ID   TAFA5_HUMAN             Reviewed;         132 AA.
AC   Q7Z5A7; A6NII9; B0QZ13; B0QZ14; B0QZ15; O95902; Q5H9C4; Q6UWC9; Q8IXR8;
DT   08-NOV-2005, integrated into UniProtKB/Swiss-Prot.
DT   02-SEP-2008, sequence version 2.
DT   27-MAR-2024, entry version 133.
DE   RecName: Full=Chemokine-like protein TAFA-5 {ECO:0000305};
DE   Flags: Precursor;
GN   Name=TAFA5 {ECO:0000312|HGNC:HGNC:21592};
GN   Synonyms=FAM19A5 {ECO:0000312|HGNC:HGNC:21592};
GN   ORFNames=UNQ5208/PRO34524;
OS   Homo sapiens (Human).
OC   Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC   Eutheria; Euarchontoglires; Primates; Haplorrhini; Catarrhini; Hominidae;
OC   Homo.
OX   NCBI_TaxID=9606;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 2), TISSUE SPECIFICITY, AND SUBCELLULAR
RP   LOCATION.
RX   PubMed=15028294; DOI=10.1016/j.ygeno.2003.10.006;
RA   Tom Tang Y., Emtage P., Funk W.D., Hu T., Arterburn M., Park E.E., Rupp F.;
RT   "TAFA: a novel secreted family with conserved cysteine residues and
RT   restricted expression in the brain.";
RL   Genomics 83:727-734(2004).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
RC   TISSUE=Brain;
RA   Mei G., Yu W., Gibbs R.A.;
RL   Submitted (FEB-1999) to the EMBL/GenBank/DDBJ databases.
RN   [3]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 2).
RX   PubMed=12975309; DOI=10.1101/gr.1293003;
RA   Clark H.F., Gurney A.L., Abaya E., Baker K., Baldwin D.T., Brush J.,
RA   Chen J., Chow B., Chui C., Crowley C., Currell B., Deuel B., Dowd P.,
RA   Eaton D., Foster J.S., Grimaldi C., Gu Q., Hass P.E., Heldens S., Huang A.,
RA   Kim H.S., Klimowski L., Jin Y., Johnson S., Lee J., Lewis L., Liao D.,
RA   Mark M.R., Robbie E., Sanchez C., Schoenfeld J., Seshagiri S., Simmons L.,
RA   Singh J., Smith V., Stinson J., Vagts A., Vandlen R.L., Watanabe C.,
RA   Wieand D., Woods K., Xie M.-H., Yansura D.G., Yi S., Yu G., Yuan J.,
RA   Zhang M., Zhang Z., Goddard A.D., Wood W.I., Godowski P.J., Gray A.M.;
RT   "The secreted protein discovery initiative (SPDI), a large-scale effort to
RT   identify novel human secreted and transmembrane proteins: a bioinformatics
RT   assessment.";
RL   Genome Res. 13:2265-2270(2003).
RN   [4]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 2).
