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Protein

BRCA1-associated protein

Gene

BRAP

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Negatively regulates MAP kinase activation by limiting the formation of Raf/MEK complexes probably by inactivation of the KSR1 scaffold protein. Also acts as a Ras responsive E3 ubiquitin ligase that, on activation of Ras, is modified by auto-polyubiquitination resulting in the release of inhibition of Raf/MEK complex formation. May also act as a cytoplasmic retention protein with a role in regulating nuclear transport.1 Publication1 Publication

Pathwayi: protein ubiquitination

This protein is involved in the pathway protein ubiquitination, which is part of Protein modification.
View all proteins of this organism that are known to be involved in the pathway protein ubiquitination and in Protein modification.

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Zinc fingeri264 – 30441RING-typePROSITE-ProRule annotationCuratedAdd
BLAST
Zinc fingeri315 – 37662UBP-typePROSITE-ProRule annotationAdd
BLAST

GO - Molecular functioni

  • identical protein binding Source: IntAct
  • ligase activity Source: UniProtKB-KW
  • nuclear localization sequence binding Source: MGI
  • nucleotide binding Source: InterPro
  • ubiquitin binding Source: GO_Central
  • ubiquitin protein ligase activity Source: MGI
  • ubiquitin-protein transferase activity Source: UniProtKB
  • zinc ion binding Source: InterPro

GO - Biological processi

  • MAPK cascade Source: MGI
  • negative regulation of signal transduction Source: UniProtKB
  • protein ubiquitination Source: FlyBase
  • Ras protein signal transduction Source: MGI
Complete GO annotation...

Keywords - Molecular functioni

Ligase

Keywords - Biological processi

Ubl conjugation pathway

Keywords - Ligandi

Metal-binding, Zinc

Enzyme and pathway databases

ReactomeiR-HSA-5673000. RAF activation.
R-HSA-5675221. Negative regulation of MAPK pathway.
UniPathwayiUPA00143.

Names & Taxonomyi

Protein namesi
Recommended name:
BRCA1-associated protein (EC:6.3.2.-)
Alternative name(s):
BRAP2
Impedes mitogenic signal propagation
Short name:
IMP
RING finger protein 52
Renal carcinoma antigen NY-REN-63
Gene namesi
Name:BRAPImported
Synonyms:RNF52
OrganismiHomo sapiens (Human)Imported
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
Proteomesi
  • UP000005640 Componenti: Chromosome 12

Organism-specific databases

HGNCiHGNC:1099. BRAP.

Subcellular locationi

GO - Cellular componenti

  • cytoplasm Source: UniProtKB
  • cytosol Source: Reactome
  • nuclear membrane Source: HPA
  • ubiquitin ligase complex Source: MGI
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Mutagenesisi264 – 2641C → A: Loss of E3 ubiquitin-protein ligase activity. 1 Publication

Organism-specific databases

PharmGKBiPA25410.

Polymorphism and mutation databases

BioMutaiBRAP.
DMDMi296434410.

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 592592BRCA1-associated proteinPRO_0000055825Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei52 – 521PhosphoserineCombined sources
Modified residuei97 – 971PhosphoserineCombined sources
Modified residuei117 – 1171PhosphoserineCombined sources
Modified residuei119 – 1191PhosphoserineCombined sources

Keywords - PTMi

Phosphoprotein

Proteomic databases

EPDiQ7Z569.
MaxQBiQ7Z569.
PaxDbiQ7Z569.
PeptideAtlasiQ7Z569.
PRIDEiQ7Z569.

PTM databases

iPTMnetiQ7Z569.
PhosphoSiteiQ7Z569.

Expressioni

Tissue specificityi

Expressed in breast epithelial cell lines.1 Publication

Gene expression databases

BgeeiENSG00000089234.
CleanExiHS_BRAP.
ExpressionAtlasiQ7Z569. baseline and differential.
GenevisibleiQ7Z569. HS.

Organism-specific databases

HPAiHPA040357.
HPA058820.

Interactioni

Subunit structurei

Interacts with the nuclear localization signal of BRCA1 and with the N-terminal of KSR1. The C-terminal portion of BCRA1 interacts with DDB1.3 Publications

Binary interactionsi

WithEntry#Exp.IntActNotes
itself2EBI-349900,EBI-349900
AKAP3O759692EBI-349900,EBI-9033101
BRCA1P383983EBI-349900,EBI-349905
HRASP011123EBI-349900,EBI-350145

GO - Molecular functioni

  • identical protein binding Source: IntAct
  • ubiquitin binding Source: GO_Central

Protein-protein interaction databases

BioGridi113912. 56 interactions.
IntActiQ7Z569. 42 interactions.
MINTiMINT-1532927.
STRINGi9606.ENSP00000403524.

