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Protein

Transmembrane protease serine 9

Gene

TMPRSS9

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Serase-1 and serase-2 are serine proteases that hydrolyze the peptides N-t-Boc-Gln-Ala-Arg-AMC and N-t-Boc-Gln-Gly-Arg-AMC. In contrast, N-t-Boc-Ala-Phe-Lys-AMC and N-t-Boc-Ala-Pro-Ala-AMC are not significantly hydrolyzed.

Enzyme regulationi

Inhibited by serine protease inhibitors PMSF and 4-(2-aminoethyl)benzenesulfonyl fluoride, but not by EDTA.1 Publication

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Active sitei243Charge relay systemBy similarity1
Active sitei292Charge relay systemBy similarity1
Active sitei387Charge relay systemBy similarity1
Active sitei544Charge relay systemBy similarity1
Active sitei592Charge relay systemBy similarity1
Active sitei687Charge relay systemBy similarity1

GO - Molecular functioni

Complete GO annotation...

Keywords - Molecular functioni

Hydrolase, Protease, Serine protease

Protein family/group databases

MEROPSiS01.969.

Names & Taxonomyi

Protein namesi
Recommended name:
Transmembrane protease serine 9 (EC:3.4.21.-)
Alternative name(s):
Polyserase-I
Polyserine protease 1
Short name:
Polyserase-1
Cleaved into the following 3 chains:
Gene namesi
Name:TMPRSS9
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
Proteomesi
  • UP000005640 Componenti: Chromosome 19

Organism-specific databases

HGNCiHGNC:30079. TMPRSS9.

Subcellular locationi

Topology

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Topological domaini1 – 29CytoplasmicSequence analysisAdd BLAST29
Transmembranei30 – 50Helical; Signal-anchor for type II membrane proteinSequence analysisAdd BLAST21
Topological domaini51 – 1059ExtracellularSequence analysisAdd BLAST1009

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cell membrane, Membrane

Pathology & Biotechi

Organism-specific databases

DisGeNETi360200.
OpenTargetsiENSG00000178297.
PharmGKBiPA134967594.

Polymorphism and mutation databases

BioMutaiTMPRSS9.
DMDMi61217609.

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000278671 – 1059Transmembrane protease serine 9Sequence analysisAdd BLAST1059
ChainiPRO_0000027868203 – 503Serase-1Sequence analysisAdd BLAST301
ChainiPRO_0000027869504 – 826Serase-2Sequence analysisAdd BLAST323
ChainiPRO_0000027870827 – 1059Serase-3Sequence analysisAdd BLAST233

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Disulfide bondi154 ↔ 166By similarity
Disulfide bondi161 ↔ 180By similarity
Disulfide bondi174 ↔ 189By similarity
Disulfide bondi228 ↔ 244By similarity
Disulfide bondi326 ↔ 393By similarity
Disulfide bondi358 ↔ 372By similarity
Disulfide bondi383 ↔ 412By similarity
Disulfide bondi529 ↔ 545By similarity
Glycosylationi547N-linked (GlcNAc...)Sequence analysis1
Disulfide bondi626 ↔ 693By similarity
Glycosylationi638N-linked (GlcNAc...)Sequence analysis1
Disulfide bondi658 ↔ 672By similarity
Glycosylationi663N-linked (GlcNAc...)Sequence analysis1
Disulfide bondi683 ↔ 712By similarity
Glycosylationi786N-linked (GlcNAc...)Sequence analysis1
Disulfide bondi853 ↔ 869By similarity
Disulfide bondi949 ↔ 1015By similarity
Disulfide bondi980 ↔ 994By similarity
Disulfide bondi1005 ↔ 1034By similarity

Post-translational modificationi

Proteolytically cleaved to generate 3 independent serine protease chains. The cleaved chains may remain attached to the membrane thanks to disufide bonds. It is unclear whether cleavage always takes place.1 Publication

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sitei202 – 203CleavageSequence analysis2
Sitei503 – 504CleavageSequence analysis2
Sitei826 – 827CleavageSequence analysis2

Keywords - PTMi

Disulfide bond, Glycoprotein

Proteomic databases

PaxDbiQ7Z410.
PeptideAtlasiQ7Z410.
PRIDEiQ7Z410.
TopDownProteomicsiQ7Z410.

