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Q7Z698

- SPRE2_HUMAN

UniProt

Q7Z698 - SPRE2_HUMAN

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Protein

Sprouty-related, EVH1 domain-containing protein 2

Gene

SPRED2

Organism
Homo sapiens (Human)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli

Functioni

Tyrosine kinase substrate that inhibits growth-factor-mediated activation of MAP kinase.

GO - Molecular functioni

  1. protein kinase binding Source: BHF-UCL
  2. protein serine/threonine kinase inhibitor activity Source: BHF-UCL
  3. stem cell factor receptor binding Source: UniProtKB

GO - Biological processi

  1. inactivation of MAPK activity Source: UniProtKB
  2. multicellular organismal development Source: InterPro
  3. negative regulation of peptidyl-threonine phosphorylation Source: BHF-UCL
  4. positive regulation of DNA damage response, signal transduction by p53 class mediator Source: BHF-UCL
  5. regulation of protein deacetylation Source: BHF-UCL
Complete GO annotation...

Names & Taxonomyi

Protein namesi
Recommended name:
Sprouty-related, EVH1 domain-containing protein 2
Short name:
Spred-2
Gene namesi
Name:SPRED2
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
ProteomesiUP000005640: Chromosome 2

Organism-specific databases

HGNCiHGNC:17722. SPRED2.

Subcellular locationi

Membrane By similarity; Peripheral membrane protein By similarity; Cytoplasmic side By similarity. Cytoplasmic vesiclesecretory vesicle membrane Curated; Peripheral membrane protein Curated; Cytoplasmic side Curated. Cytoplasm
Note: Especially found in the cytoplasm of the stratum spinosum cells called membrane-coated granules.

GO - Cellular componenti

  1. cytoplasm Source: HPA
  2. cytoplasmic vesicle Source: UniProtKB-KW
  3. nucleus Source: HPA
  4. plasma membrane Source: Ensembl
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm, Cytoplasmic vesicle, Membrane

Pathology & Biotechi

Organism-specific databases

PharmGKBiPA134956365.

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 418418Sprouty-related, EVH1 domain-containing protein 2PRO_0000076910Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei228 – 2281Phosphotyrosine1 Publication
Modified residuei231 – 2311Phosphotyrosine1 Publication

Post-translational modificationi

Phosphorylated on tyrosine. Phosphorylation of Tyr-228 and Tyr-231 are required for ubiquitination.1 Publication
Ubiquitinated; leading to degradation by the proteasome.1 Publication

Keywords - PTMi

Phosphoprotein, Ubl conjugation

Proteomic databases

MaxQBiQ7Z698.
PaxDbiQ7Z698.
PRIDEiQ7Z698.

PTM databases

PhosphoSiteiQ7Z698.

Expressioni

Tissue specificityi

Expressed in liver, skin, small intestine, salivary gland and prostate.1 Publication

Gene expression databases

BgeeiQ7Z698.
CleanExiHS_SPRED2.
ExpressionAtlasiQ7Z698. baseline and differential.
GenevestigatoriQ7Z698.

Organism-specific databases

HPAiCAB037223.
HPA038256.

Interactioni

Subunit structurei

Interacts with Ras (By similarity). Homodimer and heterodimer. Able to interact with SPRED1 to form heterodimers.By similarity1 Publication

Binary interactionsi

WithEntry#Exp.IntActNotes
ZDHHC17Q8IUH53EBI-7082156,EBI-524753

Protein-protein interaction databases

BioGridi128344. 5 interactions.
IntActiQ7Z698. 2 interactions.
MINTiMINT-8020116.
STRINGi9606.ENSP00000348753.