Structurei

3D structure databases

ProteinModelPortaliQ7Z569.
SMRiQ7Z569. Positions 256-308, 317-375.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Coiled coil

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Coiled coili429 – 537109Sequence analysisAdd
BLAST

Sequence similaritiesi

Contains 1 RING-type zinc finger.PROSITE-ProRule annotationCurated
Contains 1 UBP-type zinc finger.PROSITE-ProRule annotation

Zinc finger

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Zinc fingeri264 – 30441RING-typePROSITE-ProRule annotationCuratedAdd
BLAST
Zinc fingeri315 – 37662UBP-typePROSITE-ProRule annotationAdd
BLAST

Keywords - Domaini

Coiled coil, Zinc-finger

Phylogenomic databases

eggNOGiKOG0804. Eukaryota.
ENOG410XSS2. LUCA.
GeneTreeiENSGT00500000044909.
HOGENOMiHOG000190616.
HOVERGENiHBG050729.
InParanoidiQ7Z569.
KOiK10632.
OMAiCLQRWED.
OrthoDBiEOG091G07BQ.
PhylomeDBiQ7Z569.
TreeFamiTF313622.

Family and domain databases

Gene3Di3.30.40.10. 2 hits.
3.30.70.330. 1 hit.
InterProiIPR011422. BRAP2.
IPR012677. Nucleotide-bd_a/b_plait.
IPR001841. Znf_RING.
IPR013083. Znf_RING/FYVE/PHD.
IPR001607. Znf_UBP.
[Graphical view]
PfamiPF07576. BRAP2. 1 hit.
PF13639. zf-RING_2. 1 hit.
PF02148. zf-UBP. 1 hit.
[Graphical view]
ProDomiPD017029. BRAP2. 1 hit.
[Graphical view] [Entries sharing at least one domain]
SMARTiSM00184. RING. 1 hit.
SM00290. ZnF_UBP. 1 hit.
[Graphical view]
SUPFAMiSSF54928. SSF54928. 1 hit.
PROSITEiPS50089. ZF_RING_2. 1 hit.
PS50271. ZF_UBP. 1 hit.
[Graphical view]

Sequences (2)i

Sequence statusi: Complete.

This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 1 (identifier: Q7Z569-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MSVSLVVIRL ELAEHSPVPA GFGFSAAAGE MSDEEIKKTT LASAVACLEG
60 70 80 90 100
KSPGEKVAII HQHLGRREMT DVIIETMKSN PDELKTTVEE RKSSEASPTA
110 120 130 140 150
QRSKDHSKEC INAAPDSPSK QLPDQISFFS GNPSVEIVHG IMHLYKTNKM
160 170 180 190 200
TSLKEDVRRS AMLCILTVPA AMTSHDLMKF VAPFNEVIEQ MKIIRDSTPN
210 220 230 240 250
QYMVLIKFRA QADADSFYMT CNGRQFNSIE DDVCQLVYVE RAEVLKSEDG
260 270 280 290 300
ASLPVMDLTE LPKCTVCLER MDESVNGILT TLCNHSFHSQ CLQRWDDTTC
310 320 330 340 350
PVCRYCQTPE PVEENKCFEC GVQENLWICL ICGHIGCGRY VSRHAYKHFE
360 370 380 390 400
ETQHTYAMQL TNHRVWDYAG DNYVHRLVAS KTDGKIVQYE CEGDTCQEEK
410 420 430 440 450
IDALQLEYSY LLTSQLESQR IYWENKIVRI EKDTAEEINN MKTKFKETIE
460 470 480 490 500
KCDNLEHKLN DLLKEKQSVE RKCTQLNTKV AKLTNELKEE QEMNKCLRAN
510 520 530 540 550
QVLLQNKLKE EERVLKETCD QKDLQITEIQ EQLRDVMFYL ETQQKINHLP
560 570 580 590
AETRQEIQEG QINIAMASAS SPASSGGSGK LPSRKGRSKR GK
Length:592
Mass (Da):67,305
Last modified:May 18, 2010 - v2
Checksum:i70CFEAB560D1952C
GO
Isoform 2 (identifier: Q7Z569-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     1-141: Missing.
     212-249: Missing.

Note: No experimental confirmation available.
Show »
Length:413
Mass (Da):47,853
Checksum:iF54A77311160F312
GO

Sequence cautioni

The sequence AAC24200 differs from that shown. Reason: Frameshift at position 556. Curated

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti186 – 1861E → D in AAC24200 (PubMed:9497340).Curated
Sequence conflicti186 – 1861E → D in AAP93638 (PubMed:14724641).Curated
Sequence conflicti356 – 3561Y → H in AAB88538 (PubMed:10777491).Curated

Alternative sequence

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Alternative sequencei1 – 141141Missing in isoform 2. 1 PublicationVSP_055881Add
BLAST
Alternative sequencei212 – 24938Missing in isoform 2. 1 PublicationVSP_055882Add
BLAST

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF035620 mRNA. Translation: AAC24200.1. Frameshift.
AY332222 mRNA. Translation: AAP93638.1.
AK299326 mRNA. Translation: BAG61333.1.
AC002996 Genomic DNA. No translation available.
AC137055 Genomic DNA. No translation available.
CH471054 Genomic DNA. Translation: EAW97972.1.
BC136698 mRNA. Translation: AAI36699.1.
BC136699 mRNA. Translation: AAI36700.1.
AF035950 mRNA. Translation: AAB88538.1.
CCDSiCCDS9154.1. [Q7Z569-1]
RefSeqiNP_006759.3. NM_006768.4. [Q7Z569-1]
UniGeneiHs.530940.

