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Q7Z4I7 (LIMS2_HUMAN) Reviewed, UniProtKB/Swiss-Prot

Last modified July 9, 2014. Version 92. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (4) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Alt products·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
LIM and senescent cell antigen-like-containing domain protein 2
Alternative name(s):
LIM-like protein 2
Particularly interesting new Cys-His protein 2
Short name=PINCH-2
Gene names
Name:LIMS2
Synonyms:PINCH2
OrganismHomo sapiens (Human) [Reference proteome]
Taxonomic identifier9606 [NCBI]
Taxonomic lineageEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo

Protein attributes

Sequence length341 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Adapter protein in a cytoplasmic complex linking beta-integrins to the actin cytoskeleton, bridges the complex to cell surface receptor tyrosine kinases and growth factor receptors. Plays a role in modulating cell spreading and migration. Ref.1

Subunit structure

Interacts with TGFB1I1 By similarity. Interacts with integrin-linked protein kinase 1 (ILK) via the first LIM domain, and in competition with LIMS1. Part of the heterotrimeric IPP complex composed of integrin-linked kinase (ILK), LIMS1 or LIMS2, and PARVA. Ref.1 Ref.10

Subcellular location

Nucleus Potential. Cell junctionfocal adhesion. Cell membrane; Peripheral membrane protein; Cytoplasmic side Ref.1.

Sequence similarities

Contains 5 LIM zinc-binding domains.

Sequence caution

The sequence AAM77350.1 differs from that shown. Reason: Frameshift at position 331.

Alternative products

This entry describes 5 isoforms produced by alternative splicing. [Align] [Select]
Isoform 1 (identifier: Q7Z4I7-1)

Also known as: LIM-like protein 2B;

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.
Isoform 2 (identifier: Q7Z4I7-2)

Also known as: LIM-like protein 2A;

The sequence of this isoform differs from the canonical sequence as follows:
     1-4: MTGS → MAARLGALAASGLYRRRQHRQSPPPATG
Isoform 3 (identifier: Q7Z4I7-3)

Also known as: LIM-like protein 2C;

The sequence of this isoform differs from the canonical sequence as follows:
     1-5: Missing.
Isoform 4 (identifier: Q7Z4I7-4)

The sequence of this isoform differs from the canonical sequence as follows:
     1-152: Missing.
Isoform 5 (identifier: Q7Z4I7-5)

The sequence of this isoform differs from the canonical sequence as follows:
     3-3: G → GRKRKWGETGTGSGAAPAAALRW
Note: No experimental confirmation available.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 341341LIM and senescent cell antigen-like-containing domain protein 2
PRO_0000266011

Regions

Domain13 – 7462LIM zinc-binding 1
Domain76 – 13358LIM zinc-binding 2
Domain138 – 19558LIM zinc-binding 3
Domain196 – 25560LIM zinc-binding 4
Domain256 – 31560LIM zinc-binding 5

Amino acid modifications

Modified residue3271Phosphothreonine Ref.8
Modified residue3281Phosphoserine Ref.8

Natural variations

Alternative sequence1 – 152152Missing in isoform 4.
VSP_045539
Alternative sequence1 – 55Missing in isoform 3.
VSP_021916
Alternative sequence1 – 44MTGS → MAARLGALAASGLYRRRQHR QSPPPATG in isoform 2.
VSP_021917
Alternative sequence31G → GRKRKWGETGTGSGAAPAAA LRW in isoform 5.
VSP_046078

Experimental info

Sequence conflict1111D → G in BAG60013. Ref.5
Sequence conflict2941N → D in AAM97589. Ref.1
Sequence conflict2941N → D in BAB14047. Ref.6

Secondary structure

................... 341
Helix Strand Turn

Details...

Sequences

Sequence LengthMass (Da)Tools
Isoform 1 (LIM-like protein 2B) [UniParc].

Last modified October 1, 2003. Version 1.
Checksum: EEA8D74C309D9C8A

FASTA34138,916
        10         20         30         40         50         60 
MTGSNMSDAL ANAVCQRCQA RFSPAERIVN SNGELYHEHC FVCAQCFRPF PEGLFYEFEG 

        70         80         90        100        110        120 
RKYCEHDFQM LFAPCCGSCG EFIIGRVIKA MNNNWHPGCF RCELCDVELA DLGFVKNAGR 

       130        140        150        160        170        180 
HLCRPCHNRE KAKGLGKYIC QRCHLVIDEQ PLMFRSDAYH PDHFNCTHCG KELTAEAREL 

       190        200        210        220        230        240 
KGELYCLPCH DKMGVPICGA CRRPIEGRVV NALGKQWHVE HFVCAKCEKP FLGHRHYEKK 

       250        260        270        280        290        300 
GLAYCETHYN QLFGDVCYNC SHVIEGDVVS ALNKAWCVSC FSCSTCNSKL TLKNKFVEFD 

       310        320        330        340 
MKPVCKRCYE KFPLELKKRL KKLSELTSRK AQPKATDLNS A 

« Hide

Isoform 2 (LIM-like protein 2A) [UniParc].

