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Protein

ATP-dependent RNA helicase DHX29

Gene

DHX29

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

ATP-binding RNA helicase involved in translation initiation. Part of the 43S pre-initiation complex that is required for efficient initiation on mRNAs of higher eukaryotes with structured 5'-UTRs by promoting efficient NTPase-dependent 48S complex formation. Specifically binds to the 40S ribosome near the mRNA entrance. Does not possess a processive helicase activity.2 Publications

Catalytic activityi

ATP + H2O = ADP + phosphate.

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi595 – 602ATP8

GO - Molecular functioni

  • ATP binding Source: UniProtKB-KW
  • ATP-dependent RNA helicase activity Source: GO_Central
  • cadherin binding Source: BHF-UCL
  • ribosomal small subunit binding Source: UniProtKB
  • RNA binding Source: UniProtKB
  • translation initiation factor activity Source: UniProtKB-KW

GO - Biological processi

Keywordsi

Molecular functionHelicase, Hydrolase, Initiation factor
Biological processProtein biosynthesis
LigandATP-binding, Nucleotide-binding

Names & Taxonomyi

Protein namesi
Recommended name:
ATP-dependent RNA helicase DHX29 (EC:3.6.4.13)
Alternative name(s):
DEAH box protein 29
Nucleic acid helicase DDXx
Gene namesi
Name:DHX29
Synonyms:DDX29
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
Proteomesi
  • UP000005640 Componenti: Chromosome 5

Organism-specific databases

EuPathDBiHostDB:ENSG00000067248.9.
HGNCiHGNC:15815. DHX29.

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Cytoplasm

Pathology & Biotechi

Organism-specific databases

DisGeNETi54505.
OpenTargetsiENSG00000067248.
PharmGKBiPA27215.

Polymorphism and mutation databases

BioMutaiDHX29.
DMDMi110278938.

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00002455351 – 1369ATP-dependent RNA helicase DHX29Add BLAST1369

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei71PhosphoserineCombined sources1
Modified residuei192PhosphoserineCombined sources1
Modified residuei200PhosphoserineCombined sources1

Keywords - PTMi

Phosphoprotein

Proteomic databases

EPDiQ7Z478.
MaxQBiQ7Z478.
PaxDbiQ7Z478.
PeptideAtlasiQ7Z478.
PRIDEiQ7Z478.

PTM databases

iPTMnetiQ7Z478.
PhosphoSitePlusiQ7Z478.

Expressioni

Gene expression databases

BgeeiENSG00000067248.
CleanExiHS_DHX29.
ExpressionAtlasiQ7Z478. baseline and differential.
GenevisibleiQ7Z478. HS.

Organism-specific databases

HPAiHPA038317.
HPA038318.

Interactioni

Subunit structurei

Part of the 43S pre-initiation complex (PIC) that contains at least Met-tRNA, EIF1, EIF1A (EIF1AX or EIF1AY), EIF2S1, EIF2S2, EIF2S3, EIF3A, EIF3B, EIF3C, EIF3D, EIF3E, EIF3F, EIF3G, EIF3H, EIF3I, EIF3J, EIF3K, EIF3L, EIF3M, DHX29 and the 40S ribosomal subunit.1 Publication

GO - Molecular functioni

  • cadherin binding Source: BHF-UCL

Protein-protein interaction databases

BioGridi120001. 29 interactors.
IntActiQ7Z478. 14 interactors.
MINTiMINT-1376685.
STRINGi9606.ENSP00000251636.

Structurei

3D structure databases

ProteinModelPortaliQ7Z478.
SMRiQ7Z478.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini582 – 755Helicase ATP-bindingAdd BLAST174
Domaini849 – 1026Helicase C-terminalAdd BLAST178

Coiled coil

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Coiled coili222 – 256Add BLAST35
Coiled coili283 – 310Add BLAST28
Coiled coili492 – 519Add BLAST28

Motif

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Motifi702 – 705DEAH box4

Compositional bias

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Compositional biasi10 – 53Ala-richAdd BLAST44
Compositional biasi789 – 793Poly-Glu5

Sequence similaritiesi

Belongs to the DEAD box helicase family. DEAH subfamily.

