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Q7Z5W3

- BN3D2_HUMAN

UniProt

Q7Z5W3 - BN3D2_HUMAN

Protein

Pre-miRNA 5'-monophosphate methyltransferase

Gene

BCDIN3D

Organism
Homo sapiens (Human)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 80 (01 Oct 2014)
      Sequence version 1 (01 Oct 2003)
      Previous versions | rss
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    Functioni

    O-methyltransferase that specifically dimethylates the 5' monophosphate of pre-miRNAs, acting as a negative regulator of miRNA processing. The 5' monophosphate of pre-miRNAs is recognized by DICER1 and is required for pre-miRNAs processing: methylation at this position reduces the processing of pre-miRNAs by DICER1. Able to mediate methylation of pre-miR-145, as well as other pre-miRNAs.1 Publication

    GO - Molecular functioni

    1. O-methyltransferase activity Source: UniProtKB
    2. RNA methyltransferase activity Source: UniProtKB

    GO - Biological processi

    1. miRNA metabolic process Source: UniProtKB
    2. negative regulation of pre-miRNA processing Source: UniProtKB
    3. RNA methylation Source: UniProtKB

    Keywords - Molecular functioni

    Methyltransferase, Transferase

    Keywords - Ligandi

    S-adenosyl-L-methionine

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Pre-miRNA 5'-monophosphate methyltransferase (EC:2.1.1.-)
    Alternative name(s):
    BCDIN3 domain-containing protein
    Gene namesi
    Name:BCDIN3D
    OrganismiHomo sapiens (Human)
    Taxonomic identifieri9606 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
    ProteomesiUP000005640: Chromosome 12

    Organism-specific databases

    HGNCiHGNC:27050. BCDIN3D.

    Subcellular locationi

    Cytoplasm 1 Publication

    GO - Cellular componenti

    1. cytoplasm Source: UniProtKB
    2. nucleus Source: HPA

    Keywords - Cellular componenti

    Cytoplasm

    Pathology & Biotechi

    Mutagenesis

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Mutagenesisi72 – 743DVG → AVA: Abolishes methyltransferase activity. 1 Publication

    Organism-specific databases

    PharmGKBiPA162377410.

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 292292Pre-miRNA 5'-monophosphate methyltransferasePRO_0000289265Add
    BLAST

    Proteomic databases

    MaxQBiQ7Z5W3.
    PaxDbiQ7Z5W3.
    PRIDEiQ7Z5W3.

    PTM databases

    PhosphoSiteiQ7Z5W3.

    Expressioni

    Gene expression databases

    BgeeiQ7Z5W3.
    CleanExiHS_BCDIN3D.
    GenevestigatoriQ7Z5W3.

    Organism-specific databases

    HPAiHPA039911.

    Interactioni

    Subunit structurei

    Interacts with DICER1; the interaction may be mediated by RNA.1 Publication

    Protein-protein interaction databases

    BioGridi126839. 3 interactions.
    STRINGi9606.ENSP00000335201.

    Structurei

    3D structure databases

    ProteinModelPortaliQ7Z5W3.
    SMRiQ7Z5W3. Positions 46-203.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Domaini53 – 274222Bin3-type SAMPROSITE-ProRule annotationAdd
    BLAST

    Sequence similaritiesi

    Belongs to the methyltransferase superfamily.Curated
    Contains 1 Bin3-type SAM domain.PROSITE-ProRule annotation

    Phylogenomic databases

    eggNOGiNOG255867.
    HOGENOMiHOG000013152.
    HOVERGENiHBG057674.
    InParanoidiQ7Z5W3.
    OMAiCYRSAAR.
    OrthoDBiEOG715Q5R.
    PhylomeDBiQ7Z5W3.
    TreeFamiTF324061.