RC   TISSUE=Neuroblastoma, and Subthalamic nucleus;
RX   PubMed=14702039; DOI=10.1038/ng1285;
RA   Ota T., Suzuki Y., Nishikawa T., Otsuki T., Sugiyama T., Irie R.,
RA   Wakamatsu A., Hayashi K., Sato H., Nagai K., Kimura K., Makita H.,
RA   Sekine M., Obayashi M., Nishi T., Shibahara T., Tanaka T., Ishii S.,
RA   Yamamoto J., Saito K., Kawai Y., Isono Y., Nakamura Y., Nagahari K.,
RA   Murakami K., Yasuda T., Iwayanagi T., Wagatsuma M., Shiratori A., Sudo H.,
RA   Hosoiri T., Kaku Y., Kodaira H., Kondo H., Sugawara M., Takahashi M.,
RA   Kanda K., Yokoi T., Furuya T., Kikkawa E., Omura Y., Abe K., Kamihara K.,
RA   Katsuta N., Sato K., Tanikawa M., Yamazaki M., Ninomiya K., Ishibashi T.,
RA   Yamashita H., Murakawa K., Fujimori K., Tanai H., Kimata M., Watanabe M.,
RA   Hiraoka S., Chiba Y., Ishida S., Ono Y., Takiguchi S., Watanabe S.,
RA   Yosida M., Hotuta T., Kusano J., Kanehori K., Takahashi-Fujii A., Hara H.,
RA   Tanase T.-O., Nomura Y., Togiya S., Komai F., Hara R., Takeuchi K.,
RA   Arita M., Imose N., Musashino K., Yuuki H., Oshima A., Sasaki N.,
RA   Aotsuka S., Yoshikawa Y., Matsunawa H., Ichihara T., Shiohata N., Sano S.,
RA   Moriya S., Momiyama H., Satoh N., Takami S., Terashima Y., Suzuki O.,
RA   Nakagawa S., Senoh A., Mizoguchi H., Goto Y., Shimizu F., Wakebe H.,
RA   Hishigaki H., Watanabe T., Sugiyama A., Takemoto M., Kawakami B.,
RA   Yamazaki M., Watanabe K., Kumagai A., Itakura S., Fukuzumi Y., Fujimori Y.,
RA   Komiyama M., Tashiro H., Tanigami A., Fujiwara T., Ono T., Yamada K.,
RA   Fujii Y., Ozaki K., Hirao M., Ohmori Y., Kawabata A., Hikiji T.,
RA   Kobatake N., Inagaki H., Ikema Y., Okamoto S., Okitani R., Kawakami T.,
RA   Noguchi S., Itoh T., Shigeta K., Senba T., Matsumura K., Nakajima Y.,
RA   Mizuno T., Morinaga M., Sasaki M., Togashi T., Oyama M., Hata H.,
RA   Watanabe M., Komatsu T., Mizushima-Sugano J., Satoh T., Shirai Y.,
RA   Takahashi Y., Nakagawa K., Okumura K., Nagase T., Nomura N., Kikuchi H.,
RA   Masuho Y., Yamashita R., Nakai K., Yada T., Nakamura Y., Ohara O.,
RA   Isogai T., Sugano S.;
RT   "Complete sequencing and characterization of 21,243 full-length human
RT   cDNAs.";
RL   Nat. Genet. 36:40-45(2004).
RN   [5]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RX   PubMed=10591208; DOI=10.1038/990031;
RA   Dunham I., Hunt A.R., Collins J.E., Bruskiewich R., Beare D.M., Clamp M.,
RA   Smink L.J., Ainscough R., Almeida J.P., Babbage A.K., Bagguley C.,
RA   Bailey J., Barlow K.F., Bates K.N., Beasley O.P., Bird C.P., Blakey S.E.,
RA   Bridgeman A.M., Buck D., Burgess J., Burrill W.D., Burton J., Carder C.,
RA   Carter N.P., Chen Y., Clark G., Clegg S.M., Cobley V.E., Cole C.G.,
RA   Collier R.E., Connor R., Conroy D., Corby N.R., Coville G.J., Cox A.V.,
RA   Davis J., Dawson E., Dhami P.D., Dockree C., Dodsworth S.J., Durbin R.M.,