PTM databases

iPTMnetiQ7Z410.
PhosphoSitePlusiQ7Z410.

Expressioni

Tissue specificityi

Expressed in fetal human tissues, such as kidney, liver, lung and brain, and in a variety of tumor cell lines. Weakly expressed in adult tissues including skeletal muscle, liver, placenta and heart.1 Publication

Gene expression databases

BgeeiENSG00000178297.
CleanExiHS_TMPRSS9.
ExpressionAtlasiQ7Z410. baseline and differential.

Organism-specific databases

HPAiHPA051483.

Structurei

3D structure databases

ProteinModelPortaliQ7Z410.
SMRiQ7Z410.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini153 – 190LDL-receptor class APROSITE-ProRule annotationAdd BLAST38
Domaini203 – 436Peptidase S1 1PROSITE-ProRule annotationAdd BLAST234
Domaini504 – 736Peptidase S1 2PROSITE-ProRule annotationAdd BLAST233
Domaini827 – 1058Peptidase S1 3PROSITE-ProRule annotationAdd BLAST232

Domaini

The serine protease 1 and 2 domains are catalytically active, whereas the serine protease 3 domain lacks the essential Ser residue of the catalytic triad at position 1009 and is predicted to be inactive.

Sequence similaritiesi

Belongs to the peptidase S1 family.PROSITE-ProRule annotation
Contains 1 LDL-receptor class A domain.PROSITE-ProRule annotation
Contains 3 peptidase S1 domains.PROSITE-ProRule annotation

Keywords - Domaini

Repeat, Signal-anchor, Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiKOG3627. Eukaryota.
COG5640. LUCA.
GeneTreeiENSGT00760000118810.
HOGENOMiHOG000154654.
HOVERGENiHBG079270.
InParanoidiQ7Z410.
KOiK09640.
OMAiVFNKYIQ.
OrthoDBiEOG091G0DF7.
PhylomeDBiQ7Z410.
TreeFamiTF330647.

Family and domain databases

CDDicd00190. Tryp_SPc. 3 hits.
Gene3Di4.10.400.10. 1 hit.
InterProiIPR002172. LDrepeatLR_classA_rpt.
IPR009003. Peptidase_S1_PA.
IPR001314. Peptidase_S1A.
IPR017324. Tmprss9.
IPR001254. Trypsin_dom.
IPR018114. TRYPSIN_HIS.
IPR033116. TRYPSIN_SER.
[Graphical view]
PfamiPF00057. Ldl_recept_a. 1 hit.
PF00089. Trypsin. 3 hits.
[Graphical view]
PIRSFiPIRSF037931. TMPRSS9_polyserase-1. 1 hit.
PRINTSiPR00722. CHYMOTRYPSIN.
SMARTiSM00192. LDLa. 1 hit.
SM00020. Tryp_SPc. 3 hits.
[Graphical view]
SUPFAMiSSF50494. SSF50494. 3 hits.
SSF57424. SSF57424. 1 hit.
PROSITEiPS01209. LDLRA_1. 1 hit.
PS50068. LDLRA_2. 1 hit.
PS50240. TRYPSIN_DOM. 3 hits.
PS00134. TRYPSIN_HIS. 3 hits.
PS00135. TRYPSIN_SER. 2 hits.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q7Z410-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MEPTVADVHL VPRTTKEVPA LDAACCRAAS IGVVATSLVV LTLGVLLAFL
60 70 80 90 100
STQGFHVDHT AELRGIRWTS SLRRETSDYH RTLTPTLEAL LHFLLRPLQT
110 120 130 140 150
LSLGLEEELL QRGIRARLRE HGISLAAYGT IVSAELTGRH KGPLAERDFK
160 170 180 190 200
SGRCPGNSFS CGNSQCVTKV NPECDDQEDC SDGSDEAHCE CGLQPAWRMA
210 220 230 240 250
GRIVGGMEAS PGEFPWQASL RENKEHFCGA AIINARWLVS AAHCFNEFQD
260 270 280 290 300
PTKWVAYVGA TYLSGSEAST VRAQVVQIVK HPLYNADTAD FDVAVLELTS
310 320 330 340 350
PLPFGRHIQP VCLPAATHIF PPSKKCLISG WGYLKEDFLV KPEVLQKATV
360 370 380 390 400
ELLDQALCAS LYGHSLTDRM VCAGYLDGKV DSCQGDSGGP LVCEEPSGRF
410 420 430 440 450
FLAGIVSWGI GCAEARRPGV YARVTRLRDW ILEATTKASM PLAPTMAPAP
460 470 480 490 500
AAPSTAWPTS PESPVVSTPT KSMQALSTVP LDWVTVPKLQ ECGARPAMEK
510 520 530 540 550
PTRVVGGFGA ASGEVPWQVS LKEGSRHFCG ATVVGDRWLL SAAHCFNHTK
560 570 580 590 600
VEQVRAHLGT ASLLGLGGSP VKIGLRRVVL HPLYNPGILD FDLAVLELAS
610 620 630 640 650
PLAFNKYIQP VCLPLAIQKF PVGRKCMISG WGNTQEGNAT KPELLQKASV
660 670 680 690 700
GIIDQKTCSV LYNFSLTDRM ICAGFLEGKV DSCQGDSGGP LACEEAPGVF
710 720 730 740 750
YLAGIVSWGI GCAQVKKPGV YTRITRLKGW ILEIMSSQPL PMSPPSTTRM
760 770 780 790 800
LATTSPRTTA GLTVPGATPS RPTPGAASRV TGQPANSTLS AVSTTARGQT
810 820 830 840 850
PFPDAPEATT HTQLPDCGLA PAALTRIVGG SAAGRGEWPW QVSLWLRRRE
860 870 880 890 900
HRCGAVLVAE RWLLSAAHCF DVYGDPKQWA AFLGTPFLSG AEGQLERVAR
910 920 930 940 950
IYKHPFYNLY TLDYDVALLE LAGPVRRSRL VRPICLPEPA PRPPDGTRCV
960 970 980 990 1000
ITGWGSVREG GSMARQLQKA AVRLLSEQTC RRFYPVQISS RMLCAGFPQG
1010 1020 1030 1040 1050
GVDSCSGDAG GPLACREPSG RWVLTGVTSW GYGCGRPHFP GVYTRVAAVR