Structurei

Secondary structure

1
418
Legend: HelixTurnBeta strand
Show more details
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Beta strandi5 – 84Combined sources
Beta strandi11 – 2313Combined sources
Beta strandi27 – 326Combined sources
Beta strandi39 – 446Combined sources
Beta strandi57 – 6711Combined sources
Beta strandi69 – 779Combined sources
Beta strandi81 – 855Combined sources
Beta strandi88 – 925Combined sources
Beta strandi94 – 10411Combined sources
Helixi105 – 12319Combined sources

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
2JP2NMR-A1-124[»]
ProteinModelPortaliQ7Z698.
SMRiQ7Z698. Positions 1-124.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiQ7Z698.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini5 – 122118WH1PROSITE-ProRule annotationAdd
BLAST
Domaini201 – 25757KBDPROSITE-ProRule annotationAdd
BLAST
Domaini308 – 416109SPRPROSITE-ProRule annotationAdd
BLAST

Sequence similaritiesi

Contains 1 KBD domain.PROSITE-ProRule annotation
Contains 1 SPR (sprouty) domain.PROSITE-ProRule annotation
Contains 1 WH1 domain.PROSITE-ProRule annotation

Phylogenomic databases

eggNOGiNOG277322.
GeneTreeiENSGT00730000110272.
HOGENOMiHOG000220886.
HOVERGENiHBG057556.
InParanoidiQ7Z698.
KOiK04703.
OrthoDBiEOG7T7GV7.
PhylomeDBiQ7Z698.
TreeFamiTF321411.

Family and domain databases

Gene3Di2.30.29.30. 1 hit.
InterProiIPR023337. KBD.
IPR011993. PH_like_dom.
IPR007875. Sprouty.
IPR000697. WH1/EVH1.
[Graphical view]
PfamiPF05210. Sprouty. 1 hit.
PF00568. WH1. 1 hit.
[Graphical view]
PROSITEiPS51488. KBD. 1 hit.
PS51227. SPR. 1 hit.
PS50229. WH1. 1 hit.
[Graphical view]

Sequences (2)i

Sequence statusi: Complete.

This entry describes 2 isoformsi produced by alternative splicing. Align

Isoform 1 (identifier: Q7Z698-1) [UniParc]FASTAAdd to Basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MTEETHPDDD SYIVRVKAVV MTRDDSSGGW FPQEGGGISR VGVCKVMHPE
60 70 80 90 100
GNGRSGFLIH GERQKDKLVV LECYVRKDLV YTKANPTFHH WKVDNRKFGL
110 120 130 140 150
TFQSPADARA FDRGVRKAIE DLIEGSTTSS STIHNEAELG DDDVFTTATD
160 170 180 190 200
SSSNSSQKRE QPTRTISSPT SCEHRRIYTL GHLHDSYPTD HYHLDQPMPR
210 220 230 240 250
PYRQVSFPDD DEEIVRINPR EKIWMTGYED YRHAPVRGKY PDPSEDADSS
260 270 280 290 300
YVRFAKGEVP KHDYNYPYVD SSDFGLGEDP KGRGGSVIKT QPSRGKSRRR
310 320 330 340 350
KEDGERSRCV YCRDMFNHEE NRRGHCQDAP DSVRTCIRRV SCMWCADSML
360 370 380 390 400
YHCMSDPEGD YTDPCSCDTS DEKFCLRWMA LIALSFLAPC MCCYLPLRAC
410
YHCGVMCRCC GGKHKAAA
Length:418
Mass (Da):47,558
Last modified:July 25, 2006 - v2
Checksum:iCA4FE80C45008851
GO
Isoform 2 (identifier: Q7Z698-2) [UniParc]FASTAAdd to Basket

The sequence of this isoform differs from the canonical sequence as follows:
     1-9: MTEETHPDD → MASPGS

Note: No experimental confirmation available.

