Q7Z417 (NUFP2_HUMAN) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Nuclear fragile X mental retardation-interacting protein 2

Alternative name(s):
82 kDa FMRP-interacting protein
Short name=82-FIP
Cell proliferation-inducing gene 1 protein
FMRP-interacting protein 2

Gene names

Name:	NUFIP2
Synonyms:	KIAA1321
ORF Names:	PIG1

Organism

Homo sapiens (Human) [Reference proteome]

Taxonomic identifier

9606 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Deuterostomia › Chordata › Craniata › Vertebrata › Gnathostomata › Teleostomi › Euteleostomi › Sarcopterygii › Tetrapoda › Amniota › Mammalia › Theria › Eutheria › Euarchontoglires › Primates › Haplorrhini › Simiiformes › Catarrhini › Hominoidea › Hominidae › Homininae › Homo

Protein attributes

Sequence length	695 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Binds RNA. Ref.1
Subunit structure	Interacts with FMRP. Ref.1
Subcellular location	Nucleus. Cytoplasm. Note: Distribution is cell cycle-modulated, being cytoplasmic in the G2/M phase and accumulating in nucleus during the G1 phase. Ref.1
Sequence caution	The sequence BAA92559.1 differs from that shown. Reason: Erroneous initiation.

Ontologies

Keywords
Cellular component	Cytoplasm Nucleus
Ligand	RNA-binding
PTM	Phosphoprotein
Technical term	Complete proteome Direct protein sequencing Reference proteome
Gene Ontology (GO)
Cellular_component	nucleus Inferred from direct assay Ref.1. Source: HGNC polysomal ribosome Inferred from direct assay Ref.1. Source: HGNC
Molecular_function	RNA binding Inferred from direct assay Ref.1. Source: HGNC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 695	695	Nuclear fragile X mental retardation-interacting protein 2		PRO_0000245521
Regions
	Compositional bias	12 – 60	49	His-rich
	Compositional bias	373 – 415	43	Ser-rich
Amino acid modifications
	Modified residue	87	1	Phosphothreonine Ref.9 Ref.16
	Modified residue	112	1	Phosphoserine Ref.13 Ref.16 Ref.18
	Modified residue	113	1	Phosphoserine Ref.13
	Modified residue	212	1	Phosphoserine Ref.8 Ref.13 Ref.15 Ref.16 Ref.18
	Modified residue	214	1	Phosphoserine Ref.13
	Modified residue	218	1	Phosphotyrosine Ref.13
	Modified residue	219	1	Phosphothreonine Ref.13
	Modified residue	220	1	Phosphothreonine Ref.8 Ref.13
	Modified residue	376	1	Phosphoserine Ref.15
	Modified residue	571	1	Phosphothreonine Ref.16
	Modified residue	572	1	Phosphoserine Ref.13 Ref.15 Ref.16 Ref.18
	Modified residue	629	1	Phosphoserine Ref.5 Ref.13 Ref.15 Ref.16 Ref.18
	Modified residue	633	1	Phosphothreonine Ref.16
	Modified residue	637	1	Phosphoserine Ref.13 Ref.15
	Modified residue	652	1	Phosphoserine Ref.6 Ref.7 Ref.12 Ref.13 Ref.14 Ref.16 Ref.18
	Modified residue	655	1	Phosphoserine Ref.18
	Modified residue	692	1	Phosphoserine Ref.13 Ref.16 Ref.18

Sequences

	Sequence		Length	Mass (Da)
	Q7Z417 [UniParc]. Last modified October 1, 2003. Version 1. Checksum: BB5C94131F5B5426	FASTA	695	76,121
	10 20 30 40 50 60 MEEKPGQPQP QHHHSHHHPH HHPQQQQQQP HHHHHYYFYN HSHNHHHHHH HQQPHQYLQH 70 80 90 100 110 120 GAEGSPKAQP KPLKHEQKHT LQQHQETPKK KTGYGELNGN AGEREISLKN LSSDEATNPI 130 140 150 160 170 180 SRVLNGNQQV VDTSLKQTVK ANTFGKAGIK TKNFIQKNSM DKKNGKSYEN KSGENQSVDK 190 200 210 220 230 240 SDTIPIPNGV VTNNSGYITN GYMGKGADND GSGSESGYTT PKKRKARRNS AKGCENLNIV 250 260 270 280 290 300 QDKIMQQETS VPTLKQGLET FKPDYSEQKG NRVDGSKPIW KYETGPGGTS RGKPAVGDML 310 320 330 340 350 360 RKSSDSKPGV SSKKFDDRPK GKHASAVASK EDSWTLFKPP PVFPVDNSSA KIVPKISYAS 370 380 390 400 410 420 KVKENLNKTI QNSSVSPTSS SSSSSSTGET QTQSSSRLSQ VPMSALKSVT SANFSNGPVL 430 440 450 460 470 480 AGTDGNVYPP GGQPLLTTAA NTLTPISSGT DSVLQDMSLT SAAVEQIKTS LFIYPSNMQT 490 500 510 520 530 540 MLLSTAQVDL PSQTDQQNLG DIFQNQWGLS FINEPSAGPE TVTGKSSEHK VMEVTFQGEY 550 560 570 580 590 600 PATLVSQGAE IIPSGTEHPV FPKAYELEKR TSPQVLGSIL KSGTTSESGA LSLEPSHIGD 610 620 630 640 650 660 LQKADTSSQG ALVFLSKDYE IESQNPLASP TNTLLGSAKE QRYQRGLERN DSWGSFDLRA 670 680 690 AIVYHTKEME SIWNLQKQDP KRIITYNEAM DSPDQ

