Q7Z449 (CP2U1_HUMAN) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Cytochrome P450 2U1
EC=1.14.14.1

Gene names

Name:

CYP2U1

Organism

Homo sapiens (Human) [Reference proteome]

Taxonomic identifier

9606 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Deuterostomia › Chordata › Craniata › Vertebrata › Gnathostomata › Teleostomi › Euteleostomi › Sarcopterygii › Tetrapoda › Amniota › Mammalia › Theria › Eutheria › Euarchontoglires › Primates › Haplorrhini › Simiiformes › Catarrhini › Hominoidea › Hominidae › Homininae › Homo

Protein attributes

Sequence length	544 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Catalyzes the hydroxylation of arachidonic acid, docosahexaenoic acid and other long chain fatty acids. May modulate the arachidonic acid signaling pathway and play a role in other fatty acid signaling processes. Ref.1
Catalytic activity	RH + reduced flavoprotein + O2 = ROH + oxidized flavoprotein + H2O.
Cofactor	Heme group By similarity.
Subcellular location	Endoplasmic reticulum membrane; Multi-pass membrane protein. Microsome membrane Ref.1.
Tissue specificity	Widely expressed with stronger expression in thymus, heart and cerebellum. Ref.1 Ref.4 Ref.5
Developmental stage	Expressed in fetal thymus. Ref.1 Ref.5
Sequence similarities	Belongs to the cytochrome P450 family.
Biophysicochemical properties	Kinetic parameters: KM=2.7 µM for arachidonic acid

Ontologies

Keywords
Cellular component	Endoplasmic reticulum Membrane Microsome
Coding sequence diversity	Alternative splicing
Domain	Transmembrane Transmembrane helix
Ligand	Heme Iron Metal-binding NADP
Molecular function	Monooxygenase Oxidoreductase
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	small molecule metabolic process Traceable author statement. Source: Reactome xenobiotic metabolic process Traceable author statement. Source: Reactome
Cellular_component	endoplasmic reticulum membrane Traceable author statement. Source: Reactome integral to membrane Inferred from electronic annotation. Source: UniProtKB-KW
Molecular_function	aromatase activity Inferred from electronic annotation. Source: EC electron carrier activity Inferred from electronic annotation. Source: InterPro heme binding Inferred from electronic annotation. Source: InterPro iron ion binding Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Alternative products

This entry describes 2 isoforms produced by alternative splicing. [Align] [Select]

Isoform 1 (identifier: Q7Z449-1)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

Isoform 2 (identifier: Q7Z449-2)

The sequence of this isoform differs from the canonical sequence as follows:
     164-168: GVVFA → ELFQE
     169-544: Missing.

Note: No experimental confirmation available.

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 544	544	Cytochrome P450 2U1		PRO_0000291756
Regions
	Transmembrane	30 – 50	21	Helical; Potential
	Transmembrane	113 – 133	21	Helical; Potential
	Transmembrane	261 – 281	21	Helical; Potential
	Transmembrane	342 – 362	21	Helical; Potential
	Transmembrane	495 – 515	21	Helical; Potential
Sites
	Metal binding	490	1	Iron (heme axial ligand) By similarity
Natural variations
	Alternative sequence	164 – 168	5	GVVFA → ELFQE in isoform 2.		VSP_026222
	Alternative sequence	169 – 544	376	Missing in isoform 2.		VSP_026223

Sequences

	Sequence		Length	Mass (Da)
	Isoform 1 [UniParc]. Last modified October 1, 2003. Version 1. Checksum: 01A0729318605770	FASTA	544	61,987
	10 20 30 40 50 60 MSSPGPSQPP AEDPPWPARL LRAPLGLLRL DPSGGALLLC GLVALLGWSW LRRRRARGIP 70 80 90 100 110 120 PGPTPWPLVG NFGHVLLPPF LRRRSWLSSR TRAAGIDPSV IGPQVLLAHL ARVYGSIFSF 130 140 150 160 170 180 FIGHYLVVVL SDFHSVREAL VQQAEVFSDR PRVPLISIVT KEKGVVFAHY GPVWRQQRKF 190 200 210 220 230 240 SHSTLRHFGL GKLSLEPKII EEFKYVKAEM QKHGEDPFCP FSIISNAVSN IICSLCFGQR 250 260 270 280 290 300 FDYTNSEFKK MLGFMSRGLE ICLNSQVLLV NICPWLYYLP FGPFKELRQI EKDITSFLKK 310 320 330 340 350 360 IIKDHQESLD RENPQDFIDM YLLHMEEERK NNSNSSFDEE YLFYIIGDLF IAGTDTTTNS 370 380 390 400 410 420 LLWCLLYMSL NPDVQEKVHE EIERVIGANR APSLTDKAQM PYTEATIMEV QRLTVVVPLA 430 440 450 460 470 480 IPHMTSENTV LQGYTIPKGT LILPNLWSVH RDPAIWEKPE DFYPNRFLDD QGQLIKKETF 490 500 510 520 530 540 IPFGIGKRVC MGEQLAKMEL FLMFVSLMQS FAFALPEDSK KPLLTGRFGL TLAPHPFNIT ISRR
	Isoform 2 [UniParc]. Checksum: 1155F0747E11BAFC	FASTA	168	18,578
	10 20 30 40 50 60 MSSPGPSQPP AEDPPWPARL LRAPLGLLRL DPSGGALLLC GLVALLGWSW LRRRRARGIP 70 80 90 100 110 120 PGPTPWPLVG NFGHVLLPPF LRRRSWLSSR TRAAGIDPSV IGPQVLLAHL ARVYGSIFSF 130 140 150 160 FIGHYLVVVL SDFHSVREAL VQQAEVFSDR PRVPLISIVT KEKELFQE

