Q7Z478 (DHX29_HUMAN) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
ATP-dependent RNA helicase DHX29
EC=3.6.4.13

Alternative name(s):
DEAH box protein 29
Nucleic acid helicase DDXx

Gene names

Name:	DHX29
Synonyms:	DDX29

Organism

Homo sapiens (Human)

Taxonomic identifier

9606 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Primates › Haplorrhini › Catarrhini › Hominidae › Homo

Protein attributes

Sequence length	1369 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	ATP-binding RNA helicase involved in translation initiation. Required for efficient initiation on mammalian mRNAs with structured 5'-UTRs by promoting efficient NTPase-dependent 48S complex formation. Specifically binds to the 40S ribosome near the mRNA entrance. Does not possess a processive helicase activity. Ref.8
Catalytic activity	ATP + H2O = ADP + phosphate.
Subcellular location	Cytoplasm Probable.
Sequence similarities	Belongs to the DEAD box helicase family. DEAH subfamily. Contains 1 helicase ATP-binding domain. Contains 1 helicase C-terminal domain.

Ontologies

Keywords
Biological process	Protein biosynthesis
Cellular component	Cytoplasm
Coding sequence diversity	Polymorphism
Domain	Coiled coil
Ligand	ATP-binding Nucleotide-binding
Molecular function	Helicase Hydrolase Initiation factor
PTM	Phosphoprotein
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Molecular function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW ATP-dependent helicase activity Inferred from electronic annotation. Source: InterPro translation initiation factor activity Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 1369	1369	ATP-dependent RNA helicase DHX29		PRO_0000245535
Regions
	Domain	582 – 755	174	Helicase ATP-binding
	Domain	849 – 1026	178	Helicase C-terminal
	Nucleotide binding	595 – 602	8	ATP By similarity
	Coiled coil	221 – 311	91	Potential
	Coiled coil	492 – 519	28	Potential
	Motif	702 – 705	4	DEAH box
	Compositional bias	10 – 53	44	Ala-rich
	Compositional bias	789 – 793	5	Poly-Glu
Amino acid modifications
	Modified residue	192	1	Phosphoserine Ref.7 Ref.9 Ref.11
	Modified residue	200	1	Phosphoserine Ref.6 Ref.7 Ref.9 Ref.10 Ref.11
Natural variations
	Natural variant	309	1	D → A. Corresponds to variant rs35874395 [ dbSNP | Ensembl ].		VAR_052180
	Natural variant	630	1	P → H. Ref.2 Corresponds to variant rs17854904 [ dbSNP | Ensembl ].		VAR_026985
Experimental info
	Sequence conflict	121	1	A → D in CAH56172. Ref.3
	Sequence conflict	185	1	K → E in CAH56153. Ref.3
	Sequence conflict	356	1	D → G in AAK64516. Ref.1
	Sequence conflict	885	1	D → G in CAH56172. Ref.3

