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Protein

Calmodulin-lysine N-methyltransferase

Gene

CAMKMT

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Catalyzes the trimethylation of 'Lys-116' in calmodulin.1 Publication

Catalytic activityi

S-adenosyl-L-methionine + calmodulin L-lysine = S-adenosyl-L-homocysteine + calmodulin N(6)-methyl-L-lysine.PROSITE-ProRule annotation1 Publication

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Methyltransferase, Transferase

Keywords - Ligandi

S-adenosyl-L-methionine

Enzyme and pathway databases

ReactomeiR-HSA-2514859. Inactivation, recovery and regulation of the phototransduction cascade.

Names & Taxonomyi

Protein namesi
Recommended name:
Calmodulin-lysine N-methyltransferase (EC:2.1.1.60)
Short name:
CLNMT
Short name:
CaM KMT
Gene namesi
Name:CAMKMT
Synonyms:C2orf34, CLNMT
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
Proteomesi
  • UP000005640 Componenti: Chromosome 2

Organism-specific databases

HGNCiHGNC:26276. CAMKMT.

Subcellular locationi

Isoform 1 :
Isoform 2 :

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm, Golgi apparatus, Nucleus

Pathology & Biotechi

Organism-specific databases

MalaCardsiCAMKMT.
Orphaneti163693. 2p21 microdeletion syndrome.
369881. 2p21 microdeletion syndrome without cystinuria.
238523. Atypical hypotonia - cystinuria syndrome.
PharmGKBiPA142672380.

Polymorphism and mutation databases

DMDMi158563944.

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 323323Calmodulin-lysine N-methyltransferasePRO_0000300115Add
BLAST

Proteomic databases

EPDiQ7Z624.
MaxQBiQ7Z624.
PaxDbiQ7Z624.
PRIDEiQ7Z624.

PTM databases

iPTMnetiQ7Z624.
PhosphoSiteiQ7Z624.

Expressioni

Tissue specificityi

Isoform 1 is expressed in brain,liver, muscle colon and lung. Isoform 2 is expressed in colon, testis, kidney and brain. Isoform 1 and isoform 2 are expressed in normal lymphoblastoid cells but not in lymphoblastoid cells from patients with hypotonia-cystinuria syndrome.1 Publication

Gene expression databases

BgeeiQ7Z624.
CleanExiHS_C2orf34.
ExpressionAtlasiQ7Z624. baseline and differential.
GenevisibleiQ7Z624. HS.

Interactioni

Subunit structurei

Monomer (By similarity). Interacts with HSP90, probably as a client.By similarity1 Publication

Protein-protein interaction databases

BioGridi122917. 28 interactions.
STRINGi9606.ENSP00000367755.

Structurei

Secondary structure

1
323
Legend: HelixTurnBeta strand
Show more details
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Beta strandi71 – 755Combined sources
Beta strandi88 – 958Combined sources
Beta strandi100 – 1045Combined sources
Helixi112 – 1176Combined sources
Helixi129 – 14012Combined sources
Helixi142 – 1454Combined sources
Beta strandi149 – 1546Combined sources
Turni156 – 1583Combined sources
Helixi160 – 1689Combined sources
Beta strandi172 – 1798Combined sources
Helixi181 – 19616Combined sources
Beta strandi205 – 2095Combined sources
Helixi215 – 2195Combined sources
Turni220 – 2234Combined sources
Beta strandi225 – 2328Combined sources
Helixi237 – 2393Combined sources
Helixi240 – 25011Combined sources
Beta strandi251 – 26111Combined sources
Turni264 – 2663Combined sources
Helixi267 – 27711Combined sources
Beta strandi280 – 2867Combined sources
Helixi290 – 30213Combined sources
Turni304 – 3063Combined sources
Helixi309 – 3124Combined sources
Beta strandi315 – 3217Combined sources

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
4PWYX-ray1.90A61-323[»]
ProteinModelPortaliQ7Z624.
SMRiQ7Z624. Positions 61-322.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the class I-like SAM-binding methyltransferase superfamily. CLNMT methyltransferase family.PROSITE-ProRule annotation

Phylogenomic databases

eggNOGiKOG3201. Eukaryota.
ENOG4110DEX. LUCA.
GeneTreeiENSGT00390000002168.
HOVERGENiHBG105701.
InParanoidiQ7Z624.
KOiK18826.
OMAiMLAKYAT.
PhylomeDBiQ7Z624.
TreeFamiTF316589.

