Reviewed, UniProtKB/Swiss-Prot Q7Z5K2 (WAPL_HUMAN)

Names and origin

Protein names

Recommended name:
    Wings apart-like protein homolog
Alternative name(s):
    Friend of EBNA2 protein

Gene names

Name:	WAPAL
Synonyms:	FOE, KIAA0261, WAPL

Organism

Homo sapiens (Human)

Taxonomic identifier

9606 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Primates › Haplorrhini › Catarrhini › Hominidae › Homo

Protein attributes

Sequence length	1190 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at protein level.

General annotation (Comments)

Function	May play a role in cell growth. Ref.1
Subunit structure	Isoform 2 interacts with Epstein-Barr virus EBNA2. Ref.2
Subcellular location	Isoform 2: Nucleus. Ref.2
Tissue specificity	Isoform 1 is highly expressed in uterine cervix tumor. Isoform 2 is widely espressed with a high level in skeletal muscle and heart. Ref.1 Ref.2
Sequence similarities	Contains 1 WAPL domain.
Sequence caution	The sequence CAH73376.1 differs from that shown. Reason: Erroneous gene model prediction. The sequence CAI16708.1 differs from that shown. Reason: Erroneous gene model prediction.

Ontologies

Keywords
Biological process	Host-virus interaction
Cellular component	Nucleus
Coding sequence diversity	Alternative splicing Polymorphism
Domain	Coiled coil
PTM	Phosphoprotein
Gene Ontology (GO)
Biological process	interspecies interaction between organisms Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	nucleus Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	protein binding Inferred from physical interaction. Source: IntAct
Complete GO annotation...

Binary interactions

With	Entry	#Exp.	IntAct	Notes
PDS5B	Q9NTI5	2	EBI-1022242,EBI-1175604
RAD21	O60216	1	EBI-1022242,EBI-80739
XRN1	Q8IZH2	1	EBI-1022242,EBI-372406

Alternative products

This entry describes 3 isoforms produced by alternative splicing. [Align] [Select]

Note: Named isoforms=2.

Isoform 1 (identifier: Q7Z5K2-1)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

Isoform 2 (identifier: Q7Z5K2-2)

The sequence of this isoform differs from the canonical sequence as follows:
     1-1: M → MVQGPVQTPALTIHRRKRRRRRRRRPVAKAPARLRGEYETGVKM
     510-515: Missing.

Note: No experimental confirmation available.

Isoform 3 (identifier: Q7Z5K2-3)

The sequence of this isoform differs from the canonical sequence as follows:
     1-1: M → MVQGPVQTPA...RGEYETGVKM

Note: No experimental confirmation available.

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 1190	1190	Wings apart-like protein homolog		PRO_0000245232
Regions
	Domain	626 – 1169	544	WAPL
	Coiled coil	260 – 286	27	Potential
	Coiled coil	749 – 782	34	Potential
Amino acid modifications
	Modified residue	77	1	Phosphoserine Ref.6 Ref.7
	Modified residue	221	1	Phosphoserine Ref.6 Ref.7 Ref.9
	Modified residue	223	1	Phosphoserine Ref.7 Ref.8
	Modified residue	226	1	Phosphoserine Ref.6 Ref.7 Ref.9 Ref.5
	Modified residue	380	1	Phosphoserine Ref.7
	Modified residue	459	1	Phosphoserine Ref.7 Ref.9
	Modified residue	461	1	Phosphoserine Ref.7 Ref.9
	Modified residue	904	1	Phosphoserine Ref.7
Natural variations
	Alternative sequence	1	1	M → MVQGPVQTPALTIHRRKRRR RRRRRPVAKAPARLRGEYET GVKM in isoform 2.		VSP_019649
	Alternative sequence	1	1	M → MVQGPVQTPALTIHRRKRRR SCPPNRASYLPKAESLASLG SHLPALLSRARVPRPPAGRR ERERRRRPVAKAPARLRGEY ETGVKM in isoform 3.		VSP_019650
	Alternative sequence	510 – 515	6	Missing in isoform 2.		VSP_019651
	Natural variant	124	1	V → I: dbSNP rs10887621.		VAR_026967

