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Protein

Rho GTPase-activating protein 22

Gene

ARHGAP22

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Rho GTPase-activating protein involved in the signal transduction pathway that regulates endothelial cell capillary tube formation during angiogenesis. Acts as a GTPase activator for the RAC1 by converting it to an inactive GDP-bound state. Inhibits RAC1-dependent lamellipodia formation. May also play a role in transcription regulation via its interaction with VEZF1, by regulating activity of the endothelin-1 (EDN1) promoter (By similarity).By similarity

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Developmental protein, GTPase activation

Keywords - Biological processi

Angiogenesis, Differentiation, Transcription, Transcription regulation

Enzyme and pathway databases

ReactomeiR-HSA-194840. Rho GTPase cycle.

Names & Taxonomyi

Protein namesi
Recommended name:
Rho GTPase-activating protein 22
Alternative name(s):
Rho-type GTPase-activating protein 22
Gene namesi
Name:ARHGAP22
Synonyms:RHOGAP2
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
Proteomesi
  • UP000005640 Componenti: Chromosome 10

Organism-specific databases

HGNCiHGNC:30320. ARHGAP22.

Subcellular locationi

  • Cytoplasm By similarity
  • Nucleus By similarity

  • Note: Mainly cytoplasmic. Some fraction is nuclear (By similarity).By similarity

GO - Cellular componenti

  • cytosol Source: Reactome
  • focal adhesion Source: UniProtKB
  • nucleus Source: UniProtKB-SubCell
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm, Nucleus

Pathology & Biotechi

Organism-specific databases

DisGeNETi58504.
OpenTargetsiENSG00000128805.
PharmGKBiPA134979724.

Polymorphism and mutation databases

BioMutaiARHGAP22.
DMDMi74750129.

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00002804691 – 698Rho GTPase-activating protein 22Add BLAST698

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei359PhosphoserineCombined sources1
Modified residuei395PhosphoserineCombined sources1

Keywords - PTMi

Phosphoprotein

Proteomic databases

MaxQBiQ7Z5H3.
PaxDbiQ7Z5H3.
PeptideAtlasiQ7Z5H3.
PRIDEiQ7Z5H3.

PTM databases

iPTMnetiQ7Z5H3.
PhosphoSitePlusiQ7Z5H3.

Expressioni

Gene expression databases

BgeeiENSG00000128805.
CleanExiHS_ARHGAP22.
ExpressionAtlasiQ7Z5H3. baseline and differential.
GenevisibleiQ7Z5H3. HS.

Organism-specific databases

HPAiCAB020696.
HPA040391.
HPA046797.
HPA064439.

Interactioni

Subunit structurei

Interacts with VEZF1.By similarity

Binary interactionsi

WithEntry#Exp.IntActNotes
CACNA1AO005552EBI-3866859,EBI-766279
CCNDBP1O952733EBI-3866859,EBI-748961

Protein-protein interaction databases

BioGridi121832. 30 interactors.
IntActiQ7Z5H3. 3 interactors.
STRINGi9606.ENSP00000249601.

Structurei

3D structure databases

ProteinModelPortaliQ7Z5H3.
SMRiQ7Z5H3.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini37 – 145PHPROSITE-ProRule annotationAdd BLAST109
Domaini155 – 349Rho-GAPPROSITE-ProRule annotationAdd BLAST195

Coiled coil

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Coiled coili590 – 687Sequence analysisAdd BLAST98

Compositional bias

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Compositional biasi427 – 587Ser-richAdd BLAST161

Sequence similaritiesi

Contains 1 PH domain.PROSITE-ProRule annotation
Contains 1 Rho-GAP domain.PROSITE-ProRule annotation

Keywords - Domaini

Coiled coil

Phylogenomic databases

eggNOGiKOG4270. Eukaryota.
ENOG410XRR2. LUCA.
GeneTreeiENSGT00760000118863.
HOGENOMiHOG000232151.
HOVERGENiHBG058875.
InParanoidiQ7Z5H3.
KOiK20642.
OMAiGEREKVP.
OrthoDBiEOG091G02DD.
PhylomeDBiQ7Z5H3.
TreeFamiTF323577.