GWIGQHIQE
Length:1,059
Mass (Da):114,021
Last modified:March 15, 2005 - v2
Checksum:i17D27A2D99F2A264
GO

Sequence cautioni

The sequence BAD18439 differs from that shown. Reason: Frameshift at position 776.Curated
The sequence CAD35759 differs from that shown. Aberrant splicing.Curated

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti657T → P in BAD18439 (PubMed:14702039).Curated1
Sequence conflicti899A → V in BAD18439 (PubMed:14702039).Curated1

Natural variant

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Natural variantiVAR_0518454T → A.Corresponds to variant rs8100709dbSNPEnsembl.1
Natural variantiVAR_02150830S → T.Corresponds to variant rs891174dbSNPEnsembl.1
Natural variantiVAR_03365073R → W.Corresponds to variant rs17685098dbSNPEnsembl.1
Natural variantiVAR_051846456A → T.Corresponds to variant rs10153474dbSNPEnsembl.1
Natural variantiVAR_061774659S → T.Corresponds to variant rs60568869dbSNPEnsembl.1
Natural variantiVAR_021509793S → N.1 PublicationCorresponds to variant rs735911dbSNPEnsembl.1
Natural variantiVAR_021510938E → K.1 PublicationCorresponds to variant rs7247162dbSNPEnsembl.1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AJ488946 mRNA. Translation: CAD35758.1.
AJ488947 mRNA. Translation: CAD35759.1. Sequence problems.
AC011522 Genomic DNA. No translation available.
AC011542 Genomic DNA. No translation available.
AK131261 mRNA. Translation: BAD18439.1. Frameshift.
CCDSiCCDS12088.1.
RefSeqiNP_892018.1. NM_182973.2.
UniGeneiHs.465560.

Genome annotation databases

EnsembliENST00000332578; ENSP00000330264; ENSG00000178297.
GeneIDi360200.
KEGGihsa:360200.
UCSCiuc010xgx.3. human.

