Reviewed, UniProtKB/Swiss-Prot Q7Z401 (MYCPP_HUMAN)

Names and origin

Protein names

Recommended name:
    C-myc promoter-binding protein
Alternative name(s):
    DENN domain-containing protein 4A

Gene names

Name:	DENND4A
Synonyms:	IRLB, MYCPBP

Organism

Homo sapiens (Human)

Taxonomic identifier

9606 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Primates › Haplorrhini › Catarrhini › Hominidae › Homo

Protein attributes

Sequence length	1863 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at protein level.

General annotation (Comments)

Function	Binds to ISRE-like element (interferon-stimulated response element) of MYC P2 promoter.
Subcellular location	NucleusProbable.
Tissue specificity	Expressed ubiquitously. Highest expression in bone marrow, medium in peripheral blood lymphocytes and lowest in spleen. In brain, breast, and prostate, higher expression was seen in normal cells than in tumor cells. Expression is regulated in a growth- and cell cycle-dependent manner.
Developmental stage	Highly expressed in fetal liver.
Induction	Induced by serum in low-passage fibroblasts.
Sequence similarities	Contains 1 dDENN domain. Contains 1 DENN domain. Contains 2 PPR (pentatricopeptide) repeats. Contains 1 uDENN domain.

Ontologies

Keywords
Biological process	Transcription Transcription regulation
Cellular component	Nucleus
Coding sequence diversity	Polymorphism
Domain	Repeat
Ligand	DNA-binding
PTM	Phosphoprotein
Gene Ontology (GO)
Biological process	regulation of transcription, DNA-dependent Ref.5 Non-traceable author statement. Source: UniProtKB
Cellular component	nucleus Non-traceable author statement. Source: UniProtKB
Molecular function	DNA binding Ref.5 Non-traceable author statement. Source: UniProtKB protein binding Inferred from physical interaction. Source: IntAct
Complete GO annotation...

Binary interactions

With	Entry	#Exp.	IntAct	Notes
YWHAB	P31946	1	EBI-1046479,EBI-359815
YWHAG	P61981	1	EBI-1046479,EBI-359832
YWHAQ	P27348	1	EBI-1046479,EBI-359854

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 1863	1863	C-myc promoter-binding protein		PRO_0000223952
Regions
	Domain	6 – 282	277	uDENN
	Domain	308 – 492	185	DENN
	Domain	558 – 632	75	dDENN
	Repeat	772 – 808	37	PPR 1
	Repeat	809 – 843	35	PPR 2
	Motif	917 – 933	17	Bipartite nuclear localization signal Potential
	Compositional bias	1183 – 1189	7	Poly-Glu
Amino acid modifications
	Modified residue	728	1	Phosphoserine
	Modified residue	731	1	Phosphoserine
	Modified residue	1281	1	Phosphoserine
	Modified residue	1511	1	Phosphoserine
	Modified residue	1587	1	Phosphoserine
Natural variations
	Natural variant	284	1	L → P: dbSNP rs17854146.		VAR_025362
Experimental info
	Sequence conflict	383	1	S → P in CAD89960. Ref.4
	Sequence conflict	983	1	M → MGK in AAQ10514. Ref.1

