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Protein

C-myc promoter-binding protein

Gene

DENND4A

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

Probable guanine nucleotide exchange factor (GEF) which may activate RAB10. Promotes the exchange of GDP to GTP, converting inactive GDP-bound Rab proteins into their active GTP-bound form. According to PubMed:8056341, it may bind to ISRE-like element (interferon-stimulated response element) of MYC P2 promoter.2 Publications

GO - Molecular functioni

  • DNA binding Source: UniProtKB
  • Rab guanyl-nucleotide exchange factor activity Source: UniProtKB

GO - Biological processi

  • regulation of transcription, DNA-templated Source: UniProtKB
  • transcription, DNA-templated Source: UniProtKB-KW
Complete GO annotation...

Keywords - Molecular functioni

Guanine-nucleotide releasing factor

Keywords - Biological processi

Transcription, Transcription regulation

Keywords - Ligandi

DNA-binding

Enzyme and pathway databases

ReactomeiR-HSA-8876198. RAB GEFs exchange GTP for GDP on RABs.

Names & Taxonomyi

Protein namesi
Recommended name:
C-myc promoter-binding protein
Alternative name(s):
DENN domain-containing protein 4A
Gene namesi
Name:DENND4A
Synonyms:IRLB, MYCPBP
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
Proteomesi
  • UP000005640 Componenti: Chromosome 15

Organism-specific databases

HGNCiHGNC:24321. DENND4A.

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Nucleus

Pathology & Biotechi

Organism-specific databases

DisGeNETi10260.
OpenTargetsiENSG00000174485.
PharmGKBiPA134887513.

Polymorphism and mutation databases

BioMutaiDENND4A.
DMDMi88909230.

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00002239521 – 1863C-myc promoter-binding proteinAdd BLAST1863

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei731PhosphoserineCombined sources1
Modified residuei1015PhosphoserineCombined sources1
Modified residuei1035PhosphoserineCombined sources1
Modified residuei1099PhosphoserineCombined sources1
Modified residuei1151PhosphoserineCombined sources1
Modified residuei1152PhosphoserineCombined sources1
Modified residuei1225PhosphoserineCombined sources1
Modified residuei1240PhosphoserineCombined sources1
Modified residuei1251PhosphoserineCombined sources1
Modified residuei1281PhosphoserineCombined sources1
Modified residuei1508PhosphoserineCombined sources1
Modified residuei1587PhosphoserineCombined sources1
Modified residuei1589PhosphoserineCombined sources1
Modified residuei1591PhosphoserineCombined sources1

Keywords - PTMi

Phosphoprotein

Proteomic databases

EPDiQ7Z401.
MaxQBiQ7Z401.
PaxDbiQ7Z401.
PeptideAtlasiQ7Z401.
PRIDEiQ7Z401.

PTM databases

iPTMnetiQ7Z401.
PhosphoSitePlusiQ7Z401.

Expressioni

Tissue specificityi

Expressed ubiquitously. Highest expression in bone marrow, medium in peripheral blood lymphocytes and lowest in spleen. In brain, breast, and prostate, higher expression was seen in normal cells than in tumor cells. Expression is regulated in a growth- and cell cycle-dependent manner.1 Publication

Developmental stagei

Highly expressed in fetal liver.1 Publication

Inductioni

By serum in low-passage fibroblasts.1 Publication

Gene expression databases

BgeeiENSG00000174485.
CleanExiHS_DENND4A.
ExpressionAtlasiQ7Z401. baseline and differential.
GenevisibleiQ7Z401. HS.

Organism-specific databases

HPAiHPA065343.

Interactioni

Protein-protein interaction databases

BioGridi115552. 15 interactors.
IntActiQ7Z401. 5 interactors.
MINTiMINT-1631757.
STRINGi9606.ENSP00000391167.

Structurei

3D structure databases

ProteinModelPortaliQ7Z401.
SMRiQ7Z401.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini42 – 200MABPPROSITE-ProRule annotationAdd BLAST159
Domaini203 – 276UDENNAdd BLAST74
Domaini308 – 492DENNPROSITE-ProRule annotationAdd BLAST185
Domaini558 – 632dDENNPROSITE-ProRule annotationAdd BLAST75
Repeati772 – 808PPR 1Add BLAST37
Repeati809 – 843PPR 2Add BLAST35

Motif

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Motifi917 – 933Bipartite nuclear localization signalSequence analysisAdd BLAST17

Compositional bias

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Compositional biasi1183 – 1189Poly-Glu7

Sequence similaritiesi

Contains 1 dDENN domain.PROSITE-ProRule annotation
Contains 1 DENN domain.PROSITE-ProRule annotation
Contains 1 MABP domain.PROSITE-ProRule annotation
Contains 2 PPR (pentatricopeptide) repeats.PROSITE-ProRule annotation
Contains 1 uDENN domain.PROSITE-ProRule annotation

Keywords - Domaini

Repeat

Phylogenomic databases

eggNOGiKOG2127. Eukaryota.
ENOG410XQMX. LUCA.
GeneTreeiENSGT00760000118819.
HOGENOMiHOG000231590.
HOVERGENiHBG058313.
InParanoidiQ7Z401.
KOiK20163.
OMAiFLCFMAS.
OrthoDBiEOG091G00CV.
PhylomeDBiQ7Z401.
TreeFamiTF313237.