Family and domain databases

Gene3Di3.40.50.150. 1 hit.
InterProiIPR025800. CaM-Lys-N-MeTrfase.
IPR019410. Methyltransf_16.
IPR029063. SAM-dependent_MTases.
[Graphical view]
PfamiPF10294. Methyltransf_16. 1 hit.
[Graphical view]
SUPFAMiSSF53335. SSF53335. 1 hit.
PROSITEiPS51610. SAM_CLNMT. 1 hit.
[Graphical view]

Sequences (2)i

Sequence statusi: Complete.

This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 1 (identifier: Q7Z624-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MESRVADAGT GETARAAGGS PAVGCTTRGP VVSAPLGAAR WKLLRQVLKQ
60 70 80 90 100
KHLDDCLRHV SVRRFESFNL FSVTEGKERE TEEEVGAWVQ YTSIFCPEYS
110 120 130 140 150
ISLRHNSGSL NVEDVLTSFD NTGNVCIWPS EEVLAYYCLK HNNIFRALAV
160 170 180 190 200
CELGGGMTCL AGLMVAISAD VKEVLLTDGN EKAIRNVQDI ITRNQKAGVF
210 220 230 240 250
KTQKISSCVL RWDNETDVSQ LEGHFDIVMC ADCLFLDQYR ASLVDAIKRL
260 270 280 290 300
LQPRGKAMVF APRRGNTLNQ FCNLAEKAGF CIQRHENYDE HISNFHSKLK
310 320
KENPDIYEEN LHYPLLLILT KHG
Length:323
Mass (Da):36,128
Last modified:September 11, 2007 - v2
Checksum:i0B2C0D4E2C7B10FB
GO
Isoform 2 (identifier: Q7Z624-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     126-132: CIWPSEE → WEATRGK
     133-323: Missing.

Note: No experimental confirmation available.
Show »
Length:132
Mass (Da):14,487
Checksum:i654B4974ABB574B5
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti209 – 2091V → F in AAH53733 (PubMed:15489334).Curated

Alternative sequence

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Alternative sequencei126 – 1327CIWPSEE → WEATRGK in isoform 2. 1 PublicationVSP_027784
Alternative sequencei133 – 323191Missing in isoform 2. 1 PublicationVSP_027785Add
BLAST

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AK027104 mRNA. Translation: BAB15658.1.
AC016703 Genomic DNA. Translation: AAX93163.1.
AC067957 Genomic DNA. Translation: AAX88866.1.
BC029359 mRNA. Translation: AAH29359.1.
BC053733 mRNA. Translation: AAH53733.1.
CCDSiCCDS1820.1. [Q7Z624-1]
RefSeqiNP_079042.1. NM_024766.4. [Q7Z624-1]
UniGeneiHs.468349.

Genome annotation databases

EnsembliENST00000378494; ENSP00000367755; ENSG00000143919. [Q7Z624-1]
ENST00000403853; ENSP00000385124; ENSG00000143919. [Q7Z624-2]
GeneIDi79823.
KEGGihsa:79823.
UCSCiuc002rul.3. human. [Q7Z624-1]

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AK027104 mRNA. Translation: BAB15658.1.
AC016703 Genomic DNA. Translation: AAX93163.1.
AC067957 Genomic DNA. Translation: AAX88866.1.
BC029359 mRNA. Translation: AAH29359.1.
BC053733 mRNA. Translation: AAH53733.1.
CCDSiCCDS1820.1. [Q7Z624-1]
RefSeqiNP_079042.1. NM_024766.4. [Q7Z624-1]
UniGeneiHs.468349.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
4PWYX-ray1.90A61-323[»]
ProteinModelPortaliQ7Z624.
SMRiQ7Z624. Positions 61-322.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi122917. 28 interactions.
STRINGi9606.ENSP00000367755.

PTM databases

iPTMnetiQ7Z624.
PhosphoSiteiQ7Z624.

Polymorphism and mutation databases

DMDMi158563944.

Proteomic databases

EPDiQ7Z624.
MaxQBiQ7Z624.
PaxDbiQ7Z624.
PRIDEiQ7Z624.