Sequences

	Sequence		Length	Mass (Da)
	Q7Z401-1 [UniParc]. Last modified February 21, 2006. Version 2. Checksum: 7432EEC9DB9711E6	FASTA	1,863	209,244
	10 20 30 40 50 60 MIEDKGPRVA DYFVVAGLTD VSKPLEEEIH FNDACHKVAK PKEPITDVSV IIKSLGEEVP 70 80 90 100 110 120 QDYICIDVTP TGLSADLNNG SLVGPQIYLC YRRGRDKPPL TDLGVLYDWK ERLKQGCEII 130 140 150 160 170 180 QSTPYGRPAN ISGSTSSQRI YITYRRASEN MTQNTLAVTD ICIIIPSKGE SPPHTFCKVD 190 200 210 220 230 240 KNLNNSMWGS AVYLCYKKSV AKTNTVSYKA GLICRYPQED YESFSLPESV PLFCLPMGAT 250 260 270 280 290 300 IECWPSNSKY PLPVFSTFVL TGASAEKVYG AAIQFYEPYS EENLTEKQRL LLGLTSADGK 310 320 330 340 350 360 SDSSKTIHTN KCICLLSHWP FFDAFRKFLT FLYRYSISGP HVLPIEKHIS HFMHKVPFPS 370 380 390 400 410 420 PQRPRILVQL SPHDNLILSQ PVSSPLPLSG GKFSTLLQNL GPENAVTLLV FAVTEHKILI 430 440 450 460 470 480 HSLRPSVLTS VTEALVSMIF PFHWPCPYVP LCPLALADVL SAPCPFIVGI DSRYFDLYDP 490 500 510 520 530 540 PPDVSCVDVD TNTISQIGDK KNVAWKILPK KPCKNLMNTL NNLHQQLAKL QQRPRDDGLM 550 560 570 580 590 600 DLAINDYDFN SGKRLHMIDL EIQEAFLFFM ASILKGYRSY LRPITQAPSE TATDAASLFA 610 620 630 640 650 660 LQAFLRSRDR SHQKFYNMMT KTQMFIRFIE ECSFVSDKDA SLAFFDDCVD KVDMDKSGEV 670 680 690 700 710 720 RLIELDESFK SEHTVFVTPP EIPHLPNGEE PPLQYSYNGF PVLRNNLFER PEGFLQAKKN 730 740 750 760 770 780 KLPSKSSSPN SPLPMFRRTK QEIKSAHKIA KRYSSIPQMW SRCLLRHCYG LWFICLPAYV 790 800 810 820 830 840 KVCHSKVRAL KTAYDVLKKM QSKKMDPPDE VCYRILMQLC GQYDQPVLAV RVLFEMQKAG 850 860 870 880 890 900 IDPNAITYGY YNKAVLESTW PSRSRSGYFL WTKVRNVVLG VTQFKRALKK HAHLSQTTLS 910 920 930 940 950 960 GGQSDLGYNS LSKDEVRRGD TSTEDIQEEK DKKGSDCSSL SESESTKGSA DCLPKLSYQN 970 980 990 1000 1010 1020 SSSIVRLTGT SNNSAGKISG ESMESTPELL LISSLEDTNE TRNIQSRCFR KRHKSDNETN 1030 1040 1050 1060 1070 1080 LQQQVVWGNR NRNLSGGVLM GFMLNRINQE ATPGDIVEKL GADAKILSNV ISKSTRPNTL 1090 1100 1110 1120 1130 1140 DIGKPPLRSK RDSLEKESSD DDTPFDGSNY LADKVDSPVI FDLEDLDSET DVSKAGCVAT 1150 1160 1170 1180 1190 1200 QNPKRIQRMN SSFSVKPFEK TDVATGFDPL SLLVAETEQQ QKEEEEEDED DSKSISTPSA 1210 1220 1230 1240 1250 1260 RRDLAEEIVM YMNNMSSPLT SRTPSIDLQR ACDDKLNKKS PPLVKACRRS SLPPNSPKPV 1270 1280 1290 1300 1310 1320 RLTKSKSYTK SEEKPRDRLW SSPAFSPTCP FREESQDTLT HSSPSFNLDT LLVPKLDVLR 1330 1340 1350 1360 1370 1380 NSMFTAGKGV AEKASKWYSR FTMYTTSSKD QSSDRTSLSS VGAQDSESTS LTDEDVCHEL 1390 1400 1410 1420 1430 1440 EGPISSQETS ATSGTKRIDL SRISLESSAS LEGSLSKFAL PGKSEVTSSF NASNTNIFQN 1450 1460 1470 1480 1490 1500 YAMEVLISSC SRCRTCDCLV HDEEIMAGWT ADDSNLNTTC PFCGNIFLPF LNIEIRDLRR 1510 1520 1530 1540 1550 1560 PGRYFLKSSP STENMHFPSS ISSQTRQSCI STSASGLDTS ALSVQGNFDL NSKSKLQENF 1570 1580 1590 1600 1610 1620 CTRSIQIPAN RSKTAMSKCP IFPMARSIST SGPLDKEDTG RQKLISTGSL PATLQGATDS 1630 1640 1650 1660 1670 1680 LGLEWHLPSP DPVTVPYLSP LVVWKELESL LENEGDHAIT VADFVDHHPI VFWNLVWYFR 1690 1700 1710 1720 1730 1740 RLDLPSNLPG LILSSEHCNK YSKIPRHCMS EDSKYVLIQM LWDNMKLHQD PGQPLYILWN 1750 1760 1770 1780 1790 1800 AHTQKYPMVH LLQKSDNSFN QELLKSMVKS IKMNDVYGPM SQILETLNKC PHFKRQRSLY 1810 1820 1830 1840 1850 1860 REILFLSLVA LGRENIDIDA FDKEYKMAYD RLTPSQVKST HNCDRPPSTG VMECRKTFGE PYL