Family and domain databases

InterProiIPR005112. dDENN_dom.
IPR001194. DENN_dom.
IPR023341. MABP.
IPR002885. Pentatricopeptide_repeat.
IPR005113. uDENN_dom.
[Graphical view]
PfamiPF03455. dDENN. 1 hit.
PF02141. DENN. 1 hit.
PF03456. uDENN. 1 hit.
[Graphical view]
SMARTiSM00801. dDENN. 1 hit.
SM00799. DENN. 1 hit.
SM00800. uDENN. 1 hit.
[Graphical view]
TIGRFAMsiTIGR00756. PPR. 1 hit.
PROSITEiPS50947. DDENN. 1 hit.
PS50211. DENN. 1 hit.
PS51498. MABP. 1 hit.
PS51375. PPR. 1 hit.
PS50946. UDENN. 1 hit.
[Graphical view]

Sequences (2)i

Sequence statusi: Complete.

This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 1 (identifier: Q7Z401-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MIEDKGPRVA DYFVVAGLTD VSKPLEEEIH FNDACHKVAK PKEPITDVSV
60 70 80 90 100
IIKSLGEEVP QDYICIDVTP TGLSADLNNG SLVGPQIYLC YRRGRDKPPL
110 120 130 140 150
TDLGVLYDWK ERLKQGCEII QSTPYGRPAN ISGSTSSQRI YITYRRASEN
160 170 180 190 200
MTQNTLAVTD ICIIIPSKGE SPPHTFCKVD KNLNNSMWGS AVYLCYKKSV
210 220 230 240 250
AKTNTVSYKA GLICRYPQED YESFSLPESV PLFCLPMGAT IECWPSNSKY
260 270 280 290 300
PLPVFSTFVL TGASAEKVYG AAIQFYEPYS EENLTEKQRL LLGLTSADGK
310 320 330 340 350
SDSSKTIHTN KCICLLSHWP FFDAFRKFLT FLYRYSISGP HVLPIEKHIS
360 370 380 390 400
HFMHKVPFPS PQRPRILVQL SPHDNLILSQ PVSSPLPLSG GKFSTLLQNL
410 420 430 440 450
GPENAVTLLV FAVTEHKILI HSLRPSVLTS VTEALVSMIF PFHWPCPYVP
460 470 480 490 500
LCPLALADVL SAPCPFIVGI DSRYFDLYDP PPDVSCVDVD TNTISQIGDK
510 520 530 540 550
KNVAWKILPK KPCKNLMNTL NNLHQQLAKL QQRPRDDGLM DLAINDYDFN
560 570 580 590 600
SGKRLHMIDL EIQEAFLFFM ASILKGYRSY LRPITQAPSE TATDAASLFA
610 620 630 640 650
LQAFLRSRDR SHQKFYNMMT KTQMFIRFIE ECSFVSDKDA SLAFFDDCVD
660 670 680 690 700
KVDMDKSGEV RLIELDESFK SEHTVFVTPP EIPHLPNGEE PPLQYSYNGF
710 720 730 740 750
PVLRNNLFER PEGFLQAKKN KLPSKSSSPN SPLPMFRRTK QEIKSAHKIA
760 770 780 790 800
KRYSSIPQMW SRCLLRHCYG LWFICLPAYV KVCHSKVRAL KTAYDVLKKM
810 820 830 840 850
QSKKMDPPDE VCYRILMQLC GQYDQPVLAV RVLFEMQKAG IDPNAITYGY
860 870 880 890 900
YNKAVLESTW PSRSRSGYFL WTKVRNVVLG VTQFKRALKK HAHLSQTTLS
910 920 930 940 950
GGQSDLGYNS LSKDEVRRGD TSTEDIQEEK DKKGSDCSSL SESESTKGSA
960 970 980 990 1000
DCLPKLSYQN SSSIVRLTGT SNNSAGKISG ESMESTPELL LISSLEDTNE
1010 1020 1030 1040 1050
TRNIQSRCFR KRHKSDNETN LQQQVVWGNR NRNLSGGVLM GFMLNRINQE
1060 1070 1080 1090 1100
ATPGDIVEKL GADAKILSNV ISKSTRPNTL DIGKPPLRSK RDSLEKESSD
1110 1120 1130 1140 1150
DDTPFDGSNY LADKVDSPVI FDLEDLDSET DVSKAGCVAT QNPKRIQRMN
1160 1170 1180 1190 1200
SSFSVKPFEK TDVATGFDPL SLLVAETEQQ QKEEEEEDED DSKSISTPSA
1210 1220 1230 1240 1250
RRDLAEEIVM YMNNMSSPLT SRTPSIDLQR ACDDKLNKKS PPLVKACRRS
1260 1270 1280 1290 1300
SLPPNSPKPV RLTKSKSYTK SEEKPRDRLW SSPAFSPTCP FREESQDTLT
1310 1320 1330 1340 1350
HSSPSFNLDT LLVPKLDVLR NSMFTAGKGV AEKASKWYSR FTMYTTSSKD
1360 1370 1380 1390 1400
QSSDRTSLSS VGAQDSESTS LTDEDVCHEL EGPISSQETS ATSGTKRIDL
1410 1420 1430 1440 1450
SRISLESSAS LEGSLSKFAL PGKSEVTSSF NASNTNIFQN YAMEVLISSC
1460 1470 1480 1490 1500
SRCRTCDCLV HDEEIMAGWT ADDSNLNTTC PFCGNIFLPF LNIEIRDLRR
1510 1520 1530 1540 1550
PGRYFLKSSP STENMHFPSS ISSQTRQSCI STSASGLDTS ALSVQGNFDL
1560 1570 1580 1590 1600
NSKSKLQENF CTRSIQIPAN RSKTAMSKCP IFPMARSIST SGPLDKEDTG
1610 1620 1630 1640 1650
RQKLISTGSL PATLQGATDS LGLEWHLPSP DPVTVPYLSP LVVWKELESL
1660 1670 1680 1690 1700
LENEGDHAIT VADFVDHHPI VFWNLVWYFR RLDLPSNLPG LILSSEHCNK
1710 1720 1730 1740 1750
YSKIPRHCMS EDSKYVLIQM LWDNMKLHQD PGQPLYILWN AHTQKYPMVH
1760 1770 1780 1790 1800
LLQKSDNSFN QELLKSMVKS IKMNDVYGPM SQILETLNKC PHFKRQRSLY
1810 1820 1830 1840 1850
REILFLSLVA LGRENIDIDA FDKEYKMAYD RLTPSQVKST HNCDRPPSTG
1860
VMECRKTFGE PYL
Length:1,863
Mass (Da):209,244
Last modified:February 21, 2006 - v2
Checksum:i7432EEC9DB9711E6
GO
Isoform 2 (identifier: Q7Z401-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     900-900: S → SDGSDLDAVSHGSMDSGHGTHTVEQAPFNTGLIKVYATDDRSST

