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Protein

Sprouty-related, EVH1 domain-containing protein 1

Gene

SPRED1

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Tyrosine kinase substrate that inhibits growth-factor-mediated activation of MAP kinase. Negatively regulates hematopoiesis of bone marrow (By similarity).By similarity

GO - Molecular functioni

  • phosphatase binding Source: UniProtKB
  • protein kinase binding Source: BHF-UCL
  • protein serine/threonine kinase inhibitor activity Source: BHF-UCL
  • stem cell factor receptor binding Source: UniProtKB

GO - Biological processi

  • fibroblast growth factor receptor signaling pathway Source: Reactome
  • inactivation of MAPK activity Source: UniProtKB
  • MAPK cascade Source: Reactome
  • multicellular organism development Source: InterPro
  • negative regulation of peptidyl-threonine phosphorylation Source: BHF-UCL
  • negative regulation of phosphatase activity Source: UniProtKB
  • positive regulation of DNA damage response, signal transduction by p53 class mediator Source: BHF-UCL
  • regulation of protein deacetylation Source: BHF-UCL
Complete GO annotation...

Enzyme and pathway databases

ReactomeiR-HSA-5658442. Regulation of RAS by GAPs.
R-HSA-5658623. FGFRL1 modulation of FGFR1 signaling.
R-HSA-8849471. PTK6 Regulates RHO GTPases, RAS GTPase and MAP kinases.

Names & Taxonomyi

Protein namesi
Recommended name:
Sprouty-related, EVH1 domain-containing protein 1
Short name:
Spred-1
Short name:
hSpred1
Gene namesi
Name:SPRED1
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
Proteomesi
  • UP000005640 Componenti: Chromosome 15

Organism-specific databases

HGNCiHGNC:20249. SPRED1.

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cell membrane, Membrane, Nucleus

Pathology & Biotechi

Involvement in diseasei

Neurofibromatosis 1-like syndrome (NFLS)4 Publications
The disease is caused by mutations affecting the gene represented in this entry.
Disease descriptionA disorder characterized mainly by cafe au lait macules without neurofibromas or other tumor manifestations of neurofibromatosis type 1, axillary freckling, and macrocephaly. Additional clinical manifestations include Noonan-like facial dysmorphism, lipomas, learning disabilities and attention deficit-hyperactivity.
See also OMIM:611431
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Natural variantiVAR_06482731W → C in NFLS. 1 Publication1
Natural variantiVAR_06482844V → D in NFLS. 2 PublicationsCorresponds to variant rs121434318dbSNPEnsembl.1

Keywords - Diseasei

Disease mutation

Organism-specific databases

DisGeNETi161742.
MalaCardsiSPRED1.
MIMi611431. phenotype.
OpenTargetsiENSG00000166068.
Orphaneti137605. Legius syndrome.
PharmGKBiPA134897382.

Polymorphism and mutation databases

BioMutaiSPRED1.
DMDMi57013078.

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Initiator methionineiRemovedCombined sources
ChainiPRO_00000769072 – 444Sprouty-related, EVH1 domain-containing protein 1Add BLAST443

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei2N-acetylserineCombined sources1
Modified residuei224N6-methyllysineCombined sources1
Modified residuei238PhosphoserineCombined sources1
Modified residuei308PhosphoserineCombined sources1

Post-translational modificationi

Phosphorylated on tyrosine.By similarity

Keywords - PTMi

Acetylation, Methylation, Phosphoprotein

Proteomic databases

EPDiQ7Z699.
MaxQBiQ7Z699.
PaxDbiQ7Z699.
PeptideAtlasiQ7Z699.
PRIDEiQ7Z699.

PTM databases

iPTMnetiQ7Z699.
PhosphoSitePlusiQ7Z699.
SwissPalmiQ7Z699.

Expressioni

Tissue specificityi

Weakly expressed in embryonic cell line HEK293.1 Publication

Gene expression databases

BgeeiENSG00000166068.
CleanExiHS_SPRED1.
ExpressionAtlasiQ7Z699. baseline and differential.
GenevisibleiQ7Z699. HS.

Organism-specific databases

HPAiHPA042193.

Interactioni

Subunit structurei

Interacts with Ras. Interacts with TAOK2 and TESK1 (By similarity). Homodimer and heterodimer. Interacts with CAV1. Able to interact with SPRED2 to form heterodimers.By similarity2 Publications

Binary interactionsi

WithEntry#Exp.IntActNotes
PPP1CAP621364EBI-5235340,EBI-357253
ZDHHC17Q8IUH53EBI-5235340,EBI-524753

GO - Molecular functioni

  • phosphatase binding Source: UniProtKB
  • protein kinase binding Source: BHF-UCL
  • stem cell factor receptor binding Source: UniProtKB

Protein-protein interaction databases

BioGridi127800. 15 interactors.
IntActiQ7Z699. 14 interactors.
MINTiMINT-1198540.
STRINGi9606.ENSP00000299084.