Sequences

	Sequence		Length	Mass (Da)
	Isoform 1 [UniParc]. Last modified October 1, 2003. Version 1. Checksum: A01026E520BFAB2F	FASTA	1,190	132,946
	10 20 30 40 50 60 MTSRFGKTYS RKGGNGSSKF DEVFSNKRTT LSTKWGETTF MAKLGQKRPN FKPDIQEIPK 70 80 90 100 110 120 KPKVEEESTG DPFGFDSDDE SLPVSSKNLA QVKCSSYSES SEAAQLEEVT SVLEANSKIS 130 140 150 160 170 180 HVVVEDTVVS DKCFPLEDTL LGKEKSTNRI VEDDASISSC NKLITSDKVE NFHEEHEKNS 190 200 210 220 230 240 HHIHKNADDS TKKPNAETTV ASEIKETNDT WNSQFGKRPE SPSEISPIKG SVRTGLFEWD 250 260 270 280 290 300 NDFEDIRSED CILSLDSDPL LEMKDDDFKN RLENLNEAIE EDIVQSVLRP TNCRTYCRAN 310 320 330 340 350 360 KTKSSQGASN FDKLMDGTSQ ALAKANSESS KDGLNQAKKG GVSCGTSFRG TVGRTRDYTV 370 380 390 400 410 420 LHPSCLSVCN VTIQDTMERS MDEFTASTPA DLGEAGRLRK KADIATSKTT TRFRPSNTKS 430 440 450 460 470 480 KKDVKLEFFG FEDHETGGDE GGSGSSNYKI KYFGFDDLSE SEDDEDDDCQ VERKTSKKRT 490 500 510 520 530 540 KTAPSPSLQP PPESNDNSQD SQSGTNNAEN LDFTEDLPGV PESVKKPINK QGDKSKENTR 550 560 570 580 590 600 KIFSGPKRSP TKAVYNARHW NHPDSEELPG PPVVKPQSVT VRLSSKEPNQ KDDGVFKAPA 610 620 630 640 650 660 PPSKVIKTVT IPTQPYQDIV TALKCRREDK ELYTVVQHVK HFNDVVEFGE NQEFTDDIEY 670 680 690 700 710 720 LLSGLKSTQP LNTRCLSVIS LATKCAMPSF RMHLRAHGMV AMVFKTLDDS QHHQNLSLCT 730 740 750 760 770 780 AALMYILSRD RLNMDLDRAS LDLMIRLLEL EQDASSAKLL NEKDMNKIKE KIRRLCETVH 790 800 810 820 830 840 NKHLDLENIT TGHLAMETLL SLTSKRAGDW FKEELRLLGG LDHIVDKVKE CVDHLSRDED 850 860 870 880 890 900 EEKLVASLWG AERCLRVLES VTVHNPENQS YLIAYKDSQL IVSSAKALQH CEELIQQYNR 910 920 930 940 950 960 AEDSICLADS KPLPHQNVTN HVGKAVEDCM RAIIGVLLNL TNDNEWGSTK TGEQDGLIGT 970 980 990 1000 1010 1020 ALNCVLQVPK YLPQEQRFDI RVLGLGLLIN LVEYSARNRH CLVNMETSCS FDSSICSGEG 1030 1040 1050 1060 1070 1080 DDSLRIGGQV HAVQALVQLF LERERAAQLA ESKTDELIKD APTTQHDKSG EWQETSGEIQ 1090 1100 1110 1120 1130 1140 WVSTEKTDGT EEKHKKEEED EELDLNKALQ HAGKHMEDCI VASYTALLLG CLCQESPINV 1150 1160 1170 1180 1190 TTVREYLPEG DFSIMTEMLK KFLSFMNLTC AVGTTGQKSI SRVIEYLEHC
