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Q7Z698 (SPRE2_HUMAN) Reviewed, UniProtKB/Swiss-Prot

Last modified April 16, 2014. Version 98. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (4) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Alt products·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Sprouty-related, EVH1 domain-containing protein 2

Short name=Spred-2
Gene names
Name:SPRED2
OrganismHomo sapiens (Human) [Reference proteome]
Taxonomic identifier9606 [NCBI]
Taxonomic lineageEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo

Protein attributes

Sequence length418 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Tyrosine kinase substrate that inhibits growth-factor-mediated activation of MAP kinase.

Subunit structure

Interacts with Ras By similarity. Homodimer and heterodimer. Able to interact with SPRED1 to form heterodimers. Ref.1

Subcellular location

Membrane; Peripheral membrane protein; Cytoplasmic side By similarity. Cytoplasmic vesiclesecretory vesicle membrane; Peripheral membrane protein; Cytoplasmic side Potential. Cytoplasm. Note: Especially found in the cytoplasm of the stratum spinosum cells called membrane-coated granules.

Tissue specificity

Expressed in liver, skin, small intestine, salivary gland and prostate. Ref.6

Post-translational modification

Phosphorylated on tyrosine. Phosphorylation of Tyr-228 and Tyr-231 are required for ubiquitination. Ref.7

Ubiquitinated; leading to degradation by the proteasome. Ref.7

Sequence similarities

Contains 1 KBD domain.

Contains 1 SPR (sprouty) domain.

Contains 1 WH1 domain.

Alternative products

This entry describes 2 isoforms produced by alternative splicing. [Align] [Select]
Isoform 1 (identifier: Q7Z698-1)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.
Isoform 2 (identifier: Q7Z698-2)

The sequence of this isoform differs from the canonical sequence as follows:
     1-9: MTEETHPDD → MASPGS
Note: No experimental confirmation available.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 418418Sprouty-related, EVH1 domain-containing protein 2
PRO_0000076910

Regions

Domain5 – 122118WH1
Domain201 – 25757KBD
Domain308 – 416109SPR

Amino acid modifications

Modified residue2281Phosphotyrosine Probable
Modified residue2311Phosphotyrosine Probable

Natural variations

Alternative sequence1 – 99MTEETHPDD → MASPGS in isoform 2.
VSP_043755

Experimental info

Sequence conflict331Q → R in BAH12943. Ref.2
Sequence conflict2021Y → C in AAP59415. Ref.1

Secondary structure

.................... 418
Helix Strand Turn

Details...

Sequences

Sequence LengthMass (Da)Tools
Isoform 1 [UniParc].

Last modified July 25, 2006. Version 2.
Checksum: CA4FE80C45008851

FASTA41847,558
        10         20         30         40         50         60 
MTEETHPDDD SYIVRVKAVV MTRDDSSGGW FPQEGGGISR VGVCKVMHPE GNGRSGFLIH 

        70         80         90        100        110        120 
GERQKDKLVV LECYVRKDLV YTKANPTFHH WKVDNRKFGL TFQSPADARA FDRGVRKAIE 

       130        140        150        160        170        180 
DLIEGSTTSS STIHNEAELG DDDVFTTATD SSSNSSQKRE QPTRTISSPT SCEHRRIYTL 

       190        200        210        220        230        240 
GHLHDSYPTD HYHLDQPMPR PYRQVSFPDD DEEIVRINPR EKIWMTGYED YRHAPVRGKY 

       250        260        270        280        290        300 
PDPSEDADSS YVRFAKGEVP KHDYNYPYVD SSDFGLGEDP KGRGGSVIKT QPSRGKSRRR 

       310        320        330        340        350        360 
KEDGERSRCV YCRDMFNHEE NRRGHCQDAP DSVRTCIRRV SCMWCADSML YHCMSDPEGD 

       370        380        390        400        410 
YTDPCSCDTS DEKFCLRWMA LIALSFLAPC MCCYLPLRAC YHCGVMCRCC GGKHKAAA 

« Hide

Isoform 2 [UniParc].

Checksum: 2E6F7236B9F0FB3C
Show »