Structurei

Secondary structure

1444
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Beta strandi13 – 24Combined sources12
Beta strandi26 – 29Combined sources4
Beta strandi31 – 37Combined sources7
Beta strandi40 – 51Combined sources12
Beta strandi54 – 64Combined sources11
Turni65 – 67Combined sources3
Beta strandi70 – 75Combined sources6
Beta strandi81 – 86Combined sources6
Beta strandi89 – 94Combined sources6
Beta strandi97 – 105Combined sources9
Helixi106 – 121Combined sources16
Helixi122 – 124Combined sources3

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
3SYXX-ray2.45A13-131[»]
ProteinModelPortaliQ7Z699.
SMRiQ7Z699.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini6 – 123WH1PROSITE-ProRule annotationAdd BLAST118
Domaini233 – 285KBDPROSITE-ProRule annotationAdd BLAST53
Domaini334 – 442SPRPROSITE-ProRule annotationAdd BLAST109

Compositional bias

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Compositional biasi147 – 150Poly-Ser4

Sequence similaritiesi

Contains 1 KBD domain.PROSITE-ProRule annotation
Contains 1 SPR (sprouty) domain.PROSITE-ProRule annotation
Contains 1 WH1 domain.PROSITE-ProRule annotation

Phylogenomic databases

eggNOGiENOG410IQ20. Eukaryota.
ENOG4112CZ7. LUCA.
GeneTreeiENSGT00730000110272.
HOGENOMiHOG000220886.
HOVERGENiHBG057556.
InParanoidiQ7Z699.
KOiK04703.
OMAiISQGCPA.
OrthoDBiEOG091G0OE8.
PhylomeDBiQ7Z699.
TreeFamiTF321411.

Family and domain databases

Gene3Di2.30.29.30. 1 hit.
InterProiIPR023337. KBD.
IPR011993. PH_dom-like.
IPR007875. Sprouty.
IPR000697. WH1/EVH1_dom.
[Graphical view]
PfamiPF05210. Sprouty. 1 hit.
PF00568. WH1. 1 hit.
[Graphical view]
SMARTiSM00461. WH1. 1 hit.
[Graphical view]
SUPFAMiSSF50729. SSF50729. 1 hit.
PROSITEiPS51488. KBD. 1 hit.
PS51227. SPR. 1 hit.
PS50229. WH1. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q7Z699-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSEETATSDN DNSYARVRAV VMTRDDSSGG WLPLGGSGLS SVTVFKVPHQ
60 70 80 90 100
EENGCADFFI RGERLRDKMV VLECMLKKDL IYNKVTPTFH HWKIDDKKFG
110 120 130 140 150
LTFQSPADAR AFDRGIRRAI EDISQGCPES KNEAEGADDL QANEEDSSSS
160 170 180 190 200
LVKDHLFQQE TVVTSEPYRS SNIRPSPFED LNARRVYMQS QANQITFGQP
210 220 230 240 250
GLDIQSRSME YVQRQISKEC GSLKSQNRVP LKSIRHVSFQ DEDEIVRINP
260 270 280 290 300
RDILIRRYAD YRHPDMWKND LERDDADSSI QFSKPDSKKS DYLYSCGDET
310 320 330 340 350
KLSSPKDSVV FKTQPSSLKI KKSKRRKEDG ERSRCVYCQE RFNHEENVRG
360 370 380 390 400
KCQDAPDPIK RCIYQVSCML CAESMLYHCM SDSEGDFSDP CSCDTSDDKF
410 420 430 440
CLRWLALVAL SFIVPCMCCY VPLRMCHRCG EACGCCGGKH KAAG
Length:444
Mass (Da):50,477
Last modified:January 4, 2005 - v2
Checksum:i6FFDECCE590DB311
GO

Sequence cautioni

The sequence AAH18015 differs from that shown. Contaminating sequence. Potential poly-A sequence.Curated

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti6 – 7AT → GL in AAP59414 (PubMed:15683364).Curated2

Natural variant

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Natural variantiVAR_06482731W → C in NFLS. 1 Publication1
Natural variantiVAR_06482844V → D in NFLS. 2 PublicationsCorresponds to variant rs121434318dbSNPEnsembl.1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AY299089 mRNA. Translation: AAP59414.1.
AK091222 mRNA. Translation: BAC03614.1.
CH471125 Genomic DNA. Translation: EAW92368.1.
BC018015 mRNA. Translation: AAH18015.1. Sequence problems.
BC137480 mRNA. Translation: AAI37481.1.
BC137481 mRNA. Translation: AAI37482.1.
CCDSiCCDS32193.1.
RefSeqiNP_689807.1. NM_152594.2.
UniGeneiHs.525781.