Note: No experimental confirmation available.
Show »
Length:1,906
Mass (Da):213,704
Checksum:i4A2E0BB91759E401
GO

Sequence cautioni

The sequence AL833317 differs from that shown. Reason: Frameshift at position 1774.Curated

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti256S → P in AL833317 (PubMed:17974005).Curated1
Sequence conflicti383S → P in CAD89960 (PubMed:17974005).Curated1
Sequence conflicti983M → MGK in AAQ10514 (PubMed:12906859).Curated1
Sequence conflicti1532T → A in AL833317 (PubMed:17974005).Curated1
Sequence conflicti1726K → Q in AL833317 (PubMed:17974005).Curated1

Natural variant

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Natural variantiVAR_025362284L → P.1 PublicationCorresponds to variant rs17854146dbSNPEnsembl.1

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_044630900S → SDGSDLDAVSHGSMDSGHGT HTVEQAPFNTGLIKVYATDD RSST in isoform 2. 1 Publication1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF534403 mRNA. Translation: AAQ10514.1.
AL832602 mRNA. Translation: CAD89960.1.
AL833317 mRNA. No translation available.
AC011939 Genomic DNA. No translation available.
BC041706 mRNA. Translation: AAH41706.1.
AK091368 mRNA. Translation: BAC03648.1.
X63417 mRNA. Translation: CAA45013.1.
CCDSiCCDS45285.1. [Q7Z401-1]
CCDS53949.1. [Q7Z401-2]
PIRiI37904.
RefSeqiNP_001138295.1. NM_001144823.2. [Q7Z401-2]
NP_005839.3. NM_005848.4. [Q7Z401-1]
XP_005254178.1. XM_005254121.3. [Q7Z401-1]
XP_016877352.1. XM_017021863.1. [Q7Z401-1]
UniGeneiHs.654567.