    Family and domain databases

    Gene3Di3.40.50.150. 1 hit.
    InterProiIPR010675. Bin3.
    IPR024160. BIN3_SAM-bd_dom.
    IPR029063. SAM-dependent_MTases-like.
    [Graphical view]
    PfamiPF06859. Bin3. 1 hit.
    [Graphical view]
    SUPFAMiSSF53335. SSF53335. 1 hit.
    PROSITEiPS51515. BIN3_SAM. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Q7Z5W3-1 [UniParc]FASTAAdd to Basket

    « Hide

    MAVPTELDGG SVKETAAEEE SRVLAPGAAP FGNFPHYSRF HPPEQRLRLL    50
    PPELLRQLFP ESPENGPILG LDVGCNSGDL SVALYKHFLS LPDGETCSDA 100
    SREFRLLCCD IDPVLVKRAE KECPFPDALT FITLDFMNQR TRKVLLSSFL 150
    SQFGRSVFDI GFCMSITMWI HLNHGDHGLW EFLAHLSSLC HYLLVEPQPW 200
    KCYRAAARRL RKLGLHDFDH FHSLAIRGDM PNQIVQILTQ DHGMELICCF 250
    GNTSWDRSLL LFRAKQTIET HPIPESLIEK GKEKNRLSFQ KQ 292
    Length:292
    Mass (Da):33,200
    Last modified:October 1, 2003 - v1
    Checksum:i361EF0BBAAC0CCAF
    GO

    Natural variant

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Natural varianti288 – 2881S → R.
    Corresponds to variant rs11169172 [ dbSNP | Ensembl ].
    VAR_032614

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AK292194 mRNA. Translation: BAF84883.1.
    CH471111 Genomic DNA. Translation: EAW58101.1.
    BC053560 mRNA. Translation: AAH53560.1.
    CCDSiCCDS8790.1.
    RefSeqiNP_859059.1. NM_181708.2.
    UniGeneiHs.142736.

    Genome annotation databases

    EnsembliENST00000333924; ENSP00000335201; ENSG00000186666.
    GeneIDi144233.
    KEGGihsa:144233.
    UCSCiuc001rvh.3. human.

    Polymorphism databases

    DMDMi74738762.

    Keywords - Coding sequence diversityi

    Polymorphism

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AK292194 mRNA. Translation: BAF84883.1 .
    CH471111 Genomic DNA. Translation: EAW58101.1 .
    BC053560 mRNA. Translation: AAH53560.1 .
    CCDSi CCDS8790.1.
    RefSeqi NP_859059.1. NM_181708.2.
    UniGenei Hs.142736.

    3D structure databases

    ProteinModelPortali Q7Z5W3.
    SMRi Q7Z5W3. Positions 46-203.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    BioGridi 126839. 3 interactions.
    STRINGi 9606.ENSP00000335201.

    PTM databases

    PhosphoSitei Q7Z5W3.

    Polymorphism databases

    DMDMi 74738762.

    Proteomic databases

    MaxQBi Q7Z5W3.
    PaxDbi Q7Z5W3.
    PRIDEi Q7Z5W3.

    Protocols and materials databases

    DNASUi 144233.
    Structural Biology Knowledgebase Search...

    Genome annotation databases

    Ensembli ENST00000333924 ; ENSP00000335201 ; ENSG00000186666 .
    GeneIDi 144233.
    KEGGi hsa:144233.
    UCSCi uc001rvh.3. human.

    Organism-specific databases

    CTDi 144233.
    GeneCardsi GC12M050231.
    HGNCi HGNC:27050. BCDIN3D.
    HPAi HPA039911.
    neXtProti NX_Q7Z5W3.
    PharmGKBi PA162377410.
    GenAtlasi Search...

    Phylogenomic databases

    eggNOGi NOG255867.
    HOGENOMi HOG000013152.
    HOVERGENi HBG057674.
    InParanoidi Q7Z5W3.
    OMAi CYRSAAR.
    OrthoDBi EOG715Q5R.
    PhylomeDBi Q7Z5W3.
    TreeFami TF324061.

    Miscellaneous databases

    GenomeRNAii 144233.
    NextBioi 84869.
    PROi Q7Z5W3.

    Gene expression databases

    Bgeei Q7Z5W3.
    CleanExi HS_BCDIN3D.
    Genevestigatori Q7Z5W3.