Checksum: 3B8E1C98AFB2B698
Show »

FASTA36541,512
Isoform 3 (LIM-like protein 2C) [UniParc].

Checksum: 27F7EA97B557804D
Show »

FASTA33638,426
Isoform 4 [UniParc].

Checksum: 896862131C240B5B
Show »

FASTA18921,615
Isoform 5 [UniParc].

Checksum: D0F6B8B3618F20FF
Show »

FASTA36341,226

References

« Hide 'large scale' references
[1]"Characterization of PINCH-2, a new focal adhesion protein that regulates the PINCH-1-ILK interaction, cell spreading, and migration."
Zhang Y., Chen K., Guo L., Wu C.
J. Biol. Chem. 277:38328-38338(2002) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1), FUNCTION, SUBCELLULAR LOCATION, INTERACTION WITH ILK.
[2]"Cloning and characterization a novel human gene LIMS2."
Zeng W., Zhu Y., Jiao W., Yuan W., Wu X.
Submitted (JUN-2002) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 3).
[3]Ding P., Han W., Cheng Y., Qiu X., Song Q., Zhang Y., Ma D.
Submitted (JUL-2002) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORMS 1; 2; 3 AND 4).
Tissue: Brain and Lung.
[4]"Full-length cDNA libraries and normalization."
Li W.B., Gruber C., Jessee J., Polayes D.
Submitted (APR-2003) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 4).
Tissue: Placenta.
[5]"Complete sequencing and characterization of 21,243 full-length human cDNAs."
Ota T., Suzuki Y., Nishikawa T., Otsuki T., Sugiyama T., Irie R., Wakamatsu A., Hayashi K., Sato H., Nagai K., Kimura K., Makita H., Sekine M., Obayashi M., Nishi T., Shibahara T., Tanaka T., Ishii S. expand/collapse author list , Yamamoto J., Saito K., Kawai Y., Isono Y., Nakamura Y., Nagahari K., Murakami K., Yasuda T., Iwayanagi T., Wagatsuma M., Shiratori A., Sudo H., Hosoiri T., Kaku Y., Kodaira H., Kondo H., Sugawara M., Takahashi M., Kanda K., Yokoi T., Furuya T., Kikkawa E., Omura Y., Abe K., Kamihara K., Katsuta N., Sato K., Tanikawa M., Yamazaki M., Ninomiya K., Ishibashi T., Yamashita H., Murakawa K., Fujimori K., Tanai H., Kimata M., Watanabe M., Hiraoka S., Chiba Y., Ishida S., Ono Y., Takiguchi S., Watanabe S., Yosida M., Hotuta T., Kusano J., Kanehori K., Takahashi-Fujii A., Hara H., Tanase T.-O., Nomura Y., Togiya S., Komai F., Hara R., Takeuchi K., Arita M., Imose N., Musashino K., Yuuki H., Oshima A., Sasaki N., Aotsuka S., Yoshikawa Y., Matsunawa H., Ichihara T., Shiohata N., Sano S., Moriya S., Momiyama H., Satoh N., Takami S., Terashima Y., Suzuki O., Nakagawa S., Senoh A., Mizoguchi H., Goto Y., Shimizu F., Wakebe H., Hishigaki H., Watanabe T., Sugiyama A., Takemoto M., Kawakami B., Yamazaki M., Watanabe K., Kumagai A., Itakura S., Fukuzumi Y., Fujimori Y., Komiyama M., Tashiro H., Tanigami A., Fujiwara T., Ono T., Yamada K., Fujii Y., Ozaki K., Hirao M., Ohmori Y., Kawabata A., Hikiji T., Kobatake N., Inagaki H., Ikema Y., Okamoto S., Okitani R., Kawakami T., Noguchi S., Itoh T., Shigeta K., Senba T., Matsumura K., Nakajima Y., Mizuno T., Morinaga M., Sasaki M., Togashi T., Oyama M., Hata H., Watanabe M., Komatsu T., Mizushima-Sugano J., Satoh T., Shirai Y., Takahashi Y., Nakagawa K., Okumura K., Nagase T., Nomura N., Kikuchi H., Masuho Y., Yamashita R., Nakai K., Yada T., Nakamura Y., Ohara O., Isogai T., Sugano S.
Nat. Genet. 36:40-45(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORMS 1 AND 5).
Tissue: Brain and Mammary gland.
[6]"Generation and annotation of the DNA sequences of human chromosomes 2 and 4."