Keywords - Domaini

Coiled coil

Phylogenomic databases

eggNOGiKOG0920. Eukaryota.
COG1643. LUCA.
GeneTreeiENSGT00760000119189.
HOGENOMiHOG000247063.
HOVERGENiHBG081436.
InParanoidiQ7Z478.
KOiK18995.
PhylomeDBiQ7Z478.
TreeFamiTF324744.

Family and domain databases

CDDicd00079. HELICc. 1 hit.
HAMAPiMF_03068. DHX29. 1 hit.
InterProiView protein in InterPro
IPR011545. DEAD/DEAH_box_helicase_dom.
IPR034730. DHX29.
IPR002464. DNA/RNA_helicase_DEAH_CS.
IPR011709. DUF1605.
IPR007502. Helicase-assoc_dom.
IPR014001. Helicase_ATP-bd.
IPR001650. Helicase_C.
IPR027417. P-loop_NTPase.
PfamiView protein in Pfam
PF00270. DEAD. 1 hit.
PF04408. HA2. 1 hit.
PF00271. Helicase_C. 1 hit.
PF07717. OB_NTP_bind. 1 hit.
SMARTiView protein in SMART
SM00487. DEXDc. 1 hit.
SM00847. HA2. 1 hit.
SM00490. HELICc. 1 hit.
SUPFAMiSSF52540. SSF52540. 2 hits.
PROSITEiView protein in PROSITE
PS00690. DEAH_ATP_HELICASE. 1 hit.
PS51192. HELICASE_ATP_BIND_1. 1 hit.
PS51194. HELICASE_CTER. 1 hit.

Sequencei

Sequence statusi: Complete.

Q7Z478-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MGGKNKKHKA PAAAVVRAAV SASRAKSAEA GIAGEAQSKK PVSRPATAAA
60 70 80 90 100
AAAGSREPRV KQGPKIYSFN STNDSSGPAN LDKSILKVVI NNKLEQRIIG
110 120 130 140 150
VINEHKKQNN DKGMISGRLT AKKLQDLYMA LQAFSFKTKD IEDAMTNTLL
160 170 180 190 200
YGGDLHSALD WLCLNLSDDA LPEGFSQEFE EQQPKSRPKF QSPQIQATIS
210 220 230 240 250
PPLQPKTKTY EEDPKSKPKK EEKNMEVNMK EWILRYAEQQ NEEEKNENSK
260 270 280 290 300
SLEEEEKFDP NERYLHLAAK LLDAKEQAAT FKLEKNKQGQ KEAQEKIRKF
310 320 330 340 350
QREMETLEDH PVFNPAMKIS HQQNERKKPP VATEGESALN FNLFEKSAAA
360 370 380 390 400
TEEEKDKKKE PHDVRNFDYT ARSWTGKSPK QFLIDWVRKN LPKSPNPSFE
410 420 430 440 450
KVPVGRYWKC RVRVIKSEDD VLVVCPTILT EDGMQAQHLG ATLALYRLVK
460 470 480 490 500
GQSVHQLLPP TYRDVWLEWS DAEKKREELN KMETNKPRDL FIAKLLNKLK
510 520 530 540 550
QQQQQQQQHS ENKRENSEDP EESWENLVSD EDFSALSLES ANVEDLEPVR
560 570 580 590 600
NLFRKLQSTP KYQKLLKERQ QLPVFKHRDS IVETLKRHRV VVVAGETGSG
610 620 630 640 650
KSTQVPHFLL EDLLLNEWEA SKCNIVCTQP RRISAVSLAN RVCDELGCEN
660 670 680 690 700
GPGGRNSLCG YQIRMESRAC ESTRLLYCTT GVLLRKLQED GLLSNVSHVI
710 720 730 740 750
VDEVHERSVQ SDFLLIILKE ILQKRSDLHL ILMSATVDSE KFSTYFTHCP
760 770 780 790 800
ILRISGRSYP VEVFHLEDII EETGFVLEKD SEYCQKFLEE EEEVTINVTS
810 820 830 840 850
KAGGIKKYQE YIPVQTGAHA DLNPFYQKYS SRTQHAILYM NPHKINLDLI
860 870 880 890 900
LELLAYLDKS PQFRNIEGAV LIFLPGLAHI QQLYDLLSND RRFYSERYKV
910 920 930 940 950
IALHSILSTQ DQAAAFTLPP PGVRKIVLAT NIAETGITIP DVVFVIDTGR
960 970 980 990 1000
TKENKYHESS QMSSLVETFV SKASALQRQG RAGRVRDGFC FRMYTRERFE
1010 1020 1030 1040 1050
GFMDYSVPEI LRVPLEELCL HIMKCNLGSP EDFLSKALDP PQLQVISNAM
1060 1070 1080 1090 1100
NLLRKIGACE LNEPKLTPLG QHLAALPVNV KIGKMLIFGA IFGCLDPVAT
1110 1120 1130 1140 1150
LAAVMTEKSP FTTPIGRKDE ADLAKSALAM ADSDHLTIYN AYLGWKKARQ
1160 1170 1180 1190 1200
EGGYRSEITY CRRNFLNRTS LLTLEDVKQE LIKLVKAAGF SSSTTSTSWE
1210 1220 1230 1240 1250
GNRASQTLSF QEIALLKAVL VAGLYDNVGK IIYTKSVDVT EKLACIVETA
1260 1270 1280 1290 1300
QGKAQVHPSS VNRDLQTHGW LLYQEKIRYA RVYLRETTLI TPFPVLLFGG
1310 1320 1330 1340 1350
DIEVQHRERL LSIDGWIYFQ APVKIAVIFK QLRVLIDSVL RKKLENPKMS
1360
LENDKILQII TELIKTENN
Length:1,369
Mass (Da):155,236
Last modified:July 11, 2006 - v2
Checksum:iF720A77D224C7F59
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti121A → D in CAH56172 (PubMed:17974005).Curated1
Sequence conflicti185K → E in CAH56153 (PubMed:17974005).Curated1
Sequence conflicti356D → G in AAK64516 (Ref. 1) Curated1
Sequence conflicti885D → G in CAH56172 (PubMed:17974005).Curated1