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"82-FIP, a novel FMRP (fragile X mental retardation protein) interacting protein, shows a cell cycle-dependent intracellular localization." Bardoni B., Castets M., Huot M.-E., Schenck A., Adinolfi S., Corbin F., Pastore A., Khandjian E.W., Mandel J.-L. Hum. Mol. Genet. 12:1689-1698(2003) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA], PROTEIN SEQUENCE OF 74-92 AND 593-609, FUNCTION, SUBUNIT, SUBCELLULAR LOCATION, INTERACTION WITH FMRP.
[2]	Kim J.W. Submitted (FEB-2003) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
[3]	"Prediction of the coding sequences of unidentified human genes. XVI. The complete sequences of 150 new cDNA clones from brain which code for large proteins in vitro." Nagase T., Kikuno R., Ishikawa K., Hirosawa M., Ohara O. DNA Res. 7:65-73(2000) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Tissue: Brain.
[4]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Tissue: Uterus.
[5]	Bienvenut W.V., Waridel P., Quadroni M. Submitted (MAR-2009) to UniProtKB Cited for: PROTEIN SEQUENCE OF 92-104; 110-136; 233-269; 331-351; 369-397; 531-563; 571-639; 650-659; 668-677 AND 683-695, PHOSPHORYLATION AT SER-629, MASS SPECTROMETRY. Tissue: Cervix carcinoma.
[6]	"Robust phosphoproteomic profiling of tyrosine phosphorylation sites from human T cells using immobilized metal affinity chromatography and tandem mass spectrometry." Brill L.M., Salomon A.R., Ficarro S.B., Mukherji M., Stettler-Gill M., Peters E.C. Anal. Chem. 76:2763-2772(2004) [PubMed] [Europe PMC] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-652, MASS SPECTROMETRY. Tissue: Leukemic T-cell.
[7]	"Global, in vivo, and site-specific phosphorylation dynamics in signaling networks." Olsen J.V., Blagoev B., Gnad F., Macek B., Kumar C., Mortensen P., Mann M. Cell 127:635-648(2006) [PubMed] [Europe PMC] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-652, MASS SPECTROMETRY. Tissue: Cervix carcinoma.
[8]	"A probability-based approach for high-throughput protein phosphorylation analysis and site localization." Beausoleil S.A., Villen J., Gerber S.A., Rush J., Gygi S.P. Nat. Biotechnol. 24:1285-1292(2006) [PubMed] [Europe PMC] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-212 AND THR-220, MASS SPECTROMETRY. Tissue: Cervix carcinoma.
[9]	"Improved titanium dioxide enrichment of phosphopeptides from HeLa cells and high confident phosphopeptide identification by cross-validation of MS/MS and MS/MS/MS spectra." Yu L.-R., Zhu Z., Chan K.C., Issaq H.J., Dimitrov D.S., Veenstra T.D. J. Proteome Res. 6:4150-4162(2007) [PubMed] [Europe PMC] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT THR-87, MASS SPECTROMETRY. Tissue: Cervix carcinoma.
[10]	"ATM and ATR substrate analysis reveals extensive protein networks responsive to DNA damage." Matsuoka S., Ballif B.A., Smogorzewska A., McDonald E.R. III, Hurov K.E., Luo J., Bakalarski C.E., Zhao Z., Solimini N., Lerenthal Y., Shiloh Y., Gygi S.P., Elledge S.J. Science 316:1160-1166(2007) [PubMed] [Europe PMC] [Abstract] Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS]. Tissue: Embryonic kidney.
[11]	"Combining protein-based IMAC, peptide-based IMAC, and MudPIT for efficient phosphoproteomic analysis." Cantin G.T., Yi W., Lu B., Park S.K., Xu T., Lee J.-D., Yates J.R. III J. Proteome Res. 7:1346-1351(2008) [PubMed] [Europe PMC] [Abstract] Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS]. Tissue: Cervix carcinoma.
[12]	"Kinase-selective enrichment enables quantitative phosphoproteomics of the kinome across the cell cycle." Daub H., Olsen J.V., Bairlein M., Gnad F., Oppermann F.S., Korner R., Greff Z., Keri G., Stemmann O., Mann M. Mol. Cell 31:438-448(2008) [PubMed] [Europe PMC] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-652, MASS SPECTROMETRY. Tissue: Cervix carcinoma.