Keywords - Coding sequence diversityi

Polymorphism

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AJ488946 mRNA. Translation: CAD35758.1.
AJ488947 mRNA. Translation: CAD35759.1. Sequence problems.
AC011522 Genomic DNA. No translation available.
AC011542 Genomic DNA. No translation available.
AK131261 mRNA. Translation: BAD18439.1. Frameshift.
CCDSiCCDS12088.1.
RefSeqiNP_892018.1. NM_182973.2.
UniGeneiHs.465560.

3D structure databases

ProteinModelPortaliQ7Z410.
SMRiQ7Z410.
ModBaseiSearch...
MobiDBiSearch...

Protein family/group databases

MEROPSiS01.969.

PTM databases

iPTMnetiQ7Z410.
PhosphoSitePlusiQ7Z410.

Polymorphism and mutation databases

BioMutaiTMPRSS9.
DMDMi61217609.

Proteomic databases

PaxDbiQ7Z410.
PeptideAtlasiQ7Z410.
PRIDEiQ7Z410.
TopDownProteomicsiQ7Z410.

Protocols and materials databases

DNASUi360200.
Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENST00000332578; ENSP00000330264; ENSG00000178297.
GeneIDi360200.
KEGGihsa:360200.
UCSCiuc010xgx.3. human.

Organism-specific databases

CTDi360200.
DisGeNETi360200.
GeneCardsiTMPRSS9.
HGNCiHGNC:30079. TMPRSS9.
HPAiHPA051483.
MIMi610477. gene.
neXtProtiNX_Q7Z410.
OpenTargetsiENSG00000178297.
PharmGKBiPA134967594.
GenAtlasiSearch...

Phylogenomic databases

eggNOGiKOG3627. Eukaryota.
COG5640. LUCA.
GeneTreeiENSGT00760000118810.
HOGENOMiHOG000154654.
HOVERGENiHBG079270.
InParanoidiQ7Z410.
KOiK09640.
OMAiVFNKYIQ.
OrthoDBiEOG091G0DF7.
PhylomeDBiQ7Z410.
TreeFamiTF330647.

Miscellaneous databases

GenomeRNAii360200.
PROiQ7Z410.
SOURCEiSearch...

Gene expression databases

BgeeiENSG00000178297.
CleanExiHS_TMPRSS9.
ExpressionAtlasiQ7Z410. baseline and differential.

Family and domain databases

CDDicd00190. Tryp_SPc. 3 hits.
Gene3Di4.10.400.10. 1 hit.
InterProiIPR002172. LDrepeatLR_classA_rpt.
IPR009003. Peptidase_S1_PA.
IPR001314. Peptidase_S1A.
IPR017324. Tmprss9.
IPR001254. Trypsin_dom.
IPR018114. TRYPSIN_HIS.
IPR033116. TRYPSIN_SER.
[Graphical view]
PfamiPF00057. Ldl_recept_a. 1 hit.
PF00089. Trypsin. 3 hits.
[Graphical view]
PIRSFiPIRSF037931. TMPRSS9_polyserase-1. 1 hit.
PRINTSiPR00722. CHYMOTRYPSIN.
SMARTiSM00192. LDLa. 1 hit.
SM00020. Tryp_SPc. 3 hits.
[Graphical view]
SUPFAMiSSF50494. SSF50494. 3 hits.
SSF57424. SSF57424. 1 hit.
PROSITEiPS01209. LDLRA_1. 1 hit.
PS50068. LDLRA_2. 1 hit.
PS50240. TRYPSIN_DOM. 3 hits.
PS00134. TRYPSIN_HIS. 3 hits.
PS00135. TRYPSIN_SER. 2 hits.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiTMPS9_HUMAN
AccessioniPrimary (citable) accession number: Q7Z410
Secondary accession number(s): Q6ZND6, Q7Z411
Entry historyi
Integrated into UniProtKB/Swiss-Prot: March 15, 2005
Last sequence update: March 15, 2005
Last modified: November 2, 2016
This is version 119 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Human chromosome 19
    Human chromosome 19: entries, gene names and cross-references to MIM
  2. Human entries with polymorphisms or disease mutations
    List of human entries with polymorphisms or disease mutations
  3. Human polymorphisms and disease mutations
    Index of human polymorphisms and disease mutations
  4. MIM cross-references
    Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
  5. Peptidase families
    Classification of peptidase families and list of entries
  6. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.