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Protein

Amyloid beta A4 precursor protein-binding family B member 1-interacting protein

Gene

APBB1IP

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Appears to function in the signal transduction from Ras activation to actin cytoskeletal remodeling. Suppresses insulin-induced promoter activities through AP1 and SRE. Mediates Rap1-induced adhesion.2 Publications

GO - Biological processi

Complete GO annotation...

Enzyme and pathway databases

ReactomeiR-HSA-354192. Integrin alphaIIb beta3 signaling.
R-HSA-354194. GRB2:SOS provides linkage to MAPK signaling for Integrins.
R-HSA-372708. p130Cas linkage to MAPK signaling for integrins.
R-HSA-5674135. MAP2K and MAPK activation.
R-HSA-6802946. Signaling by moderate kinase activity BRAF mutants.
R-HSA-6802948. Signaling by high-kinase activity BRAF mutants.
R-HSA-6802949. Signaling by RAS mutants.
R-HSA-6802952. Signaling by BRAF and RAF fusions.
R-HSA-6802955. Paradoxical activation of RAF signaling by kinase inactive BRAF.

Names & Taxonomyi

Protein namesi
Recommended name:
Amyloid beta A4 precursor protein-binding family B member 1-interacting protein
Alternative name(s):
APBB1-interacting protein 1
Proline-rich EVH1 ligand 1
Short name:
PREL-1
Proline-rich protein 73
Rap1-GTP-interacting adapter molecule
Short name:
RIAM
Retinoic acid-responsive proline-rich protein 1
Short name:
RARP-1
Gene namesi
Name:APBB1IP
Synonyms:PREL1, RARP1, RIAM
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
Proteomesi
  • UP000005640 Componenti: Chromosome 10

Organism-specific databases

HGNCiHGNC:17379. APBB1IP.

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cell junction, Cell membrane, Cell projection, Cytoplasm, Cytoskeleton, Membrane

Pathology & Biotechi

Organism-specific databases

DisGeNETi54518.
OpenTargetsiENSG00000077420.
PharmGKBiPA134933955.

Polymorphism and mutation databases

BioMutaiAPBB1IP.
DMDMi74750143.

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001813471 – 666Amyloid beta A4 precursor protein-binding family B member 1-interacting proteinAdd BLAST666

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei55PhosphoserineCombined sources1
Modified residuei526PhosphoserineCombined sources1
Modified residuei528PhosphothreonineBy similarity1
Modified residuei531PhosphoserineBy similarity1

Keywords - PTMi

Phosphoprotein

Proteomic databases

EPDiQ7Z5R6.
MaxQBiQ7Z5R6.
PaxDbiQ7Z5R6.
PeptideAtlasiQ7Z5R6.
PRIDEiQ7Z5R6.

PTM databases

iPTMnetiQ7Z5R6.
PhosphoSitePlusiQ7Z5R6.

Expressioni

Tissue specificityi

Widely expressed with high expression in thymus, spleen, lymph node, bone marrow and peripheral leukocytes.2 Publications

Inductioni

By all-trans-retinoic acid (ATRA).

Gene expression databases

BgeeiENSG00000077420.
CleanExiHS_APBB1IP.
GenevisibleiQ7Z5R6. HS.

Organism-specific databases

HPAiHPA017009.
HPA063903.

Interactioni

Subunit structurei

Interacts, through the N-terminal Pro-rich region, with the WW domain of APBB1. Interacts with RAP1A, PFN1, TLN1, VASP, VCL and ENAH.2 Publications

Binary interactionsi

WithEntry#Exp.IntActNotes
ZDHHC17Q8IUH52EBI-2818084,EBI-524753

Protein-protein interaction databases

BioGridi120012. 10 interactors.
IntActiQ7Z5R6. 11 interactors.
STRINGi9606.ENSP00000365411.

Structurei

Secondary structure

1666
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Helixi8 – 25Combined sources18
Turni26 – 31Combined sources6

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
2MWNNMR-A7-30[»]
3ZDLX-ray2.30B1-31[»]
ProteinModelPortaliQ7Z5R6.
SMRiQ7Z5R6.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini176 – 263Ras-associatingPROSITE-ProRule annotationAdd BLAST88
Domaini310 – 419PHPROSITE-ProRule annotationAdd BLAST110

Compositional bias

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Compositional biasi129 – 148Pro-richAdd BLAST20
Compositional biasi152 – 155Poly-Glu4
Compositional biasi503 – 640Pro-richAdd BLAST138

Domaini

The two Pro-rich regions are required for the suppression of AP1 transcription activity.

Sequence similaritiesi

Belongs to the MRL family.Curated
Contains 1 PH domain.PROSITE-ProRule annotation
Contains 1 Ras-associating domain.PROSITE-ProRule annotation

Phylogenomic databases

eggNOGiKOG3751. Eukaryota.
ENOG410XXC8. LUCA.
GeneTreeiENSGT00550000074537.
HOGENOMiHOG000033749.
HOVERGENiHBG080806.
InParanoidiQ7Z5R6.
KOiK17704.
OMAiKVKYKAP.
OrthoDBiEOG091G05PM.
PhylomeDBiQ7Z5R6.
TreeFamiTF317511.

