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Q7Z5W3 (BN3D2_HUMAN) Reviewed, UniProtKB/Swiss-Prot

Last modified April 16, 2014. Version 76. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (4) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Pre-miRNA 5'-monophosphate methyltransferase

EC=2.1.1.-
Alternative name(s):
BCDIN3 domain-containing protein
Gene names
Name:BCDIN3D
OrganismHomo sapiens (Human) [Reference proteome]
Taxonomic identifier9606 [NCBI]
Taxonomic lineageEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo

Protein attributes

Sequence length292 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

O-methyltransferase that specifically dimethylates the 5' monophosphate of pre-miRNAs, acting as a negative regulator of miRNA processing. The 5' monophosphate of pre-miRNAs is recognized by DICER1 and is required for pre-miRNAs processing: methylation at this position reduces the processing of pre-miRNAs by DICER1. Able to mediate methylation of pre-miR-145, as well as other pre-miRNAs. Ref.4

Subunit structure

Interacts with DICER1; the interaction may be mediated by RNA. Ref.4

Subcellular location

Cytoplasm Ref.4.

Sequence similarities

Belongs to the methyltransferase superfamily.

Contains 1 Bin3-type SAM domain.

Ontologies

Keywords
   Cellular componentCytoplasm
   Coding sequence diversityPolymorphism
   LigandS-adenosyl-L-methionine
   Molecular functionMethyltransferase
Transferase
   Technical termComplete proteome
Reference proteome
Gene Ontology (GO)
   Biological_processRNA methylation

Inferred from direct assay Ref.4. Source: UniProtKB

miRNA metabolic process

Inferred from direct assay Ref.4. Source: UniProtKB

negative regulation of pre-miRNA processing

Inferred from direct assay Ref.4. Source: UniProtKB

   Cellular_componentcytoplasm

Inferred from direct assay Ref.4. Source: UniProtKB

nucleus

Inferred from direct assay. Source: HPA

   Molecular_functionO-methyltransferase activity

Inferred from direct assay Ref.4. Source: UniProtKB

RNA methyltransferase activity

Inferred from direct assay Ref.4. Source: UniProtKB

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 292292Pre-miRNA 5'-monophosphate methyltransferase
PRO_0000289265

Regions

Domain53 – 274222Bin3-type SAM

Natural variations

Natural variant2881S → R.
Corresponds to variant rs11169172 [ dbSNP | Ensembl ].
VAR_032614

Experimental info

Mutagenesis72 – 743DVG → AVA: Abolishes methyltransferase activity. Ref.4

Sequences

Sequence LengthMass (Da)Tools
Q7Z5W3 [UniParc].

Last modified October 1, 2003. Version 1.
Checksum: 361EF0BBAAC0CCAF

FASTA29233,200
        10         20         30         40         50         60 
MAVPTELDGG SVKETAAEEE SRVLAPGAAP FGNFPHYSRF HPPEQRLRLL PPELLRQLFP 

        70         80         90        100        110        120 
ESPENGPILG LDVGCNSGDL SVALYKHFLS LPDGETCSDA SREFRLLCCD IDPVLVKRAE 

       130        140        150        160        170        180 
KECPFPDALT FITLDFMNQR TRKVLLSSFL SQFGRSVFDI GFCMSITMWI HLNHGDHGLW 

       190        200        210        220        230        240 
EFLAHLSSLC HYLLVEPQPW KCYRAAARRL RKLGLHDFDH FHSLAIRGDM PNQIVQILTQ 