Genome annotation databases

EnsembliENST00000419234; ENSP00000403524; ENSG00000089234. [Q7Z569-1]
GeneIDi8315.
KEGGihsa:8315.
UCSCiuc001tsn.4. human. [Q7Z569-1]

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF035620 mRNA. Translation: AAC24200.1. Frameshift.
AY332222 mRNA. Translation: AAP93638.1.
AK299326 mRNA. Translation: BAG61333.1.
AC002996 Genomic DNA. No translation available.
AC137055 Genomic DNA. No translation available.
CH471054 Genomic DNA. Translation: EAW97972.1.
BC136698 mRNA. Translation: AAI36699.1.
BC136699 mRNA. Translation: AAI36700.1.
AF035950 mRNA. Translation: AAB88538.1.
CCDSiCCDS9154.1. [Q7Z569-1]
RefSeqiNP_006759.3. NM_006768.4. [Q7Z569-1]
UniGeneiHs.530940.

3D structure databases

ProteinModelPortaliQ7Z569.
SMRiQ7Z569. Positions 256-308, 317-375.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi113912. 56 interactions.
IntActiQ7Z569. 42 interactions.
MINTiMINT-1532927.
STRINGi9606.ENSP00000403524.

PTM databases

iPTMnetiQ7Z569.
PhosphoSiteiQ7Z569.

Polymorphism and mutation databases

BioMutaiBRAP.
DMDMi296434410.

Proteomic databases

EPDiQ7Z569.
MaxQBiQ7Z569.
PaxDbiQ7Z569.
PeptideAtlasiQ7Z569.
PRIDEiQ7Z569.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENST00000419234; ENSP00000403524; ENSG00000089234. [Q7Z569-1]
GeneIDi8315.
KEGGihsa:8315.
UCSCiuc001tsn.4. human. [Q7Z569-1]

Organism-specific databases

CTDi8315.
GeneCardsiBRAP.
H-InvDBHIX0036684.
HGNCiHGNC:1099. BRAP.
HPAiHPA040357.
HPA058820.
MIMi604986. gene.
neXtProtiNX_Q7Z569.
PharmGKBiPA25410.
GenAtlasiSearch...

Phylogenomic databases

eggNOGiKOG0804. Eukaryota.
ENOG410XSS2. LUCA.
GeneTreeiENSGT00500000044909.
HOGENOMiHOG000190616.
HOVERGENiHBG050729.
InParanoidiQ7Z569.
KOiK10632.
OMAiCLQRWED.
OrthoDBiEOG091G07BQ.
PhylomeDBiQ7Z569.
TreeFamiTF313622.

Enzyme and pathway databases

UniPathwayiUPA00143.
ReactomeiR-HSA-5673000. RAF activation.
R-HSA-5675221. Negative regulation of MAPK pathway.

Miscellaneous databases

ChiTaRSiBRAP. human.
GenomeRNAii8315.
PROiQ7Z569.
SOURCEiSearch...

Gene expression databases

BgeeiENSG00000089234.
CleanExiHS_BRAP.
ExpressionAtlasiQ7Z569. baseline and differential.
GenevisibleiQ7Z569. HS.

Family and domain databases

Gene3Di3.30.40.10. 2 hits.
3.30.70.330. 1 hit.
InterProiIPR011422. BRAP2.
IPR012677. Nucleotide-bd_a/b_plait.
IPR001841. Znf_RING.
IPR013083. Znf_RING/FYVE/PHD.
IPR001607. Znf_UBP.
[Graphical view]
PfamiPF07576. BRAP2. 1 hit.
PF13639. zf-RING_2. 1 hit.
PF02148. zf-UBP. 1 hit.
[Graphical view]
ProDomiPD017029. BRAP2. 1 hit.
[Graphical view] [Entries sharing at least one domain]
SMARTiSM00184. RING. 1 hit.
SM00290. ZnF_UBP. 1 hit.
[Graphical view]
SUPFAMiSSF54928. SSF54928. 1 hit.
PROSITEiPS50089. ZF_RING_2. 1 hit.
PS50271. ZF_UBP. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiBRAP_HUMAN
AccessioniPrimary (citable) accession number: Q7Z569
Secondary accession number(s): B4DRM1
, B9EGS8, O43238, O75341
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 19, 2004
Last sequence update: May 18, 2010
Last modified: September 7, 2016
This is version 131 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Human chromosome 12
    Human chromosome 12: entries, gene names and cross-references to MIM
  2. MIM cross-references
    Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
  3. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  4. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.