Family and domain databases

Gene3Di1.10.555.10. 1 hit.
2.30.29.30. 1 hit.
InterProiIPR011993. PH_dom-like.
IPR001849. PH_domain.
IPR008936. Rho_GTPase_activation_prot.
IPR000198. RhoGAP_dom.
[Graphical view]
PfamiPF00169. PH. 1 hit.
PF00620. RhoGAP. 1 hit.
[Graphical view]
SMARTiSM00233. PH. 1 hit.
SM00324. RhoGAP. 1 hit.
[Graphical view]
SUPFAMiSSF48350. SSF48350. 1 hit.
SSF50729. SSF50729. 1 hit.
PROSITEiPS50003. PH_DOMAIN. 1 hit.
PS50238. RHOGAP. 1 hit.
[Graphical view]

Sequences (5)i

Sequence statusi: Complete.

This entry describes 5 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 1 (identifier: Q7Z5H3-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MLSPKIRQAR RARSKSLVMG EQSRSPGRMP CPHRLGPVLK AGWLKKQRSI
60 70 80 90 100
MKNWQQRWFV LRGDQLFYYK DKDEIKPQGF ISLQGTQVTE LPPGPEDPGK
110 120 130 140 150
HLFEISPGGA GEREKVPANP EALLLMASSQ RDMEDWVQAI RRVIWAPLGG
160 170 180 190 200
GIFGQRLEET VHHERKYGPR LAPLLVEQCV DFIRERGLTE EGLFRMPGQA
210 220 230 240 250
NLVRDLQDSF DCGEKPLFDS TTDVHTVASL LKLYLRELPE PVVPFARYED
260 270 280 290 300
FLSCAQLLTK DEGEGTLELA KQVSNLPQAN YNLLRYICKF LDEVQAYSNV
310 320 330 340 350
NKMSVQNLAT VFGPNILRPQ VEDPVTIMEG TSLVQHLMTV LIRKHSQLFT
360 370 380 390 400
APVPEGPTSP RGGLQCAVGW GSEEVTRDSQ GEPGGPGLPA HRTSSLDGAA
410 420 430 440 450
VAVLSRTAPT GPGSRCSPGK KVQTLPSWKS SFRQPRSLSG SPKGGGSSLE
460 470 480 490 500
VPIISSGGNW LMNGLSSLRG HRRASSGDRL KDSGSVQRLS TYDNVPAPGL
510 520 530 540 550
VPGIPSVASM AWSGASSSES SVGGSLSSCT ACRASDSSAR SSLHTDWALE
560 570 580 590 600
PSPLPSSSED PKSLDLDHSM DEAGAGASNS EPSEPDSPTR EHARRSEALQ
610 620 630 640 650
GLVTELRAEL CRQRTEYERS VKRIEEGSAD LRKRMSRLEE ELDQEKKKYI
660 670 680 690
MLEIKLRNSE RAREDAERRN QLLQREMEEF FSTLGSLTVG AKGARAPK
Length:698
Mass (Da):76,779
Last modified:October 1, 2003 - v1
Checksum:i3CE8CEB0A9E5D0F8
GO
Isoform 2 (identifier: Q7Z5H3-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     150-150: G → GGTARRSHAHPLEPLPP

Note: No experimental confirmation available.
Show »
Length:714
Mass (Da):78,497
Checksum:i74AB4A00D66DE416
GO
Isoform 3 (identifier: Q7Z5H3-3) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     1-107: MLSPKIRQAR...PGKHLFEISP → MPFWPIRCLKRSRRMPR

Note: No experimental confirmation available.
Show »
Length:608
Mass (Da):66,611
Checksum:i2D7543609C576A5D
GO
Isoform 4 (identifier: Q7Z5H3-4) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     1-329: MLSPKIRQAR...QVEDPVTIME → MGQPREFWHL...QGPSPPLFPA

Note: No experimental confirmation available.
Show »
Length:494
Mass (Da):52,697
Checksum:i2E77D20B3241012D
GO
Isoform 5 (identifier: Q7Z5H3-5) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     1-12: MLSPKIRQARRA → MLPTASSKRRTFAARYFT

Note: No experimental confirmation available.
Show »
Length:704
Mass (Da):77,457
Checksum:iBC4DB2D59C466994
GO