       250        260        270        280        290 
DHGMELICCF GNTSWDRSLL LFRAKQTIET HPIPESLIEK GKEKNRLSFQ KQ 

« Hide

References

« Hide 'large scale' references
[1]"Complete sequencing and characterization of 21,243 full-length human cDNAs."
Ota T., Suzuki Y., Nishikawa T., Otsuki T., Sugiyama T., Irie R., Wakamatsu A., Hayashi K., Sato H., Nagai K., Kimura K., Makita H., Sekine M., Obayashi M., Nishi T., Shibahara T., Tanaka T., Ishii S. expand/collapse author list , Yamamoto J., Saito K., Kawai Y., Isono Y., Nakamura Y., Nagahari K., Murakami K., Yasuda T., Iwayanagi T., Wagatsuma M., Shiratori A., Sudo H., Hosoiri T., Kaku Y., Kodaira H., Kondo H., Sugawara M., Takahashi M., Kanda K., Yokoi T., Furuya T., Kikkawa E., Omura Y., Abe K., Kamihara K., Katsuta N., Sato K., Tanikawa M., Yamazaki M., Ninomiya K., Ishibashi T., Yamashita H., Murakawa K., Fujimori K., Tanai H., Kimata M., Watanabe M., Hiraoka S., Chiba Y., Ishida S., Ono Y., Takiguchi S., Watanabe S., Yosida M., Hotuta T., Kusano J., Kanehori K., Takahashi-Fujii A., Hara H., Tanase T.-O., Nomura Y., Togiya S., Komai F., Hara R., Takeuchi K., Arita M., Imose N., Musashino K., Yuuki H., Oshima A., Sasaki N., Aotsuka S., Yoshikawa Y., Matsunawa H., Ichihara T., Shiohata N., Sano S., Moriya S., Momiyama H., Satoh N., Takami S., Terashima Y., Suzuki O., Nakagawa S., Senoh A., Mizoguchi H., Goto Y., Shimizu F., Wakebe H., Hishigaki H., Watanabe T., Sugiyama A., Takemoto M., Kawakami B., Yamazaki M., Watanabe K., Kumagai A., Itakura S., Fukuzumi Y., Fujimori Y., Komiyama M., Tashiro H., Tanigami A., Fujiwara T., Ono T., Yamada K., Fujii Y., Ozaki K., Hirao M., Ohmori Y., Kawabata A., Hikiji T., Kobatake N., Inagaki H., Ikema Y., Okamoto S., Okitani R., Kawakami T., Noguchi S., Itoh T., Shigeta K., Senba T., Matsumura K., Nakajima Y., Mizuno T., Morinaga M., Sasaki M., Togashi T., Oyama M., Hata H., Watanabe M., Komatsu T., Mizushima-Sugano J., Satoh T., Shirai Y., Takahashi Y., Nakagawa K., Okumura K., Nagase T., Nomura N., Kikuchi H., Masuho Y., Yamashita R., Nakai K., Yada T., Nakamura Y., Ohara O., Isogai T., Sugano S.
Nat. Genet. 36:40-45(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
Tissue: Cervix.
[2]Mural R.J., Istrail S., Sutton G.G., Florea L., Halpern A.L., Mobarry C.M., Lippert R., Walenz B., Shatkay H., Dew I., Miller J.R., Flanigan M.J., Edwards N.J., Bolanos R., Fasulo D., Halldorsson B.V., Hannenhalli S., Turner R. expand/collapse author list , Yooseph S., Lu F., Nusskern D.R., Shue B.C., Zheng X.H., Zhong F., Delcher A.L., Huson D.H., Kravitz S.A., Mouchard L., Reinert K., Remington K.A., Clark A.G., Waterman M.S., Eichler E.E., Adams M.D., Hunkapiller M.W., Myers E.W., Venter J.C.
Submitted (JUL-2005) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[3]"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
The MGC Project Team
Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
Tissue: Skin.
[4]"Human RNA methyltransferase BCDIN3D regulates microRNA processing."
Xhemalce B., Robson S.C., Kouzarides T.
Cell 151:278-288(2012) [PubMed] [Europe PMC] [Abstract]
Cited for: FUNCTION, CATALYTIC ACTIVITY, INTERACTION WITH DICER1, MUTAGENESIS OF 72-ASP--GLU-74, SUBCELLULAR LOCATION.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AK292194 mRNA. Translation: BAF84883.1.
CH471111 Genomic DNA. Translation: EAW58101.1.
BC053560 mRNA. Translation: AAH53560.1.
RefSeqNP_859059.1. NM_181708.2.
UniGeneHs.142736.

3D structure databases

ProteinModelPortalQ7Z5W3.
SMRQ7Z5W3. Positions 45-265.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

BioGrid126839. 3 interactions.
STRING9606.ENSP00000335201.

PTM databases

PhosphoSiteQ7Z5W3.

Polymorphism databases

DMDM74738762.

Proteomic databases

PaxDbQ7Z5W3.
PRIDEQ7Z5W3.

Protocols and materials databases

DNASU144233.
StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblENST00000333924; ENSP00000335201; ENSG00000186666.
GeneID144233.
KEGGhsa:144233.
UCSCuc001rvh.3. human.

Organism-specific databases

CTD144233.
GeneCardsGC12M050231.
HGNCHGNC:27050. BCDIN3D.
HPAHPA039911.
neXtProtNX_Q7Z5W3.
PharmGKBPA162377410.
GenAtlasSearch...

Phylogenomic databases

eggNOGNOG255867.
HOGENOMHOG000013152.
HOVERGENHBG057674.
InParanoidQ7Z5W3.
OMACYRSAAR.
OrthoDBEOG715Q5R.
PhylomeDBQ7Z5W3.
TreeFamTF324061.

Gene expression databases

BgeeQ7Z5W3.
CleanExHS_BCDIN3D.
GenevestigatorQ7Z5W3.

Family and domain databases

InterProIPR010675. Bin3.
IPR024160. BIN3_SAM-bd_dom.
[Graphical view]
PfamPF06859. Bin3. 1 hit.
[Graphical view]
PROSITEPS51515. BIN3_SAM. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

GenomeRNAi144233.
NextBio84869.
PROQ7Z5W3.

Entry information

Entry nameBN3D2_HUMAN
AccessionPrimary (citable) accession number: Q7Z5W3
Secondary accession number(s): A8K829
Entry history
Integrated into UniProtKB/Swiss-Prot: May 29, 2007
Last sequence update: October 1, 2003
Last modified: April 16, 2014
This is version 76 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Relevant documents

SIMILARITY comments

Index of protein domains and families

Human polymorphisms and disease mutations

Index of human polymorphisms and disease mutations

Human entries with polymorphisms or disease mutations

List of human entries with polymorphisms or disease mutations

Human chromosome 12

Human chromosome 12: entries, gene names and cross-references to MIM