Hillier L.W., Graves T.A., Fulton R.S., Fulton L.A., Pepin K.H., Minx P., Wagner-McPherson C., Layman D., Wylie K., Sekhon M., Becker M.C., Fewell G.A., Delehaunty K.D., Miner T.L., Nash W.E., Kremitzki C., Oddy L., Du H. expand/collapse author list , Sun H., Bradshaw-Cordum H., Ali J., Carter J., Cordes M., Harris A., Isak A., van Brunt A., Nguyen C., Du F., Courtney L., Kalicki J., Ozersky P., Abbott S., Armstrong J., Belter E.A., Caruso L., Cedroni M., Cotton M., Davidson T., Desai A., Elliott G., Erb T., Fronick C., Gaige T., Haakenson W., Haglund K., Holmes A., Harkins R., Kim K., Kruchowski S.S., Strong C.M., Grewal N., Goyea E., Hou S., Levy A., Martinka S., Mead K., McLellan M.D., Meyer R., Randall-Maher J., Tomlinson C., Dauphin-Kohlberg S., Kozlowicz-Reilly A., Shah N., Swearengen-Shahid S., Snider J., Strong J.T., Thompson J., Yoakum M., Leonard S., Pearman C., Trani L., Radionenko M., Waligorski J.E., Wang C., Rock S.M., Tin-Wollam A.-M., Maupin R., Latreille P., Wendl M.C., Yang S.-P., Pohl C., Wallis J.W., Spieth J., Bieri T.A., Berkowicz N., Nelson J.O., Osborne J., Ding L., Meyer R., Sabo A., Shotland Y., Sinha P., Wohldmann P.E., Cook L.L., Hickenbotham M.T., Eldred J., Williams D., Jones T.A., She X., Ciccarelli F.D., Izaurralde E., Taylor J., Schmutz J., Myers R.M., Cox D.R., Huang X., McPherson J.D., Mardis E.R., Clifton S.W., Warren W.C., Chinwalla A.T., Eddy S.R., Marra M.A., Ovcharenko I., Furey T.S., Miller W., Eichler E.E., Bork P., Suyama M., Torrents D., Waterston R.H., Wilson R.K.
Nature 434:724-731(2005) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[7]"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
The MGC Project Team
Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
Tissue: PNS.
[8]"A quantitative atlas of mitotic phosphorylation."
Dephoure N., Zhou C., Villen J., Beausoleil S.A., Bakalarski C.E., Elledge S.J., Gygi S.P.
Proc. Natl. Acad. Sci. U.S.A. 105:10762-10767(2008) [PubMed] [Europe PMC] [Abstract]
Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT THR-327 AND SER-328, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
Tissue: Cervix carcinoma.
[9]"N-terminal acetylome analyses and functional insights of the N-terminal acetyltransferase NatB."
Van Damme P., Lasa M., Polevoda B., Gazquez C., Elosegui-Artola A., Kim D.S., De Juan-Pardo E., Demeyer K., Hole K., Larrea E., Timmerman E., Prieto J., Arnesen T., Sherman F., Gevaert K., Aldabe R.
Proc. Natl. Acad. Sci. U.S.A. 109:12449-12454(2012) [PubMed] [Europe PMC] [Abstract]
Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
[10]"Structural basis of competition between PINCH1 and PINCH2 for binding to the ankyrin repeat domain of integrin-linked kinase."
Chiswell B.P., Stiegler A.L., Razinia Z., Nalibotski E., Boggon T.J., Calderwood D.A.
J. Struct. Biol. 170:157-163(2010) [PubMed] [Europe PMC] [Abstract]
Cited for: X-RAY CRYSTALLOGRAPHY (1.9 ANGSTROMS) OF 11-73 IN COMPLEX WITH ILK, SUBUNIT.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AF484961 mRNA. Translation: AAM97589.1.
AF520987 mRNA. Translation: AAM77350.1. Frameshift.
AF527764 mRNA. Translation: AAQ09011.1.
AF527765 mRNA. Translation: AAQ09012.1.
AF527766 mRNA. Translation: AAQ09013.1.
AF527770 mRNA. Translation: AAQ09017.1.
AK022470 mRNA. Translation: BAB14047.1.
AK297645 mRNA. Translation: BAG60013.1.
BX458594 mRNA. No translation available.
AC010976 Genomic DNA. No translation available.
AC074114 Genomic DNA. No translation available.
BC065816 mRNA. Translation: AAH65816.1.
CCDSCCDS2147.1. [Q7Z4I7-2]
CCDS54394.1. [Q7Z4I7-3]
CCDS54395.1. [Q7Z4I7-1]
CCDS54396.1. [Q7Z4I7-5]
CCDS58725.1. [Q7Z4I7-4]
RefSeqNP_001129509.2. NM_001136037.2. [Q7Z4I7-5]
NP_001154875.1. NM_001161403.1. [Q7Z4I7-1]
NP_001154876.1. NM_001161404.1. [Q7Z4I7-3]
NP_001243471.1. NM_001256542.1. [Q7Z4I7-4]
NP_060450.2. NM_017980.4. [Q7Z4I7-2]
XP_005263766.1. XM_005263709.1. [Q7Z4I7-3]
UniGeneHs.469881.