Natural variant

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Natural variantiVAR_031153410T → K.Corresponds to variant rs1867586dbSNPEnsembl.1
Natural variantiVAR_031154612R → C.Corresponds to variant rs3747853dbSNPEnsembl.1

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_0237031 – 329MLSPK…VTIME → MGQPREFWHLRSQVPVPTCH LLTVQPWCLLPHPTPAGGRE RRHSASSLLAALCLRKAVLR PHPAVLPGVGGCVVLSSGRM KAPLRGGLALGTGPAPTKLQ LLHDWQSEPGPSGWQQGPSP PLFPA in isoform 4. 1 PublicationAdd BLAST329
Alternative sequenceiVSP_0237041 – 107MLSPK…FEISP → MPFWPIRCLKRSRRMPR in isoform 3. 1 PublicationAdd BLAST107
Alternative sequenceiVSP_0535021 – 12MLSPK…QARRA → MLPTASSKRRTFAARYFT in isoform 5. 1 PublicationAdd BLAST12
Alternative sequenceiVSP_023705150G → GGTARRSHAHPLEPLPP in isoform 2. 1 Publication1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AY324801 mRNA. Translation: AAP85632.1.
AK127586 mRNA. Translation: BAC87044.1.
AK293579 mRNA. Translation: BAG57049.1.
U90908 mRNA. Translation: AAB51057.1.
EF560749 mRNA. Translation: ABQ59059.1.
AC016397 Genomic DNA. No translation available.
AC016399 Genomic DNA. No translation available.
AC068898 Genomic DNA. No translation available.
CH471187 Genomic DNA. Translation: EAW93127.1.
BC126444 mRNA. Translation: AAI26445.1.
BC136319 mRNA. Translation: AAI36320.1.
CCDSiCCDS58079.1. [Q7Z5H3-3]
CCDS58080.1. [Q7Z5H3-2]
CCDS58081.1. [Q7Z5H3-5]
CCDS7227.1. [Q7Z5H3-1]
RefSeqiNP_001242953.1. NM_001256024.1. [Q7Z5H3-2]
NP_001242954.1. NM_001256025.2. [Q7Z5H3-5]
NP_001242955.1. NM_001256026.1. [Q7Z5H3-3]
NP_067049.2. NM_021226.3. [Q7Z5H3-1]
XP_011538306.1. XM_011540004.1. [Q7Z5H3-5]
UniGeneiHs.655672.
Hs.680368.
Hs.740750.

Genome annotation databases

EnsembliENST00000249601; ENSP00000249601; ENSG00000128805. [Q7Z5H3-1]
ENST00000417247; ENSP00000410054; ENSG00000128805. [Q7Z5H3-3]
ENST00000417912; ENSP00000412461; ENSG00000128805. [Q7Z5H3-2]
ENST00000435790; ENSP00000416701; ENSG00000128805. [Q7Z5H3-5]
GeneIDi58504.
KEGGihsa:58504.
UCSCiuc001jgs.5. human. [Q7Z5H3-1]

Keywords - Coding sequence diversityi

Alternative splicing, Polymorphism

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AY324801 mRNA. Translation: AAP85632.1.
AK127586 mRNA. Translation: BAC87044.1.
AK293579 mRNA. Translation: BAG57049.1.
U90908 mRNA. Translation: AAB51057.1.
EF560749 mRNA. Translation: ABQ59059.1.
AC016397 Genomic DNA. No translation available.
AC016399 Genomic DNA. No translation available.
AC068898 Genomic DNA. No translation available.
CH471187 Genomic DNA. Translation: EAW93127.1.
BC126444 mRNA. Translation: AAI26445.1.
BC136319 mRNA. Translation: AAI36320.1.
CCDSiCCDS58079.1. [Q7Z5H3-3]
CCDS58080.1. [Q7Z5H3-2]
CCDS58081.1. [Q7Z5H3-5]
CCDS7227.1. [Q7Z5H3-1]
RefSeqiNP_001242953.1. NM_001256024.1. [Q7Z5H3-2]
NP_001242954.1. NM_001256025.2. [Q7Z5H3-5]
NP_001242955.1. NM_001256026.1. [Q7Z5H3-3]
NP_067049.2. NM_021226.3. [Q7Z5H3-1]
XP_011538306.1. XM_011540004.1. [Q7Z5H3-5]
UniGeneiHs.655672.
Hs.680368.
Hs.740750.