Show »
Length:415
Mass (Da):47,033
Checksum:i2E6F7236B9F0FB3C
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti33 – 331Q → R in BAH12943. (PubMed:14702039)Curated
Sequence conflicti202 – 2021Y → C in AAP59415. (PubMed:15683364)Curated

Alternative sequence

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Alternative sequencei1 – 99MTEETHPDD → MASPGS in isoform 2. 1 PublicationVSP_043755

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AY299090 mRNA. Translation: AAP59415.1.
AK299079 mRNA. Translation: BAH12943.1.
AC012370 Genomic DNA. No translation available.
AC097503 Genomic DNA. No translation available.
CH471053 Genomic DNA. Translation: EAW99905.1.
CH471053 Genomic DNA. Translation: EAW99906.1.
BC111495 mRNA. Translation: AAI11496.1.
BC130292 mRNA. Translation: AAI30293.1.
BC136334 mRNA. Translation: AAI36335.1.
CCDSiCCDS33211.1. [Q7Z698-1]
CCDS46308.1. [Q7Z698-2]
RefSeqiNP_001121682.1. NM_001128210.1. [Q7Z698-2]
NP_861449.2. NM_181784.2. [Q7Z698-1]
XP_006712029.1. XM_006711966.1. [Q7Z698-1]
UniGeneiHs.59332.

Genome annotation databases

EnsembliENST00000356388; ENSP00000348753; ENSG00000198369. [Q7Z698-1]
ENST00000443619; ENSP00000393697; ENSG00000198369. [Q7Z698-2]
GeneIDi200734.
KEGGihsa:200734.
UCSCiuc002sdr.4. human. [Q7Z698-1]
uc010fcw.3. human. [Q7Z698-2]

Polymorphism databases

DMDMi110825745.

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AY299090 mRNA. Translation: AAP59415.1 .
AK299079 mRNA. Translation: BAH12943.1 .
AC012370 Genomic DNA. No translation available.
AC097503 Genomic DNA. No translation available.
CH471053 Genomic DNA. Translation: EAW99905.1 .
CH471053 Genomic DNA. Translation: EAW99906.1 .
BC111495 mRNA. Translation: AAI11496.1 .
BC130292 mRNA. Translation: AAI30293.1 .
BC136334 mRNA. Translation: AAI36335.1 .
CCDSi CCDS33211.1. [Q7Z698-1 ]
CCDS46308.1. [Q7Z698-2 ]
RefSeqi NP_001121682.1. NM_001128210.1. [Q7Z698-2 ]
NP_861449.2. NM_181784.2. [Q7Z698-1 ]
XP_006712029.1. XM_006711966.1. [Q7Z698-1 ]
UniGenei Hs.59332.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
Entry Method Resolution (Å) Chain Positions PDBsum
2JP2 NMR - A 1-124 [» ]
ProteinModelPortali Q7Z698.
SMRi Q7Z698. Positions 1-124.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

BioGridi 128344. 5 interactions.
IntActi Q7Z698. 2 interactions.
MINTi MINT-8020116.
STRINGi 9606.ENSP00000348753.

PTM databases

PhosphoSitei Q7Z698.

Polymorphism databases

DMDMi 110825745.

Proteomic databases

MaxQBi Q7Z698.
PaxDbi Q7Z698.
PRIDEi Q7Z698.

Protocols and materials databases

DNASUi 200734.
Structural Biology Knowledgebase Search...

Genome annotation databases

Ensembli ENST00000356388 ; ENSP00000348753 ; ENSG00000198369 . [Q7Z698-1 ]
ENST00000443619 ; ENSP00000393697 ; ENSG00000198369 . [Q7Z698-2 ]
GeneIDi 200734.
KEGGi hsa:200734.
UCSCi uc002sdr.4. human. [Q7Z698-1 ]
uc010fcw.3. human. [Q7Z698-2 ]

Organism-specific databases

CTDi 200734.
GeneCardsi GC02M065537.
HGNCi HGNC:17722. SPRED2.
HPAi CAB037223.
HPA038256.
MIMi 609292. gene.
neXtProti NX_Q7Z698.
PharmGKBi PA134956365.
GenAtlasi Search...

Phylogenomic databases

eggNOGi NOG277322.
GeneTreei ENSGT00730000110272.
HOGENOMi HOG000220886.
HOVERGENi HBG057556.
InParanoidi Q7Z698.
KOi K04703.
OrthoDBi EOG7T7GV7.
PhylomeDBi Q7Z698.
TreeFami TF321411.