	Isoform 2. Checksum: 5431910072375C05	FASTA	1,227	137,314
	10 20 30 40 50 60 MVQGPVQTPA LTIHRRKRRR RRRRRPVAKA PARLRGEYET GVKMTSRFGK TYSRKGGNGS 70 80 90 100 110 120 SKFDEVFSNK RTTLSTKWGE TTFMAKLGQK RPNFKPDIQE IPKKPKVEEE STGDPFGFDS 130 140 150 160 170 180 DDESLPVSSK NLAQVKCSSY SESSEAAQLE EVTSVLEANS KISHVVVEDT VVSDKCFPLE 190 200 210 220 230 240 DTLLGKEKST NRIVEDDASI SSCNKLITSD KVENFHEEHE KNSHHIHKNA DDSTKKPNAE 250 260 270 280 290 300 TTVASEIKET NDTWNSQFGK RPESPSEISP IKGSVRTGLF EWDNDFEDIR SEDCILSLDS 310 320 330 340 350 360 DPLLEMKDDD FKNRLENLNE AIEEDIVQSV LRPTNCRTYC RANKTKSSQG ASNFDKLMDG 370 380 390 400 410 420 TSQALAKANS ESSKDGLNQA KKGGVSCGTS FRGTVGRTRD YTVLHPSCLS VCNVTIQDTM 430 440 450 460 470 480 ERSMDEFTAS TPADLGEAGR LRKKADIATS KTTTRFRPSN TKSKKDVKLE FFGFEDHETG 490 500 510 520 530 540 GDEGGSGSSN YKIKYFGFDD LSESEDDEDD DCQVERKTSK KRTKTAPSPS LQPPPESNDN 550 560 570 580 590 600 SQDSQSGTNN AEDLPGVPES VKKPINKQGD KSKENTRKIF SGPKRSPTKA VYNARHWNHP 610 620 630 640 650 660 DSEELPGPPV VKPQSVTVRL SSKEPNQKDD GVFKAPAPPS KVIKTVTIPT QPYQDIVTAL 670 680 690 700 710 720 KCRREDKELY TVVQHVKHFN DVVEFGENQE FTDDIEYLLS GLKSTQPLNT RCLSVISLAT 730 740 750 760 770 780 KCAMPSFRMH LRAHGMVAMV FKTLDDSQHH QNLSLCTAAL MYILSRDRLN MDLDRASLDL 790 800 810 820 830 840 MIRLLELEQD ASSAKLLNEK DMNKIKEKIR RLCETVHNKH LDLENITTGH LAMETLLSLT 850 860 870 880 890 900 SKRAGDWFKE ELRLLGGLDH IVDKVKECVD HLSRDEDEEK LVASLWGAER CLRVLESVTV 910 920 930 940 950 960 HNPENQSYLI AYKDSQLIVS SAKALQHCEE LIQQYNRAED SICLADSKPL PHQNVTNHVG 970 980 990 1000 1010 1020 KAVEDCMRAI IGVLLNLTND NEWGSTKTGE QDGLIGTALN CVLQVPKYLP QEQRFDIRVL 1030 1040 1050 1060 1070 1080 GLGLLINLVE YSARNRHCLV NMETSCSFDS SICSGEGDDS LRIGGQVHAV QALVQLFLER 1090 1100 1110 1120 1130 1140 ERAAQLAESK TDELIKDAPT TQHDKSGEWQ ETSGEIQWVS TEKTDGTEEK HKKEEEDEEL 1150 1160 1170 1180 1190 1200 DLNKALQHAG KHMEDCIVAS YTALLLGCLC QESPINVTTV REYLPEGDFS IMTEMLKKFL 1210 1220 SFMNLTCAVG TTGQKSISRV IEYLEHC
	Isoform 3. Checksum: 5588D17DBB5C782F	FASTA	1,275	142,525