Protocols and materials databases

DNASUi79823.
Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENST00000378494; ENSP00000367755; ENSG00000143919. [Q7Z624-1]
ENST00000403853; ENSP00000385124; ENSG00000143919. [Q7Z624-2]
GeneIDi79823.
KEGGihsa:79823.
UCSCiuc002rul.3. human. [Q7Z624-1]

Organism-specific databases

CTDi79823.
GeneCardsiCAMKMT.
HGNCiHGNC:26276. CAMKMT.
MalaCardsiCAMKMT.
MIMi609559. gene.
neXtProtiNX_Q7Z624.
Orphaneti163693. 2p21 microdeletion syndrome.
369881. 2p21 microdeletion syndrome without cystinuria.
238523. Atypical hypotonia - cystinuria syndrome.
PharmGKBiPA142672380.
GenAtlasiSearch...

Phylogenomic databases

eggNOGiKOG3201. Eukaryota.
ENOG4110DEX. LUCA.
GeneTreeiENSGT00390000002168.
HOVERGENiHBG105701.
InParanoidiQ7Z624.
KOiK18826.
OMAiMLAKYAT.
PhylomeDBiQ7Z624.
TreeFamiTF316589.

Enzyme and pathway databases

ReactomeiR-HSA-2514859. Inactivation, recovery and regulation of the phototransduction cascade.

Miscellaneous databases

GenomeRNAii79823.
PROiQ7Z624.
SOURCEiSearch...

Gene expression databases

BgeeiQ7Z624.
CleanExiHS_C2orf34.
ExpressionAtlasiQ7Z624. baseline and differential.
GenevisibleiQ7Z624. HS.