Miscellaneous databases

ChiTaRSi SPRED2. human.
EvolutionaryTracei Q7Z698.
GeneWikii SPRED2.
GenomeRNAii 200734.
NextBioi 89969.
PROi Q7Z698.
SOURCEi Search...

Gene expression databases

Bgeei Q7Z698.
CleanExi HS_SPRED2.
ExpressionAtlasi Q7Z698. baseline and differential.
Genevestigatori Q7Z698.

Family and domain databases

Gene3Di 2.30.29.30. 1 hit.
InterProi IPR023337. KBD.
IPR011993. PH_like_dom.
IPR007875. Sprouty.
IPR000697. WH1/EVH1.
[Graphical view ]
Pfami PF05210. Sprouty. 1 hit.
PF00568. WH1. 1 hit.
[Graphical view ]
PROSITEi PS51488. KBD. 1 hit.
PS51227. SPR. 1 hit.
PS50229. WH1. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1), INTERACTION WITH SPRED1.
    Tissue: Glioblastoma.
  2. "Complete sequencing and characterization of 21,243 full-length human cDNAs."
    Ota T., Suzuki Y., Nishikawa T., Otsuki T., Sugiyama T., Irie R., Wakamatsu A., Hayashi K., Sato H., Nagai K., Kimura K., Makita H., Sekine M., Obayashi M., Nishi T., Shibahara T., Tanaka T., Ishii S.
    , Yamamoto J., Saito K., Kawai Y., Isono Y., Nakamura Y., Nagahari K., Murakami K., Yasuda T., Iwayanagi T., Wagatsuma M., Shiratori A., Sudo H., Hosoiri T., Kaku Y., Kodaira H., Kondo H., Sugawara M., Takahashi M., Kanda K., Yokoi T., Furuya T., Kikkawa E., Omura Y., Abe K., Kamihara K., Katsuta N., Sato K., Tanikawa M., Yamazaki M., Ninomiya K., Ishibashi T., Yamashita H., Murakawa K., Fujimori K., Tanai H., Kimata M., Watanabe M., Hiraoka S., Chiba Y., Ishida S., Ono Y., Takiguchi S., Watanabe S., Yosida M., Hotuta T., Kusano J., Kanehori K., Takahashi-Fujii A., Hara H., Tanase T.-O., Nomura Y., Togiya S., Komai F., Hara R., Takeuchi K., Arita M., Imose N., Musashino K., Yuuki H., Oshima A., Sasaki N., Aotsuka S., Yoshikawa Y., Matsunawa H., Ichihara T., Shiohata N., Sano S., Moriya S., Momiyama H., Satoh N., Takami S., Terashima Y., Suzuki O., Nakagawa S., Senoh A., Mizoguchi H., Goto Y., Shimizu F., Wakebe H., Hishigaki H., Watanabe T., Sugiyama A., Takemoto M., Kawakami B., Yamazaki M., Watanabe K., Kumagai A., Itakura S., Fukuzumi Y., Fujimori Y., Komiyama M., Tashiro H., Tanigami A., Fujiwara T., Ono T., Yamada K., Fujii Y., Ozaki K., Hirao M., Ohmori Y., Kawabata A., Hikiji T., Kobatake N., Inagaki H., Ikema Y., Okamoto S., Okitani R., Kawakami T., Noguchi S., Itoh T., Shigeta K., Senba T., Matsumura K., Nakajima Y., Mizuno T., Morinaga M., Sasaki M., Togashi T., Oyama M., Hata H., Watanabe M., Komatsu T., Mizushima-Sugano J., Satoh T., Shirai Y., Takahashi Y., Nakagawa K., Okumura K., Nagase T., Nomura N., Kikuchi H., Masuho Y., Yamashita R., Nakai K., Yada T., Nakamura Y., Ohara O., Isogai T., Sugano S.
    Nat. Genet. 36:40-45(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 2).
  3. "Generation and annotation of the DNA sequences of human chromosomes 2 and 4."
    Hillier L.W., Graves T.A., Fulton R.S., Fulton L.A., Pepin K.H., Minx P., Wagner-McPherson C., Layman D., Wylie K., Sekhon M., Becker M.C., Fewell G.A., Delehaunty K.D., Miner T.L., Nash W.E., Kremitzki C., Oddy L., Du H.