3D structure databases

PDBe
RCSB-PDB
PDBj
EntryMethodResolution (Å)ChainPositionsPDBsum
3IXEX-ray1.90B11-73[»]
ProteinModelPortalQ7Z4I7.
SMRQ7Z4I7. Positions 11-310.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

BioGrid120808. 3 interactions.
IntActQ7Z4I7. 3 interactions.
MINTMINT-1194006.
STRING9606.ENSP00000326888.

PTM databases

PhosphoSiteQ7Z4I7.

Polymorphism databases

DMDM74750091.

Proteomic databases

MaxQBQ7Z4I7.
PaxDbQ7Z4I7.
PRIDEQ7Z4I7.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblENST00000324938; ENSP00000326888; ENSG00000072163. [Q7Z4I7-2]
ENST00000355119; ENSP00000347240; ENSG00000072163. [Q7Z4I7-1]
ENST00000409254; ENSP00000386907; ENSG00000072163. [Q7Z4I7-4]
ENST00000409286; ENSP00000386252; ENSG00000072163. [Q7Z4I7-4]
ENST00000409455; ENSP00000386383; ENSG00000072163. [Q7Z4I7-3]
ENST00000409754; ENSP00000386345; ENSG00000072163. [Q7Z4I7-4]
ENST00000409808; ENSP00000386637; ENSG00000072163. [Q7Z4I7-3]
ENST00000410011; ENSP00000387002; ENSG00000072163. [Q7Z4I7-3]
ENST00000410038; ENSP00000386570; ENSG00000072163. [Q7Z4I7-4]
ENST00000545738; ENSP00000443794; ENSG00000072163. [Q7Z4I7-5]
GeneID55679.
KEGGhsa:55679.
UCSCuc002tov.3. human. [Q7Z4I7-1]
uc002tox.3. human. [Q7Z4I7-2]
uc010yzm.2. human.

Organism-specific databases

CTD55679.
GeneCardsGC02M128395.
HGNCHGNC:16084. LIMS2.
HPAHPA058340.
MIM607908. gene.
neXtProtNX_Q7Z4I7.
PharmGKBPA30390.
GenAtlasSearch...

Phylogenomic databases

eggNOGNOG263350.
HOVERGENHBG000053.
OMAECFRCEL.
PhylomeDBQ7Z4I7.
TreeFamTF314113.

Enzyme and pathway databases

ReactomeREACT_111155. Cell-Cell communication.

Gene expression databases

ArrayExpressQ7Z4I7.
BgeeQ7Z4I7.
CleanExHS_LIMS2.
GenevestigatorQ7Z4I7.

Family and domain databases

Gene3D2.10.110.10. 5 hits.
InterProIPR017351. PINCH.
IPR001781. Znf_LIM.
[Graphical view]
PANTHERPTHR24210. PTHR24210. 1 hit.
PfamPF00412. LIM. 5 hits.
[Graphical view]
PIRSFPIRSF038003. PINCH. 1 hit.
SMARTSM00132. LIM. 5 hits.
[Graphical view]
PROSITEPS00478. LIM_DOMAIN_1. 4 hits.
PS50023. LIM_DOMAIN_2. 5 hits.
[Graphical view]
ProtoNetSearch...

Other

EvolutionaryTraceQ7Z4I7.
GenomeRNAi55679.
NextBio35473432.
PROQ7Z4I7.
SOURCESearch...

Entry information

Entry nameLIMS2_HUMAN
AccessionPrimary (citable) accession number: Q7Z4I7
Secondary accession number(s): A6NLH0 expand/collapse secondary AC list , B4DMV1, F5H6E6, Q7Z4I2, Q7Z4I6, Q7Z4I8, Q8NFE7, Q9HA13
Entry history
Integrated into UniProtKB/Swiss-Prot: December 12, 2006
Last sequence update: October 1, 2003
Last modified: July 9, 2014
This is version 92 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Relevant documents

SIMILARITY comments

Index of protein domains and families

PDB cross-references

Index of Protein Data Bank (PDB) cross-references

MIM cross-references

Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot

Human chromosome 2

Human chromosome 2: entries, gene names and cross-references to MIM