Genome annotation databases

EnsembliENST00000431932; ENSP00000396830; ENSG00000174485. [Q7Z401-1]
ENST00000443035; ENSP00000391167; ENSG00000174485. [Q7Z401-2]
GeneIDi10260.
KEGGihsa:10260.
UCSCiuc002aph.4. human. [Q7Z401-1]

Keywords - Coding sequence diversityi

Alternative splicing, Polymorphism

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF534403 mRNA. Translation: AAQ10514.1.
AL832602 mRNA. Translation: CAD89960.1.
AL833317 mRNA. No translation available.
AC011939 Genomic DNA. No translation available.
BC041706 mRNA. Translation: AAH41706.1.
AK091368 mRNA. Translation: BAC03648.1.
X63417 mRNA. Translation: CAA45013.1.
CCDSiCCDS45285.1. [Q7Z401-1]
CCDS53949.1. [Q7Z401-2]
PIRiI37904.
RefSeqiNP_001138295.1. NM_001144823.2. [Q7Z401-2]
NP_005839.3. NM_005848.4. [Q7Z401-1]
XP_005254178.1. XM_005254121.3. [Q7Z401-1]
XP_016877352.1. XM_017021863.1. [Q7Z401-1]
UniGeneiHs.654567.

3D structure databases

ProteinModelPortaliQ7Z401.
SMRiQ7Z401.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi115552. 15 interactors.
IntActiQ7Z401. 5 interactors.
MINTiMINT-1631757.
STRINGi9606.ENSP00000391167.

PTM databases

iPTMnetiQ7Z401.
PhosphoSitePlusiQ7Z401.

Polymorphism and mutation databases

BioMutaiDENND4A.
DMDMi88909230.

Proteomic databases

EPDiQ7Z401.
MaxQBiQ7Z401.
PaxDbiQ7Z401.
PeptideAtlasiQ7Z401.
PRIDEiQ7Z401.

Protocols and materials databases

DNASUi10260.
Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENST00000431932; ENSP00000396830; ENSG00000174485. [Q7Z401-1]
ENST00000443035; ENSP00000391167; ENSG00000174485. [Q7Z401-2]
GeneIDi10260.
KEGGihsa:10260.
UCSCiuc002aph.4. human. [Q7Z401-1]

Organism-specific databases

CTDi10260.
DisGeNETi10260.
GeneCardsiDENND4A.
H-InvDBHIX0012356.
HGNCiHGNC:24321. DENND4A.
HPAiHPA065343.
MIMi600382. gene.
neXtProtiNX_Q7Z401.
OpenTargetsiENSG00000174485.
PharmGKBiPA134887513.
GenAtlasiSearch...

Phylogenomic databases

eggNOGiKOG2127. Eukaryota.
ENOG410XQMX. LUCA.
GeneTreeiENSGT00760000118819.
HOGENOMiHOG000231590.
HOVERGENiHBG058313.
InParanoidiQ7Z401.
KOiK20163.
OMAiFLCFMAS.
OrthoDBiEOG091G00CV.
PhylomeDBiQ7Z401.
TreeFamiTF313237.

Enzyme and pathway databases

ReactomeiR-HSA-8876198. RAB GEFs exchange GTP for GDP on RABs.

Miscellaneous databases

ChiTaRSiDENND4A. human.
GeneWikiiDENND4A.
GenomeRNAii10260.
PROiQ7Z401.
SOURCEiSearch...

Gene expression databases

BgeeiENSG00000174485.
CleanExiHS_DENND4A.
ExpressionAtlasiQ7Z401. baseline and differential.
GenevisibleiQ7Z401. HS.

Family and domain databases

InterProiIPR005112. dDENN_dom.
IPR001194. DENN_dom.
IPR023341. MABP.
IPR002885. Pentatricopeptide_repeat.
IPR005113. uDENN_dom.
[Graphical view]
PfamiPF03455. dDENN. 1 hit.
PF02141. DENN. 1 hit.
PF03456. uDENN. 1 hit.
[Graphical view]
SMARTiSM00801. dDENN. 1 hit.
SM00799. DENN. 1 hit.
SM00800. uDENN. 1 hit.
[Graphical view]
TIGRFAMsiTIGR00756. PPR. 1 hit.
PROSITEiPS50947. DDENN. 1 hit.
PS50211. DENN. 1 hit.
PS51498. MABP. 1 hit.
PS51375. PPR. 1 hit.
PS50946. UDENN. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiMYCPP_HUMAN
AccessioniPrimary (citable) accession number: Q7Z401
Secondary accession number(s): E7EPL3
, Q14655, Q86T77, Q8IVX2, Q8NB93
Entry historyi
Integrated into UniProtKB/Swiss-Prot: February 21, 2006
Last sequence update: February 21, 2006
Last modified: November 30, 2016
This is version 122 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Human chromosome 15
    Human chromosome 15: entries, gene names and cross-references to MIM
  2. Human entries with polymorphisms or disease mutations
    List of human entries with polymorphisms or disease mutations
  3. Human polymorphisms and disease mutations
    Index of human polymorphisms and disease mutations
  4. MIM cross-references
    Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
  5. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.