Genome annotation databases

EnsembliENST00000299084; ENSP00000299084; ENSG00000166068.
GeneIDi161742.
KEGGihsa:161742.
UCSCiuc001zka.5. human.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AY299089 mRNA. Translation: AAP59414.1.
AK091222 mRNA. Translation: BAC03614.1.
CH471125 Genomic DNA. Translation: EAW92368.1.
BC018015 mRNA. Translation: AAH18015.1. Sequence problems.
BC137480 mRNA. Translation: AAI37481.1.
BC137481 mRNA. Translation: AAI37482.1.
CCDSiCCDS32193.1.
RefSeqiNP_689807.1. NM_152594.2.
UniGeneiHs.525781.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
3SYXX-ray2.45A13-131[»]
ProteinModelPortaliQ7Z699.
SMRiQ7Z699.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi127800. 15 interactors.
IntActiQ7Z699. 14 interactors.
MINTiMINT-1198540.
STRINGi9606.ENSP00000299084.

PTM databases

iPTMnetiQ7Z699.
PhosphoSitePlusiQ7Z699.
SwissPalmiQ7Z699.

Polymorphism and mutation databases

BioMutaiSPRED1.
DMDMi57013078.

Proteomic databases

EPDiQ7Z699.
MaxQBiQ7Z699.
PaxDbiQ7Z699.
PeptideAtlasiQ7Z699.
PRIDEiQ7Z699.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENST00000299084; ENSP00000299084; ENSG00000166068.
GeneIDi161742.
KEGGihsa:161742.
UCSCiuc001zka.5. human.

Organism-specific databases

CTDi161742.
DisGeNETi161742.
GeneCardsiSPRED1.
GeneReviewsiSPRED1.
HGNCiHGNC:20249. SPRED1.
HPAiHPA042193.
MalaCardsiSPRED1.
MIMi609291. gene.
611431. phenotype.
neXtProtiNX_Q7Z699.
OpenTargetsiENSG00000166068.
Orphaneti137605. Legius syndrome.
PharmGKBiPA134897382.
GenAtlasiSearch...

Phylogenomic databases

eggNOGiENOG410IQ20. Eukaryota.
ENOG4112CZ7. LUCA.
GeneTreeiENSGT00730000110272.
HOGENOMiHOG000220886.
HOVERGENiHBG057556.
InParanoidiQ7Z699.
KOiK04703.
OMAiISQGCPA.
OrthoDBiEOG091G0OE8.
PhylomeDBiQ7Z699.
TreeFamiTF321411.

Enzyme and pathway databases

ReactomeiR-HSA-5658442. Regulation of RAS by GAPs.
R-HSA-5658623. FGFRL1 modulation of FGFR1 signaling.
R-HSA-8849471. PTK6 Regulates RHO GTPases, RAS GTPase and MAP kinases.

Miscellaneous databases

ChiTaRSiSPRED1. human.
GeneWikiiSPRED1.
GenomeRNAii161742.
PROiQ7Z699.
SOURCEiSearch...

Gene expression databases

BgeeiENSG00000166068.
CleanExiHS_SPRED1.
ExpressionAtlasiQ7Z699. baseline and differential.
GenevisibleiQ7Z699. HS.

Family and domain databases

Gene3Di2.30.29.30. 1 hit.
InterProiIPR023337. KBD.
IPR011993. PH_dom-like.
IPR007875. Sprouty.
IPR000697. WH1/EVH1_dom.
[Graphical view]
PfamiPF05210. Sprouty. 1 hit.
PF00568. WH1. 1 hit.
[Graphical view]
SMARTiSM00461. WH1. 1 hit.
[Graphical view]
SUPFAMiSSF50729. SSF50729. 1 hit.
PROSITEiPS51488. KBD. 1 hit.
PS51227. SPR. 1 hit.
PS50229. WH1. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiSPRE1_HUMAN
AccessioniPrimary (citable) accession number: Q7Z699
Secondary accession number(s): B2RPJ8, Q05D53, Q8N256
Entry historyi
Integrated into UniProtKB/Swiss-Prot: January 4, 2005
Last sequence update: January 4, 2005
Last modified: November 30, 2016
This is version 126 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. Human chromosome 15
    Human chromosome 15: entries, gene names and cross-references to MIM
  2. Human entries with polymorphisms or disease mutations
    List of human entries with polymorphisms or disease mutations
  3. Human polymorphisms and disease mutations
    Index of human polymorphisms and disease mutations
  4. MIM cross-references
    Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
  5. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  6. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.