3D structure databases

ProteinModelPortaliQ7Z5H3.
SMRiQ7Z5H3.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi121832. 30 interactors.
IntActiQ7Z5H3. 3 interactors.
STRINGi9606.ENSP00000249601.

PTM databases

iPTMnetiQ7Z5H3.
PhosphoSitePlusiQ7Z5H3.

Polymorphism and mutation databases

BioMutaiARHGAP22.
DMDMi74750129.

Proteomic databases

MaxQBiQ7Z5H3.
PaxDbiQ7Z5H3.
PeptideAtlasiQ7Z5H3.
PRIDEiQ7Z5H3.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENST00000249601; ENSP00000249601; ENSG00000128805. [Q7Z5H3-1]
ENST00000417247; ENSP00000410054; ENSG00000128805. [Q7Z5H3-3]
ENST00000417912; ENSP00000412461; ENSG00000128805. [Q7Z5H3-2]
ENST00000435790; ENSP00000416701; ENSG00000128805. [Q7Z5H3-5]
GeneIDi58504.
KEGGihsa:58504.
UCSCiuc001jgs.5. human. [Q7Z5H3-1]

Organism-specific databases

CTDi58504.
DisGeNETi58504.
GeneCardsiARHGAP22.
HGNCiHGNC:30320. ARHGAP22.
HPAiCAB020696.
HPA040391.
HPA046797.
HPA064439.
MIMi610585. gene.
neXtProtiNX_Q7Z5H3.
OpenTargetsiENSG00000128805.
PharmGKBiPA134979724.
GenAtlasiSearch...

Phylogenomic databases

eggNOGiKOG4270. Eukaryota.
ENOG410XRR2. LUCA.
GeneTreeiENSGT00760000118863.
HOGENOMiHOG000232151.
HOVERGENiHBG058875.
InParanoidiQ7Z5H3.
KOiK20642.
OMAiGEREKVP.
OrthoDBiEOG091G02DD.
PhylomeDBiQ7Z5H3.
TreeFamiTF323577.

Enzyme and pathway databases

ReactomeiR-HSA-194840. Rho GTPase cycle.

Miscellaneous databases

ChiTaRSiARHGAP22. human.
GenomeRNAii58504.
PROiQ7Z5H3.
SOURCEiSearch...

Gene expression databases

BgeeiENSG00000128805.
CleanExiHS_ARHGAP22.
ExpressionAtlasiQ7Z5H3. baseline and differential.
GenevisibleiQ7Z5H3. HS.

Family and domain databases

Gene3Di1.10.555.10. 1 hit.
2.30.29.30. 1 hit.
InterProiIPR011993. PH_dom-like.
IPR001849. PH_domain.
IPR008936. Rho_GTPase_activation_prot.
IPR000198. RhoGAP_dom.
[Graphical view]
PfamiPF00169. PH. 1 hit.
PF00620. RhoGAP. 1 hit.
[Graphical view]
SMARTiSM00233. PH. 1 hit.
SM00324. RhoGAP. 1 hit.
[Graphical view]
SUPFAMiSSF48350. SSF48350. 1 hit.
SSF50729. SSF50729. 1 hit.
PROSITEiPS50003. PH_DOMAIN. 1 hit.
PS50238. RHOGAP. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiRHG22_HUMAN
AccessioniPrimary (citable) accession number: Q7Z5H3
Secondary accession number(s): A0AVP7
, A5YM75, B4DED8, B9EGA0, C9JDM2, O00152, Q6ZSB0
Entry historyi
Integrated into UniProtKB/Swiss-Prot: March 20, 2007
Last sequence update: October 1, 2003
Last modified: November 30, 2016
This is version 111 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Human chromosome 10
    Human chromosome 10: entries, gene names and cross-references to MIM
  2. Human entries with polymorphisms or disease mutations
    List of human entries with polymorphisms or disease mutations
  3. Human polymorphisms and disease mutations
    Index of human polymorphisms and disease mutations
  4. MIM cross-references
    Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
  5. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.