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Molecular cloning, structural analysis, and expression of a human IRLB, MYC promoter-binding protein: new DENN domain-containing protein family emerges." Semova N., Kapanadze B., Corcoran M., Kutsenko A., Baranova A., Semov A. Genomics 82:343-354(2003) [PubMed: 12906859] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA], TISSUE SPECIFICITY, DEVELOPMENTAL STAGE, INDUCTION.
[2]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed: 15489334] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA], VARIANT PRO-284. Tissue: Uterus.
[3]	"Complete sequencing and characterization of 21,243 full-length human cDNAs." Ota T., Suzuki Y., Nishikawa T., Otsuki T., Sugiyama T., Irie R., Wakamatsu A., Hayashi K., Sato H., Nagai K., Kimura K., Makita H., Sekine M., Obayashi M., Nishi T., Shibahara T., Tanaka T., Ishii S. , Yamamoto J., Saito K., Kawai Y., Isono Y., Nakamura Y., Nagahari K., Murakami K., Yasuda T., Iwayanagi T., Wagatsuma M., Shiratori A., Sudo H., Hosoiri T., Kaku Y., Kodaira H., Kondo H., Sugawara M., Takahashi M., Kanda K., Yokoi T., Furuya T., Kikkawa E., Omura Y., Abe K., Kamihara K., Katsuta N., Sato K., Tanikawa M., Yamazaki M., Ninomiya K., Ishibashi T., Yamashita H., Murakawa K., Fujimori K., Tanai H., Kimata M., Watanabe M., Hiraoka S., Chiba Y., Ishida S., Ono Y., Takiguchi S., Watanabe S., Yosida M., Hotuta T., Kusano J., Kanehori K., Takahashi-Fujii A., Hara H., Tanase T.-O., Nomura Y., Togiya S., Komai F., Hara R., Takeuchi K., Arita M., Imose N., Musashino K., Yuuki H., Oshima A., Sasaki N., Aotsuka S., Yoshikawa Y., Matsunawa H., Ichihara T., Shiohata N., Sano S., Moriya S., Momiyama H., Satoh N., Takami S., Terashima Y., Suzuki O., Nakagawa S., Senoh A., Mizoguchi H., Goto Y., Shimizu F., Wakebe H., Hishigaki H., Watanabe T., Sugiyama A., Takemoto M., Kawakami B., Yamazaki M., Watanabe K., Kumagai A., Itakura S., Fukuzumi Y., Fujimori Y., Komiyama M., Tashiro H., Tanigami A., Fujiwara T., Ono T., Yamada K., Fujii Y., Ozaki K., Hirao M., Ohmori Y., Kawabata A., Hikiji T., Kobatake N., Inagaki H., Ikema Y., Okamoto S., Okitani R., Kawakami T., Noguchi S., Itoh T., Shigeta K., Senba T., Matsumura K., Nakajima Y., Mizuno T., Morinaga M., Sasaki M., Togashi T., Oyama M., Hata H., Watanabe M., Komatsu T., Mizushima-Sugano J., Satoh T., Shirai Y., Takahashi Y., Nakagawa K., Okumura K., Nagase T., Nomura N., Kikuchi H., Masuho Y., Yamashita R., Nakai K., Yada T., Nakamura Y., Ohara O., Isogai T., Sugano S. Nat. Genet. 36:40-45(2004) [PubMed: 14702039] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 1-928. Tissue: Brain.
[4]	The German cDNA consortium Submitted (JUN-2003) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 33-1863. Tissue: Spinal cord.
[5]	"Cloning of a cDNA encoding a human protein which binds a sequence in the c-myc gene similar to the interferon-stimulated response element." Stasiv Y.Z., Mashkova T.D., Chernov B.K., Sokolava I.V., Itkes A.V., Kisselev L.L. Gene 145:267-272(1994) [PubMed: 8056341] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 1673-1863, FUNCTION.
[6]	"Combining protein-based IMAC, peptide-based IMAC, and MudPIT for efficient phosphoproteomic analysis." Cantin G.T., Yi W., Lu B., Park S.K., Xu T., Lee J.-D., Yates J.R. III J. Proteome Res. 7:1346-1351(2008) [PubMed: 18220336] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-1281 AND SER-1511, MASS SPECTROMETRY.
[7]	"A quantitative atlas of mitotic phosphorylation." Dephoure N., Zhou C., Villen J., Beausoleil S.A., Bakalarski C.E., Elledge S.J., Gygi S.P. Proc. Natl. Acad. Sci. U.S.A. 105:10762-10767(2008) [PubMed: 18669648] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-728; SER-731 AND SER-1587, MASS SPECTROMETRY.

