Q7Z5W3 (BN3D2_HUMAN) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Pre-miRNA 5'-monophosphate methyltransferase
EC=2.1.1.-

Alternative name(s):
BCDIN3 domain-containing protein

Gene names

Name:

BCDIN3D

Organism

Homo sapiens (Human) [Reference proteome]

Taxonomic identifier

9606 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Deuterostomia › Chordata › Craniata › Vertebrata › Gnathostomata › Teleostomi › Euteleostomi › Sarcopterygii › Tetrapoda › Amniota › Mammalia › Theria › Eutheria › Euarchontoglires › Primates › Haplorrhini › Simiiformes › Catarrhini › Hominoidea › Hominidae › Homininae › Homo

Protein attributes

Sequence length	292 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	O-methyltransferase that specifically dimethylates the 5' monophosphate of pre-miRNAs, acting as a negative regulator of miRNA processing. The 5' monophosphate of pre-miRNAs is recognized by DICER1 and is required for pre-miRNAs processing: methylation at this position reduces the processing of pre-miRNAs by DICER1. Able to mediate methylation of pre-miR-145, as well as other pre-miRNAs. Ref.4
Subunit structure	Interacts with DICER1; the interaction may be mediated by RNA. Ref.4
Subcellular location	Cytoplasm Ref.4.
Sequence similarities	Belongs to the methyltransferase superfamily. Contains 1 Bin3-type SAM domain.

Ontologies

Keywords
Cellular component	Cytoplasm
Coding sequence diversity	Polymorphism
Ligand	S-adenosyl-L-methionine
Molecular function	Methyltransferase Transferase
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	miRNA metabolic process Inferred from direct assay Ref.4. Source: UniProtKB negative regulation of pre-miRNA processing Inferred from direct assay Ref.4. Source: UniProtKB
Cellular_component	cytoplasm Inferred from direct assay Ref.4. Source: UniProtKB
Molecular_function	O-methyltransferase activity Inferred from direct assay Ref.4. Source: UniProtKB RNA methyltransferase activity Inferred from direct assay Ref.4. Source: UniProtKB
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 292	292	Pre-miRNA 5'-monophosphate methyltransferase		PRO_0000289265
Regions
	Domain	53 – 274	222	Bin3-type SAM
Natural variations
	Natural variant	288	1	S → R. Corresponds to variant rs11169172 [ dbSNP | Ensembl ].		VAR_032614
Experimental info
	Mutagenesis	72 – 74	3	DVG → AVA: Abolishes methyltransferase activity. Ref.4

Sequences

	Sequence		Length	Mass (Da)
	Q7Z5W3 [UniParc]. Last modified October 1, 2003. Version 1. Checksum: 361EF0BBAAC0CCAF	FASTA	292	33,200
	10 20 30 40 50 60 MAVPTELDGG SVKETAAEEE SRVLAPGAAP FGNFPHYSRF HPPEQRLRLL PPELLRQLFP 70 80 90 100 110 120 ESPENGPILG LDVGCNSGDL SVALYKHFLS LPDGETCSDA SREFRLLCCD IDPVLVKRAE 130 140 150 160 170 180 KECPFPDALT FITLDFMNQR TRKVLLSSFL SQFGRSVFDI GFCMSITMWI HLNHGDHGLW 190 200 210 220 230 240 EFLAHLSSLC HYLLVEPQPW KCYRAAARRL RKLGLHDFDH FHSLAIRGDM PNQIVQILTQ 250 260 270 280 290 DHGMELICCF GNTSWDRSLL LFRAKQTIET HPIPESLIEK GKEKNRLSFQ KQ