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"CYP2U1, a novel human thymus- and brain-specific cytochrome P450, catalyzes omega- and (omega-1)-hydroxylation of fatty acids." Chuang S.S., Helvig C., Taimi M., Ramshaw H.A., Collop A.H., Amad M., White J.A., Petkovich M., Jones G., Korczak B. J. Biol. Chem. 279:6305-6314(2004) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1), FUNCTION, SUBCELLULAR LOCATION, TISSUE SPECIFICITY, DEVELOPMENTAL STAGE. Tissue: Thymus.
[2]	Mural R.J., Istrail S., Sutton G.G., Florea L., Halpern A.L., Mobarry C.M., Lippert R., Walenz B., Shatkay H., Dew I., Miller J.R., Flanigan M.J., Edwards N.J., Bolanos R., Fasulo D., Halldorsson B.V., Hannenhalli S., Turner R. , Yooseph S., Lu F., Nusskern D.R., Shue B.C., Zheng X.H., Zhong F., Delcher A.L., Huson D.H., Kravitz S.A., Mouchard L., Reinert K., Remington K.A., Clark A.G., Waterman M.S., Eichler E.E., Adams M.D., Hunkapiller M.W., Myers E.W., Venter J.C. Submitted (JUL-2005) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[3]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORMS 1 AND 2). Tissue: Brain and Mammary gland.
[4]	"Characterization and tissue distribution of a novel human cytochrome P450-CYP2U1." Karlgren M., Backlund M., Johansson I., Oscarson M., Ingelman-Sundberg M. Biochem. Biophys. Res. Commun. 315:679-685(2004) [PubMed] [Europe PMC] [Abstract] Cited for: TISSUE SPECIFICITY.
[5]	"Expression patterns of mouse and human CYP orthologs (families 1-4) during development and in different adult tissues." Choudhary D., Jansson I., Stoilov I., Sarfarazi M., Schenkman J.B. Arch. Biochem. Biophys. 436:50-61(2005) [PubMed] [Europe PMC] [Abstract] Cited for: TISSUE SPECIFICITY, DEVELOPMENTAL STAGE.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AY343323 mRNA. Translation: AAQ21380.1. CH471057 Genomic DNA. Translation: EAX06216.1. BC012027 mRNA. Translation: AAH12027.1. BC132767 mRNA. Translation: AAI32768.1. BC136483 mRNA. Translation: AAI36484.1.
IPI	IPI00783946. IPI00847660.
RefSeq	NP_898898.1. NM_183075.2.
UniGene	Hs.109087.
3D structure databases
HSSP	HSSP built from PDB template 2F9Q based on UniProtKB P10635.
ProteinModelPortal	Q7Z449.
ModBase	Search...
Protein-protein interaction databases
IntAct	Q7Z449. 1 interaction.
STRING	9606.ENSP00000333212.
PTM databases
PhosphoSite	Q7Z449.
Polymorphism databases
DMDM	74762432.
Proteomic databases
PaxDb	Q7Z449.
PRIDE	Q7Z449.
Protocols and materials databases
DNASU	113612.
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
Ensembl	ENST00000332884; ENSP00000333212; ENSG00000155016.
GeneID	113612.
KEGG	hsa:113612.
UCSC	uc003hyp.3. human.
Organism-specific databases
CTD	113612.
GeneCards	GC04P108852.
H-InvDB	HIX0024621.
HGNC	HGNC:20582. CYP2U1.
HPA	HPA041622. HPA046754.
MIM	610670. gene.
neXtProt	NX_Q7Z449.
PharmGKB	PA134924269.
GenAtlas	Search...
Phylogenomic databases
eggNOG	COG2124.
HOGENOM	HOG000036991.
HOVERGEN	HBG015789.
InParanoid	Q7Z449.
KO	K07422.
OMA	ICLNSQV.
OrthoDB	EOG476K02.
Enzyme and pathway databases
Reactome	REACT_111217. Metabolism.
Gene expression databases
ArrayExpress	Q7Z449.
Bgee	Q7Z449.
CleanEx	HS_CYP2U1.
Genevestigator	Q7Z449.
Family and domain databases
Gene3D	1.10.630.10. 1 hit.
InterPro	IPR001128. Cyt_P450. IPR017972. Cyt_P450_CS. IPR002401. Cyt_P450_E_grp-I. IPR008069. Cyt_P450_E_grp-I_CYP2D-like. [Graphical view]
Pfam	PF00067. p450. 1 hit. [Graphical view]
PRINTS	PR00463. EP450I. PR01686. EP450ICYP2D. PR00385. P450.
SUPFAM	SSF48264. Cytochrome_P450. 1 hit.
PROSITE	PS00086. CYTOCHROME_P450. 1 hit. [Graphical view]
ProtoNet	Search...
Other
GenomeRNAi	113612.
NextBio	78874.
SOURCE	Search...

Entry information

Entry name

CP2U1_HUMAN

Accession

Primary (citable) accession number: Q7Z449
Secondary accession number(s): B2RMV7, Q96EQ6

Entry history

Integrated into UniProtKB/Swiss-Prot:	June 26, 2007
Last sequence update:	October 1, 2003
Last modified:	May 1, 2013
This is version 97 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program

Disclaimer

Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.