Sequences

	Sequence		Length	Mass (Da)
	Q7Z478 [UniParc]. Last modified July 11, 2006. Version 2. Checksum: F720A77D224C7F59	FASTA	1,369	155,236
	10 20 30 40 50 60 MGGKNKKHKA PAAAVVRAAV SASRAKSAEA GIAGEAQSKK PVSRPATAAA AAAGSREPRV 70 80 90 100 110 120 KQGPKIYSFN STNDSSGPAN LDKSILKVVI NNKLEQRIIG VINEHKKQNN DKGMISGRLT 130 140 150 160 170 180 AKKLQDLYMA LQAFSFKTKD IEDAMTNTLL YGGDLHSALD WLCLNLSDDA LPEGFSQEFE 190 200 210 220 230 240 EQQPKSRPKF QSPQIQATIS PPLQPKTKTY EEDPKSKPKK EEKNMEVNMK EWILRYAEQQ 250 260 270 280 290 300 NEEEKNENSK SLEEEEKFDP NERYLHLAAK LLDAKEQAAT FKLEKNKQGQ KEAQEKIRKF 310 320 330 340 350 360 QREMETLEDH PVFNPAMKIS HQQNERKKPP VATEGESALN FNLFEKSAAA TEEEKDKKKE 370 380 390 400 410 420 PHDVRNFDYT ARSWTGKSPK QFLIDWVRKN LPKSPNPSFE KVPVGRYWKC RVRVIKSEDD 430 440 450 460 470 480 VLVVCPTILT EDGMQAQHLG ATLALYRLVK GQSVHQLLPP TYRDVWLEWS DAEKKREELN 490 500 510 520 530 540 KMETNKPRDL FIAKLLNKLK QQQQQQQQHS ENKRENSEDP EESWENLVSD EDFSALSLES 550 560 570 580 590 600 ANVEDLEPVR NLFRKLQSTP KYQKLLKERQ QLPVFKHRDS IVETLKRHRV VVVAGETGSG 610 620 630 640 650 660 KSTQVPHFLL EDLLLNEWEA SKCNIVCTQP RRISAVSLAN RVCDELGCEN GPGGRNSLCG 670 680 690 700 710 720 YQIRMESRAC ESTRLLYCTT GVLLRKLQED GLLSNVSHVI VDEVHERSVQ SDFLLIILKE 730 740 750 760 770 780 ILQKRSDLHL ILMSATVDSE KFSTYFTHCP ILRISGRSYP VEVFHLEDII EETGFVLEKD 790 800 810 820 830 840 SEYCQKFLEE EEEVTINVTS KAGGIKKYQE YIPVQTGAHA DLNPFYQKYS SRTQHAILYM 850 860 870 880 890 900 NPHKINLDLI LELLAYLDKS PQFRNIEGAV LIFLPGLAHI QQLYDLLSND RRFYSERYKV 910 920 930 940 950 960 IALHSILSTQ DQAAAFTLPP PGVRKIVLAT NIAETGITIP DVVFVIDTGR TKENKYHESS 970 980 990 1000 1010 1020 QMSSLVETFV SKASALQRQG RAGRVRDGFC FRMYTRERFE GFMDYSVPEI LRVPLEELCL 1030 1040 1050 1060 1070 1080 HIMKCNLGSP EDFLSKALDP PQLQVISNAM NLLRKIGACE LNEPKLTPLG QHLAALPVNV 1090 1100 1110 1120 1130 1140 KIGKMLIFGA IFGCLDPVAT LAAVMTEKSP FTTPIGRKDE ADLAKSALAM ADSDHLTIYN 1150 1160 1170 1180 1190 1200 AYLGWKKARQ EGGYRSEITY CRRNFLNRTS LLTLEDVKQE LIKLVKAAGF SSSTTSTSWE 1210 1220 1230 1240 1250 1260 GNRASQTLSF QEIALLKAVL VAGLYDNVGK IIYTKSVDVT EKLACIVETA QGKAQVHPSS 1270 1280 1290 1300 1310 1320 VNRDLQTHGW LLYQEKIRYA RVYLRETTLI TPFPVLLFGG DIEVQHRERL LSIDGWIYFQ 1330 1340 1350 1360 APVKIAVIFK QLRVLIDSVL RKKLENPKMS LENDKILQII TELIKTENN