	10 20 30 40 50 60 MVQGPVQTPA LTIHRRKRRR SCPPNRASYL PKAESLASLG SHLPALLSRA RVPRPPAGRR 70 80 90 100 110 120 ERERRRRPVA KAPARLRGEY ETGVKMTSRF GKTYSRKGGN GSSKFDEVFS NKRTTLSTKW 130 140 150 160 170 180 GETTFMAKLG QKRPNFKPDI QEIPKKPKVE EESTGDPFGF DSDDESLPVS SKNLAQVKCS 190 200 210 220 230 240 SYSESSEAAQ LEEVTSVLEA NSKISHVVVE DTVVSDKCFP LEDTLLGKEK STNRIVEDDA 250 260 270 280 290 300 SISSCNKLIT SDKVENFHEE HEKNSHHIHK NADDSTKKPN AETTVASEIK ETNDTWNSQF 310 320 330 340 350 360 GKRPESPSEI SPIKGSVRTG LFEWDNDFED IRSEDCILSL DSDPLLEMKD DDFKNRLENL 370 380 390 400 410 420 NEAIEEDIVQ SVLRPTNCRT YCRANKTKSS QGASNFDKLM DGTSQALAKA NSESSKDGLN 430 440 450 460 470 480 QAKKGGVSCG TSFRGTVGRT RDYTVLHPSC LSVCNVTIQD TMERSMDEFT ASTPADLGEA 490 500 510 520 530 540 GRLRKKADIA TSKTTTRFRP SNTKSKKDVK LEFFGFEDHE TGGDEGGSGS SNYKIKYFGF 550 560 570 580 590 600 DDLSESEDDE DDDCQVERKT SKKRTKTAPS PSLQPPPESN DNSQDSQSGT NNAENLDFTE 610 620 630 640 650 660 DLPGVPESVK KPINKQGDKS KENTRKIFSG PKRSPTKAVY NARHWNHPDS EELPGPPVVK 670 680 690 700 710 720 PQSVTVRLSS KEPNQKDDGV FKAPAPPSKV IKTVTIPTQP YQDIVTALKC RREDKELYTV 730 740 750 760 770 780 VQHVKHFNDV VEFGENQEFT DDIEYLLSGL KSTQPLNTRC LSVISLATKC AMPSFRMHLR 790 800 810 820 830 840 AHGMVAMVFK TLDDSQHHQN LSLCTAALMY ILSRDRLNMD LDRASLDLMI RLLELEQDAS 850 860 870 880 890 900 SAKLLNEKDM NKIKEKIRRL CETVHNKHLD LENITTGHLA METLLSLTSK RAGDWFKEEL 910 920 930 940 950 960 RLLGGLDHIV DKVKECVDHL SRDEDEEKLV ASLWGAERCL RVLESVTVHN PENQSYLIAY 970 980 990 1000 1010 1020 KDSQLIVSSA KALQHCEELI QQYNRAEDSI CLADSKPLPH QNVTNHVGKA VEDCMRAIIG 1030 1040 1050 1060 1070 1080 VLLNLTNDNE WGSTKTGEQD GLIGTALNCV LQVPKYLPQE QRFDIRVLGL GLLINLVEYS 1090 1100 1110 1120 1130 1140 ARNRHCLVNM ETSCSFDSSI CSGEGDDSLR IGGQVHAVQA LVQLFLERER AAQLAESKTD 1150 1160 1170 1180 1190 1200 ELIKDAPTTQ HDKSGEWQET SGEIQWVSTE KTDGTEEKHK KEEEDEELDL NKALQHAGKH 1210 1220 1230 1240 1250 1260 MEDCIVASYT ALLLGCLCQE SPINVTTVRE YLPEGDFSIM TEMLKKFLSF MNLTCAVGTT 1270 GQKSISRVIE YLEHC

