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Protein

Keratin, type I cytoskeletal 28

Gene

KRT28

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

Essential for the proper assembly of types I and II keratin protein complexes and the formation of keratin intermediate filaments in the inner root sheath (irs).By similarity

GO - Molecular functioni

Complete GO annotation...

Names & Taxonomyi

Protein namesi
Recommended name:
Keratin, type I cytoskeletal 28
Alternative name(s):
Cytokeratin-28
Short name:
CK-28
Keratin-25D
Short name:
K25D
Keratin-28
Short name:
K28
Type I inner root sheath-specific keratin-K25irs4
Gene namesi
Name:KRT28Imported
Synonyms:KRT25DImported
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
Proteomesi
  • UP000005640 Componenti: Chromosome 17

Organism-specific databases

HGNCiHGNC:30842. KRT28.

Subcellular locationi

  • Cytoplasm By similarity

GO - Cellular componenti

  • cytoplasm Source: UniProtKB-SubCell
  • extracellular exosome Source: UniProtKB
  • intermediate filament Source: UniProtKB-KW
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm, Intermediate filament, Keratin

Pathology & Biotechi

Organism-specific databases

PharmGKBiPA134871316.

Polymorphism and mutation databases

BioMutaiKRT28.
DMDMi294862446.

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 464464Keratin, type I cytoskeletal 28PRO_0000312705Add
BLAST

Proteomic databases

EPDiQ7Z3Y7.
PaxDbiQ7Z3Y7.
PRIDEiQ7Z3Y7.

PTM databases

PhosphoSiteiQ7Z3Y7.

Expressioni

Tissue specificityi

Strongly expressed in skin and scalp, and weak expression observed in thymus. In the hair follicle, expressed in Henle layer, Huxley layer and in the irs cuticle. Expression extends from the bulb region up to the point of differentiation into the three layers. Also present in the medulla of beard hair (at protein level).2 Publications

Gene expression databases

BgeeiQ7Z3Y7.
CleanExiHS_KRT28.

Interactioni

Subunit structurei

Heterotetramer of two type I and two type II keratins.Curated

Protein-protein interaction databases

BioGridi127825. 1 interaction.

Structurei

3D structure databases

ProteinModelPortaliQ7Z3Y7.
SMRiQ7Z3Y7. Positions 140-236, 307-396.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni1 – 8585HeadSequence analysisAdd
BLAST
Regioni86 – 397312RodSequence analysisAdd
BLAST
Regioni86 – 12136Coil 1ASequence analysisAdd
BLAST
Regioni122 – 14322Linker 1Sequence analysisAdd
BLAST
Regioni144 – 23592Coil 1BSequence analysisAdd
BLAST
Regioni236 – 25823Linker 12Sequence analysisAdd
BLAST
Regioni259 – 397139Coil 2Sequence analysisAdd
BLAST
Regioni398 – 46467TailSequence analysisAdd
BLAST

Compositional bias

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Compositional biasi17 – 8468Gly-richSequence analysisAdd
BLAST

Sequence similaritiesi

Belongs to the intermediate filament family.Sequence analysis

Keywords - Domaini

Coiled coil

Phylogenomic databases

eggNOGiENOG410IFTF. Eukaryota.
ENOG410Y9IV. LUCA.
GeneTreeiENSGT00760000118808.
HOGENOMiHOG000230975.
HOVERGENiHBG013015.
InParanoidiQ7Z3Y7.
KOiK07604.
OMAiELQFESL.
OrthoDBiEOG7FV3Q8.
PhylomeDBiQ7Z3Y7.
TreeFamiTF332742.

Family and domain databases

InterProiIPR001664. IF.
IPR002957. Keratin_I.
[Graphical view]
PANTHERiPTHR23239. PTHR23239. 1 hit.
PfamiPF00038. Filament. 1 hit.
[Graphical view]
PRINTSiPR01248. TYPE1KERATIN.
SMARTiSM01391. Filament. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q7Z3Y7-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSLQFSNGSR HVCLRSGAGS VRPLNGGAGF AGSSACGGSV AGSEFSCALG
60 70 80 90 100
GGLGSVPGGS HAGGALGNAA CIGFAGSEGG LLSGNEKVTM QNLNDRLASY
110 120 130 140 150
LDNVRALEEA NAELERKIKG WYEKYGPGSC RGLDHDYSRY HLTIEDLKNK
160 170 180 190 200
IISSTTTNAN VILQIDNARL AADDFRLKYE NELTLHQNVE ADINGLRRVL
210 220 230 240 250
DELTLCRTDQ ELQYESLSEE MTYLKKNHEE EMKALQCAAG GNVNVEMNAA
260 270 280 290 300
PGVDLAVLLN NMRAEYEALA EQNRKDAEAW FNEKSASLQQ QISHDSGAAT
310 320 330 340 350
FARSQLTEMR RTLQTLEIQL QSLMATKHSL ECSLTETESN YCTQLAQIQA
360 370 380 390 400
QIGALEEQLH QVRTETEGQK LEYEHLLDVK VHLEKEIETY CRLIDGDGNS
410 420 430 440 450
CSKSKGFGSG SPGNSSKDLS KTTLVKTVVE ELDQRGKVLS SRIHSIEEKT
460
SKMTNGKTEQ RVPF
Length:464
Mass (Da):50,567
Last modified:April 20, 2010 - v2
Checksum:i34BA7243B68EF42C
GO