FASTA41547,033

References

« Hide 'large scale' references
[1]"Distinct requirements for the Sprouty domain for functional activity of Spred proteins."
King J.A.J., Straffon A.F.L., D'Abaco G.M., Poon C.L.C., I S.T.T., Smith C.M., Buchert M., Corcoran N.M., Hall N.E., Callus B.A., Sarcevic B., Martin D., Lock P., Hovens C.M.
Biochem. J. 388:445-454(2005) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1), INTERACTION WITH SPRED1.
Tissue: Glioblastoma.
[2]"Complete sequencing and characterization of 21,243 full-length human cDNAs."
Ota T., Suzuki Y., Nishikawa T., Otsuki T., Sugiyama T., Irie R., Wakamatsu A., Hayashi K., Sato H., Nagai K., Kimura K., Makita H., Sekine M., Obayashi M., Nishi T., Shibahara T., Tanaka T., Ishii S. expand/collapse author list , Yamamoto J., Saito K., Kawai Y., Isono Y., Nakamura Y., Nagahari K., Murakami K., Yasuda T., Iwayanagi T., Wagatsuma M., Shiratori A., Sudo H., Hosoiri T., Kaku Y., Kodaira H., Kondo H., Sugawara M., Takahashi M., Kanda K., Yokoi T., Furuya T., Kikkawa E., Omura Y., Abe K., Kamihara K., Katsuta N., Sato K., Tanikawa M., Yamazaki M., Ninomiya K., Ishibashi T., Yamashita H., Murakawa K., Fujimori K., Tanai H., Kimata M., Watanabe M., Hiraoka S., Chiba Y., Ishida S., Ono Y., Takiguchi S., Watanabe S., Yosida M., Hotuta T., Kusano J., Kanehori K., Takahashi-Fujii A., Hara H., Tanase T.-O., Nomura Y., Togiya S., Komai F., Hara R., Takeuchi K., Arita M., Imose N., Musashino K., Yuuki H., Oshima A., Sasaki N., Aotsuka S., Yoshikawa Y., Matsunawa H., Ichihara T., Shiohata N., Sano S., Moriya S., Momiyama H., Satoh N., Takami S., Terashima Y., Suzuki O., Nakagawa S., Senoh A., Mizoguchi H., Goto Y., Shimizu F., Wakebe H., Hishigaki H., Watanabe T., Sugiyama A., Takemoto M., Kawakami B., Yamazaki M., Watanabe K., Kumagai A., Itakura S., Fukuzumi Y., Fujimori Y., Komiyama M., Tashiro H., Tanigami A., Fujiwara T., Ono T., Yamada K., Fujii Y., Ozaki K., Hirao M., Ohmori Y., Kawabata A., Hikiji T., Kobatake N., Inagaki H., Ikema Y., Okamoto S., Okitani R., Kawakami T., Noguchi S., Itoh T., Shigeta K., Senba T., Matsumura K., Nakajima Y., Mizuno T., Morinaga M., Sasaki M., Togashi T., Oyama M., Hata H., Watanabe M., Komatsu T., Mizushima-Sugano J., Satoh T., Shirai Y., Takahashi Y., Nakagawa K., Okumura K., Nagase T., Nomura N., Kikuchi H., Masuho Y., Yamashita R., Nakai K., Yada T., Nakamura Y., Ohara O., Isogai T., Sugano S.
Nat. Genet. 36:40-45(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 2).
[3]"Generation and annotation of the DNA sequences of human chromosomes 2 and 4."
Hillier L.W., Graves T.A., Fulton R.S., Fulton L.A., Pepin K.H., Minx P., Wagner-McPherson C., Layman D., Wylie K., Sekhon M., Becker M.C., Fewell G.A., Delehaunty K.D., Miner T.L., Nash W.E., Kremitzki C., Oddy L., Du H. expand/collapse author list , Sun H., Bradshaw-Cordum H., Ali J., Carter J., Cordes M., Harris A., Isak A., van Brunt A., Nguyen C., Du F., Courtney L., Kalicki J., Ozersky P., Abbott S., Armstrong J., Belter E.A., Caruso L., Cedroni M., Cotton M., Davidson T., Desai A., Elliott G., Erb T., Fronick C., Gaige T., Haakenson W., Haglund K., Holmes A., Harkins R., Kim K., Kruchowski S.S., Strong C.M., Grewal N., Goyea E., Hou S., Levy A., Martinka S., Mead K., McLellan M.D., Meyer R., Randall-Maher J., Tomlinson C., Dauphin-Kohlberg S., Kozlowicz-Reilly A., Shah N., Swearengen-Shahid S., Snider J., Strong J.T., Thompson J., Yoakum M., Leonard S., Pearman C., Trani L., Radionenko M., Waligorski J.E., Wang C., Rock S.M., Tin-Wollam A.-M., Maupin R., Latreille P., Wendl M.C., Yang S.-P., Pohl C., Wallis J.W., Spieth J., Bieri T.A., Berkowicz N., Nelson J.O., Osborne J., Ding L., Meyer R., Sabo A., Shotland Y., Sinha P., Wohldmann P.E., Cook L.L., Hickenbotham M.T., Eldred J., Williams D., Jones T.A., She X., Ciccarelli F.D., Izaurralde E., Taylor J., Schmutz J., Myers R.M., Cox D.R., Huang X., McPherson J.D., Mardis E.R., Clifton S.W., Warren W.C., Chinwalla A.T., Eddy S.R., Marra M.A., Ovcharenko I., Furey T.S., Miller W., Eichler E.E., Bork P., Suyama M., Torrents D., Waterston R.H., Wilson R.K.
Nature 434:724-731(2005) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[4]Mural R.J., Istrail S., Sutton G.G., Florea L., Halpern A.L., Mobarry C.M., Lippert R., Walenz B., Shatkay H., Dew I., Miller J.R., Flanigan M.J., Edwards N.J., Bolanos R., Fasulo D., Halldorsson B.V., Hannenhalli S., Turner R. expand/collapse author list , Yooseph S., Lu F., Nusskern D.R., Shue B.C., Zheng X.H., Zhong F., Delcher A.L., Huson D.H., Kravitz S.A., Mouchard L., Reinert K., Remington K.A., Clark A.G., Waterman M.S., Eichler E.E., Adams M.D., Hunkapiller M.W., Myers E.W., Venter J.C.
Submitted (SEP-2005) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[5]"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
The MGC Project Team
Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
Tissue: Brain and Ovary.
[6]"Expression and subcellular localization of Spred proteins in mouse and human tissues."
Engelhardt C.M., Bundschu K., Messerschmitt M., Renne T., Walter U., Reinhard M., Schuh K.
Histochem. Cell Biol. 122:527-538(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: TISSUE SPECIFICITY.
[7]"Spred-2 steady-state levels are regulated by phosphorylation and Cbl-mediated ubiquitination."
Lock P., I S.T.T., Straffon A.F., Schieb H., Hovens C.M., Stylli S.S.
Biochem. Biophys. Res. Commun. 351:1018-1023(2006) [PubMed] [Europe PMC] [Abstract]
Cited for: UBIQUITINATION, PHOSPHORYLATION AT TYR-228 AND TYR-231.
[8]"1H, 13C and 15N resonance assignment of the human Spred2 EVH1 domain."
Zimmermann J., Jarchau T., Walter U., Oschkinat H., Ball L.J.
J. Biomol. NMR 29:435-436(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: STRUCTURE BY NMR OF 1-124.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AY299090 mRNA. Translation: AAP59415.1.
AK299079 mRNA. Translation: BAH12943.1.
AC012370 Genomic DNA. No translation available.
AC097503 Genomic DNA. No translation available.
CH471053 Genomic DNA. Translation: EAW99905.1.
CH471053 Genomic DNA. Translation: EAW99906.1.
BC111495 mRNA. Translation: AAI11496.1.
BC130292 mRNA. Translation: AAI30293.1.
BC136334 mRNA. Translation: AAI36335.1.
RefSeqNP_001121682.1. NM_001128210.1.
NP_861449.2. NM_181784.2.
UniGeneHs.59332.