RA   Ellington A.G., Evans K.L., Fey J.M., Fleming K., French L., Garner A.A.,
RA   Gilbert J.G.R., Goward M.E., Grafham D.V., Griffiths M.N.D., Hall C.,
RA   Hall R.E., Hall-Tamlyn G., Heathcott R.W., Ho S., Holmes S., Hunt S.E.,
RA   Jones M.C., Kershaw J., Kimberley A.M., King A., Laird G.K., Langford C.F.,
RA   Leversha M.A., Lloyd C., Lloyd D.M., Martyn I.D., Mashreghi-Mohammadi M.,
RA   Matthews L.H., Mccann O.T., Mcclay J., Mclaren S., McMurray A.A.,
RA   Milne S.A., Mortimore B.J., Odell C.N., Pavitt R., Pearce A.V., Pearson D.,
RA   Phillimore B.J.C.T., Phillips S.H., Plumb R.W., Ramsay H., Ramsey Y.,
RA   Rogers L., Ross M.T., Scott C.E., Sehra H.K., Skuce C.D., Smalley S.,
RA   Smith M.L., Soderlund C., Spragon L., Steward C.A., Sulston J.E.,
RA   Swann R.M., Vaudin M., Wall M., Wallis J.M., Whiteley M.N., Willey D.L.,
RA   Williams L., Williams S.A., Williamson H., Wilmer T.E., Wilming L.,
RA   Wright C.L., Hubbard T., Bentley D.R., Beck S., Rogers J., Shimizu N.,
RA   Minoshima S., Kawasaki K., Sasaki T., Asakawa S., Kudoh J., Shintani A.,
RA   Shibuya K., Yoshizaki Y., Aoki N., Mitsuyama S., Roe B.A., Chen F., Chu L.,
RA   Crabtree J., Deschamps S., Do A., Do T., Dorman A., Fang F., Fu Y., Hu P.,
RA   Hua A., Kenton S., Lai H., Lao H.I., Lewis J., Lewis S., Lin S.-P., Loh P.,
RA   Malaj E., Nguyen T., Pan H., Phan S., Qi S., Qian Y., Ray L., Ren Q.,
RA   Shaull S., Sloan D., Song L., Wang Q., Wang Y., Wang Z., White J.,
RA   Willingham D., Wu H., Yao Z., Zhan M., Zhang G., Chissoe S., Murray J.,
RA   Miller N., Minx P., Fulton R., Johnson D., Bemis G., Bentley D.,
RA   Bradshaw H., Bourne S., Cordes M., Du Z., Fulton L., Goela D., Graves T.,
RA   Hawkins J., Hinds K., Kemp K., Latreille P., Layman D., Ozersky P.,
RA   Rohlfing T., Scheet P., Walker C., Wamsley A., Wohldmann P., Pepin K.,
RA   Nelson J., Korf I., Bedell J.A., Hillier L.W., Mardis E., Waterston R.,
RA   Wilson R., Emanuel B.S., Shaikh T., Kurahashi H., Saitta S., Budarf M.L.,
RA   McDermid H.E., Johnson A., Wong A.C.C., Morrow B.E., Edelmann L., Kim U.J.,
RA   Shizuya H., Simon M.I., Dumanski J.P., Peyrard M., Kedra D., Seroussi E.,
RA   Fransson I., Tapia I., Bruder C.E., O'Brien K.P., Wilkinson P.,
RA   Bodenteich A., Hartman K., Hu X., Khan A.S., Lane L., Tilahun Y.,
RA   Wright H.;
RT   "The DNA sequence of human chromosome 22.";
RL   Nature 402:489-495(1999).
RN   [6]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
RC   TISSUE=Hypothalamus;
RX   PubMed=15489334; DOI=10.1101/gr.2596504;
RG   The MGC Project Team;
RT   "The status, quality, and expansion of the NIH full-length cDNA project:
RT   the Mammalian Gene Collection (MGC).";
RL   Genome Res. 14:2121-2127(2004).
RN   [7]
RP   PROTEIN SEQUENCE OF 44-53 (ISOFORMS 1 AND 2), SUBCELLULAR LOCATION, AND
RP   TISSUE SPECIFICITY.