Family and domain databases

Gene3Di2.30.29.30. 1 hit.
InterProiIPR011993. PH_dom-like.
IPR001849. PH_domain.
IPR000159. RA_dom.
IPR029071. Ubiquitin-rel_dom.
[Graphical view]
PfamiPF00169. PH. 1 hit.
PF00788. RA. 1 hit.
[Graphical view]
SMARTiSM00233. PH. 1 hit.
SM00314. RA. 1 hit.
[Graphical view]
SUPFAMiSSF50729. SSF50729. 1 hit.
SSF54236. SSF54236. 1 hit.
PROSITEiPS50003. PH_DOMAIN. 1 hit.
PS50200. RA. 1 hit.
[Graphical view]

Sequences (2)i

Sequence statusi: Complete.

This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 1 (identifier: Q7Z5R6-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MGESSEDIDQ MFSTLLGEMD LLTQSLGVDT LPPPDPNPPR AEFNYSVGFK
60 70 80 90 100
DLNESLNALE DQDLDALMAD LVADISEAEQ RTIQAQKESL QNQHHSASLQ
110 120 130 140 150
ASIFSGAASL GYGTNVAATG ISQYEDDLPP PPADPVLDLP LPPPPPEPLS
160 170 180 190 200
QEEEEAQAKA DKIKLALEKL KEAKVKKLVV KVHMNDNSTK SLMVDERQLA
210 220 230 240 250
RDVLDNLFEK THCDCNVDWC LYEIYPELQI ERFFEDHENV VEVLSDWTRD
260 270 280 290 300
TENKILFLEK EEKYAVFKNP QNFYLDNRGK KESKETNEKM NAKNKESLLE
310 320 330 340 350
ESFCGTSIIV PELEGALYLK EDGKKSWKRR YFLLRASGIY YVPKGKTKTS
360 370 380 390 400
RDLACFIQFE NVNIYYGTQH KMKYKAPTDY CFVLKHPQIQ KESQYIKYLC
410 420 430 440 450
CDDTRTLNQW VMGIRIAKYG KTLYDNYQRA VAKAGLASRW TNLGTVNAAA
460 470 480 490 500
PAQPSTGPKT GTTQPNGQIP QATHSVSAVL QEAQRHAETS KDKKPALGNH
510 520 530 540 550
HDPAVPRAPH APKSSLPPPP PVRRSSDTSG SPATPLKAKG TGGGGLPAPP
560 570 580 590 600
DDFLPPPPPP PPLDDPELPP PPPDFMEPPP DFVPPPPPSY AGIAGSELPP
610 620 630 640 650
PPPPPPAPAP APVPDSARPP PAVAKRPPVP PKRQENPGHP GGAGGGEQDF
660
MSDLMKALQK KRGNVS
Length:666
Mass (Da):73,183
Last modified:October 1, 2003 - v1
Checksum:i525C906C490D8D97
GO
Isoform 2 (identifier: Q7Z5R6-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     152-172: EEEEAQAKADKIKLALEKLKE → VSMWDQRWQDHQPLLPITDVP
     173-666: Missing.

Note: No experimental confirmation available.
Show »
Length:172
Mass (Da):18,686
Checksum:i21314883C4E8C7B9
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti423L → F in BAC41256 (PubMed:14530287).Curated1
Sequence conflicti599Missing in AAN75525 (PubMed:15469846).Curated1

Natural variant

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Natural variantiVAR_050098404T → A.Corresponds to variant rs34081356dbSNPEnsembl.1
Natural variantiVAR_059447617A → T.Corresponds to variant rs7903226dbSNPEnsembl.1

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_056542152 – 172EEEEA…EKLKE → VSMWDQRWQDHQPLLPITDV P in isoform 2. 1 PublicationAdd BLAST21
Alternative sequenceiVSP_056543173 – 666Missing in isoform 2. 1 PublicationAdd BLAST494

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AB085852 mRNA. Translation: BAC41256.1.
AY152730 mRNA. Translation: AAN75525.1.
AL160287 Genomic DNA. Translation: CAH70339.1.
AL355798 Genomic DNA. No translation available.
CH471072 Genomic DNA. Translation: EAW86097.1.
BC035636 mRNA. Translation: AAH35636.1.
BC054516 mRNA. Translation: AAH54516.1.
CCDSiCCDS31167.1. [Q7Z5R6-1]
RefSeqiNP_061916.3. NM_019043.3. [Q7Z5R6-1]
XP_006717514.1. XM_006717451.2. [Q7Z5R6-1]
UniGeneiHs.310421.