3D structure databases

PDBe
RCSB PDB
PDBj
EntryMethodResolution (Å)ChainPositionsPDBsum
2JP2NMR-A1-124[»]
ProteinModelPortalQ7Z698.
SMRQ7Z698. Positions 1-124.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

BioGrid128344. 5 interactions.
IntActQ7Z698. 1 interaction.
MINTMINT-8020116.
STRING9606.ENSP00000348753.

PTM databases

PhosphoSiteQ7Z698.

Polymorphism databases

DMDM110825745.

Proteomic databases

PaxDbQ7Z698.
PRIDEQ7Z698.

Protocols and materials databases

DNASU200734.
StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblENST00000356388; ENSP00000348753; ENSG00000198369. [Q7Z698-1]
ENST00000443619; ENSP00000393697; ENSG00000198369. [Q7Z698-2]
GeneID200734.
KEGGhsa:200734.
UCSCuc002sdr.4. human. [Q7Z698-1]
uc010fcw.3. human. [Q7Z698-2]

Organism-specific databases

CTD200734.
GeneCardsGC02M065537.
HGNCHGNC:17722. SPRED2.
HPACAB037223.
HPA038256.
MIM609292. gene.
neXtProtNX_Q7Z698.
PharmGKBPA134956365.
GenAtlasSearch...

Phylogenomic databases

eggNOGNOG277322.
HOGENOMHOG000220886.
HOVERGENHBG057556.
InParanoidQ7Z698.
KOK04703.
OrthoDBEOG7T7GV7.
PhylomeDBQ7Z698.
TreeFamTF321411.

Gene expression databases

ArrayExpressQ7Z698.
BgeeQ7Z698.
CleanExHS_SPRED2.
GenevestigatorQ7Z698.

Family and domain databases

Gene3D2.30.29.30. 1 hit.
InterProIPR023337. KBD.
IPR011993. PH_like_dom.
IPR007875. Sprouty.
IPR000697. WH1/EVH1.
[Graphical view]
PfamPF05210. Sprouty. 1 hit.
PF00568. WH1. 1 hit.
[Graphical view]
PROSITEPS51488. KBD. 1 hit.
PS51227. SPR. 1 hit.
PS50229. WH1. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

ChiTaRSSPRED2. human.
EvolutionaryTraceQ7Z698.
GeneWikiSPRED2.
GenomeRNAi200734.
NextBio89969.
PROQ7Z698.
SOURCESearch...

Entry information

Entry nameSPRE2_HUMAN
AccessionPrimary (citable) accession number: Q7Z698
Secondary accession number(s): A1L3V4 expand/collapse secondary AC list , B7Z5K7, D6W5F7, E9PEP0, Q2NKX6
Entry history
Integrated into UniProtKB/Swiss-Prot: January 4, 2005
Last sequence update: July 25, 2006
Last modified: April 16, 2014
This is version 98 of the entry and version 2 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Relevant documents

SIMILARITY comments

Index of protein domains and families

PDB cross-references

Index of Protein Data Bank (PDB) cross-references

MIM cross-references

Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot

Human chromosome 2

Human chromosome 2: entries, gene names and cross-references to MIM