Cross-references

Sequence databases
EMBLGenBankDDBJ	AF534403 mRNA. Translation: AAQ10514.1. BC041706 mRNA. Translation: AAH41706.1. AK091368 mRNA. Translation: BAC03648.1. AL832602 mRNA. Translation: CAD89960.1. X63417 mRNA. Translation: CAA45013.1.
PIR	I37904.
RefSeq	NP_005839.2.
UniGene	Hs.654567
3D structure databases
ModBase	Search...
Protein-protein interaction databases
IntAct	Q7Z401.
PTM databases
PhosphoSite	Q7Z401.
Genome annotation databases
Ensembl	ENSG00000174485. Homo sapiens. [Contig view]
GeneID	10260.
KEGG	hsa:10260.
Organism-specific databases
H-InvDB	HIX0001093. HIX0012356.
HGNC	HGNC:24321. DENND4A.
MIM	600382. gene.
GenAtlas	Search...
GeneCards	Search...
Phylogenomic databases
HOGENOM	Q7Z401.
HOVERGEN	Q7Z401.
Gene expression databases
ArrayExpress	Q7Z401.
CleanEx	HS_DENND4A.
GermOnline	ENSG00000174485. Homo sapiens.
Family and domain databases
InterPro	IPR005112. dDENN. IPR001194. DENN. IPR002885. PPR. IPR005113. uDENN. [Graphical view]
Pfam	PF03455. dDENN. 1 hit. PF02141. DENN. 1 hit. PF01535. PPR. 1 hit. PF03456. uDENN. 1 hit. [Graphical view]
SMART	SM00801. dDENN. 1 hit. SM00799. DENN. 1 hit. SM00800. uDENN. 1 hit. [Graphical view]
TIGRFAMs	TIGR00756. PPR. 1 hit.
PROSITE	PS50947. DDENN. 1 hit. PS50211. DENN. 1 hit. PS51375. PPR. 1 hit. PS50946. UDENN. 1 hit. [Graphical view]
ProtoNet	Search...
Other Resources
NextBio	38872.
SOURCE	Search...

Entry information

Entry name

MYCPP_HUMAN

Accession

Primary (citable) accession number: Q7Z401
Secondary accession number(s): Q14655 , Q86T77, Q8IVX2, Q8NB93

Entry history

Integrated into UniProtKB/Swiss-Prot:	February 21, 2006
Last sequence update:	February 21, 2006
Last modified:	November 25, 2008
This is version 42 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HPI (Human Proteome Initiative)