    , Sun H., Bradshaw-Cordum H., Ali J., Carter J., Cordes M., Harris A., Isak A., van Brunt A., Nguyen C., Du F., Courtney L., Kalicki J., Ozersky P., Abbott S., Armstrong J., Belter E.A., Caruso L., Cedroni M., Cotton M., Davidson T., Desai A., Elliott G., Erb T., Fronick C., Gaige T., Haakenson W., Haglund K., Holmes A., Harkins R., Kim K., Kruchowski S.S., Strong C.M., Grewal N., Goyea E., Hou S., Levy A., Martinka S., Mead K., McLellan M.D., Meyer R., Randall-Maher J., Tomlinson C., Dauphin-Kohlberg S., Kozlowicz-Reilly A., Shah N., Swearengen-Shahid S., Snider J., Strong J.T., Thompson J., Yoakum M., Leonard S., Pearman C., Trani L., Radionenko M., Waligorski J.E., Wang C., Rock S.M., Tin-Wollam A.-M., Maupin R., Latreille P., Wendl M.C., Yang S.-P., Pohl C., Wallis J.W., Spieth J., Bieri T.A., Berkowicz N., Nelson J.O., Osborne J., Ding L., Meyer R., Sabo A., Shotland Y., Sinha P., Wohldmann P.E., Cook L.L., Hickenbotham M.T., Eldred J., Williams D., Jones T.A., She X., Ciccarelli F.D., Izaurralde E., Taylor J., Schmutz J., Myers R.M., Cox D.R., Huang X., McPherson J.D., Mardis E.R., Clifton S.W., Warren W.C., Chinwalla A.T., Eddy S.R., Marra M.A., Ovcharenko I., Furey T.S., Miller W., Eichler E.E., Bork P., Suyama M., Torrents D., Waterston R.H., Wilson R.K.
    Nature 434:724-731(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
  4. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
  5. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
    Tissue: Brain and Ovary.
  6. "Expression and subcellular localization of Spred proteins in mouse and human tissues."
    Engelhardt C.M., Bundschu K., Messerschmitt M., Renne T., Walter U., Reinhard M., Schuh K.
    Histochem. Cell Biol. 122:527-538(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: TISSUE SPECIFICITY.
  7. "Spred-2 steady-state levels are regulated by phosphorylation and Cbl-mediated ubiquitination."
    Lock P., I S.T.T., Straffon A.F., Schieb H., Hovens C.M., Stylli S.S.
    Biochem. Biophys. Res. Commun. 351:1018-1023(2006) [PubMed] [Europe PMC] [Abstract]
    Cited for: UBIQUITINATION, PHOSPHORYLATION AT TYR-228 AND TYR-231.
  8. "1H, 13C and 15N resonance assignment of the human Spred2 EVH1 domain."
    Zimmermann J., Jarchau T., Walter U., Oschkinat H., Ball L.J.
    J. Biomol. NMR 29:435-436(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: STRUCTURE BY NMR OF 1-124.

Entry informationi

Entry nameiSPRE2_HUMAN
AccessioniPrimary (citable) accession number: Q7Z698
Secondary accession number(s): A1L3V4
, B7Z5K7, D6W5F7, E9PEP0, Q2NKX6
Entry historyi
Integrated into UniProtKB/Swiss-Prot: January 4, 2005
Last sequence update: July 25, 2006
Last modified: November 26, 2014
This is version 104 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. Human chromosome 2
    Human chromosome 2: entries, gene names and cross-references to MIM
  2. MIM cross-references
    Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
  3. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  4. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3