[13]	"A quantitative atlas of mitotic phosphorylation." Dephoure N., Zhou C., Villen J., Beausoleil S.A., Bakalarski C.E., Elledge S.J., Gygi S.P. Proc. Natl. Acad. Sci. U.S.A. 105:10762-10767(2008) [PubMed] [Europe PMC] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-112; SER-113; SER-212; SER-214; TYR-218; THR-219; THR-220; SER-572; SER-629; SER-637; SER-652 AND SER-692, MASS SPECTROMETRY. Tissue: Cervix carcinoma.
[14]	"Large-scale proteomics analysis of the human kinome." Oppermann F.S., Gnad F., Olsen J.V., Hornberger R., Greff Z., Keri G., Mann M., Daub H. Mol. Cell. Proteomics 8:1751-1764(2009) [PubMed] [Europe PMC] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-652, MASS SPECTROMETRY.
[15]	"Quantitative phosphoproteomic analysis of T cell receptor signaling reveals system-wide modulation of protein-protein interactions." Mayya V., Lundgren D.H., Hwang S.-I., Rezaul K., Wu L., Eng J.K., Rodionov V., Han D.K. Sci. Signal. 2:RA46-RA46(2009) [PubMed] [Europe PMC] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-212; SER-376; SER-572; SER-629 AND SER-637, MASS SPECTROMETRY. Tissue: Leukemic T-cell.
[16]	"Quantitative phosphoproteomics reveals widespread full phosphorylation site occupancy during mitosis." Olsen J.V., Vermeulen M., Santamaria A., Kumar C., Miller M.L., Jensen L.J., Gnad F., Cox J., Jensen T.S., Nigg E.A., Brunak S., Mann M. Sci. Signal. 3:RA3-RA3(2010) [PubMed] [Europe PMC] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT THR-87; SER-112; SER-212; THR-571; SER-572; SER-629; THR-633; SER-652 AND SER-692, MASS SPECTROMETRY. Tissue: Cervix carcinoma.
[17]	"Initial characterization of the human central proteome." Burkard T.R., Planyavsky M., Kaupe I., Breitwieser F.P., Buerckstuemmer T., Bennett K.L., Superti-Furga G., Colinge J. BMC Syst. Biol. 5:17-17(2011) [PubMed] [Europe PMC] [Abstract] Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
[18]	"System-wide temporal characterization of the proteome and phosphoproteome of human embryonic stem cell differentiation." Rigbolt K.T., Prokhorova T.A., Akimov V., Henningsen J., Johansen P.T., Kratchmarova I., Kassem M., Mann M., Olsen J.V., Blagoev B. Sci. Signal. 4:RS3-RS3(2011) [PubMed] [Europe PMC] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-112; SER-212; SER-572; SER-629; SER-652; SER-655 AND SER-692, MASS SPECTROMETRY.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AJ493465 mRNA. Translation: CAD38278.1. AY232289 mRNA. Translation: AAP69984.1. AB037742 mRNA. Translation: BAA92559.1. Different initiation. BC108307 mRNA. Translation: AAI08308.1.
IPI	IPI00002349.
RefSeq	NP_065823.1. NM_020772.2.
UniGene	Hs.462598.
3D structure databases
ProteinModelPortal	Q7Z417.
ModBase	Search...
Protein-protein interaction databases
DIP	DIP-29023N.
IntAct	Q7Z417. 4 interactions.
STRING	9606.ENSP00000225388.
PTM databases
PhosphoSite	Q7Z417.
Polymorphism databases
DMDM	74713454.
Proteomic databases
PaxDb	Q7Z417.
PeptideAtlas	Q7Z417.
PRIDE	Q7Z417.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
Ensembl	ENST00000225388; ENSP00000225388; ENSG00000108256.
GeneID	57532.
KEGG	hsa:57532.
UCSC	uc002hdy.4. human.
Organism-specific databases
CTD	57532.
GeneCards	GC17M027582.
HGNC	HGNC:17634. NUFIP2.
HPA	HPA017344.
MIM	609356. gene.
neXtProt	NX_Q7Z417.
PharmGKB	PA143485564.
HUGE	Search...
GenAtlas	Search...
Phylogenomic databases
eggNOG	NOG85203.
HOGENOM	HOG000220869.
HOVERGEN	HBG065794.
InParanoid	Q7Z417.
OMA	TKEMESV.
OrthoDB	EOG42JNRF.
Gene expression databases
ArrayExpress	Q7Z417.
Bgee	Q7Z417.
CleanEx	HS_NUFIP2.
Genevestigator	Q7Z417.
GermOnline	ENSG00000108256. Homo sapiens.
Family and domain databases
ProtoNet	Search...
Other
ChiTaRS	NUFIP2. human.
GenomeRNAi	57532.
NextBio	63940.
SOURCE	Search...

Entry information

Entry name

NUFP2_HUMAN

Accession

Primary (citable) accession number: Q7Z417
Secondary accession number(s): Q9P2M5

Entry history

Integrated into UniProtKB/Swiss-Prot:	July 11, 2006
Last sequence update:	October 1, 2003
Last modified:	April 3, 2013
This is version 80 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program

Disclaimer

Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.