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Expression of a novel human gene, human wings apart-like (hWAPL), is associated with cervical carcinogenesis and tumor progression." Oikawa K., Ohbayashi T., Kiyono T., Nishi H., Isaka K., Umezawa A., Kuroda M., Mukai K. Cancer Res. 64:3545-3549(2004) [PubMed: 15150110] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1), FUNCTION, TISSUE SPECIFICITY.
[2]	"Identification and cloning of a novel chromatin-associated protein partner of Epstein-Barr nuclear protein 2." Kwiatkowski B.A., Ragoczy T., Ehly J., Schubach W.H. Exp. Cell Res. 300:223-233(2004) [PubMed: 15383329] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 2), INTERACTION WITH EPSTEIN-BARR VIRUS EBNA2, SUBCELLULAR LOCATION, TISSUE SPECIFICITY.
[3]	"Prediction of the coding sequences of unidentified human genes. VI. The coding sequences of 80 new genes (KIAA0201-KIAA0280) deduced by analysis of cDNA clones from cell line KG-1 and brain." Nagase T., Seki N., Ishikawa K., Ohira M., Kawarabayasi Y., Ohara O., Tanaka A., Kotani H., Miyajima N., Nomura N. DNA Res. 3:321-329(1996) [PubMed: 9039502] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 3). Tissue: Bone marrow.
[4]	"The DNA sequence and comparative analysis of human chromosome 10." Deloukas P., Earthrowl M.E., Grafham D.V., Rubenfield M., French L., Steward C.A., Sims S.K., Jones M.C., Searle S., Scott C., Howe K., Hunt S.E., Andrews T.D., Gilbert J.G.R., Swarbreck D., Ashurst J.L., Taylor A., Battles J. , Bird C.P., Ainscough R., Almeida J.P., Ashwell R.I.S., Ambrose K.D., Babbage A.K., Bagguley C.L., Bailey J., Banerjee R., Bates K., Beasley H., Bray-Allen S., Brown A.J., Brown J.Y., Burford D.C., Burrill W., Burton J., Cahill P., Camire D., Carter N.P., Chapman J.C., Clark S.Y., Clarke G., Clee C.M., Clegg S., Corby N., Coulson A., Dhami P., Dutta I., Dunn M., Faulkner L., Frankish A., Frankland J.A., Garner P., Garnett J., Gribble S., Griffiths C., Grocock R., Gustafson E., Hammond S., Harley J.L., Hart E., Heath P.D., Ho T.P., Hopkins B., Horne J., Howden P.J., Huckle E., Hynds C., Johnson C., Johnson D., Kana A., Kay M., Kimberley A.M., Kershaw J.K., Kokkinaki M., Laird G.K., Lawlor S., Lee H.M., Leongamornlert D.A., Laird G., Lloyd C., Lloyd D.M., Loveland J., Lovell J., McLaren S., McLay K.E., McMurray A., Mashreghi-Mohammadi M., Matthews L., Milne S., Nickerson T., Nguyen M., Overton-Larty E., Palmer S.A., Pearce A.V., Peck A.I., Pelan S., Phillimore B., Porter K., Rice C.M., Rogosin A., Ross M.T., Sarafidou T., Sehra H.K., Shownkeen R., Skuce C.D., Smith M., Standring L., Sycamore N., Tester J., Thorpe A., Torcasso W., Tracey A., Tromans A., Tsolas J., Wall M., Walsh J., Wang H., Weinstock K., West A.P., Willey D.L., Whitehead S.L., Wilming L., Wray P.W., Young L., Chen Y., Lovering R.C., Moschonas N.K., Siebert R., Fechtel K., Bentley D., Durbin R.M., Hubbard T., Doucette-Stamm L., Beck S., Smith D.R., Rogers J. Nature 429:375-381(2004) [PubMed: 15164054] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[5]	"Automated phosphoproteome analysis for cultured cancer cells by two-dimensional nanoLC-MS using a calcined titania/C18 biphasic column." Imami K., Sugiyama N., Kyono Y., Tomita M., Ishihama Y. Anal. Sci. 24:161-166(2008) [PubMed: 18187866] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-226, MASS SPECTROMETRY.