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Complete sequencing and characterization of 21,243 full-length human cDNAs." Ota T., Suzuki Y., Nishikawa T., Otsuki T., Sugiyama T., Irie R., Wakamatsu A., Hayashi K., Sato H., Nagai K., Kimura K., Makita H., Sekine M., Obayashi M., Nishi T., Shibahara T., Tanaka T., Ishii S. , Yamamoto J., Saito K., Kawai Y., Isono Y., Nakamura Y., Nagahari K., Murakami K., Yasuda T., Iwayanagi T., Wagatsuma M., Shiratori A., Sudo H., Hosoiri T., Kaku Y., Kodaira H., Kondo H., Sugawara M., Takahashi M., Kanda K., Yokoi T., Furuya T., Kikkawa E., Omura Y., Abe K., Kamihara K., Katsuta N., Sato K., Tanikawa M., Yamazaki M., Ninomiya K., Ishibashi T., Yamashita H., Murakawa K., Fujimori K., Tanai H., Kimata M., Watanabe M., Hiraoka S., Chiba Y., Ishida S., Ono Y., Takiguchi S., Watanabe S., Yosida M., Hotuta T., Kusano J., Kanehori K., Takahashi-Fujii A., Hara H., Tanase T.-O., Nomura Y., Togiya S., Komai F., Hara R., Takeuchi K., Arita M., Imose N., Musashino K., Yuuki H., Oshima A., Sasaki N., Aotsuka S., Yoshikawa Y., Matsunawa H., Ichihara T., Shiohata N., Sano S., Moriya S., Momiyama H., Satoh N., Takami S., Terashima Y., Suzuki O., Nakagawa S., Senoh A., Mizoguchi H., Goto Y., Shimizu F., Wakebe H., Hishigaki H., Watanabe T., Sugiyama A., Takemoto M., Kawakami B., Yamazaki M., Watanabe K., Kumagai A., Itakura S., Fukuzumi Y., Fujimori Y., Komiyama M., Tashiro H., Tanigami A., Fujiwara T., Ono T., Yamada K., Fujii Y., Ozaki K., Hirao M., Ohmori Y., Kawabata A., Hikiji T., Kobatake N., Inagaki H., Ikema Y., Okamoto S., Okitani R., Kawakami T., Noguchi S., Itoh T., Shigeta K., Senba T., Matsumura K., Nakajima Y., Mizuno T., Morinaga M., Sasaki M., Togashi T., Oyama M., Hata H., Watanabe M., Komatsu T., Mizushima-Sugano J., Satoh T., Shirai Y., Takahashi Y., Nakagawa K., Okumura K., Nagase T., Nomura N., Kikuchi H., Masuho Y., Yamashita R., Nakai K., Yada T., Nakamura Y., Ohara O., Isogai T., Sugano S. Nat. Genet. 36:40-45(2004) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Tissue: Cervix.
[2]	Mural R.J., Istrail S., Sutton G.G., Florea L., Halpern A.L., Mobarry C.M., Lippert R., Walenz B., Shatkay H., Dew I., Miller J.R., Flanigan M.J., Edwards N.J., Bolanos R., Fasulo D., Halldorsson B.V., Hannenhalli S., Turner R. , Yooseph S., Lu F., Nusskern D.R., Shue B.C., Zheng X.H., Zhong F., Delcher A.L., Huson D.H., Kravitz S.A., Mouchard L., Reinert K., Remington K.A., Clark A.G., Waterman M.S., Eichler E.E., Adams M.D., Hunkapiller M.W., Myers E.W., Venter J.C. Submitted (JUL-2005) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[3]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Tissue: Skin.
[4]	"Human RNA methyltransferase BCDIN3D regulates microRNA processing." Xhemalce B., Robson S.C., Kouzarides T. Cell 151:278-288(2012) [PubMed] [Europe PMC] [Abstract] Cited for: FUNCTION, CATALYTIC ACTIVITY, INTERACTION WITH DICER1, MUTAGENESIS OF 72-ASP--GLU-74, SUBCELLULAR LOCATION.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AK292194 mRNA. Translation: BAF84883.1. CH471111 Genomic DNA. Translation: EAW58101.1. BC053560 mRNA. Translation: AAH53560.1.
IPI	IPI00376228.
RefSeq	NP_859059.1. NM_181708.2.
UniGene	Hs.142736.
3D structure databases
ProteinModelPortal	Q7Z5W3.
ModBase	Search...
Protein-protein interaction databases
STRING	9606.ENSP00000335201.
PTM databases
PhosphoSite	Q7Z5W3.
Polymorphism databases
DMDM	74738762.
Proteomic databases
PaxDb	Q7Z5W3.
PRIDE	Q7Z5W3.
Protocols and materials databases
DNASU	144233.
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
Ensembl	ENST00000333924; ENSP00000335201; ENSG00000186666.
GeneID	144233.
KEGG	hsa:144233.
UCSC	uc001rvh.3. human.
Organism-specific databases
CTD	144233.
GeneCards	GC12M050231.
HGNC	HGNC:27050. BCDIN3D.
HPA	HPA039911.
neXtProt	NX_Q7Z5W3.
PharmGKB	PA162377410.
GenAtlas	Search...
Phylogenomic databases
eggNOG	NOG255867.
HOGENOM	HOG000013152.
HOVERGEN	HBG057674.
InParanoid	Q7Z5W3.
OMA	FDHFHSL.
OrthoDB	EOG4RBQK6.
PhylomeDB	Q7Z5W3.
Gene expression databases
ArrayExpress	Q7Z5W3.
Bgee	Q7Z5W3.
CleanEx	HS_BCDIN3D.
Genevestigator	Q7Z5W3.
Family and domain databases
InterPro	IPR010675. Bin3. IPR024160. BIN3_SAM-bd_dom. [Graphical view]
Pfam	PF06859. Bin3. 1 hit. [Graphical view]
PROSITE	PS51515. BIN3_SAM. 1 hit. [Graphical view]
ProtoNet	Search...
Other
GenomeRNAi	144233.
NextBio	84869.

Entry information

Entry name

BN3D2_HUMAN

Accession

Primary (citable) accession number: Q7Z5W3
Secondary accession number(s): A8K829

Entry history

Integrated into UniProtKB/Swiss-Prot:	May 29, 2007
Last sequence update:	October 1, 2003
Last modified:	May 1, 2013
This is version 68 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program

Disclaimer

Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.