RX   PubMed=29453251; DOI=10.1161/circulationaha.117.032398;
RA   Wang Y., Chen D., Zhang Y., Wang P., Zheng C., Zhang S., Yu B., Zhang L.,
RA   Zhao G., Ma B., Cai Z., Xie N., Huang S., Liu Z., Mo X., Guan Y., Wang X.,
RA   Fu Y., Ma D., Wang Y., Kong W.;
RT   "Novel Adipokine, FAM19A5, Inhibits Neointima Formation After Injury
RT   Through Sphingosine-1-Phosphate Receptor 2.";
RL   Circulation 138:48-63(2018).
CC   -!- FUNCTION: Acts as a chemokine-like protein by regulating cell
CC       proliferation and migration through activation of G protein-coupled
CC       receptors (GPCRs), such as S1PR2 and FPR2 (By similarity). Stimulates
CC       chemotactic migration of macrophages mediated by the MAPK3/ERK1 and
CC       AKT1 pathway (By similarity). Blocks TNFSF11/RANKL-induced osteoclast
CC       formation from macrophages by inhibiting up-regulation of osteoclast
CC       fusogenic and differentiation genes (By similarity). Stimulation of
CC       macrophage migration and inhibition of osteoclast formation is mediated
CC       via GPCR FPR2 (By similarity). Acts as an adipokine by negatively
CC       regulating vascular smooth muscle cell (VSMC) proliferation and
CC       migration in response to platelet-derived growth factor stimulation via
CC       GPCR S1PR2 and G protein GNA12/GNA13-transmitted RHOA signaling (By
CC       similarity). Inhibits injury-induced cell proliferation and neointima
CC       formation in the femoral arteries (By similarity).
CC       {ECO:0000250|UniProtKB:M0R7X9, ECO:0000250|UniProtKB:Q91WE9}.
CC   -!- SUBCELLULAR LOCATION: Secreted {ECO:0000269|PubMed:15028294,
CC       ECO:0000269|PubMed:29453251}.
CC   -!- ALTERNATIVE PRODUCTS:
CC       Event=Alternative splicing; Named isoforms=3;
CC       Name=1;
CC         IsoId=Q7Z5A7-1; Sequence=Displayed;
CC       Name=2;
CC         IsoId=Q7Z5A7-2; Sequence=VSP_016068;
CC       Name=3;
CC         IsoId=Q7Z5A7-3; Sequence=VSP_035191;
CC   -!- TISSUE SPECIFICITY: Expressed in the subcutaneous and perirenal adipose
CC       tissue (at protein level) (PubMed:29453251). Highly expressed in
CC       adipose tissue with moderate expression in the brain and ovary
CC       (PubMed:29453251). Isoform 2: Brain-specific (PubMed:15028294).
CC       {ECO:0000269|PubMed:15028294, ECO:0000269|PubMed:29453251}.
CC   -!- MISCELLANEOUS: [Isoform 2]: Contains a predicted signal peptide at
CC       positions 1-25. {ECO:0000305}.
CC   -!- SIMILARITY: Belongs to the TAFA family. {ECO:0000305}.
CC   -!- SEQUENCE CAUTION:
CC       Sequence=AAD20061.1; Type=Erroneous initiation; Note=Extended N-terminus.; Evidence={ECO:0000305};
CC       Sequence=AAH39396.2; Type=Erroneous initiation; Note=Truncated N-terminus.; Evidence={ECO:0000305};
CC   ---------------------------------------------------------------------------
CC   Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms
CC   Distributed under the Creative Commons Attribution (CC BY 4.0) License
CC   ---------------------------------------------------------------------------
DR   EMBL; AY325118; AAP92410.1; -; mRNA.
DR   EMBL; AF131851; AAD20061.1; ALT_INIT; mRNA.
DR   EMBL; AY358847; AAQ89206.1; -; mRNA.
DR   EMBL; AK123443; BAG53905.1; -; mRNA.
DR   EMBL; AK125256; BAG54175.1; -; mRNA.
DR   EMBL; AL096843; -; NOT_ANNOTATED_CDS; Genomic_DNA.
DR   EMBL; AL096853; -; NOT_ANNOTATED_CDS; Genomic_DNA.