Sequence cautioni

The sequence BAC85240.1 differs from that shown. Reason: Erroneous initiation. Curated

Natural variant

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Natural varianti52 – 521G → D.
Corresponds to variant rs7209228 [ dbSNP | Ensembl ].
VAR_056009
Natural varianti62 – 621A → V.1 Publication
Corresponds to variant rs4624233 [ dbSNP | Ensembl ].
VAR_060236
Natural varianti286 – 2861A → T.
Corresponds to variant rs17558995 [ dbSNP | Ensembl ].
VAR_056010

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AJ564207 mRNA. Translation: CAD91907.1.
AK129827 mRNA. Translation: BAC85240.1. Different initiation.
AC090283 Genomic DNA. No translation available.
CCDSiCCDS11376.1.
RefSeqiNP_853513.2. NM_181535.3.
UniGeneiHs.59736.

Genome annotation databases

EnsembliENST00000306658; ENSP00000305263; ENSG00000173908.
GeneIDi162605.
KEGGihsa:162605.
UCSCiuc002hvh.2. human.

Keywords - Coding sequence diversityi

Polymorphism

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AJ564207 mRNA. Translation: CAD91907.1.
AK129827 mRNA. Translation: BAC85240.1. Different initiation.
AC090283 Genomic DNA. No translation available.
CCDSiCCDS11376.1.
RefSeqiNP_853513.2. NM_181535.3.
UniGeneiHs.59736.

3D structure databases

ProteinModelPortaliQ7Z3Y7.
SMRiQ7Z3Y7. Positions 140-236, 307-396.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi127825. 1 interaction.

PTM databases

PhosphoSiteiQ7Z3Y7.

Polymorphism and mutation databases

BioMutaiKRT28.
DMDMi294862446.

Proteomic databases

EPDiQ7Z3Y7.
PaxDbiQ7Z3Y7.
PRIDEiQ7Z3Y7.

Protocols and materials databases

DNASUi162605.
Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENST00000306658; ENSP00000305263; ENSG00000173908.
GeneIDi162605.
KEGGihsa:162605.
UCSCiuc002hvh.2. human.

Organism-specific databases

CTDi162605.
GeneCardsiKRT28.
HGNCiHGNC:30842. KRT28.
MIMi616677. gene.
neXtProtiNX_Q7Z3Y7.
PharmGKBiPA134871316.
GenAtlasiSearch...

Phylogenomic databases

eggNOGiENOG410IFTF. Eukaryota.
ENOG410Y9IV. LUCA.
GeneTreeiENSGT00760000118808.
HOGENOMiHOG000230975.
HOVERGENiHBG013015.
InParanoidiQ7Z3Y7.
KOiK07604.
OMAiELQFESL.
OrthoDBiEOG7FV3Q8.
PhylomeDBiQ7Z3Y7.
TreeFamiTF332742.

Miscellaneous databases

GenomeRNAii162605.
NextBioi88200.
PROiQ7Z3Y7.
SOURCEiSearch...

Gene expression databases

BgeeiQ7Z3Y7.
CleanExiHS_KRT28.

