U., Reinhard M., Schuh K.
      Histochem. Cell Biol. 122:527-538(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: TISSUE SPECIFICITY.
    6. "The Sprouty-related protein, Spred-1, localizes in a lipid raft/caveola and inhibits ERK activation in collaboration with caveolin-1."
      Nonami A., Taketomi T., Kimura A., Saeki K., Takaki H., Sanada T., Taniguchi K., Harada M., Kato R., Yoshimura A.
      Genes Cells 10:887-895(2005) [PubMed] [Europe PMC] [Abstract]
      Cited for: INTERACTION WITH CAV1, SUBCELLULAR LOCATION.
    7. Cited for: INVOLVEMENT IN NFLS.
    8. Cited for: ACETYLATION [LARGE SCALE ANALYSIS] AT SER-2, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS], CLEAVAGE OF INITIATOR METHIONINE [LARGE SCALE ANALYSIS].
    9. "SPRED1 mutations (Legius syndrome): another clinically useful genotype for dissecting the neurofibromatosis type 1 phenotype."
      Spurlock G., Bennett E., Chuzhanova N., Thomas N., Jim H.P., Side L., Davies S., Haan E., Kerr B., Huson S.M., Upadhyaya M.
      J. Med. Genet. 46:431-437(2009) [PubMed] [Europe PMC] [Abstract]
      Cited for: VARIANT NFLS ASP-44.
    10. "Novel human pathological mutations. Gene symbol: SPRED1. Disease: Legius syndrome."
      Jim H.P., Upadhyaya M.
      Hum. Genet. 127:112-112(2010) [PubMed] [Europe PMC] [Abstract]
      Cited for: VARIANT NFLS ASP-44.
    11. Cited for: VARIANT NFLS CYS-31.

    Entry informationi

    Entry nameiSPRE1_HUMAN
    AccessioniPrimary (citable) accession number: Q7Z699
    Secondary accession number(s): B2RPJ8, Q05D53, Q8N256
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: January 4, 2005
    Last sequence update: January 4, 2005
    Last modified: October 1, 2014
    This is version 103 of the entry and version 2 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program
    DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

    Miscellaneousi

    Keywords - Technical termi

    3D-structure, Complete proteome, Reference proteome

    Documents

    1. Human chromosome 15
      Human chromosome 15: entries, gene names and cross-references to MIM
    2. Human entries with polymorphisms or disease mutations
      List of human entries with polymorphisms or disease mutations
    3. Human polymorphisms and disease mutations
      Index of human polymorphisms and disease mutations
    4. MIM cross-references
      Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
    5. PDB cross-references
      Index of Protein Data Bank (PDB) cross-references
    6. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3