DR   EMBL; Z83837; -; NOT_ANNOTATED_CDS; Genomic_DNA.
DR   EMBL; Z84468; -; NOT_ANNOTATED_CDS; Genomic_DNA.
DR   EMBL; BC039396; AAH39396.2; ALT_INIT; mRNA.
DR   CCDS; CCDS46728.1; -. [Q7Z5A7-1]
DR   CCDS; CCDS46729.1; -. [Q7Z5A7-2]
DR   RefSeq; NP_001076436.1; NM_001082967.2. [Q7Z5A7-1]
DR   RefSeq; NP_056196.2; NM_015381.6. [Q7Z5A7-2]
DR   AlphaFoldDB; Q7Z5A7; -.
DR   BioGRID; 117345; 23.
DR   IntAct; Q7Z5A7; 1.
DR   MINT; Q7Z5A7; -.
DR   STRING; 9606.ENSP00000383933; -.
DR   GlyCosmos; Q7Z5A7; 1 site, No reported glycans.
DR   GlyGen; Q7Z5A7; 1 site.
DR   iPTMnet; Q7Z5A7; -.
DR   PhosphoSitePlus; Q7Z5A7; -.
DR   BioMuta; FAM19A5; -.
DR   DMDM; 205371754; -.
DR   MassIVE; Q7Z5A7; -.
DR   PaxDb; 9606-ENSP00000383933; -.
DR   PeptideAtlas; Q7Z5A7; -.
DR   ProteomicsDB; 69275; -. [Q7Z5A7-1]
DR   ProteomicsDB; 69276; -. [Q7Z5A7-2]
DR   ProteomicsDB; 69277; -. [Q7Z5A7-3]
DR   Antibodypedia; 67212; 22 antibodies from 13 providers.
DR   DNASU; 25817; -.
DR   Ensembl; ENST00000358295.9; ENSP00000351043.5; ENSG00000219438.9. [Q7Z5A7-2]
DR   Ensembl; ENST00000402357.6; ENSP00000383933.2; ENSG00000219438.9. [Q7Z5A7-1]
DR   Ensembl; ENST00000406880.1; ENSP00000385603.1; ENSG00000219438.9. [Q7Z5A7-3]
DR   GeneID; 25817; -.
DR   KEGG; hsa:25817; -.
DR   MANE-Select; ENST00000402357.6; ENSP00000383933.2; NM_001082967.3; NP_001076436.1.
DR   UCSC; uc003bim.6; human. [Q7Z5A7-1]
DR   AGR; HGNC:21592; -.
DR   CTD; 25817; -.
DR   DisGeNET; 25817; -.
DR   GeneCards; TAFA5; -.
DR   HGNC; HGNC:21592; TAFA5.
DR   HPA; ENSG00000219438; Tissue enriched (brain).
DR   MIM; 617499; gene.
DR   neXtProt; NX_Q7Z5A7; -.
DR   OpenTargets; ENSG00000219438; -.
DR   PharmGKB; PA134887312; -.
DR   VEuPathDB; HostDB:ENSG00000219438; -.
DR   eggNOG; ENOG502RZT2; Eukaryota.
DR   GeneTree; ENSGT00940000160682; -.
DR   HOGENOM; CLU_126078_5_0_1; -.
DR   InParanoid; Q7Z5A7; -.
DR   OMA; SGWRCAQ; -.
DR   OrthoDB; 5348365at2759; -.
DR   PhylomeDB; Q7Z5A7; -.
DR   TreeFam; TF331749; -.
DR   PathwayCommons; Q7Z5A7; -.
DR   SignaLink; Q7Z5A7; -.
DR   BioGRID-ORCS; 25817; 13 hits in 1144 CRISPR screens.
DR   ChiTaRS; FAM19A5; human.
DR   GeneWiki; FAM19A5; -.
DR   GenomeRNAi; 25817; -.