Family and domain databases

InterProiIPR001664. IF.
IPR002957. Keratin_I.
[Graphical view]
PANTHERiPTHR23239. PTHR23239. 1 hit.
PfamiPF00038. Filament. 1 hit.
[Graphical view]
PRINTSiPR01248. TYPE1KERATIN.
SMARTiSM01391. Filament. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "The human type I keratin gene family: characterization of new hair follicle specific members and evaluation of the chromosome 17q21.2 gene domain."
    Rogers M.A., Winter H., Langbein L., Bleiler R., Schweizer J.
    Differentiation 72:527-540(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA], VARIANT VAL-62, TISSUE SPECIFICITY.
    Tissue: ScalpImported.
  2. "Complete sequencing and characterization of 21,243 full-length human cDNAs."
    Ota T., Suzuki Y., Nishikawa T., Otsuki T., Sugiyama T., Irie R., Wakamatsu A., Hayashi K., Sato H., Nagai K., Kimura K., Makita H., Sekine M., Obayashi M., Nishi T., Shibahara T., Tanaka T., Ishii S.
    , Yamamoto J., Saito K., Kawai Y., Isono Y., Nakamura Y., Nagahari K., Murakami K., Yasuda T., Iwayanagi T., Wagatsuma M., Shiratori A., Sudo H., Hosoiri T., Kaku Y., Kodaira H., Kondo H., Sugawara M., Takahashi M., Kanda K., Yokoi T., Furuya T., Kikkawa E., Omura Y., Abe K., Kamihara K., Katsuta N., Sato K., Tanikawa M., Yamazaki M., Ninomiya K., Ishibashi T., Yamashita H., Murakawa K., Fujimori K., Tanai H., Kimata M., Watanabe M., Hiraoka S., Chiba Y., Ishida S., Ono Y., Takiguchi S., Watanabe S., Yosida M., Hotuta T., Kusano J., Kanehori K., Takahashi-Fujii A., Hara H., Tanase T.-O., Nomura Y., Togiya S., Komai F., Hara R., Takeuchi K., Arita M., Imose N., Musashino K., Yuuki H., Oshima A., Sasaki N., Aotsuka S., Yoshikawa Y., Matsunawa H., Ichihara T., Shiohata N., Sano S., Moriya S., Momiyama H., Satoh N., Takami S., Terashima Y., Suzuki O., Nakagawa S., Senoh A., Mizoguchi H., Goto Y., Shimizu F., Wakebe H., Hishigaki H., Watanabe T., Sugiyama A., Takemoto M., Kawakami B., Yamazaki M., Watanabe K., Kumagai A., Itakura S., Fukuzumi Y., Fujimori Y., Komiyama M., Tashiro H., Tanigami A., Fujiwara T., Ono T., Yamada K., Fujii Y., Ozaki K., Hirao M., Ohmori Y., Kawabata A., Hikiji T., Kobatake N., Inagaki H., Ikema Y., Okamoto S., Okitani R., Kawakami T., Noguchi S., Itoh T., Shigeta K., Senba T., Matsumura K., Nakajima Y., Mizuno T., Morinaga M., Sasaki M., Togashi T., Oyama M., Hata H., Watanabe M., Komatsu T., Mizushima-Sugano J., Satoh T., Shirai Y., Takahashi Y., Nakagawa K., Okumura K., Nagase T., Nomura N., Kikuchi H., Masuho Y., Yamashita R., Nakai K., Yada T., Nakamura Y., Ohara O., Isogai T., Sugano S.
    Nat. Genet. 36:40-45(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Tissue: Dermoid cancerImported.
  3. "DNA sequence of human chromosome 17 and analysis of rearrangement in the human lineage."
    Zody M.C., Garber M., Adams D.J., Sharpe T., Harrow J., Lupski J.R., Nicholson C., Searle S.M., Wilming L., Young S.K., Abouelleil A., Allen N.R., Bi W., Bloom T., Borowsky M.L., Bugalter B.E., Butler J., Chang J.L.
    , Chen C.-K., Cook A., Corum B., Cuomo C.A., de Jong P.J., DeCaprio D., Dewar K., FitzGerald M., Gilbert J., Gibson R., Gnerre S., Goldstein S., Grafham D.V., Grocock R., Hafez N., Hagopian D.S., Hart E., Norman C.H., Humphray S., Jaffe D.B., Jones M., Kamal M., Khodiyar V.K., LaButti K., Laird G., Lehoczky J., Liu X., Lokyitsang T., Loveland J., Lui A., Macdonald P., Major J.E., Matthews L., Mauceli E., McCarroll S.A., Mihalev A.H., Mudge J., Nguyen C., Nicol R., O'Leary S.B., Osoegawa K., Schwartz D.C., Shaw-Smith C., Stankiewicz P., Steward C., Swarbreck D., Venkataraman V., Whittaker C.A., Yang X., Zimmer A.R., Bradley A., Hubbard T., Birren B.W., Rogers J., Lander E.S., Nusbaum C.
    Nature 440:1045-1049(2006) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
  4. "K25 (K25irs1), K26 (K25irs2), K27 (K25irs3), and K28 (K25irs4) represent the type I inner root sheath keratins of the human hair follicle."
    Langbein L., Rogers M.A., Praetzel-Wunder S., Helmke B., Schirmacher P., Schweizer J.
    J. Invest. Dermatol. 126:2377-2386(2006) [PubMed] [Europe PMC] [Abstract]
    Cited for: TISSUE SPECIFICITY.

Entry informationi

Entry nameiK1C28_HUMAN
AccessioniPrimary (citable) accession number: Q7Z3Y7
Secondary accession number(s): Q6ZP84
Entry historyi
Integrated into UniProtKB/Swiss-Prot: December 4, 2007
Last sequence update: April 20, 2010
Last modified: May 11, 2016
This is version 94 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Miscellaneous

There are two types of cytoskeletal and microfibrillar keratin: I (acidic; 40-55 kDa) and II (neutral to basic; 56-70 kDa).Curated

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Human chromosome 17
    Human chromosome 17: entries, gene names and cross-references to MIM
  2. Human entries with polymorphisms or disease mutations
    List of human entries with polymorphisms or disease mutations
  3. Human polymorphisms and disease mutations
    Index of human polymorphisms and disease mutations
  4. MIM cross-references
    Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
  5. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.