Family and domain databases

Gene3Di3.40.50.150. 1 hit.
InterProiIPR025800. CaM-Lys-N-MeTrfase.
IPR019410. Methyltransf_16.
IPR029063. SAM-dependent_MTases.
[Graphical view]
PfamiPF10294. Methyltransf_16. 1 hit.
[Graphical view]
SUPFAMiSSF53335. SSF53335. 1 hit.
PROSITEiPS51610. SAM_CLNMT. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "Complete sequencing and characterization of 21,243 full-length human cDNAs."
    Ota T., Suzuki Y., Nishikawa T., Otsuki T., Sugiyama T., Irie R., Wakamatsu A., Hayashi K., Sato H., Nagai K., Kimura K., Makita H., Sekine M., Obayashi M., Nishi T., Shibahara T., Tanaka T., Ishii S.
    , Yamamoto J., Saito K., Kawai Y., Isono Y., Nakamura Y., Nagahari K., Murakami K., Yasuda T., Iwayanagi T., Wagatsuma M., Shiratori A., Sudo H., Hosoiri T., Kaku Y., Kodaira H., Kondo H., Sugawara M., Takahashi M., Kanda K., Yokoi T., Furuya T., Kikkawa E., Omura Y., Abe K., Kamihara K., Katsuta N., Sato K., Tanikawa M., Yamazaki M., Ninomiya K., Ishibashi T., Yamashita H., Murakawa K., Fujimori K., Tanai H., Kimata M., Watanabe M., Hiraoka S., Chiba Y., Ishida S., Ono Y., Takiguchi S., Watanabe S., Yosida M., Hotuta T., Kusano J., Kanehori K., Takahashi-Fujii A., Hara H., Tanase T.-O., Nomura Y., Togiya S., Komai F., Hara R., Takeuchi K., Arita M., Imose N., Musashino K., Yuuki H., Oshima A., Sasaki N., Aotsuka S., Yoshikawa Y., Matsunawa H., Ichihara T., Shiohata N., Sano S., Moriya S., Momiyama H., Satoh N., Takami S., Terashima Y., Suzuki O., Nakagawa S., Senoh A., Mizoguchi H., Goto Y., Shimizu F., Wakebe H., Hishigaki H., Watanabe T., Sugiyama A., Takemoto M., Kawakami B., Yamazaki M., Watanabe K., Kumagai A., Itakura S., Fukuzumi Y., Fujimori Y., Komiyama M., Tashiro H., Tanigami A., Fujiwara T., Ono T., Yamada K., Fujii Y., Ozaki K., Hirao M., Ohmori Y., Kawabata A., Hikiji T., Kobatake N., Inagaki H., Ikema Y., Okamoto S., Okitani R., Kawakami T., Noguchi S., Itoh T., Shigeta K., Senba T., Matsumura K., Nakajima Y., Mizuno T., Morinaga M., Sasaki M., Togashi T., Oyama M., Hata H., Watanabe M., Komatsu T., Mizushima-Sugano J., Satoh T., Shirai Y., Takahashi Y., Nakagawa K., Okumura K., Nagase T., Nomura N., Kikuchi H., Masuho Y., Yamashita R., Nakai K., Yada T., Nakamura Y., Ohara O., Isogai T., Sugano S.
    Nat. Genet. 36:40-45(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
    Tissue: Small intestine.
  2. "Generation and annotation of the DNA sequences of human chromosomes 2 and 4."
    Hillier L.W., Graves T.A., Fulton R.S., Fulton L.A., Pepin K.H., Minx P., Wagner-McPherson C., Layman D., Wylie K., Sekhon M., Becker M.C., Fewell G.A., Delehaunty K.D., Miner T.L., Nash W.E., Kremitzki C., Oddy L., Du H.
    , Sun H., Bradshaw-Cordum H., Ali J., Carter J., Cordes M., Harris A., Isak A., van Brunt A., Nguyen C., Du F., Courtney L., Kalicki J., Ozersky P., Abbott S., Armstrong J., Belter E.A., Caruso L., Cedroni M., Cotton M., Davidson T., Desai A., Elliott G., Erb T., Fronick C., Gaige T., Haakenson W., Haglund K., Holmes A., Harkins R., Kim K., Kruchowski S.S., Strong C.M., Grewal N., Goyea E., Hou S., Levy A., Martinka S., Mead K., McLellan M.D., Meyer R., Randall-Maher J., Tomlinson C., Dauphin-Kohlberg S., Kozlowicz-Reilly A., Shah N., Swearengen-Shahid S., Snider J., Strong J.T., Thompson J., Yoakum M., Leonard S., Pearman C., Trani L., Radionenko M., Waligorski J.E., Wang C., Rock S.M., Tin-Wollam A.-M., Maupin R., Latreille P., Wendl M.C., Yang S.-P., Pohl C., Wallis J.W., Spieth J., Bieri T.A., Berkowicz N., Nelson J.O., Osborne J., Ding L., Meyer R., Sabo A., Shotland Y., Sinha P., Wohldmann P.E., Cook L.L., Hickenbotham M.T., Eldred J., Williams D., Jones T.A., She X., Ciccarelli F.D., Izaurralde E., Taylor J., Schmutz J., Myers R.M., Cox D.R., Huang X., McPherson J.D., Mardis E.R., Clifton S.W., Warren W.C., Chinwalla A.T., Eddy S.R., Marra M.A., Ovcharenko I., Furey T.S., Miller W., Eichler E.E., Bork P., Suyama M., Torrents D., Waterston R.H., Wilson R.K.
    Nature 434:724-731(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
  3. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORMS 1 AND 2).
    Tissue: Brain and Lung.
  4. "The 2p21 deletion syndrome: characterization of the transcription content."
    Parvari R., Gonen Y., Alshafee I., Buriakovsky S., Regev K., Hershkovitz E.
    Genomics 86:195-211(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: TISSUE SPECIFICITY.
  5. "Calmodulin methyltransferase is an evolutionarily conserved enzyme that trimethylates Lys-115 in calmodulin."
    Magnani R., Dirk L.M., Trievel R.C., Houtz R.L.
    Nat. Commun. 1:43-43(2010) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, CATALYTIC ACTIVITY.
  6. Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
  7. "Human calmodulin methyltransferase: expression, activity on calmodulin, and Hsp90 dependence."
    Magen S., Magnani R., Haziza S., Hershkovitz E., Houtz R., Cambi F., Parvari R.
    PLoS ONE 7:E52425-E52425(2012) [PubMed] [Europe PMC] [Abstract]
    Cited for: SUBCELLULAR LOCATION, INTERACTION WITH HSP90.

Entry informationi

Entry nameiCMKMT_HUMAN
AccessioniPrimary (citable) accession number: Q7Z624
Secondary accession number(s): Q4ZG15
, Q53SS6, Q8N6P5, Q9H5G8
Entry historyi
Integrated into UniProtKB/Swiss-Prot: September 11, 2007
Last sequence update: September 11, 2007
Last modified: June 8, 2016
This is version 93 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. Human chromosome 2
    Human chromosome 2: entries, gene names and cross-references to MIM
  2. MIM cross-references
    Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
  3. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  4. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.