DR   Pharos; Q7Z5A7; Tdark.
DR   PRO; PR:Q7Z5A7; -.
DR   Proteomes; UP000005640; Chromosome 22.
DR   RNAct; Q7Z5A7; Protein.
DR   Bgee; ENSG00000219438; Expressed in cerebellar vermis and 153 other cell types or tissues.
DR   ExpressionAtlas; Q7Z5A7; baseline and differential.
DR   GO; GO:0005737; C:cytoplasm; IEA:Ensembl.
DR   GO; GO:0005615; C:extracellular space; IDA:MGI.
DR   GO; GO:0005125; F:cytokine activity; IEA:UniProtKB-KW.
DR   GO; GO:0001664; F:G protein-coupled receptor binding; IPI:MGI.
DR   GO; GO:0048018; F:receptor ligand activity; IPI:MGI.
DR   GO; GO:0007186; P:G protein-coupled receptor signaling pathway; IDA:MGI.
DR   GO; GO:1904753; P:negative regulation of vascular associated smooth muscle cell migration; IEA:Ensembl.
DR   GO; GO:1904706; P:negative regulation of vascular associated smooth muscle cell proliferation; IDA:MGI.
DR   GO; GO:0061044; P:negative regulation of vascular wound healing; IEA:Ensembl.
DR   InterPro; IPR020350; Chemokine-like_TAFA.
DR   InterPro; IPR040329; TAFA-5.
DR   PANTHER; PTHR31878:SF0; CHEMOKINE-LIKE PROTEIN TAFA-5; 1.
DR   PANTHER; PTHR31878; CHEMOKINE-LIKE PROTEIN TAFA-5-RELATED; 1.
DR   Pfam; PF12020; TAFA; 1.
DR   Genevisible; Q7Z5A7; HS.
PE   1: Evidence at protein level;
KW   Alternative splicing; Cytokine; Direct protein sequencing; Glycoprotein;
KW   Reference proteome; Secreted; Signal.
FT   SIGNAL          1..43
FT                   /evidence="ECO:0000269|PubMed:29453251"
FT   CHAIN           44..132
FT                   /note="Chemokine-like protein TAFA-5"
FT                   /evidence="ECO:0000305|PubMed:29453251"
FT                   /id="PRO_0000042732"
FT   CARBOHYD        113
FT                   /note="N-linked (GlcNAc...) asparagine"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00498"
FT   VAR_SEQ         1..87
FT                   /note="MAPSPRTGSRQDATALPSMSSTFWAFMILASLLIAYCSQLAAGTCEIVTLDR
FT                   DSSQPRRTIARQTARCACRKGQIAGTTRARPACVD -> MYHHREWP (in isoform
FT                   3)"
FT                   /evidence="ECO:0000305"
FT                   /id="VSP_035191"
FT   VAR_SEQ         1..38
FT                   /note="MAPSPRTGSRQDATALPSMSSTFWAFMILASLLIAYCS -> MQLLKALWAL
FT                   AGAALCCFLVLVIHAQFLKEG (in isoform 2)"
FT                   /evidence="ECO:0000303|PubMed:12975309,
FT                   ECO:0000303|PubMed:14702039, ECO:0000303|PubMed:15028294"
FT                   /id="VSP_016068"
FT   CONFLICT        Q7Z5A7-2:11..23
FT                   /note="AGAALCCFLVLVI -> V (in Ref. 3; AAQ89206)"
FT                   /evidence="ECO:0000305"
SQ   SEQUENCE   132 AA;  14301 MW;  16BFE9F574FBB066 CRC64;
     MAPSPRTGSR QDATALPSMS STFWAFMILA SLLIAYCSQL AAGTCEIVTL DRDSSQPRRT
     IARQTARCAC RKGQIAGTTR ARPACVDARI IKTKQWCDML PCLEGEGCDL LINRSGWTCT
     QPGGRIKTTT VS
//