Genome annotation databases

EnsembliENST00000356785; ENSP00000349237; ENSG00000077420. [Q7Z5R6-2]
ENST00000376236; ENSP00000365411; ENSG00000077420. [Q7Z5R6-1]
GeneIDi54518.
KEGGihsa:54518.
UCSCiuc001isr.4. human. [Q7Z5R6-1]

Keywords - Coding sequence diversityi

Alternative splicing, Polymorphism

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AB085852 mRNA. Translation: BAC41256.1.
AY152730 mRNA. Translation: AAN75525.1.
AL160287 Genomic DNA. Translation: CAH70339.1.
AL355798 Genomic DNA. No translation available.
CH471072 Genomic DNA. Translation: EAW86097.1.
BC035636 mRNA. Translation: AAH35636.1.
BC054516 mRNA. Translation: AAH54516.1.
CCDSiCCDS31167.1. [Q7Z5R6-1]
RefSeqiNP_061916.3. NM_019043.3. [Q7Z5R6-1]
XP_006717514.1. XM_006717451.2. [Q7Z5R6-1]
UniGeneiHs.310421.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
2MWNNMR-A7-30[»]
3ZDLX-ray2.30B1-31[»]
ProteinModelPortaliQ7Z5R6.
SMRiQ7Z5R6.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi120012. 10 interactors.
IntActiQ7Z5R6. 11 interactors.
STRINGi9606.ENSP00000365411.

PTM databases

iPTMnetiQ7Z5R6.
PhosphoSitePlusiQ7Z5R6.

Polymorphism and mutation databases

BioMutaiAPBB1IP.
DMDMi74750143.

Proteomic databases

EPDiQ7Z5R6.
MaxQBiQ7Z5R6.
PaxDbiQ7Z5R6.
PeptideAtlasiQ7Z5R6.
PRIDEiQ7Z5R6.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENST00000356785; ENSP00000349237; ENSG00000077420. [Q7Z5R6-2]
ENST00000376236; ENSP00000365411; ENSG00000077420. [Q7Z5R6-1]
GeneIDi54518.
KEGGihsa:54518.
UCSCiuc001isr.4. human. [Q7Z5R6-1]

Organism-specific databases

CTDi54518.
DisGeNETi54518.
GeneCardsiAPBB1IP.
H-InvDBHIX0008723.
HIX0035713.
HGNCiHGNC:17379. APBB1IP.
HPAiHPA017009.
HPA063903.
MIMi609036. gene.
neXtProtiNX_Q7Z5R6.
OpenTargetsiENSG00000077420.
PharmGKBiPA134933955.
GenAtlasiSearch...

Phylogenomic databases

eggNOGiKOG3751. Eukaryota.
ENOG410XXC8. LUCA.
GeneTreeiENSGT00550000074537.
HOGENOMiHOG000033749.
HOVERGENiHBG080806.
InParanoidiQ7Z5R6.
KOiK17704.
OMAiKVKYKAP.
OrthoDBiEOG091G05PM.
PhylomeDBiQ7Z5R6.
TreeFamiTF317511.

Enzyme and pathway databases

ReactomeiR-HSA-354192. Integrin alphaIIb beta3 signaling.
R-HSA-354194. GRB2:SOS provides linkage to MAPK signaling for Integrins.
R-HSA-372708. p130Cas linkage to MAPK signaling for integrins.
R-HSA-5674135. MAP2K and MAPK activation.
R-HSA-6802946. Signaling by moderate kinase activity BRAF mutants.
R-HSA-6802948. Signaling by high-kinase activity BRAF mutants.
R-HSA-6802949. Signaling by RAS mutants.
R-HSA-6802952. Signaling by BRAF and RAF fusions.
R-HSA-6802955. Paradoxical activation of RAF signaling by kinase inactive BRAF.

Miscellaneous databases

ChiTaRSiAPBB1IP. human.
GeneWikiiAPBB1IP.
GenomeRNAii54518.
PROiQ7Z5R6.
SOURCEiSearch...

Gene expression databases

BgeeiENSG00000077420.
CleanExiHS_APBB1IP.
GenevisibleiQ7Z5R6. HS.

Family and domain databases

Gene3Di2.30.29.30. 1 hit.
InterProiIPR011993. PH_dom-like.
IPR001849. PH_domain.
IPR000159. RA_dom.
IPR029071. Ubiquitin-rel_dom.
[Graphical view]
PfamiPF00169. PH. 1 hit.
PF00788. RA. 1 hit.
[Graphical view]
SMARTiSM00233. PH. 1 hit.
SM00314. RA. 1 hit.
[Graphical view]
SUPFAMiSSF50729. SSF50729. 1 hit.
SSF54236. SSF54236. 1 hit.
PROSITEiPS50003. PH_DOMAIN. 1 hit.
PS50200. RA. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiAB1IP_HUMAN
AccessioniPrimary (citable) accession number: Q7Z5R6
Secondary accession number(s): Q8IWS8, Q8IYL7, Q8IZZ7
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 22, 2005
Last sequence update: October 1, 2003
Last modified: November 30, 2016
This is version 124 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. Human chromosome 10
    Human chromosome 10: entries, gene names and cross-references to MIM
  2. Human entries with polymorphisms or disease mutations
    List of human entries with polymorphisms or disease mutations
  3. Human polymorphisms and disease mutations
    Index of human polymorphisms and disease mutations
  4. MIM cross-references
    Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
  5. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  6. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.