Natural variant

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Natural variantiVAR_052180309D → A. Corresponds to variant dbSNP:rs35874395Ensembl.1
Natural variantiVAR_026985630P → H1 PublicationCorresponds to variant dbSNP:rs17854904Ensembl.1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AY036974 mRNA. Translation: AAK64516.1.
BC056219 mRNA. Translation: AAH56219.1.
AL834496 mRNA. Translation: CAD39154.1.
BX648101 mRNA. Translation: CAH56172.1.
BX648269 mRNA. Translation: CAH56153.1.
AL079292 mRNA. Translation: CAB45191.1.
AF070639 mRNA. Translation: AAC25394.1.
CCDSiCCDS34158.1.
RefSeqiNP_001332893.1. NM_001345964.1.
NP_001332894.1. NM_001345965.1.
NP_061903.2. NM_019030.3.
UniGeneiHs.593268.

Genome annotation databases

EnsembliENST00000251636; ENSP00000251636; ENSG00000067248.
GeneIDi54505.
KEGGihsa:54505.
UCSCiuc003jpx.4. human.

Keywords - Coding sequence diversityi

Polymorphism

Similar proteinsi

Entry informationi

Entry nameiDHX29_HUMAN
AccessioniPrimary (citable) accession number: Q7Z478
Secondary accession number(s): O75549
, Q63HN0, Q63HN3, Q8IWW2, Q8N3A1, Q9UMH2
Entry historyiIntegrated into UniProtKB/Swiss-Prot: July 11, 2006
Last sequence update: July 11, 2006
Last modified: October 25, 2017
This is version 138 of the entry and version 2 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Human chromosome 5
    Human chromosome 5: entries, gene names and cross-references to MIM
  2. Human entries with polymorphisms or disease mutations
    List of human entries with polymorphisms or disease mutations
  3. Human polymorphisms and disease mutations
    Index of human polymorphisms and disease mutations
  4. MIM cross-references
    Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
  5. SIMILARITY comments
    Index of protein domains and families