    Family and domain databases

    Gene3Di 3.40.50.150. 1 hit.
    InterProi IPR010675. Bin3.
    IPR024160. BIN3_SAM-bd_dom.
    IPR029063. SAM-dependent_MTases-like.
    [Graphical view ]
    Pfami PF06859. Bin3. 1 hit.
    [Graphical view ]
    SUPFAMi SSF53335. SSF53335. 1 hit.
    PROSITEi PS51515. BIN3_SAM. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Complete sequencing and characterization of 21,243 full-length human cDNAs."
      Ota T., Suzuki Y., Nishikawa T., Otsuki T., Sugiyama T., Irie R., Wakamatsu A., Hayashi K., Sato H., Nagai K., Kimura K., Makita H., Sekine M., Obayashi M., Nishi T., Shibahara T., Tanaka T., Ishii S.
      , Yamamoto J., Saito K., Kawai Y., Isono Y., Nakamura Y., Nagahari K., Murakami K., Yasuda T., Iwayanagi T., Wagatsuma M., Shiratori A., Sudo H., Hosoiri T., Kaku Y., Kodaira H., Kondo H., Sugawara M., Takahashi M., Kanda K., Yokoi T., Furuya T., Kikkawa E., Omura Y., Abe K., Kamihara K., Katsuta N., Sato K., Tanikawa M., Yamazaki M., Ninomiya K., Ishibashi T., Yamashita H., Murakawa K., Fujimori K., Tanai H., Kimata M., Watanabe M., Hiraoka S., Chiba Y., Ishida S., Ono Y., Takiguchi S., Watanabe S., Yosida M., Hotuta T., Kusano J., Kanehori K., Takahashi-Fujii A., Hara H., Tanase T.-O., Nomura Y., Togiya S., Komai F., Hara R., Takeuchi K., Arita M., Imose N., Musashino K., Yuuki H., Oshima A., Sasaki N., Aotsuka S., Yoshikawa Y., Matsunawa H., Ichihara T., Shiohata N., Sano S., Moriya S., Momiyama H., Satoh N., Takami S., Terashima Y., Suzuki O., Nakagawa S., Senoh A., Mizoguchi H., Goto Y., Shimizu F., Wakebe H., Hishigaki H., Watanabe T., Sugiyama A., Takemoto M., Kawakami B., Yamazaki M., Watanabe K., Kumagai A., Itakura S., Fukuzumi Y., Fujimori Y., Komiyama M., Tashiro H., Tanigami A., Fujiwara T., Ono T., Yamada K., Fujii Y., Ozaki K., Hirao M., Ohmori Y., Kawabata A., Hikiji T., Kobatake N., Inagaki H., Ikema Y., Okamoto S., Okitani R., Kawakami T., Noguchi S., Itoh T., Shigeta K., Senba T., Matsumura K., Nakajima Y., Mizuno T., Morinaga M., Sasaki M., Togashi T., Oyama M., Hata H., Watanabe M., Komatsu T., Mizushima-Sugano J., Satoh T., Shirai Y., Takahashi Y., Nakagawa K., Okumura K., Nagase T., Nomura N., Kikuchi H., Masuho Y., Yamashita R., Nakai K., Yada T., Nakamura Y., Ohara O., Isogai T., Sugano S.
      Nat. Genet. 36:40-45(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
      Tissue: Cervix.
    2. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    3. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
      The MGC Project Team
      Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
      Tissue: Skin.
    4. "Human RNA methyltransferase BCDIN3D regulates microRNA processing."
      Xhemalce B., Robson S.C., Kouzarides T.
      Cell 151:278-288(2012) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION, CATALYTIC ACTIVITY, INTERACTION WITH DICER1, MUTAGENESIS OF 72-ASP--GLU-74, SUBCELLULAR LOCATION.

    Entry informationi

    Entry nameiBN3D2_HUMAN
    AccessioniPrimary (citable) accession number: Q7Z5W3
    Secondary accession number(s): A8K829
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: May 29, 2007
    Last sequence update: October 1, 2003
    Last modified: October 1, 2014
    This is version 80 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program
    DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. Human chromosome 12
      Human chromosome 12: entries, gene names and cross-references to MIM
    2. Human entries with polymorphisms or disease mutations
      List of human entries with polymorphisms or disease mutations
    3. Human polymorphisms and disease mutations
      Index of human polymorphisms and disease mutations
    4. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3