[6]	"Kinase-selective enrichment enables quantitative phosphoproteomics of the kinome across the cell cycle." Daub H., Olsen J.V., Bairlein M., Gnad F., Oppermann F.S., Korner R., Greff Z., Keri G., Stemmann O., Mann M. Mol. Cell 31:438-448(2008) [PubMed: 18691976] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-77; SER-221 AND SER-226, MASS SPECTROMETRY.
[7]	"A quantitative atlas of mitotic phosphorylation." Dephoure N., Zhou C., Villen J., Beausoleil S.A., Bakalarski C.E., Elledge S.J., Gygi S.P. Proc. Natl. Acad. Sci. U.S.A. 105:10762-10767(2008) [PubMed: 18669648] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-77; SER-221; SER-223; SER-226; SER-380; SER-459; SER-461 AND SER-904, MASS SPECTROMETRY.
[8]	Carrascal M., Abian J. Submitted (JAN-2008) to UniProtKB Cited for: PHOSPHORYLATION AT SER-223, MASS SPECTROMETRY. Tissue: T-cell.
[9]	"Global, in vivo, and site-specific phosphorylation dynamics in signaling networks." Olsen J.V., Blagoev B., Gnad F., Macek B., Kumar C., Mortensen P., Mann M. Cell 127:635-648(2006) [PubMed: 17081983] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-221; SER-226; SER-459 AND SER-461, MASS SPECTROMETRY. Tissue: Epithelium.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBLGenBankDDBJ	AB065003 mRNA. Translation: BAC78631.1. AF479418 mRNA. Translation: AAO14651.1. D87450 mRNA. Translation: BAA13391.1. Different initiation. AL844892, AL731569 Genomic DNA. Translation: CAH73376.1. Sequence problems. AL731569, AL844892 Genomic DNA. Translation: CAI16708.1. Sequence problems.
IPI	IPI00375330. IPI00412441. IPI00744918.
RefSeq	NP_055860.1.
UniGene	Hs.203099 Hs.714876
3D structure databases
ModBase	Search...
Protein-protein interaction databases
IntAct	Q7Z5K2. 8 interactions.
PTM databases
PhosphoSite	Q7Z5K2.
Proteomic databases
PRIDE	Q7Z5K2.
Genome annotation databases
Ensembl	ENSG00000062650. Homo sapiens. [Contig view]
GeneID	23063.
KEGG	hsa:23063.
Organism-specific databases
GeneCards	GC10M088185.
HGNC	HGNC:23293. WAPAL.
MIM	610754. gene.
HUGE	Search...
GenAtlas	Search...
Phylogenomic databases
HOGENOM	Q7Z5K2.
HOVERGEN	Q7Z5K2.
OMA	Q7Z5K2. IKGSVRT.
Gene expression databases
ArrayExpress	Q7Z5K2.
Bgee	Q7Z5K2.
CleanEx	HS_WAPAL.
GermOnline	ENSG00000062650. Homo sapiens.
Family and domain databases
InterPro	IPR012502. WAPL. [Graphical view]
Pfam	PF07814. WAPL. 1 hit. [Graphical view]
PROSITE	PS51271. WAPL. 1 hit. [Graphical view]
ProtoNet	Search...
Other Resources
NextBio	44141.
SOURCE	Search...

Entry information

Entry name

WAPL_HUMAN

Accession

Primary (citable) accession number: Q7Z5K2
Secondary accession number(s): Q5VSK5, Q8IX10, Q92549

Entry history

Integrated into UniProtKB/Swiss-Prot:	July 11, 2006
Last sequence update:	October 1, 2003
Last modified:	June 16, 2009
This is version 42 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HPI (Human Proteome Initiative)