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Identification of a new member of the DDx subfamily of helicases." Abdelhaleem M.M. Submitted (MAY-2001) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [MRNA].
[2]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed: 15489334] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA], VARIANT HIS-630. Tissue: Brain.
[3]	"The full-ORF clone resource of the German cDNA consortium." Bechtel S., Rosenfelder H., Duda A., Schmidt C.P., Ernst U., Wellenreuther R., Mehrle A., Schuster C., Bahr A., Bloecker H., Heubner D., Hoerlein A., Michel G., Wedler H., Koehrer K., Ottenwaelder B., Poustka A., Wiemann S., Schupp I. BMC Genomics 8:399-399(2007) [PubMed: 17974005] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 57-1369. Tissue: Amygdala, Fetal kidney and Testis.
[4]	Bassi M.T., Banfi S., Riboni M., Ballabio A., Borsani G. Submitted (JUL-2000) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 561-1369.
[5]	Yu W., Gibbs R.A. Submitted (JUN-1998) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 1119-1369. Tissue: Brain.
[6]	"Large-scale characterization of HeLa cell nuclear phosphoproteins." Beausoleil S.A., Jedrychowski M., Schwartz D., Elias J.E., Villen J., Li J., Cohn M.A., Cantley L.C., Gygi S.P. Proc. Natl. Acad. Sci. U.S.A. 101:12130-12135(2004) [PubMed: 15302935] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-200, MASS SPECTROMETRY. Tissue: Cervix carcinoma.
[7]	"A probability-based approach for high-throughput protein phosphorylation analysis and site localization." Beausoleil S.A., Villen J., Gerber S.A., Rush J., Gygi S.P. Nat. Biotechnol. 24:1285-1292(2006) [PubMed: 16964243] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-192 AND SER-200, MASS SPECTROMETRY. Tissue: Cervix carcinoma.
[8]	"Translation initiation on mammalian mRNAs with structured 5'UTRs requires DExH-box protein DHX29." Pisareva V.P., Pisarev A.V., Komar A.A., Hellen C.U.T., Pestova T.V. Cell 135:1237-1250(2008) [PubMed: 19109895] [Abstract] Cited for: FUNCTION, RIBOSOME-BINDING, IDENTIFICATION BY MASS SPECTROMETRY.
[9]	"A quantitative atlas of mitotic phosphorylation." Dephoure N., Zhou C., Villen J., Beausoleil S.A., Bakalarski C.E., Elledge S.J., Gygi S.P. Proc. Natl. Acad. Sci. U.S.A. 105:10762-10767(2008) [PubMed: 18669648] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-192 AND SER-200, MASS SPECTROMETRY. Tissue: Cervix carcinoma.
[10]	"Lys-N and trypsin cover complementary parts of the phosphoproteome in a refined SCX-based approach." Gauci S., Helbig A.O., Slijper M., Krijgsveld J., Heck A.J., Mohammed S. Anal. Chem. 81:4493-4501(2009) [PubMed: 19413330] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-200, MASS SPECTROMETRY. Tissue: Embryonic kidney.
[11]	"Quantitative phosphoproteomic analysis of T cell receptor signaling reveals system-wide modulation of protein-protein interactions." Mayya V., Lundgren D.H., Hwang S.-I., Rezaul K., Wu L., Eng J.K., Rodionov V., Han D.K. Sci. Signal. 2:RA46-RA46(2009) [PubMed: 19690332] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-192 AND SER-200, MASS SPECTROMETRY. Tissue: Leukemic T-cell.
[12]	"Initial characterization of the human central proteome." Burkard T.R., Planyavsky M., Kaupe I., Breitwieser F.P., Buerckstuemmer T., Bennett K.L., Superti-Furga G., Colinge J. BMC Syst. Biol. 5:17-17(2011) [PubMed: 21269460] [Abstract] Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AY036974 mRNA. Translation: AAK64516.1. BC056219 mRNA. Translation: AAH56219.1. AL834496 mRNA. Translation: CAD39154.1. BX648101 mRNA. Translation: CAH56172.1. BX648269 mRNA. Translation: CAH56153.1. AL079292 mRNA. Translation: CAB45191.1. AF070639 mRNA. Translation: AAC25394.1.
IPI	IPI00217413.
RefSeq	NP_061903.2. NM_019030.2.
UniGene	Hs.593268.
3D structure databases
ProteinModelPortal	Q7Z478.
SMR	Q7Z478. Positions 691-740, 867-997, 1043-1302.
ModBase	Search...
Protein-protein interaction databases
IntAct	Q7Z478. 3 interactions.
MINT	MINT-1376685.
STRING	Q7Z478.
PTM databases
PhosphoSite	Q7Z478.
Polymorphism databases
DMDM	110278938.
Proteomic databases
PeptideAtlas	Q7Z478.
PRIDE	Q7Z478.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
Ensembl	ENST00000251636; ENSP00000251636; ENSG00000067248.
GeneID	54505.
KEGG	hsa:54505.
UCSC	uc003jpx.1. human.
Organism-specific databases
CTD	54505.
GeneCards	GC05M054552.
HGNC	HGNC:15815. DHX29.
HPA	HPA038317. HPA038318.
MIM	612720. gene.
neXtProt	NX_Q7Z478.
PharmGKB	PA27215.
GenAtlas	Search...
Phylogenomic databases
eggNOG	prNOG05232.
GeneTree	ENSGT00550000074257.
HOVERGEN	HBG081436.
InParanoid	Q7Z478.
OMA	YWKCRVR.
OrthoDB	EOG43FGW2.
PhylomeDB	Q7Z478.
Gene expression databases
ArrayExpress	Q7Z478.
Bgee	Q7Z478.
CleanEx	HS_DHX29.
Genevestigator	Q7Z478.
GermOnline	ENSG00000067248. Homo sapiens.
Family and domain databases
InterPro	IPR014001. DEAD-like_helicase. IPR011545. DNA/RNA_helicase_DEAD/DEAH_N. IPR002464. DNA/RNA_helicase_DEAH_CS. IPR011709. DUF1605. IPR007502. Helicase-assoc_dom. IPR001650. Helicase_C. [Graphical view]
Pfam	PF00270. DEAD. 1 hit. PF04408. HA2. 1 hit. PF00271. Helicase_C. 1 hit. PF07717. OB_NTP_bind. 1 hit. [Graphical view]
SMART	SM00487. DEXDc. 1 hit. SM00847. HA2. 1 hit. SM00490. HELICc. 1 hit. [Graphical view]
PROSITE	PS00690. DEAH_ATP_HELICASE. 1 hit. PS51192. HELICASE_ATP_BIND_1. 1 hit. PS51194. HELICASE_CTER. 1 hit. [Graphical view]
ProtoNet	Search...
Other
NextBio	56869.
SOURCE	Search...

Entry information

Entry name

DHX29_HUMAN

Accession

Primary (citable) accession number: Q7Z478
Secondary accession number(s): O75549 , Q63HN0, Q63HN3, Q8IWW2, Q8N3A1, Q9UMH2

Entry history

Integrated into UniProtKB/Swiss-Prot:	July 11, 2006
Last sequence update:	July 11, 2006
Last modified:	January 25, 2012
This is version 83 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program

Disclaimer

Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.